A combination of calcium, iron, zinc, and multivitamins containing NFC lemon juice to synergistically promote absorption and aid growth.

Abstract

This invention belongs to the field of food technology and discloses a calcium, iron, zinc, and complex vitamin synergistic absorption-promoting and growth-aiding composition containing NFC lemon juice. The composition uses NFC lemon juice as the basic functional carrier and is compounded with specific organic minerals L-calcium lactate, zinc lactate, sodium ferric EDTA, and water-soluble vitamins (vitamin C and various B vitamins) to construct a synergistic absorption nutritional system. Through formula optimization and process innovation, this invention effectively improves the chelation rate of minerals, reduces the risk of product precipitation to ensure stability, achieves a synergistic absorption-promoting effect of minerals and vitamins, supports growth and development, and is suitable for industrial production needs.

Description

Technical Field

[0001] This invention belongs to the field of food technology, specifically relating to a composition containing NFC lemon juice and calcium, iron, zinc, and a complex vitamin that synergistically promotes absorption and aids growth. Background Technology

[0002] Calcium, iron, and zinc, as essential macro- and micro-minerals for the human body, are core nutrients supporting life activities and growth and development, and their physiological functions are irreplaceable: Calcium is a key component of bone and teeth mineralization, directly affecting bone density and bone development quality, while also participating in nerve signal transmission and muscle contraction; Iron is an important component of hemoglobin, myoglobin, and various enzymes, responsible for oxygen transport and energy metabolism, and its deficiency easily leads to iron-deficiency anemia, which in turn affects cognitive development and immunity; Zinc, as the active center of more than 300 enzymes, regulates protein synthesis, immune cell proliferation, and wound healing, and is crucial for the growth rate and disease resistance of children and adolescents. Meanwhile, the synergistic effect of vitamins and minerals has become a key scientific consensus for enhancing nutritional efficacy: Vitamin C can convert poorly absorbed ferric iron (Fe3+) into easily absorbed ferrous iron (Fe2+) through reduction, significantly improving iron bioavailability; B vitamins, as coenzymes, participate in the metabolic activation of minerals.

[0003] Chinese Patent Publication No. CN119033045A discloses a calcium, iron, and zinc oral liquid and its preparation method, comprising: soluble calcium, soluble iron, soluble zinc, stabilizer, natural sweetener, natural flavoring agent, emulsifier, chelating agent, inulin, and purified water. The bioavailability and absorption rate of calcium, iron, and zinc are improved by selecting a chelating agent. Chinese Patent Publication No. CN108925994A discloses an iron-zinc-calcium compound vitamin oral liquid, comprising: zinc inulin, ginseng extract, iron supplement, calcium oligosaccharide, taurine, Ganoderma lucidum extract, jujube seed extract, natural flavoring agent, sucrose invert sugar, and water.

[0004] However, existing calcium, iron, and zinc compound nutritional preparations (including beverage products) still face multiple bottlenecks in their implementation, severely restricting the realization of their nutritional efficacy and the improvement of product quality. These bottlenecks are specifically manifested in the following aspects:

[0005] (1) Low mineral absorption efficiency: Most existing products use inorganic minerals (such as calcium carbonate, ferrous sulfate, and zinc chloride) as raw materials. These minerals not only have low bioavailability, but may also irritate the gastrointestinal mucosa, causing discomfort such as bloating and diarrhea. At the same time, inorganic minerals are easy to combine with anti-nutritional factors such as phytic acid and oxalic acid in food, further reducing absorption efficiency and making it difficult to meet the nutritional needs of the target population (especially children and adolescents).

[0006] (2) Poor system stability and easy precipitation: When multiple minerals are mixed, different ions are prone to interaction, which leads to the product layering and precipitation. This not only affects the uniformity of appearance and consumer acceptance, but also causes uneven distribution of nutrients, making it impossible to guarantee the stable nutritional dosage of each sip of beverage. Some products add a large amount of food additives (such as stabilizers and emulsifiers) to improve stability. Although this can temporarily alleviate the precipitation problem, it increases health risks and does not meet the consumption demand for natural and healthy products.

