Five-day biochemical oxygen demand monitoring equipment for centralized full-automatic analysis in laboratory

Abstract

The invention discloses laboratory centralized full-automatic analysis five-day biochemical oxygen demand monitoring equipment, which relates to the technical field of five-day biochemical oxygen demand monitoring equipment, and comprises a laboratory centralized full-automatic analysis system, a sample temporary storage incubator, an incubator, a sample transport vehicle and a pretreatment system, the pretreatment system is arranged at the side part of the laboratory centralized full-automatic analysis system. According to the laboratory centralized full-automatic analysis five-day biochemical oxygen demand monitoring equipment, the sample temporary storage constant temperature box, the culture box, the sample transport vehicle, the pretreatment system and other structures are arranged, so that the defect of a laboratory centralized full-automatic analysis system in the aspect of five-day biochemical oxygen demand monitoring is overcome, and the overall automation level of a laboratory is improved; tedious consumable preparation of a laboratory is simplified, operation of experimenters can be greatly simplified, personal errors in the experiment process are reduced, and result accuracy is guaranteed.

Description

Technical Field

[0001] This invention relates to the technical field of five-day biochemical oxygen demand (BOD) monitoring equipment, and particularly to a five-day biochemical oxygen demand (BOD) monitoring equipment for centralized, fully automated laboratory analysis. Background Technology

[0002] The centralized, fully automated laboratory analysis system is an emerging monitoring method characterized by high automation, high sample throughput, and highly controllable analysis processes. It can perform centralized, fully automated analysis of all laboratory analytical items listed in Table 1 of the "Surface Water Quality Standard," excluding five-day biochemical oxygen demand (BOD5). Unlike traditional automated monitoring equipment that targets a single item, the centralized, fully automated laboratory analysis system can analyze multiple items simultaneously, significantly improving work efficiency. Currently, some monitoring institutions have begun practical applications of this system.

[0003] Spectrophotometry and volumetric methods, due to their numerous monitoring methods and early automation implementation, can now be integrated into centralized, fully automated laboratory analysis systems. However, 5-day biochemical oxygen demand (BOD5), due to its complex analytical process and difficulty in controlling experimental conditions, is still far from being integrated into such systems. BOD5 is a monitoring item in Table 1 of the "Surface Water Environmental Quality Standard," with high monitoring frequency and a large number of samples. Given limited personnel, conducting monitoring independently of the centralized, fully automated laboratory analysis system would significantly reduce its efficiency. Maintaining two separate teams—one for centralized automated analysis and one for manual monitoring—also places a considerable burden on testing institutions.

[0004] Therefore, it is necessary to propose a five-day biochemical oxygen demand (BOD) monitoring device with centralized, fully automated laboratory analysis to solve the above problems. Summary of the Invention

[0005] The purpose of this invention is to provide a five-day biochemical oxygen demand (BOD) monitoring device for centralized, fully automated laboratory analysis. This addresses the problem that, under limited personnel conditions, conducting monitoring independently of the centralized, fully automated laboratory analysis system would significantly reduce the efficiency of the system. Furthermore, maintaining two separate teams for both centralized, fully automated laboratory analysis and manual monitoring would place a considerable burden on the testing organization.

[0006] To achieve the above objectives, the present invention provides the following technical solution: a laboratory-centralized fully automated five-day biochemical oxygen demand monitoring device, comprising a laboratory-centralized fully automated analysis system, a sample storage incubator, an incubator, a sample transport vehicle, and a pretreatment system;

[0007] The pretreatment system is located on the side of the centralized fully automated analysis system in the laboratory and is connected to it via a conveyor belt.

[0008] The sample storage incubator and incubator are used for the constant temperature storage and incubation of samples, respectively, and the sample transport vehicle is used to transport samples between the sample storage incubator, incubator and pretreatment system.

[0009] Preferably, the pretreatment system includes a pretreatment module and a filling module. The pretreatment module is divided into a lower part and an upper part, with the upper part positioned above the lower part.

