Cosmetic composition of yeast cell wall

Abstract

The present invention addresses the problem of using yeast-derived cell walls to provide a material which has a skin wrinkle improving effect and is further useful as a tactile sensation improver when applied to the skin. The present inventors have completed the present invention by finding that a cosmetic composition having a skin wrinkle improving effect, a moisture retaining effect, and a skin barrier effect can be provided by using a yeast cell wall-containing composition having a yeast cell wall structure, and also can be used as a tactile sensation improving agent through an in-depth study. Further, the composition comprising the yeast cell wall of the present invention can be extracted from a food yeast, and thus belongs to a food microorganism source and can be safely used.

Description

TECHNICAL FIELD

[0001] The present application relates to a cosmetic composition containing a yeast-derived material. BACKGROUND

[0002] In order to maintain healthy skin, care is performed using cosmetics such as toners, emulsions, and the like, or external medicines. In terms of skin improvement and the like, a material derived from a natural source is sometimes used, and a microorganism-derived material such as lactic acid bacteria, yeast, and the like is utilized.

[0003] For example, it has been reported that β-glucan derived from yeast, basidiomycetes, and the like has an effect of preventing aging of aged skin, moisturizing, skin beautifying, and the like (Patent Document 1). β - β-glucan has an effect of preventing aging of aged skin, moisturizing, skin beautifying, and the like (Patent Document 1).

[0004] PRIOR ART DOCUMENTS PATENT DOCUMENTS Patent Document 1: Japanese Patent Application Laid-Open No. 2005-15348 SUMMARY OF THE INVENTION

[0005] PROBLEMS TO BE SOLVED BY THE INVENTION The present application relates to a cosmetic composition containing a yeast-derived material.

[0006] MEANS FOR SOLVING THE PROBLEMS The present inventors and the like have conducted intensive studies, and as a result, have found that the above problems can be solved by utilizing a yeast cell wall, thereby completing the present application.

[0007] That is, the present application is (1) A skin external composition for moisturizing, characterized by comprising a yeast cell wall-containing composition that maintains the shape of a yeast cell wall.

[0008] (2) A skin external composition for wrinkle improvement, characterized by comprising a yeast cell wall-containing composition that maintains the shape of a yeast cell wall.

[0009] (3) A skin external composition having moisture retention durability and barrier properties, characterized by comprising a yeast cell wall-containing composition that maintains the shape of a yeast cell wall.

[0010] (4) A skin external composition having sebum adsorption, characterized by comprising a yeast cell wall-containing composition that maintains the shape of a yeast cell wall.

[0011] (5) A skin external composition of a whitening-inhibiting type, characterized by comprising a yeast cell wall-containing composition that maintains the shape of a yeast cell wall.

[0012] (6) A skin external preparation characterized by comprising a yeast cell wall-containing composition which maintains the shape of a yeast cell wall of Saccharomyces.

[0013] (7) A skin external preparation characterized by comprising a yeast cell wall which maintains the shape of a yeast cell wall and has an average particle diameter of 5 to 10 μ m.

[0014] (8) A method for maintaining the barrier property of skin and providing moisture to skin, characterized by using a skin external preparation comprising a yeast cell wall-containing composition which maintains the shape of a yeast cell wall.

[0015] Effects of the Invention According to the present application, a cosmetic composition having skin wrinkle-improving effect, moisture-retaining effect and skin barrier effect can be provided, and can also be used as a feel-improving agent.

[0016] Further, the yeast cell wall-containing composition of the present application can be extracted from food yeast, and thus belongs to a food microorganism source, and can be used safely. DETAILED DESCRIPTION

[0017] The present application is a yeast cell wall-containing composition which maintains the shape of a yeast cell wall. In the present application, "maintaining the shape of a yeast cell wall" means maintaining the structure of a yeast cell wall in part, and does not necessarily mean maintaining the shape of a yeast cell. Both the case where a yeast cell wall maintains the shape of a yeast cell and the case where a yeast cell wall is partially missing from the shape of a yeast cell are defined as "a yeast cell wall-containing composition which maintains the shape of a yeast cell wall".