[0007] Therefore, there is an urgent need to develop a composition that achieves "high mineral chelation rate and high system stability" through formula optimization and process innovation to synergistically promote absorption and growth, so as to meet the needs of industrial production and consumers' demand for natural and efficient nutritional beverages. Summary of the Invention

[0008] To overcome the technical bottlenecks in existing calcium, iron, and zinc compound preparations, such as the easy precipitation of minerals, this invention provides a calcium, iron, and zinc-complex vitamin composition containing NFC lemon juice that synergistically promotes absorption and growth. Through formula optimization and process innovation, it achieves a high chelation rate of minerals and synergistic effects of nutrients, while ensuring product appearance uniformity and suitability for industrial production.

[0009] This invention uses NFC lemon juice as the basic functional carrier, combined with organic minerals and water-soluble vitamins to form a precisely proportioned nutritional system. The specific formula is as follows:

[0010] Basic functional carrier: NFC lemon juice, as a natural medium for mineral chelation, contains pectin microparticles that can form a stable bond with calcium.

[0011] Organic minerals: We select organic minerals with high bioavailability, including L-calcium lactate (calcium source), zinc lactate (zinc source), and sodium iron ethylenediaminetetraacetate (iron source), avoiding the problems of traditional inorganic minerals such as easy precipitation and gastrointestinal irritation.

[0012] Water-soluble vitamins: including vitamin C (promotes iron reduction and absorption) and B vitamins (vitamin B1, vitamin B2, vitamin B6, niacinamide, vitamin B12, which participate in mineral metabolism activation), and water-soluble vitamin D3 powder (purchased from Jiaxing Fuweikang Biotechnology Co., Ltd.), to build a "mineral-vitamin" synergistic absorption system.

[0013] This invention solves the problems of insufficient mineral chelation, easy vitamin inactivation, and system instability through a multi-step, precisely controlled preparation process. The specific process steps are as follows:

[0014] NFC lemon juice pretreatment: Preserves active ingredients and ensures a sterile environment

[0015] Low-temperature centrifugation for impurity removal: Centrifuge at 8000-12000 r / min for 5-10 min at 15-25℃ to remove coarse fibers while retaining natural pectin microparticles.

[0016] Pulsed electric field sterilization: Sterilization is carried out using a 30kV / cm, 200μs pulsed electric field, which avoids vitamin destruction and flavor deterioration caused by traditional heat sterilization and ensures the sterility of the system.

[0017] Mineral stepwise chelation process

[0018] Overcoming the drawbacks of traditional "simultaneous addition and constant temperature stirring," a chelation process with step-by-step temperature control and speed-controlled stirring in the "low temperature-medium temperature-low temperature" stages is designed:

[0019] Calcium chelation stage: L-calcium lactate was added to lemon juice at an ultra-low temperature of 0-4℃ and sheared and stirred at 5000-8000 r / min for 25-45 min to promote the stable binding of calcium and pectin and reduce the free calcium content. To study the effect of ultra-low temperature + high-speed shearing, the inventors conducted a series of experiments. At 0-4℃, shearing and stirring at 2000 r / min for 35 min resulted in a calcium chelation rate of about 71%. At 15℃, shearing and stirring at 800 r / min for 35 min resulted in a calcium chelation rate of about 73%. At 15℃, shearing and stirring at 2000 r / min for 35 min resulted in a calcium chelation rate of about 75%.

[0020] Zinc chelation stage: Heat the system to 7-12℃ at a slow rate of ≤0.5℃ / min, add zinc lactate at 5-15min intervals, and stir at a medium speed of 600-1000r / min for 20-30min.

[0021] Iron chelation stage: Cool the system to 3-6℃, add sodium iron ethylenediaminetetraacetate, and stir at a low speed of 500-700r / min for 30-40min.

[0022] First, take a portion of the pretreated lemon juice, control the temperature at 15-25℃, add B vitamins, stir at 400-600r / min for 8-12min, add water-soluble vitamin D3 powder, continue stirring for 3-5min, add vitamin C and stir evenly, then slowly inject into the mineral chelation system, and finally add vitamin C to reduce vitamin C inactivation.

[0023] System homogenization and degassing: ensuring uniformity and reducing oxidation risk

[0024] Deep vacuum degassing: Degas for 10-20 minutes at 10-15℃ and -0.1MPa vacuum.

[0025] Two-stage high-pressure homogenization: First, the system is crushed by initial pressure of 20MPa, and then further refined by final pressure of 50MPa, while eliminating air bubbles to ensure that the product has a uniform appearance and no sediment.

