Hydrogen-rich water compound polysaccharide bactericide for preventing and treating cherry brown rot as well as preparation method and application of hydrogen-rich water compound polysaccharide bactericide

Abstract

The invention discloses a hydrogen-rich water compound polysaccharide bactericide for preventing and treating cherry brown rot and a preparation method and application thereof.The hydrogen-rich water compound polysaccharide bactericide is prepared from chitosan, octyl glucoside, decyl glucoside and hydrogen-rich water through a specific compounding technology, and experimental results prove that the hydrogen-rich water compound polysaccharide bactericide has the advantages that the content of the hydrogen-rich water compound polysaccharide bactericide is increased; the hydrogen-rich water compound polysaccharide bactericide for preventing and treating the cherry brown rot can remarkably improve hardness and soluble solid content of cherry fruits, reduce indexes such as respiration rate and weight loss rate of the cherry fruits and reduce morbidity of the cherry brown rot, the fruits are bright in color and luster, double effects of disease prevention and control and quality improvement are achieved, and the bactericide is green, environmentally friendly and suitable for popularization and application. Good application prospects are realized.

Description

Technical Field

[0001] This invention relates to the field of agricultural fungicide technology, specifically to a hydrogen-rich water-based composite polysaccharide fungicide for controlling cherry brown rot, its preparation method, and its application. Background Technology

[0002] Cherry brown rot is one of the most serious diseases affecting cherry cultivation, primarily damaging flowers, leaves, twigs, and fruit. Especially during fruit ripening, it often causes extensive fruit rot, severely impacting cherry yield and quality, resulting in significant economic losses for fruit growers. Currently, the control of cherry brown rot mainly relies on chemical fungicides, such as carbendazim and thiophanate-methyl. However, long-term and excessive use of chemical fungicides not only easily leads to drug resistance in pathogens, reducing control effectiveness, but also results in pesticide residues, harming human health and damaging the ecological environment.

[0003] Polysaccharide fungicides, such as chitosan, have received widespread attention in the field of agricultural disease control due to their advantages of being natural, environmentally friendly, and harmless to humans. Chitosan can induce disease resistance in plants and has a direct inhibitory effect on pathogens. Alkyl polysaccharides are green surfactants that can enhance the adhesion and penetration of fungicides on plant surfaces. Hydrogen-rich water has strong reducing and antioxidant properties, and its application in agriculture has shown a certain promoting effect on plant growth and stress resistance. However, there are currently no reports on the use of alkyl polysaccharides, chitosan, and hydrogen-rich water in the control of cherry brown rot, and on the related technologies for systematically studying their compounding process, spraying techniques, and control effects. Summary of the Invention

[0004] This invention aims to provide a hydrogen-rich water-based compound polysaccharide fungicide for the prevention and control of cherry brown rot, its preparation method, and its application. By optimizing the compounding process and determining the scientific spraying time and method, the incidence of cherry brown rot can be effectively reduced, while improving the quality of cherry fruit, thus solving the problems existing in current chemical control methods.

[0005] The objective of this invention is achieved through the following technical solution:

[0006] A hydrogen-rich water-based polysaccharide fungicide for controlling cherry brown rot is prepared from chitosan, octyl glucoside, decyl glucoside, and hydrogen-rich water. The chitosan comprises 7.2-10.8 wt% of the total weight of the hydrogen-rich water-based polysaccharide fungicide, the octyl glucoside comprises 8-12 wt% of the total weight ...

[0007] The aforementioned hydrogen-rich water-based polysaccharide fungicide for controlling cherry brown rot comprises chitosan accounting for 8.1-9.9 wt% of the total weight of the hydrogen-rich water-based polysaccharide fungicide, octyl glucoside accounting for 9-11 wt% of the total weight of the hydrogen-rich water-based polysaccharide fungicide, decyl glucoside accounting for 9-11 wt% of the total weight of the hydrogen-rich water-based polysaccharide fungicide, and hydrogen-rich water accounting for 73.9-68.1 wt% of the total weight of the hydrogen-rich water-based polysaccharide fungicide.

[0008] Specifically, in the aforementioned hydrogen-rich water-based polysaccharide fungicide for controlling cherry brown rot, chitosan accounts for 9 wt% of the total weight of the hydrogen-rich water-based polysaccharide fungicide, octyl glucoside accounts for 10 wt% of the total weight of the hydrogen-rich water-based polysaccharide fungicide, decyl glucoside accounts for 10 wt% of the total weight of the hydrogen-rich water-based polysaccharide fungicide, and hydrogen-rich water accounts for 71 wt% of the total weight of the hydrogen-rich water-based polysaccharide fungicide.

[0009] The aforementioned method for preparing the hydrogen-rich water-based polysaccharide fungicide for controlling cherry brown rot is carried out according to the following steps:

[0010] (1) Preparation of chitosan solution: Slowly add chitosan to citric acid or lactic acid solution. The amount of citric acid or lactic acid solution is 1.5% of the total weight of chitosan. In a sealed magnetic reactor, stir at 300-400 rpm for 20-30 minutes until completely transparent. Then adjust the pH to 4.5-5.2 with citric acid or lactic acid to prevent gelation. The chitosan solution is then obtained.

