Composition with anti-inflammatory and anti-allergic effects and application thereof

Abstract

The invention relates to a composition with anti-inflammatory and anti-allergic effects and application thereof. The composition is prepared from the following raw materials: bolete, emblic leafflower fruit and roxburgh rose. The composition with bolete, phyllanthus emblica and roxburgh rose as preparation raw materials is prepared by coupling steam explosion with heating reflux, the extraction efficiency and the biological activity are remarkably improved, and compared with a traditional extraction technology, the method also avoids use of organic reagents, and is more environmentally friendly and safer.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of substance extraction, and relates to a composition with anti-inflammatory and anti-allergic effects and application thereof. BACKGROUND

[0002] In recent years, multiple environmental stress factors (such as dietary imbalance, pollution exposure, continuous stress and allergen stimulation) jointly induce the body to form a chronic pro-inflammatory microenvironment, triggering immune homeostasis disorder and increasing the susceptibility of allergic reactions. Studies have found that by targeting the inhibition of the release of inflammatory mediator NO and the activity of hyaluronidase, not only can the cascade activation of key inflammatory signaling pathways such as NF-κB be effectively blocked, but also the integrity of the extracellular matrix structure can be maintained, thereby significantly relieving tissue edema and allergic reactions, and then repairing the skin barrier and maintaining the immune balance of the body.

[0003] Boletus, a large wild fungus, is known as one of the "four famous fungi" due to its rich nutrition and delicious flesh. Boletus is rich in flavonoids, polysaccharides, proteins, ergosterols, minerals, vitamins, amino acids, cellulose and other active substances, and has the effects of antioxidant and immune regulation, but the application of Boletus in anti-allergy is rarely seen.

[0004] Phyllanthus emblica L., a perennial tree plant of Euphorbiaceae Phyllanthus, is a fruit of medicine and food. Modern pharmacological studies have shown that Phyllanthus emblica is rich in active ingredients such as polyphenols, polysaccharides, vitamin C and superoxide dismutase (SOD), and the fruit of Phyllanthus emblica has been proved to have potential medicinal value in antioxidant and anti-tumor aspects, and its anti-inflammatory effect is significant, and it is considered as an ideal natural anti-inflammatory drug.

[0005] Rosa Roxburghii, also known as Wenxian fruit and Sendonggui, is the fruit of a perennial deciduous shrub of Rosaceae. Rosa Roxburghii has a strong aroma and a crisp taste, and is known as the "VC King" and "Three King's Holy Fruit" due to its rich vitamin C content. Rosa Roxburghii has the effects of antioxidant, anti-inflammatory and antibacterial, and immune regulation, and has broad application prospects in the fields of food, health products, medicine and cosmetics.

[0006] Natural plant-derived anti-allergic and anti-inflammatory components have gradually become a research and application hotspot due to their high safety, mild effects, and multi-target regulation. CN119700629A and CN115737754B both disclose compositions with anti-inflammatory and anti-allergic effects. Although they differ in their composition and application focus, their formulation designs share certain commonalities, including diverse components, complex formulations, and relatively simple mechanisms of action. Furthermore, these compositions all rely on organic solvent extraction systems, leading to the risk of solvent residue in the final product, which significantly limits their market application scope. Summary of the Invention

[0007] In view of the shortcomings of the prior art, the purpose of this invention is to provide a composition with anti-inflammatory and anti-allergic effects and its application.

[0008] To achieve this objective, the present invention adopts the following technical solution:

[0009] In a first aspect, the present invention provides a composition with anti-inflammatory and anti-allergic effects, wherein the raw materials for preparing the composition include porcini mushroom, amla, and prickly pear.

[0010] This invention innovatively uses three natural plants—Boletus edulis, Phyllanthus emblica, and Rosa rugosa—to construct a composite system. Through a green extraction process, it not only completely avoids organic solvent residues, ensuring product safety, but also achieves synergistic effects of the three plants through a scientific formulation ratio. This inhibits hyaluronidase activity and the expression of pro-inflammatory factors (NO). In vivo zebrafish model anti-allergy tests have proven that the composition has both anti-inflammatory and anti-allergic effects. It can be widely used in functional foods, health foods, and other anti-inflammatory and anti-allergic products, and has broad application prospects.

[0011] Preferably, the raw materials for preparing the composition include, by mass parts, 5-20 parts of Boletus edulis, 5-20 parts of Phyllanthus emblica, and 2-10 parts of Prickly pear.

[0012] The weight percentages for Boletus can be 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, or 20 parts. The weight percentages for Phyllanthus emblica can be 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, or 20 parts. The weight percentages for Prickly Pear can be 2, 3, 4, 5, 6, 7, 8, 9, or 10 parts. Other specific values ​​within the above ranges can also be selected, and will not be elaborated on here.

[0013] Preferably, the composition is prepared by a method comprising the following steps:

[0014] (1) Boletus edulis and Phyllanthus emblica were pretreated by steam explosion.

[0015] (2) Mix the pretreated Boletus, the pretreated Phyllanthus emblica, and Prickly pear with water, and reflux to extract the extract.

[0016] (3) Filter and concentrate the extract to obtain a concentrated solution. Refrigerate the concentrated solution to flocculate it, filter it, and then dry it to obtain the final product.

