Method for extracting macleaya cordata pod alkaloid through ultrasonic-assisted enzymolysis

Abstract

The invention relates to the field of traditional Chinese veterinary medicine extraction, in particular to a method for extracting macleaya cordata pod alkaloid through ultrasonic-assisted enzymolysis. The macleaya cordata pod alkaloid is extracted by adopting ultrasonic-assisted enzymolysis. According to the method, the extraction rate of the alkaloid in the macleaya cordata pods is relatively high. The method provided by the invention has the advantages of simple required equipment, simplicity and convenience in operation, safety, high extraction rate and easiness in industrial production.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of traditional Chinese veterinary medicine extraction, and particularly relates to a method for ultrasonic-assisted enzymatic extraction of alkaloids in fruit pods of Macleaya cordata. BACKGROUND

[0002] Macleaya cordata is a perennial herb of Macleaya family and is a traditional Chinese medicinal material rich in alkaloids. Fruit pods of Macleaya cordata contain a large amount of isoquinoline alkaloids, mainly including protopine, sanguinarine, chelerythrine, alpha-allo-hydrastine and beta-allo-hydrastine, among which sanguinarine and chelerythrine have high content and have a wide range of biological activities such as antibacterial, anti-inflammatory, antitumor and anti-liver fibrosis.

[0003] At present, Macleaya cordata alkaloids are widely used in biological medicine, veterinary medicine, feed additives and biological pesticides. Although the traditional infiltration method, acid water extraction method and ethanol extraction method for extracting Macleaya cordata alkaloids are simple in operation, the extraction efficiency is low, the time is long, and a large amount of solvent is needed, which brings inconvenience to post-processing.

[0004] Therefore, it is urgent to find an extraction method which is simple in operation, high in extraction rate and suitable for large-scale extraction of Macleaya cordata alkaloids. SUMMARY

[0005] In order to solve the above technical problems in the prior art, the present application provides a method for ultrasonic-assisted enzymatic extraction of alkaloids in fruit pods of Macleaya cordata, which specifically comprises the following steps.

[0006] A method for ultrasonic-assisted enzymatic extraction of alkaloids in fruit pods of Macleaya cordata, comprising the following steps:

[0007] (1) The collected fruit pods of Macleaya cordata are naturally dried, crushed into powder and dried for use;

[0008] (2) The fruit pod powder of Macleaya cordata is uniformly mixed with a sulfuric acid aqueous solution at a ratio of 1:7 to 1:9;

[0009] (3) Under ultrasonic conditions, cellulase is added for enzymatic hydrolysis, and the obtained solution is cooled to room temperature and centrifuged to obtain a supernatant;

[0010] (4) Sodium hydroxide is added to the supernatant to adjust the pH value to 10-11, the solution is allowed to stand, and the filtrate is obtained by filtering the standing solution, thereby obtaining total alkaloids of Macleaya cordata.

[0011] Further, the drying in step (1) is drying at 60℃.

[0012] Further, the sulfuric acid aqueous solution is a sulfuric acid aqueous solution with a pH value of 3-5.

[0013] Further, the ultrasonic in step (3) has an ultrasonic power of 200W.

[0014] Further, the temperature during the ultrasonic treatment in step (3) is 30-50℃.

[0015] Further, the enzyme dosage in step (3) is 0.8-1.0% of the mass of the Boerhaave fruit pod powder.

[0016] Further, the enzymolysis time in step (3) is 1.0-3.0h.

[0017] Compared with the prior art, the technical effects of the present application are embodied in:

[0018] The present application uses ultrasonic-assisted enzymatic extraction of Boerhaave fruit pod alkaloids, wherein the ultrasonic treatment destroys the cell wall to achieve the effect of extracting Boerhaave fruit pod alkaloids; meanwhile, the biological enzyme dissolves the fiber components of the Boerhaave fruit pod cell wall to make the intracellular substances dissolve as soon as possible; the combination of the two can significantly reduce the extraction temperature of Boerhaave fruit pod alkaloids, shorten the extraction time, save the amount of solvent used, improve the extraction yield, and has no effect on the structure and physicochemical properties of the Boerhaave fruit pod alkaloid extract. The method of the present application has a high extraction rate of alkaloids in Boerhaave fruit pods. Moreover, the method of the present application requires simple equipment, is easy and safe to operate, has a high extraction rate, and is easy to industrialize. DETAILED DESCRIPTION

[0019] The technical solutions of the present application will be further limited in combination with specific embodiments, but the scope of protection is not limited to the description.

