Application of broad-leaved epiphyllum extract in preparation of perfume

Abstract

The invention discloses an application of a broad-leaved epiphyllum extract in preparation of perfume, and relates to the technical field of broad-leaved epiphyllum application, the broad-leaved epiphyllum extract is composed of a broad-leaved epiphyllum extract (1), a broad-leaved epiphyllum extract (2), a broad-leaved epiphyllum extract (3) and a broad-leaved epiphyllum extract (4), the broad-leaved epiphyllum extract (1) is an extract obtained by adopting an ethanol low-temperature extraction method; the broad-leaved epiphyllum extract (2) is an extract obtained by adopting a supercritical CO2 extraction method; the broad-leaved epiphyllum extract (3) is an extract obtained by adopting a grease extraction method; the broad-leaved epiphyllum extract (4) is an extract obtained by adopting a steam distillation method; the mass part ratio of the broad-leaved epiphyllum extract (1) to the broad-leaved epiphyllum extract (2) to the broad-leaved epiphyllum extract (3) to the broad-leaved epiphyllum extract (4) is (10-25): (70-85): (2-8): (0.5-3); the broad-leaved epiphyllum extract and acceptable auxiliary materials for preparing the perfume are proportioned and uniformly mixed to prepare the perfume, and the prepared perfume has the fresh and clear appearance of the broad-leaved epiphyllum when the broad-leaved epiphyllum is in the beginning of blooming, the rich sweet aroma of the middle section and the elegant aftertaste of the tail section, and is free of burnt flavor, sour flavor, alcohol residual flavor and other foreign flavors.

Description

Technical Field

[0001] This invention relates to the field of epiphyllum application technology, specifically to the application of epiphyllum extract in the preparation of perfume. Background Technology

[0002] Epiphyllum ( Epiphyllum oxypetalum *Epiphyllum oxypetalum* (DC.) Haw. is a perennial succulent plant belonging to the Cactaceae family, native to Mexico and Nicaragua. Due to its short-lived nocturnal blooming and unique visual and olfactory value, it is known as the "Queen of the Night." Not only does it have high ornamental value, but it is also a plant with both medicinal and edible uses. Traditionally, it has been used to treat gastrointestinal ailments. Modern research has further confirmed that its extracts contain moisturizing, whitening, and antioxidant active ingredients, and it has already begun to be used in the cosmetics industry. However, one of the most core values ​​of *Epiphyllum oxypetalum*—its sweet and rich fragrance and the utilization of its volatile organic compounds (VOCs)—has not yet been fully developed, and related technologies have significant shortcomings.

[0003] Current research on the aroma components of Epiphyllum oxypetalum reveals insufficient understanding of the spatiotemporal release patterns of its VOCs. Early studies largely focused on the pharmacological activities and genomic characteristics of Epiphyllum oxypetalum, with very limited exploration of the dynamic changes in its fragrance. Although some studies have attempted to analyze VOCs in Epiphyllum oxypetalum using gas chromatography-related techniques, there are significant technical limitations. For example, while headspace gas chromatography-ion mobility spectrometry (HS-GC-IMS) can quickly obtain compound fingerprints, it cannot distinguish the differences in VOCs at different flowering stages (pre-flowering, semi-open, and full bloom) due to insufficient database coverage and sample pretreatment (such as mechanical damage caused by 40℃ incubation and petal separation). Furthermore, it is prone to detecting C6 green leaf volatiles induced by heat or mechanical damage (such as (E)-2-hexenal), which deviates from the aroma composition of Epiphyllum oxypetalum in its natural state. While headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) can detect more VOCs, it requires mechanical separation of flower organs and liquid nitrogen freezing-thawing treatment, resulting in an excessively high proportion of fatty acid derivatives in the detection results. This masks the true characteristic of Epiphyllum oxypetalum's VOCs being mainly terpenoid compounds in its natural state, making it impossible to accurately identify the source and release pattern of key aroma components.

[0004] It wasn't until the application of dynamic headspace collection-gas chromatography-mass spectrometry (DHC-GC-MS) technology that the spatiotemporal release characteristics of VOCs from Epiphyllum oxypetalum were clearly identified for the first time: the total release of VOCs from Epiphyllum oxypetalum begins to increase significantly around 21:00 at night, reaching its peak around 23:00, with terpenoids accounting for as much as 54.5%, which are the core components of the aroma; at the same time, five differentially expressed VOCs that play a key role in the aroma of Epiphyllum oxypetalum were identified—geraniol (accounting for the highest proportion of total VOCs at 76.13%, with a sweet rose aroma), methyl salicylate (wintergreen scent), citral (lemon scent), benzyl alcohol (light fruity scent), and benzyl valerate (floral and fruity scent), and these key components mainly originate from the petals (VOCs released from the petals account for 49.4% of the total). However, this study only stays at the level of component analysis and has not translated this scientific discovery into industrialized extract preparation and application technology, resulting in a serious disconnect between the current Epiphyllum oxypetalum extract preparation process and the natural laws of aroma components.

[0005] Regarding the preparation technology of Epiphyllum oxypetalum extract, existing methods have three major problems: First, the raw material collection lacks scientific basis. Harvesting time and parts are not determined based on the spatiotemporal patterns of VOCs release, often arbitrarily selecting Epiphyllum oxypetalum during the day or at non-fully blooming stages, and potentially collecting non-primary aroma-producing organs such as stamens and pistils, resulting in extremely low levels of key components such as geraniol and methyl salicylate in the extract; at the same time, the influence of the harvesting environment is ignored (such as harvesting on rainy days or at night with high humidity, leading to excessively high water content in the flowers), further reducing the quality of the raw materials. Second, the extraction process is unreasonable. Existing methods fail to consider the volatile and high-temperature-destructive characteristics of Epiphyllum oxypetalum's VOCs (mainly terpenes), or use excessively high extraction temperatures (e.g., exceeding 40°C) leading to aroma component loss, or use inappropriate solvents (e.g., organic solvents with off-flavors) introducing odors. Furthermore, they lack optimized extraction parameters (e.g., supercritical CO2 extraction pressure, temperature, flow rate) for different pre-treated raw materials (fresh blocks, freeze-dried powder, fine strands, etc.), resulting in low extract purity, poor aroma reproduction, and an inability to retain the complete aroma profile of Epiphyllum oxypetalum—from its initial fresh and clear bloom to its rich and sweet mid-scent and its delicate lingering aftertaste. Thirdly, resource utilization is low. In the traditional preparation of dried Epiphyllum oxypetalum flowers, a large amount of flavor substances (including key VOCs) lost with water evaporation are directly discarded without recycling, resulting in resource waste and missing out on a high-value-added aroma base.

[0006] In summary, there is an urgent need in the current technological field to develop new applications for Epiphyllum oxypetalum, promote its extension from ornamental and medicinal uses to industries such as high-value-added fragrance bases, and fill the technological gap in the fragrance utilization of Epiphyllum oxypetalum. Summary of the Invention

[0007] The purpose of this invention is to solve the above-mentioned technical problems and propose an application of Epiphyllum extract in the preparation of perfumes, which produces perfumes with high fragrance fidelity and good product stability.

