Plant extraction combined preparation for preventing and treating citrus diseases

Abstract

The invention provides a plant extraction combined preparation for preventing and treating citrus diseases, and belongs to the technical field of bactericides. The composition comprises the following components: monoterpene alcohol essential oil, monoterpene resin, a natural surfactant and fermented cell sap. The preparation provided by the invention is composed of natural components, and organisms and products thereof obtained by any genetic engineering are not used; chemical synthetic pesticides, chemical fertilizers, growth regulators, feed additives and the like; the related requirements of organic planting are met.

Description

Technical Field

[0001] This invention belongs to the field of fungicide technology, and particularly relates to a plant extract combination preparation for the prevention and control of citrus diseases. Background Technology

[0002] Organic citrus refers to a new production method that follows ecological principles and natural laws in agricultural production, adheres to the principle of dynamic interaction between soil, plants, animals, microorganisms, humans, ecosystems and environment, coordinates the balance between citrus planting and breeding, adopts new technologies for sustainable development, and maintains a continuous and stable production.

[0003] Anthracnose is a prevalent and significant disease in citrus-producing areas of my country, affecting numerous varieties and types of citrus fruits, including mandarins, oranges, pomelos, lemons, citrons, and Buddha's hand citrons. It causes rot, drying, and drop of leaves, flowers, twigs, and fruits, severely impacting tree vigor and yield. Acute anthracnose in citrus is particularly problematic. Because the pathogen invades the citrus tissues during the young leaf and fruit stages, under adverse environmental conditions such as natural disasters, or when the tree's resistance is weak due to poor management, the latent anthracnose pathogen can multiply and spread rapidly, causing widespread disease outbreaks and even orchard destruction in a short period, resulting in substantial losses for fruit growers and the local industry.

[0004] Citrus bacterial canker disease is mainly caused by Xanthomonas citri subsp. Citri, Xcc. It primarily infects plants in the Rutaceae family, such as those in the Citrus and Citrus genera. Citrus fruits and those containing citrus genes are more susceptible to the disease. The canker disease can infect the leaves, fruits, and branches of citrus fruits, forming characteristic crater-like lesions. The disease spreads rapidly and is difficult to prevent and treat. Severe damage to leaves and branches can lead to leaf drop and twig dieback. Infected fruits may develop lesions, reducing their economic value, or even crack and fall off in severe cases. Improper control often results in significant economic losses.

[0005] Citrus Huanglongbing (HLB) is a devastating disease caused by the Gram-negative bacterium Candidatus Liberibacterasiaticus. It primarily infects the phloem sieve tubes of citrus plants, disrupting the plant's nutrient transport system and leading to tree decline, fruit deformities, and even death. This disease is classified as a Class A crop disease.

[0006] Camellia seed cake (also known as tea cake, tea bran, or tea seed cake) is the residue left after pressing camellia seeds for oil; it is an agricultural by-product. Tobacco stalks refer to the remaining stems of tobacco plants after the tobacco leaves are harvested; they are classified as straw resources within agricultural waste.

[0007] Both camellia oil cake and tobacco stalks possess certain natural bactericidal properties, but their mechanisms of action and application methods differ. The bactericidal activity of camellia oil cake (tea cake) primarily derives from tea saponins, polyphenols, and flavonoids. Tea saponins, in particular, achieve broad-spectrum antibacterial activity by disrupting microbial cell membranes, effectively targeting bacteria, fungi, and parasites. It is commonly used for agricultural disease control, aquatic pond disinfection, and the development of daily chemical products. Its advantage lies in its direct application and lack of residue after degradation. In contrast, the bactericidal effect of tobacco stalks relies on nicotine, tannins, and phenolic substances. Nicotine has a strong inhibitory effect on bacteria, fungi, and nematodes. However, the raw product contains toxicity and requires detoxification through composting or processing into bio-organic fertilizer before use. It is suitable for soil disease control or the manufacture of environmentally friendly materials. Both represent the resource utilization of agricultural waste. Camellia oil cake leans towards direct antibacterial application, while tobacco stalks require secondary processing to balance safety and functionality. Both, however, demonstrate the potential of natural components to replace chemicals in green agriculture.