[0010] The lower part of the module contains a reagent box, an air pump, a pure water tank, a dilution inoculation water tank, a dilution water tank, an injection pump, a peristaltic pump, and a waste liquid tank, and the components are connected by pipelines.

[0011] The upper part of the module is equipped with a bottle cap placement area, an aeration robotic arm, and an aeration pipeline. The aeration pipeline is connected to an air pump and is used to aerate the sample and dilution water.

[0012] Preferably, the filling module is connected to the pretreatment module and is divided into a filling area and a sample transfer area. The two are connected by a track and a conveyor belt and are separated by a lifting door. The filling area is provided with a blank position and a quality control position for filling blank samples and standard samples respectively. A temperature control device is provided at the top of the filling area.

[0013] Preferably, the syringe pump is connected to the reagent box, pure water box, dilution inoculation water box, dilution water box and sample bottle through pipelines for quantitative addition of reagents, dilution water and sample. The dilution inoculation water box and dilution water box are also connected to the aeration pipeline through pipelines for aeration treatment.

[0014] Preferably, the upper part of the module is provided with a robotic arm, which is connected to a robotic arm. The robotic arm moves along the X and Y axes, and the robotic arm moves up and down along the Z axis. The robotic arm is provided with tubing and a stirring paddle for penetrating into the sample bottle for sampling and stirring. The tubing is connected to an injection pump.

[0015] Preferably, the sample transport vehicle is equipped with a sample placement platform and a robotic arm. The robotic arm is used to grab or push and pull the sample rack to transfer the sample between the sample temporary storage constant temperature box, the incubator and the pretreatment system.

[0016] Preferably, both the pretreatment module and the filling module are equipped with an electrode assembly, which includes a dissolved oxygen electrode and a stirring paddle for reading the dissolved oxygen concentration and stirring the water sample. The electrode assembly is provided with a cleaning position and an air blowing position for cleaning and drying the electrode, respectively. The cleaning position is connected to a peristaltic pump, and the air blowing position is connected to an air pump.

[0017] The technical effects and advantages of this invention are as follows:

[0018] 1. This invention fills the gap in five-day biochemical oxygen demand monitoring in centralized fully automated laboratory analysis systems by setting up a sample storage constant temperature box, incubator, sample transport vehicle and pretreatment system, etc., improves the overall automation level of the laboratory, simplifies the cumbersome preparation of consumables in the laboratory, can greatly simplify the operation of experimental personnel, reduce the occurrence of human error in the experiment, and ensure the accuracy of the results. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of the five-day biochemical oxygen demand monitoring device for centralized, fully automated laboratory analysis according to the present invention.

[0020] Figure 2 This is a schematic diagram of the sample transport vehicle of the present invention.

[0021] Figure 3 This is a schematic diagram of the lower structure of the module of the present invention.

[0022] Figure 4 This is a schematic diagram of the upper structure of the module of the present invention.

[0023] Figure 5 This is a schematic diagram of the filling module structure of the present invention.

[0024] In the diagram: 1. Centralized fully automated laboratory analysis system; 2. Sample storage incubator; 3. Incubator; 4. Sample transport vehicle; 5. Lower part of the module; 501. Reagent box; 502. Air pump; 503. Pure water tank; 504. Dilution inoculation water tank; 505. Dilution water tank; 506. Injection pump; 507. Peristaltic pump; 508. Waste liquid tank; 6. Upper part of the module; 601. Bottle cap placement area; 602. Aeration robotic arm; 603. Aeration pipeline; 7. Filling module; 701. Blank space; 702. Quality control space; 703. Sample transfer area; 704. Lifting door; 8. Electrode assembly. Detailed Implementation

[0025] This invention provides, for example Figures 1-5 The laboratory-based fully automated five-day biochemical oxygen demand (BOD) monitoring equipment shown includes a sample storage incubator 2, an incubator 3, a sample transport vehicle 4, and a pretreatment system located on the side of the laboratory-based fully automated analysis system 1.

