Plant composition for activating hair follicles and promoting hair growth and preparation method thereof

Abstract

The invention provides a plant composition for activating hair follicles and promoting hair growth and a preparation method thereof. The composition provided by the invention comprises (a) 0.9-1.1 parts by weight of ginseng extract, 0.9-1.1 parts by weight of cacumen biotae extract, 0.9-1.1 parts by weight of angelica sinensis extract, 0.9-1.1 parts by weight of eclipta prostrata extract, 0.9-1.1 parts by weight of mulberry leaf extract, 0.9-1.1 parts by weight of ganoderma atrum extract, 0.9-1.1 parts by weight of polygonum multiflorum extract, 0.9-1.1 parts by weight of dicranopteris pedata extract and 0.9-1.1 parts by weight of pine leaf fern extract; or (b) 0.9-1.1 parts by weight of a ginseng extract, 0.9-1.1 parts by weight of a cacumen biotae extract, 0.9-1.1 parts by weight of an angelica sinensis extract, 0.9-1.1 parts by weight of an eclipta prostrata extract, 0.9-1.1 parts by weight of a folium mori extract, 0.9-1.1 parts by weight of a ganoderma atrum extract, 0.9-1.1 parts by weight of a polygonum multiflorum extract, 0.9-1.1 parts by weight of a Chinese cinquefoil root extract and The preparation method of the extract comprises the following steps: crushing the raw materials, adding water for reflux, filtering and concentrating. Furthermore, 1.0 part by weight of each extract can be taken and uniformly mixed, 7.2 parts by weight of 75% ethanol is added, stirring and filtering are performed at room temperature, and the filtrate is concentrated to obtain the traditional Chinese medicine composition.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of medicine, in particular to a plant composition for activating hair follicles to promote hair growth and a preparation method thereof. BACKGROUND

[0003] Hair growth depends on the complex skin appendage structure of hair follicle, which periodically experiences anagen, catagen and telogen, and a healthy hair follicle can maintain normal hair cycle. However, when the hair follicle is disturbed by factors such as genetics, endocrine disorders, oxidative stress, chronic inflammation or insufficient nutrition, its function will be impaired, resulting in phenomena such as shortened anagen, prolonged telogen or miniaturization of hair follicle, ultimately leading to hair loss, sparseness or premature whitening of hair color. Therefore, activating hair follicle stem cells, regulating hair follicle cycle, improving scalp blood circulation and nutrition supply, and delaying hair follicle aging are the research focus in the field of hair regeneration and protection.

[0004] Market-wise, the global hair care product market has exceeded $10 billion, and is expected to continue to grow at a medium-high speed in the coming years. Consumers' attention to hair growth, hair growth, hair darkening and hair care products continues to increase, especially under the driving of hormone-free, natural ingredients, mild and efficient demands, functional raw materials of natural plant origin are increasingly favored by the market and research institutions. Compared with the side effects and long-term dependence of western medicine hair growth products (such as minoxidil and finasteride), natural plant medicines have become an important direction for the development of hair growth and hair care products due to their wide sources, high safety and mild and long-lasting effects.

[0005] Among numerous traditional Chinese medicinal materials, ginseng and radix angelicae sinensis are widely used in various traditional Chinese medicine compounds and external hair care preparations due to their excellent hair care, hair darkening and hair growth effects shown in traditional Chinese medicine books and modern pharmacological studies.

[0006] Ginseng is a plant of the Araliaceae family, which has the effects of tonifying qi and nourishing blood, and strengthening the body resistance. According to the Compendium of Materia Medica, it can calm the spirit, stabilize the soul, stop palpitation, eliminate evil qi, brighten the eyes and open the heart and benefit the intelligence, and is used in traditional Chinese medicine to treat hair loss caused by kidney deficiency, blood deficiency and deficiency of both qi and blood. Modern studies have shown that ginseng contains various active ingredients such as ginsenosides (such as Rg1, Rb1, Re, etc.), polysaccharides, volatile oils and trace elements, which have the biological effects of promoting hair papilla cell proliferation, enhancing hair follicle microcirculation and delaying hair follicle aging. Among them, ginsenoside Rg1 has been proven to regulate hair follicle stem cell proliferation and differentiation, and has a significant activating effect on hair follicles.

[0007] Radix Polygoni Multiflori is one of the traditional hair blackening herbs, and its root has a long history of medicinal use. It is listed as a top-grade medicinal material in Shennong's Herbal Classic and is believed to tonify the liver and kidney, nourish essence and blood, blacken the hair, and strengthen the bones and muscles. It is suitable for symptoms such as kidney deficiency, blood deficiency, and hair loss. Modern studies have found that stilbenes (such as THSG, i.e., 2,3,5,4'-tetrahydroxystilbene-2-O-glucoside) in Radix Polygoni Multiflori, anthraquinones (such as emodin and chrysophanol), and phospholipids have various effects such as antioxidant, anti-inflammatory, and promoting hair follicle cell growth, can delay hair follicle aging, improve hair papilla cell function, and thus play a role in hair growth and hair blackening. In addition, THSG also has free radical scavenging ability and can alleviate the damage of oxidative stress to hair follicles, and is one of the natural hair growth active molecules currently being actively studied.

[0008] Although ginseng and Radix Polygoni Multiflori have good hair growth potential, how to improve their biological activity through compatibility regulation, carrier optimization, emulsification technology or transdermal technology to achieve synergistic promotion of hair follicle activation and hair regeneration is a key difficulty and technical research direction in the field.

[0009] In summary, the development of a new hair growth external preparation based on natural active ingredients such as ginseng and Radix Polygoni Multiflori, using scientific compatibility and formulation process optimization, not only meets the current consumer demand for natural, safe, and effective personalized products, but also has good industrialization prospects and market potential. SUMMARY

[0010] The present application provides a composition comprising: (a) ginseng, Platycladus orientalis, Angelica sinensis, Eclipta prostrata, Morus alba, Ganoderma atrum, Radix Polygoni Multiflori, Dicranopteris dichotoma, and Matteuccia struthiopteris, each in an amount of 0.9-1.1 parts by weight; or (b) ginseng, Platycladus orientalis, Angelica sinensis, Eclipta prostrata, Morus alba, Ganoderma atrum, Radix Polygoni Multiflori, Disporum smilaceifolium, and Isoetes sinensis, each in an amount of 0.9-1.1 parts by weight.

[0011] In some embodiments of the present application, the composition comprises: ginseng, Platycladus orientalis, Angelica sinensis, Eclipta prostrata, Morus alba, Ganoderma atrum, Radix Polygoni Multiflori, Dicranopteris dichotoma, and Matteuccia struthiopteris, each in an amount of 0.9-1.1 parts by weight.

