Fermentation product of lavender flower and camellia seed oil as well as preparation method and application of fermentation product

Abstract

The invention relates to the field of biological medicine, and discloses a fermentation product of lavender flower and camellia seed oil as well as a preparation method and application of the fermentation product. The preparation method of the fermentation product of the lavender flowers and the camellia seed oil comprises the following steps: fermenting a flower oil mixture by adopting Starmomyces bumblebee, and taking an oil-phase product after fermentation; the flower oil mixture is a mixture of lavender flowers and camellia seed oil. According to the method, the essential oil in the lavender flowers can be efficiently extracted, and the mixed product of the fermented lavender flowers and the camellia seed oil has a synergistic effect in efficacy, has good anti-inflammatory and moisturizing effects, and can be applied to anti-inflammatory products and moisturizing products.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of biological medicine, in particular to a fermentation product of lavender flowers and camellia seed oil, and a preparation method and application thereof. BACKGROUND

[0002] Lavender is rich in aromatic essential oil in its flowers, and has high ornamental and application values. At present, the lavender resources are mainly used by adopting an organic solvent extraction method, a steam distillation method or a supercritical carbon dioxide extraction method to extract lavender essential oil from the lavender flowers, so as to be widely used in cosmetics, washing products and food as a fragrance raw material. According to some reports, the lavender essential oil has effects of inhibiting bacteria, resisting inflammation, resisting oxidation, inhibiting sebum secretion, inhibiting tyrosinase activity, synthesizing collagen and promoting hair growth. However, in the current popular extraction process, the organic solvent extraction method needs to use volatile organic solvents such as n-hexane, and has risks of solvent residue and environmental pollution; the steam distillation process needs high energy consumption, and produces a large amount of distillation wastewater; and the supercritical carbon dioxide extraction method needs high equipment investment and high production cost. How to greenly and efficiently extract the lavender essential oil from the lavender flowers is a challenging work.

[0003] In view of this, the present application is provided. SUMMARY

[0004] The present application aims to provide a fermentation product of lavender flowers and camellia seed oil, and a preparation method and application thereof, and aims to improve at least one problem mentioned in the background art.

[0005] The present application is implemented in the following manner: In a first aspect, the present application provides a preparation method of a fermentation product of lavender flowers and camellia seed oil, comprising the following steps: The flower oil mixture is fermented by using the Bombicola thesii, and an oil phase product is taken after fermentation; the flower oil mixture is a mixture of the lavender flowers and the camellia seed oil.

[0006] In an optional embodiment, the lavender flowers are dry lavender flowers, and the mass ratio of the dry lavender flowers to the camellia seed oil is 1:5-20.

[0007] In an optional embodiment, the Bombicola thesii is classified and named as: Bombicola thesii RTNY120013 Starmerella bombicola RTNY120013, which is preserved in the China Center for Type Culture Collection, Wuhan University, Wuhan, China, on August 12, 2025, and has a preservation number of CCTCC NO:M 20251820.

[0008] In an optional embodiment, the fermentation mode comprises the following steps: The OD600nm The Bombiostamo yeast fermentation liquor and the flower oil mixture are mixed and fermented for 40-56 hours, and the volume ratio of the Bombiostamo yeast fermentation liquor and the flower oil mixture is 1-5:1-5.

[0009] In optional embodiments, at least one of the following features (1) and (2) is included: (1) the OD 600nm Before the Bombiostamo yeast fermentation liquor and the flower oil mixture are mixed and fermented, the following steps are further included: The Bombiostamo yeast seed liquid is cultured in the sterilized YPD medium, and the culture is performed until the OD 600nm ≥10; The yeast seed liquid is inoculated into the sterilized YPD medium, and the culture is performed until the OD 600nm ≥50 to obtain the Bombiostamo yeast fermentation liquor; (2) the volume ratio of the Bombiostamo yeast fermentation liquor and the flower oil mixture is 1-4:1-2, or 1:2.