[0026] Sample storage incubator 2 is used for temperature control of newly arrived laboratory samples for 2 hours, with adjustable temperature control time; the temperature control range is 0-40℃, and the temperature control accuracy is 20±1℃; it can hold 5 layers of samples, each layer can hold a 20-position sample rack, and can temporarily store a total of 100 sampling bottles, and the capacity can be expanded according to specific usage needs.

[0027] Incubator 3 also has a temperature control accuracy of 20±1℃. It can hold 5 layers of samples, each layer can accommodate 2 12-position sample racks, and can hold a total of 120 samples at the same time. The capacity can be expanded according to specific usage needs.

[0028] Sample transport vehicle 4 is used for sample transport and is equipped with a sample placement platform. The sample placement platform can be raised and lowered to meet different height requirements. When transporting samples, the sample rack is placed on the sample placement platform.

[0029] The sample transport vehicle 4 is equipped with a robotic arm that can automatically grab or push and pull the sample racks in the sample temporary storage constant temperature box 2 and the incubator 3 and place them on the sample placement platform.

[0030] The laboratory centralized fully automated analysis system 1 is equipped with an automatic sample dispensing workstation 101 for dispensing samples. It is equipped with a robotic arm that can identify sample information and place the sampling bottle on the conveyor belt of the laboratory centralized fully automated analysis system 1.

[0031] The pretreatment system includes a pretreatment module and a filling module 7. The pretreatment system is mounted on the laboratory centralized fully automated analysis system 1 and is connected to the laboratory centralized fully automated analysis system 1 via a conveyor belt. It can receive or return samples distributed by the automatic sample divider via the conveyor belt.

[0032] The preprocessing module is divided into a lower module 5 and an upper module 6, with the upper module 6 located above the lower module 5.

[0033] The lower part 5 of the module contains a reagent box 501, an air pump 502, a pure water tank 503, a dilution inoculation water tank 504, a dilution water tank 505, an injection pump 506, a peristaltic pump 507, a waste liquid tank 508, and an air conditioner.

[0034] The reagent box 501 can hold at least 7 bottles of reagents, including 1 bottle of inoculum, 4 bottles of nutrient salt solution, phosphate buffer solution, magnesium sulfate solution, calcium chloride solution, ferric chloride solution, 1 bottle of nitrification inhibitor solution, and 1 bottle of laboratory standard sample solution. The reagent bottles are no less than 1000ml in size.

[0035] Multiple syringe pumps 506 are provided, and the reagents in the reagent box 501 are connected to the corresponding syringe pumps 506 through tubing.

[0036] Pure water tank 503, dilution inoculation water tank 504, dilution water tank 505, and waste liquid tank 508 all have a volume of 80L and are equipped with level gauges, which can be used to monitor the liquid level on the centralized fully automated analysis system 1 in the laboratory. The system will issue an alert when the liquid level reaches 10% and 90%. Pure water tank 503, dilution inoculation water tank 504, and dilution water tank 505 are also connected to the centralized fully automated analysis system 1 in the laboratory via pipelines. The centralized fully automated analysis system 1 in the laboratory supplies pure water to pure water tank 503, dilution inoculation water tank 504, and dilution water tank 505, and the supply volume is adjustable.

[0037] The dilution inoculation tank 504 and the dilution tank 505 are also connected to an aeration pipe 603, which aerates the dilution inoculation tank 504 and the dilution tank 505 through an air pump 502, and the aeration time is adjustable.

[0038] The dilution tank 504 and the dilution tank 505 are equipped with pipelines connected to the injection pump 506.

[0039] Waste liquid tank 508 receives waste liquid discharged from cleaning, drying, and reagent draining processes.

[0040] The syringe pump 506 has two sets of liquid inlets and two sets of liquid outlets in its connecting pipeline.