[0012] In some embodiments of the present application, the composition comprises: ginseng, Platycladus orientalis, Angelica sinensis, Eclipta prostrata, Morus alba, Ganoderma atrum, Radix Polygoni Multiflori, Disporum smilaceifolium, and Isoetes sinensis, each in an amount of 0.9-1.1 parts by weight.

[0013] In some embodiments of the present application, the composition comprises: (a) equal parts by weight of ginseng, Platycladus orientalis, Angelica sinensis, Eclipta prostrata, Morus alba, Ganoderma atrum, Radix Polygoni Multiflori, Dicranopteris dichotoma, and Matteuccia struthiopteris; or (b) equal weight parts of ginseng, biota leaf, angelica, rhizoma zedoariae, mulberry leaf, black ganoderma, radix falcaliae, radix paeoniae alba, and clematis.

[0014] In some embodiments of the present application, the composition comprises: equal weight parts of ginseng, biota leaf, angelica, rhizoma zedoariae, mulberry leaf, black ganoderma, radix falcaliae, and clematis.

[0015] In some embodiments of the present application, the composition comprises: equal weight parts of ginseng, biota leaf, angelica, rhizoma zedoariae, mulberry leaf, black ganoderma, radix falcaliae, and clematis.

[0016] The present application provides a composition comprising: (a) ginseng extract, biota leaf extract, angelica extract, rhizoma zedoariae extract, mulberry leaf extract, black ganoderma extract, radix falcaliae extract, and clematis extract, each in a weight part of 0.9-1.1; or (b) ginseng extract, biota leaf extract, angelica extract, rhizoma zedoariae extract, mulberry leaf extract, black ganoderma extract, radix falcaliae extract, and clematis extract, each in a weight part of 0.9-1.1; The preparation method of the extract is: grinding each raw material, adding water to reflux, filtering and concentrating to obtain.

[0017] The present application provides a composition comprising: (a) equal weight parts of ginseng extract, biota leaf extract, angelica extract, rhizoma zedoariae extract, mulberry leaf extract, black ganoderma extract, radix falcaliae extract, and clematis extract; or (b) equal weight parts of ginseng extract, biota leaf extract, angelica extract, rhizoma zedoariae extract, mulberry leaf extract, black ganoderma extract, radix falcaliae extract, and clematis extract; The preparation method of the extract is: grinding each raw material, adding water to reflux, filtering and concentrating to obtain.

[0018] In some embodiments of the present application, the preparation method of the extract is: using a grinder to grind each plant raw material to an average particle size of about 200 microns, taking 300 grams, adding 3 kilograms of water, and stirring at 100 revolutions per minute to reflux at 100°C for 5 hours, and then naturally cooling to room temperature; filtering to obtain a clear solution, concentrating to 600 milliliters under reduced pressure at 30°C, and freeze-drying at -20°C to obtain a powdered extract of each plant raw material.

[0019] In some embodiments of the present application, the preparation method of the mulberry leaf extract is as follows: taking mulberry leaves as raw material, after cleaning and drying, the mulberry leaves are put into a jar, 5 times of water by weight is used as solvent, heated to reflux for 3 times, each time for 2 hours, the 3 times of extraction liquid is combined, the water is concentrated and evaporated to the extract with specific gravity d=1.20, the extract is left to settle, centrifuged, the solution is spray dried, crushed, and sieved, and then the mulberry leaf extract is obtained.

[0020] The present application provides a composition comprising: equal weight parts of ginseng extract, cypress leaf extract, angelica extract, rhizoma copidis extract, mulberry leaf extract, black ganoderma extract, radix falconis extract, false mao chi extract, and flat branch tree fern extract. The preparation method of the extract is as follows: using a pulverizer to crush each plant raw material to an average particle size of about 200 microns, taking 300 grams, adding 3 kilograms of water, stirring at 100 revolutions per minute, boiling to 100℃ reflux for 5 hours, and naturally cooling to room temperature; filtering to obtain a clear solution, concentrating to 600 milliliters under reduced pressure at 30℃, and freeze-drying at-20℃ to obtain a powdered extract of each plant raw material.

[0021] The present application provides a composition comprising: equal weight parts of ginseng extract, cypress leaf extract, angelica extract, rhizoma copidis extract, mulberry leaf extract, black ganoderma extract, radix falconis extract, Chinese li white extract, and pen tube grass extract. The preparation method of the extract is as follows: using a pulverizer to crush each plant raw material to an average particle size of about 200 microns, taking 300 grams, adding 3 kilograms of water, stirring at 100 revolutions per minute, boiling to 100℃ reflux for 5 hours, and naturally cooling to room temperature; filtering to obtain a clear solution, concentrating to 600 milliliters under reduced pressure at 30℃, and freeze-drying at-20℃ to obtain a powdered extract of each plant raw material.

[0022] The present application provides a composition, and the preparation method of the composition is as follows: (a) taking ginseng, cypress leaf, angelica, rhizoma copidis, mulberry leaf, black ganoderma, radix falconis, false mao chi, and flat branch tree fern; or, taking ginseng, cypress leaf, angelica, rhizoma copidis, mulberry leaf, black ganoderma, radix falconis, Chinese li white, and pen tube grass; (b) crushing each plant raw material, adding water to reflux, filtering after cooling, and concentrating to obtain a powdered extract of each plant raw material; (c) taking 0.9-1.1 weight parts of each extract, mixing uniformly, adding 7-8 weight parts of 75% ethanol, stirring at room temperature, filtering, and concentrating the filtrate to obtain the composition.

[0023] In some embodiments of the present application, the ginseng is dried rootstock. In some embodiments of the present application, the biota leaf is dried leaf. In some embodiments of the present application, the angelica is dried root. In some embodiments of the present application, the rhizoma zedoariae is dried whole herb. In some embodiments of the present application, the mulberry leaf is dried leaf. In some embodiments of the present application, the black ganoderma is dried fruiting body. In some embodiments of the present application, the radix polygoni multiflori is dried tuber. In some embodiments of the present application, the dicranopteris is dried leaf. In some embodiments of the present application, the plagiogyria is dried aboveground part. In some embodiments of the present application, the liriope is dried leaf. In some embodiments of the present application, the stylophyllum is dried whole herb. In some embodiments of the present application, the aspidium is dried whole herb. In some embodiments of the present application, the dryopteris is dried root.

[0024] In some embodiments of the present application, the preparation method of the composition is: (a) taking ginseng, biota leaf, angelica, rhizoma zedoariae, mulberry leaf, black ganoderma, radix polygoni multiflori, dicranopteris, plagiogyria; or, taking ginseng, biota leaf, angelica, rhizoma zedoariae, mulberry leaf, black ganoderma, radix polygoni multiflori, liriope, stylophyllum; (b) respectively crushing, adding water to reflux, filtering after cooling, and concentrating to obtain powdered extract of each plant raw material; (c) taking 1.0 parts by weight of each extract, mixing uniformly, adding 7.2 parts by weight of 75% ethanol, stirring at room temperature, filtering, and concentrating the filtrate.