[0010] In optional embodiments, at least one of the following features (1) and (2) is included: (1) the lavender flowers in the flower oil mixture are dry lavender flower powders passing through a 100-mesh screen; (2) the lavender flowers are dry lavender flowers, and the mass ratio of the dry lavender flowers and the camellia seed oil is 1:8-15, or 1:10-12, or 1:10.

[0011] In a second aspect, the present application provides a fermentation product of lavender flowers and camellia seed oil, which is prepared by the method according to any one of the preceding embodiments.

[0012] In a third aspect, the present application provides a moisturizing product, which comprises the fermentation product of lavender flowers and camellia seed oil according to the preceding embodiments.

[0013] In optional embodiments, the content of the fermentation product is 0.1-3%wt.

[0014] In a fourth aspect, the present application provides an anti-inflammatory product, which comprises the fermentation product of lavender flowers and camellia seed oil according to the preceding embodiments.

[0015] The present application has the following beneficial effects: The bumblebee s. bombicola in the present application can effectively degrade the plant fiber and plant cell wall of lavender flower by producing various hydrolytic enzymes, and has a certain hydrolysis effect on oil (camellia seed oil), improves the affinity of oil (camellia seed oil) to plant tissue (lavender flower), and the combination of the two effects can promote the permeability of oil (camellia seed oil) to plant cells (lavender flower), so that the release rate of lavender flower essential oil is significantly improved. The present application combines the plant fermentation technology of water phase and the plant extraction technology of oil phase together to obtain a method for efficiently obtaining a fermentation composition of lavender flower and camellia seed oil. It is verified through experiments that the mixed fermentation of lavender flower and camellia seed oil has a synergistic effect, the product has good anti-inflammatory effect and good moisturizing effect, and is very suitable for application in anti-inflammatory products or moisturizing products. BRIEF DESCRIPTION OF DRAWINGS

[0016] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings needed in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of the present application, and therefore should not be regarded as a limitation on the scope. For those skilled in the art, other related drawings can also be obtained without creative labor on the basis of these drawings.

[0017] Figure 1 Comparison of cytotoxicity of Examples 1-6 on RAW264.7 cells; Figure 2 Comparison of cytotoxicity of Example 1 and Comparative Examples 1-3 on RAW264.7 cells; Figure 3 Comparison of the ability of Examples 1-6 to inhibit NO expression by immune cells; Figure 4 Comparison of the ability of Example 1 and Comparative Examples 1-3 to inhibit NO expression by immune cells; Figure 5 Cytotoxicity of Example 1 and Comparative Examples 1-3 on HaCaT cells; Figure 6 Comparison of the ability of Example 1 and Comparative Examples 1-3 to promote AQP-3 expression by HaCaT cells; Figure 7 Ability of samples to promote the increase of water content in the stratum corneum of the skin; Figure 8 Ability of samples to reduce water loss from the skin. DETAILED DESCRIPTION

[0018] In order to make the purposes, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application will be clearly and completely described below. If specific conditions are not indicated in the embodiments, conventional conditions or the conditions recommended by manufacturers are adopted. If the manufacturers of the reagents or instruments are not indicated, the reagents or instruments are conventional products that can be purchased in the market.

[0019] The features and performances of the present application will be further described in detail below in combination with the embodiments.

[0020] Based on the problems in the prior art, the following thinking is made: Oil is an ester substance formed by fatty acids and glycerol, is a good solvent for natural plant essential oil, and is an important basic raw material for cosmetics. By using oil, especially oil from plants, to extract aromatic plants, natural plant essential oil and natural oil can be organically combined, organic solvent residues can be avoided, industrial wastewater can be reduced, and the extraction equipment and process are simple. However, plant tissues and cell walls are composed of hydrophilic materials such as cellulose, lignin and pectin, which are not compatible with oil, so that the permeability of oil to plant cells is poor, and the extraction efficiency of intracellular plant essential oil is low. Therefore, how to improve the extraction efficiency of oil for plant essential oil is a difficulty in the process of essential oil extraction.