[0008] However, due to the requirements of organic farming, chemical fertilizers and pesticides cannot be used; therefore, how to treat and prevent citrus anthracnose, canker, Huanglongbing or other diseases in an organic farming environment is currently a major challenge. Summary of the Invention

[0009] The purpose of this invention is to provide a broad-spectrum formulation capable of controlling most pathogenic bacteria in citrus fruits under organic farming conditions. To achieve this objective, this invention provides a plant extract formulation comprising the following components, in parts by weight: 1-3 parts monoterpene alcohol essential oil 1-3 parts of monoterpene resin 0.2-0.4 parts of natural surfactant 10 portions of fermented cell broth The fermented cell broth is a product obtained by microbial fermentation of at least one of garlic cell broth, onion cell broth, and mustard cell broth. The microorganisms include at least one of the following: At least one of Lactobacillus rhamnosus, Bacillus amyloliquefaciens, and Bacillus subtilis.

[0010] Preferably, the monoterpene alcohol essential oil includes at least one of the following components: Linalool oil, geranium oil, and eucalyptus oil.

[0011] Preferably, the monoterpene resin comprises at least one of the following components: Liquidambar resin, rosin, and Cuban resin.

[0012] Preferably, the natural surfactant includes at least one of the following components: Tea saponins, saponins, rhamnolipids, and sophorolipids. Preferably, it also includes lecithin; lecithin is used to encapsulate monoterpene essential oils and monoterpene resins.

[0013] Preferably, the method for preparing fermented cell slurry is as follows: after crushing the plant raw material, filter it and take the filtrate as the sole nutrient source, and ferment it in a liquid fermentation manner for 18-36 hours at a temperature of 25-40℃.

[0014] The present invention also provides the application of the aforementioned plant extract combination preparation in the prevention and control of citrus diseases.

[0015] Preferably, the citrus disease is at least one of citrus Huanglongbing, citrus canker, and citrus anthracnose.

[0016] Preferably, the medicine is applied by spraying or injection.

[0017] According to the definition of organic production in GB / T 19630-2019 Requirements for Organic Product Production, Processing, Labelling and Management Systems: Organic production is an agricultural production method that follows specific production principles, does not use genetically engineered organisms or their products, and does not use chemically synthesized pesticides, fertilizers, growth regulators, feed additives, or other substances. It follows natural laws and ecological principles, coordinates the balance between crop farming and animal husbandry, and maintains a sustainable and stable production system.

[0018] Therefore, organic farming methods severely limit the raw materials that can be used, resulting in a very limited range of mature technological solutions available. This invention, however, uses plant-derived components as its core, combined with microbial fermentation and natural encapsulation technology, aligning with the principles of organic farming that prohibit the use of chemically synthesized pesticides and emphasize ecological cycles and biological control.

[0019] The main active ingredient consists of three parts: Monoterpene essential oils: These are natural essential oils containing (total content greater than 50%) large amounts of components such as linalool, geraniol, and eucalyptol. The lipophilic nature of monoterpene alcohols allows them to embed into bacterial cell membranes, disrupting membrane structure and causing leakage of cell contents. They can also interfere with key bacterial metabolic enzymes such as DNA polymerase and RNA polymerase, blocking protein and nucleic acid synthesis.

[0020] Monoterpene resins refer to natural resins containing large amounts (total content greater than 50%) of components such as limonene, terpinene, caryophyllene, and β-pinene, similar to monoterpene alcohols. Monoterpenes penetrate bacterial cell membranes through hydrophobic interactions, leading to changes in membrane fluidity and ion imbalance; they also inhibit enzymes related to bacterial energy metabolism. However, due to the different target sites of monoterpene alcohols and monoterpenes, their combined use can achieve synergistic effects.