[0017] Compared with traditional processes, this invention uses pure water extraction technology, completely avoiding the use of organic solvents. This not only ensures product safety and meets consumers' demand for natural and healthy products, but also reduces the risk of environmental pollution. At the same time, the process is simple, energy-efficient, and suitable for industrial production.

[0018] Preferably, the water content of the porcini mushroom is 20-30 wt%, and the water content of the amla fruit is 25-35 wt%.

[0019] The moisture content of porcini mushrooms can be selected from 20 wt%, 21 wt%, 22 wt%, 23 wt%, 24 wt%, 25 wt%, 26 wt%, 27 wt%, 28 wt%, 29 wt%, 30 wt%, etc., and the moisture content of amla can be selected from 25 wt%, 26 wt%, 27 wt%, 28 wt%, 29 wt%, 30 wt%, 31 wt%, 32 wt%, 33 wt%, 34 wt%, 35 wt%, etc. Other specific values ​​within the above range can be selected, and will not be elaborated here.

[0020] Preferably, the pressure of the bolete steam explosion is 0.4-0.8 MPa, the temperature is 140-180 ℃, and the time is 30-60 s.

[0021] The pressure can be selected from 0.4 MPa, 0.5 MPa, 0.6 MPa, 0.7 MPa, 0.8 MPa, etc., the temperature can be selected from 140℃, 150℃, 160℃, 170℃, 180℃, etc., and the time can be selected from 30 s, 32 s, 35 s, 38 s, 40 s, 42 s, 45 s, 48 ​​s, 50 s, 52 s, 55 s, 58 s, 60 s, etc. Other specific values ​​within the above range can be selected, which will not be elaborated here.

[0022] Preferably, the process of steam explosion of the porcini mushrooms further includes drying.

[0023] Preferably, the water content of the pretreated porcini mushrooms is below 8 wt%, such as 1 wt%, 2 wt%, 3 wt%, 4 wt%, 5 wt%, 6 wt%, 7 wt%, 8 wt%, etc. Other specific values ​​within the above range can be selected, and will not be elaborated here.

[0024] Preferably, the pressure of the amla steam explosion is 0.5-0.8 MPa, the temperature is 150-180 ℃, and the time is 60-100 s.

[0025] The pressure can be selected from 0.5 MPa, 0.55 MPa, 0.6 MPa, 0.65 MPa, 0.7 MPa, 0.75 MPa, 0.8 MPa, etc.; the temperature can be selected from 150 ℃, 155 ℃, 160 ℃, 165 ℃, 170 ℃, 175 ℃, 180 ℃, etc.; the time can be selected from 60 s, 65 s, 70 s, 75 s, 80 s, 85 s, 90 s, 95 s, 100 s, etc. Other specific values ​​within the above range can be selected, which will not be elaborated here.

[0026] Preferably, the process of steam explosion of the amla fruit further includes drying.

[0027] Preferably, the moisture content of the pretreated amla is 5-10 wt%, such as 5 wt%, 6 wt%, 7 wt%, 8 wt%, 9 wt%, 10 wt%, etc. Other specific values ​​within the above range can be selected, and will not be elaborated here.

[0028] Preferably, the water content of the prickly pear is 5-10 wt%, such as 5 wt%, 6 wt%, 7 wt%, 8 wt%, 9 wt%, 10 wt%, etc. Other specific values ​​within the above range can be selected, and will not be elaborated here.

[0029] Preferably, the material-to-liquid ratio for the reflux extraction is 1 g:(10-20) mL, where the specific values ​​in (10-20) can be selected from 10, 12, 14, 16, 18, 20, etc. Other specific values ​​within the above range can also be selected, and will not be elaborated here.

[0030] Preferably, the reflux extraction time is 0.5-1 h, and the number of times is 1-3.

[0031] The time can be selected as 0.5 h, 0.6 h, 0.7 h, 0.8 h, 0.9 h, 1 h, etc., and the number of times can be selected as 1 time, 2 times, 3 times, etc. Other specific point values ​​within the above range can be selected, which will not be elaborated here.

[0032] Preferably, the solid content of the concentrate is 8-12 wt%, such as 8 wt%, 9 wt%, 10 wt%, 11 wt%, 12 wt%, etc. Other specific values ​​within the above range can be selected, and will not be elaborated here.

[0033] Preferably, the refrigerated flocculation temperature is 2-6 ℃ and the time is 0.5-2 h.

[0034] Temperatures can be selected from 2 ℃, 2.5 ℃, 3 ℃, 3.5 ℃, 4 ℃, 4.5 ℃, 5 ℃, 5.5 ℃, 6 ℃, etc., and time can be selected from 0.5 h, 0.6 h, 0.8 h, 1.0 h, 1.2 h, 1.4 h, 1.6 h, 1.8 h, 2 h, etc. Other specific point values ​​within the above range can be selected, which will not be elaborated here.

[0035] In a second aspect, the present invention provides the use of the composition with anti-inflammatory and anti-allergic effects according to the first aspect in the preparation of products with anti-inflammatory and anti-allergic effects.

[0036] Preferably, the product includes food or cosmetics.

[0037] Compared with the prior art, the present invention has the following beneficial effects:

[0038] (1) The present invention uses steam explosion coupled heating reflux to prepare a composition using Boletus edulis, Phyllanthus emblica and Prickly pear as raw materials. This method not only significantly improves the extraction efficiency and biological activity, but also avoids the use of organic reagents compared with traditional extraction technology, making it more environmentally friendly and safer.

