Traditional Chinese medicine extract for promoting peripheral platelet generation and preparation thereof

Abstract

The invention relates to a traditional Chinese medicine extract for promoting peripheral thrombogenesis and a preparation thereof, in particular to a crude loropetalum chinense extract obtained by taking loropetalum chinense and loropetalum chinense as raw materials, crushing, adding water or an organic solvent as an extraction solvent, extracting, and concentrating and drying under reduced pressure; the crude extract is adsorbed by macroporous resin and eluted by ethanol with different concentrations to obtain a purified loropetalum chinense extract, and the crude loropetalum chinense extract or the purified loropetalum chinense extract can significantly increase the peripheral blood platelet level of thrombocytopenia model animals and promote peripheral platelet generation.

Description

TECHNICAL FIELD

[0001] The application belongs to the field of traditional Chinese medicine, and particularly relates to a traditional Chinese medicine extract for promoting peripheral platelet production and a preparation thereof. BACKGROUND

[0002] Loropetalum chinense, also known as Loropetalum, is a plant of Loropetalum in Hamamelidaceae. Loropetalum chinense (R. Br.) Oliver and its variety Loropetalum chinense var. rubrum are also plants of Loropetalum in Hamamelidaceae. Loropetalum is usually a shrub or a small tree, and is widely distributed in central, southern and southwestern provinces, and also has rich wild resources in China. The flowers, leaves and roots of Loropetalum can be used as medicine, and have the effects of clearing heat and resolving toxicity, astringing and hemostasis, and are commonly used for treating burns, scalds, external injuries, hemoptysis, metrorrhagia, diarrhea and gastric ulcer. At present, more than 60 compounds have been isolated from Loropetalum, including tannins, flavonoid glycosides, lignans and steroids, which have the effects of antibacterial, anti-inflammatory, antioxidant and promoting healing, and have high clinical application value and broad market prospects.

[0003] Thrombocytopenia is a refractory blood system disease, mainly including primary immune thrombocytopenia, secondary thrombocytopenia caused by diseases such as malignant tumors, systemic lupus erythematosus and chronic anemia, and drug-induced thrombocytopenia caused by chemotherapy drugs and thrombolytic drugs. The incidence of thrombocytopenia is high (5-10 per 100,000 people) in China, and patients generally have symptoms such as skin purpura, fatigue and anxiety, and may also have leukopenia, anemia and total hemoglobin reduction, and severe cases can die due to internal bleeding or intracranial hemorrhage. The etiology of thrombocytopenia is complex, and the pathogenesis has not been completely analyzed, and there is a lack of effective clinical treatment drugs. Glucocorticoids and gamma globulin are first-line drugs for thrombocytopenia, but have problems such as low efficiency, large side effects, high cost, inability to take long-term medication, easy recurrence after drug withdrawal, etc. Human recombinant thrombopoietin injection (such as Thrombocytopenia), oral thrombopoietin receptor agonists (such as Eltrombopag, Romiplostim, etc.) and rituximab are also widely used for various primary and secondary thrombocytopenia. Although such drugs can be taken long-term, the clinical efficiency is only about 60%, and also have problems such as high treatment cost, large side effects, easy drug resistance and easy recurrence after drug withdrawal.

[0004] The researches on the domestic M. alba and M. rubra mainly focus on the isolation of compounds and medicinal activities. Zhang Wugang et al. also recorded that the leaves of M. alba have the effects of “relieving blood stasis, resolving heat and stopping diarrhea, treating abdominal pain, muscle spasm, diarrhea, callus and external injury hemorrhage” (Analysis of Hemostatic Chemical Constituents of M. alba, Chinese Journal of Experimental Traditional Medical Formulas, 2017, 23(05): 47-52) and other effects. Liang Zeqin et al. reported that M. alba can promote the repair of damaged epithelial tissue and converge wound, and has significant and accurate effects of promoting hemostasis and blood coagulation (Preliminary Study on the Material Basis of M. alba for Promoting Wound Healing in Rats, Chinese Journal of Chinese Materia Medica, 2013, 38(20): 3566-3570), which is widely reported in clinical treatment of external injury hemorrhage, postpartum poor uterine contraction and hemorrhage, and promotion of tissue healing at surgical site.

[0005] Therefore, it is particularly urgent to develop a more effective traditional Chinese medicine extract for clinical treatment of thrombocytopenia. SUMMARY

[0006] The purpose of the present application is to obtain M. alba and M. rubra medicinal materials as raw materials, extract M. alba extract, and the extract has the effects of increasing peripheral platelets and promoting peripheral platelet production.

