An anti-inflammatory polypeptide from buthus martensii koch and screening method and application thereof

Through systematic screening and validation, the anti-inflammatory peptides G4, G5, and G17 of *Scorpio scorpio*, especially G5, have solved the problem of insufficient research on the structure and function of *Scorpio scorpio* peptides, achieved significant inhibition of microglial inflammatory factors, and provided a basis for their application in anti-neuroinflammatory drugs.

CN121064287BActive Publication Date: 2026-01-27NANJING UNIV OF TRADITIONAL CHINESE MEDICINE
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Patent Information

Application Number
CN202511612676.0
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2025-11-06
Publication Date
2026-01-27
Estimated Expiration
2045-11-06

AI Technical Summary

Technical Problem

The lack of systematic screening of East Asian scorpion peptides and the insufficient research on the structure and function of individual peptides in the current technology limits their clinical application and exploration of molecular mechanisms in the fields of analgesia, anti-inflammation, antibacterial, and neuroprotection.

Method used

Anti-inflammatory peptides G4, G5, and G17 from *Scorpio scorpionensis* were screened using liquid nitrogen grinding, lysis buffer extraction, nano-liquid phase separation, mass spectrometry, solid-phase synthesis, and qPCR. Their bioactivity and toxicity were predicted using NCBI, PeptideRanker, ToxIBTL, and Algpred 2.0. Peptides with anti-inflammatory properties were screened, and their inhibitory effects on microglial inflammatory factors were verified.

Benefits of technology

The screened anti-inflammatory peptides G4, G5, and G17 from the East Asian scorpion, especially G5, can significantly inhibit the expression of inflammatory factors in microglia, providing a theoretical basis for their pharmaceutical development and revealing a potential anti-neuroinflammatory mechanism, making them suitable for anti-neuritis drugs.

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Abstract

The application discloses an anti-inflammatory polypeptide of Buthus martensii Karsch and a screening method and application thereof, and belongs to the technical field of traditional Chinese medicinal material polypeptides. The anti-inflammatory polypeptide of Buthus martensii Karsch is one of G4, G5 and G17. The nanoElute 2 nanoliter liquid phase separation, timsTOFPro2 mass spectrum analysis and BPS Novor library searching analysis are used to detect the polypeptide extract of Buthus martensii Karsch, screen the polypeptide of Buthus martensii Karsch, and further synthesize the candidate polypeptide screened above by using a solid-phase synthesis method; the effect of the synthesized candidate polypeptide on inhibition of microglial cell inflammation is evaluated by using a qPCR method, and then the anti-inflammatory polypeptide of Buthus martensii Karsch is screened. The anti-inflammatory polypeptides G4, G5 and G17 of Buthus martensii Karsch screened by the method, especially the G5, can significantly inhibit microglial cell inflammation in a concentration-dependent manner, provide a theoretical basis for medicinal development of the polypeptide of Buthus martensii Karsch, and reveal a potential anti-neuroinflammatory mechanism.
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