[0011] (2) Preparation of hydrogen-rich water: Using a high-pressure hydrogen dissolving device, purified hydrogen gas with a volume concentration of 99.99% is dissolved at room temperature at a rate of 200-400 mL / min. -1 The hydrogen is released at a rate of 2-4L into 2-4h for 2-4h until the dissolved hydrogen concentration is ≥1.5ppm, thus producing hydrogen-rich water.

[0012] (3) Preparation of alkyl polysaccharide pre-solution: After mixing octyl glucoside and decyl glucoside, add hydrogen-rich water obtained in step (2). The weight of the added hydrogen-rich water is 2.5 times that of the mixture of octyl glucoside and decyl glucoside. Stir at 200-300 rpm for 15-20 min in a sealed magnetic reactor to obtain alkyl polysaccharide pre-solution.

[0013] (4) Preparation of bactericide: The chitosan solution obtained in step (1) and the alkyl polysaccharide pre-solution obtained in step (3) are slowly mixed and stirred at 300-400 rpm for 20-30 min in a sealed magnetic reactor to obtain the mother liquor; under stirring, the hydrogen-rich water obtained in step (2) is slowly added to the mother liquor so that the total amount of hydrogen-rich water accounts for 76.8-65.2% of the total weight of the mother liquor. After stirring for 10-15 min, the mixture is immediately sealed and filled to obtain the hydrogen-rich water composite polysaccharide bactericide.

[0014] In step (1) above, the chitosan solution is prepared by slowly adding chitosan to a citric acid or lactic acid solution. The amount of citric acid or lactic acid solution is 1.5% of the total weight of chitosan. In a sealed magnetic reactor, the mixture is stirred at 350 rpm for 25 minutes until it is completely transparent. Then, the pH is adjusted to 4.5-5.2 with citric acid or lactic acid to prevent gelation, thus obtaining the chitosan solution.

[0015] In step (2) above, hydrogen-rich water is prepared by using a high-pressure hydrogen dissolving device to dissolve purified hydrogen gas with a volume concentration of 99.99% at room temperature at a rate of 300 mL / min. -1 The hydrogen is released into 3L of distilled water at a rate that lasts for 3 hours until the dissolved hydrogen concentration is ≥1.5ppm, thus producing hydrogen-rich water.

[0016] In step (3) above, the alkyl polysaccharide pre-solution is prepared by mixing octyl glucoside and decyl glucoside and adding hydrogen-rich water prepared in step (2). The weight of the added hydrogen-rich water is 2.5 times that of the mixture of octyl glucoside and decyl glucoside. The mixture is stirred at 250 rpm for 18 minutes in a sealed magnetic reactor to obtain the alkyl polysaccharide pre-solution.

[0017] In step (4) above, the bactericide is prepared by slowly mixing the chitosan solution obtained in step (1) and the alkyl polysaccharide pre-solution obtained in step (3) in a sealed magnetic reactor and stirring at 350 rpm for 25 min to obtain the mother liquor. While stirring, the hydrogen-rich water obtained in step (2) is slowly added to the mother liquor so that the total amount of hydrogen-rich water accounts for 71% of the total weight of the mother liquor. After stirring for 12 min, the mixture is immediately sealed and filled to obtain the hydrogen-rich water composite polysaccharide bactericide.

[0018] The aforementioned hydrogen-rich water-compound polysaccharide fungicide is used for the treatment and prevention of cherry brown rot. The spraying method of the hydrogen-rich water-compound polysaccharide fungicide is as follows: after diluting the high-concentration hydrogen-rich water-compound polysaccharide fungicide by 1000-1500 times, the first spray is carried out 7-10 days after cherry blossom fall, the second spray is carried out after an interval of 10-15 days, and the third spray is carried out during the fruit enlargement period; a high-pressure spraying device is used to evenly spray the fungicide on the leaves, fruits and branches of the cherry tree, the spray droplet size is controlled at 100-300μm, and the spraying amount is 30-50L per acre.

[0019] The aforementioned method for spraying the hydrogen-rich water-compound polysaccharide fungicide is as follows: dilute the high-concentration hydrogen-rich water-compound polysaccharide fungicide 1000 times, and spray it for the first time 10 days after cherry blossoms fall, spray it for the second time after an interval of 15 days, and spray it for the third time during the fruit enlargement period; use high-pressure spraying equipment to evenly spray the fungicide on the leaves, fruits and branches of the cherry trees, control the spray droplet size to 300μm, and spray 50L per acre.

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] 1. Highly effective disease control: The hydrogen-rich water compound polysaccharide fungicide of this invention can significantly reduce the incidence of cherry brown rot through the synergistic effect of alkyl polysaccharide glycosides (composed of octyl glucoside and decyl glucoside in a 1:1 mass ratio), chitosan and hydrogen-rich water. Field trials have verified that the incidence of disease is effectively reduced, which is significantly better than the control effect of traditional chemical fungicides, and it is not easy to produce drug resistance.

[0022] 2. Improve fruit quality: After spraying the hydrogen-rich water-based polysaccharide fungicide of this invention, the firmness of the fruit increases, which is beneficial for fruit picking, transportation and storage; the soluble solids content increases, which improves the sweetness of the fruit; the fruit respiration rate and weight loss rate decrease and the brightness L* value increases, making the fruit color more vibrant, reducing the incidence of cherry brown rot and improving the commercial value of the fruit.