[0025] In some embodiments of the present application, the preparation method of the composition is: (a) taking ginseng, biota leaf, angelica, rhizoma zedoariae, mulberry leaf, black ganoderma, radix polygoni multiflori, dicranopteris, plagiogyria; (b) respectively crushing, adding water to reflux, filtering after cooling, and concentrating to obtain powdered extract of each plant raw material; (c) taking 1.0 parts by weight of each extract, mixing uniformly, adding 7.2 parts by weight of 75% ethanol, stirring at room temperature, filtering, and concentrating the filtrate.

[0026] In some embodiments of the present application, the preparation method of the composition is: (a) taking ginseng, biota leaf, angelica, rhizoma zedoariae, mulberry leaf, black ganoderma, radix polygoni multiflori, liriope, stylophyllum; (b) respectively crushing, adding water to reflux, filtering after cooling, and concentrating to obtain powdered extract of each plant raw material; (c) taking 1.0 parts by weight of each extract, mixing uniformly, adding 7.2 parts by weight of 75% ethanol, stirring at room temperature, filtering, and concentrating the filtrate.

[0027] In some embodiments of the present application, the water refluxing in (b) is carried out by adding 10 times the weight of water to each plant material. In some embodiments of the present application, the water refluxing in (b) is carried out for 5 hours.

[0028] In some embodiments, the concentration in (c) is freeze-drying. In some embodiments, the concentration in (c) is freeze-drying after concentration under reduced pressure at 30°C. In some embodiments, the freeze-drying temperature is -20°C.

[0029] A composition, the preparation method of which is: (a) taking plant materials of ginseng, biota leaf, angelica, rhizoma typhonii, mulberry leaf, black ganoderma, radix falcaliae, scytophorus and pyrrosia petiolosa; (b) grinding each plant material in (a) separately, refluxing each plant material with 10 times the weight of water for 5 hours, filtering after cooling, and concentrating to obtain a powdered extract of each plant material; (c) taking 1.0 parts by weight of each extract in (b), mixing uniformly, adding 7.2 parts by weight of 75% ethanol, stirring at room temperature, filtering, concentrating the filtrate under reduced pressure at 30°C to 1 / 3 of the volume, and freeze-drying at -20°C, to obtain the composition.

[0030] A composition, the preparation method of which is: (a) taking plant materials of ginseng, biota leaf, angelica, rhizoma typhonii, mulberry leaf, black ganoderma, radix falcaliae, scytophorus and pyrrosia petiolosa; (b) grinding each plant material in (a) separately, refluxing each plant material with 10 times the weight of water for 5 hours, filtering after cooling, and concentrating to obtain a powdered extract of each plant material; (c) taking 1.0 parts by weight of each extract in (b), mixing uniformly, adding 7.2 parts by weight of 75% ethanol, stirring at room temperature, filtering, concentrating the filtrate under reduced pressure at 30°C to 1 / 3 of the volume, and freeze-drying at -20°C, to obtain the composition.

[0031] The present application provides a composition, the preparation method of which is as described in Preparation Example 1. The present application provides a composition, the preparation method of which is as described in Preparation Example 2.

[0032] The present application provides a preparation method of a composition, the preparation method being: (a) taking ginseng, biota leaf, angelica, rhizoma typhonii, mulberry leaf, black ganoderma, radix falcaliae, scytophorus, pyrrosia petiolosa; or, taking ginseng, biota leaf, angelica, rhizoma typhonii, mulberry leaf, black ganoderma, radix falcaliae, scytophorus, pyrrosia petiolosa; (b) each of the plant raw materials is ground, refluxed with water, filtered after cooling, and concentrated to obtain a powdered extract of each plant raw material; (c) 0.9-1.1 parts by weight of each extract is taken, mixed uniformly, 7-8 parts by weight of 75% ethanol is added, stirred at room temperature, filtered, and the filtrate is concentrated.

[0033] The present application provides a preparation method of a composition, which is as described in Preparation Example 1. (a) plant raw materials of ginseng, Chinese arborvitae leaves, angelica, Chinese knotweed, mulberry leaves, black ganoderma, radix falcaliae, false mao chi, and flat-branch pine leaf fern are taken; (b) each of the plant raw materials is ground, refluxed with water, filtered after cooling, and concentrated to obtain a powdered extract of each plant raw material; (c) 0.9-1.1 parts by weight of each extract is taken, mixed uniformly, 7-8 parts by weight of 75% ethanol is added, stirred at room temperature, filtered, and the filtrate is concentrated.

[0034] The present application provides a preparation method of a composition, which is as described in Preparation Example 1. (a) plant raw materials of ginseng, Chinese arborvitae leaves, angelica, Chinese knotweed, mulberry leaves, black ganoderma, radix falcaliae, false mao chi, and flat-branch pine leaf fern are taken; (b) each of the plant raw materials is ground, refluxed with water, filtered after cooling, and concentrated to obtain a powdered extract of each plant raw material; (c) 0.9-1.1 parts by weight of each extract is taken, mixed uniformly, 7-8 parts by weight of 75% ethanol is added, stirred at room temperature, filtered, and the filtrate is concentrated.

[0035] The present application provides a preparation method of a composition, which is as described in Preparation Example 1. The present application provides a preparation method of a composition, which is as described in Preparation Example 2.

[0036] The present application provides a hair-growing external liquid preparation, which comprises any one of the compositions of the present application.

[0037] The present application provides a hair-growing external liquid preparation, which comprises a composition, the preparation method of which is as follows: (a) plant raw materials of ginseng, Chinese arborvitae leaves, angelica, Chinese knotweed, mulberry leaves, black ganoderma, radix falcaliae, false mao chi, and flat-branch pine leaf fern are taken; or, plant raw materials of ginseng, Chinese arborvitae leaves, angelica, Chinese knotweed, mulberry leaves, black ganoderma, radix falcaliae, Chinese white, and Chinese pipevine are taken; (b) each of the plant raw materials is ground, refluxed with water, filtered after cooling, and concentrated to obtain a powdered extract of each plant raw material; (c) 0.9-1.1 parts by weight of each extract is taken, mixed uniformly, 7-8 parts by weight of 75% ethanol is added, stirred at room temperature, filtered, and the filtrate is concentrated.