[0021] In view of the above thinking, the present application is proposed: The present application provides a preparation method of a fermentation product of lavender flowers and camellia seed oil, comprising: The mixture of flower oil is fermented by using Bombiastarmo yeast, and the oil phase product is taken after fermentation; the mixture of flower oil is a mixture of lavender flowers and camellia seed oil.

[0022] Plant fermentation technology is a method to help oil extract plant essential oil efficiently. In the present application, Bombiastarmo yeast can effectively degrade plant fibers and plant cell walls of lavender flowers by producing various hydrolytic enzymes, and also has a certain hydrolysis effect on oil (camellia seed oil), thereby improving the affinity of oil (camellia seed oil) to plant tissues (lavender flowers). The combination of the two effects can promote the permeability of oil (camellia seed oil) to plant cells (lavender flowers), so that the release rate of lavender flower essential oil is significantly improved. The present application combines plant fermentation technology in water phase and plant extraction technology in oil phase together to obtain a method for efficiently obtaining a fermentation composition of lavender flowers and camellia seed oil. It is verified by experiments that the mixed fermentation of lavender flowers and camellia seed oil has a synergistic effect, and the product has good anti-inflammatory effect and good moisturizing effect, which is very suitable for application in anti-inflammatory products or moisturizing products.

[0023] Alternatively, the classification name of the Bombiastarmo yeast used for fermentation is: Bombiastarmo yeast RTNY120013 Starmerella bombicolaRTNY120013, deposited in China Center for Type Culture Collection, Wuhan University, Wuhan, China, on August 12, 2025, and assigned accession number CCTCC NO: M 20251820.

[0024] Specifically, the preparation method is: S1, using YPD medium to culture the Bombus sphaeroides stam yeast seed liquid, and culturing until OD 600nm ≥10.

[0025] Yeast activation and fermentation medium (YPD medium): Yeast powder 10 g / L, peptone 20 g / L, glucose 20 g / L, add water to 1000 mL, pH 6.5, sterilize at 121°C for 20 min.

[0026] S2, grinding the lavender dry flower and passing through a 100 mesh sieve, then uniformly mixing with camellia seed oil at a mass ratio (flower: oil) of 1:5-20, then sterilizing at 105°C for 15 min to obtain a sterilized flower oil mixture.

[0027] Preferably, the flower oil ratio is 1:8-15, further 1:10-12, and more preferably 1:10.

[0028] S3, preparing YPD medium and sterilizing at 120°C for 15 min, then cooling to 30°C, inoculating the Bombus sphaeroides stam yeast seed liquid at an inoculation amount of 5% (volume ratio), and culturing until OD 600nm ≥50, to obtain a yeast fermentation liquid.

[0029] S4, adding the sterilized flower oil mixture obtained in S2 to the Bombus sphaeroides stam yeast fermentation liquid obtained in S3, and continuing to ferment for 40-56 h, wherein the volume ratio of the Bombus sphaeroides stam yeast fermentation liquid to the sterilized flower oil mixture is 1-5:1-5.

[0030] Preferably, the volume ratio of the Bombus sphaeroides stam yeast fermentation liquid to the sterilized flower oil mixture is 1-4:1-2, and more preferably 1:2.

[0031] S5, after the fermentation is terminated, separating the fermentation product into layers, and retaining the upper oil phase.

[0032] S6, filtering and centrifuging the oil phase to remove impurities and dewater, to obtain the desired fermented product of lavender flower and camellia seed oil.

[0033] The method provided by the present application realizes the synergistic fermentation of camellia seed oil and lavender flowers by organically combining the plant fermentation technology of the water phase and the plant extraction technology of the oil phase, and improves the extraction efficiency of the lavender essential oil. The extraction process is green and efficient, and does not require large equipment investment and high energy consumption. The product has significant anti-inflammatory and moisturizing effects, and can be applied to high-efficiency moisturizing cosmetics.