[0023] 3. The hydrogen-rich water-based polysaccharide bactericide of the present invention has been verified through field trials. In Experiment D, a high-pressure spraying device was used to control the droplet size to about 300 μm. The spraying amount was 50 L per acre. The first spraying was carried out 10 days after cherry blossom fall, the second spraying was carried out 15 days later, and the third spraying was carried out during the fruit enlargement period. This spraying method showed the best performance in maintaining fruit firmness, increasing TSS content, reducing respiration rate and weight loss rate.

[0024] 4. Green and environmentally friendly: The components of the hydrogen-rich water composite polysaccharide bactericide of this invention are all natural or environmentally friendly substances, with no pesticide residues, harmless to human health, and environmentally friendly, meeting the requirements of modern agricultural green development.

[0025] 5. The hydrogen-rich water-based polysaccharide bactericide of this invention can significantly improve the firmness and soluble solids content of cherry fruits, and reduce indicators such as respiration rate and weight loss rate of cherry fruits, thereby reducing the incidence of cherry brown rot. In addition, the fruit has a bright color, achieving the dual effect of disease control and quality improvement. It is green and environmentally friendly and has good application prospects. Attached Figure Description

[0026] Figure 1 This is a comparison chart of the results of field spraying. Detailed Implementation

[0027] The present invention will be further described below with reference to embodiments, but these embodiments are not intended to limit the scope of the invention.

[0028] Example 1:

[0029] Material ratio: 9wt% chitosan, 10wt% octyl glucoside, 10wt% decyl glucoside and 71wt% hydrogen-rich water.

[0030] Preparation method of hydrogen-rich water complex polysaccharide bactericide:

[0031] (1) Preparation of chitosan solution: Slowly add 9g of chitosan to 0.135g of citric acid or lactic acid solution. The amount of citric acid or lactic acid solution is 1.5% of the total weight of chitosan. In a sealed magnetic reactor, stir at 350rpm for 25min until completely transparent. Then adjust the pH to 4.5-5.2 with citric acid or lactic acid to prevent gelation. The chitosan solution is then obtained.

[0032] (2) Preparation of hydrogen-rich water: Purified hydrogen gas with a volume concentration of 99.99% was dissolved in a high-pressure hydrogen dissolving device at room temperature at a rate of 300 mL / min. -1 The hydrogen is released into 3L of distilled water at a rate that lasts for 3 hours until the dissolved hydrogen concentration is ≥1.5ppm, thus producing hydrogen-rich water.

[0033] (3) Preparation of alkyl polysaccharide pre-solution: Mix 10g octyl glucoside and 10g decyl glucoside, and add 50g of hydrogen-rich water prepared in step (2). The weight of the added hydrogen-rich water is 2.5 times that of the mixture of octyl glucoside and decyl glucoside. Stir at 250rpm for 18min in a sealed magnetic reactor to obtain the alkyl polysaccharide pre-solution.

[0034] (4) Preparation of bactericide: The chitosan solution obtained in step (1) and the alkyl polysaccharide pre-solution obtained in step (3) are slowly mixed and stirred at 350 rpm for 25 min in a sealed magnetic reactor to obtain the mother liquor; under stirring, the hydrogen-rich water obtained in step (2) is slowly added to the mother liquor so that the total amount of hydrogen-rich water accounts for 71% of the total weight of the mother liquor. After stirring for 12 min, the mixture is immediately sealed and filled to obtain the hydrogen-rich water composite polysaccharide bactericide.

[0035] Example 2:

[0036] Material ratio: 9wt% chitosan, 10wt% octyl glucoside, 10wt% decyl glucoside and 71wt% hydrogen-rich water.

[0037] Preparation method of hydrogen-rich water complex polysaccharide bactericide:

[0038] (1) Preparation of chitosan solution: Slowly add 9g of chitosan to 0.135g of citric acid or lactic acid solution. The amount of citric acid or lactic acid solution is 1.5% of the total weight of chitosan. Stir at 300rpm for 20min in a sealed magnetic reactor until completely transparent. Then adjust the pH to 4.5-5.2 with citric acid or lactic acid to prevent gelation. The chitosan solution is then obtained.

[0039] (2) Preparation of hydrogen-rich water: Purified hydrogen gas with a volume concentration of 99.99% was dissolved in a high-pressure hydrogen dissolving device at room temperature at a rate of 200 mL / min. -1The hydrogen is released into 2L of distilled water at a rate that lasts for 2 hours until the dissolved hydrogen concentration is ≥1.5ppm, thus producing hydrogen-rich water.

[0040] (3) Preparation of alkyl polysaccharide pre-solution: Mix 10g octyl glucoside and 10g decyl glucoside, and add 50g of hydrogen-rich water prepared in step (2). The weight of the added hydrogen-rich water is 2.5 times that of the mixture of octyl glucoside and decyl glucoside. Stir at 200rpm for 15min in a sealed magnetic reactor to obtain alkyl polysaccharide pre-solution.

[0041] (4) Preparation of bactericide: The chitosan solution obtained in step (1) and the alkyl polysaccharide pre-solution obtained in step (3) are slowly mixed and stirred at 300 rpm for 20 min in a sealed magnetic reactor to obtain the mother liquor; under stirring, the hydrogen-rich water obtained in step (2) is slowly added to the mother liquor so that the total amount of hydrogen-rich water accounts for 71% of the total weight of the mother liquor. After stirring for 10 min, the mixture is immediately sealed and filled to obtain the hydrogen-rich water composite polysaccharide bactericide.