[0039] (2) The composition obtained by the preparation method of the present invention has a synergistic effect. The composition significantly improves the inhibition rate of hyaluronidase and can effectively inhibit the expression of inflammatory factor NO and trypsin in zebrafish in vivo model, and has stronger anti-allergic and anti-inflammatory effects.

[0040] (3) This invention strictly selects natural plant raw materials that are both food and medicine. All components meet the national food raw material safety standards and are prepared through green and environmentally friendly extraction processes. While ensuring the efficient extraction of active ingredients, it ensures that the product has natural, safe and nutritious characteristics, and fully meets the safety and health requirements of functional foods. Attached Figure Description

[0041] Figure 1 This is a graph showing the expression levels of trypsin in zebrafish. ### This indicates that compared with the normal control group, p < 0.001; *** This indicates that compared with the model control group, p < 0.001; **This indicates that compared with the model control group, p < 0.01; ++ This indicates a comparison with Example 1 group, p < 0.01. Detailed Implementation

[0042] The technical solution of the present invention will be further illustrated below through specific embodiments. Those skilled in the art should understand that the embodiments described are merely illustrative of the present invention and should not be construed as limiting the invention in any way.

[0043] Preparation Example 1

[0044] This preparation example provides a method for preparing a Boletus extract, the method comprising:

[0045] (1) Adjust the moisture content of Boletus edulis to 20 wt%, cut it into 2-5 cm particles, and steam explosion treatment at 0.6 MPa and 165 ℃ for 40 s, with a pressure release rate of <0.1 seconds. Circulate the explosion once, dry it to a moisture content of 4 wt%, and pulverize it to no more than 80 mesh to obtain the pretreated Boletus edulis product.

[0046] (2) Mix the pretreated Boletus product with water at a ratio of 1 g: 15 mL, heat and reflux for 45 min, extract twice, pass through a 600 mesh filter cloth and a 5 μm filter bag to obtain the extract; concentrate the extract to a solid content of 10 wt%, refrigerate at 4 ℃ for 1 h for flocculation, filter, sterilize the filtrate, and dry to obtain the final product.

[0047] Preparation Example 2

[0048] This preparation example provides a method for preparing Phyllanthus emblica extract, the preparation method comprising:

[0049] (1) The moisture content of Phyllanthus emblica was adjusted to 25 wt%, and steam explosion treatment was carried out at 0.8 MPa and 180 ℃ for 60 s, with a pressure release rate of <0.1 seconds. The explosion was repeated once, and the product was dried to a moisture content of 8 wt%. The product was then pulverized to a mesh size of no more than 80 mesh to obtain the pretreated Phyllanthus emblica product.

[0050] (2) Mix the pretreated amla product with water at a ratio of 1 g: 15 mL, heat and reflux for 45 min, extract twice, pass through a 600 mesh filter cloth and a 5 μm filter bag to obtain the extract; concentrate the extract to a solid content of 10 wt%, refrigerate at 4 ℃ for 1 h for flocculation, filter, sterilize the filtrate, and dry to obtain the final product.

[0051] Preparation Example 3

[0052] This preparation example provides a method for preparing a prickly pear extract, the method comprising:

[0053] (1) Dry the prickly pear directly to a moisture content of 8 wt%, and pulverize it to a mesh size not exceeding 80 to obtain the pretreated prickly pear product;

[0054] (2) Mix the pretreated prickly pear product with water at a ratio of 1 g: 15 mL, heat and reflux for 45 min, extract twice, pass through a 600 mesh filter cloth and a 5 μm filter bag to obtain the extract; concentrate the extract to a solid content of 10 wt%, refrigerate at 4 ℃ for 1 h for flocculation, filter, sterilize the filtrate, and dry to obtain the final product.

[0055] Example 1

[0056] This embodiment provides a composition with anti-inflammatory and anti-allergic effects. The raw materials for preparing the composition include 10 parts by weight of Boletus edulis, 10 parts by weight of Phyllanthus emblica, and 6 parts by weight of Prickly pear.

[0057] Its preparation methods include:

[0058] (1) Adjust the moisture content of Boletus edulis to 20 wt%, cut it into 2-5 cm particles, and steam explosion treatment at 0.6 MPa and 165 ℃ for 40 s, with a pressure release rate of <0.1 seconds. Circulate the explosion once, dry it to a moisture content of 4 wt%, and pulverize it to no more than 80 mesh to obtain the pretreated Boletus edulis product.

[0059] (2) The moisture content of Phyllanthus emblica was adjusted to 25 wt%, and steam explosion treatment was carried out at 0.8 MPa and 180 ℃ for 60 s, with a pressure release rate of <0.1 seconds. The explosion was repeated once, and the product was dried to a moisture content of 8 wt%. The product was then pulverized to a mesh size of no more than 80 mesh to obtain the pretreated Phyllanthus emblica product.

[0060] (3) Dry the prickly pear directly to a moisture content of 8 wt%, and pulverize it to a mesh size not exceeding 80 to obtain the pretreated prickly pear product;

[0061] (4) Mix the pretreated Boletus product, the pretreated Phyllanthus product, the pretreated Prickly Pear product and water, with a material-to-liquid ratio of 1 g:15 mL, heat and reflux for 45 min, extract twice, and pass through a 600-mesh filter cloth and a 5 μm filter bag to obtain the extract.