[0008] This invention is achieved through the following technical solution: An application of Epiphyllum extract in the preparation of perfume, wherein the Epiphyllum extract is composed of Epiphyllum extract ①, Epiphyllum extract ②, Epiphyllum extract ③, and Epiphyllum extract ④. The Epiphyllum extract ① is an extract obtained by low-temperature ethanol extraction, and contains geraniol ≥ 62.5%, benzyl alcohol ≥ 9.8%, and linalool ≥ 1.06% in Epiphyllum extract ①. The Epiphyllum extract ② is an extract obtained by supercritical CO2 extraction. The Epiphyllum extract ② contains geraniol ≥75%, methyl salicylate ≥4.3%, citral ≥2.23%, and benzyl alcohol ≥10%. The Epiphyllum extract ③ is an extract obtained by oil extraction, and contains geraniol ≥ 66.5%, ocimene ≥ 6.35%, farnesene ≥ 2.3%, and pinene ≥ 1.25%. The Epiphyllum extract ④ is an extract obtained by steam distillation, and contains geraniol ≥ 58%, methyl salicylate ≥ 4.3%, citral ≥ 2.23%, benzyl alcohol ≥ 10%, and benzyl salicylate ≥ 1.22%. The mass fraction ratio of Epiphyllum extract ①: Epiphyllum extract ②: Epiphyllum extract ③: Epiphyllum extract ④ is 10~25:70~85:2~8:0.5~3; The epiphyllum extract is mixed with perfume-grade excipients in an acceptable ratio, the excipients including at least one of solvents, stabilizers, and fragrance enhancers, and then the mixture is homogeneous to produce the perfume.

[0009] Furthermore, the epiphyllum extract① includes volatile aroma components and non-volatile components, wherein the volatile aroma components include: geraniol, benzyl alcohol, linalool, benzaldehyde and its glycosides; and the non-volatile components include: flavonoids, phenolic acids, polysaccharides, amino acids, pigments and trace waxes.

[0010] Furthermore, the epiphyllum extract② includes terpenes, esters, alcohols, aldehydes and ketones, volatile aroma compounds, and fat-soluble substances; Among them: terpenes include ocimene, farnesene, and caryophyllene; esters include benzyl acetate, methyl salicylate, benzyl salicylate, and benzyl benzoate; alcohols include geraniol, linalool, benzyl alcohol, and nerol; and aldehydes and ketones include citral, nonanal, and benzaldehyde.

[0011] Furthermore, the epiphyllum extract③ includes highly fat-soluble compounds, including sesquiterpenes, long-chain aliphatic hydrocarbons, wax esters, and fat-soluble aroma precursors.

[0012] Furthermore, the epiphyllum extract ④ includes water-soluble components and some volatile substances. The water-soluble substances include sugars, pectin, amino acids, water-soluble vitamins, and glycosides; the volatile substances include geraniol, geranialdehyde, nerol, benzaldehyde, melon aldehyde, methyl salicylate, citral, benzyl salicylate, benzyl benzoate, and nerol.

[0013] Furthermore, the steps for obtaining Epiphyllum extract① by ethanol extraction are as follows: a1. Raw material harvesting: Select epiphyllum flowers in full bloom and harvest the petals between 10 pm and 2 am. Use a sterilized sharp tool to cut the petals 1-2 cm away from the plant. After harvesting, place them in a single layer in a clean and soft container, avoiding rainy days or nights with high humidity. b1. Immediately after harvesting, transfer the flowers to the processing workshop, remove diseased and damaged perianth pieces, and then preserve them with liquid nitrogen or dry ice. After quickly rinsing the perianth pieces with deionized water, absorb the moisture and cut them into small square pieces of 0.5±0.8cm. c1. Mix Epiphyllum oxypetalum and food-grade anhydrous ethanol at a mass-to-volume ratio of 1:6 to 1:9, put them into a brown glass bottle with a sealed cap, and extract at 4 to 8°C for 24 to 28 hours. d1. After extraction, filter the residue through a 300-mesh sieve. The filtrate is then rotary evaporated at 35~40℃ and -0.09~-0.1MPa until it becomes viscous. After dilution, filter the filtrate through a 0.22~0.28μm microporous membrane and collect the filtrate, which is the Epiphyllum extract①.

[0014] Furthermore, the steps for obtaining Epiphyllum extract ② by ethanol extraction are as follows: a2. Raw material harvesting: Select epiphyllum flowers in full bloom and harvest the petals between 10 pm and 2 am. Use a sterilized sharp tool to cut the petals 1-2 cm away from the plant. After harvesting, place them in a single layer in a clean and soft container, avoiding rainy days or nights with high humidity. b2. Immediately after harvesting, transfer the flowers to the processing workshop, remove diseased and damaged perianth pieces, and then preserve them with liquid nitrogen or dry ice. After vacuum freeze-drying the perianth pieces at -50℃, pulverize them into 80-mesh powder. c2. The Epiphyllum powder from step a2 is extracted using supercritical CO2. The extraction parameters are set as follows: extraction pressure 30~35MPa, extraction temperature 35~39℃, CO2 flow rate 20~24L / h, and extraction time 2~2.5h. d2. Then, a two-stage separation method is used to remove impurities. The parameters for the first-stage separation are pressure 10~13MPa and temperature 40~43℃, and the parameters for the second-stage separation are pressure 5MPa and temperature 30~34℃. The separated extract is collected, which is the epiphyllum extract ②.

[0015] Furthermore, the steps for obtaining Epiphyllum extract ③ by ethanol extraction are as follows: a3. Raw material harvesting: Select epiphyllum flowers in full bloom and harvest the tepals from 10 pm to 2 am. Use a sterilized sharp tool to cut the flower stalk 1-2 cm away from the plant. After harvesting, place them in a single layer in a clean and soft container, avoiding rainy days or nights with high humidity. b3. Immediately after harvesting, transfer the flowers to the processing workshop, remove diseased and damaged perianth pieces, and then preserve them with liquid nitrogen or dry ice. Wash and drain the perianth pieces and tear them into thin strips. c3. Use odorless liquid paraffin or glycerin as a carrier, mix petals and carrier at a mass ratio of 1:4~5, put into a sealed glass jar, and let it stand for 7 days in a cool, dark place at 10~15℃ for extraction. d3. After extraction, filter through a 200-mesh sieve to remove petal residue and collect the extract, which is the epiphyllum extract ③.

[0016] Furthermore, the steps for obtaining Epiphyllum extract ④ by ethanol extraction are as follows: a4. Raw material harvesting: Select epiphyllum flowers in full bloom and harvest the tepals from 10 pm to 2 am. Use a sterilized sharp tool to cut the flower stalk 1-2 cm away from the plant. After harvesting, place them in a single layer in a clean and soft container, avoiding rainy days or nights with high humidity. b4. Immediately after harvesting, transfer the flowers to the processing workshop, remove any diseased or damaged perianth pieces, and then preserve them with liquid nitrogen or dry ice. Place the perianth pieces in a drying oven. c4. Dry the perianth pieces at 35~42℃ with forced air. Connect a condenser to the exhaust vent of the drying oven. After drying for 1~2 hours, start collecting the volatile flavor substances. Every 30~35 minutes, use a sterile pipette to transfer the collected extracts to a sterile container. d4. After collecting the extract, filter it immediately to remove impurities, then filter it through a 0.22~0.28μm microporous membrane, and then concentrate it under reduced pressure at 40~45℃ to obtain the concentrated solution, which is the epiphyllum extract ④.