[0041] The syringe pump 506, used for adding reagents, is connected at one end to the reagents in the reagent tank 501 and at the other end to the dilution inoculation tank 504 and the dilution tank 505. It can supply four kinds of nutrient salt solutions to the dilution inoculation tank 504 and the dilution tank 505 at a ratio of 1 ml / L. It can supply the inoculation solution to the dilution inoculation tank 504 according to the volume required by the inoculation solution instructions. It can add nitrification inhibitors according to the preset program based on the sample characteristics, and the amount of the above reagents added is adjustable.

[0042] The syringe pump 506, used for adding samples, is connected at one end to the laboratory standard sample solution reagent bottle, dilution water tank 505, dilution inoculation water tank 504 and sample bottle in the reagent box 501, and at the other end to the culture bottle. It can inject the sample and various solutions connected to the front end of the syringe pump 506 into the culture bottle according to the preset ratio.

[0043] Furthermore, five temperature sensors are installed at the bottom of the module to work with the air conditioner, which can control the temperature inside the equipment at 20±1℃. The temperature sensors and their working principle are common technologies and will not be described in detail here.

[0044] The upper part 6 of the module can accommodate 8 samples at the same time and carry out sample pretreatment. The number of samples that can be accommodated can be expanded according to specific usage needs.

[0045] The upper part 6 of the module is equipped with a robotic arm and a bottle cap placement area 601. The robotic arm is connected to the robotic arm, which can move along the X and Y axes, while the robotic arm can move up and down along the Z axis. The robotic arm can remove the sample bottle cap and place it in the bottle cap placement area 601. At the same time, the robotic arm is equipped with a tube that can extend and retract vertically and a stirring paddle, which can penetrate into the sample to draw the sample. The tube is connected to the syringe pump 506, and the sample drawing volume is adjustable.

[0046] A conveyor belt is installed in the upper part 6 of the module. The conveyor belt can operate in both directions to receive or push out samples. When receiving samples, the distance between sample bottles can be controlled at 10cm according to a preset program. The distance is adjustable.

[0047] The upper part 6 of the module is equipped with aeration pipes 603. The aeration pipes 603 are made of silicone and are evenly spaced on the aeration robotic arm 602, and the spacing is adjustable. There are 8 sets of aeration pipes 603, corresponding to the number of samples, and they are connected to the main aeration pipe through a gas distributor. Each set of aeration pipes 603 is equipped with a solenoid valve to control the opening and closing of the air path. The solenoid valve is fixed on the aeration robotic arm 602. The aeration robotic arm 602 can move along the Z-axis, which links the lifting and lowering of the 8 sets of aeration pipes 603. The other end of the aeration pipes 603 is connected to a honeycomb-shaped air outlet with a counterweight to improve aeration efficiency.

[0048] The air pump 502 is connected to the main aeration pipeline and generates gas for aeration of samples, dilution water and dilution inoculum water. The air flow rate is adjustable from 10 to 1000 ml / min and the aeration time is adjustable.

[0049] The filling module 7 is connected to the pretreatment module and is divided into a filling area and a sample transfer area 703. The filling area and the sample transfer area 703 are connected by a track, and a conveyor belt is provided between the tracks to transfer samples.

[0050] The filling area is equipped with a robotic arm that can grasp bottle caps and place them in the bottle cap placement area 601. The robotic arm is connected to a robotic arm and can move along the X, Y, and Z axes. The robotic arm is fixed with a set of tubing outlets connected to the syringe pump 506. The tubing is connected to the pure water tank 503, the dilution inoculation water tank 504, the dilution water tank 505, the reagent tank 501, and the pretreatment module robotic arm. It can inject the sample picked up by the pretreatment module robotic arm into the culture bottle, and can also inject dilution water, dilution inoculation water, laboratory standard samples, inoculation solution, and nitrification inhibitor into the culture bottle according to a preset volume. After the sample filling is completed, pure water can be injected around the bottle cap to form a water seal.