[0020] Example 1

[0021] (1) The collected Boerhaave fruit pods were naturally dried, crushed into powder by a crusher, and dried in an oven at 60℃ for use;

[0022] (2) 10g of Boerhaave fruit pod powder was taken and placed in a 100ml triangular flask, and a pH value of 4 sulfuric acid aqueous solution was added and mixed uniformly at a ratio of 1:8;

[0023] (3) Under the conditions of 200W and 40℃ ultrasonic, 0.9% of the mass of the Boerhaave fruit pod powder was added for enzymolysis for 2.0h, and then taken out and cooled to room temperature, and centrifuged to obtain the supernatant;

[0024] (4) Sodium hydroxide was added to the above supernatant to adjust the pH value to 10-11, and the solution was allowed to stand, and the filtrate was obtained after filtering the standing solution, thereby obtaining the total Boerhaave alkaloids.

[0025] Example 2

[0026] (1) The collected Boerhaave fruit pods were naturally dried, crushed into powder by a crusher, and dried in an oven at 60℃ for use;

[0027] (2) Take 10g of the fruit pod of the plant, place it in a 100ml flask, and add a sulfuric acid aqueous solution with a pH value of 3, mixed uniformly at a ratio of 1:7;

[0028] (3) Under the condition of ultrasonic with 200W and 30℃, add cellulase with a mass of 0.8% of the fruit pod of the plant, and enzymatically hydrolyze for 1.0h, take out and cool to room temperature, and centrifuge to obtain supernatant;

[0029] (4) Add sodium hydroxide to the supernatant to adjust the pH value to 10-11, stand, filter the solution after standing, and take the residue, to obtain total alkaloids of the plant.

[0030] Example 3

[0031] (1) Dry the fruit pod of the plant collected by natural airing, crush it into powder by a crusher, and place it in an oven to dry at 60℃ for standby;

[0032] (2) Take 10g of the fruit pod of the plant, place it in a 100ml flask, and add a sulfuric acid aqueous solution with a pH value of 5, mixed uniformly at a ratio of 1:9;

[0033] (3) Under the condition of ultrasonic with 200W and 50℃, add cellulase with a mass of 1.0% of the fruit pod of the plant, and enzymatically hydrolyze for 3.0h, take out and cool to room temperature, and centrifuge to obtain supernatant;

[0034] (4) Add sodium hydroxide to the supernatant to adjust the pH value to 10-11, stand, filter the solution after standing, and take the residue, to obtain total alkaloids of the plant.

[0035] Finally, it should be pointed out that the above examples are only more representative examples of the present application. Obviously, the technical solutions of the present application are not limited to the above examples, and there can be many variations. All variations that can be directly derived or inferred by those skilled in the art from the disclosed content of the present application should be considered as falling within the protection scope of the present application.

Claims

1. A method for ultrasonic-assisted enzymatic extraction of alkaloids from the fruit capsules of Macuna pruriens, characterized by, It comprises the following steps: (1) The collected Boerhaave fruit hulls are naturally dried, crushed into powder, and dried for later use; (2) The Boerhaave fruit hull powder is mixed with a sulfuric acid aqueous solution at a ratio of 1:7-1:9; (3) Under ultrasonic conditions, cellulase is added for enzymolysis, and the obtained solution is cooled to room temperature, centrifuged, and the supernatant is obtained; (4) Sodium hydroxide is added to the supernatant to adjust the pH value to 10-11, the solution is allowed to stand, and the obtained filtrate is obtained, namely the total alkaloid of Boerhaave.

2. The method for ultrasonic-assisted enzymatic extraction of alkaloids from the fruit capsule of Macuna pruniaria according to claim 1, characterized in that, The drying in step (1) is drying at 60℃.

3. The method for ultrasonic-assisted enzymatic extraction of alkaloids from the fruit capsule of Macuna pruniaria according to claim 1, characterized in that, The sulfuric acid aqueous solution is a sulfuric acid aqueous solution with a pH value of 3-5.

4. The method for ultrasonic-assisted enzymatic extraction of alkaloids from the fruit capsule of Macuna pruniaria according to claim 1, characterized in that, The ultrasonic power in step (3) is 200W.

5. The method of ultrasonic-assisted enzymatic extraction of alkaloids from the fruit capsule of Macuna pruniaria according to claim 1, characterized by, The temperature during ultrasonic treatment in step (3) is 30-50℃.

6. The method of ultrasonic-assisted enzymatic extraction of alkaloids from the fruit capsule of Macuna pruniaria according to claim 1, characterized by that, The cellulase in step (3) is used in an amount of 0.8%-1.0% of the mass of the Boerhaave fruit hull powder.

7. The method of ultrasonic assisted enzymatic extraction of alkaloids from the fruit capsule of Macuna pruniaria according to claim 1, characterized by, The enzymolysis time in step (3) is 1.0-3.0h.