Composition with soothing function, spray prepared from composition and preparation method of spray

Abstract

The invention provides a composition with a soothing effect, a spray prepared from the composition and a preparation method of the spray. The composition with the soothing effect is prepared from the following raw materials in percentage by mass: 0.05 to 5 percent of fibroin composite microsphere aqueous solution serving as a skin protecting agent, 0.2 to 5 percent of skin conditioning agent, 5 to 10 percent of humectant, 0.1 to 1 percent of emollient and the balance of water. Wherein the fibroin composite microspheres have the characteristic of transdermal absorption, so that the spray prepared from the composition can go deep into the skin bottom layer and deliver skin conditioning and moisturizing components, the purpose of instantly, rapidly and effectively providing moisture supplement for the skin is achieved, skin discomfort can be rapidly relieved, and then the skin barrier toughness is enhanced.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of cosmetic preparation, and particularly relates to a composition with soothing effect and a spray prepared therefrom and a preparation method. BACKGROUND

[0002] The stratum corneum, the outermost layer of the skin, is a critical barrier to maintain moisture and resist irritation. When exposed to external attacks, intercellular lipids are lost, natural moisturizing factor (NMF) is reduced, trans-epidermal water loss (TEWL) is intensified, the barrier appears micro-cracks, and nerve endings are more susceptible to irritation, thereby triggering dryness, tightness and sensitive discomfort. Traditional skin care procedures (such as applying serum, cream) are effective but cannot be reapplied at any time.

[0003] In today's fast-paced living environment, the skin is continuously challenged by environmental pollution, digital blue light, stress, and seasonal sensitivity, leading to impaired skin barrier function, accelerated water loss, and frequent occurrence of redness, itching and other discomfort conditions. Therefore, there is an urgent need in the market for a solution that can instantly, conveniently and effectively provide moisture replenishment and state stabilization for the skin.

[0004] The core technical advantage of the spray form is that it can instantly form a uniform hydration film on the skin surface, replenish water and moisturizing agents (such as glycerin, panthenol, sodium hyaluronate), rapidly reduce epidermal temperature, relieve burning sensation, and physically isolate external stimuli to create a valuable window period for barrier self-repair. At the same time, in order to ensure the stability and permeability of active ingredients, nanotechnology and microemulsification technology are applied to make the effective ingredients more easily absorbed.

[0005] Therefore, the development of a soothing spray product is an inevitable technical direction to meet the instant and efficient needs of modern skin care. Through instant cooling and calming, replenishing water and moisturizing factors, and delivering high-concentration soothing active ingredients, multiple pathways work synergistically to quickly relieve skin discomfort and strengthen barrier resilience. This is not only an important supplement to the daily skin care process, but also an innovative technical solution to maintain skin health and stability in various internal and external stress environments. SUMMARY

[0006] In view of the deficiencies in the prior art, the purpose of the present application is to provide a silk fibroin composite microsphere and a preparation method and application thereof.

[0007] To achieve this purpose, the present application adopts the following technical solutions:

[0008] The application provides a composition with soothing effect, raw materials of the composition include: 0.05%-5% of a silk fibroin composite microsphere aqueous solution as a skin protective agent, 0.2%-5% of a skin conditioning agent, 5%-10% of a humectant, 0.1%-1% of an emollient, and the balance is water.

[0009] The composition contains the silk fibroin composite microsphere aqueous solution, the composite microsphere can realize transdermal absorption of effective components, penetrate into the skin muscle layer, deliver skin conditioning and moisturizing components, instantly and effectively provide moisture supplement for the skin, rapidly relieve skin discomfort, and strengthen barrier toughness.

[0010] The mass percentage of the silk fibroin composite microsphere aqueous solution is 0.05%-5%, for example, 0.05%, 0.1%, 0.5%, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5% or the like. The mass percentage of the skin conditioning agent is 0.2%-5%, for example, 0.2%, 0.5%, 0.8%, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5% or the like. The mass percentage of the humectant is 5%-10%, for example, 5%, 6%, 7%, 8%, 9%, 10%. The mass percentage of the emollient is 0.1%-1%, for example, 0.1%, 0.2%, 0.5%, 0.8%, 1.0% or the like.

