Athomin compound preparation for preventing and controlling wilt and verticillium wilt in cotton planting and preparation method of athomin compound preparation

Abstract

The invention relates to the technical field of prevention and control of wilt and verticillium wilt, and particularly discloses an athomin compound preparation for preventing and controlling wilt and verticillium wilt in cotton planting and a preparation method of the athomin compound preparation. The compound preparation is prepared from the following raw materials in percentage: 13.0 percent to 15.0 percent of athomin, 1.5 percent to 2.0 percent of cnidium lactone, 4.0 percent to 5.0 percent of antibacterial lipopeptide, 2.0 percent to 2.5 percent of eugenol, 0.5 percent to 0.7 percent of organic silicon synergist, 3.0 percent to 5.0 percent of humic acid, 0.5 percent to 1.0 percent of brassinolide, 1.0 percent to 1.5 percent of chitin oligosaccharide, 8.0 percent to 12 percent of methyl oleate, 5.0 percent to 7.0 percent of N-methyl pyrrolidone and the balance of water. According to the invention, natural components are used for leading, the degradation performance is excellent, the problem of damage of a chemical bactericide to soil ecology is overcome, the influence of diseases is effectively relieved, the yield loss is reduced, the development of cotton fibers is promoted, and the cotton quality is improved. The method provides key technical support for green and sustainable production of cotton, and has remarkable economic, social and ecological benefits.

Description

Technical Field

[0001] This invention belongs to the field of Fusarium wilt control technology, specifically relating to a horseradish compound preparation for controlling Fusarium wilt in cotton cultivation and its preparation method. Background Technology

[0002] Cotton is an important economic crop and strategic resource in my country, and its yield and quality are directly related to agricultural economic development and the security of the textile industry. However, in the process of cotton cultivation, wilt and verticillium wilt have always been major diseases that restrict the development of the industry. Cotton wilt is caused by Fusarium oxysporum cotton-specific strain, while verticillium wilt is caused by Verticillium dahliae. Both are typical soil-borne vascular diseases, characterized by wide spread, severe damage, and great difficulty in prevention and control.

[0003] From the perspective of disease damage, cotton infected with Verticillium wilt suffers from impaired root absorption, resulting in symptoms such as yellowing, wilting, and scorching of leaves. In severe cases, the entire plant dies, leading to reduced yields or even total crop failure. Statistics show that annual yield losses due to Verticillium wilt in major cotton-growing areas of my country can reach 10%-30%, with severely affected fields experiencing yield reductions exceeding 50%. Furthermore, the decline in fiber quality caused by the disease (such as shortened length and reduced strength) further impacts the commercial value of cotton. Even more serious is the fact that the pathogen overwinters in the soil as mycelium and chlamydospores, surviving for 5-10 years. The amount of pathogen in the soil accumulates year by year in continuously cropped fields, creating a "replanting obstacle" and trapping disease control in a vicious cycle.

[0004] Currently, the main control methods for cotton wilt include agricultural control, chemical control, biological control, and disease-resistant breeding, but all of these have obvious limitations.

[0005] Agricultural control measures reduce the number of pathogens through crop rotation and soil disinfection, but the rotation cycle is long (requiring more than 3 years of rotation with non-host crops), which is difficult to adapt to the needs of large-scale planting; soil thermal disinfection or fumigation treatment is costly, energy-intensive, and easily disrupts the soil micro-ecological balance.

[0006] Chemical control remains the mainstream approach, with commonly used agents including carbendazim, hymexazol, and prochloraz. However, long-term, singular use of chemical agents has led to a rapid increase in pathogen resistance, resulting in a gradual decline in control effectiveness. For example, in cotton-growing areas of the Yellow River Basin, the resistance rate of Fusarium to carbendazim has reached over 65%. Furthermore, chemical fungicide residues are a significant problem, polluting not only soil and groundwater but also affecting cotton quality and food safety, contradicting the principles of green agriculture.

[0007] Biological control relies on biocontrol microorganisms such as Bacillus subtilis and Trichoderma and their metabolites, which is environmentally friendly. However, it has problems such as poor speed of action and short duration of effect, making it difficult to meet the control needs during disease outbreaks.