[0034] The fermentation product of lavender flowers and camellia seed oil provided by the embodiment of the present application is prepared by the preparation method provided by the present application.

[0035] The moisturizing product provided by the embodiment of the present application comprises the fermentation product of lavender flowers and camellia seed oil provided by the embodiment of the present application.

[0036] Optionally, in order to make the moisturizing product have better moisturizing effect at a lower cost, the content of the fermentation product in the moisturizing product is 0.1-3%wt.

[0037] The anti-inflammatory product provided by the embodiment of the present application comprises the fermentation product of lavender flowers and camellia seed oil provided by the embodiment of the present application.

[0038] Embodiment 1 1. YPD medium was used to culture the Bombiostroma seed liquid (preserved in China Center for Type Culture Collection, preservation number CCTCC NO: M 20251820), and the culture was carried out until OD 600nm was 15; 2. The lavender dry flowers were crushed and passed through a 100-mesh sieve, then mixed with camellia seed oil at a mass ratio (flowers:oil) of 1:10, and then sterilized at 105℃ for 15 min to obtain a sterilized flower-oil mixture; 3. YPD medium was prepared and sterilized at 120℃ for 15 min, and then cooled to 30℃, and then inoculated with the Bombiostroma seed liquid at an inoculation amount of 5% (volume ratio), and the culture was carried out until OD 600nm was 65, to obtain a yeast fermentation liquid; 4. The sterilized flower-oil mixture obtained in step 2 was added to the Bombiostroma fermentation liquid obtained in step 3, and the fermentation was continued for 48 h, and the volume ratio of the sterilized flower-oil mixture to the yeast fermentation liquid was 1:2; 5. After the fermentation was terminated, the fermentation product was separated into two layers, and the upper oil phase was retained; 6. The oil phase was filtered to remove impurities and subjected to centrifugal dewatering treatment to obtain the fermentation product (fermentation composition) of lavender flowers and camellia seed oil.

[0039] Embodiment 2 This embodiment is basically the same as embodiment 1, and the only difference is that the mass ratio of the lavender dry flowers to the camellia seed oil is 1:5.

[0040] Example 3 This example is basically the same as Example 1, except that the mass ratio of lavender dry flower to camellia seed oil is 1:20.

[0041] Example 4 This example is basically the same as Example 1, except that the volume ratio of bumblebee starmo yeast fermentation liquor to flower oil mixture is 1:5.

[0042] Example 5 This example is basically the same as Example 1, except that the volume ratio of bumblebee starmo yeast fermentation liquor to flower oil mixture is 5:1.

[0043] Example 6 This example is basically the same as Example 1, except that the bumblebee starmo yeast (CGMCC 2.3667) sold by the China General Microbiological Culture Collection Center is used to replace the bumblebee starmo yeast in Example 1.

[0044] Comparative Example 1 The camellia seed oil used as a raw material in Example 1 is not subjected to fermentation treatment.

[0045] Comparative Example 2 This comparative example is basically the same as Example 1, except that the flower oil mixture in Example 1 is replaced with an equal amount of camellia seed oil.

[0046] Comparative Example 3 This comparative example is basically the same as Example 1, except that, after obtaining the sterilized flower oil mixture, an equal amount of pure water is used to replace the YPD culture medium without inoculating microorganisms, and stirring is continued for 48 h.

[0047] Experimental Example 1 Anti-inflammatory efficacy comparison: (1) Immune cell cytotoxicity test Mouse monocyte macrophages (RAW264.7) are commonly used model cells for verifying the expression of inflammatory factors by immune cells. Succinate dehydrogenase in the mitochondria of living cells can reduce exogenous MTT (thiazolyl blue) to water-insoluble blue-violet formazan crystals and deposit them in the cells. The lower the cell viability, the poorer this function. Therefore, the influence of different drugs on cell viability can be determined by the MTT test method, thereby characterizing the toxicity of the drugs to the cells.