[0038] The present application provides a hair growth external liquid preparation, which comprises a composition, and a preparation method of the composition is as follows: (a) taking plant raw materials of ginseng, biota leaf, angelica, rhizoma polygoni avicularis, mulberry leaf, black ganoderma, radix falcaliae, false mao chi, pteridium revolutum; (b) respectively crushing, adding water to reflux, filtering after cooling, and concentrating to obtain powdered extracts of each plant raw material; (c) taking 0.9-1.1 parts by weight of each extract, mixing uniformly, adding 7-8 parts by weight of 75% ethanol, stirring at room temperature, filtering, and concentrating the filtrate to obtain.

[0039] The present application provides a medicine, which comprises any composition of the present application.

[0040] The present application provides a pharmaceutical preparation, which comprises any composition of the present application.

[0041] The present application provides use of the composition as described in the present application in the preparation of a medicine for promoting hair growth.

[0042] In some embodiments of the present application, the promoting hair growth is increasing hair length. In some embodiments of the present application, the promoting hair growth is activating hair follicles. In some embodiments of the present application, the promoting hair growth is increasing the number of hair follicles.

[0043] The present application provides use of the hair growth external liquid preparation as described in the present application in the preparation of a medicine for promoting hair growth.

[0044] The present application provides use of the composition as described in the present application in the preparation of a medicine for treating androgenetic alopecia.

[0045] The present application provides use of the hair growth external liquid preparation as described in the present application in the preparation of a medicine for treating androgenetic alopecia. DETAILED DESCRIPTION

[0046] The present application is further illustrated by the following examples, but the scope of the patent protection is not limited in the range of the examples. The experimental methods in the following examples are not specified, which are selected according to the conventional methods and conditions, or according to the instructions of the commercial products. The reagents and raw materials used in the present disclosure can be obtained from the market.

[0047] The raw materials used in the following examples include: ginseng (dried rhizome), biota leaf (dried leaf), angelica (dried root), rhizoma polygoni avicularis (dried whole plant), mulberry leaf (dried leaf), black ganoderma (dried fruiting body), radix falcaliae (dried root), false mao chi (dried leaf), pteridium revolutum (dried aerial part), Chinese li white (dried leaf), pen tube grass (dried whole plant), iron angle fern (dried whole plant), drynaria (dried root).

[0048] Preparation Example 1: Ginseng, Platycladus orientalis, Angelica sinensis, Argy- tis filixifolia, Morus alba, Ganoderma atrum, Polygonum multiflorum, Dicranopteris dichotoma, Oleandra undulata were washed with water, and then dried. Each plant material was ground to an average particle size of about 200 microns using a grinder. 300 grams of each plant material was added to 3 kilograms of water, and boiled to 100°C under stirring at 100 rpm for 5 hours. The mixture was naturally cooled to room temperature. The clear solution was obtained by filtration. The solution was concentrated to 600 milliliters under reduced pressure at 30°C, and then freeze-dried at -20°C to obtain a powder extract of each plant material (i.e., ginseng extract, Platycladus orientalis extract, Angelica sinensis extract, Argy- tis filixifolia extract, Morus alba extract, Ganoderma atrum extract, Polygonum multiflorum extract, Dicranopteris dichotoma extract, and Oleandra undulata extract). 10 grams of each extract was mixed to obtain a mixture, which was added to a stirring tank. 72 grams of 75% ethanol aqueous solution (v / v) was added to the mixture, and stirred at room temperature for 2.5 hours at 100 rpm. The mixture was allowed to stand for 24 hours. The clear solution was obtained by filtration. The solution was concentrated to 1 / 3 of the volume under reduced pressure at 30°C, and then freeze-dried at -20°C to obtain a powder composition 1.

[0049] Preparation Example 2: Ginseng, Platycladus orientalis, Angelica sinensis, Argy- tis filixifolia, Morus alba, Ganoderma atrum, Polygonum multiflorum, Dendropanax chinensis, and Rhizoma Phrymarthi were washed with water, and then dried. Each plant material was ground to an average particle size of about 200 microns using a grinder. 300 grams of each plant material was added to 3 kilograms of water, and boiled to 100°C under stirring at 100 rpm for 5 hours. The mixture was naturally cooled to room temperature. The clear solution was obtained by filtration. The solution was concentrated to 600 milliliters under reduced pressure at 30°C, and then freeze-dried at -20°C to obtain a powder extract of each plant material (i.e., ginseng extract, Platycladus orientalis extract, Angelica sinensis extract, Argy- tis filixifolia extract, Morus alba extract, Ganoderma atrum extract, Polygonum multiflorum extract, Dendropanax chinensis extract, and Rhizoma Phrymarthi extract). 10 grams of each extract was mixed to obtain a mixture, which was added to a stirring tank. 72 grams of 75% ethanol aqueous solution (v / v) was added to the mixture, and stirred at room temperature for 2.5 hours at 100 rpm. The mixture was allowed to stand for 24 hours. The clear solution was obtained by filtration. The solution was concentrated to 1 / 3 of the volume under reduced pressure at 30°C, and then freeze-dried at -20°C to obtain a powder composition 2.

[0050] Preparation Example 3: Ginseng, Platycladus orientalis, Angelica sinensis, Argy- tis filixifolia, Morus alba, Ganoderma atrum, Polygonum multiflorum, Asplenium rynchobactron, Dryo-phyllum hirtipes were washed with water, and dried. Each plant material was ground to an average particle size of about 200 microns using a grinder. 300 grams of each plant material was added to 3 liters of water, and boiled to 100°C for 5 hours with stirring at 100 rpm. The mixture was allowed to cool to room temperature. The mixture was filtered to obtain a clear solution. The solution was concentrated to 600 ml under reduced pressure at 30°C, and freeze-dried at -20°C to obtain a powder extract of each plant material (i.e., ginseng extract, Platycladus orientalis extract, Angelica sinensis extract, Argytis filixifolia extract, Morus alba extract, Ganoderma atrum extract, Polygonum multiflorum extract, Asplenium rynchobactron extract, Dryophyllum hirtipes extract). 10 grams of each extract was mixed together, and placed in a stirring tank. 72 grams of 75% ethanol (v / v) was added, and stirred at room temperature for 2.5 hours at 100 rpm. The mixture was allowed to stand for 24 hours. The mixture was filtered to obtain a clear solution. The solution was concentrated to 1 / 3 of the original volume under reduced pressure at 30°C, and freeze-dried at -20°C to obtain powder composition 3.