[0008] In summary, the long-term and excessive use of chemical fungicides (such as carbendazim and hymexazol) has led to severe drug resistance in pathogens, resulting in a year-on-year decline in control effectiveness. Simultaneously, pesticide residues damage the soil's microecological environment, kill beneficial microorganisms, affect soil fertility, and pose risks to environmental pollution and agricultural product quality and safety. With increasing environmental awareness and demand for green agricultural products, developing efficient, low-toxicity, and environmentally friendly new control technologies has become an urgent priority. Summary of the Invention

[0009] The purpose of this invention is to provide a horseradish compound preparation for controlling Fusarium wilt in cotton cultivation and its preparation method, so as to solve the problems mentioned in the background art.

[0010] To achieve the above objectives, the present invention provides the following technical solution:

[0011] A horseradish compound preparation for controlling Fusarium wilt in cotton cultivation, the compound preparation being composed of the following raw materials in percentage: horseradish 13.0%-15.0%, osthol 1.5%-2.0%, antimicrobial lipopeptide 4.0%-5.0%, eugenol 2.0%-2.5%, organosilicon synergist 0.5%-0.7%, humic acid 3.0%-5.0%, brassinolide 0.5%-1 0.0%, chitin oligosaccharide 1.0%-1.5%, methyl oleate 8.0%-12%, N-methylpyrrolidone 5.0%-7.0%, castor oil polyoxyethylene ether 8.0%-9.0%, fatty alcohol polyoxyethylene ether phosphate 3.0%-4.0%, glycerol 2.5%-3.0%, silicone defoamer 0.2%-0.3%, Kathon 0.08%-0.10%, balance water.

[0012] Preferably, the compound formulation is composed of the following raw materials in percentage: horseradish extract 14.0%, osthol 1.8%, antimicrobial lipopeptide 4.5%, eugenol 2.2%, organosilicon synergist 0.6%, methyl oleate 10.0%, N-methylpyrrolidone 6.0%, castor oil polyoxyethylene ether 8.5%, fatty alcohol polyoxyethylene ether phosphate 3.5%, glycerol 2.8%, organosilicon defoamer 0.25%, Kathon 0.09%, humic acid 4.0%, brassinolide 0.8%, chitin oligosaccharide 1.2%, and the balance being water.

[0013] A method for preparing a horseradish compound formulation for controlling Fusarium wilt in cotton cultivation includes the following steps;

[0014] S1. Raw material pretreatment:

[0015] Osthol and chitin oligosaccharide were pulverized and passed through an 80-mesh sieve.

[0016] Soak humic acid in a 5% citric acid solution at a solid-liquid ratio of 1:5 and stir to form a paste for later use.

[0017] Preheat methyl oleate and N-methylpyrrolidone in a 30°C water bath;

[0018] Eugenol was filtered through a 0.22 μm organic filter membrane;

[0019] Slowly restore the antimicrobial lipopeptide from 4°C refrigeration to room temperature, and dilute brassinolide with 5 times its volume of water for later use.

[0020] S2, Oil phase preparation:

[0021] Horseradish extract was added to pretreated methyl oleate and N-methylpyrrolidone under stirring conditions of 30-35℃ and 300r / min. After stirring until dissolved, osthol and eugenol were added, and the temperature was raised to 40℃ and stirred to obtain a transparent oil phase.

[0022] S3, Aqueous Phase Preparation:

[0023] Glycerol was added to water at 35-40℃ and 200r / min stirring conditions. After dissolving, castor oil polyoxyethylene ether and fatty alcohol polyoxyethylene ether phosphate were added. The temperature was raised to 40℃ and stirred. Then, pretreated humic acid paste was added and stirred at 400r / min to obtain an aqueous phase.

[0024] S4. Emulsification and homogenization:

[0025] At 40°C, the oil phase is injected into the aqueous phase at a rate of 5 L / min and stirred at 500 r / min to form a primary emulsion. The primary emulsion is then homogenized twice under a pressure of 20 MPa to obtain a homogeneous emulsion.

[0026] S5, Compound:

[0027] The homogenized emulsion was cooled to 30°C, and pretreated antimicrobial lipopeptides and diluted brassinolide were added sequentially. After stirring, chitin oligosaccharides and organosilicon synergists were added and stirred. Finally, organosilicon defoamer and Kathon were added and stirred at 150 r / min. The mixture was then filtered to obtain the horseradish compound preparation.