[0048] The concentration of RAW264.7 cells in the logarithmic growth phase was adjusted to 10 4Cells / mL: 100 μL of cell suspension was added to each well of a 96-well plate and cultured for 12 hours. A certain amount of sample solution diluted with DMSO was added to each well, and the plates were cultured for another 12 hours. The culture medium was then completely discarded. Subsequently, culture medium containing 0.5 mg / mL MTT was added to each well, and the cells were cultured for another 3-5 hours until crystals formed. The culture medium was then completely discarded, and 150 μL of DMSO was added to each well. The plates were gently shaken at room temperature for 5 minutes to completely dissolve the crystals. The 96-well plate was then quickly removed, and the absorbance of each well was measured at 490 nm using a microplate reader.

[0049] Experimental results: (e.g.) Figure 1 As shown, by examining Examples 1-6 under different fermentation process conditions, it can be found that each example exhibits essentially no cytotoxicity at concentrations of 0.01% and below, and cell viability can be maintained above 80%. Figure 2 As shown, by examining Example 1 and Comparative Examples 1-3, it can be found that Example 1 and Comparative Examples 1-3 exhibit essentially no cytotoxicity at concentrations of 0.01% and below, and cell viability can be maintained above 80%. Based on the above cytotoxicity test results, tests were conducted to inhibit the expression of inflammatory factors in immune cells.

[0050] (2) Inhibit NO expression in immune cells NO is an important inflammatory signaling molecule, and its secretion level can reflect the intensity of inflammation. RAW264.7 macrophages secreted 2 × 10⁻⁶ NO molecules. 4 Seeds were planted at a density of 2 mL / well in 6-well plates and incubated for 18 h in a 5% CO2, 37°C biochemical incubator. The culture medium was aspirated and residual medium was washed away with PBS. 2 mL of DMEM was added to the blank group, 1 μg / mL LPS prepared in DMEM was added to the control group, and 1 μg / mL LPS prepared in DMEM and sample solution diluted with DMSO were added to the sample groups. All samples were co-incubated for 24 h. The supernatant was collected, centrifuged at 13000 rpm for 5 min, and the supernatant was collected. Following the manufacturer's instructions, the effect of each sample on LPS-induced inflammatory cytokine expression was determined using a NO detection kit.

[0051] Experimental results: such as Figure 3As shown, Example 1 has a significant ability to inhibit the expression of inflammatory factor NO by cells in the range of 0.001-0.01% relative to the control (Control), and has anti-inflammatory efficacy. In the same concentration (0.01%), the anti-inflammatory efficacy of Example 1 is better than that of Examples 2-6, and in particular, Example 1 is better than Example 6, which illustrates that the product fermented from the new Bombicola thesata m strain preserved in China Center for Type Culture Collection with the accession number CCTCC NO: M 20251820 has better anti-inflammatory effect than the product fermented from the existing Bombicola thesata m strain. As Figure 4 As shown, in the same concentration (0.00001%), the anti-inflammatory efficacy of Example 1 is better than that of Comparative Examples 1-3.

[0052] Experimental Example 2 Moisturizing efficacy comparison: (1) Keratinocyte toxicity test Keratinocytes (HaCaT) are commonly used model cells for verifying skin efficacy. Succinate dehydrogenase in the mitochondria of living cells can reduce exogenous MTT (thiazolyl blue) to water-insoluble blue-violet formazan crystals and deposit them in the cells. The lower the cell viability, the worse this function. Therefore, the influence of different drugs on cell viability can be determined by MTT assay, thereby characterizing the toxicity of the drugs to the cells.

[0053] The concentration of HaCaT cells in the logarithmic growth phase was adjusted to 10 4 cells / mL using DMEM medium, and 100 μL of the cell suspension was sequentially taken and added to a 96-well plate for culture for 12 hours; a certain amount of sample solution diluted with DMSO was added to each well, and the culture was continued for 12 hours, and then the culture medium was completely discarded, and then 0.5 mg / mL MTT-containing medium was added to each well, and the cells were cultured for 3-5 h, and then the crystals were generated; the culture medium was completely discarded, 150 μL of DMSO was added to each well, and the 96-well plate was quickly taken out, and the absorbance value of each well was determined at a wavelength of 490 nm using an enzyme-labeled instrument.