[0051] The filling area has a dedicated blank position 701 for filling dilution water blank samples and dilution inoculation water blank samples; it also has a dedicated quality control position 702 for filling laboratory standard samples. The number of dilution water blank samples, dilution inoculation water blank samples, and laboratory standard samples is set according to the sample quantity. After the blank and laboratory standard samples of this batch of samples are filled, blank position 701 and quality control position 702 can be used for sample filling.

[0052] An air conditioner is also installed at the top of the filling area, which can control the temperature of the filling area at 20±1℃.

[0053] The filling area and sample transfer area 703 are separated by a lifting door 704.

[0054] The filling area can accommodate one 12-position sample rack, and the number of sample positions can be expanded.

[0055] Both the pretreatment module and the filling module 7 are equipped with electrode groups 8, and the electrode groups 8 are equipped with cleaning positions for cleaning the liquid addition pipeline.

[0056] The electrode group 8 at the filling module 7 includes one dissolved oxygen electrode and one stirring paddle, used to read chemical oxygen demand and dissolved oxygen concentration, and to stir and mix the water sample; the electrode group 8 at the pretreatment module includes one chemical oxygen demand coarse measurement electrode, one dissolved oxygen electrode and one stirring paddle.

[0057] In addition, another robotic arm in the filling area is also connected to electrode group 8, which includes a dissolved oxygen electrode and a stirring paddle, and can mix the filled sample and read the solution oxygen concentration of the sample.

[0058] Electrode assembly 8 is controlled by a robotic arm to move along the X, Y, and Z axes. When dissolved oxygen testing is not underway, electrode assembly 8 is inserted into an electrode protective sleeve containing a small amount of pure water, and electrode assembly 8 does not come into contact with the water in the protective sleeve. A cleaning position is provided on the right side of the electrode protective sleeve, with the outlet connected to a pure water tank 503 for cleaning electrode assembly 8, and the lower end connected to a waste liquid tank 508 to receive the cleaning water. An air blowing position is provided to the right of the cleaning position, with the air outlet connected to an air pump 502 for drying water stains on electrode assembly 8, and the lower end of the air blowing position connected to a waste liquid tank 508 to receive the water blown out during air blowing.

[0059] The specific steps are as follows:

[0060] 1. After the sample arrives at the laboratory and the handover is completed, the sample transport vehicle 4 places the sample on the sample rack and sends it into the sample temporary storage constant temperature chamber 2 to start the sample constant temperature step for 2 hours;

[0061] 2. The laboratory centralized fully automated analysis system 1 supplies pure water to the pure water tank 503, the dilution inoculation water tank 504, and the dilution water tank 505, and the level gauge records the volume of pure water in each tank;

[0062] 3. The sample transport vehicle 4 transports culture bottles from the reagent and consumables warehouse to the sample transfer area 703 of the pretreatment system. The lifting door 704 at the filling module 7 opens, and the sample is transferred into the pretreatment module through the conveyor belt between the tracks.

[0063] 4. The robotic arm starts, removes the culture flask cap, and places it in the cap placement area 601;

[0064] 5. Prepare the four kinds of nutrients, inoculation solution, and laboratory standard sample solution, and place them in reagent box 501 and connect the reagent tubing; start the syringe pump 506 to begin rinsing the tubing of the four kinds of nutrients and inoculation solution; calculate the amount of the four kinds of nutrient solution and inoculation solution to be added based on the volume of pure water in dilution inoculation tank 504 and dilution tank 505, and set the dilution factor based on the known concentration of the laboratory standard sample.