[0028] Preferably, in step S1, the particle size of the osthol and chitin oligosaccharide after pulverization is ≤180μm.

[0029] Preferably, in step S5, the time interval between adding the antimicrobial lipopeptide and adding Kathon is no less than 10 minutes.

[0030] Preferably, the filter in step S5 is 100 mesh.

[0031] Compared with the prior art, the beneficial effects of the present invention are:

[0032] The compound agent prepared in this invention contains horseradish extract, which can rapidly kill surface pathogens within 24 hours, alleviating wilting symptoms in diseased plants. Osthol and humic acid form a slow-release system with a duration of action exceeding 20 days, extending the effect by 8-10 days compared to existing biological agents. Simultaneously, it significantly enhances the systemic resistance of cotton to Verticillium wilt, reduces the disease recurrence rate in continuously cropped fields, promotes root development and seedling vigor, improves the foundation for stress resistance, and indirectly enhances disease tolerance. Furthermore, this invention does not inhibit cotton seed germination rate or seedling growth; on the contrary, due to the presence of growth-promoting components, it increases the germination rate by 8%-10%. It has no irritating odor during use and is highly safe for applicators.

[0033] Furthermore, this invention is dominated by natural ingredients, has excellent degradation performance, overcomes the damage to soil ecology caused by chemical fungicides, effectively alleviates the impact of diseases, reduces yield loss, and promotes cotton fiber development. It provides key technical support for the green and sustainable production of cotton and has significant economic, social and ecological benefits. Detailed Implementation

[0034] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0035] Example 1:

[0036] A horseradish compound preparation for controlling Fusarium wilt in cotton cultivation.

[0037] The compound formulation is composed of the following raw materials in percentage: horseradish extract 14.0%, osthol 1.8%, antimicrobial lipopeptide 4.5%, eugenol 2.2%, organosilicon synergist 0.6%, methyl oleate 10.0%, N-methylpyrrolidone 6.0%, castor oil polyoxyethylene ether 8.5%, fatty alcohol polyoxyethylene ether phosphate 3.5%, glycerol 2.8%, organosilicon defoamer 0.25%, Kathon 0.09%, humic acid 4.0%, brassinolide 0.8%, chitin oligosaccharide 1.2%, and the balance being water.

[0038] The preparation process includes the following steps:

[0039] S1. Raw material pretreatment

[0040] Solid raw material processing: 1.8 kg of osthol crystals and 1.2 kg of chitin oligosaccharide powder were crushed through an 80-mesh sieve to ensure that the particle size was ≤180 μm and collected for later use.

[0041] Humic acid pretreatment: Take 4.0 kg of humic acid, add 5% citric acid solution (at a solid-liquid ratio of 1:5, i.e., 20 kg of solution), stir and soak for 2 hours until a uniform paste is formed, then seal and store away from light.

[0042] Liquid raw material processing: 10.0 kg of methyl oleate and 6.0 kg of N-methylpyrrolidone were placed in a 30°C water bath and preheated for 30 minutes; 2.2 kg of eugenol was filtered through a 0.22 μm organic filter membrane to remove impurities; 4.5 kg of antimicrobial lipopeptides were taken out of the 4°C refrigerated environment and allowed to stand at room temperature for 1 hour to recover to 25°C; 0.8 kg of brassinolide was diluted with 4 kg of water (5 times the volume) and stirred evenly for later use.

[0043] S2, Oil Phase Preparation

[0044] Add preheated methyl oleate and N-methylpyrrolidone to a 50L stainless steel reactor, turn on the stirrer, and set the temperature to 32℃ and the speed to 300r / min.

[0045] Slowly add 14.0 kg of horseradish extract and stir continuously for 15 minutes until completely dissolved. The solution will be pale yellow and transparent.

[0046] Add the pulverized osthol and the filtered eugenol in sequence, heat to 40°C, and stir for 20 minutes to obtain a homogeneous and transparent oil phase system. Turn off the heating and keep warm for later use.

[0047] S3, Aqueous Phase Preparation

[0048] Add 42.76 kg of tap water to a 100 L mixing tank, turn on the mixer, and set the temperature to 37 °C and the speed to 200 r / min.