[0017] Furthermore, the sensory indicators of the Epiphyllum extract are as follows: clear and transparent in appearance, free of suspended matter and sediment, colorless or slightly yellow in color, and with the unique fragrance of Epiphyllum and no off-odor.

[0018] Furthermore, the epiphyllum extract is prepared in any of the following ways: a) Add 0.02~0.23wt% vitamin E or rosemary extract, seal, and freeze at -18℃; b) Store in a dark, sealed glass bottle at a temperature below 20°C in a cool, dry place.

[0019] Furthermore, the formulation of perfume includes the following steps: (1) Raw material preparation: Prepare raw materials according to the formula. The raw materials include Epiphyllum extract and excipients. Epiphyllum extract accounts for 11.5~15.0% of the total mass of raw materials. The excipients include dialdehyde alcohol, nerol formate, orange extract, methyl salicylate, isoamyl acetate, geraniol, hexenal, heptanal, Epiphyllum extract, indole, phenylethanol, gardenia extract, vanillin, tuberose main agent, jasmine extract, peony main agent, and propylene glycol. (2) Mixed fragrance: First, dissolve Epiphyllum extract ④ in propylene glycol, with a concentration of 1.5~2wt%, to obtain a propylene glycol solution of Epiphyllum extract ④; then mix the remaining Epiphyllum extracts with the excipients in a set ratio and stir evenly. (3) Dissolving in solvent: Add the mixed fragrance to the dialdehyde alcohol and stir until completely dissolved; (4) Let stand and age: Let the dissolved mixture stand for 5 to 6 weeks; (5) Filtration: The aged mixture is finely filtered through a microporous membrane to remove impurities; (6) Bottle storage: Put the filtered perfume into a perfume bottle and seal it for storage.

[0020] Furthermore, the feature is that: during the mixing in step (2), the extracts of Epiphyllum oxypetalum, Epiphyllum oxypetalum extract, orange blossom extract, gardenia extract and jasmine extract are mixed evenly first, and then neroli ester formate, methyl salicylate, isoamyl acetate, geraniol, hexenal, heptanal, indole, phenylethanol, vanillin, tuberose main agent and peony main agent are added and stirred.

[0021] Furthermore, in step (3), the stirring speed is controlled at 50~80 r / min and the stirring time is 30~60 min to ensure that the fragrance is completely dissolved in the acetalized alcohol.

[0022] Furthermore, in step (4), the aging environment is a light-protected environment with a temperature of 20~25℃ and a relative humidity of 40~60%.

[0023] Furthermore, in step (5), the pore size of the microporous filter membrane is 0.22~0.28μm.

[0024] Compared with the prior art, the present invention has the following advantages and beneficial effects: I. In this invention, existing technologies mostly obtain epiphyllum extract through four methods: low-temperature ethanol extraction, supercritical CO2 extraction, oil extraction, and steam distillation. However, when these four extracts are used alone to prepare epiphyllum perfume essence, they all have obvious defects: Specifically, the epiphyllum extract ① obtained by low-temperature ethanol extraction has poor volatility and complex aroma components, which cannot highlight the unique fragrance of epiphyllum; the epiphyllum extract ② obtained by supercritical CO2 extraction is rich in high concentration of volatile aroma molecules, but the fragrance is "flat" and the aroma is too strong and pungent; the epiphyllum extract ③ obtained by oil extraction has a soft fragrance, but the sweetness is too prominent and deviates from the original flavor of epiphyllum; the epiphyllum extract ④ prepared by steam distillation has a weak fragrance intensity and is accompanied by a noticeable "boiled" smell, which is difficult to meet the requirements of high-end perfume for fragrance quality. To this end, the present invention innovatively blends four extracts in a specific fragrance ratio, and achieves "tailoring" of the scent by leveraging the synergistic effect between the components. The complementary advantages of different extracts mask their respective defects, thereby constructing a better fragrance system and ultimately preparing a high-end Epiphyllum perfume with stable quality. Its fragrance can fully present the natural characteristics of Epiphyllum: "fresh and clear at the beginning of bloom - rich and sweet fragrance in the middle - elegant aftertaste in the end".

[0025] II. In this invention, four extraction methods—low-temperature ethanol extraction, supercritical CO2 extraction, oil extraction, and steam distillation—were used to obtain four extracts: Epiphyllum extract ①, Epiphyllum extract ②, Epiphyllum extract ③, and Epiphyllum extract ④. After fully considering factors such as the intensity of the Epiphyllum aroma, volatility profile, solubility compatibility, and sensory synergy, the optimal ratio of the four extracts (①, ②, ③, and ④) for preparing the Epiphyllum perfume was determined. The proportions of these four extracts determine the structure, stability, and final aesthetic appeal of the perfume.

[0026] Thirdly, this invention solves the compatibility problem between different extracts: the base solvent of perfume is ethanol, which has excellent compatibility with Epiphyllum extract ①, Epiphyllum extract ②, and Epiphyllum extract ③. However, Epiphyllum extract ④ is water-soluble, and directly adding it to ethanol-based perfumes will cause turbidity, flocculation, or even layering, damaging the perfume's clear appearance and stability. It is necessary to premix it with a co-solvent (such as a "water-ethanol" co-solvent system) to ensure its complete dissolution in the final product.

[0027] IV. In this invention, the method can produce a perfume with the characteristics of "fresh and clear fragrance at the beginning of blooming of the night-blooming cereus - rich and sweet fragrance in the middle - elegant aftertaste" and stable quality. The night-blooming cereus extract ② is rich in a large number of top notes (such as monoterpenes, low molecular weight aldehydes, etc.), which evaporate the fastest and can be smelled immediately. The night-blooming cereus extract ① contains more body notes / middle notes (such as alcohols, esters) and base notes / base notes (such as sesquiterpenes, waxes), which evaporate slowly and are responsible for the core characteristics and long-lasting fragrance of the perfume. The night-blooming cereus extract ④ mainly provides a "watery" feel and a slight top note support.

[0028] V. In this invention, the overall scent is targeted and "tailored" by precisely controlling the proportion of the compound of Epiphyllum extracts (a compound of Epiphyllum extract ①, Epiphyllum extract ②, Epiphyllum extract ③, and Epiphyllum extract ④). At the same time, the aroma performance is optimized by leveraging the synergistic effect between different components, and potential defects are compensated by the advantageous characteristics of each raw material. The specific control logic is as follows: Epiphyllum extract ③ has a soft and sweet fragrance, which can effectively neutralize the sharp aroma that may exist in Epiphyllum extract ②, making the overall fragrance more rounded and harmonious; Epiphyllum extract ① contains a broad spectrum of aroma components, which can provide a rich aroma background for the perfume and significantly improve the thickness and complexity of the fragrance; the "steamed" smell that is prone to appear in Epiphyllum extract ④ can be masked and modified by the rich and fresh floral fragrance of Epiphyllum extract ②, ultimately achieving effective avoidance of aroma defects and improvement of the overall fragrance quality. Attached Figure Description

[0029] Figure 1 This is an anatomical diagram of the night-blooming cereus.

[0030] Figure 2 This is a statistical chart showing how the fragrance of the night-blooming cereus changes over time during its flowering process. Detailed Implementation

[0031] The present invention will be further described in detail below with reference to embodiments, but the implementation of the present invention is not limited thereto.