[0026] Aseptic filling and packaging: extending shelf life and preserving nutritional activity

[0027] The product employs a sterile filling process of "nitrogen replacement + secondary nitrogen replenishment" and is sealed with double-layer light-proof packaging containers to prevent damage to vitamins and minerals from light and oxygen, thereby extending the product's shelf life while preserving the activity of nutrients.

[0028] Compared with the prior art, the technical advantages of the present invention are as follows:

[0029] (1) The mineral chelation rate is significantly improved, resulting in higher absorption efficiency.

[0030] This invention utilizes a stepwise chelation process, achieving chelation rates of over 85% for calcium, iron, and zinc, significantly higher than the control group. Chelated minerals are less affected by gastrointestinal pH and avoid binding with anti-nutritional factors in food, resulting in a substantial increase in bioavailability. The stepwise chelation technology of this invention essentially employs "precise temperature control, dynamic speed adjustment, and scientific sequencing" to allow calcium, iron, and zinc to form stable, highly absorbable chelates within the complex lemon juice system, while simultaneously preventing ion repulsion and system precipitation.

[0031] (2) The product has excellent stability and no risk of sedimentation.

[0032] Examples 1-3 have low precipitation rates and low pectin-bound calcium content, which fundamentally solves the problems of poor appearance and uneven nutrient distribution caused by mineral precipitation in traditional compound preparations, thus meeting the needs of industrial production and consumer acceptance. Attached Figure Description

[0033] Figure 1 Chelation rates of calcium, iron, and zinc. Detailed Implementation

[0034] To make the objectives and technical solutions of this invention clearer, the following embodiments are provided for further explanation. However, the scope of protection of this invention is not limited to these embodiments; the embodiments are merely for illustrative purposes. Those skilled in the art should understand that any changes or equivalent substitutions that do not depart from the concept of this invention are included within the scope of protection of this invention.

[0035] Example 1

[0036] formula:

[0037]

[0038] Preparation process:

[0039] (1) NFC lemon juice pretreatment upgrade

[0040] The process employs a low-temperature multi-stage filtration technique: 20℃ constant temperature centrifugation (10000r / min, 8min) removes coarse fibers while retaining natural pectin microparticles; pulsed electric field sterilization (30kV / cm, 200μs).

[0041] (2) Mineral stepwise chelation process

[0042] (1) First stage (calcium chelation): Add NFC lemon juice to a sterile mixing tank and maintain an ultra-low temperature environment of 2°C (tank wall jacket circulating refrigerant control). After adding L-calcium lactate, shear and stir at 6500r / min for 35min.

[0043] (2) Second stage (zinc chelation): After calcium chelation is completed, the system temperature is slowly raised to 10℃ (heating rate ≤ 0.5℃ / min), zinc lactate is added at 10min intervals, and the mixture is stirred at 800r / min for 25min.

[0044] (3) Third stage (iron chelation): After zinc chelation is completed, the system temperature is lowered to 4°C, ethylenediaminetetraacetic acid iron sodium is added, stirred at 600r / min for 35min, and then slowly heated to 8°C for storage.

[0045] (3) Addition of vitamins

[0046] a. Pretreatment: Take 80ml of NFC lemon juice, control the temperature at 20℃ (constant temperature water bath heating), add B vitamins (B2, B6, nicotinamide), stir at 500r / min for 10min, add water-soluble vitamin D3 powder, and continue stirring for 4min.

[0047] b. Mixing: Add vitamin C, stir for 10 minutes, and then slowly inject into the mineral chelation system.

[0048] (4) Optimization of system homogenization and degassing

[0049] a. Deep vacuum degassing: Degassing at -0.1MPa vacuum and 12℃ for 15 minutes reduces the oxygen content of the system to below 0.3mg / L, thus reducing oxidation loss.

[0050] b. Two-stage high-pressure homogenization: initial pressure of 20MPa breaks up agglomerated particles, and final pressure of 50MPa defoaming.

[0051] (5) Aseptic filling and packaging

[0052] Nitrogen purging, filling, secondary nitrogen replenishment, and sealing.

[0053] Example 2

[0054] formula:

[0055]

[0056] Preparation process:

[0057] (1) NFC lemon juice pretreatment upgrade

[0058] The process employs a low-temperature multi-stage filtration technique: centrifugation at 15℃ (8000r / min, 5min) removes coarse fibers while retaining natural pectin microparticles; and sterilization is performed using a pulsed electric field (30kV / cm, 200μs).