[0007] The first aspect of the present application relates to a M. alba crude extract prepared by the following method, step (1), crushing M. alba, adding water or organic solvent as an extractant; step (2), reflux extraction, vacuum concentration, to obtain M. alba crude extract.

[0008] The first aspect of the present application relates to a M. alba crude extract prepared by the following method, step (1), crushing M. alba, adding water or organic solvent as an extractant; step (2), reflux extraction, vacuum concentration, to obtain M. alba crude extract.

[0009] In the step (1), the ratio of M. alba to the extractant is 1:1-12 kg / L, and the standing time is 0.5-18 h; in the step (2), the reflux extraction is performed for 1-4 times, and the extraction time is 0.2-4 h each time.

[0010] Preferably, in the step (1), the screening is 18 meshes; the ratio of M. alba to the extractant is 1:2-10 kg / L, and the standing time is 1-12 h; in the step (2), the reflux extraction is performed for 1-3 times, and the extraction time is 0.5-2 h each time, and the extraction liquid is vacuum concentrated to a relative density of 1.10-1.30 (60℃).

[0011] The alcohol organic solvent in the step (1) is methanol, ethanol, propanol or butanol, preferably methanol or ethanol; the ester organic solvent is ethyl formate, ethyl acetate, propyl acetate, isopropyl acetate or butyl acetate, preferably ethyl acetate; and the ketone organic solvent is acetone, butanone or pentanone, preferably acetone.

[0012] Preferably, the mass fraction of the alcohol organic solvent is 5%-100%, 10%-80%, 20%-60% or 40%-60%.

[0013] Further preferably, the mass fraction of the alcohol organic solvent is 20%, 40%, 60%, 80% or 100%.

[0014] The total phenol content of the crude extract of Loropetalum chinense is 1%-99%, 5%-95%, 6%-90%, 7%-85%, 8%-80%, 9%-78%, 10%-76%, 11%-74%, 12%-70%, 13%-68%, 14%-66%, 15%-64%, 16%-62%, 18%-60%, 20%-58%, 22%-56%, 24%-54%, 26%-52%, 28%-50%, 30%-48%, 32%-46%, 34%-44%, 32%-42%, 34%-40% or 36%-38%.

[0015] Preferably, the total phenol content of the crude extract of Loropetalum chinense is 7.3%, 19.4%, 25.4%, 31.3%, 20.1%, 23.1%, 6.6%, 5.8%, 7.3%, 15.2%, 22.3%, 30.2%, 19.4%, 21.4%, 7.0%, 5.3%, 17.8%, 62.3%, 50.4%, 42.1%, 15.4%, 70.2%, 58.7%, 43.3%, 10.2%, 55.7%, 48.9%, 40.3%, 19.1%, 58.2%, 55.9% or 38.9%.

[0016] The second aspect of the present application relates to a preparation method of a crude extract of Loropetalum chinense, characterized in that: step (1) is to crush Loropetalum chinense, sieve, add water or an alcohol organic solvent or an ester organic solvent or a ketone organic solvent as an extractant, and stand still; and step (2) is to reflux extraction, combine the extract, and reduce pressure concentration to obtain the crude extract of Loropetalum chinense.

[0017] Preferably, in the step (1), the material-liquid ratio of Loropetalum chinense to the extractant is 1:1-12 kg / L, and the standing time is 0.5-18 h; and in the step (2), the reflux extraction is performed 1-4 times, and each extraction time is 0.2-4 h.

[0018] Preferably, in the step (1), the sieving is 18 mesh; the ratio of the extractant to the material is 1:2-10 kg / L, and the standing time is 1-12 h; in the step (2), the reflux extraction is performed 1-3 times, and the extraction time is 0.5-2 h each time; and the extraction liquid is concentrated under reduced pressure to a relative density of 1.10-1.30 (60°C).

[0019] Further preferably, in the step (1), the alcohol organic solvent is methanol, ethanol, propanol or butanol; the ester organic solvent is ethyl formate, ethyl acetate, propyl acetate, isopropyl acetate or butyl acetate; and the ketone organic solvent is acetone, butanone or pentanone.

[0020] Still further preferably, the mass fraction of the alcohol organic solvent is 5%-100%, 10%-80%, 20%-60% or 40%-60%; and still further preferably, the mass fraction of the alcohol organic solvent is 20%, 40%, 60%, 80% or 100%.