[0042] Example 3:

[0043] Material ratio: 9wt% chitosan, 10wt% octyl glucoside, 10wt% decyl glucoside and 71wt% hydrogen-rich water.

[0044] Preparation method of hydrogen-rich water complex polysaccharide bactericide:

[0045] (1) Preparation of chitosan solution: Slowly add 9g of chitosan to 0.135g of citric acid or lactic acid solution. The amount of citric acid or lactic acid solution is 1.5% of the total weight of chitosan. In a sealed magnetic reactor, stir at 400rpm for 30min until completely transparent. Then adjust the pH to 4.5-5.2 with citric acid or lactic acid to prevent gelation. The chitosan solution is then obtained.

[0046] (2) Preparation of hydrogen-rich water: Purified hydrogen gas with a volume concentration of 99.99% was dissolved in a high-pressure hydrogen dissolving device at room temperature at a rate of 400 mL / min. -1 The hydrogen is released into 4L of distilled water at a rate that lasts for 4 hours until the dissolved hydrogen concentration is ≥1.5ppm, thus producing hydrogen-rich water.

[0047] (3) Preparation of alkyl polysaccharide pre-solution: Mix 10g octyl glucoside and 10g decyl glucoside, and add 50g of hydrogen-rich water prepared in step (2). The weight of the added hydrogen-rich water is 2.5 times that of the mixture of octyl glucoside and decyl glucoside. Stir at 300rpm for 20min in a sealed magnetic reactor to obtain alkyl polysaccharide pre-solution.

[0048] (4) Preparation of bactericide: The chitosan solution obtained in step (1) and the alkyl polysaccharide pre-solution obtained in step (3) are slowly mixed and stirred at 400 rpm for 30 min in a sealed magnetic reactor to obtain the mother liquor; under stirring, the hydrogen-rich water obtained in step (2) is slowly added to the mother liquor so that the total amount of hydrogen-rich water accounts for 71% of the total weight of the mother liquor. After stirring for 15 min, the mixture is immediately sealed and filled to obtain the hydrogen-rich water composite polysaccharide bactericide.

[0049] Example 4:

[0050] Material ratio: 7.2wt% chitosan, 8wt% octyl glucoside, 8wt% decyl glucoside and 76.8wt% hydrogen-rich water.

[0051] Preparation method of hydrogen-rich water complex polysaccharide bactericide:

[0052] (1) Preparation of chitosan solution: Slowly add 7.2g of chitosan to 0.108g of citric acid or lactic acid solution. The amount of citric acid or lactic acid solution is 1.5% of the total weight of chitosan. In a sealed magnetic reactor, stir at 350rpm for 25min until completely transparent. Then adjust the pH to 4.5-5.2 with citric acid or lactic acid to prevent gelation. The chitosan solution is then obtained.

[0053] (2) Preparation of hydrogen-rich water: Purified hydrogen gas with a volume concentration of 99.99% was dissolved in a high-pressure hydrogen dissolving device at room temperature at a rate of 300 mL / min. -1 The hydrogen is released into 3L of distilled water at a rate that lasts for 3 hours until the dissolved hydrogen concentration is ≥1.5ppm, thus producing hydrogen-rich water.

[0054] (3) Preparation of alkyl polysaccharide pre-solution: Mix 8g of octyl glucoside and 8g of decyl glucoside, and add 40g of hydrogen-rich water prepared in step (2). The weight of the added hydrogen-rich water is 2.5 times that of the mixture of octyl glucoside and decyl glucoside. Stir at 250rpm for 18min in a sealed magnetic reactor to obtain the alkyl polysaccharide pre-solution.

[0055] (4) Preparation of bactericide: The chitosan solution obtained in step (1) and the alkyl polysaccharide pre-solution obtained in step (3) are slowly mixed and stirred at 350 rpm for 25 min in a sealed magnetic reactor to obtain the mother liquor; under stirring, the hydrogen-rich water obtained in step (2) is slowly added to the mother liquor so that the total amount of hydrogen-rich water accounts for 76.8% of the total weight of the mother liquor. After stirring for 12 min, the mixture is immediately sealed and filled to obtain the hydrogen-rich water composite polysaccharide bactericide.

[0056] Example 5:

[0057] Material ratio: 10.8wt% chitosan, 12wt% octyl glucoside, 12wt% decyl glucoside and 65.2wt% hydrogen-rich water.

[0058] Preparation method of hydrogen-rich water complex polysaccharide bactericide:

[0059] (1) Preparation of chitosan solution: Slowly add 10.8g of chitosan to 0.162g of citric acid or lactic acid solution. The amount of citric acid or lactic acid solution is 1.5% of the total weight of chitosan. In a sealed magnetic reactor, stir at 350rpm for 25min until completely transparent. Then adjust the pH to 4.5-5.2 with citric acid or lactic acid to prevent gelation. The chitosan solution is then obtained.

[0060] (2) Preparation of hydrogen-rich water: Purified hydrogen gas with a volume concentration of 99.99% was dissolved in a high-pressure hydrogen dissolving device at room temperature at a rate of 300 mL / min. -1 The hydrogen is released into 3L of distilled water at a rate that lasts for 3 hours until the dissolved hydrogen concentration is ≥1.5ppm, thus producing hydrogen-rich water.