[0032] Example 1 To facilitate public understanding of the present invention, this embodiment provides a perfume preparation scheme using epiphyllum extract as the core fragrance ingredient. It fully utilizes the unique natural fragrance of epiphyllum, combined with scientific extraction technology and precise formula ratio, to prepare a high-end perfume with the characteristics of epiphyllum's "fresh and clear fragrance at the beginning of bloom - rich and sweet fragrance in the middle - elegant aftertaste" and stable quality. At the same time, it realizes the efficient utilization of epiphyllum resources and enhances its economic value.

[0033] 1. Collection and pretreatment of Epiphyllum oxypetalum raw materials.

[0034] 1.1. Collection site.

[0035] refer to Figure 1The raw material is the perianth segments of the epiphyllum during its peak blooming period. This part, which includes petals and sepals, is the concentrated part of the epiphyllum's main active ingredients (such as polysaccharides and flavonoids) and aromatic substances, providing a rich and pure epiphyllum fragrance base for perfume.

[0036] 1.2. Data collection time.

[0037] Research revealed that the release of the fragrance from the night-blooming cereus exhibits significant time-sensitivity and dynamic changes. The aroma is primarily released during the flower's opening period, and the release pattern of the fragrance components shows a highly dynamic nature. Specifically, from around 8 PM when the flower begins to open until midnight when it is fully open, a period of six hours, the release of volatile compounds from the night-blooming cereus fragrance increases dramatically by 200 times. This increase primarily begins around 10 PM, peaks at 1 AM, and gradually decreases after 2 AM. Figure 2 In addition, among the fragrance components of Epiphyllum oxypetalum, the core component, geraniol, which accounts for 72.54% of the total release, exhibits a release pattern that is completely consistent with the overall volatile matter release pattern. However, the release of the other two main components shows unique temporal characteristics. Methyl salicylate has two release peak periods, namely 10-11 pm and 6-7 am the next day, while benzyl alcohol's release peak is concentrated between 2-4 am.

[0038] Based on the release pattern of aromatic substances from the night-blooming cereus, collection was conducted between 10 PM and 2 AM. During this period, the flowers are full-bodied, brightly colored, and have a high content of effective components. Among them, geraniol, the main aromatic component accounting for approximately 72.54% of the total release, reaches its peak release, maximizing the extraction of the core aromatic substances from the flower. Simultaneously, rainy days and nights with high air humidity were avoided to prevent excessive water content in the flowers from affecting subsequent processing.

[0039] 1.3. Data collection method.

[0040] Using sterilized, sharp scissors, harvesters hold the base of the night-blooming cereus flower with one hand and use the scissors to cut the stem about 1-2 cm from the plant, ensuring the stem remains intact and the flower itself is not damaged. The cut flowers are then placed in a clean, soft, shallow basket in a single layer to avoid squeezing and deforming or damaging them, thus reducing the risk of microbial infection. 2. Save the data after collection.

[0041] After harvesting, the epiphyllum flowers are immediately transferred to the processing workshop. Flowers with pests or diseases are selected out, and the remaining high-quality flowers are quickly placed in liquid nitrogen for preservation (dry ice can also be used if the company's production conditions are limited) in order to lock in the original components of the epiphyllum flower fragrance to the maximum extent and ensure the excellent quality of the raw materials extracted later.

[0042] 3. Preparation of Epiphyllum extract. This embodiment uses four different processes to prepare Epiphyllum extract, namely Epiphyllum extract ① (low-temperature ethanol extraction), Epiphyllum extract ② (supercritical CO2 extraction), Epiphyllum extract ③ (oil extraction), and Epiphyllum extract ④ (steam distillation).

[0043] 3.1 Preparation of Epiphyllum extract ① by low-temperature ethanol extraction, the steps are as follows: a1. Raw material pretreatment: Take out the fresh epiphyllum flowers preserved in liquid nitrogen, remove the odorless parts such as the flower stalk, and keep only the tepals. Quickly rinse the tepals with deionized water, then use sterile gauze to absorb the surface moisture (to avoid diluting the subsequent extraction solvent), and cut them into small pieces of 0.5cm square for later use.

[0044] b1. Use food-grade anhydrous ethanol (purity ≥95%, odorless) as the extraction solvent. Mix the small pieces of tepals with ethanol at a mass-volume ratio of 1:6 and put them into a brown glass bottle with a sealed cap (the brown bottle can avoid light and prevent photo-oxidation from destroying the fragrance substances).

[0045] c1. Low-temperature extraction: Place the brown glass bottle containing the mixture in a refrigerated environment at 4~8℃ and allow it to stand for 24 hours for extraction. The low temperature environment can delay the volatilization of aroma components, and the 24-hour extraction time can ensure that the aroma components are fully dissolved. Furthermore, no stirring is required, avoiding mechanical force that could cause changes in the aroma molecular structure.

[0046] d1. Separation and Purification: After extraction, the mixture was filtered through a 300-mesh sieve to remove petal residue, and the filtrate was collected. The filtrate was transferred to a low-temperature rotary evaporator, with the temperature set at 35℃ (below the boiling point of ethanol 78℃ to avoid high temperature damage to the aroma and loss of aroma components due to bumping), and the vacuum degree at 0.09MPa. Ethanol was slowly evaporated until the filtrate had a viscous essential oil texture. The viscous extract was then diluted with a small amount of deionized water and filtered through a 0.22μm microporous membrane to remove trace impurities, yielding a clear Epiphyllum extract①.

[0047] Storage: Immediately add 0.02% vitamin E (as a natural antioxidant to prevent the aroma components from oxidizing and deteriorating) to the epiphyllum extract①, seal and store at -18℃ for later use. The extract of Epiphyllum oxypetalum ① was analyzed by gas chromatography-mass spectrometry and liquid chromatography. The obtained extract ① contained volatile aroma components and non-volatile components. The volatile aroma components included geraniol, benzyl alcohol, linalool, benzaldehyde and its glycosides; the non-volatile components included flavonoids (such as kaempferol and quercetin glycosides), phenolic acids, polysaccharides, amino acids, pigments, and trace waxes. The extract ① contained 62.5% geraniol, 9.9% benzyl alcohol, and 1.1% linalool.

[0048] 3.2. The following steps were taken to prepare Epiphyllum extract ② using supercritical CO2 extraction: a2. Raw material pretreatment: Fresh epiphyllum flowers preserved in liquid nitrogen are freeze-dried at -50℃ under vacuum to avoid damaging the aroma compounds. The dried epiphyllum flowers are then pulverized into 80-mesh powder to increase the contact area with the extractant and improve extraction efficiency.

[0049] b2. Supercritical extraction operation: Put the epiphyllum powder into the supercritical extraction equipment and set the extraction parameters: extraction pressure 30MPa (at this pressure, the solubility of aroma components by supercritical CO2 is optimal), extraction temperature 35℃ (low temperature can protect the aroma components from being destroyed), CO2 flow rate 20L / h, extraction time 2h, to ensure that all volatile aroma components are fully dissolved.

[0050] c2. Separation and Collection: The extract was processed using a two-stage separation method. The first-stage separation was set at a pressure of 10 MPa and a temperature of 40°C, mainly for removing impurities; the second-stage separation was set at a pressure of 5 MPa and a temperature of 30°C, and the pale yellow, transparent, and fragrant pure Epiphyllum extract was collected.