[0051] Preparation Example 4: Ginseng, Platycladus orientalis, Angelica sinensis, Argy- tis filixifolia, Morus alba, Ganoderma atrum, Polygonum multiflorum, Dicranopteris dichotoma, Dryopteris campylopetala were washed with water, and dried. Each plant material was ground to an average particle size of about 200 microns using a grinder. 300 grams of each plant material was added to 3 liters of water, and boiled to 100°C for 5 hours with stirring at 100 rpm. The mixture was allowed to cool to room temperature. The mixture was filtered to obtain a clear solution. The solution was concentrated to 600 ml under reduced pressure at 30°C, and freeze-dried at -20°C to obtain a powder extract of each plant material. 10 grams of ginseng extract, Platycladus orientalis extract, Angelica sinensis extract, Argytis filixifolia extract, Morus alba extract, Ganoderma atrum extract, Polygonum multiflorum extract was mixed together, and 1 gram of Dicranopteris dichotoma extract, and 19 grams of Dryopteris campylopetala extract was added. The mixture was placed in a stirring tank. 72 grams of 75% ethanol (v / v) was added, and stirred at room temperature for 2.5 hours at 100 rpm. The mixture was allowed to stand for 24 hours. The mixture was filtered to obtain a clear solution. The solution was concentrated to 1 / 3 of the original volume under reduced pressure at 30°C, and freeze-dried at -20°C to obtain powder composition 4.

[0052] Preparation Example 5: Ginseng, Platycladus orientalis, Angelica sinensis, Eclipta prostrata, Morus alba, Ganoderma atrum, Polygonum multiflorum, Dicranopteris dichotoma, and Matteuccia strutio were washed with water, and then dried. Each plant material was ground to an average particle size of about 200 microns using a grinder. 300 grams of each plant material was added to 3 kg of water, and boiled to 100°C for 5 hours with stirring at 100 rpm. The mixture was then naturally cooled to room temperature. The clear solution was filtered, and concentrated to 600 ml under reduced pressure at 30°C. The solution was then freeze-dried at -20°C to obtain a powder extract of each plant material. 10 grams of each of the ginseng extract, Platycladus orientalis extract, Angelica sinensis extract, Eclipta prostrata extract, Morus alba extract, Ganoderma atrum extract, and Polygonum multiflorum extract was mixed with 19 grams of the Dicranopteris dichotoma extract and 1 gram of the Matteuccia strutio extract. The mixture was then added to a stirring tank, and 72 grams of 75% ethanol water solution (v / v) was added. The mixture was stirred at room temperature for 2.5 hours at 100 rpm, and then left to stand for 24 hours. The clear solution was filtered, and concentrated to 1 / 3 of the volume under reduced pressure at 30°C. The solution was then freeze-dried at -20°C to obtain a powder composition 5.

[0053] Preparation Example 6: Each extract was prepared according to the method described in paragraphs

[0154] to

[0173] of the specification of patent publication CN119818565A. Then, 72 parts by weight of the Platycladus orientalis extract, 16 parts by weight of the Polygonum multiflorum extract, 75 parts by weight of the Angelica sinensis extract, 46 parts by weight of the Morus alba extract, 37 parts by weight of the Eclipta prostrata extract, 23 parts by weight of the ginseng extract, and 55 parts by weight of the Salvia miltiorrhiza extract were mixed. 900 parts by weight of water was then added, and the mixture was stirred to dissolve as much as possible. The filtrate was then freeze-dried at -20°C to obtain a powder composition 6.

[0054] Preparation Example 7: Each extract was prepared according to the method described in paragraph

[0013] of the specification of patent publication CN107625924A. Preparation Example 7: Each extract was prepared according to the method described in paragraph

[0013] of the specification of patent publication CN107625924A.

[0055] Preparation Example 8: Prepared according to the method described in paragraphs

[0024] to

[0028] of the specification of patent publication CN111388363A. Take 18 parts of Radix Polygoni Multiflori, 16 parts of Angelica sinensis, 15 parts of mulberry, 15 parts of Gleditsia sinensis, 12 parts of Polygonatum sibiricum, 8 parts of Eclipta prostrata (Eclipta prostrata is the dried aerial part of Eclipta prostrata), 6 parts of Ganoderma lucidum, 22 parts of Camellia sinensis, 16 parts of Biota orientalis, 8 parts of Taraxacum mongolicum, 8 parts of Ligustrum lucidum, 6 parts of Zingiber officinale, 6 parts of Panax ginseng, 6 parts of ginger, mix, crush to an average particle size of about 200 microns, mix evenly, and obtain powder composition 8.

[0056] Preparation Example 9: Take 300 grams of composition 8 prepared according to the method described in Preparation Example 8, add 3 kilograms of water, and boil to 100°C reflux for 5 hours under stirring at 100 rpm. Naturally cool to room temperature; filter to obtain a clear solution, concentrate to 600 milliliters under reduced pressure at 30°C, and freeze-dry at -20°C to obtain an extract. Take 90 grams of the extract, put it into a stirring tank, add 72 grams of 75% ethanol aqueous solution (v / v), stir at room temperature (100 rpm) for 2.5 hours, and then stand for 24 hours. Filter to obtain a clear solution, concentrate to 1 / 3 volume under reduced pressure at 30°C, and freeze-dry at -20°C to obtain powder composition 9.

[0057] Preparation Example 10: Prepared according to the method described in paragraphs

[0069] to

[0074] of the specification of patent publication CN111700841A.

[0058] Take black ganoderma, ginseng root, chuanxiong, radix polygoni multiflori, angelica sinensis, soapberry peel, soap pod, camellia seed, black mulberry, cypress leaf, ginger root, sophora flavescens, and flat stem astragalus. Use a pulverizer to crush each plant raw material to an average particle size of about 200 microns. Take 300 grams, add 3 kilograms of water, and boil to 100°C reflux for 5 hours under stirring at 100 rpm. Naturally cool to room temperature; filter to obtain a clear solution, concentrate to 600 milliliters under reduced pressure at 30°C, and freeze-dry at -20°C to obtain a powder extract of each plant raw material. Then take 8 parts of black ganoderma extract, 0.6 parts of ginseng root extract, 1.3 parts of chuanxiong extract, 1 part of radix polygoni multiflori extract, 1.5 parts of angelica sinensis extract, 0.6 parts of soapberry peel extract, 2 parts of soap pod extract, 2.5 parts of camellia seed extract, 3 parts of black mulberry extract, 0.7 parts of cypress leaf extract, 3 parts of ginger root extract, 2 parts of sophora flavescens extract, and 1.3 parts of flat stem astragalus extract, mix evenly, and obtain powder composition 10.

[0059] Preparation Example 11: Prepare powder composition 11 according to the method described in paragraphs

[0019] to

[0039] of the specification of patent publication CN119454888A.

[0060] Preparation Example 12: Prepared according to the method described in paragraphs

[0052] to

[0061] of the specification of patent publication CN113509539A. The specific steps are as follows.