[0061] (3) Preparation of alkyl polysaccharide pre-solution: Mix 12g octyl glucoside and 12g decyl glucoside, and add 60g of hydrogen-rich water prepared in step (2). The weight of the added hydrogen-rich water is 2.5 times that of the mixture of octyl glucoside and decyl glucoside. Stir at 250rpm for 18min in a sealed magnetic reactor to obtain alkyl polysaccharide pre-solution.

[0062] (4) Preparation of bactericide: The chitosan solution obtained in step (1) and the alkyl polysaccharide pre-solution obtained in step (3) are slowly mixed and stirred at 350 rpm for 25 min in a sealed magnetic reactor to obtain the mother liquor; under stirring, the hydrogen-rich water obtained in step (2) is slowly added to the mother liquor so that the total amount of hydrogen-rich water accounts for 65.2% of the total weight of the mother liquor. After stirring for 12 min, the mixture is immediately sealed and filled to obtain the hydrogen-rich water composite polysaccharide bactericide.

[0063] Example 6:

[0064] Material ratio: 8.1 wt% chitosan, 11 wt% octyl glucoside, 11 wt% decyl glucoside and 69.9 wt% hydrogen-rich water.

[0065] Preparation method of hydrogen-rich water complex polysaccharide bactericide:

[0066] (1) Preparation of chitosan solution: Slowly add 8.1g of chitosan to 0.1215g of citric acid or lactic acid solution. The amount of citric acid or lactic acid solution is 1.5% of the total weight of chitosan. In a sealed magnetic reactor, stir at 350rpm for 25min until completely transparent. Then adjust the pH to 4.5-5.2 with citric acid or lactic acid to prevent gelation. The chitosan solution is then obtained.

[0067] (2) Preparation of hydrogen-rich water: Purified hydrogen gas with a volume concentration of 99.99% was dissolved in a high-pressure hydrogen dissolving device at room temperature at a rate of 300 mL / min. -1 The hydrogen is released into 3L of distilled water at a rate that lasts for 3 hours until the dissolved hydrogen concentration is ≥1.5ppm, thus producing hydrogen-rich water.

[0068] (3) Preparation of alkyl polysaccharide pre-solution: Mix 11g of octyl glucoside and 11g of decyl glucoside, and add 55g of hydrogen-rich water prepared in step (2). The weight of the added hydrogen-rich water is 2.5 times that of the mixture of octyl glucoside and decyl glucoside. Stir at 250rpm for 18min in a sealed magnetic reactor to obtain the alkyl polysaccharide pre-solution.

[0069] (4) Preparation of bactericide: The chitosan solution obtained in step (1) and the alkyl polysaccharide pre-solution obtained in step (3) are slowly mixed and stirred at 350 rpm for 25 min in a sealed magnetic reactor to obtain the mother liquor; under stirring, the hydrogen-rich water obtained in step (2) is slowly added to the mother liquor so that the total amount of hydrogen-rich water accounts for 69.9% of the total weight of the mother liquor. After stirring for 12 min, the mixture is immediately sealed and filled to obtain the hydrogen-rich water composite polysaccharide bactericide.

[0070] Example 7:

[0071] Material ratio: 9.9wt% chitosan, 9wt% octyl glucoside, 11wt% decyl glucoside and 70.1wt% hydrogen-rich water.

[0072] Preparation method of hydrogen-rich water complex polysaccharide bactericide:

[0073] (1) Preparation of chitosan solution: Slowly add 9.9g of chitosan to 0.1485g of citric acid or lactic acid solution. The amount of citric acid or lactic acid solution is 1.5% of the total weight of chitosan. In a sealed magnetic reactor, stir at 350rpm for 25min until completely transparent. Then adjust the pH to 4.5-5.2 with citric acid or lactic acid to prevent gelation. The chitosan solution is then obtained.

[0074] (2) Preparation of hydrogen-rich water: Purified hydrogen gas with a volume concentration of 99.99% was dissolved in a high-pressure hydrogen dissolving device at room temperature at a rate of 300 mL / min. -1 The hydrogen is released into 3L of distilled water at a rate that lasts for 3 hours until the dissolved hydrogen concentration is ≥1.5ppm, thus producing hydrogen-rich water.

[0075] (3) Preparation of alkyl polysaccharide pre-solution: Mix 9g octyl glucoside and 11g decyl glucoside, and add 50g of hydrogen-rich water prepared in step (2). The weight of the added hydrogen-rich water is 2.5 times that of the mixture of octyl glucoside and decyl glucoside. Stir at 250rpm for 18min in a sealed magnetic reactor to obtain alkyl polysaccharide pre-solution.

[0076] (4) Preparation of bactericide: The chitosan solution obtained in step (1) and the alkyl polysaccharide pre-solution obtained in step (3) are slowly mixed and stirred at 350 rpm for 25 min in a sealed magnetic reactor to obtain the mother liquor; under stirring, the hydrogen-rich water obtained in step (2) is slowly added to the mother liquor so that the total amount of hydrogen-rich water accounts for 70.1% of the total weight of the mother liquor. After stirring for 12 min, the mixture is immediately sealed and filled to obtain the hydrogen-rich water composite polysaccharide bactericide.