[0051] Storage: Store the Epiphyllum extract ② in a dark, sealed glass bottle in a cool, dry place at a temperature <20℃ for later use.

[0052] The extract of Epiphyllum oxypetalum ② was analyzed by gas chromatography-mass spectrometry and liquid chromatography. The main components of the extract were volatile aroma compounds and lipid-soluble substances. These included terpenes such as ocimene, farnesene, and caryophyllene; esters such as benzyl acetate, methyl salicylate, benzyl salicylate, and benzyl benzoate; alcohols such as geraniol, linalool, benzyl alcohol, and nerol; and aldehydes and ketones such as citral, nonanal, and benzaldehyde. The extract also showed that geraniol accounted for 75.1%, methyl salicylate 4.4%, citral 2.3%, and benzyl alcohol 10.3%.

[0053] 3.3 Preparation of Epiphyllum extract ③ using oil extraction method, the steps are as follows: a3. Raw material preparation: Take out the fresh epiphyllum flowers preserved in liquid nitrogen, remove the flower stems, wash and drain the tepals, and tear them into thin strips to increase the contact area with the extraction solvent and promote the dissolution of aroma components.

[0054] b3. Use odorless liquid paraffin as the extraction carrier (glycerin can also be used as needed), mix it with the epiphyllum petal filaments and liquid paraffin in a mass ratio of 1:4, and put it into a sealed glass jar.

[0055] c3. Low-temperature extraction: Place the sealed glass jar in a cool, dark place at 10-15℃ (avoid direct sunlight) and let it stand for 7 days for extraction. During the extraction period, gently shake the glass jar once a day to promote the dissolution of aroma components, while avoiding vigorous shaking that could damage the structure of the aroma substances.

[0056] d3. Filtration and separation: After 7 days of extraction, the mixture was filtered through a 200-mesh sieve to remove petal residue and collect the epiphyllum extract ③.

[0057] Storage: Store the Epiphyllum extract ③ in a dark, sealed glass bottle in a cool, dry place at a temperature <20℃ for later use.

[0058] Gas chromatography-mass spectrometry (GC-MS) and liquid chromatography (LC) were used to analyze the extract ③ of Epiphyllum oxypetalum. The main components of extract ③ were found to be highly lipid-soluble compounds, including high-boiling-point terpenes, sesquiterpenes (such as farnesene and bisabolene), long-chain aliphatic hydrocarbons, wax esters, and lipid-soluble aroma precursors. Geraniol was found to be 66.5%, ocimene 6.7%, farnesene 2.4%, and pinene 1.3% in extract ③.

[0059] 3.4. The extract of Epiphyllum oxypetalum was prepared by steam distillation. The specific steps are as follows: a4. Drying and Condensation Collection: Fresh epiphyllum flowers preserved in liquid nitrogen are placed in a forced-air drying oven, and the drying temperature is set to 35℃. A condenser is connected to the exhaust vent of the drying oven. Extracts are collected after the epiphyllum flowers have been dried for about 1 hour. The low-temperature drying environment of 35℃ promotes slow water loss from the cells, preventing cell rupture and loss of components due to high temperature. Water inside the cells seeps through the cell membrane to the surface of the flower, drips into the water collection tray, and enters the condenser.

[0060] b4. Collection frequency and operation: Check the collection container every 30 minutes and use a sterile pipette to transfer the collected extract to a sterile container to prevent contamination or evaporation from prolonged exposure in the oven.

[0061] c4. Filtration and Concentration: The collected extract is immediately subjected to preliminary filtration to remove impurities, followed by fine filtration through a 0.22μm microporous membrane to remove microorganisms. The filtrate is then placed in a vacuum concentration apparatus for low-temperature concentration at below 40℃ to increase the concentration of the active ingredients, yielding the Epiphyllum oxypetalum extract.

[0062] Storage: Place the Epiphyllum extract ④ into a dark-colored sealed glass bottle and store it in a cool, dry place at a temperature <20℃ for later use.

[0063] Gas chromatography-mass spectrometry (GC-MS) and liquid chromatography (LC) were used to analyze the extract ④ of Epiphyllum oxypetalum. The main components of extract ④ were found to be water-soluble and some volatile components. The water-soluble components included sugars (fructose, glucose), pectin, amino acids (aspartic acid, glutamic acid), water-soluble vitamins, glycosides, and "ripe aroma" molecules such as furans and pyrazines produced by thermal effects. The volatile substances included geraniol, geranialdehyde, nerol, benzaldehyde, melonaldehyde, methyl salicylate, citral, benzyl salicylate, benzyl benzoate, and nerol. The measured percentages for Epiphyllum oxypetalum extract ④ were: geraniol 58.5%, methyl salicylate 4.3%, citral 2.4%, benzyl alcohol 10.5%, and benzyl salicylate 1.3%.

[0064] 4. Perfume formulation.

[0065] 4.1 Perfume - The formula for "Moonlight Beauty" (taking the preparation of 1000g of perfume as an example) is shown in Table 1.

[0066] Table 1:

[0067] 4.2 Modulation steps.

[0068] (1) Raw material preparation: Prepare raw materials according to Table 1 above.

[0069] (2) Mixed fragrance: According to the above formula ratio, first dissolve Epiphyllum extract ④ in 2wt% propylene glycol to obtain Epiphyllum extract ④ propylene glycol solution; then add the remaining Epiphyllum extract, neroli ester formate, orange extract, methyl salicylate, isoamyl acetate, geraniol, hexenal, heptanal, Epiphyllum extract, indole, phenethyl alcohol, gardenia extract, vanillin, tuberose main agent, jasmine extract, and peony main agent to a sterile mixing container in sequence, and stir with a sterile stirrer at 50r / min for 15min to ensure that all fragrance components are evenly mixed.

[0070] (3) Dissolving in solvent: Slowly add the well-mixed fragrance into a sterile mixing tank containing dealdehyde alcohol, turn on the stirring function of the mixing tank, and stir at 80r / min for 30min until the fragrance is completely dissolved in the alcohol to form a uniform mixture.

[0071] (4) Static Aging: Seal the prepared mixture and place it in a well-ventilated environment at a temperature of 20-25℃, away from light, for 5-6 weeks. During the aging process, the aroma components are fully integrated and balanced, which can remove some of the irritating odors and improve the roundness and stability of the perfume aroma.

[0072] (5) Filtration: After aging, the mixture is filtered through a 0.22μm microporous membrane to remove trace impurities and insoluble particles, ensuring that the perfume is clear and transparent, without suspended matter and sediment.

[0073] (6) Bottling and storage: The filtered perfume is filled into pre-sterilized dark perfume bottles using aseptic filling equipment (dark bottles can avoid light and prevent the fragrance components from photo-oxidizing and deteriorating), sealed and labeled, and stored in a cool and dry place.

[0074] Perfume quality testing.

[0075] (a) Sensory index detection.

[0076] Scent: A judging panel of 20 professional perfumers evaluated the scent using the olfactory method. The overall sensory score was the sum of scores for appearance, color, and scent. The judging panel's scoring criteria are shown in Table 2 below.

[0077] Table 2

[0078] The test results are shown in Table 3.

[0079] (ii) Physicochemical index testing.

[0080] pH value: The pH value of the perfume should be tested using a precision pH meter. The result should be in the range of 5.5 to 7.0 (close to neutral) to meet the requirements for skin friendliness.