[0061] Take He Shou Wu 6g, Ginseng 9g, Ganoderma 5g, Dang Gui 8g, Sanqi 3g, Chuanqiong 4g, Black Soybean 15g, Black Wolfberry 10g, Black Sesame 12g, Black Mulberry 12g, Tougucao 6g, Mulberry Leaf 9g, Baiji 6g, Ruzheng 8g, Lotus Leaf 6g, Ginger 20g, Jujube 6g, Rose Flower 5g, Goutengzi 5g, Buguzhi 5g, Qingdai 5g, Chrysanthemum 5g, Laifeng 5g, Bai Zhi 6g, Shedanzi 6g, Digengzi 6g, Cyparissus 30g, Soap Nut 30g, Bai Zi 5g, Hutianma 6g, Huangqi 6g, Dangshen 6g, Duzhong 6g, Hanmoxian 6g, Niuqi 6g, quickly rinsed with water to get clean mixed medicinal materials; Put the clean mixed medicinal materials into the medicine pot, add normal temperature water, 3cm higher than the mixed medicinal materials, soak for 30min, the mixed medicinal materials swell after absorbing water, add water again to 3cm higher than the mixed medicinal materials, after boiling with high fire, change to low fire and decoct for 30min, cool and filter out medicinal liquid A for standby; Add hot water to the medicine residue in the medicine pot for secondary decoction, the liquid level of hot water is 1cm higher than the medicine residue, decoct with low fire for 20min, cool and filter out medicinal liquid B; Mix medicinal liquid A and medicinal liquid B, then decoct and concentrate with low fire, then freeze dry at-20℃ to get powder-like composition 12.

[0062] Preparation Example 13: Prepared according to the method described in paragraph

[0044] of the specification of patent publication CN108653707A. The specific steps are as follows.

[0063] Take He Shou Wu 25 parts, Black Wolfberry 24 parts, Sangbaipi 10 parts, Gallnut 12 parts, Qingxianzi 18 parts, Hixiancao 8 parts, Kudingcha 12 parts, Cistanche 25 parts, Eupatorium Fortunei 22 parts, Hanliancao 25 parts, Cyparissus 12 parts, Lulutong 20 parts, Shedanzi 15 parts, Huixiancai 12 parts, Mactong 12 parts, Rosmarinus officinalis 12 parts, Xiangmaocao 12 parts, Lavender 12 parts, Ginseng 12 parts, Danshen 12 parts, Chuanqiong 12 parts, Danggui 12 parts, Bai Zhi 12 parts, Soap Nut 12 parts, Mulberry Leaf 8 parts, Pine Needle 25 parts, Ginger 8 parts, Mugwort 25 parts, Ganoderma 12 parts; Mix evenly, use a pulverizer to crush to an average particle size of about 200 microns to get mixed powder; Add 5 times the weight of water to the mixed powder and heat to 80℃ for 2 hours to get water extract, filter to get filtrate, concentrate the filtrate, then freeze dry to get powder-like composition 13.

[0064] Preparation Example 14: Prepared according to the method described in paragraphs

[0028] -

[0037] of the specification of patent publication CN107970322A. Concentrate the finished traditional Chinese medicine to a concentration of 4g / ml, freeze dry at-20℃ to get powder-like composition 14.

[0065] Preparation Example 15: Take Dicranopteris dichotoma, wood fern, respectively, washed with water, dry. Using a pulverizer each plant material is crushed to an average particle size of about 200 microns, 300 grams, add 3 kg of water, 100 revolutions per minute stirring boiled to 100 °C reflux 5 hours, natural cooling to room temperature; filtration, get clear solution, 30 °C under reduced pressure concentration to 600 milliliters, -20 °C freeze drying, get each plant material powder extract (ie Dicranopteris dichotoma extract, wood fern extract). Each take 20 grams of extract, mixed evenly, put into a stirred tank, add 32 grams of 75% ethanol aqueous solution (v / v), room temperature stirring (100 revolutions per minute) 2.5 hours, then stand for 24 hours. Filtration, get clear solution, 30 °C under reduced pressure concentration to 1 / 3 volume, -20 °C freeze drying, get powder 15.

[0066] Preparation Example 16: Take Chinese liu white, pen tube grass, respectively, washed with water, dry. Using a pulverizer each plant material is crushed to an average particle size of about 200 microns, 300 grams, add 3 kg of water, 100 revolutions per minute stirring boiled to 100 °C reflux 5 hours, natural cooling to room temperature; filtration, get clear solution, 30 °C under reduced pressure concentration to 600 milliliters, -20 °C freeze drying, get each plant material powder extract (ie Chinese liu white extract, pen tube grass extract). Each take 20 grams of extract, mixed evenly, put into a stirred tank, add 32 grams of 75% ethanol aqueous solution (v / v), room temperature stirring (100 revolutions per minute) 2.5 hours, then stand for 24 hours. Filtration, get clear solution, 30 °C under reduced pressure concentration to 1 / 3 volume, -20 °C freeze drying, get powder 16.

[0067] Preparation Example 17: Take Asplenium antiquum, Drynaria fortunei, respectively, washed with water, dry. Using a pulverizer each plant material is crushed to an average particle size of about 200 microns, 300 grams, add 3 kg of water, 100 revolutions per minute stirring boiled to 100 °C reflux 5 hours, natural cooling to room temperature; filtration, get clear solution, 30 °C under reduced pressure concentration to 600 milliliters, -20 °C freeze drying, get each plant material powder extract (ie Asplenium antiquum extract, Drynaria fortunei extract). Each take 20 grams of extract, mixed evenly, put into a stirred tank, add 32 grams of 75% ethanol aqueous solution (v / v), room temperature stirring (100 revolutions per minute) 2.5 hours, then stand for 24 hours. Filtration, get clear solution, 30 °C under reduced pressure concentration to 1 / 3 volume, -20 °C freeze drying, get powder 17.

[0068] Preparation Example 18: Ginseng, Biyang, Dangui, Euryale, Mulberry leaf, He Lingzhi, Huzhuwu were washed with water, and then dried. Each plant material was ground to an average particle size of about 200 microns using a grinder. 300 grams of each plant material was added to 3 kg of water, and boiled to 100°C for 5 hours with stirring at 100 rpm. The mixture was then naturally cooled to room temperature. The mixture was filtered to obtain a clear solution. The solution was concentrated to 600 ml under reduced pressure at 30°C, and then freeze-dried at -20°C to obtain a powder extract of each plant material (i.e., ginseng extract, biyang extract, dangui extract, euryale extract, mulberry leaf extract, he lingzhi extract, huzhuwu extract). 10 grams of each extract was mixed together, and then added to a stirring tank. 56 grams of 75% ethanol (v / v) was added to the tank, and the mixture was stirred at room temperature for 2.5 hours at 100 rpm. The mixture was then allowed to stand for 24 hours. The mixture was filtered to obtain a clear solution. The solution was concentrated to 1 / 3 of its volume under reduced pressure at 30°C, and then freeze-dried at -20°C to obtain a powder composition 18.