[0065] 6. Add four kinds of nutrient salt solutions to the dilution inoculation tank 504 and the dilution tank 505 respectively through the syringe pump 506, and add the inoculation solution to the dilution inoculation tank 504; start the air pump 502 to aerate the liquids in the pure water tank 503, the dilution inoculation tank 504 and the dilution tank 505 for 1 hour; after aeration is completed, start the syringe pump 506 to rinse the dilution inoculation water, the dilution water and the laboratory standard sample tubing;

[0066] 7. The robotic arm in the filling area moves to the top of the cleaning tank, the robotic arm descends, the pipeline descends, and the injection pump 506 starts to flush the pipeline;

[0067] 8. The robotic arm moves to blank position 701 and descends, the tubing descends and inserts into the culture bottle mouth, and dilution water and dilution inoculum water are injected into the culture bottle on blank position 701 of the filling area through injection pump 506; the tubing is then raised, the robotic arm is raised and moved to the position before the experiment started;

[0068] 9. The robotic arm controls the electrode assembly 8 to be inserted into the culture flask, the agitator is started, and the dissolved oxygen value is read. If the dissolved oxygen concentration is between 8.0 and 9.0, the dissolved oxygen reading is recorded as Do1 for the dilution water blank and the dilution inoculation water blank, and the next step is performed. If it is outside the range, the sample in the culture flask is discarded, and the liquids in the pure water tank 503, the dilution inoculation water tank 504, and the dilution water tank 505 are aerated again.

[0069] 10. The robotic arm moves the electrode to the cleaning position, the peristaltic pump 507 starts, and the electrode assembly 8 is rinsed with pure water; the robotic arm moves the electrode to the air blowing position, the air pump 502 starts, and the moisture on the electrode assembly 8 is dried through the gas pipeline, in preparation for the analysis of the next sample.

[0070] 11. Start the syringe pump 506 and inject the diluted inoculum water and laboratory standard sample into the quality control station 702 according to the dilution factor of the laboratory standard sample; start the robot arm in the filling area to control the dissolved oxygen electrode to insert into the culture bottle, read the dissolved oxygen value and record it as the Do1 of the laboratory standard sample; repeat step 10.

[0071] 12. The robotic arm in the filling area picks up the caps of the culture bottles and places them on the samples at blank position 701 and quality control position 702;

[0072] 13. Start the syringe pump 506 and inject pure water into the capped sample as a water seal. Record the time and begin 5 days of incubation.

[0073] 14. After the sample isothermal step is completed, the sample transport vehicle 4 takes out the sample and transports it to the automatic sample sorting workstation 101 of the laboratory centralized fully automated analysis system 1. The sample is scanned with a sample label in the automatic sample sorting workstation 101, and the robotic arm in the automatic sample sorting workstation 101 picks up the sample and moves it to the conveyor belt, which then delivers it to the equipment.

[0074] 15. After the sample arrives at the equipment position, the conveyor belt stops rotating, and the sample pushing device pushes the sample from the conveyor belt to the conveyor belt between the equipment's pretreatment system and the laboratory centralized fully automated analysis system 1; the conveyor belt starts, the sample inlet of the pretreatment system opens, and the conveyor belt delivers the sample to the pretreatment system;

[0075] 16. The robotic arm removes the sample bottle caps and places them in the bottle cap placement area 601 in sequence;

[0076] 17. Based on the information obtained by scanning the sample label in the automatic sample sorting workstation, identify the number and location of samples in the pretreatment system; the aeration robotic arm 602 is raised along the track, moved above the sample bottle, and lowered along the track, so that the aeration pipe 603 extends to 1 / 2 of the depth of the sample bottle, and the solenoid valve is opened.

[0077] 18. Turn on air pump 502 to begin aeration of the sample for 30 minutes; after aeration is complete, move the aeration robotic arm 602 to the position before the experiment started and close the solenoid valve.

[0078] 19. Power on the dissolved oxygen electrode and begin calibration using water-saturated air within the electrode protective sleeve;

[0079] 20. The robotic arm moves above the first sample, and the control electrode assembly 8 is lowered and inserted into the sample to read the chemical oxygen demand (COD) concentration. When the COD concentration is ≤20 mg / L, the sample is not diluted. When the COD concentration is between 20 and 30 mg / L, the sample is diluted by 2 times, and so on. For every 10 mg / L increase in COD concentration, the dilution factor is doubled. The stirring paddle is started, and the dissolved oxygen electrode begins to read the dissolved oxygen concentration. After reading is completed, step 10 is repeated, and the test of the next sample begins.