[0081] Soluble solids content: Detected using a refractometer, the soluble solids content of the perfume should be ≤0.5% to ensure low sugar and low impurities, and to avoid affecting the stability and user experience of the perfume. Mercury content: According to Chapter 4, Section 1.2 of the "Cosmetic Safety Technical Specifications" (2015 edition), the limit is ≤1.

[0082] Lead content: According to Chapter 4, Section 1.3 of the "Cosmetic Safety Technical Specifications" (2015 edition), the limit is ≤10.

[0083] Arsenic content: According to Chapter 4, Section 1.4 of the "Cosmetic Safety Technical Specifications" (2015 edition), the limit is ≤2 arsenic content.

[0084] Cadmium content: According to Chapter 4, Section 1.5 of the "Cosmetic Safety Technical Specifications" (2015 edition), the limit is ≤5.

[0085] Methanol content: According to Chapter 4, Section 2.22 of the "Cosmetic Safety Technical Specifications" (2015 edition), gas chromatography method, the limit is ≤2000.

[0086] The test results are shown in Table 3.

[0087] Table 3

[0088] Tests showed that the perfumes prepared using the method described in this embodiment did not deteriorate in fragrance (no "stale" smell was produced after oxidation), had good appearance and color, and had a pH value close to neutral. The microbial indicators all met the requirements of the "Cosmetic Safety Technical Specifications" (2015 edition) and the "Perfume and Cologne" (QB / T 1858-2004) standards, proving that the perfumes have good stability and can meet the needs of market circulation and use.

[0089] Example 2 The only difference between this embodiment and Example 1 is that the proportions of the four components—Epimedium extract ①, Epiphyllum extract ②, Epiphyllum extract ③, and Epiphyllum extract ④—in the raw material are different. The proportions of the raw materials are shown in Table 4.

[0090] Table 4

[0091] The perfume was then prepared using the same method and steps as in Example 1.

[0092] The perfume was then tested for quality using the same testing method as in Example 1, and the results are shown in Table 5.

[0093] Table 5

[0094] Tests showed that the perfumes prepared using the method described in this embodiment did not deteriorate in fragrance (no "stale" smell was produced after oxidation), had good appearance and color, and had a pH value close to neutral. The microbial indicators all met the requirements of the "Cosmetic Safety Technical Specifications" (2015 edition) and the "Perfume and Cologne" (QB / T 1858-2004) standards, proving that the perfumes have good stability and can meet the needs of market circulation and use.

[0095] Example 3 The only difference between this embodiment and Example 1 is that the proportions of the four components—Epimedium extract ①, Epiphyllum extract ②, Epiphyllum extract ③, and Epiphyllum extract ④—in the raw material are different. The proportions of the raw materials are shown in Table 6.

[0096] Table 6

[0097] The perfume was then prepared using the same method and steps as in Example 1.

[0098] The perfume was then tested for quality using the same testing method as in Example 1, and the results are shown in Table 7.

[0099] Table 7

[0100] Tests showed that the perfumes prepared using the method described in this embodiment did not deteriorate in fragrance (no "stale" smell was produced after oxidation), had good appearance and color, and had a pH value close to neutral. The microbial indicators all met the requirements of the "Cosmetic Safety Technical Specifications" (2015 edition) and the "Perfume and Cologne" (QB / T 1858-2004) standards, proving that the perfumes have good stability and can meet the needs of market circulation and use.

[0101] Comparative Example 1 The only difference between this comparative example and Example 1 is that the epiphyllum extract used in this example is epiphyllum extract①, with a dosage of 140g. The other raw materials and their dosages are the same as in the example.

[0102] The steps and methods for preparing the perfume are the same as in Example 1, and the perfume is obtained.

[0103] Similarly, the perfume of this comparative example was tested using the same quality testing method as in Example 1, and the test results are shown in Table 8.

[0104] Comparative Example 2 The only difference between this comparative example and Example 1 is that the epiphyllum extract used in this example is epiphyllum extract ②, with a dosage of 140g. The other raw materials and their dosages are the same as in the example.

[0105] The steps and methods for preparing the perfume are the same as in Example 1, and the perfume is obtained.

[0106] Similarly, the perfume of this comparative example was tested using the same quality testing method as in Example 1, and the test results are shown in Table 8.

[0107] Comparative Example 3 The only difference between this comparative example and Example 1 is that the epiphyllum extract used in this example is epiphyllum extract ③, with a dosage of 140g. The other raw materials and their dosages are the same as in the example.

[0108] The steps and methods for preparing the perfume are the same as in Example 1, and the perfume is obtained.

[0109] Similarly, the perfume of this comparative example was tested using the same quality testing method as in Example 1, and the test results are shown in Table 8.

[0110] Comparative Example 4 The only difference between this comparative example and Example 1 is that the epiphyllum extract used in this example is epiphyllum extract ④, with a dosage of 140g. The other raw materials and their dosages are the same as in the example.

[0111] The steps and methods for preparing the perfume are the same as in Example 1, and the perfume is obtained.

[0112] Similarly, the perfume of this comparative example was tested using the same quality testing method as in Example 1, and the test results are shown in Table 8.

[0113] Comparative Example 5 The only difference between this comparative example and Example 1 is that the raw materials used are 70g of Epiphyllum extract① + 70g of Epiphyllum extract②, while the other raw materials and their amounts are the same as in the example.

[0114] The steps and methods for preparing the perfume are the same as in Example 1, and the perfume is obtained.

[0115] Similarly, the perfume of this comparative example was tested using the same quality testing method as in Example 1, and the test results are shown in Table 8.

[0116] Comparative Example 6 The only difference between this comparative example and Example 1 is that the raw materials used are 70g of Epiphyllum extract ② + 70g of Epiphyllum extract ④, while the other raw materials and their amounts are the same as in the example.

[0117] The steps and methods for preparing the perfume are the same as in Example 1, and the perfume is obtained.

[0118] Similarly, the perfume of this comparative example was tested using the same quality testing method as in Example 1, and the test results are shown in Table 8.

[0119] Table 8

[0120] A comparison of the perfumes prepared in Example 1 and Comparative Examples 1-4 shows that: The perfume, prepared using only Epiphyllum extract① as the main ingredient, has a rich and complex aroma with a slightly "dull" or "solvent-based" feel. It is layered with multiple notes, including floral, fruity, and green notes, but the most premium and lightest aroma may be somewhat lost, and the fragrance has moderate sillage.

[0121] This perfume, prepared using only Epiphyllum extract② as its main ingredient, has a fragrance that most closely resembles the natural Epiphyllum flower, exhibiting high purity, fullness, freshness, and rich layers. From the light and airy top notes to the mellow body notes, everything is exceptionally well preserved. It boasts high fragrance intensity, strong diffusion, and no solvent odor, making it of the highest quality. However, if its concentration is too high, it will completely mask the delicate aromas of other extracts, resulting in a "flat" fragrance with only a pungent top note and a lack of middle and base notes.

[0122] This perfume, made primarily from Epiphyllum extract, has a soft, sweet, and long-lasting scent, but lacks freshness and complexity. The fragrance leans more towards a warm, powdery base note (similar to the scent of directly dried Epiphyllum) rather than the explosive aroma of fresh flowers. It has weak sillage but long-lasting fragrance.