[0018] The yeast used for the production of the yeast cell wall-containing composition of the present application can be any yeast belonging to the classification of yeast. Any yeast can be used. Preferably, a yeast commonly used for food production and the like is used. Specifically, yeast belonging to the genus Saccharomyces, such as Saccharomyces cerevisiae, Saccharomyces pastrianus, Saccharomyces rouxii, Saccharomyces carlsbergensis, and the like, yeast belonging to the genus Candida, such as Candida utilis, Candida tropicalis, Candida lipolytica, Candida flaveri, and the like can be exemplified. Preferably, yeast belonging to the genus Saccharomyces, such as Saccharomyces cerevisiae, Saccharomyces pastrianus, Saccharomyces rouxii, Saccharomyces carlsbergensis, and the like is used, and more preferably, Saccharomyces cerevisiae or Saccharomyces pastrianus is used. These yeasts can be used alone or two or more selected from the above can be used in combination. Among them, beer yeast is particularly preferred. In the case of using beer yeast, in order to remove the bitterness of beer, it is preferred to use yeast cells subjected to debittering treatment such as washing with an aqueous alkali solution. The debittering treatment can be performed using a method known to those skilled in the art, and is not particularly limited. If specifically exemplified, the method described in Japanese Patent Application Laid-Open No. 55-162983 can be followed.

[0019] The yeast cell wall-containing composition used in the present application can be produced using a method known to those skilled in the art. In general, the yeast cell wall component used in the present application can be produced by disrupting yeast cells and removing the intracellular components.

[0020] The following exemplifies a method for producing the yeast cell wall-containing composition used in the present application.

[0021] The yeast cells are subjected to digestion treatment with enzymes. The digestion treatment of the yeast cells can be performed using a self-digestion method using the intrinsic enzymes of the yeast cells, or a method in which a protease, a nuclease, a carbohydrase, and the like are added from the outside. β- enzyme addition method of enzymes such as glucanase, esterase, or lipase. In addition, the above digestion treatment can also be performed by combining the autodigestion method and the enzyme addition method. In addition, the digestion treatment can also be performed using a treatment other than the above, as long as it is a method generally used in the preparation of yeast extract, and any method can be used. In addition, before the digestion treatment, a pretreatment can be performed using a high-pressure homogenizer or the like, with the purpose of reducing the digestion treatment time of the above enzymes, as necessary, with physical destruction of the cell wall. The pretreatment using a high-pressure homogenizer can be performed at a pressure of, for example, 100 to 1000 kg / cm2. After the digestion treatment, the soluble cell component of the above enzyme treatment is removed by centrifugal separation or the like, and then the granules are treated with an acidic aqueous solution, and the yeast cell residue obtained as an insoluble component is recovered by centrifugal separation or the like, to produce the composition containing the cell wall of the present application. As the acidic aqueous solution, an acid such as hydrochloric acid, sulfuric acid, nitric acid, or the like, at 0.01 to 2 N, preferably 0.1 to 0.5 N, can be used. The acid treatment is preferably performed with heating to 50°C or higher, and more preferably with heating to 80°C or higher.

[0022] In addition, in the present application, a yeast extract residue produced in a known yeast extract production process can also be used. The obtained composition containing the cell wall can be used as it is by being suspended in a solvent such as water or ethanol. The composition containing the cell wall can be used in a solid form after drying or in a powder form.

[0023] The composition containing the cell wall adjusted in this way can maintain the form of the yeast cell wall. Figure 1 In addition, the average particle diameter of the composition containing the cell wall of the present application is 5 to 15 μ m. In addition, the average particle diameter of the present application is measured using a laser diffraction type particle size distribution measuring device (SALD-300V, manufactured by Shimadzu Corporation) using a wet method, with the median particle diameter as the particle diameter.

[0024] In addition, the composition containing the cell wall of the present application contains β - dietary fiber containing glucan. The content of the dietary fiber in the composition of the present application is 40% by weight or more, preferably 45% by weight or more, and more preferably 50% by weight or more, in the solid component. In addition, the content of the β - glucan in the dietary fiber is 30% by weight or more, preferably 40% by weight or more, and more preferably 45% by weight or more.

[0025] The cell wall-containing composition of the present application can be utilized as a cosmetic composition. As a method of utilization, for example, the product of the present application can be utilized by being formulated in a cosmetic composition. The formulation amount of the cell wall-containing composition of the present application in a cosmetic composition is arbitrary, and can be a formulation amount that can be appropriately selected by one skilled in the art. In order to make the anti-wrinkle effect, wrinkle improvement effect, moisture retention durability and barrier property, and whitening suppression effect of the present application more excellent, it is desirable that the cell wall-containing composition of the present application is contained in a cosmetic composition at 0.01 to 30% by weight, preferably 0.05 to 15% by weight, and more preferably 0.1 to 10% by weight.