[0062] (5) Concentrate the extract to a solid content of 10 wt%, refrigerate at 4 ℃ for 1 h for flocculation, filter, sterilize the filtrate, and dry to obtain the final product.

[0063] Example 2

[0064] This embodiment provides a composition with anti-inflammatory and anti-allergic effects. The raw materials for preparing the composition include 5 parts by weight of Boletus edulis, 20 parts by weight of Phyllanthus emblica, and 10 parts by weight of Prickly pear.

[0065] Its preparation methods include:

[0066] (1) Adjust the moisture content of Boletus edulis to 30 wt%, cut it into 2-5 cm particles, and steam explosion treatment at 0.4 MPa and 140 ℃ for 60 s, with a pressure release rate of <0.1 seconds. Circulate the explosion once, dry it to a moisture content of 2 wt%, and pulverize it to no more than 80 mesh to obtain the pretreated Boletus edulis product.

[0067] (2) The moisture content of Phyllanthus emblica was adjusted to 35 wt%, and steam explosion treatment was carried out at 0.5 MPa and 155 ℃ for 100 s, with a pressure release rate of <0.1 seconds. The explosion was repeated once, and the product was dried to a moisture content of 5 wt%. The product was then pulverized to a mesh size of no more than 80 mesh to obtain the pretreated Phyllanthus emblica product.

[0068] (3) Dry the prickly pear directly to a moisture content of 5 wt%, and pulverize it to a mesh size not exceeding 80 to obtain the pretreated prickly pear product;

[0069] (4) Mix the pretreated Boletus product, the pretreated Phyllanthus product, the pretreated Prickly Pear product and water, with a material-to-liquid ratio of 1 g:10 mL, heat and reflux for 0.5 h, extract 3 times, and pass through a 600-mesh filter cloth and a 5 μm filter bag to obtain the extract;

[0070] (5) Concentrate the extract to a solid content of 8 wt%, refrigerate at 2 ℃ for 1.5 h for flocculation, filter, sterilize the filtrate, and dry to obtain the final product.

[0071] Example 3

[0072] This embodiment provides a composition with anti-inflammatory and anti-allergic effects. The raw materials for preparing the composition include 20 parts by weight of Boletus edulis, 5 parts by weight of Phyllanthus emblica, and 2 parts by weight of Prickly pear.

[0073] Its preparation methods include:

[0074] (1) Adjust the moisture content of Boletus edulis to 25 wt%, cut it into 2-5 cm particles, and steam explosion treatment at 0.8 MPa and 180 ℃ for 30 s, with a pressure release rate of <0.1 seconds. Circulate the explosion once, dry it to a moisture content of 6 wt%, and pulverize it to no more than 80 mesh to obtain the pretreated Boletus edulis product.

[0075] (2) The moisture content of Phyllanthus emblica was adjusted to 30 wt%, and steam explosion treatment was carried out at 0.6 MPa and 165 ℃ for 80 s, with a pressure release rate of <0.1 seconds. The explosion was repeated once, and the product was dried to a moisture content of 10 wt%. The product was then pulverized to a mesh size of no more than 80 mesh to obtain the pretreated Phyllanthus emblica product.

[0076] (3) Dry the prickly pear directly to a moisture content of 10 wt%, and pulverize it to a mesh size not exceeding 80 to obtain the pretreated prickly pear product;

[0077] (4) Mix the pretreated Boletus product, the pretreated Phyllanthus product, the pretreated Prickly Pear product and water, with a material-to-liquid ratio of 1 g: 20 mL, heat and reflux for 1 h, extract once, and pass through a 600-mesh filter cloth and a 5 μm filter bag to obtain the extract;

[0078] (5) Concentrate the extract to a solid content of 12 wt%, refrigerate at 6 ℃ for 0.8 h for flocculation, filter, sterilize the filtrate, and dry to obtain the final product.

[0079] Example 4

[0080] This embodiment provides a composition with anti-inflammatory and anti-allergic effects, which differs from Example 1 only in the preparation method. The preparation method step (1) is as follows: "Adjust the moisture content of Boletus edulis to 10 wt%, cut it into 2-5 cm particles, perform steam explosion treatment at 0.6 MPa and 165 ℃ for 40 s, with a pressure release rate of <0.1 seconds, cycle the explosion once, dry it to a moisture content of 4 wt%, and pulverize it to no more than 80 mesh to obtain the pretreated Boletus edulis product", and other operations remain unchanged.

[0081] Example 5

[0082] This embodiment provides a composition with anti-inflammatory and anti-allergic effects, which differs from Example 1 only in the preparation method. The preparation method step (1) is as follows: "Adjust the moisture content of Boletus edulis to 40 wt%, cut it into 2-5 cm particles, perform steam explosion treatment at 0.6 MPa and 165 ℃ for 40 s, with a pressure release rate of <0.1 seconds, cycle the explosion once, dry it to a moisture content of 4 wt%, and pulverize it to no more than 80 mesh to obtain the pretreated Boletus edulis product", and other operations remain unchanged.