[0059] (2) Mineral stepwise chelation process

[0060] (1) First stage (calcium chelation): Add NFC lemon juice to a sterile mixing tank and maintain an ultra-low temperature environment of 0℃ (tank wall jacket circulating refrigerant control). After adding L-calcium lactate, shear and stir at 5000r / min for 25min.

[0061] (2) Second stage (zinc chelation): After calcium chelation is completed, the system temperature is slowly raised to 7℃ (heating rate ≤ 0.5℃ / min), zinc lactate is added at 5min intervals, and the mixture is stirred at 600r / min for 20min.

[0062] (3) Third stage (iron chelation): After zinc chelation is completed, the system temperature is lowered to 3°C, ethylenediaminetetraacetic acid iron sodium is added, stirred at 500r / min for 30min, and then slowly heated to 8°C for storage.

[0063] (3) Vitamin addition

[0064] a. Pretreatment: Take 80ml of NFC lemon juice, control the temperature at 15℃ (constant temperature water bath heating), add B vitamins (B2, B6, nicotinamide), stir at 400r / min for 8min, add water-soluble vitamin D3 powder, and continue stirring for 3min.

[0065] b. Mixing: Add vitamin C, stir for 10 minutes, and then slowly inject into the mineral chelation system.

[0066] (4) Optimization of system homogenization and degassing

[0067] a. Deep vacuum degassing: Degassing at -0.1MPa vacuum and 10℃ for 10 minutes reduces the oxygen content of the system to below 0.3mg / L.

[0068] b. Secondary high-pressure homogenization: 20MPa initial pressure to break up agglomerated particles, 50MPa final pressure treatment to defoam.

[0069] (5) Aseptic filling and packaging

[0070] Nitrogen purging, filling, secondary nitrogen replenishment, and sealing.

[0071] Example 3

[0072] formula:

[0073]

[0074] Preparation process:

[0075] (1) NFC lemon juice pretreatment upgrade

[0076] The process employs a low-temperature multi-stage filtration process: 25℃ constant temperature centrifugation (12000r / min, 10min) removes coarse fibers while retaining natural pectin microparticles; pulsed electric field sterilization (30kV / cm, 200μs).

[0077] (2) Mineral stepwise chelation process

[0078] (1) First stage (calcium chelation): Add NFC lemon juice to a sterile mixing tank and maintain an ultra-low temperature environment of 4°C (tank wall jacket circulating refrigerant control). After adding L-calcium lactate, shear and stir at 8000r / min for 45min.

[0079] (2) Second stage (zinc chelation): After calcium chelation is completed, the system temperature is slowly raised to 12℃ (heating rate ≤ 0.5℃ / min), zinc lactate is added at 15min intervals, and the mixture is stirred at 1000r / min for 30min.

[0080] (3) Third stage (iron chelation): After zinc chelation is completed, the system temperature is lowered to 6°C, ethylenediaminetetraacetic acid iron sodium is added, stirred at 700r / min for 40min, and then slowly heated to 8°C for storage.

[0081] (3) Vitamin addition

[0082] a. Pretreatment: Take 80ml of NFC lemon juice, control the temperature at 25℃ (constant temperature water bath heating), add B vitamins (B2, B6, nicotinamide), stir at 600r / min for 12min, add water-soluble vitamin D3 powder, and continue stirring for 5min.

[0083] b. Mixing: Add vitamin C, stir for 10 minutes, and then slowly inject into the mineral chelation system.

[0084] (4) Optimization of system homogenization and degassing

[0085] a. Deep vacuum degassing: Degassing at -0.1MPa vacuum and 15℃ for 20 minutes reduces the oxygen content of the system to below 0.3mg / L.

[0086] b. Secondary high-pressure homogenization: 20MPa initial pressure to break up agglomerated particles, 50MPa final pressure treatment to defoam.

[0087] (5) Aseptic filling and packaging

[0088] Nitrogen purging, filling, secondary nitrogen replenishment, and sealing.

[0089] Control group setup

[0090] Table 1 Control group design

[0091]

[0092] Determination of mineral chelation rate (calcium / iron / zinc)

[0093] The mineral chelation rate was determined by high performance liquid chromatography coupled with atomic absorption spectrometry.