[0080] 21. Repeat step 20 until all samples have been aerated. If the dissolved oxygen concentration of the sample is between 8.0 and 9.0, proceed to the next step. If the dissolved oxygen concentration is not between 8.0 and 9.0, repeat steps 17-18 for the samples with dissolved oxygen concentrations not between 8.0 and 9.0.

[0081] 22. Electrode assembly 8 is inserted into the electrode protective sleeve; the robotic arm moves above the sample, and after the robotic arm descends, the tubing is lowered and inserted into the sample;

[0082] 23. The robotic arm in the filling area moves to the top of the cleaning tank, the robotic arm descends, the pipeline descends, and the injection pump 506 starts to flush the pipeline;

[0083] 24. After rinsing, the robotic arm in the filling area moves above the culture bottle and lowers, with the tubing descending to the bottle opening. Based on the dilution ratio, the sample and dilution water are quantitatively injected into the culture bottle. After completion, the robotic arm moves above the cleaning tank.

[0084] 25. The robotic arm controls the insertion of electrode assembly 8 into the culture flask, starts the stirrer, reads and records the oxygen concentration of the solution as the Do1 of the sample;

[0085] 26. Repeat step 10 in the filling area;

[0086] 27. In the filling area, the robotic arm picks up the caps of the culture bottles and places them on the culture bottles;

[0087] 28. Start the syringe pump 506 and inject pure water into the capped sample as a water seal. Record the time and begin 5 days of incubation.

[0088] 29. Repeat steps 20-28 to analyze the remaining sample;

[0089] 30. After all samples have been analyzed, the lifting door 704 at the entrance and exit of the filling area is opened, and the conveyor belt between the tracks is started to move the samples from the filling area to the sample transfer area 703.

[0090] 31. The sample transport vehicle 4 moves the sample to the sample placement platform using its own robotic arm, and then moves the sample transport vehicle 4 to the front of the incubator 3;

[0091] 32. The incubator 3 opens automatically, the sample transport cart 4 pushes the sample into the incubator 3, the incubator 3 closes automatically, and the incubation begins;

[0092] 33. The conveyor belt in the pretreatment module is started, pushing the sample onto the conveyor belt between the laboratory centralized fully automated analysis system 1 and the pretreatment system. The sample pushing device pulls the sample back onto the conveyor belt, and the conveyor belt sends the sample back to the automatic sample sorting workstation of the laboratory centralized fully automated analysis system 1.

[0093] 34. After 5 days of incubation, the incubator 3 automatically opens its door, and the sample transport vehicle 4 moves the sample to the sample placement platform;

[0094] 35. The sample is transported to the equipment by the sample transport vehicle 4, and the robotic arm moves the sample to the sample transfer area 703;

[0095] 36. The sample entrance / exit lifting door 704 in the filling area opens, and the conveyor belt between the tracks starts to transport the sample to the filling area;

[0096] 37. The robotic arm starts, removes the culture flask cap, and places it in the cap placement area 601;

[0097] 38. The robotic arm controls the insertion of electrode group 8 into the culture flask, the stirring paddle is started, and the dissolved oxygen value is read as Do5;

[0098] 39. The robotic arm moves the electrode to the cleaning position, the peristaltic pump 507 starts, and the electrode assembly 8 is rinsed with pure water; the robotic arm moves the electrode to the air blowing position, the air pump 502 starts, and the moisture on the electrode assembly 8 is dried through the gas pipeline, in preparation for the analysis of the next sample.