[0083] Example 6

[0084] This embodiment provides a composition with anti-inflammatory and anti-allergic effects, which differs from Example 1 only in the preparation method. The preparation method step (1) is as follows: "Adjust the moisture content of Boletus edulis to 20 wt%, cut it into 2-5 cm particles, perform steam explosion treatment at 0.2 MPa and 130 ℃ for 100 s, release pressure rate <0.1 seconds, cycle the explosion once, dry it to a moisture content of 4 wt%, and pulverize it to no more than 80 mesh to obtain the pretreated Boletus edulis product", and other operations remain unchanged.

[0085] Example 7

[0086] This embodiment provides a composition with anti-inflammatory and anti-allergic effects, which differs from Example 1 only in the preparation method. The preparation method step (1) is as follows: "Adjust the moisture content of Boletus edulis to 20 wt%, cut it into 2-5 cm particles, perform steam explosion treatment at 1.0 MPa and 190 ℃ for 20 s, with a pressure release rate of <0.1 seconds, cycle the explosion once, dry it to a moisture content of 4 wt%, and pulverize it to no more than 80 mesh to obtain the pretreated Boletus edulis product", and other operations remain unchanged.

[0087] Example 8

[0088] This embodiment provides a composition with anti-inflammatory and anti-allergic effects, which differs from Example 1 only in the preparation method. The preparation method step (1) is as follows: "Adjust the moisture content of Boletus edulis to 20 wt%, cut it into 2-5 cm particles, perform pressure treatment at 0.2 MPa and 130 ℃ for 40 s, dry it to a moisture content of 4 wt%, and pulverize it to no more than 80 mesh to obtain the pretreated Boletus edulis product", and other operations remain unchanged.

[0089] Example 9

[0090] This embodiment provides a composition with anti-inflammatory and anti-allergic effects, which differs from Example 1 only in the preparation method. Step (1) of the preparation method is "drying Boletus edulis to a moisture content of 4 wt%, pulverizing to no more than 80 mesh to obtain a pretreated Boletus edulis product", while other operations remain unchanged.

[0091] Example 10

[0092] This embodiment provides a composition with anti-inflammatory and anti-allergic effects, which differs from Example 1 only in the preparation method. Step (2) of the preparation method is as follows: "Adjust the moisture content of Phyllanthus emblica to 15 wt%, perform steam explosion treatment at 0.8 MPa and 180°C for 60 s, with a pressure release rate of <0.1 seconds, cycle the explosion once, dry to a moisture content of 8 wt%, and pulverize to no more than 80 mesh to obtain the pretreated Phyllanthus emblica product", with other operations remaining unchanged.

[0093] Example 11

[0094] This embodiment provides a composition with anti-inflammatory and anti-allergic effects, which differs from Example 1 only in the preparation method. Step (2) of the preparation method is as follows: "Adjust the moisture content of Phyllanthus emblica to 45 wt%, perform steam explosion treatment at 0.8 MPa and 180°C for 60 s, with a pressure release rate of <0.1 seconds, cycle the explosion once, dry to a moisture content of 8 wt%, and pulverize to no more than 80 mesh to obtain the pretreated Phyllanthus emblica product", with other operations remaining unchanged.

[0095] Example 12

[0096] This embodiment provides a composition with anti-inflammatory and anti-allergic effects, which differs from Example 1 only in the preparation method. Step (2) of the preparation method is as follows: "Adjust the moisture content of Phyllanthus emblica to 25 wt%, perform steam explosion treatment at 0.2 MPa and 130°C for 120 s, with a pressure release rate of <0.1 seconds, cycle the explosion once, dry to a moisture content of 8 wt%, and pulverize to no more than 80 mesh to obtain the pretreated Phyllanthus emblica product", with other operations remaining unchanged.

[0097] Example 13

[0098] This embodiment provides a composition with anti-inflammatory and anti-allergic effects, which differs from Example 1 only in the preparation method. Step (2) of the preparation method is as follows: "Adjust the moisture content of Phyllanthus emblica to 25 wt%, perform steam explosion treatment at 1.2 MPa and 200°C for 30 s, with a pressure release rate of <0.1 seconds, cycle the explosion once, dry to a moisture content of 8 wt%, and pulverize to no more than 80 mesh to obtain the pretreated Phyllanthus emblica product", with other operations remaining unchanged.

[0099] Example 14

[0100] This embodiment provides a composition with anti-inflammatory and anti-allergic effects, which differs from Example 1 only in the preparation method. Step (2) of the preparation method is to "adjust the moisture content of Phyllanthus emblica to 25 wt%, perform pressure treatment at 0.2 MPa and 130°C for 60 s, dry to a moisture content of 8 wt%, and pulverize to no more than 80 mesh to obtain the pretreated Phyllanthus emblica product", with other operations remaining unchanged.

[0101] Example 15

[0102] This embodiment provides a composition with anti-inflammatory and anti-allergic effects, which differs from Example 1 only in the preparation method. Step (2) of the preparation method is "drying Phyllanthus emblica to a moisture content of 8 wt%, pulverizing to no more than 80 mesh to obtain a pretreated Phyllanthus emblica product", while other operations remain unchanged.

[0103] Example 16

[0104] This embodiment provides a composition with anti-inflammatory and anti-allergic effects, which differs from Example 1 only in the preparation method. Step (5) of the preparation method is "concentrate the extract to a solid content of 10 wt%, filter, sterilize the filtrate, and dry to obtain the final product", while other operations remain unchanged.