[0049] Add 2.8 kg of glycerol and stir for 5 minutes until completely dissolved. Then add 8.5 kg of castor oil polyoxyethylene ether and 3.5 kg of fatty alcohol polyoxyethylene ether phosphate in sequence. Heat to 40°C and stir for 15 minutes until the solution is slightly milky white.

[0050] Add the pretreated humic acid paste, increase the speed to 400 r / min, and stir for 30 minutes until there is no obvious particle precipitation, thus obtaining a homogeneous aqueous system.

[0051] S4, Emulsification and Homogenization

[0052] The oil phase system was slowly injected into the aqueous phase mixing tank at a rate of 5 L / min using a peristaltic pump, while the aqueous phase stirring speed was maintained at 500 r / min. After the oil phase was injected, stirring was continued for 30 minutes to form a light brown colostrum.

[0053] Transfer the colostrum to a high-pressure homogenizer, set the pressure to 20 MPa, and homogenize twice (5 minutes each time). Take a sample to observe the fineness of the emulsion and ensure that there are no visible particles.

[0054] S5, Compounding and Post-processing

[0055] Cool the homogenized emulsion to 30°C, add the antimicrobial lipopeptide that has been brought back to room temperature, stir for 15 minutes (300 rpm), then add the diluted brassinolide and continue stirring for 10 minutes.

[0056] Add chitin oligosaccharide and organosilicon synergist in sequence, stir for 20 minutes until completely dissolved, and the system exhibits a homogeneous flow dynamic.

[0057] After a 15-minute interval (meeting the requirement of ≥10 minutes), add the silicone defoamer and Kathon, slow down the speed to 150 r / min, and stir for 10 minutes to eliminate foam.

[0058] The preparation was filtered through a 100-mesh filter to remove any possible mechanical impurities, resulting in the finished horseradish compound preparation, which was then bottled and sealed in a 20L brown plastic drum.

[0059] Example 2:

[0060] A horseradish compound preparation for controlling Fusarium wilt in cotton cultivation.

[0061] The compound formulation consists of the following raw materials in percentage: horseradish extract 14.0%, osthol 1.8%, antimicrobial lipopeptide 4.5%, eugenol 2.2%, organosilicon synergist 0.6%, humic acid 4.0%, brassinolide 0.8%, chitin oligosaccharide 1.2%, methyl oleate 10.0%, N-methylpyrrolidone 6.0%, castor oil polyoxyethylene ether 8.5%, fatty alcohol polyoxyethylene ether phosphate 3.5%, glycerol 2.8%, organosilicon defoamer 0.25%, Kathon 0.09%, and water as the balance.

[0062] Preparation method of horseradish compound formulation for controlling Fusarium wilt in cotton cultivation:

[0063] S1. Raw material pretreatment

[0064] Solid raw material processing: 3.6 kg of osthol crystals and 2.4 kg of chitin oligosaccharide powder were crushed separately using an 80-mesh sieve to ensure that the particle size was ≤180 μm, and then collected for later use.

[0065] Humic acid pretreatment: Take 8.0 kg of humic acid, add 5% citric acid solution (according to a solid-liquid ratio of 1:5, i.e., 40 kg of solution), stir and soak for 2 hours until a uniform paste is formed, seal and store away from light.

[0066] Liquid raw material processing: 20.0 kg of methyl oleate and 12.0 kg of N-methylpyrrolidone were preheated in a 30°C water bath for 30 minutes; 4.4 kg of eugenol was filtered through a 0.22 μm organic filter membrane to remove impurities; 9.0 kg of antimicrobial lipopeptides were taken out of the 4°C refrigerated environment, allowed to stand at room temperature for 1 hour, and then restored to 25°C; 1.6 kg of brassinolide was diluted with 8 kg of water (5 times the volume), stirred evenly, and set aside.

[0067] S2, Oil Phase Preparation

[0068] Add preheated methyl oleate and N-methylpyrrolidone to a 100L stainless steel reactor, turn on the stirrer, and set the temperature to 33℃ and the speed to 300r / min.

[0069] Slowly add 28.0 kg of horseradish extract and stir continuously for 15 minutes until completely dissolved, resulting in a pale yellow and transparent solution.

[0070] Add the pulverized osthol and filtered eugenol in sequence, heat to 40°C, and stir at the current speed for 20 minutes to obtain a homogeneous and transparent oil phase system. Turn off the heating and keep warm for later use.