[0011] As a preferred technical solution of the application, the silk fibroin composite microsphere is prepared by the following method: silk fibroin and polydeoxyribonucleic acid (PDRN) are mutually penetrated, intertwined and folded and compressed under the action of polylysine to obtain the silk fibroin composite microsphere.

[0012] It is obtained by molecular docking calculation simulation that there is a supramolecular interaction between PDRN and silk fibroin, and then the supramolecular self-assembly between silk fibroin and PDRN is realized by using the interaction, and the obtained silk fibroin composite microsphere can penetrate the cell membrane and enter the cell to achieve the effect of transdermal absorption.

[0013] Preferably, the average particle size of the silk fibroin composite microsphere is 50 nm-500 nm, for example, 50 nm, 100 nm, 150 nm, 200 nm, 250 nm, 300 nm, 320 nm, 330 nm, 340 nm, 350 nm, 360 nm, 380 nm, 400 nm, 420 nm, 450 nm, 480 nm or 500 nm or the like.

[0014] As a preferred technical solution of the present application, the mass ratio of silk fibroin to polylysine in the raw material for preparing the silk fibroin composite microspheres is 1:(0.006-0.2), for example, it can be 1:0.006, 1:0.01, 1:0.02, 1:0.05, 1:0.1, 1:0.15, or 1:0.2, etc.

[0015] Preferably, the mass ratio of silk fibroin to polydeoxyribonucleic acid is 1:(1-10), for example, it can be 1:1, 1:2, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, 1:9, or 1:10, etc.

[0016] Preferably, the mass ratio of polylysine to polydeoxyribonucleic acid is 1:(5-150), for example, it can be 1:5, 1:5, 1:10, 1:20, 1:50, 1:60, 1:80, 1:100, 1:120, or 1:150, etc.

[0017] Preferably, the mass percentage of silk fibroin in the aqueous solution of the silk fibroin composite microspheres is 1%-5%, for example, it can be 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, or 5%, etc.

[0018] Preferably, the mass percentage of polylysine in the aqueous solution of the silk fibroin composite microspheres is 0.01%-1%, and the polylysine is used to provide a cationic environment, and the mass percentage thereof can be 0.01%, 0.02%, 0.05%, 0.1%, 0.2%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, or 1%, etc.

[0019] Preferably, the mass percentage of PDRN in the aqueous solution of the silk fibroin composite microspheres is 1%-5%, for example, it can be 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, or 5%, etc.

[0020] Preferably, the polylysine includes basic polylysine and / or modified polylysine.

[0021] Preferably, the polylysine is basic polylysine and modified polylysine with a mass ratio of 1:(0.5-0.8), for example, it can be 1:0.5, 1:0.55, 1:0.6, 1:0.65, 1:0.7, 1:0.75, or 1:0.8, etc.

[0022] The basic polylysine can provide a cationic environment and play a role in triggering the formation of composite microparticles. The use of modified polylysine to partially replace basic polylysine further improves the transdermal absorption rate.

[0023] Preferably, the preparation of the modified polylysine comprises the following steps:

[0024] The phospholipid polyethylene glycol carboxyl is added to water and stirred uniformly, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), N-hydroxysuccinimide (NHS) is added, stirred at 25-30°C for 5-10 min, dropped into the buffer solution containing the basic polylysine (the buffer solution is phosphate buffer solution, pH is 7.4), stirred at 25-30°C for 20-24 h, purified, freeze-dried, and the modified polylysine is obtained.

[0025] As a preferred technical solution of the present application, the aqueous solution of silk fibroin composite microspheres further comprises a pH adjusting agent, and the pH of the aqueous solution of silk fibroin composite microspheres is 5.5-6.5, for example, it can be 5.5, 5.6, 5.8, 6.0, 6.2, 6.3 or 6.5, etc.