[0105] Example 17

[0106] This embodiment provides a composition with anti-inflammatory and anti-allergic effects, which differs from Example 1 only in the preparation method.

[0107] Its preparation method is as follows:

[0108] (1) Adjust the moisture content of Boletus edulis to 20 wt%, cut it into 2-5 cm particles, and steam explosion treatment at 0.6 MPa and 165 ℃ for 40 s, with a pressure release rate of <0.1 seconds. Circulate the explosion once, dry it to a moisture content of 4 wt%, and pulverize it to no more than 80 mesh to obtain the pretreated Boletus edulis product.

[0109] (2) The moisture content of Phyllanthus emblica was adjusted to 25 wt%, and steam explosion treatment was carried out at 0.8 MPa and 180 ℃ for 60 s, with a pressure release rate of <0.1 seconds. The explosion was repeated once, and the product was dried to a moisture content of 8 wt%. The product was then pulverized to a mesh size of no more than 80 mesh to obtain the pretreated Phyllanthus emblica product.

[0110] (3) Dry the prickly pear to a moisture content of 8 wt%, and pulverize it to a mesh size not exceeding 80 to obtain the pretreated prickly pear product;

[0111] (4) The pretreated Boletus product, the pretreated Phyllanthus product, and the pretreated Prickly Pear product were mixed with water at a ratio of 1 g: 15 mL. The mixture was heated and refluxed for 45 min, and extracted twice. The extracts were then passed through a 600-mesh filter cloth and a 5 μm filter bag to obtain Boletus extract, Phyllanthus extract, and Prickly Pear extract.

[0112] (5) The extracts of Boletus edulis, Phyllanthus emblica and Prickly pear were concentrated to a solid content of 10 wt%, flocculated at 4 ℃ for 1 h, filtered, sterilized and dried to obtain Boletus edulis extract, Phyllanthus emblica extract and Prickly pear extract.

[0113] (6) Mix the Boletus extract, Phyllanthus emblica extract and Prickly pear extract to obtain the final product.

[0114] Comparative Example 1

[0115] This comparative example provides a composition with anti-inflammatory and anti-allergic effects, which differs from Example 1 only in that it does not contain Boletus, and its reduced mass is proportionally allocated to the mass of Phyllanthus emblica and Prickly pear.

[0116] The preparation method is the same as in Example 1.

[0117] Comparative Example 2

[0118] This comparative example provides a composition with anti-inflammatory and anti-allergic effects, which differs from Example 1 only in that it does not contain Phyllanthus emblica, and its reduced mass is proportionally allocated to the mass of Boletus edulis and Prickly pear.

[0119] The preparation method is the same as in Example 1.

[0120] Comparative Example 3

[0121] This comparative example provides a composition with anti-inflammatory and anti-allergic effects, which differs from Example 1 only in that it does not contain prickly pear, and its reduced mass is proportionally allocated to the mass of porcini and amla.

[0122] The preparation method is the same as in Example 1.

[0123] Test Example 1

[0124] Hyaluronidase inhibition effect test

[0125] Prepare a 0.25 mg / mL hyaluronidase (HAS) solution and a 1 mg / mL sodium hyaluronate solution using 0.1 mM acetate buffer. Prepare a 1 mg / mL tannic acid and sample solution using water as the solvent. Mix 500 μL of HAS solution and 500 μL of sample solution thoroughly, set up a control blank, and incubate at 37 ℃ for 20 min. Add 100 μL of 12.5 mM calcium chloride solution to activate HAS and continue the reaction for another 20 min. Then add 500 μL of hyaluronidase solution, replacing the control blank with 0.1 mM acetate buffer, and incubate at 37 ℃ for 40 min. Stop the reaction by adding 0.4 M sodium hydroxide solution and 0.4 M potassium borate solution sequentially. Then mix 100 μL of the reaction solution with 100 μL of dimethylaminobenzaldehyde colorimetric reagent and measure the absorbance at 37 ℃ and 585 nm.

[0126]

[0127] A—Absorbance value of the sample solution

[0128] B—Absorbance value of the blank sample

[0129] C—Absorbance value of the control solution

[0130] D—Absorbance value of the control blank

[0131] The results of the hyaluronidase inhibition rate (%) are shown in Table 1.

[0132] Table 1

[0133]

[0134] As shown in Table 1, the composition described in this application has excellent hyaluronidase inhibition effect. The pretreatment process of Boletus edulis and Phyllanthus emblica affects the efficacy of the composition. The composition prepared by mixed extraction of Boletus edulis, Phyllanthus emblica and Prickly pear has a certain synergistic effect on the above-mentioned efficacy. The absence of any one of them will affect the efficacy of the composition.