[0026] In using the cosmetic composition of the present application, in addition to the present application, general ingredients that can be formulated in a cosmetic composition can also be formulated. For example, various effective ingredients such as oil agents, alcohols, ethers, surfactants, metal ion blocking agents, pearl agents, amino acids, organic amines, water-soluble polymers, polymer emulsions, pH adjustors, vitamins, antioxidants, preservatives, water-soluble polymers, perfumes, ultraviolet absorbers, ultraviolet blockers, and the like can be exemplified. In addition, various vitamins, whitening agents, amino acids, anti-inflammatory agents, and the like that are formulated in a cosmetic as an efficacy ingredient can also be formulated.

[0027] As the type of the cosmetic of the present application, a skin external agent including a base cosmetic, a makeup cosmetic, a fragrance cosmetic, a body cosmetic, an ointment, and the like can be exemplified. It can be manufactured by a conventional method. As the dosage form of the cosmetic or skin external agent of the present application, an emulsified cosmetic of an oil-in-water (O / W) type, a water-in-oil (W / O) type, a W / O / W type, an O / W / O type, an oily cosmetic, a solid cosmetic, a liquid cosmetic, a paste cosmetic, a stick cosmetic, a volatile oil type cosmetic, a powder cosmetic, a jelly cosmetic, a gel cosmetic, a paste cosmetic, an emulsified polymer type cosmetic, a sheet cosmetic, a mist cosmetic, a spray type cosmetic, and the like can be exemplified as preferable dosage forms.

[0028] Hereinafter, the present application will be described in more detail by citing examples, but the present application is not limited to these examples.

[0029] Example 1 (Manufacture of the composition of the present application) Example 1 A beer yeast slurry obtained by beer production was suspended in water so that the solid content would be 5% by weight, using the sludge-like live yeast obtained by centrifugal separation at 4500 rpm for 10 minutes. After subjecting the suspension to self-digestion at 50°C for 17 hours, it was again centrifugally separated to remove the soluble intracellular components, and the self-digested residue was used as a cell wall-containing composition. The average particle diameter of the cell wall-containing composition was 5.7 μ m, the dietary fiber content in the solid content was 51% by weight, and the glucan content in the dietary fiber was 45% by weight. This composition was used in the following tests as Production Example 1. β - glucan content was 45% by weight. This composition was used in the following tests as Production Example 1.

[0030] (Human Test) Twenty subjects (female, average age 49.3 years) were instructed to apply the test product twice a day, and various measurements of the skin were performed before the test and 28 days later. During the test, an emulsion containing 3% of the composition of Production Example 1 was used, adjusted according to the formulation of Table 1 using a conventional method. The application method and the evaluation method are shown below.

[0031] [Table 1]

[0032] Application Method After washing the face, the skin was conditioned with the usual cosmetic water, and 4 pressings of the test product were taken on the hand and applied evenly to the entire face. Again, 4 pressings were taken on the hand and applied to the entire face (wash face → cosmetic water → test product first time → test product second time).

[0033] Evaluation Method After washing the face, the skin was conditioned with the usual cosmetic water, and 4 pressings of the test product were taken on the hand and applied evenly to the entire face. Again, 4 pressings were taken on the hand and applied to the entire face (wash face → cosmetic water → test product first time → test product second time). The results are shown in Table 2. The stratum corneum water content was about 1.3 times, showing a clear increase. In addition, in terms of texture, a clear increase in the texture volume rate was also observed by replica analysis. The wrinkle improvement effect was measured using the VISIA system, and the results showed that the number of wrinkles was significantly reduced after the test.

[0034] [Table 2]

[0035] (Sensory Test) In addition, the composition of Production Example 1 was formulated into a sunscreen, a foundation containing a pigment, and the like according to a conventional method, and compared with a product to which the product of the present application was not formulated, evaluation of skin touch and the like was performed by sensory evaluation. As a result, the sensory evaluator evaluated that the hand feeling was improved to be very smooth. It was confirmed that the yeast cell wall could be utilized as a touch improver.