[0094] Sedimentation rate determination

[0095] Pectin was precipitated using ethanol (80% by volume), removed by filtration, and the unchelated minerals in the system were separated by centrifugation. After drying to constant weight, the percentage of the precipitate mass to the total mineral mass in the sample was calculated, which directly reflects the optimization effect of the process on mineral solubility.

[0096] Determination of pectin-bound calcium content

[0097] The process of "ethanol precipitation of pectin → washing to remove free calcium → digestion of pectin to release bound calcium → atomic absorption quantification" was used to separate and determine calcium bound to pectin, and to quantify the effect of different chelation sequences on calcium-pectin binding.

[0098] Table 2 Mineral chelation rate

[0099]

[0100] The mineral chelation rate directly determines the "content of bioavailable active minerals" in the composition. Chelated minerals (such as calcium-citric acid chelates and EDTA-iron chelates) are less affected by the gastrointestinal environment and have a higher absorption rate than free minerals. Table 2 provides a quantitative basis for whether the process can "enhance nutritional efficacy" by accurately measuring the chelation rates of calcium, iron, and zinc. Experimental data show that the calcium, iron, and zinc chelation rates in Examples 1-3 are significantly higher than those in all control groups, proving that the stepwise chelation process of the present invention, characterized by "low temperature-medium temperature-low temperature," can accurately match the chelation characteristics of the three minerals.

[0101] Table 3. Sedimentation rate and pectin-bound calcium content

[0102]

[0103] If precipitation occurs in the composite product, it not only affects the appearance (leading to decreased consumer acceptance) but also results in uneven mineral distribution. Pectin-bound calcium is the main source of precipitation in the NFC lemon juice system (free calcium readily binds to pectin carboxyl groups to form insoluble complexes). Table 3 provides quantitative evidence of whether the process can "ensure product stability and meet the needs of industrial production" by measuring the precipitation rate and pectin-bound calcium content. Experimental results show that the precipitation rate in Examples 1-3 is less than 6.0%, and the pectin-bound calcium content is less than 6.4 mg / 1000 mL, far lower than the control group. This proves that the "stepwise chelation process" improves the mineral chelation rate, reduces the non-specific binding of free minerals to pectin and iron oxidation precipitation, thus ensuring system stability from the root.

[0104] Changes in the molecular weight of pectin

[0105] The weight-average molecular weight (Mw) of pectin was determined using size exclusion chromatography coupled with multi-angle laser light scattering.

[0106] Experimental setup: Five shearing conditions were set up (control group + 4 experimental groups), with each group repeated in triplicate.

[0107] Control group (CK): No shearing treatment, only stirring and mixing (200 r / min, 25 min).

[0108] Experimental group 1 (E1): 5000 r / min, 25 min.

[0109] Experimental group 2 (E2): 6500 r / min, 35 min.

[0110] Experimental group 3 (E3): 8000 r / min, 45 min.

[0111] Experimental group 4 (E4): 10000 r / min, 45 min.

[0112] Initial baseline: The initial Mw of pectin in NFC lemon juice is 238 ± 7 kDa.

[0113] Table 4 Experimental data on changes in pectin molecular weight

[0114]

[0115] Table 4 shows that as the shear rate increases and the time increases, the pectin Mw decreases, but the degradation range is controllable within the parameter range of 5000-8000 r / min (E1-E3), and there is no excessive degradation, and the core structure of the long pectin chain is still retained.