[0123] Perfumes made using only epiphyllum extract④ as the main ingredient have a weak fragrance, with a light scent reminiscent of "steaming," "cooking," or "vegetable juice," rather than a strong floral aroma. Their fragrance emphasizes the "taste" imparted by water-soluble components.

[0124] As can be seen from Comparative Examples 5 and 6, the perfumes obtained by using Epiphyllum extract ② with Epiphyllum extract ① and Epiphyllum extract ④ respectively are better than the perfumes prepared by using Epiphyllum extract ② alone, as they neutralize the pungent top notes. However, compared with Examples 1 to 3, they still lack the freshness of the fragrance, the long-lasting effect of the fragrance, and the richness of the layers.

[0125] In Examples 1-3, the Epiphyllum perfumes prepared by blending multiple Epiphyllum extracts emphasize freshness, long-lasting fragrance, and rich layers of aroma, ultimately constructing a harmonious, stable, and layered core fragrance that evolves elegantly over time. It should be noted that each Epiphyllum extract is not a single compound, but a complex mixture system composed of multiple components with different chemical properties. Based on this characteristic, by selecting and combining different extracts, the advantages of each extract can be fully utilized to achieve synergistic effects and further optimize the overall fragrance performance. Furthermore, the blending ratios of Epiphyllum extracts ①, ②, ③, and ④ can be flexibly adjusted according to actual application scenarios or market demands to meet diverse fragrance customization needs.

[0126] The inventors of this invention discovered through extensive experimentation that: First, the aroma intensity of the four extracts varies considerably.

[0127] The four extracts of Epiphyllum oxypetalum—①, ②, ③, and ④—exhibit significant differences in aroma intensity. Extract ② typically has the strongest aroma due to its high concentration of volatile aromatic molecules. However, if the proportion is too high, it can completely mask the delicate aromas of the other extracts, resulting in a "flat" fragrance with only a pungent top note and a lack of middle and base notes. Extract ④ has the weakest aroma; if the proportion is too low, its "watery" and "fresh" qualities will completely disappear; if the proportion is too high, it may introduce an unpleasant "steamed" smell.

[0128] Second, the four extracts have different volatility and aroma levels.

[0129] Epiphyllum extract ② is rich in top notes (such as monoterpenes and low molecular weight aldehydes), which evaporate the fastest and are smelled immediately; Epiphyllum extract ① contains more body notes / middle notes (such as alcohols and esters) and base notes / base notes (such as sesquiterpenes and waxes), which evaporate slowly and are responsible for the core characteristics and long-lasting fragrance of the perfume; Epiphyllum extract ④ mainly provides a "watery" feel and a slight top note support.

[0130] III. Solubility and compatibility issues of the four extracts.

[0131] This is also the most critical technical bottleneck. The base solvent of perfume is ethanol, which has excellent compatibility with Epiphyllum extract①, Epiphyllum extract②, and Epiphyllum extract③. However, Epiphyllum extract④ is water-soluble, and directly adding it to ethanol-based perfumes will cause turbidity, flocculation, or even layering, ruining the perfume's clear appearance and stability. It is necessary to premix it with a co-solvent (such as a "water-ethanol" co-solvent system) to ensure that it is completely dissolved in the final product.

[0132] IV. Sensory coordination and concealment.

[0133] Epiphyllum extract ③ has a soft, sweet fragrance that can soften the overly sharp aroma of Epiphyllum extract ②. The broad-spectrum components in Epiphyllum extract ① provide a rich background, increasing the thickness and complexity of the aroma. The "steamed" note of Epiphyllum extract ④ needs to be masked and modified by the strong, fresh floral scent of Epiphyllum extract ②. By "tailoring" the scent through fragrance proportions, the synergistic effect between components is utilized to create a better effect, and the advantages are used to cover up the disadvantages.

[0134] In this embodiment, through scientific collection and pretreatment processes of Epiphyllum oxypetalum raw materials, combined with four different methods for preparing Epiphyllum oxypetalum extracts, the natural aroma components of Epiphyllum oxypetalum were fully preserved. Furthermore, through precise perfume formulation and standardized blending procedures, a high-quality Epiphyllum oxypetalum perfume was successfully prepared. This perfume not only possesses the unique aroma layers of Epiphyllum oxypetalum but also incorporates the elegant aromas of various auxiliary fragrances, and all quality indicators meet the requirements, demonstrating good stability. Simultaneously, this embodiment utilizes the flavor substances that might otherwise be lost during the drying process of Epiphyllum oxypetalum (Epiphyllum oxypetalum extract④ prepared by condensation collection) in perfume blending, achieving efficient utilization of Epiphyllum oxypetalum resources, enhancing its economic value, aligning with the concept of green and sustainable development, and possessing good prospects for industrial application and market competitiveness.

[0135] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention in any way. Any simple modifications or equivalent changes made to the above embodiments based on the technical essence of the present invention shall fall within the protection scope of the present invention.

Claims

1. The application of an extract of Epiphyllum oxypetalum in the preparation of perfume, characterized in that: The epiphyllum extract is composed of epiphyllum extract ①, epiphyllum extract ②, epiphyllum extract ③, and epiphyllum extract ④. The Epiphyllum extract ① is an extract obtained by low-temperature ethanol extraction, and contains geraniol ≥ 62.5%, benzyl alcohol ≥ 9.8%, and linalool ≥ 1.06% in Epiphyllum extract ①. The Epiphyllum extract ② is an extract obtained by supercritical CO2 extraction. The Epiphyllum extract ② contains geraniol ≥75%, methyl salicylate ≥4.3%, citral ≥2.23%, and benzyl alcohol ≥10%. The Epiphyllum extract ③ is an extract obtained by oil extraction, and contains geraniol ≥ 66.5%, ocimene ≥ 6.35%, farnesene ≥ 2.3%, and pinene ≥ 1.25%. The Epiphyllum extract ④ is an extract obtained by steam distillation, and contains geraniol ≥ 58%, methyl salicylate ≥ 4.3%, citral ≥ 2.23%, benzyl alcohol ≥ 10%, and benzyl salicylate ≥ 1.22%. The mass fraction ratio of Epiphyllum extract ①: Epiphyllum extract ②: Epiphyllum extract ③: Epiphyllum extract ④ is 10~25:70~85:2~8:0.5~3; The epiphyllum extract is mixed with perfume-acceptable excipients, including at least one of solvents, stabilizers, and fragrance enhancers, and then the mixture is homogeneous to make a perfume.

2. The application according to claim 1, characterized in that: Epiphyllum extract① includes volatile aroma components and non-volatile components. The volatile aroma components include geraniol, benzyl alcohol, linalool, benzaldehyde and its glycosides; the non-volatile components include flavonoids, phenolic acids, polysaccharides, amino acids, pigments and waxes.

3. The application according to claim 1, characterized in that: Epiphyllum extract② includes terpenes, esters, alcohols, aldehydes and ketones, volatile aroma compounds, and fat-soluble substances; Among them: terpenes include ocimene, farnesene, and caryophyllene; esters include benzyl acetate, methyl salicylate, benzyl salicylate, and benzyl benzoate; alcohols include geraniol, linalool, benzyl alcohol, and nerol; and aldehydes and ketones include citral, nonanal, and benzaldehyde.