[0021] Fermented cell broth: Garlic contains allicin, onions contain allicin analogues (thiosulfinates), thiols and quercetin; mustard greens contain glucosinolates, etc.; these components themselves have certain bactericidal effects; this invention utilizes microbial fermentation to form more secondary metabolites to further increase the content of its antibacterial substances.

[0022] To enhance its effectiveness, the present invention also contains the following excipients: Natural surfactants such as tea saponins and rhamnolipids have both emulsifying and penetrating functions, which can improve the adhesion of the drug solution to the waxy surface of citrus fruits and enhance the efficacy of the drug by destroying the biofilm of pathogens.

[0023] Lecithin: By encapsulating essential oils and resinous components, lecithin can delay volatilization, improve stability, and reduce the irritation of high concentrations to citrus plants.

[0024] The beneficial technical effects of this invention are reflected in the fact that all the preparations are composed of natural ingredients, without the use of any genetically engineered organisms or their products; chemically synthesized pesticides, fertilizers, growth regulators, feed additives, etc.; and they meet the relevant requirements of organic farming. Detailed Implementation

[0025] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0026] In the description of this invention, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of the stated features. In the description of this invention, "a plurality of" means two or more, unless otherwise explicitly specified.

[0027] In the description of this invention, the term "for example" is used to mean "used as an example, illustration, or description." Any embodiment described as "for example" in this invention is not necessarily to be construed as being more preferred or advantageous than other embodiments. The following description is provided to enable any person skilled in the art to make and use the invention. Details are set forth in the following description for purposes of explanation. It should be understood that those skilled in the art will recognize that the invention can be made without using these specific details. In other instances, well-known structures and processes will not be described in detail to avoid obscuring the description of the invention with unnecessary detail. Therefore, the invention is not intended to be limited to the embodiments shown, but is consistent with the broadest scope of the principles and features disclosed herein.

[0028] Unless otherwise specified, the experimental methods used in the specific implementation methods are all conventional methods, and the materials and reagents used are all commercially available unless otherwise specified.

[0029] In this invention, unless otherwise specified, "%" represents a percentage by mass; the raw materials and reagents used are all commercially available products.

[0030] The water used in this invention is preferably pure water (RO water).

[0031] The linalool used in this invention is specifically camphor tree (Cinnamomum camphora) leaf oil. The geranium oil used in this invention is specifically geranium (PELARGONIUM GRAVEOLENS) leaf oil. The blue gum eucalyptus oil used in this invention is specifically blue gum eucalyptus (Eucalyptus globulus) leaf oil.

[0032] The spruce resin used in this invention is specifically the dried resin of the Liquidambar formosana Hance tree.

[0033] The rosin used in this invention is specifically the resin of Pinus sylvestris.

[0034] The Cuban resin used in this invention is specifically the resin of the Cuban balsam tree (COPAIFERA OFFICINALIS).

[0035] The lecithin used in this invention is soybean lecithin.

[0036] The tea tree essential oil used in this invention is the leaf oil of Melaleuca alternifolia (MELALEUCA ALTERNIFOLIA).

[0037] The cinnamon essential oil used in this invention is cinnamon (CINNAMOMUM CASSIA) oil.

[0038] The frankincense resin used in this invention is BOSWELLIA SERRATA gum.

[0039] Example 1 A plant extract formulation, comprising the following components in parts by weight: 1 part camphor oil 1 part rosin 0.2 parts tea saponin 10 portions of fermented garlic cell broth; The preparation method of fermented garlic cell slurry is as follows: take fresh garlic, crush it and filter it to obtain the filtrate, add 0.5wt% Bacillus subtilis to the garlic filtrate; start with pH 5.5 and start with temperature 32℃ for 36h; control the process temperature between 32-40℃.

[0040] Mix linalool oil, rosin, tea saponin, and fermented garlic cell liquid, then seal and store for later use.