[0026] Preferably, the pH adjusting agent comprises any one or a combination of at least two of citric acid, lactic acid, malic acid, tartaric acid or glycolic acid, preferably citric acid.

[0027] Preferably, the aqueous solution of silk fibroin composite microspheres further comprises a thickening agent, and the content of the thickening agent in the aqueous solution of silk fibroin composite microspheres is 0.5%-1%, for example, it can be 0.5%, 0.6%, 0.7%, 0.8%, 0.9% or 1%, etc.

[0028] Preferably, the thickening agent is hydroxyethyl cellulose.

[0029] In the present application, silk fibroin, PDRN and polylysine can form the silk fibroin composite microspheres, and optional components such as thickening agents and pH adjusting agents can be added to the silk fibroin composite microspheres, which are easier to store and can better compatible with other components. The use amount of the thickening agent and the pH adjusting agent can be adjusted according to the subsequent application.

[0030] As a preferred technical solution of the present application, the skin conditioning agent comprises any one or a combination of at least two of hydrogenated lecithin, silk amino acid or glycerol glucoside.

[0031] In the present application, the skin conditioning agent is preferably a combination of hydrogenated lecithin, silk amino acid and glycerol glucoside, and the ratio can be 1:(0.5-1.5):(0.001-0.01).

[0032] The hydrogenated lecithin has strong hydrophilicity and moisturizing property, can effectively help the skin to maintain moisture, reduce water loss, and form a moisturizing barrier. The hydrogenated lecithin can penetrate into the deep layer of the stratum corneum, promote the proliferation of keratinocytes, increase the density of the skin, repair and enhance the skin barrier, and reduce the damage of external injury to the skin.

[0033] Silk amino acids, INCI name: SILK AMINO ACIDS, CAS 96690-41-4, are a mixture of amino acids extracted from natural silk, which have high physiological activity and human compatibility. Silk amino acids are a high-activity cosmetic skin care raw material with the functions of moisturizing, nourishing the skin, inhibiting melanin formation, promoting skin tissue regeneration, preventing cracking and chemical damage, etc., and are widely used in cosmetics.

[0034] Glycerol glucoside has the functions of moisturizing and antioxidant, and can also protect the stratum corneum from ultraviolet damage, help cell regeneration after sun exposure, prevent collagen cross-linking caused by ultraviolet, inhibit cytokine formation, prevent inflammation, and also has obvious effects on restoring skin elasticity, reducing skin redness, barrier repair and reducing wrinkles.

[0035] Preferably, the moisturizing agent includes any one or a combination of at least two of butylene glycol, trehalose, glycerol, 1,2-hexanediol, 1,2-pentanediol or hyaluronic acid.

[0036] Preferably, the emollient is any one or a combination of at least two of caprylic / capric triglyceride, isopropyl myristate, isopropyl palmitate or dicaprylyl carbonate, preferably caprylic / capric triglyceride.

[0037] As a preferred technical solution of the present application, the spray further comprises an antioxidant.

[0038] Preferably, the mass percentage of the antioxidant is 0.1%-1%, for example, it can be 0.1%, 0.2%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9% or 1%, etc.

[0039] Preferably, the antioxidant is any one or a combination of at least two of p-hydroxyacetophenone, caprylhydroxamic acid, tocopherol, ubiquinone or ascorbic acid, preferably p-hydroxyacetophenone.

[0040] In a second aspect, the present application provides a spray comprising the composition according to the first aspect.

[0041] The spray in the present application can use nitrogen as a propellant and be prepared into a binary package valve aerosol, that is, the agent is contained in the capsule bag of the valve, and the propellant is filled in the gap between the capsule bag and the tank body, the agent and the propellant are isolated from each other and not mixed, and when used, the capsule bag is extruded by the propellant to release the agent in a predetermined form.

[0042] The product according to the present application is used in a spraying manner, and the sprayed material can be in a solid, liquid or gaseous state, and the sprayed shape can be mist, foam, powder, micelle, etc.