4. The application according to claim 1, characterized in that: Epiphyllum extract③ contains highly lipid-soluble compounds, including sesquiterpenes, long-chain aliphatic hydrocarbons, wax esters, and lipid-soluble aroma precursors.

5. The application according to claim 1, characterized in that: Epiphyllum extract ④ includes water-soluble components and some volatile substances. Water-soluble substances include sugars, pectin, amino acids, water-soluble vitamins, and glycosides; volatile substances include geraniol, geranialdehyde, nerol, benzaldehyde, melon aldehyde, methyl salicylate, citral, benzyl salicylate, benzyl benzoate, and nerol.

6. The application according to claim 1, characterized in that, The steps for obtaining Epiphyllum extract① by ethanol extraction are as follows: a1. Raw material harvesting: Select epiphyllum flowers in full bloom and harvest the petals between 10 pm and 2 am. Use a sterilized sharp tool to cut the petals 1-2 cm away from the plant. After harvesting, place them in a single layer in a clean and soft container, avoiding rainy days or nights with high humidity. b1. Immediately after harvesting, transfer the flowers to the processing workshop, remove diseased and damaged perianth pieces, and then preserve them with liquid nitrogen or dry ice. After quickly rinsing the perianth pieces with deionized water, absorb the moisture and cut them into small square pieces of 0.5±0.8cm. c1. Mix Epiphyllum oxypetalum and food-grade anhydrous ethanol at a mass-to-volume ratio of 1:6 to 1:9, put them into a brown glass bottle with a sealed cap, and extract at 4 to 8°C for 24 to 28 hours. d1. After extraction, filter the residue through a 300-mesh sieve. The filtrate is then rotary evaporated at 35~40℃ and -0.09~-0.1MPa until it becomes viscous. After dilution, filter the filtrate through a 0.22~0.28μm microporous membrane and collect the filtrate, which is the Epiphyllum extract①.

7. The application according to claim 1, characterized in that, The steps for obtaining Epiphyllum extract ② by ethanol extraction are as follows: a2. Raw material harvesting: Select epiphyllum flowers in full bloom and harvest the petals between 10 pm and 2 am. Use a sterilized sharp tool to cut the petals 1-2 cm away from the plant. After harvesting, place them in a single layer in a clean and soft container, avoiding rainy days or nights with high humidity. b2. Immediately after harvesting, transfer the flowers to the processing workshop, remove diseased and damaged perianth pieces, and then preserve them with liquid nitrogen or dry ice. After vacuum freeze-drying the perianth pieces at -50℃, pulverize them into 80-mesh powder. c2. The Epiphyllum powder in step b2 is extracted using supercritical CO2. The extraction parameters are set as follows: extraction pressure 30~35MPa, extraction temperature 35~39℃, CO2 flow rate 20~24L / h, and extraction time 2~2.5h. d2. Then, a two-stage separation method is used to remove impurities. The parameters for the first-stage separation are pressure 10~13MPa and temperature 40~43℃, and the parameters for the second-stage separation are pressure 5MPa and temperature 30~34℃. The separated extract is collected, which is the epiphyllum extract ②.

8. The application according to claim 1, characterized in that, The steps for obtaining Epiphyllum extract ③ by ethanol extraction are as follows: a3. Raw material harvesting: Select epiphyllum flowers in full bloom and harvest the tepals from 10 pm to 2 am. Use a sterilized sharp tool to cut the flower stalk 1-2 cm away from the plant. After harvesting, place them in a single layer in a clean and soft container, avoiding rainy days or nights with high humidity. b3. Immediately after harvesting, transfer the flowers to the processing workshop, remove diseased and damaged perianth pieces, and then preserve them with liquid nitrogen or dry ice. Wash and drain the perianth pieces and tear them into thin strips. c3. Use odorless liquid paraffin or glycerin as a carrier, mix petals and carrier at a mass ratio of 1:4~5, put into a sealed glass jar, and let it stand for 7 days in a cool, dark place at 10~15℃ for extraction. d3. After extraction, filter through a 200-mesh sieve to remove petal residue and collect the extract, which is the epiphyllum extract ③.

9. The application according to claim 1, characterized in that, The steps for obtaining Epiphyllum extract ④ by ethanol extraction are as follows: a4. Raw material harvesting: Select epiphyllum flowers in full bloom and harvest the tepals from 10 pm to 2 am. Use a sterilized sharp tool to cut the flower stalk 1-2 cm away from the plant. After harvesting, place them in a single layer in a clean and soft container, avoiding rainy days or nights with high humidity. b4. Immediately after harvesting, transfer the flowers to the processing workshop, remove diseased and damaged perianth pieces, and then preserve them with liquid nitrogen or dry ice. Place the perianth pieces in a drying oven. c4. Dry the perianth pieces at 35~42℃ with forced air. Connect a condenser to the exhaust vent of the drying oven. After drying for 1~2 hours, start collecting the volatile flavor substances. Every 30~35 minutes, use a sterile pipette to transfer the collected extracts to a sterile container. d4. After collecting the extract, filter it immediately to remove impurities, then filter it through a 0.22~0.28μm microporous membrane, and then concentrate it under reduced pressure at 40~45℃ to obtain the concentrated solution, which is the epiphyllum extract ④.

10. The application according to claim 1, characterized in that: The sensory indicators of the Epiphyllum extract are as follows: clear and transparent in appearance, free of suspended matter and sediment, colorless or slightly yellow in color, and with the unique fragrance of Epiphyllum without any off-odor.

11. The application according to claim 1, characterized in that: The epiphyllum extract is prepared according to any one of the following: a) Add 0.02~0.03wt% vitamin E or rosemary extract, seal, and freeze at -18℃; b) Store in a dark, sealed glass bottle at a temperature below 20°C in a cool, dry place.

12. The application according to claim 1, characterized in that, The preparation of perfume includes the following steps: (1) Raw material preparation: Prepare raw materials according to the formula. The raw materials include Epiphyllum extract and excipients. Epiphyllum extract accounts for 11.5~15.0% of the total mass of raw materials. The excipients include dialdehyde alcohol, nerol formate, orange extract, methyl salicylate, isoamyl acetate, geraniol, hexenal, heptanal, Epiphyllum extract, indole, phenylethanol, gardenia extract, vanillin, tuberose main agent, jasmine extract, peony main agent, and propylene glycol. (2) Mixed fragrance: First, dissolve Epiphyllum extract ④ in propylene glycol, with a concentration of 1.5~2wt%, to obtain a propylene glycol solution of Epiphyllum extract ④; then mix the remaining Epiphyllum extracts with the excipients in a set ratio and stir evenly. (3) Dissolving in solvent: Add the mixed fragrance to the dialdehyde alcohol and stir until completely dissolved; (4) Let stand and age: Let the dissolved mixture stand for 5 to 6 weeks; (5) Filtration: The aged mixture is finely filtered through a microporous membrane to remove impurities; (6) Bottle storage: Put the filtered perfume into a perfume bottle and seal it for storage.

13. The application according to claim 7, characterized in that: In step (2), when mixing, first mix the epiphyllum extract, epiphyllum extract, orange blossom extract, gardenia extract, and jasmine extract evenly, then add neroli ester formate, methyl salicylate, isoamyl acetate, geraniol, hexenal, heptanal, indole, phenylethanol, vanillin, tuberose main agent, and peony main agent and continue stirring.

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