[0041] Example 2 A plant extract formulation, comprising the following components in parts by weight: 2 parts geranium oil 2 parts of maple resin Saponins 0.3 parts 10 portions of fermented onion cell broth; The preparation method of fermented onion cell slurry is as follows: Take fresh onion, crush it and filter it to obtain the filtrate, add 0.5wt% Lactobacillus rhamnosus to the onion filtrate; start with pH 6.0, start with temperature 30℃ and culture for 18h, and control the process temperature between 32-40℃.

[0042] Mix geranium oil, styrax resin, saponins, and fermented onion cell sap, then seal and store for later use.

[0043] Example 3 A plant extract formulation, comprising the following components in parts by weight: 3 parts blue gum eucalyptus oil 3 parts Cuban resin 0.4 parts rhamnolipid 10 portions of fermented onion cell broth; The preparation method of fermented mustard cell broth is as follows: take fresh mustard, crush it and filter it to obtain the filtrate, add 0.5wt% Bacillus amyloliquefaciens to the mustard filtrate; start with pH 5.5, start with temperature 24℃ and culture for 30h, and control the process temperature between 28-37℃.

[0044] Mix eucalyptus oil, Cuban resin, rhamnolipin, and fermented onion cell slurry, then seal and store for later use.

[0045] Example 4 A plant extract formulation, comprising the following components in parts by weight: 1 part camphor oil 1 part rosin 0.6 parts soybean lecithin Sophorolipid 0.2 parts 10 portions of fermented garlic cell broth; The preparation method of fermented garlic cell slurry is as follows: take fresh garlic, crush it and filter it to obtain the filtrate, add 0.5wt% Bacillus subtilis to the garlic filtrate; start with pH 5.5 and start with temperature 32℃ for 36h; control the process temperature between 32-40℃.

[0046] Soybean lecithin was dissolved in chloroform to form a lecithin solution. Ethanol containing linalool oil and rosin was poured into the lecithin solution. The mixture was refluxed and stirred for 1.5 hours. The solution was removed by rotary evaporation and then freeze-dried to obtain the coated essential oil.

[0047] Mix the coated essential oil, tea saponin, and fermented garlic cell liquid, then seal and store for later use.

[0048] Example 5 A plant extract combination preparation differs from Example 1 in that: Replace camphor oil with an equal amount of tea tree oil.

[0049] Example 6 A plant extract combination preparation differs from Example 1 in that: Replace camphor oil with an equal amount of cinnamon essential oil.

[0050] Example 7 A plant extract combination preparation differs from Example 1 in that: Replace rosin with an equal amount of frankincense resin.

[0051] Example 8 A plant extract combination preparation differs from Example 1 in that: The linalool oil has a weight of 2 parts and no rosin is added.

[0052] Example 9 A plant extract combination preparation differs from Example 1 in that: The rosin content is 2 parts by weight, and no linalool is added.

[0053] Example 10 A plant extract combination preparation differs from Example 1 in that: Garlic cell sap is used instead of fermented garlic cell sap. The preparation method of garlic cell sap is as follows: take fresh garlic, crush it, filter it and obtain the filtrate.

[0054] Example 11 A plant extract combination preparation differs from Example 1 in that: Use purified water instead of fermented garlic cell broth.

[0055] The results are shown in Table 1 below.

[0056] Experiment on the control of citrus anthracnose: Field application trials were conducted to test the efficacy. The plant extract combination formulations provided in Examples 1 to 11 of this invention were tested using the methods specified in the "Guidelines for Field Efficacy Trials of Pesticides." The concentration was diluted 100 times. Each group sprayed the entire tree of 20 Newhall navel orange trees already infected with Huanglongbing (HLB) once daily for 4 consecutive days, followed by a 5-day interval, then another 4 consecutive days of spraying, repeated 5 times. Fourteen days after the final spray, all leaves of the current year's new shoots were surveyed, and the number of diseased leaves at each level was counted. Diseased leaf grading standards: Grade 0, no lesions; Grade 1, 1-5 lesions per leaf; Grade 3, 6-10 lesions per leaf; Grade 5, 11-15 lesions per leaf; Grade 7, 16-20 lesions per leaf; Grade 9, more than 21 lesions per leaf.

