Atractylodes macrocephala koidz successive cropping obstacle retarding agent
By using a combination of compound microbial agents and organic fertilizers, including actinomycetes and Bacillus laterosporus, the disease problems caused by continuous cropping of Atractylodes macrocephala were solved, the yield and quality of Atractylodes macrocephala were improved, the soil environment was improved, and the obstacles of continuous cropping were mitigated.
Patent Information
- Application Number
- CN202511129454.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-08-13
- Publication Date
- 2025-12-02
AI Technical Summary
Continuous cropping of Atractylodes macrocephala leads to root rot, white mold disease, and damping-off, resulting in decreased yield and quality and causing economic losses to farmers.
By using a combination of compound microbial agents and organic fertilizers, including actinomycetes and Bacillus laterosporus, the continuous cropping obstacles of Atractylodes macrocephala can be mitigated by inhibiting pathogenic fungi and improving the soil microbial community.
It effectively improves the quality and yield of Atractylodes macrocephala, alleviates continuous cropping obstacles, improves the soil environment, and inhibits soil-borne pathogens.
Smart Images
Figure SMS_1
Abstract
Description
Technical Field
[0001] This invention relates to the field of biological agent preparation technology, specifically to a remedial agent for continuous cropping obstacles of Atractylodes macrocephala. Background Technology
[0002] Atractylodes macrocephala is a plant of the Asteraceae family, mainly distributed in East my country, Central China, and North China. It has properties such as sweetness, bitterness, and warmth, and is believed to have spleen-strengthening, stomach-benefiting, dampness-drying, and diuretic effects, and is known as the "King of Atractylodes".
[0003] Atractylodes macrocephala is susceptible to continuous cropping and should not be planted in the same plot of land. Even under normal management conditions, continuous cropping of Atractylodes macrocephala can easily lead to cropping obstacles, such as root rot, white mold, and damping-off, resulting in a decline in yield and quality and causing significant economic losses to farmers. Summary of the Invention
[0004] To address the shortcomings of existing technologies, this invention provides a remedial agent for continuous cropping obstacles of Atractylodes macrocephala, which solves the technical problem of reduced yield and quality caused by continuous cropping of Atractylodes macrocephala.
[0005] To achieve the above objectives, the present invention provides the following technical solution:
[0006] This invention provides a remedial agent for continuous cropping obstacles of Atractylodes macrocephala, comprising the following components in parts by weight:
[0007] Compound microbial agent 14.5-30;
[0008] Organic fertilizer 2000-3000.
[0009] Preferably, the compound microbial agent includes actinomycetes and Bacillus laterosporus.
[0010] Preferably, the mass ratio of the actinomycetes to Bacillus laterosporus is 10-20:4.5-8.0.
[0011] Preferably, the mass ratio of the actinomycetes to Bacillus laterosporus is 10-15:4.5-6.0.
[0012] Preferably, the mass ratio of the actinomycetes to Bacillus laterosporus is 10:4.5.
[0013] Preferably, the organic fertilizer includes miscellaneous organic fertilizer and compost.
[0014] Preferably, the miscellaneous organic fertilizer includes any one of humic acid fertilizer, oilseed meal fertilizer, and soil fertilizer.
[0015] Preferably, the compost includes compost or biogas digester fertilizer.
[0016] Compared with existing technologies, it has the following beneficial effects:
[0017] This application's Atractylodes macrocephala continuous cropping obstacle mitigation agent includes a compound microbial agent and organic fertilizer. The compound microbial agent includes actinomycetes and Bacillus laterosporus. Actinomycetes can produce antibiotics to inhibit pathogenic fungi and improve the soil microbial community; Bacillus laterosporus can produce antibacterial substances to inhibit pathogens and promote plant growth. The combination of the two can inhibit soil-borne pathogens through different mechanisms of action, improve the soil environment, and thus effectively mitigate the continuous cropping obstacle of Atractylodes macrocephala, and improve the quality and yield of continuously cropped Atractylodes macrocephala. Detailed Implementation
[0018] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention are described clearly and completely. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.
[0019] To better understand the above technical solution, the following will provide a detailed explanation of the above technical solution in conjunction with specific implementation methods.
[0020] I. Preparation Method
[0021] Example 1
[0022] This embodiment provides a remedial agent for continuous cropping obstacles of Atractylodes macrocephala, comprising the following components: Actinomycetes and Bacillus laterosporus, wherein the mass ratio of Actinomycetes to Bacillus laterosporus is 10:4.5.