[0036] According to the results of the sensory test, further test of the touch improver on the skin applicability was performed. The composition of Production Example 1 was formulated at 3% in a sunscreen formulation containing a solid pigment / particle (titanium oxide). In addition, as a comparative object, a control group (unformulated product), an acrylic resin (PMMA) formulation which is usually used for cosmetics were adjusted similarly. Each formulation was applied to the arm, and spreaded evenly with the finger pad.

[0037] ( Figure 2 Legend: C: control group, YV: product of the present application, P: PMMA) The results are shown in Table 1. Figure 2 Compared with the unformulated sunscreen, the whitening of the formulation of Production Example 1 was reduced. On the other hand, PMMA having a similar particle size could not prevent whitening, and in addition, the skin touch was also improved as in the sensory evaluation of the previous paragraph.

[0038] (Moisture retention) The composition of Production Example 1 was thinly applied to filter paper, and the moisture retention Figure 3 Initial value: when the composition of Production Example 1 was not dried (water was removed by centrifugation): 0.2891 g After drying at 40°C: 0.0340 g After drying at 110°C: 0.0237 g Assuming that it was completely dried at 110°C, 0.0340-0.0237=0.0103 g of water was retained. The moisture retention rate was

[0039] In addition, it was also discussed whether the product of the present application could balance moisture retention and skin barrier properties (barrier properties).

[0040] Sample: 1% solution of the composition of Production Example 1 Electron microscope shooting method: 〈Sample pretreatment〉 After the sample solution was injected into a culture dish, it was stored in a -80°C freezer for 30 minutes or more Vacuum freeze-dried for 24 hours 〈Shooting conditions〉 Sample adhesion method: adhered to a conductive carbon double-sided tape at 0.7-1.5 mg / cm2​​ Antistatic conditions: Coating material: Au; Film thickness: 5 nm; Apparatus: JEC-3000FC Measurement conditions: Voltage: 15kV, Mode: SED High Vacuum, Apparatus: JCM-7000 The results show that, Figure 4 As shown, the product of this invention also exhibits a dense structure in terms of applicable concentrations and dosages for cosmetic use. Skin can be damaged by external factors such as air pollutants like PM2.5 and pollen; however, the dense structure formed by the product of this invention is expected to protect the skin from these external factors.

[0041] In addition, tests were conducted to determine whether it has skin-barrier properties.

[0042] An aqueous solution containing 0%, 1.25%, and 2.5% of the present invention, and an aqueous solution containing 3% of the present invention were applied to the inner side of the forearm. After drying, the transdermal water evaporation volume (TEWL) was measured.

[0043] The results showed that it had a barrier effect that reduced transdermal water evaporation. Figure 5 , 6 ) Based on the above results, it can be confirmed that the product of this invention can be used as a cosmetic material that balances moisture retention and skin barrier function. Attached Figure Description

[0044] [ Figure 1 A photograph of an example of a composition containing yeast cell walls that maintains the shape of yeast cell walls.

[0045] [ Figure 2 A skin-feel improver.

[0046] [ Figure 3 Moisture retention test using filter paper.

[0047] [ Figure 4 Electron microscope image.

[0048] [ Figure 5 Skin barrier properties verification results.

[0049] [ Figure 6 Skin barrier properties verification results.

Claims

1. A moisturizing topical skin composition, characterized in that, A composition containing yeast cell walls that maintains the shape of the yeast cell walls.

2. A wrinkle ameliorating skin external preparation, characterized by, A composition containing yeast cell walls that maintains the shape of the yeast cell walls.

3. A skin external preparation having moisture retention durability and barrier property, characterized by, A composition containing yeast cell walls that maintains the shape of the yeast cell walls.

4. A skin external composition having sebum adsorption, characterized by, A composition containing yeast cell walls that maintains the shape of the yeast cell walls.

5. A topical skin composition for inhibiting whitening, characterized in that, A composition containing yeast cell walls that maintains the shape of the yeast cell walls.

6. A skin external composition, characterized by, A composition containing yeast cell walls that maintains the shape of the yeast cell walls.

7. A skin external composition, characterized by, The average particle size of the yeast cell wall selected from one of claims 1 to 6 maintaining the shape of the yeast cell wall is 5 to 10 μ m.

8. A method of maintaining the barrier properties of the skin and providing moisture to the skin, characterized by, Use of a skin topical composition comprising a composition containing yeast cell walls that maintains the shape of the yeast cell walls.

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