[0071] S3, Aqueous Phase Preparation

[0072] Add 85.52 kg of tap water to a 200 L mixing tank, turn on the mixer, and set the temperature to 38 °C and the speed to 200 r / min.

[0073] Add 5.6 kg of glycerol and stir for 5 minutes until completely dissolved. Then add 17.0 kg of castor oil polyoxyethylene ether and 7.0 kg of fatty alcohol polyoxyethylene ether phosphate in sequence. Heat to 40°C and stir for 15 minutes until the solution is slightly milky white.

[0074] Add the pretreated humic acid paste, increase the rotation speed to 400 r / min, stir for 30 minutes until there is no obvious particle precipitation, and obtain a homogeneous aqueous system.

[0075] S4, Emulsification and Homogenization

[0076] The oil phase system was slowly injected into the aqueous phase mixing tank at a rate of 5 L / min using a peristaltic pump, while the aqueous phase stirring speed was maintained at 500 r / min. After the oil phase was injected, stirring was continued for 30 minutes to form a light brown colostrum.

[0077] Transfer the colostrum to a high-pressure homogenizer, set the pressure to 20 MPa, and homogenize twice (5 minutes each time). Take a sample to observe the fineness of the emulsion and ensure that there are no visible particles.

[0078] S5, Compounding and Post-processing

[0079] Cool the homogenized emulsion to 30°C, add the antimicrobial lipopeptide that has been brought back to room temperature, stir for 15 minutes (300 rpm), then add the diluted brassinolide and continue stirring for 10 minutes.

[0080] Add chitin oligosaccharide and organosilicon synergist in sequence, stir for 20 minutes until completely dissolved, and the system exhibits a homogeneous flow dynamic.

[0081] After an interval of 18 minutes (meeting the requirement of ≥10 minutes), add the silicone defoamer and Kathon, slow down the speed to 150 r / min, and stir for 10 minutes to eliminate foam.

[0082] The preparation was filtered through a 100-mesh filter to remove any possible mechanical impurities, resulting in the finished horseradish compound preparation, which was then bottled and sealed in a 20L brown plastic drum.

[0083] Example 3:

[0084] A horseradish compound preparation for controlling Fusarium wilt in cotton cultivation:

[0085] The compound formulation consists of the following raw materials in percentage:

[0086] Horseradish extract 13.5%, osthol 1.7%, antimicrobial lipopeptide 4.2%, eugenol 2.3%, organosilicon synergist 0.65%, humic acid 3.5%, brassinolide 0.7%, chitin oligosaccharide 1.3%, methyl oleate

[0087] 9.0%, N-methylpyrrolidone 6.5%, castor oil polyoxyethylene ether 8.2%, fatty alcohol polyoxyethylene ether phosphate 3.2%, glycerol 2.6%, silicone defoamer 0.22%, Kathon 0.095%, water balance.

[0088] The preparation process includes the following steps:

[0089] S1. Raw material pretreatment

[0090] Solid raw material processing: 2.55 kg of osthol crystals and 1.95 kg of chitin oligosaccharide powder were crushed separately using an 80-mesh sieve to ensure that the particle size was ≤180 μm, and collected for later use.

[0091] Humic acid pretreatment: Take 5.25 kg of humic acid, add 5% citric acid solution (solid-liquid ratio 1:5, i.e., 26.25 kg of solution), stir and soak for 2 hours until a uniform paste is formed, seal and store away from light (in the dry environment of Northwest China, it is necessary to cover with plastic wrap to prevent water loss).

[0092] Liquid raw material processing: 13.5 kg of methyl oleate and 9.75 kg of N-methylpyrrolidone were placed in a 30°C water bath and preheated for 30 minutes; 3.45 kg of eugenol was filtered through a 0.22 μm organic filter membrane to remove impurities; 6.3 kg of antimicrobial lipopeptides were taken out of the 4°C refrigerator and allowed to stand at room temperature for 1 hour to recover to 25°C; 1.05 kg of brassinolide was diluted with 5.25 kg of water (5 times the volume) and stirred evenly for later use.

[0093] S2, Oil Phase Preparation

[0094] Add preheated methyl oleate and N-methylpyrrolidone to an 80L stainless steel reactor, turn on the stirrer, and set the temperature to 34℃ and the speed to 300r / min (the ambient temperature in Northwest China is low, so the initial temperature needs to be increased appropriately).