[0023] A *Atractylodes macrocephala* planting base located in Lixiaomiao Village, Feihe Town, Qiaocheng District, Bozhou City (115°33'~116°06' E, 33°25'~34°05' N) was selected as the experimental site for planting *Atractylodes macrocephala* using a continuous cropping obstacle mitigation agent. The experimental site had been planted with *Atractylodes macrocephala* for one year. The area has a temperate monsoon climate, characterized by mild temperatures, ample sunshine, moderate rainfall, and distinct seasons. The average annual precipitation is 867.2 mm, the average annual temperature is 15.2℃, the average annual sunshine duration is 2040.9 h, and the average annual frost-free period is 219 days. The experimental plot area was 30 m². 2 (10 m × 3 m). Row spacing is set at 30 cm × 35 cm, with a density of 5868 plants / 667 m². 2 The experimental plot was planted in 8 rows, each row was 10m long, with 33 plants in each row.
[0024] Atractylodes macrocephala was planted using a cropping obstacle mitigation agent. Organic fertilizer was applied as base fertilizer at the time of transplanting. Actinomycetes, Bacillus laterosporus, and organic fertilizer were mixed and applied at the following rates: Actinomycetes 10 kg / hm². 2 4.5 kg / hm of Bacillus laterosporus 2 3000 kg / hm² of organic fertilizer 2 .
[0025] The organic fertilizer was purchased from Shandong Yuanquan Fertilizer Co., Ltd. The organic fertilizer has a total nutrient content (N+P2O5+K2O) of ≥5.0% and an organic matter content of ≥45%. The Atractylodes macrocephala seedlings were sourced from Bozhou Ruihang Chinese Medicinal Herb Seedling Sales Co., Ltd., and the Bacillus laterosporus and Actinomycetes were purchased from Henan Wobao Biotechnology Co., Ltd.
[0026] Example 2
[0027] The difference between this embodiment and Embodiment 1 is that a replanting obstacle mitigation agent for Atractylodes macrocephala is used for planting Atractylodes macrocephala, organic fertilizer is applied as base fertilizer at the time of transplanting, and actinomycetes, Bacillus laterosporus, and organic fertilizer are mixed and applied at the following rates: actinomycetes 11 kg / hm². 2 5.5 kg / hm of Bacillus laterosporus 2 3000 kg / hm² of organic fertilizer 2 Everything else is the same as in Example 1.
[0028] Example 3
[0029] The difference between this embodiment and Embodiment 1 is that a replanting obstacle mitigation agent for Atractylodes macrocephala is used for planting Atractylodes macrocephala, organic fertilizer is applied as base fertilizer at the time of transplanting, and actinomycetes, Bacillus laterosporus, and organic fertilizer are mixed and applied at the following rates: actinomycetes 12 kg / hm². 2 6.0 kg / hm of Bacillus laterosporus 2 3000 kg / hm² of organic fertilizer 2 Everything else is the same as in Example 1.
[0030] Comparative Example 1
[0031] The difference between this comparative example and Example 1 is that the Atractylodes macrocephala continuous cropping obstacle mitigator only includes actinomycetes, and the dosage of actinomycetes is 14.5 kg / hm. 2 3000 kg / hm² of organic fertilizer 2 Everything else is the same as in Example 1.
[0032] Comparative Example 2
[0033] The difference between this comparative example and Example 1 is that the Atractylodes macrocephala continuous cropping obstacle mitigator only includes Bacillus laterosporus, with a dosage of 14.5 kg / hm². 2 3000 kg / hm² of organic fertilizer 2 The rest is the same as in Example 1.
[0034] II. Testing Methods
[0035] 1. The survival rate, disease incidence rate, and mortality rate of Atractylodes macrocephala planted in the experimental fields of Examples 1-3 and Comparative Examples 1-2 were statistically analyzed and calculated. The specific methods are as follows:
[0036] Survival rate: The number of surviving plants was recorded at the time of harvest of Atractylodes macrocephala. Survival rate = (number of surviving plants / total number of plants) × 100%;
[0037] Disease susceptibility rate: For each phenological stage of Atractylodes macrocephala, the number of infected plants was recorded. Disease susceptibility rate = number of infected plants / total number of plants × 100%;
[0038] Mortality rate: Record the number of dead plants for each phenological stage of Atractylodes macrocephala. Mortality rate = number of dead plants / total number of plants × 100%.
[0039] 2. The yield of Atractylodes macrocephala in Examples 1-3 and Comparative Examples 1-2 was determined.
[0040] After the Atractylodes macrocephala seedlings withered, the tubers were dug up and weighed according to the area.
[0041] 3. Determination of quality indicators of Atractylodes macrocephala from the experimental sites of Examples 1-3 and Comparative Examples 1-2
[0042] The moisture content of Atractylodes macrocephala rhizomes was determined according to the second method in General Chapter 0832 of the 2020 edition of the Chinese Pharmacopoeia, Part IV; the total ash content of Atractylodes macrocephala rhizomes was determined according to General Chapter 2302; and the extract of Atractylodes macrocephala rhizomes was determined by the hot extraction method under the alcohol-soluble extract determination method in General Chapter 2201.