[0077] To obtain the solution of this invention and verify its technical effects, the inventors conducted extensive experimental research, some of which are recorded below:

[0078] 1. Experimental Reagents and Instruments

[0079] Experimental reagents: Hydrogen-rich water (HRW), purified hydrogen gas with a volume ratio of 99.99% was added at room temperature at a rate of 300 mL / min. -1 The hydrogen was released at a rate of 3 L of distilled water over 3 h to obtain saturated HRW (Beijing Vitality Hydrogen Source Beverage Co., Ltd., Beijing). The concentration was measured to be 0.8 mmol·L⁻¹ using a portable hydrogen analyzer (ENH-100, Trustlex Co., Ltd., Led, Japan). -1 ), octyl glucoside, decyl glucoside, deionized water, sterile water, chitosan (deacetylated >90%), citric acid or lactic acid, cherry, carbendazim wettable powder, NaClO.

[0080] Experimental instruments: high-pressure spray equipment, CO2 analyzer, hardness tester, colorimeter, sugar-acidity meter, weighing balance, 4℃ refrigerator, constant temperature stirrer, magnetically sealed reaction vessel.

[0081] 2 Experimental Section

[0082] This experiment investigated the control effects of different spraying techniques of hydrogen-rich water-polysaccharide fungicide solution on cherry brown rot and the control effects of carbendazim fungicide solution on cherry brown rot.

[0083] 2.1 Preparation of hydrogen-rich water-based polysaccharide bactericide

[0084] The hydrogen-rich water-complex polysaccharide bactericide was prepared using the method described in Example 1 of this invention. The specific steps are as follows:

[0085] (1) Preparation of chitosan solution: Slowly add 9g of chitosan to 0.135g of citric acid or lactic acid solution. The amount of citric acid or lactic acid solution is 1.5% of the total weight of chitosan. In a sealed magnetic reactor, stir at 350rpm for 25min until completely transparent. Then adjust the pH to 4.5-5.2 with citric acid or lactic acid to prevent gelation. The chitosan solution is then obtained.

[0086] (2) Preparation of hydrogen-rich water: Using a high-pressure hydrogen dissolving device (≥0.5MPa), purified hydrogen gas with a volume concentration of 99.99% (v / v) is dissolved at room temperature at a rate of 300 mL / min. -1 The hydrogen is released into 3L of distilled water at a rate that lasts for 3 hours until the dissolved hydrogen concentration is ≥1.5ppm, thus producing hydrogen-rich water.

[0087] (3) Preparation of alkyl polysaccharide pre-solution: Mix 10g octyl glucoside and 10g decyl glucoside, and add 50g of hydrogen-rich water prepared in step (2). The weight of the added hydrogen-rich water is 2.5 times that of the mixture of octyl glucoside and decyl glucoside. Stir at 250rpm for 18min in a sealed magnetic reactor to obtain the alkyl polysaccharide pre-solution.

[0088] (4) Preparation of bactericide: The chitosan solution obtained in step (1) and the alkyl polysaccharide pre-solution obtained in step (3) are slowly mixed and stirred at 350 rpm for 25 min in a sealed magnetic reactor to obtain the mother liquor; under stirring, the hydrogen-rich water obtained in step (2) is slowly added to the mother liquor so that the total amount of hydrogen-rich water accounts for 71% of the total weight of the mother liquor. After stirring for 12 min, the mixture is immediately sealed and filled to obtain the hydrogen-rich water composite polysaccharide bactericide.

[0089] 2.2 Experimental Grouping

[0090] 2.2.1 Field spraying experiment A

[0091] (1) Experimental setup: A cherry orchard with an area of ​​2 mu was selected. The cherry varieties were Black Pearl, and the trees were of the same age and similar growth conditions. The orchard was treated with a hydrogen-rich water-based polysaccharide fungicide.

[0092] (2) Spraying method: Spray for the first time 7 days after cherry blossoms fall, spray for the second time 12 days later, and spray for the third time during the fruit enlargement period. Use high-pressure spraying equipment to control the droplet size to about 150μm, and spray 50L per acre.

[0093] 2.2.2 Field spraying experiment B

[0094] (1) Experimental setup: A cherry orchard with an area of ​​2 mu was selected. The cherry varieties were Black Pearl, and the trees were of the same age and similar growth conditions. The orchard was treated with a hydrogen-rich water-based polysaccharide fungicide.

[0095] (2) Spraying method: The first spraying is carried out 8 days after cherry blossoms fall, the second spraying is carried out 13 days later, and the third spraying is carried out during the fruit enlargement period. High-pressure spraying equipment is used to control the droplet size to about 200μm, and the spraying amount is 40L per acre.

[0096] 2.2.3 Field spraying test C

[0097] (1) Experimental setup: A cherry orchard with an area of ​​2 mu was selected. The cherry varieties were Black Pearl, and the trees were of the same age and similar growth conditions. The orchard was treated with a hydrogen-rich water-based polysaccharide fungicide.

[0098] (2) Spraying method: The first spraying is carried out 9 days after cherry blossoms fall, the second spraying is carried out 14 days later, and the third spraying is carried out during the fruit enlargement period. High-pressure spraying equipment is used to control the droplet size to about 250μm, and the spraying amount is 30L per acre.

[0099] 2.2.4 Field spraying test D

[0100] (1) Experimental setup: A cherry orchard with an area of ​​2 mu was selected. The cherry varieties were Black Pearl, and the trees were of the same age and similar growth conditions. The orchard was treated with a hydrogen-rich water-based polysaccharide fungicide.

[0101] (2) Spraying method: The first spraying is carried out 10 days after cherry blossoms fall, the second spraying is carried out 15 days later, and the third spraying is carried out during the fruit enlargement period. High-pressure spraying equipment is used to control the droplet size to about 300μm, and the spraying amount is 50L per acre.