[0021] The purified extract of Michelia is prepared by the following method: the crude extract of Michelia according to any one of claims 1-5 is purified by elution with macroporous resin to obtain Michelia purified extract of different polarities.

[0022] The purified extract of Michelia is prepared by the following method: the crude extract of Michelia according to any one of claims 1-5 is dissolved with distilled water, adsorbed with a macroporous resin column, eluted with deionized water, discarded, eluted with alcohol organic solvents of different concentrations, collected, concentrated under reduced pressure, and dried to obtain the Michelia purified extract; and the macroporous resin column is selected from one of the following types: HPD-300, HPD-400, HPD-450, HPD-600, HPD-826, ADS-17, ADS-21, AB-8, D101 or D301.

[0023] The macroporous resin column is selected from one of the following types: AB-8, HPD-300, ADS-17 or D101; the adsorption flow rate is 1-8 mL·min -1 The macroporous resin has a diameter of 1-6 cm and a diameter-height ratio of 1:2-8, and the loading amount is 10-60 mL with a static adsorption time of 2-24 h; the deionized water elution amount is 1BV-6BV; and the alcohol organic solvent is ethanol with a mass fraction of 10%-80% and an amount of 2-10BV.

[0024] Preferably, the adsorption flow rate is 2-6 mL·min -1 The macroporous resin has a diameter of 2-4 cm and a diameter-height ratio of 1:2-4, and the loading amount is 20-40 mL with a static adsorption time of 4-24 h; the deionized water elution amount is 2-6BV; and the ethanol gradient elution mass fraction is 10%, 30%, 50% or 70% with an amount of 2-8BV.

[0025] More preferably, the adsorption flow rate is 5 mL·min -1 The macroporous resin has a diameter of 4 cm, a diameter-height ratio of 1:4 or 1:8, a loading amount of 20 mL or 40 mL, a static adsorption of 12 h, the ionized water has an elution amount of 2 BV, and the ethanol gradient elution has a mass fraction of 10%, 30%, 50% or 70% and an amount of 5 BV or 6 BV.

[0026] Preferably, the total phenol content of the purified extract of Michelia is 17.8%, 62.3%, 50.4%, 42.1%, 15.4%, 70.2%, 58.7%, 43.3%, 10.2%, 55.7%, 48.9%, 40.3%, 19.1%, 58.2% or 38.9%.

[0027] Preferably, the total phenol content of the purified extract of Michelia is 17.8%, 62.3%, 50.4%, 42.1%, 15.4%, 70.2%, 58.7%, 43.3%, 10.2%, 55.7%, 48.9%, 40.3%, 19.1%, 58.2% or 38.9%.

[0028] The third aspect of the present application relates to a preparation method of a purified extract of Michelia, characterized in that: the purified extract of Michelia is prepared by the following method, the crude extract of Michelia as claimed in any one of claims 1-5 is redissolved with distilled water, adsorbed by a macroporous resin column, eluted with deionized water, discarded, and then eluted with different concentrations of alcohol organic solvents, different concentrations of eluents are collected, concentrated under reduced pressure, and dried to obtain the purified extract of Michelia; the macroporous resin column is selected from one of the following types: HPD-300, HPD-400, HPD-450, HPD-600, HPD-826, ADS-17, ADS-21, AB-8, D101 or D301.

[0029] The macroporous resin column is selected from one of the following types: AB-8, HPD-300, ADS-17 or D101; the adsorption flow rate is 1-8 mL·min -1 The macroporous resin has a diameter of 4 cm, a diameter-height ratio of 1:4 or 1:8, a loading amount of 20 mL or 40 mL, a static adsorption of 12 h, the ionized water has an elution amount of 2 BV, and the ethanol gradient elution has a mass fraction of 10%, 30%, 50% or 70% and an amount of 5 BV or 6 BV.

[0030] Preferably, the adsorption flow rate is 2-6 mL·min -1 Preferably, the macroporous resin has a diameter of 2-4 cm, a diameter-height ratio of 1:2-4, a loading amount of 20-40 mL, and a static adsorption time of 4-24 h; the ionized water elution amount is 2-6 BV; the ethanol gradient elution mass fraction is 10%, 30%, 50%, or 70%, and the amount is 2-8 BV.

[0031] More preferably, the adsorption flow rate is 5 mL·min -1 Preferably, the macroporous resin has a diameter of 4 cm, a diameter-height ratio of 1:4 or 1:8, a loading amount of 20 mL or 40 mL, a static adsorption time of 12 h, an ionized water elution amount of 2 BV, and an ethanol gradient elution mass fraction of 10%, 30%, 50%, or 70%, and the amount is 5 BV or 6 BV.