[0095] Slowly add 20.25 kg of horseradish extract and stir continuously for 15 minutes until completely dissolved. The solution will be pale yellow and transparent.

[0096] Add pulverized osthol and filtered eugenol in sequence, heat to 40°C, and stir for 20 minutes to obtain a homogeneous and transparent oil phase system. Turn off the heating and keep warm for later use.

[0097] S3, Aqueous Phase Preparation

[0098] Add 67.2325 kg of groundwater (pH 7.8, total hardness 350 mg / L) to a 150 L mixing tank, start the mixer, and set the temperature to 39 °C and the speed to 200 r / min.

[0099] Add 3.9 kg of glycerol and stir for 5 minutes until dissolved. Then add 12.3 kg of castor oil polyoxyethylene ether and 4.8 kg of fatty alcohol polyoxyethylene ether phosphate (to enhance hard water resistance) in sequence. Heat to 40°C and stir for 15 minutes until the solution is slightly milky white.

[0100] Add the pretreated humic acid paste, increase the speed to 400 r / min, and stir for 30 minutes until there is no obvious particle precipitation, thus obtaining a homogeneous aqueous system.

[0101] S4, Emulsification and Homogenization

[0102] The oil phase was injected into the aqueous phase mixing tank at a rate of 5 L / min using a peristaltic pump, while the aqueous phase stirring speed was maintained at 500 r / min. After the oil phase was injected, stirring was continued for 30 minutes to form a light brown colostrum.

[0103] Transfer the colostrum to a high-pressure homogenizer, set the pressure to 20 MPa, and homogenize twice (5 minutes each time). Take a sample to observe the fineness of the emulsion and ensure that there are no visible particles (the water quality in the Northwest is relatively hard, so the homogenization time needs to be extended by 1 minute).

[0104] S5, Compounding and Post-processing

[0105] Cool the homogenized emulsion to 30°C, add the antimicrobial lipopeptide that has been brought back to room temperature, stir for 15 minutes (300 rpm), then add the diluted brassinolide and continue stirring for 10 minutes.

[0106] Add chitin oligosaccharide and organosilicon synergist in sequence, stir for 20 minutes until completely dissolved, and the system exhibits a homogeneous flow dynamic.

[0107] After an interval of 20 minutes (meeting the requirement of ≥10 minutes), add the silicone defoamer and Kathon, slow down the speed to 150 r / min, and stir for 10 minutes to eliminate foam.

[0108] The preparation was filtered through a 100-mesh filter to remove mechanical impurities, yielding the finished horseradish compound preparation, which was then bottled into 20L brown plastic drums and sealed.

[0109] In summary, the present invention takes the horseradish compound preparation of Example 1 as an example.

[0110] The specific usage instructions are as follows:

[0111] (I) Duration of medication

[0112] Seedling stage prevention: Apply pesticides when cotton has 4-6 leaves. At this time, the cotton root system begins to develop rapidly, which is a critical period for pathogen infection. Applying pesticides in advance can build a protective barrier for the root system and reduce the risk of disease.

[0113] Control during the flowering and boll-forming stage: Apply pesticides from the early flowering stage to the boll-forming stage of cotton. During this stage, cotton is in vigorous vegetative and reproductive growth, has a high demand for nutrients, and is susceptible to pathogens. Applying pesticides can control the spread of diseases and ensure the quality of boll formation.

[0114] Emergency control measures in the early stages of the disease: When sporadic diseased plants (yellowing and wilting of leaves) appear in the field, pesticides should be applied immediately, and the pesticides should be applied twice (with an interval of 7-10 days) to curb the spread of the disease.

[0115] (II) Application Method

[0116] Depending on the planting patterns and disease prevalence in different cotton-growing areas, the following two application methods can be used:

[0117] Foliar spraying + root irrigation

[0118] Foliar spraying: Dilute the formulation with water at a ratio of 1:500 (100-120 mL of formulation per acre, diluted with 50-60 kg of water), and use a backpack sprayer or motorized sprayer to spray evenly on both sides of the cotton leaves, focusing on the middle and lower leaves and the base of the stem.