[0135] Test Example 2

[0136] Collagenase inhibition effect test

[0137] Prepare 1 mg / mL tetracycline hydrochloride (positive control) and 2 mg / mL test sample solutions using Tris-HCl buffer (pH = 7.5 ± 0.2, containing 5 mmol / mL CaCl2). Set up blank, control, sample, and sample blank groups. Add 60 μL of test sample solution to a 96-well plate. Replace the test sample solution with 60 μL of buffer solution in the blank group. Add 140 μL of 0.2 mg / mL collagenase solution to the control and sample groups. Replace the blank and sample blank groups with the same volume of buffer solution. After incubating at 37 ℃ for 20 min, add 40 μL of 0.5 mg / mL FALGPA buffer solution. After incubating at 37 ℃ for 20 min, measure the absorbance of each component at 330 nm. Calculate the collagenase inhibition rate using the following formula:

[0138] Sample blank group: 60 μL sample solution + 140 μL buffer + 40 μL substrate

[0139] Sample group: 60 μL sample solution + 140 μL collagenase + 40 μL substrate

[0140] Control group: 60 μL buffer + 140 μL collagenase + 40 μL substrate

[0141] Control group: 200 μL buffer + 40 μL substrate

[0142]

[0143] Each group was measured three times on average, and the average value was taken to calculate the collagenase inhibition rate. The results are shown in Table 2.

[0144] Table 2

[0145]

[0146] As shown in Table 2, the composition described in this application has excellent collagenase inhibition effect. The pretreatment process of Boletus edulis and Phyllanthus emblica affects the efficacy of the composition. The composition prepared by mixed extraction of Boletus edulis, Phyllanthus emblica and Prickly pear has a certain synergistic effect on the above-mentioned efficacy. The absence of any one of them will affect the efficacy of the composition.

[0147] Test Example 3

[0148] Tyrosinase inhibition effect test

[0149] A 0.5 mg / mL L-tyrosine solution and a 100 μg / mL tyrosinase solution were prepared using phosphate buffer (pH 6.8) as the solvent. A 1 mg / mL arbutin (positive control) solution and the sample solution were prepared using water as the solvent. The tyrosine solution, tyrosinase solution, and sample solution were mixed thoroughly. A blank control group was set up. The mixture was incubated at 37 °C for 30 min, and the absorbance was measured at 475 nm. The inhibition rate of tyrosinase activity by the sample was calculated using the following formula:

[0150]

[0151] Sample group: 100 μL tyrosine solution + 50 μL sample solution + 50 μL tyrosinase solution

[0152] Sample blank group: 100 μL tyrosine solution + 50 μL sample solution + 50 μL buffer

[0153] Control group: 100 μL tyrosine solution + 50 μL tyrosinase + 50 μL buffer solution

[0154] Control group: 100 μL tyrosine solution + 100 μL buffer solution

[0155] Each group was measured an average of 3 times, and the average value was taken to calculate the tyrosinase inhibition rate. The results are shown in Table 3.

[0156] Table 3

[0157]

[0158] As shown in Table 3, the composition described in this application has excellent tyrosinase inhibition effect. The pretreatment process of Boletus edulis and Phyllanthus emblica affects the efficacy of the composition. The composition prepared by mixed extraction of Boletus edulis, Phyllanthus emblica and Prickly pear has a certain synergistic effect on the above-mentioned efficacy. The absence of any one of them will affect the efficacy of the composition.

[0159] Test Example 4

[0160] α-Amylase Inhibition Effect Test

[0161] Take 250 μL of phosphate buffer (pH 6.8), add 50 μL of the test sample (1 mg / mL) and 50 μL of α-amylase solution (1 unit / mL), shake well, mix at 37 °C and incubate in a water bath for 10 min. Add 200 μL of 1% soluble starch solution, mix at 37 °C and incubate in a water bath for another 10 min. Add 200 μL of 3,5-dinitrosalicylic acid (DNS reagent), mix well, incubate in a water bath at 80 °C for 10 min, then rapidly cool. Observe until the control group shows obvious color change, then stop. Use phosphate buffer instead of enzyme solution as a blank control and acarbose as a positive control. Each experiment is repeated 3 times. Measure the absorbance at 540 nm, and take the average absorbance of each sample from multiple wells to calculate the α-amylase inhibition rate of each test sample.

[0162]

[0163] Each group was measured 3 times on average, and the average value was taken. The results of the α-amylase inhibition rate (%) are shown in Table 4.

[0164] Table 4

[0165]

[0166] As shown in Table 4, the composition described in this application has excellent α-amylase inhibition effect. The pretreatment process of Boletus edulis and Phyllanthus emblica affects the efficacy of the composition. The composition prepared by mixed extraction of Boletus edulis, Phyllanthus emblica and Prickly pear has a certain synergistic effect on the above-mentioned efficacy. The absence of any one of them will affect the efficacy of the composition.

[0167] Test Example 5

[0168] Anti-inflammatory effect test

[0169] RAW264.7 cells in logarithmic growth phase with good morphology were selected and prepared into 5×10⁶ cells using complete culture medium. 4 Cell suspension of 1000 μL / mL was seeded into 24-well plates at a rate of 5 × 10⁶ cells / mL. 4 Each sample was incubated at 37 °C in a 5% CO2 incubator for 24 h.

[0170] Drug administration and LPS: Blank control group, model control group, positive control group and sample group were set up.

[0171] Blank control group: Add complete culture medium to each well.

[0172] Positive control group: 5 μM dexamethasone acetate (DEX) diluted with complete culture medium was added to each well.

[0173] Negative control group: LPS with a final concentration of 1 μg / mL

[0174] Sample group: Add the test sample (concentration of 250 μg / mL) diluted with complete culture medium to each well.

[0175] Each group was set up with 3 replicates and incubated in a 37 ℃, 5% CO2 incubator for 2 h. Except for the blank control group, LPS was added to each well to a final concentration of 1 μg / mL and incubated again in the incubator for 24 h.