[0054] Experimental results: As shown in Figure 5 Example 1 and Comparative Examples 1-3 have no cytotoxicity at a concentration of 0.01% or less, and the cell viability can be maintained at more than 80%. Based on the test results of the above cytotoxicity, the test of promoting AQP-3 expression of HaCaT cells was carried out.

[0055] (2) Promoting AQP-3 expression of keratinocytes Aquaporin 3 (AQP-3) is a recognized functional protein for maintaining skin moisturizing ability, belonging to the aqua-glycerol channel protein subfamily, and having the function of transporting small molecules such as water, glycerol and urea across the membrane. Promoting the expression of AQP-3 in skin cells can achieve effective moisturizing of the skin.

[0056] Lysis solution preparation: 10 μL PMSF was added to 1 mL strong RIPA lysis solution and mixed evenly.

[0057] HaCaT cells were grown in complete medium (high glucose DMEM medium containing 10% FBS and 1% double-antibiotic). The cells were trypsinized for 6 min (according to the trypsin activity, the cells were digested until visible cell shedding), and inoculated in a 6-well plate at 2×10 5 cells / well, 2 mL per well, and cultured in a 37 ℃, 5% CO2 incubator for 18 h. The culture medium was discarded, washed with PBS for 3 times, 2 mL of 25 μg / mL SDS aqueous solution was added (blank group was not treated), and incubated for 4 h, and then washed with PBS for 3 times. Subsequently, 2 mL of DMEM medium was added to the control group (Control), and 2 mL of sample solution diluted with DMSO and DMEM medium was added to the sample group, and incubated in the incubator for 24 h. Finally, the culture solution was removed, washed with pre-cooled PBS for 2-3 times, 100 μL of lysis solution was added to each well, and the lysis solution was allowed to fully contact the cells on ice for 5-10 seconds. The lysis solution was collected, centrifuged at 10,000 rpm for 10 min, and the supernatant was used for ELISA detection of AQP-3 level.

[0058] Experimental results: as Figure 6 shown, compared with the blank group, the expression amount of AQP-3 in the HaCaT cells after SDS damage in the control group was significantly reduced. The sample of Example 1 had obvious performance in restoring and improving the expression of AQP-3 in HaCaT cells, and showed a dose-dependent relationship. In addition, under the same addition amount (0.01%), the ability of Example 1 to promote the expression of AQP-3 in cells was significantly better than that of Comparative Examples 1-3.

[0059] Experimental Example 3 (1) Water (blank group), fermented composition (Example 1), camellia seed oil (Comparative Example 1), fermented camellia seed oil (Comparative Example 2), and camellia seed oil extract of lavender flower (Comparative Example 3) were used as functional components to prepare moisturizing cream for human moisturizing efficacy test. The moisturizing cream formula is shown in Table 1.

[0060] Table 1 Moisturizing cream formula

[0061] (2) Moisturizing effect of moisturizing cream containing fermented lavender flower and camellia seed oil composition on human body The moisturizing effect of the cosmetic on human body can be verified by testing the water content of the stratum corneum and the amount of transdermal water loss.

[0062] Mark the measurement area on the inner side of the forearm of the subject. Before the formal test, the subject should sit quietly in a room that meets the standard for at least 30 minutes, with the forearm exposed and placed in the test state, and remain relaxed. Mark 3x3 cm on the inner side of the forearm during the experiment 2 The experimental area, multiple areas can be marked on the same arm, with a 1 cm interval between areas. Then apply about 0.02 g of moisturizing cream containing 2% water, fermented lavender flower and camellia seed oil composition (Example 1), fermented camellia seed oil (Comparative Example 2), unfermented camellia seed oil (Comparative Example 1), and camellia seed oil extract of lavender flower (Comparative Example 3) on different areas on the inner side of the forearm, respectively, and use a latex glove to evenly apply the test sample to the experimental area. Test the water content of the stratum corneum and the amount of transdermal water loss using a multifunctional skin detector before application (0h), 0.5h after application, 2h after application, and 4h after application, respectively.