[0043] In a third aspect, the present application also provides a preparation method of the spray as described in the second aspect, and the preparation method comprises the following steps:

[0044] The formula amount of the aqueous solution of silk fibroin composite microspheres, the skin conditioner, the moisturizing agent, the emollient, the optional antioxidant and water are mixed, stirred, and sampled and detected to be qualified, and then filled to obtain the spray.

[0045] As a preferred technical solution of the present application, the preparation method of the aqueous solution of silk fibroin composite microspheres is as follows:

[0046] The polydeoxyribonucleic acid is added into water and stirred uniformly to obtain an aqueous solution of polydeoxyribonucleic acid;

[0047] The silk fibroin solution is diluted, mixed with the aqueous solution of polydeoxyribonucleic acid, and the aqueous solution of polylysine is added to obtain a composite solution system, which is stirred and mixed;

[0048] The optional pH adjuster and thickening agent are further added, and the aqueous solution of silk fibroin composite microspheres is obtained after stirring.

[0049] As a preferred technical solution of the present application, the moisturizing agent is pre-dissolved at 80-90°C (for example, 80°C, 81°C, 82°C, 83°C, 84°C, 85°C, 86°C, 87°C, 88°C, 89°C or 90°C, etc.).

[0050] Preferably, the emollient is pre-dissolved at 40-60°C (for example, 40°C, 42°C, 44°C, 45°C, 48°C, 50°C, 52°C, 55°C, 58°C or 60°C, etc.).

[0051] Preferably, the antioxidant is pre-dissolved at 60-70°C (for example, 60°C, 61°C, 62°C, 63°C, 64°C, 65°C, 66°C, 67°C, 68°C, 69°C or 70°C, etc.).

[0052] The numerical range of the present application includes not only the point values listed above, but also any point values between the above numerical ranges that are not listed, and the present application does not list the specific point values included in the range for the sake of brevity and simplicity.

[0053] Compared with the prior art, the present application has the following beneficial effects:

[0054] The composition with soothing function provided by the application comprises silk fibroin composite microspheres, hydrogenated lecithin, silk amino acids and glycerol glucoside and other components. First, the composition uses the characteristics of the silk fibroin composite microspheres that can be absorbed through the skin, penetrates into the skin muscle layer, and delivers skin conditioners, moisturizers and emollients; at the same time, the skin conditioner is preferably hydrogenated lecithin, silk amino acids and glycerol glucoside, which can further improve the soothing effect of the composition.

[0055] The spray prepared by using the composition can instantly and effectively provide moisture supplement for the skin, quickly relieve skin discomfort, and strengthen barrier toughness. Through experiments, it is found that after using the spray for 10 days, the erythema area and erythema index can be obviously reduced, the erythema area is reduced by 10.43%-15.82%, and the change rate of the erythema index is 12.97%-22.75%. DETAILED DESCRIPTION

[0056] The technical solutions of the application are further described below through specific embodiments, but the following examples are only simple examples of the application and do not represent or limit the protection scope of the application. The protection scope of the application is subject to the claims.

[0057] In the application, the silk fibroin can be purchased from Suzhou Suhaobio Material Co., Ltd.; the PDRN can be purchased from Regenomed Biotech Co., Ltd. or Shanghai Huivin Biotech Co., Ltd.; the polylysine can be purchased from Shandong Furuida Biotechnology Co., Ltd. or Shanghai Maikelin Biochemical Technology Co., Ltd.; the hydrogenated lecithin can be purchased from NIPPON SURFACTANT INDUSTRIES CO., LTD., and the model number is S-10; the silk amino acids can be purchased from Beijing Aosimei Biotechnology Co., Ltd., and the product name is Silkshuyuan; the glycerol glucoside can be purchased from Guangdong Xianjia Yipin Health Technology Co., Ltd.; the butanediol can be purchased from Dailch Co., Ltd.; the trehalose can be purchased from Nagase Viita Co., Ltd., and the model number is TREHALOSE 100; the glycerol can be purchased from Procter & Gamble International Operations SA Singapore Branch; the 1,2-hexanediol can be purchased from Microcare Emollient HXD(P) Thor Chemical; the hyaluronic acid can be purchased from Shandong Anhua Biomedical Co., Ltd., and the product name is ProHA hyaluronic acid; the p-hydroxyacetophenone can be purchased from Dehn, and the product name is Xinxianketone; the caprylic / capric triglyceride can be purchased from Croda Singapore Pte Ltd, and the product name is CRODAMOL GTCC-LQ-(SG); the betaine can be purchased from Texas Onelida Biotechnology Co., Ltd.