[0176] Inflammatory factor detection: The NO content in the collected cell supernatant was detected according to the instructions of the NO detection kit. The experimental results were analyzed using data processing software, and the results are shown in Table 5.

[0177] Table 5

[0178]

[0179] Note: ### This indicates that compared with the blank control group, p < 0.001; *** This indicates a comparison with the model control group, p < 0.001.

[0180] As shown in Table 5, LPS stimulation significantly increased the relative expression of NO (p<0.001), indicating the successful construction of the inflammation model. Compared with the model control group, the test sample treatment significantly reduced the relative expression of NO (p<0.05), and the effects of Examples 1-3 were at a high level. These results indicate that the combination of Boletus edulis, Phyllanthus emblica, and Rosa rugosa has a strong anti-inflammatory effect and a certain synergistic effect; the absence of any one of these components will affect the efficacy of the combination.

[0181] Test Example 6

[0182] Allergy test

[0183] Wild-type AB strain zebrafish with a 3-day pf growth rate (dpf) were randomly selected and treated in 24-well plates, with 10 zebrafish per well. The concentration of the test sample was 250 μg / mL, and the positive control sodium cromoglycate was 1000 μg / mL. Normal control and model control groups were also set up, with 1 mL per well, and the experiment was repeated in triplicate. Except for the normal control group, all experimental groups were given compound C48 / 80 in water to establish a zebrafish allergy model. After 24 h of co-treatment with BAPNA, the liquid was transferred to 96-well plates, 10 zebrafish / 250 μL / well, and the OD of tryptase expression level in each experimental group was measured. 405 value.

[0184]

[0185] Sample group: Average absorbance of each sample group

[0186] Normal control group: The average absorbance of the normal control group that did not receive compound C48 / 80.

[0187] Sample blank group: absorbance values ​​of sample blanks of equal volume.

[0188] Normal blank group: absorbance value of the same volume of embryo culture medium

[0189] Statistical results are expressed as mean ± SE. Results are as follows: Figure 1 As shown.

[0190] Depend on Figure 1 It is known that the composition described in this application has excellent anti-allergic effects and can significantly reduce the expression level of trypsin-like enzymes, with better effects than Boletus extract, Phyllanthus emblica extract and Rosa rugosa extract.

[0191] The applicant declares that the present invention is illustrated by the above embodiments to demonstrate a composition with anti-inflammatory and anti-allergic effects and its application, but the present invention is not limited to the above embodiments, that is, it does not mean that the present invention must rely on the above embodiments to be implemented. Those skilled in the art should understand that any improvements to the present invention, equivalent substitutions of the raw materials of the product of the present invention, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.

[0192] The preferred embodiments of the present invention have been described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the scope of the technical concept of the present invention, various simple modifications can be made to the technical solution of the present invention, and these simple modifications all fall within the protection scope of the present invention.

[0193] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not describe the various possible combinations separately.

Claims

1. A composition having anti-inflammatory and anti-allergic effects, characterized in that, The raw materials for preparing the composition include Boletus edulis, Phyllanthus emblica, and Prickly pear.

2. The composition with anti-inflammatory and anti-allergic effects according to claim 1, characterized in that, The raw materials for preparing the composition include, by mass parts, 5-20 parts of Boletus edulis, 5-20 parts of Phyllanthus emblica, and 2-10 parts of Prickly pear.

3. The composition with anti-inflammatory and anti-allergic effects according to claim 1, characterized in that, The composition is prepared by a method comprising the following steps: (1) Boletus edulis and Phyllanthus emblica were pretreated by steam explosion. (2) Mix the pretreated Boletus, the pretreated Phyllanthus emblica, and Prickly pear with water, and reflux to extract the extract. (3) Filter and concentrate the extract to obtain a concentrated solution. Refrigerate the concentrated solution to flocculate it, filter it, and then dry it to obtain the final product.

4. The composition with anti-inflammatory and anti-allergic effects according to claim 3, characterized in that, The water content of the Boletus edulis is 20%-30 wt%, and the water content of Phyllanthus emblica is 25-35 wt%.

5. The composition with anti-inflammatory and anti-allergic effects according to claim 3, characterized in that, The steam explosion of the bolete was performed at a pressure of 0.4-0.8 MPa, a temperature of 140-180 ℃, and a time of 30-60 s.

6. The composition with anti-inflammatory and anti-allergic effects according to claim 3, characterized in that, The pressure of the steam explosion of Phyllanthus emblica is 0.5-0.8 MPa, the temperature is 150-180 ℃, and the time is 60-100 s.

7. The composition with anti-inflammatory and anti-allergic effects according to claim 3, characterized in that, The reflux extraction time is 0.5-1 h, and the number of times is 1-3.

8. The composition with anti-inflammatory and anti-allergic effects according to claim 3, characterized in that, The material-to-liquid ratio for the reflux extraction is 1 g:(10-20) mL.

9. The composition with anti-inflammatory and anti-allergic effects according to claim 3, characterized in that, The refrigerated flocculation temperature is 2-6 ℃, and the time is 0.5-2 h.

10. The use of a composition having anti-inflammatory and anti-allergic effects according to any one of claims 1-9 in the preparation of a product having anti-inflammatory and anti-allergic effects.

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