[0102] 2.2.5 Carbendazim blank experimental group

[0103] (1) Preparation of carbendazim fungicide: Weigh 50g of 50% carbendazim wettable powder, add it to 50L of water, stir thoroughly until the fungicide is completely dissolved in the water and then set aside.

[0104] (2) Experimental setup: A cherry orchard with an area of ​​2 mu (approximately 0.33 hectares) was selected. The cherry varieties were Black Pearl, and the trees were of the same age and similar growth conditions. Carbendazim fungicide was used for treatment.

[0105] (3) Spraying method: Spray for the first time after the cherry blossoms fall; then spray once every 7-10 days, for a total of 3 sprays. Use a backpack manual sprayer to evenly spray the prepared carbendazim solution onto the leaves, fruits and branches of the cherry trees. When spraying, keep the nozzle 30-50cm away from the plant to ensure that the pesticide can be evenly covered to improve the control effect.

[0106] 2.3 Detection Methods

[0107] Cherry fruit indicators tested: Fruit quality indicators. Cherry fruits from each experimental group and the carbendazim blank experimental group were randomly picked, placed at room temperature for 2 hours to dissipate field heat, soaked in 1% (v / v) NaClO for 2 minutes to disinfect the fruit surface, washed with sterile water 3-5 times, air-dried naturally, and then stored in a refrigerator at 4℃. The hardness, respiration rate, weight loss rate, soluble solids content, color L* value and other indicators were measured every two days.

[0108] Methods for measuring the index: Measurement of weight loss rate.

[0109]

[0110] Hardness was measured using a hardness tester (GY-4, Adeberg, China), with 9 fruits treated per group, repeated 3 times, and the average value was taken. Respiration rate was measured using a CO2 analyzer (TEL-7001, Telaire, USA): CO2 concentration was measured on 30 fruits, and readings were taken every 20 minutes for 3 times after stabilization. The L* value of color change was measured using a colorimeter, with 9 fruits treated per group, and each fruit was treated 3 times, and the average value was taken. TSS content was measured using a sugar-acidity meter (Atago PAL-BX / ACID1, ATAGO, Japan), with 9 fruits treated per group, and each fruit treated 3 times, and the average value was taken.

[0111] 2.4 Test Results

[0112] The detection indicators are shown in Table 1:

[0113] Table 1: Results of detection indicators for each field spraying test group and the carbendazim blank test group

[0114]

[0115] Table 1 shows that during storage, the L* values ​​of cherries treated with each field spraying experimental group were all higher than those of the carbendazim control group during the same period, indicating that this hydrogen-rich water-based compound polysaccharide fungicide can effectively reduce the occurrence of fruit browning. The increases in respiration rate and weight loss rate in the four field spraying experimental groups were all smaller than those in the carbendazim control group, indicating that this hydrogen-rich water-based compound polysaccharide fungicide can effectively reduce the respiration rate and weight loss rate of the fruit, thereby reducing the occurrence of cherry fruit brown rot. The TSS and firmness values ​​of the four field spraying experimental groups were all higher than those in the carbendazim control group, indicating that this hydrogen-rich water-based compound polysaccharide fungicide can effectively maintain stable fruit quality, especially reducing the decrease in peel firmness and the decrease in TSS of the pulp caused by peel dehydration.

[0116] Experiments show that the hydrogen-rich water-based polysaccharide bactericide and its application method of the present invention have significant effects in preventing and controlling cherry brown rot and improving fruit quality.

Claims

1. A hydrogen-rich water-based compound polysaccharide fungicide for controlling cherry brown rot, characterized in that: The hydrogen-rich water composite polysaccharide bactericide is prepared from chitosan, octyl glucoside, decyl glucoside, and hydrogen-rich water; the chitosan accounts for 7.2-10.8 wt% of the total weight of the hydrogen-rich water composite polysaccharide bactericide, the octyl glucoside accounts for 8-12 wt% of the total weight of the hydrogen-rich water composite polysaccharide bactericide, the decyl glucoside accounts for 8-12 wt% of the total weight of the hydrogen-rich water composite polysaccharide bactericide, and the hydrogen-rich water accounts for 76.8-65.2 wt% of the total weight of the hydrogen-rich water composite polysaccharide bactericide.

2. The hydrogen-rich water-based compound polysaccharide fungicide for controlling cherry brown rot according to claim 1, characterized in that: The chitosan accounts for 8.1-9.9 wt% of the total weight of the hydrogen-rich water composite polysaccharide bactericide, the octyl glucoside accounts for 9-11 wt% of the total weight of the hydrogen-rich water composite polysaccharide bactericide, the decyl glucoside accounts for 9-11 wt% of the total weight of the hydrogen-rich water composite polysaccharide bactericide, and the hydrogen-rich water accounts for 73.9-68.1 wt% of the total weight of the hydrogen-rich water composite polysaccharide bactericide.

3. The hydrogen-rich water-based compound polysaccharide fungicide for controlling cherry brown rot according to claim 1 or 2, characterized in that: The chitosan accounts for 9 wt% of the total weight of the hydrogen-rich water composite polysaccharide bactericide, the octyl glucoside accounts for 10 wt% of the total weight of the hydrogen-rich water composite polysaccharide bactericide, the decyl glucoside accounts for 10 wt% of the total weight of the hydrogen-rich water composite polysaccharide bactericide, and the hydrogen-rich water accounts for 71 wt% of the total weight of the hydrogen-rich water composite polysaccharide bactericide.