[0058] The rest of the materials not mentioned can be purchased from conventional manufacturers in the field.

[0059] The silk fibroin composite microsphere aqueous solution described in the present application can be prepared according to the following method:

[0060] (1) 1 g of PDRN (DNA content ≥70%, purchased from Regenex Biosciences Co., Ltd.) was dissolved in 50 g of water and stirred to obtain a PDRN solution; 1 g of silk fibroin (purchased from Suzhou Suhaobio Biological Material Co., Ltd.) was dissolved in 25 g of water and stirred to obtain a silk fibroin solution; and 0.05 g of polylysine (purchased from Shandong Furuida Biological Technology Co., Ltd.) was dissolved in 25 g of water and stirred.

[0061] (2) The silk fibroin solution prepared in step (1) was mixed with the PDRN solution, and stirred at a speed of 600 rpm for 60 min, and then the polylysine solution was added and stirred at a speed of 600 rpm for 30 min.

[0062] (3) 0.5% by mass of hydroxyethyl cellulose (purchased from Beijing Kangtai Long Science and Technology Development Co., Ltd., molecular weight viscosity 3400-5000 mPa.s) was added, and then citric acid was used to adjust the pH of the solution to about 6 to obtain an aqueous solution containing the silk fibroin composite microspheres, wherein the particle size of the silk fibroin composite microspheres is <500 nm.

[0063] Example 1

[0064] This example provides a spray with soothing effect, and the preparation raw materials are shown in Table 1, which include:

[0065] Table 1

[0066] No. Name Mass % content Efficacy 1 Silk fibroin composite microspheres aqueous solution 0.1 Skin protectant 2 Hydrogenated lecithin 0.12 Skin conditioning agent 3 Silk amino acids 0.1 Skin conditioning agent 4 Glycerin glucoside 0.0001 Skin conditioning agent 5 Butylene glycol 5 Moisturizing agent 6 Trehalose 0.5 Moisturizing agent 7 Glycerin 0.5 Moisturizing agent 8 1,2-hexanediol 0.5 Moisturizing agent 9 Hyaluronic acid 0.1 Moisturizing agent 10 p-Hydroxyacetophenone 0.5 Antioxidant 11 Caprylic / capric triglyceride 0.4 Emollient 12 Water Make up to 100% -

[0067] The preparation method is as follows:

[0068] (1) Pre-dissolution: p-hydroxyacetophenone and butanediol were dissolved and stirred at 60°C for standby; caprylic acid / capric acid triglyceride was dissolved and stirred at 50°C for standby;

[0069] (2) Preparation of agent solution: hydrogenated lecithin, trehalose, glycerol, and hyaluronic acid were put into an emulsifying kettle and stirred and heated to 85°C, and then cooled to 60°C after uniform stirring;

[0070] The prepared p-hydroxyacetophenone was added, and after uniform stirring, the temperature was lowered to 45°C;

[0071] Caprylic acid / capric acid triglyceride was added, and after uniform stirring, the heating was stopped;

[0072] Add the aqueous solution of fibroin complex microspheres, silk amino acids, glycerol glucoside and 1,2-hexanediol, make up the water content, and stir uniformly to obtain the composition with soothing effect.

[0073] (3) Product filling and packaging: put the valve on the jar, fill the propellant into the jar, and seal; fill the liquid into the jar through the filling machine, check for leakage in the water bath, pass the weighing machine and pressure tester, and then obtain the spray with soothing effect after being equipped with the spray head and the outer cover.