[0043] 4. Determination of Atractylodes macrocephala polysaccharides in Examples 1-3 and Comparative Examples 1-2
[0044] (1) Preparation of reference solution: Take 9.10 mg of anhydrous glucose reference standard dried to constant weight at 105℃, accurately weigh it, put it in a 25 mL volumetric flask, add water to dissolve and dilute to the mark, shake well, and you will get (1 mL contains 0.36 mg of anhydrous glucose).
[0045] (2) Preparation of the standard curve: Accurately measure 0.10 mL, 0.20 mL, 0.40 mL, 0.60 mL, 0.80 mL, 1.00 mL, and 1.20 mL of the reference solution and place them in 10 mL stoppered test tubes respectively. Add 2.00 mL of water, shake well, add 0.2% anthrone-sulfuric acid solution to the mark in an ice-water bath, mix well, cool, and then place in a warm water bath for 10 min. Remove and immediately place in an ice-water bath for 10 min. Remove and use the corresponding reagent as a blank, measure the absorbance at a wavelength of 582 nm. Plot absorbance as the ordinate and concentration as the abscissa to obtain the regression equation Y = 1.4286X + 0.0617(R² + π / 4)². 2 =0.9949).
[0046] (3) Sample preparation: Accurately weigh 0.25 g of Atractylodes macrocephala sample, place it in a 250 mL Erlenmeyer flask, add 150 mL of 80% ethanol, heat in a water bath and reflux for 1 h, filter, keep the filter residue, add 150 mL of water, heat in a water bath and reflux for 1 h, cool, filter, take 1 mL of filtrate, and according to the method in section ②, starting from "add water and place 2.00 mL", determine the absorbance according to the law, and calculate the polysaccharide content through the regression equation.
[0047] III. Test Results
[0048] The quality and yield test results of Atractylodes macrocephala in Examples 1-3 and Comparative Examples 1-2 are shown in Table 1.
[0049] Table 1. Quality and yield of Atractylodes macrocephala in Examples 1-3 and Comparative Examples 1-2.
[0050]
[0051] As shown in Table 1, the quality and yield of Atractylodes macrocephala in the experimental plots of Examples 1-3 were superior to those in Comparative Examples 1 and 2. This demonstrates that the Atractylodes macrocephala continuous cropping obstacle mitigator can effectively alleviate continuous cropping obstacles. This is because the Atractylodes macrocephala continuous cropping obstacle mitigator of this application includes actinomycetes and Bacillus laterosporus. Actinomycetes can produce antibiotics to inhibit pathogenic fungi and improve the soil microbial flora; Bacillus laterosporus can produce antibacterial substances to inhibit pathogens and promote plant growth. The combination of these two can inhibit soil-borne pathogens through different pathways of action, improve the soil environment, and thus effectively alleviate continuous cropping obstacles of Atractylodes macrocephala, improving the quality and yield of continuously cropped Atractylodes macrocephala.
[0052] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitations, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes said element.
[0053] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit it. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.
[0054] The present invention has been illustrated with the above embodiments to describe the detailed process flow of the present invention. However, the present invention is not limited to the above detailed process flow, that is, it does not mean that the present invention must rely on the above detailed process flow to be implemented. Those skilled in the art should understand that any improvements to the present invention, equivalent substitutions of the raw materials of the product of the present invention, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.
Claims
1. A remedial agent for continuous cropping obstacles of Atractylodes macrocephala, characterized in that, The components include the following parts by weight: Compound microbial agent 14.5-30; Organic fertilizer 2000-3000.
2. The Atractylodes macrocephala continuous cropping obstacle mitigation agent as described in claim 1, characterized in that, The compound microbial agent includes actinomycetes and Bacillus laterosporus.
3. The Atractylodes macrocephala continuous cropping obstacle mitigation agent as described in claim 1, characterized in that, The mass ratio of the actinomycetes to Bacillus laterosporus is 10-20:4.5-8.
0.
4. The Atractylodes macrocephala continuous cropping obstacle mitigation agent as described in claim 1, characterized in that, The mass ratio of the actinomycetes to Bacillus laterosporus is 10-15:4.5-6.
0.
5. The Atractylodes macrocephala continuous cropping obstacle mitigation agent as described in claim 1, characterized in that, The mass ratio of the actinomycetes to Bacillus laterosporus is 10:4.
5.
6. The Atractylodes macrocephala continuous cropping obstacle mitigation agent as described in claim 1, characterized in that, The organic fertilizer includes miscellaneous organic fertilizers and compost.
7. The Atractylodes macrocephala continuous cropping obstacle mitigation agent as described in claim 6, characterized in that, The miscellaneous organic fertilizers include any one of humic acid fertilizers, oilseed meal fertilizers, and soil fertilizers.
8. The Atractylodes macrocephala continuous cropping obstacle mitigation agent as described in claim 6, characterized in that, The compost includes compost or biogas digester fertilizer.