4. The method for preparing the hydrogen-rich water-based composite polysaccharide fungicide for controlling cherry brown rot according to any one of claims 1-3, characterized in that: The preparation method of the hydrogen-rich water composite polysaccharide bactericide is carried out according to the following steps: (1) Preparation of chitosan solution: Slowly add chitosan to citric acid or lactic acid solution. The amount of citric acid or lactic acid solution is 1.5% of the total weight of chitosan. In a sealed magnetic reactor, stir at 300-400 rpm for 20-30 minutes until completely transparent. Then adjust the pH to 4.5-5.2 with citric acid or lactic acid to prevent gelation. The chitosan solution is then obtained. (2) Preparation of hydrogen-rich water: Using a high-pressure hydrogen dissolving device, purified hydrogen gas with a volume concentration of 99.99% is dissolved at room temperature at a rate of 200-400 mL / min. -1 The hydrogen is released at a rate of 2-4L into 2-4h for 2-4h until the dissolved hydrogen concentration is ≥1.5ppm, thus producing hydrogen-rich water. (3) Preparation of alkyl polysaccharide pre-solution: After mixing octyl glucoside and decyl glucoside, add hydrogen-rich water obtained in step (2). The weight of the added hydrogen-rich water is 2.5 times that of the mixture of octyl glucoside and decyl glucoside. Stir at 200-300 rpm for 15-20 min in a sealed magnetic reactor to obtain alkyl polysaccharide pre-solution. (4) Preparation of bactericide: The chitosan solution obtained in step (1) and the alkyl polysaccharide pre-solution obtained in step (3) are slowly mixed and stirred at 300-400 rpm for 20-30 min in a sealed magnetic reactor to obtain the mother liquor; under stirring, the hydrogen-rich water obtained in step (2) is slowly added to the mother liquor so that the total amount of hydrogen-rich water accounts for 76.8-65.2% of the total weight of the mother liquor. After stirring for 10-15 min, the mixture is immediately sealed and filled to obtain the hydrogen-rich water composite polysaccharide bactericide.

5. The preparation method according to claim 4, characterized in that: In step (1), the chitosan solution is prepared by slowly adding chitosan to a citric acid or lactic acid solution, the amount of which is 1.5% of the total weight of the chitosan. In a sealed magnetic reactor, the mixture is stirred at 350 rpm for 25 minutes until it is completely transparent. Then, the pH is adjusted to 4.5-5.2 with citric acid or lactic acid to prevent gelation, thus obtaining the chitosan solution.

6. The preparation method according to claim 4, characterized in that: In step (2), hydrogen-rich water is prepared by using a high-pressure hydrogen dissolving device to dissolve purified hydrogen gas with a volume concentration of 99.99% at room temperature at a rate of 300 mL / min. -1 The hydrogen is released into 3L of distilled water at a rate that lasts for 3 hours until the dissolved hydrogen concentration is ≥1.5ppm, thus producing hydrogen-rich water.

7. The preparation method according to claim 4, characterized in that: In step (3), an alkyl polysaccharide pre-solution is prepared by mixing octyl glucoside and decyl glucoside and adding hydrogen-rich water obtained in step (2). The weight of the added hydrogen-rich water is 2.5 times that of the mixture of octyl glucoside and decyl glucoside. The mixture is stirred at 250 rpm for 18 minutes in a sealed magnetic reactor to obtain the alkyl polysaccharide pre-solution.

8. The preparation method according to claim 4, characterized in that: In step (4), the bactericide is prepared by slowly mixing the chitosan solution obtained in step (1) and the alkyl polysaccharide pre-solution obtained in step (3) in a sealed magnetic reactor and stirring at 350 rpm for 25 min to obtain the mother liquor. While stirring, the hydrogen-rich water obtained in step (2) is slowly added to the mother liquor so that the total amount of hydrogen-rich water accounts for 71% of the total weight of the mother liquor. After stirring for 12 min, the mixture is immediately sealed and filled to obtain the hydrogen-rich water composite polysaccharide bactericide.

9. The application of the hydrogen-rich water-based polysaccharide bactericide according to any one of claims 1-8 in the treatment and prevention of cherry brown rot, characterized in that: The application method of the hydrogen-rich water compound polysaccharide bactericide is as follows: dilute the high-concentration hydrogen-rich water compound polysaccharide bactericide 1000-1500 times, and spray it for the first time 7-10 days after cherry blossoms fall. Spray it for the second time after an interval of 10-15 days, and spray it for the third time during the fruit enlargement period. Use a high-pressure spraying device to spray the bactericide evenly on the leaves, fruits and branches of the cherry trees. Control the spray droplet size to 100-300μm, and the spraying amount is 30-50L per acre.

10. The application according to claim 9, characterized in that: The application method of the hydrogen-rich water compound polysaccharide bactericide is as follows: dilute the high-concentration hydrogen-rich water compound polysaccharide bactericide 1000 times, and spray it for the first time 10 days after cherry blossoms fall, spray it for the second time after an interval of 15 days, and spray it for the third time during the fruit enlargement period; use high-pressure spraying equipment to spray the bactericide evenly on the leaves, fruits and branches of cherry trees, control the spray droplet size to 300μm, and spray 50L per acre.

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