[0074] Example 2

[0075] This example provides a spray with soothing effect, which is different from Example 1 in that it does not contain glycerol glucoside, and the missing mass percentage is made up by hydrogenated lecithin.

[0076] Example 3

[0077] This example provides a spray with soothing effect, which is different from Example 1 in that it does not contain silk amino acids, and the missing mass percentage is made up by hydrogenated lecithin.

[0078] Example 4

[0079] This example provides a spray with soothing effect, which is different from Example 1 in that it does not contain hydrogenated lecithin, and the missing mass percentage is made up by silk amino acids.

[0080] Example 5

[0081] This example provides a spray with soothing effect, which is different from Example 1 in that it does not contain trehalose, and the missing mass percentage is made up by water.

[0082] Example 6

[0083] This example provides a spray with soothing effect, which is different from Example 1 in that it replaces silk amino acids with betaine.

[0084] Example 7

[0085] This example provides a spray with soothing effect, which is different from Example 1 in that it replaces hyaluronic acid with a carbohydrate isomer.

[0086] Example 8

[0087] This example provides a spray with soothing effect, which is different from Example 1 in that it replaces caprylic / capric triglyceride with isopropyl palmitate.

[0088] Example 9

[0089] This example provides a spray with soothing effect, which is different from Example 1 in that it replaces hydrogenated lecithin with tocopherol.

[0090] Comparative Example 1

[0091] This comparative example provides a spray, which is different from Example 1 in that it does not contain a silk fibroin composite microsphere aqueous solution in the raw materials for preparation, and the missing mass percentage is made up by hydrogenated lecithin.

[0092] Comparative Example 2

[0093] This comparative example provides a spray, which is different from Example 1 in that the mass percentage of the silk fibroin composite microsphere aqueous solution in the raw materials for preparation is reduced to 0.01%, and the missing mass percentage is made up by hydrogenated lecithin.

[0094] Comparative Example 3

[0095] This comparative example provides a spray, which is different from Example 1 in that it does not contain caprylic acid / capric acid triglyceride in the raw materials for preparation; the missing mass percentage is made up by the silk fibroin composite microsphere aqueous solution.

[0096] Comparative Example 4

[0097] This comparative example provides a spray, which is different from Example 1 in that it does not contain a silk fibroin composite microsphere aqueous solution in the raw materials for preparation, and the missing mass percentage is made up by adding an equal amount of silk fibroin and PDRN directly in the preparation process, rather than preparing them into composite microspheres in advance.

[0098] Test Example 1

[0099] Physicochemical and microbiological indicators

[0100] (1) Test samples: sprays prepared from Examples 1-9 and Comparative Examples 1-4.

[0101] (2) The physicochemical and microbiological indicators in the product were detected according to the Cosmetics Production Safety Technical Specifications, and the detection method referred to the Cosmetics Safety Technical Specifications (2015 edition).

[0102] Table 2

[0103] Item Standard Total number of colonies ≤1000 CFU / ml Total number of molds and yeasts ≤100 CFU / ml Thermotolerant coliforms Not detected / ml Staphylococcus aureus Not detected / ml Pseudomonas aeruginosa Not detected / ml Mercury ≤1 mg / kg Arsenic ≤2 mg / kg Lead ≤10 mg / kg Cadmium ≤5 mg / kg Methanol ≤2000 mg / kg Dioxane ≤30 mg / kg

[0104] As shown in Table 2, the test samples all meet the above technical specifications.

[0105] Test Example 2

[0106] Human skin patch test

[0107] A total of 30 people, 0 men and 30 women, aged 18-40 years, met the voluntary selection criteria for subjects.

[0108] Patch method: select qualified patch tester, with closed patch test method, about 0.025 mL of the test substance (examples 1-9 and comparative examples 1-4, as samples before filling are detected) is dropped in the patch tester, and the special adhesive tape for external use is pasted on the back of the subject, and the test substance is removed after 24 h, and the skin reaction is observed at 0.5 h, 24 h and 48 h respectively after removal.