[0069] Example 1. Effect test Each of the compositions (composition 1 to composition 18) prepared according to the methods described in Preparation Examples 1 to 18 was prepared into a solution or a uniform suspension at a desired concentration using 70% ethanol (v / v), as shown in Table 1.

[0070] Eight-week-old male C57BL / 6J mice weighing 21.6 to 22.6 grams were randomly divided into groups of 10. A 1 cm x 1 cm area was selected on the left upper back of each mouse, and the area was shaved. A 70% ethanol (v / v) solution of testosterone was prepared, in which the concentration of testosterone was 5 mg / mL.

[0071] The mice in the model group were uniformly applied with 0.1 mL of the 70% ethanol solution of testosterone on the shaved area of the back of the mice from the first day after shaving, once a day for 10 consecutive days.

[0072] The mice in the blank group were not treated.

[0073] The mice in each composition group were uniformly applied with 0.1 mL of the 70% ethanol solution of testosterone on the shaved area of the back of the mice from the first day after shaving, and 3 hours later, 0.1 mL of the 70% ethanol (v / v) solution of each corresponding composition was uniformly applied on the shaved area of the back of the mice. The mice were applied with testosterone and the composition once a day for 10 consecutive days.

[0074] Each group of mice was normally fed and watered.

[0075] On day 11 after depilation, the mice were photographed and the skin of the depilated area was examined for hair growth. Six skin samples were randomly selected from the depilated area and the melanin content was measured using a skin color meter. Ten hairs were randomly selected from the depilated area of each mouse and the length of each hair was measured. The average length of the hairs was calculated. The average length of the hairs of the mice in each group is shown in Table 1.

[0076] Statistical analysis showed that on day 11 after depilation, the length of the hairs of the mice in the model group was significantly shorter than that of the mice in the blank group. The length of the hairs of the mice in the composition 1 group (100 mg / mL), the composition 1 group (50 mg / mL), the composition 2 group (100 mg / mL) and the composition 2 group (50 mg / mL) was significantly longer than that of the mice in the model group and the mice in the other composition groups (P < 0.05). The length of the hairs of the mice in the composition 18 group was significantly longer than that of the mice in the model group (P < 0.05).

[0077] The results show that the hair growth promoting activity of the composition 1 and the composition 2 is significantly stronger than that of the other compositions, including the compositions prepared according to the prior art. The hair growth promoting activity of the composition 1 and the composition 2 is even stronger than that of the composition 3 to the composition 18 at a lower dosage. The composition 1 is stronger than the composition 15 and the composition 18, the composition 2 is stronger than the composition 16 and the composition 18, and the composition 1 and the composition 2 are both stronger than the composition 3, indicating the existence of synergistic effect. However, when the Dryopteris laeta and the Woodsia alpina in the composition 1 and the composition 2 are replaced by the Dicranopteris dichotoma and the Phymatopteris subfurfuracea, respectively, the activity is greatly reduced. The activity of the composition 4 and the composition 5, which are obtained by changing the ratio of the Dicranopteris dichotoma and the Phymatopteris subfurfuracea, is also greatly reduced.

[0078] Table 1. Hair length On day 11 after depilation, the melanin index of the depilated area of the back of the mice was also measured using a skin melanin analyzer (Mexameter® MX 18). The larger the value, the higher the melanin content. The relative value of the melanin index of the back of the mice in each group compared to the blank group was calculated (the melanin index of the mice in each group divided by the melanin index of the mice in the blank group). The data in Table 2 are the average values of the mice in each group. Statistical analysis showed that the relative value of the melanin index of the back of the mice in the composition 1 group (100 mg / mL), the composition 1 group (50 mg / mL), the composition 2 group (100 mg / mL) and the composition 2 group (50 mg / mL) was significantly higher than that of the mice in the model group and the mice in the other composition groups (P < 0.05).

[0079] Table 2. Relative melanin content of the depilated area on day 10 after depilation Without being bound by theory, the synthesis of melanin in the skin of mice is closely related to the growth condition of hair follicles. Hair follicles in the growth phase stimulate melanocytes in the basal layer of the epidermis to be more active, which leads to darkening of the skin, which is visible to the naked eye as "darkening". The increase in pigmentation after treatment with the specific composition indicates that the hair follicles are activated and enter the growth phase.

[0080] On the 11th day after depilation, the newly grown hair in the depilation area of the model group animals was obviously sparse, and the hair was thin; the newly grown hair in the depilation area of the animals in the composition 1 group and the composition 2 group was the most dense, the darkest, the longest and the thickest.

[0081] The mice were then sacrificed, and the skin of the modeling site of the mice on the 11th day after depilation was taken, fixed in a 4% formaldehyde solution, dehydrated, paraffin-embedded, sectioned, HE-stained, and then the section images were collected by a digital slice scanner and analyzed, the number of hair follicles per unit area was counted, and the average value of the animals in each group was calculated.

[0082] As shown in Table 3, the data in the table are the average number of hair follicles per unit area (150 square microns) of the animals in each group. The statistical analysis results show that the number of hair follicles per unit area in the depilation area (modeling area) of the back of the animals in the composition 1 group (100 mg / mL), the composition 1 group (50 mg / mL), the composition 2 group (100 mg / mL), and the composition 2 group (50 mg / mL) is significantly higher than that of the model group and the compositions 3 to 18, with a statistically significant difference (P<0.05). This indicates that even at a lower dose, the compositions 1 and 2 have a stronger promoting effect on hair follicle growth than the compositions 3 to 18.

[0083] Table 3. Number of hair follicles Example 2. Preparation of hair growth external solution Oil phase pretreatment: 3.5 grams of composition 1 prepared according to the method described in Example 1 were weighed, 6.0 grams of caprylic capric glyceride and 2.8 grams of PEG-40 hydrogenated castor oil were added, and the mixture was heated to about 42°C in a constant temperature water bath and stirred until uniform.

[0084] Water phase preparation: 75.0 grams of deionized water, 3.0 grams of glycerol, 4.0 grams of propylene glycol, 0.6 grams of lecithin, and 0.1 grams of sodium hyaluronate were weighed and added to a stirring container in sequence, and stirred at room temperature until completely dissolved to form a uniform and transparent water phase.

[0085] Emulsification step: under continuous stirring, the above oil phase was slowly added into the water phase at a constant rate, and sheared by a high-speed shearing emulsifier at a speed of about 7000 rpm for about 5 minutes, to obtain a microemulsion or transparent emulsion.