[0119] Root irrigation treatment: Dilute with water at a ratio of 1:300 (100-120 mL of the preparation per acre, diluted with 30-36 kg of water). Use a drip irrigation system or a manual root irrigation device to slowly irrigate the soil around the cotton plant roots within a 10 cm radius, ensuring that the solution penetrates into the root distribution layer (10-15 cm deep).

[0120] Drip irrigation application

[0121] When the drip irrigation system is running, the preparation is applied through the fertilizer tank at a ratio of 1:800 with the drip irrigation water, with a dosage of 120-150mL per acre, so that the liquid is evenly distributed in the rhizosphere soil with the water flow, which is suitable for the water-saving irrigation needs of arid areas.

[0122] (III) Dosage and Frequency of Medication

[0123] Routine prevention: Apply 100 mL per acre each time, once during the seedling stage and once during the flowering and boll-forming stage, for a total of 2 times throughout the year.

[0124] For severe disease control: use 120mL per mu each time, apply once each during the seedling stage, early flowering stage, and flowering and boll-forming stage. If the disease is severe, an additional application can be added between the two applications, with a maximum of 4 applications per year.

[0125] III. Precautions for Use

[0126] Application conditions

[0127] Choose a sunny, windless or lightly windy day for application, with the temperature ideally between 15-30℃. Avoid applying the pesticide on rainy days, in high temperatures and strong sunlight (temperature > 35℃), or in windy days (wind force > level 3) to prevent the pesticide from running off or evaporating too quickly. If it rains within 4 hours after foliar spraying, re-spray. If there is drought after root irrigation, water appropriately to ensure the pesticide penetrates the soil.

[0128] Pharmaceutical preparation

[0129] When diluting, first pour the formulation into a small amount of water and stir well, then add the remaining water and mix thoroughly. Avoid pouring the formulation directly into a large amount of water, which may lead to uneven dispersion. In areas with high water hardness in Northwest China, 0.1% of a chelating agent (such as EDTA) can be added during dilution to enhance the stability of the solution.

[0130] This invention can be used in combination with non-alkaline insecticides and foliar fertilizers (such as potassium dihydrogen phosphate), but it cannot be used in combination with alkaline agents such as Bordeaux mixture and lime sulfur.

[0131] The preparation should be stored in a cool, dry, well-ventilated, and dark warehouse, away from fire and areas accessible to children; avoid violent collisions and exposure to sunlight during transportation, and keep the temperature between -5℃ and 35℃. The shelf life is 12 months.

[0132] In summary, the horseradish compound formulation of this invention achieves multiple core values ​​through scientific formulation and process design, which are mainly reflected in the following aspects.

[0133] It has a highly efficient and synergistic bactericidal effect. It forms a multi-target bactericidal system with horseradish extract, osthol, antimicrobial lipopeptides and eugenol, which improves the overall inhibition rate of the pathogen of cotton wilt and achieves a combination of 24-hour rapid bactericidal effect and 20-day long-term control, thus reducing the number of times the medicine is used during the growing season.

[0134] Secondly, it enhances systemic disease resistance. Chitin oligosaccharides and brassinolide dually activate crop immunity, reducing the recurrence rate of diseases in continuously cropped fields. Humic acid and adjuvants work together to promote root growth and seedling development, increase root fresh weight, and accelerate the recovery of diseased plants, thus solving the problem that traditional agents only treat the symptoms and not the root cause.

[0135] Third, it is environmentally friendly and safe, with natural ingredients accounting for 18%, a degradation rate of over 90% within 30 days, and minimal impact on soil microorganisms; it is low-toxicity to bees and earthworms, and increases cotton germination rate by 8%-10%, meeting the standards for green food production.

[0136] Fourth, the formulation is stable and applicable. The compound solvent and emulsion system ensure that there is no stratification from -5℃ to 40℃, and the shelf life at room temperature is up to 12 months. The organosilicon synergist is suitable for foliar spraying (increases droplet spreading by 50%) and root irrigation, and the operation is convenient without secondary dilution.

[0137] It should be understood that numerous specific implementation decisions can be made during the development of any practical implementation, such as in any engineering or design project. Such development efforts may be complex and time-consuming, but for those skilled in the art who benefit from this disclosure, the development effort will be a routine work of design, manufacturing, and production without requiring much experimentation.

[0138] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit it. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, and all such modifications or substitutions should be covered within the scope of the claims of the present invention.