[0057] Then calculate the prevention and control effect based on the results; Prevention and control effect (%) = Among them, the disease index = Experiment on the prevention and control of citrus canker Similarly, using the same method as the citrus anthracnose control experiment, each set of examples was conducted on 20 Newhall navel orange trees that had already developed citrus canker.

[0058] Huanglongbing control experiment Using the same method as the citrus anthracnose control experiment, each set of examples was conducted on 20 Newhall navel orange trees that had already developed Huanglongbing (HLB).

[0059] Table 1 As shown in Table 1, the contents of essential oil components and surfactant components in Examples 1, 2 and 3 increased sequentially, which indicates that the killing effect on diseases increased sequentially.

[0060] Example 4 uses lecithin to coat essential oils. Due to the slow-release effect of the coating, the prevention and treatment effect is slightly improved. Therefore, Example 4 may be more suitable for cleaning that requires long-term protection or early prevention.

[0061] Examples 5, 6, 7, 8, and 9 used different essential oils or resins to replace the essential oils or resins specifically selected in this invention, or performed default tests. Table 1 shows that the performance varied due to the different effectiveness of different components against different pathogenic microorganisms, but without exception, none of them were as effective as the combination of monoterpene alcohol essential oils and monoterpene resins specifically selected in this invention.

[0062] Example 10 uses unfermented garlic cell sap instead of fermented garlic cell sap; the results show that fermentation can improve its prevention and control effect.

[0063] Example 11 uses purified water instead of fermented garlic cell broth, which lacks the bactericidal effect of fermented garlic cell broth and significantly reduces the effective concentration of other components, resulting in extremely low protective effect.

[0064] The above detailed description is a specific description of one of the feasible embodiments of the present invention. This embodiment is not intended to limit the patent scope of the present invention. All equivalent implementations or modifications that do not depart from the present invention should be included within the scope of the technical solution of the present invention.

Claims

1. A plant extract combination preparation, characterized in that, Ingredients included in parts by weight: 1-3 parts monoterpene alcohol essential oil 1-3 parts of monoterpene resin 0.2-0.4 parts of natural surfactant 10 portions of fermented cell broth The fermented cell broth is a product obtained by microbial fermentation of at least one of garlic cell broth, onion cell broth, and mustard cell broth. The microorganisms include at least one of the following: At least one of Lactobacillus rhamnosus, Bacillus amyloliquefaciens, and Bacillus subtilis.

2. The plant extract combination preparation according to claim 1, characterized in that, The monoterpene alcohol essential oil includes at least one of the following components: Linalool oil, geranium oil, and eucalyptus oil.

3. The plant extract combination preparation according to claim 1, characterized in that, The monoterpene resin comprises at least one of the following components: Liquidambar resin, rosin, and Cuban resin.

4. The plant extract combination preparation according to claim 1, characterized in that, The natural surfactant includes at least one of the following components: tea saponin, saponins, rhamnolipin, and sophorolipid.

5. The plant extract combination preparation according to claim 1, characterized in that, It also includes lecithin, the mass of which is at least one-third of the total mass of monoterpene essential oil and monoterpene resin.

6. The plant extract combination preparation according to claim 5, characterized in that, Lecithin was used to encapsulate monoterpene essential oils and monoterpene resins.

7. The plant extract combination preparation according to claim 1, characterized in that, The method for preparing fermented cell slurry is as follows: after crushing the plant raw material, filter it and take the filtrate as the sole nutrient source, and ferment it in a liquid state for 18-36 hours at a temperature of 25-40℃.

8. The application of the plant extract combination preparation according to any one of claims 1-7 in the prevention and control of citrus diseases.

9. The application of the plant extract combination preparation according to claim 8 in the prevention and control of citrus diseases, characterized in that, The citrus disease mentioned is at least one of citrus Huanglongbing, citrus canker, and citrus anthracnose.

10. The application of the plant extract combination preparation according to claim 8 in the prevention and control of citrus diseases, characterized in that, The medicine is applied by spraying or injection.

Images