[0063] The experimental results are shown in Tables 1 and 2. Figure 7 and Figure 8 As shown in Tables 1 and 2, the moisturizing ability of the moisturizing cream containing oil is significantly improved compared to the sample with water. The moisturizing cream containing fermented lavender flower and camellia seed oil composition (Example 1) has the best effect of increasing the water content of the stratum corneum, and also has the greatest ability to inhibit the loss of skin moisture.

[0064] In summary, the preparation method provided by the embodiments of the present application ferments lavender flowers and camellia seed oil using Bombostroma yeast, and the mixed fermentation of lavender flowers and camellia seed oil has a synergistic effect. The product has good anti-inflammatory effect and good moisturizing effect, and can be applied in anti-inflammatory products or moisturizing products.

[0065] The above is only a preferred embodiment of the present application and is not intended to limit the present application. For those skilled in the art, the present application can have various modifications and changes. Any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application shall be included in the protection scope of the present application.

Claims

1. A method for preparing a fermentation product of lavender flowers and sacha inchi oil, characterized by, Comprising: fermenting a flower oil mixture with Starmerella bombicola, and taking the oil phase product after fermentation; the flower oil mixture is a mixture of lavender flowers and camellia seed oil.

2. The production method according to claim 1, characterized by, the lavender flowers are dried lavender flowers, and the mass ratio of the dried lavender flowers to the camellia seed oil is 1:5-20.

3. The preparation method according to claim 1, characterized in that, The classification name of the Bombiostroma yeast is: Bombiostroma RTNY120013 Starmerella bombicola RTNY120013, deposited in the China Center for Type Culture Collection, Wuhan University, Wuhan, China, on August 12, 2025, and assigned accession number CCTCC NO: M 20251820.

4. The method of claim 1, wherein, the fermentation method comprises: OD 600nm The bumblebee S. bombicola fermentation broth with OD≥50 is mixed with the flower oil mixture, and the volume ratio of the bumblebee S. bombicola fermentation broth to the flower oil mixture is 1-5:1-5, and then the mixture is fermented for 40-56 h.

5. The preparation method according to claim 4, characterized in that, comprising at least one of the following features (1) and (2): (1) mixing the OD 600nm ≥ 50 of the bumblebee S. boulardii fermentate with the floral oil mixture prior to the mixed fermentation The seed liquid of B. clausii was cultured in sterilized YPD medium, and the culture was cultured to OD 600nm ≥ 10; The yeast seed liquid is inoculated into sterilized YPD medium and cultured to OD 600nm ≥50 to obtain the bumblebee Bstam yeast fermentation liquid; (2) the volume ratio of the Starmerella bombicola fermentation liquor to the flower oil mixture is 1-4:1-2, or 1:

2.

6. The method of claim 1, wherein, comprising at least one of the following features (1) and (2): (1) the lavender flowers in the flower oil mixture are dried lavender flower powders passing through a 100-mesh sieve; (2) the lavender flowers are dried lavender flowers, and the mass ratio of the dried lavender flowers to the camellia seed oil is 1:8-15, or 1:10-12, or 1:

10.

7. A fermented product of Lavandula angustifolia and Camellia seed oil, characterized in that, prepared by the preparation method according to any one of claims 1-6.

8. A moisturizing product characterized in that, the fermentation product of the lavender flowers and the camellia seed oil according to claim 7.

9. The moisturizing product of claim 8, wherein, the content of the fermentation product is 0.1-3%wt.

10. An anti-inflammatory product, characterized in that, the fermentation product of the lavender flowers and the camellia seed oil according to claim 7.

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