[0109] The human skin patch test results show that 0 cases of skin adverse reactions occur in 30 people; it is proved that the cosmetic of the application is safe to use.

[0110] Test example 3

[0111] Human efficacy erythema index

[0112] Patients with sensitive skin generally have symptoms and manifestations such as skin redness, increased transdermal water loss rate, decreased keratin hydration, and sensitive skin to external stimuli.

[0113] The skin sensitivity test in the application uses the Boman skin type questionnaire (BSTQ).

[0114] The test is a human test, and the subjects are 18-60 years old, who think their skin is dry and rough, and men and women can be tested; the subjects self-evaluate, and the Boman skin type questionnaire (sensitive skin) score is ≥30 points. The subjects are randomly divided into groups, and the number of subjects for each sample is ≥30, the test method is test spray, twice a day in the morning and evening, and sprayed on one side of the face (one person can test two samples on the left and right sides of the face).

[0115] A total of 10 days are used, and during this period, no other skin care products or medical products, etc. are allowed to be used, and if any occurs, the test is immediately excluded.

[0116] The erythema area and erythema index (Erythema index, EI, the higher the index, the more serious the facial erythema) of the test subjects are tested at 0 days and 10 days, respectively, and the results are shown in Table 3.

[0117] Table 3

[0118] Sample Erythema area (change rate) Erythema index (change rate) Example 1 15.82 22.75 Example 2 11.87 17.89 Example 3 13.21 15.42 Example 4 10.43 12.97 Example 5 14.56 20.13 Example 6 12.28 18.24 Example 7 13.65 17.31 Example 8 11.92 15.58 Example 9 12.40 17.32 Comparative Example 1 2.27 6.90 Comparative Example 2 4.24 8.01 Comparative Example 3 6.75 9.16 Comparative Example 4 3.51 7.83

[0119] As shown in the above table, the spray provided by the application has good soothing effect.

[0120] Among them, the comparison of example 1 and comparative examples 1 and 4 shows that without the silk fibroin composite microspheres described in the application, the prepared spray has poor soothing effect; and combined with comparative example 2, it can be known that the content of silk fibroin composite microspheres is low, which will also significantly reduce the soothing effect of the spray.

[0121] It can be seen from the comparison of example 1 and examples 2-4, 6, 9 that the hydrogenated lecithin, silk amino acid and glycerol glucoside are synergistic with each other as the skin conditioning agent, and none of them can be omitted, and if one of them is omitted or replaced by an equivalent amount of other ingredients, the soothing effect will be reduced.

[0122] It can be seen from the comparison of example 1, examples 5, 7-8 and comparative example 3 that the emollient and humectant ingredients in the spray are essential, but the selection of the humectant and emollient is relatively wide, such as sugar class isomer, isopropyl palmitate, etc., but the soothing effect is not as obvious as the combination of the humectant and emollient provided in example 1. Therefore, the combination described in example 1 of the present application has a relatively obvious soothing effect.

[0123] Finally, it should be pointed out that: the above examples are only used to illustrate the technical solutions of the present application, but not to limit them; although the present application has been described in detail with reference to the foregoing examples, those skilled in the art should understand that: it can still modify the technical solutions recorded in the foregoing examples, or make equivalent replacement for part or all of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present application.

Claims

1. A composition having a soothing effect, characterized in that, The raw materials for preparing the composition include, by mass fraction: The composition consists of 0.05%-5% of a silk-core protein complex microsphere aqueous solution as a skin protectant, 0.2%-5% of a skin conditioning agent, 5%-10% of a moisturizer, and 0.1%-1% of a skin emollient, with the remainder being water.