[0099] 40. Repeat steps 37-39 until all samples have been analyzed;

[0100] 41. After all sample analyses are completed, the lifting door 704 at the entrance and exit of the filling area is opened, the conveyor rail is started, the sample is moved from the filling area to the sample transfer area 703, and the sample transport vehicle 4 transports the sample out of the laboratory for further cleaning.

Claims

1. A laboratory-based, fully automated five-day biochemical oxygen demand (BOD) monitoring system, characterized by: It includes a centralized fully automated laboratory analysis system (1), a sample storage incubator (2), an incubator (3), a sample transport vehicle (4), and a pretreatment system; The pretreatment system is located on the side of the laboratory centralized fully automated analysis system (1) and is connected to it via a conveyor belt; The sample storage constant temperature box (2) and the incubator (3) are used for constant temperature storage and incubation of samples, respectively. The sample transport vehicle (4) is used to transport samples between the sample storage constant temperature box (2), the incubator (3) and the pretreatment system.

2. The laboratory-based, fully automated five-day biochemical oxygen demand (BOD) monitoring device according to claim 1, characterized in that: The pretreatment system includes a pretreatment module and a filling module (7). The pretreatment module is divided into a lower part (5) and an upper part (6), with the upper part (6) located above the lower part (5). The lower part (5) of the module is equipped with a reagent box (501), an air pump (502), a pure water tank (503), a dilution inoculation water tank (504), a dilution water tank (505), an injection pump (506), a peristaltic pump (507), and a waste liquid tank (508), and the components are connected by pipelines. The upper part (6) of the module is provided with a bottle cap placement area (601), an aeration robotic arm (602) and an aeration pipeline (603). The aeration pipeline (603) is connected to an air pump (502) and is used to aerate the sample and dilution water.

3. The laboratory-based, fully automated five-day biochemical oxygen demand (BOD) monitoring device according to claim 2, characterized in that: The filling module (7) is connected to the pretreatment module and is divided into a filling area and a sample transfer area (703). The two are connected by a track and a conveyor belt and are separated by a lifting door (704). The filling area is provided with a blank position (701) and a quality control position (702) for filling blank samples and standard samples respectively. A temperature control device is provided at the top of the filling area.

4. The laboratory-based, fully automated five-day biochemical oxygen demand (BOD) monitoring device according to claim 3, characterized in that: The syringe pump (506) is connected to the reagent box (501), the pure water box (503), the dilution inoculation water box (504), the dilution water box (505) and the sample bottle through pipelines, and is used to quantitatively add reagents, dilution water and samples. The dilution inoculation water box (504) and the dilution water box (505) are also connected to the aeration pipeline (603) through pipelines for aeration treatment.

5. The laboratory-based, fully automated five-day biochemical oxygen demand (BOD) monitoring device according to claim 2, characterized in that: The upper part (6) of the module is provided with a robotic arm, which is connected to the robotic arm. The robotic arm moves along the X and Y axes, and the robotic arm moves up and down along the Z axis. The robotic arm is provided with a pipe and a stirring paddle for deep sampling and stirring in the sample bottle. The pipe is connected to the injection pump (506).

6. The laboratory-based, fully automated five-day biochemical oxygen demand (BOD) monitoring device according to claim 1, characterized in that: The sample transport vehicle (4) is equipped with a sample placement platform and a robotic arm. The robotic arm is used to grab or push the sample rack and transfer the sample between the sample storage constant temperature box (2), the incubator (3) and the pretreatment system.

7. The laboratory-based, fully automated five-day biochemical oxygen demand (BOD) monitoring device according to claim 2, characterized in that: Both the pretreatment module and the filling module (7) are equipped with an electrode group (8). The electrode group (8) includes a dissolved oxygen electrode and a stirring paddle, which are used to read the dissolved oxygen concentration and stir and mix the water sample. The electrode group (8) is equipped with a cleaning position and an air blowing position, which are used to clean and dry the electrode, respectively. The cleaning position is connected to the peristaltic pump (507), and the air blowing position is connected to the air pump (502).

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