[0086] pH adjustment and post-treatment: after emulsification, the pH of the system was adjusted to 5.5-6.0 using a suitable pH adjuster (such as a triethanolamine and citric acid solution), then filtered through a 0.22 μm microporous filter, and finally filled into inert packaging containers under light-proof conditions to obtain a stable hair growth external preparation.

[0087] Example 3. Adjustment of composition preparation method On the basis of Preparation Example 1, only the preparation method of mulberry leaf extract was modified: using mulberry leaves as raw material, after washing and drying, the mulberry leaves were packed in a tank, 5 times the weight of water was used as solvent, heated to reflux extraction three times, each for 2 hours, the three extraction solutions were combined, concentrated and evaporated to a d=1.20 specific gravity extract, allowed to stand and precipitate, centrifuged, the solution was spray dried, pulverized and sieved to obtain the product.

[0088] Other methods were unchanged, and composition 19 was prepared. The method of Reference Example 1 was used for testing, and the results showed that the activity of composition 19 in promoting hair growth and increasing the number of hair follicles was comparable to that of composition 1, and far superior to that of compositions 3-18 (P<0.05).

Claims

1. A composition characterized in that, The composition comprises: (a) ginseng, biota leaf, angelica, rhizoma zedoariae, mulberry leaf, black ganoderma, radix falcaliae, false maozhi, and pteridium revolutum, each in an amount of 0.9-1.1 parts by weight; or (b) ginseng, biota leaf, angelica, rhizoma zedoariae, mulberry leaf, black ganoderma, radix falcaliae, Chinese liwhai, and stylophorum lasiicum, each in an amount of 0.9-1.1 parts by weight.

2. A composition characterized in that, The composition comprises: (a) ginseng extract, biota leaf extract, angelica extract, rhizoma zedoariae extract, mulberry leaf extract, black ganoderma extract, radix falcaliae extract, false maozhi extract, and pteridium revolutum extract, each in an amount of 0.9-1.1 parts by weight; or (b) ginseng extract, biota leaf extract, angelica extract, rhizoma zedoariae extract, mulberry leaf extract, black ganoderma extract, radix falcaliae extract, Chinese liwhai extract, and stylophorum lasiicum extract, each in an amount of 0.9-1.1 parts by weight. The preparation method of the extract is as follows: each raw material is pulverized, water reflux is performed, and filtration and concentration are performed to obtain the extract.

3. A composition characterized in that, The preparation method of the composition is as follows: (a) ginseng, biota leaf, angelica, rhizoma zedoariae, mulberry leaf, black ganoderma, radix falcaliae, false maozhi, and pteridium revolutum are taken; or, ginseng, biota leaf, angelica, rhizoma zedoariae, mulberry leaf, black ganoderma, radix falcaliae, Chinese liwhai, and stylophorum lasiicum are taken; (b) each plant raw material is pulverized, water reflux is performed, and filtration and concentration are performed to obtain a powder-like extract of each plant raw material; (c) 0.9-1.1 parts by weight of each extract is taken, mixed uniformly, 7-8 parts by weight of 75% ethanol is added, stirring is performed at room temperature, filtration is performed, and the filtrate is concentrated to obtain the composition.

4. The composition of claim 3, wherein The preparation method of the composition is as follows: (a) ginseng, biota leaf, angelica, rhizoma zedoariae, mulberry leaf, black ganoderma, radix falcaliae, false maozhi, and pteridium revolutum are taken; or, ginseng, biota leaf, angelica, rhizoma zedoariae, mulberry leaf, black ganoderma, radix falcaliae, Chinese liwhai, and stylophorum lasiicum are taken; (b) each plant raw material is pulverized, water reflux is performed, and filtration and concentration are performed to obtain a powder-like extract of each plant raw material; (c) 1.0 part by weight of each extract is taken, mixed uniformly, 7.2 parts by weight of 75% ethanol is added, stirring is performed at room temperature, filtration is performed, and the filtrate is concentrated to obtain the composition.

5. A composition characterized in that, The preparation method of the composition is as follows: (a) plant raw materials ginseng, biota leaf, angelica, rhizoma zedoariae, mulberry leaf, black ganoderma, radix falcaliae, false maozhi, and pteridium revolutum are taken; (b) each plant raw material in (a) is pulverized, 10 times the weight of water of each plant raw material is added for reflux for 5 hours, filtration is performed after cooling, and concentration is performed to obtain a powder-like extract of each plant raw material; (c) 1.0 part by weight of each extract in (b) is taken, mixed uniformly, 7.2 parts by weight of 75% ethanol is added, stirring is performed at room temperature, filtration is performed, the filtrate is concentrated to 1 / 3 of the volume under reduced pressure at 30°C, and freeze-drying is performed at -20°C to obtain the composition.

6. A composition characterized in that, The preparation method of the composition is as follows: (a) plant raw materials ginseng, biota leaf, angelica, rhizoma zedoariae, mulberry leaf, black ganoderma, radix falcaliae, Chinese liwhai, and stylophorum lasiicum are taken; (b) each plant raw material in (a) is pulverized, 10 times the weight of water of each plant raw material is added for reflux for 5 hours, filtration is performed after cooling, and concentration is performed to obtain a powder-like extract of each plant raw material; (c) 1.0 part by weight of each extract in (b) is taken, mixed uniformly, 7.2 parts by weight of 75% ethanol is added, stirring is performed at room temperature, filtration is performed, the filtrate is concentrated to 1 / 3 of the volume under reduced pressure at 30°C, and freeze-drying is performed at -20°C to obtain the composition. (c) each of the extracts in (b) was taken 1.0 parts by weight, mixed uniformly, 7.2 parts by weight of 75% ethanol was added, stirred at room temperature, filtered, the filtrate was concentrated to 1 / 3 volume at 30°C under reduced pressure, and then freeze-dried at -20°C to obtain the product.

7. A method for preparing a composition, the method comprising: (a) taking ginseng, biota leaf, angelica, Chinese knotweed, mulberry leaf, black ganoderma, radix falconis, false mao chi, and flat-branch pine leaf fern; or, taking ginseng, biota leaf, angelica, Chinese knotweed, mulberry leaf, black ganoderma, radix falconis, Chinese white, and Chinese brush tube grass; (b) each of the plant raw materials was ground, refluxed with water, cooled, filtered, and concentrated to obtain a powdered extract; (c) each of the extracts was taken 0.9-1.1 parts by weight, mixed uniformly, 7-8 parts by weight of 75% ethanol was added, stirred at room temperature, filtered, and concentrated to obtain the product.

8. A hair growth external liquid preparation, the preparation comprising the composition of any one of claims 1-6.

9. A pharmaceutical preparation, the pharmaceutical preparation comprising the composition of any one of claims 1-6.

10. Use of the composition of any one of claims 1-6 in the preparation of a medicament for promoting hair growth.

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