2. The composition according to claim 1, characterized in that, The aqueous solution of the silk-core protein composite microspheres contains silk-core protein composite microspheres, which are prepared by the following method: silk-core protein and polydeoxyribonucleic acid are interpenetrated, entangled, folded and compressed under the action of polylysine to obtain the silk-core protein composite microspheres; Preferably, the average particle size of the silk-core protein composite microspheres is 50 nm to 500 nm; Preferably, the polylysine comprises basic polylysine and / or modified polylysine.

3. The composition according to claim 2, characterized in that, In the raw materials for preparing the silk core protein composite microspheres, the mass ratio of silk core protein to polylysine is 1:(0.006~0.2), the mass ratio of silk core protein to polydeoxyribonucleic acid is 1:(1~10), and the mass ratio of polylysine to polydeoxyribonucleic acid is 1:(5~150). Preferably, the mass percentage of silk core protein in the aqueous solution of the silk core protein composite microspheres is 1%-5%; Preferably, the mass percentage of polylysine in the aqueous solution of the silk-core protein composite microspheres is 0.01%-1%; Preferably, the mass percentage of polydeoxyribonucleic acid in the aqueous solution of the silk core protein composite microspheres is 1%-5%.

4. The composition according to claim 2, characterized in that, The aqueous solution of the silk-core protein composite microspheres also contains a pH adjuster, and the pH of the aqueous solution of the silk-core protein composite microspheres is 5.5-6.5; Preferably, the pH adjuster comprises any one or a combination of at least two of citric acid, lactic acid, malic acid, tartaric acid or glycolic acid, with citric acid being the most preferred. Preferably, the aqueous solution of the silk-core protein composite microspheres further contains a thickener, and the content of the thickener in the aqueous solution of the silk-core protein composite microspheres is 0.5%-1%; Preferably, the thickener is hydroxyethyl cellulose.

5. The composition according to claim 1, characterized in that, The skin conditioning agent includes any one or a combination of at least two of hydrogenated lecithin, silk amino acids, or glyceryl glucoside; Preferably, the moisturizer includes any one or a combination of at least two of butylene glycol, trehalose, glycerin, 1,2-hexanediol, 1,2-pentanediol, or hyaluronic acid; Preferably, the emollient is any one or a combination of at least two of the following: caprylic / capric triglyceride, isopropyl myristate, isopropyl palmitate, or dioctyl carbonate, with caprylic / capric triglyceride being the most preferred.

6. The composition according to claim 1, characterized in that, The composition also includes antioxidants; Preferably, the antioxidant has a mass percentage content of 0.1%-1%; Preferably, the antioxidant is any one or a combination of at least two of p-hydroxyacetophenone, octanoyl hydroxamic acid, tocopherol, ubiquinone, or ascorbic acid, and is preferably p-hydroxyacetophenone.

7. A spray with a soothing effect, characterized in that, The spray comprises the composition as described in any one of claims 1-6; Preferably, the spray is a binary packaged capsule valve aerosol; The sprayed material can be any one of solid, liquid or gaseous state; The spray can be in the form of mist, foam, powder, or micelles.

8. A method for preparing a spray as described in claim 7, characterized in that, The preparation method includes the following steps: Mix the formulated amount of silk protein composite microsphere aqueous solution, skin conditioning agent, moisturizer, emollient, optional antioxidant and water, stir, take samples for testing and qualified, and then fill into the spray to obtain the spray.

9. The preparation method according to claim 8, characterized in that, The preparation method of the aqueous solution of the silk core protein composite microspheres includes the following steps: Add polydeoxyribonucleic acid to water and stir until homogeneous to obtain an aqueous solution of polydeoxyribonucleic acid; Dilute the silk protein solution, mix it with an aqueous solution of polydeoxyribonucleic acid, add an aqueous solution of polylysine to obtain a composite solution system, and stir to mix. Then add an optional pH adjuster and thickener, and stir to obtain the aqueous solution of the silk core protein composite microspheres.

10. The preparation method according to claim 8, characterized in that, The humectant is pre-dissolved at 80℃-90℃; Preferably, the emollient is pre-dissolved at 40℃-60℃; Preferably, the antioxidant is pre-dissolved at 60°C-70°C.