Claims

1. A synergistically absorbable growth promoting composition of calcium iron zinc- complex vitamins with NFC lemon juice characterized in that, The formula of the composition is: NFC lemon juice, calcium L-lactate, zinc lactate, iron sodium ethylenediaminetetraacetate, vitamin C, water-soluble vitamin D3 powder, B vitamins; The preparation process of the NFC lemon juice-containing calcium-iron-zinc-multivitamin synergistic absorption-promoting growth composition is as follows: (1) NFC lemon juice pretreatment: remove coarse fibers by centrifugation at 8000-12000 r / min and constant temperature for 5-10 min at 15-25 ℃, and then sterilize by pulse electric field; (2) Mineral step-by-step chelation: first step: calcium chelation: add the NFC lemon juice treated in step (1) into a sterile stirring tank, maintain an ultralow temperature environment of 0-4 ℃, and after adding calcium L-lactate, shear and stir at a rate of 5000-8000 r / min for 25-45 min; second step: zinc chelation: after completing calcium chelation, slowly raise the system temperature to 7-12 ℃ at a rate of ≤0.5 ℃ / min, add zinc lactate at intervals of 5-15 min, and stir at a rate of 600-1000 r / min for 20-30 min; third step: iron chelation: after completing zinc chelation, reduce the system temperature to 3-6 ℃, add iron sodium ethylenediaminetetraacetate, and stir at a rate of 500-700 r / min for 30-40 min, and slowly raise the temperature to 8 ℃ for storage; (3) Vitamin addition: first take the NFC lemon juice treated in step (1), control the temperature at 15-25 ℃, add B vitamins and stir at a rate of 400-600 r / min for 8-12 min, add water-soluble vitamin D3 powder, add vitamin C, and slowly inject the mineral chelation system obtained in step (2) after stirring; (4) System treatment: vacuum degassing and high-pressure homogenization; (5) Packaging. The preparation process comprises the following steps: (1) NFC lemon juice pretreatment: remove coarse fibers by centrifugation at 8000-12000 r / min and constant temperature for 5-10 min at 15-25 ℃, and then sterilize by pulse electric field; 2. The calcium iron zinc-complex vitamin synergistically absorption-facilitating growth- promoting NFC lemon juice containing composition as claimed in claim 1, wherein, The B vitamins are vitamin B2, vitamin B 6、 niacinamide, vitamin B1, vitamin B 12 one or more of.

3. The calcium iron zinc-complex vitamin synergistically absorbable growth promoting composition with NFC lemon juice as claimed in claim 1, wherein, The composition is formulated with 1000 ml of NFC lemon juice, 100-350 mg of calcium L-lactate, 15-65 mg of zinc lactate, 20-80 mg of sodium iron ethylenediaminetetraacetate, 80-120 mg of vitamin C, 0.5-1.5 mg of vitamin B2, 0.1-0.25 mg of vitamin B6, 1-2 mg of nicotinamide, 0.1-0.3 mg of vitamin B1, 0.1-0.3 mg of vitamin B6, 0.1-0.3 mg of vitamin B12, 0.2-0.5 μg of water-soluble vitamin D3 powder, and 20-100 μg of water-soluble vitamin D3 powder. 12 0.2-0.5 μg of water-soluble vitamin D3 powder, and 20-100 μg of water-soluble vitamin D3 powder.

4. The calcium iron zinc-complex vitamin synergistically absorbable growth promoting composition with NFC lemon juice according to claim 1, characterized by, The formulation of the composition is: NFC lemon juice 1000 ml, calcium L-lactate 250 mg, zinc lactate 35 mg, iron sodium ethylenediaminetetraacetate 60 mg, vitamin C 100 mg, vitamin B2 1.2 mg, vitamin B6 0.15 mg, nicotinamide 1.5 mg, vitamin B1 0.2 mg, vitamin B 12 0.3 μg, water-soluble vitamin D3 powder 60 μg.

5. The calcium iron zinc-complex vitamin synergistically absorbable growth promoting composition with NFC lemon juice as claimed in claim 1, wherein, (2) Mineral step-by-step chelation: first step: calcium chelation: add the NFC lemon juice treated in step (1) into a sterile stirring tank, maintain an ultralow temperature environment of 0-4 ℃, and after adding calcium L-lactate, shear and stir at a rate of 5000-8000 r / min for 25-45 min; second step: zinc chelation: after completing calcium chelation, slowly raise the system temperature to 7-12 ℃ at a rate of ≤0.5 ℃ / min, add zinc lactate at intervals of 5-15 min, and stir at a rate of 600-1000 r / min for 20-30 min; third step: iron chelation: after completing zinc chelation, reduce the system temperature to 3-6 ℃, add iron sodium ethylenediaminetetraacetate, and stir at a rate of 500-700 r / min for 30-40 min, and slowly raise the temperature to 8 ℃ for storage; (3) Vitamin addition: first take the NFC lemon juice treated in step (1), control the temperature at 15-25 ℃, add B vitamins and stir at a rate of 400-600 r / min for 8-12 min, add water-soluble vitamin D3 powder, add vitamin C, and slowly inject the mineral chelation system obtained in step (2) after stirring; ​ ​ (4) System degassing and homogenization: vacuum degassing at 10-15℃, and then secondary high pressure homogenization; (5) Sterile filling and packaging: filling, and selecting double-layer light-proof packaging container for sealing.

Images