Claims

1. A horseradish compound preparation for controlling Fusarium wilt in cotton cultivation, characterized in that, The compound formulation is composed of the following raw materials in percentage: horseradish extract 13.0%-15.0%, osthol 1.5%-2.0%, antibacterial lipopeptide 4.0%-5.0%, eugenol 2.0%-2.5%, organosilicon synergist 0.5%-0.7%, humic acid 3.0%-5.0%, brassinolide 0.5%-1.0%, chitin oligosaccharide 1.0%-1.5%, methyl oleate 8.0%-12%, N-methylpyrrolidone 5.0%-7.0%, castor oil polyoxyethylene ether 8.0%-9.0%, fatty alcohol polyoxyethylene ether phosphate 3.0%-4.0%, glycerol 2.5%-3.0%, organosilicon defoamer 0.2%-0.3%, Kathon 0.08%-0.10%, and the balance being water.

2. The horseradish compound preparation for controlling Fusarium wilt in cotton cultivation according to claim 1, characterized in that, The compound formulation is composed of the following raw materials in percentage: horseradish extract 14.0%, osthol 1.8%, antimicrobial lipopeptide 4.5%, eugenol 2.2%, organosilicon synergist 0.6%, methyl oleate 10.0%, N-methylpyrrolidone 6.0%, castor oil polyoxyethylene ether 8.5%, fatty alcohol polyoxyethylene ether phosphate 3.5%, glycerol 2.8%, organosilicon defoamer 0.25%, Kathon 0.09%, humic acid 4.0%, brassinolide 0.8%, chitin oligosaccharide 1.2%, and the balance being water.

3. The method for preparing a horseradish compound preparation for controlling Fusarium wilt in cotton cultivation according to claim 1, characterized in that: Includes the following steps; S1. Raw material pretreatment: Osthol and chitin oligosaccharide were pulverized and passed through an 80-mesh sieve. Soak humic acid in a 5% citric acid solution at a solid-liquid ratio of 1:5 and stir to form a paste for later use. Preheat methyl oleate and N-methylpyrrolidone in a 30°C water bath; Eugenol was filtered through a 0.22 μm organic filter membrane; Slowly restore the antimicrobial lipopeptide from 4°C refrigeration to room temperature, and dilute brassinolide with 5 times its volume of water for later use. S2, Oil phase preparation: Horseradish extract was added to pretreated methyl oleate and N-methylpyrrolidone under stirring conditions of 30-35℃ and 300r / min. After stirring until dissolved, osthol and eugenol were added, and the temperature was raised to 40℃ and stirred to obtain a transparent oil phase. S3, Aqueous Phase Preparation: Glycerol was added to water at 35-40℃ and 200r / min stirring conditions. After dissolving, castor oil polyoxyethylene ether and fatty alcohol polyoxyethylene ether phosphate were added. The temperature was raised to 40℃ and stirred. Then, pretreated humic acid paste was added and stirred at 400r / min to obtain an aqueous phase. S4. Emulsification and homogenization: At 40°C, the oil phase is injected into the aqueous phase at a rate of 5 L / min and stirred at 500 r / min to form a primary emulsion. The primary emulsion is then homogenized twice under a pressure of 20 MPa to obtain a homogeneous emulsion. S5, Compound: The homogenized emulsion was cooled to 30°C, and pretreated antimicrobial lipopeptides and diluted brassinolide were added sequentially. After stirring, chitin oligosaccharides and organosilicon synergists were added and stirred. Finally, organosilicon defoamer and Kathon were added and stirred at 150 r / min. The mixture was then filtered to obtain the horseradish compound preparation.

4. The method for preparing a horseradish compound preparation for controlling Fusarium wilt in cotton cultivation according to claim 3, characterized in that: In step S1, the particle size of the osthol and chitin oligosaccharide after pulverization is ≤180μm.

5. The method for preparing a horseradish compound preparation for controlling Fusarium wilt in cotton cultivation according to claim 3, characterized in that: In step S5, the time interval between adding the antimicrobial lipopeptide and adding Kathon is no less than 10 minutes.

6. The method for preparing a horseradish compound preparation for controlling Fusarium wilt in cotton cultivation according to claim 3, characterized in that: The filter used in step S5 is 100 mesh.