[0032] The fourth aspect of the present application relates to a traditional Chinese medicine preparation, characterized in that it comprises the crude extract of Loropetalum chinense or the purified extract of Loropetalum chinense and a pharmaceutically acceptable excipient.

[0033] The traditional Chinese medicine preparation is selected from the group consisting of tablets, capsules, buccal preparations, granules, pills, instant preparations, powders, pastes, boluses, suspensions, solutions, injections, suppositories, pastes, sprays, drops, drop pills, and patches.

[0034] The following is an explanation and description of the terms of the present application:

[0035] The information related to Loropetalum chinense is as follows: Loropetalum chinense (R. Brown) Oliv. is a plant of Hamamelidaceae Hamamelis genus. It has the effects of astringency, hemostasis, heat-clearing and detoxification, and diarrhea-stopping. It is used for treating various bleeding syndromes such as epistaxis, hemoptysis, trauma, and so on, as well as scalding, diarrhea, and dysentery.

[0036] The term "pharmaceutically acceptable" refers to a carrier, vehicle, diluent, excipient, and / or formed salt that is generally chemically or physically compatible with other ingredients constituting a pharmaceutical dosage form, and is physiologically compatible with a subject.

[0037] The pharmaceutical composition of the present application can be in any one of the reusable pharmaceutical preparation forms, such as oral, injection, external use and the like, the oral dosage forms include but are not limited to tablets, capsules, oral liquids, granules, pills, suspensions, injection preparations selected from water injection, powder injection, external preparation selected from patches, ointments. All preparations can be prepared according to the conventional techniques of pharmacy, such as taking any one of the compounds of the present application, or its stereoisomer, or its pharmaceutically acceptable salt as the pharmaceutical active ingredient, if necessary, adding a pharmaceutically acceptable carrier, and preparing the above-mentioned pharmaceutical dosage forms suitable for administration, wherein the unit dose of the pharmaceutical active ingredient can be 0.1 mg-1000 mg, such as 0.1 mg-1000 mg of the pharmaceutical active ingredient per tablet, preferably 5-500 mg.

[0038] The dosage of the extract and the effective component thereof of the present application depends on the age, weight and disease type and severity of the subject.

[0039] Obviously, according to the above content of the present application, according to the ordinary technical knowledge and common means in the art, other various forms of modification, replacement or change can be made without departing from the above-mentioned basic technical idea of the present application.

[0040] The beneficial technical effects of the present application are:

[0041] 1. It is generally believed that the promotion of platelet production mainly depends on the stimulation of bone marrow megakaryocyte differentiation and maturation, the promotion of platelet production and the reduction of megakaryocyte and platelet destruction. The present application finds that the extract of E. acuminatum obtained by a certain extraction and purification process can significantly increase the peripheral blood platelet level of a platelet reduction model animal and promote the peripheral blood platelet production. Unlike the body's mechanism of hemostasis and coagulation through a series of mechanisms such as contraction of blood vessel wall, stimulation of platelet aggregation, activation of coagulation factors and fibrinolysis system.

[0042] 2. The present application provides the use of E. acuminatum and E. indica extract in promoting peripheral blood platelet production. Using a platelet reduction mouse model caused by a chemotherapeutic drug, the extract and purified components of E. acuminatum and E. indica prepared by the extraction method of the present application can significantly promote the peripheral blood platelet production of the model mice. The use of E. acuminatum and E. indica extract has not been reported in the prior art. DETAILED DESCRIPTION

[0043] The present application is further illustrated by the following examples, but is not limited thereto.

[0044] Example 1: Preparation of E. acuminatum crude extract and determination of total phenol content

[0045] (1) 500 g of fresh E. acuminatum material was crushed and then passed through an 18-mesh sieve, respectively;

[0046] Then add water as extraction solvent to the powder, which is recorded as No. 1 crude extract of M. alba (abbreviated as white crude 1) ;

[0047] Add 20% ethanol as extraction solvent, which is recorded as No. 2 crude extract of M. alba (abbreviated as white crude 2) ;

[0048] Add 40% ethanol as extraction solvent, which is recorded as No. 3 crude extract of M. alba (abbreviated as white crude 3) ;

[0049] Add 60% ethanol as extraction solvent, which is recorded as No. 4 crude extract of M. alba (abbreviated as white crude 4) ;

[0050] Add 80% ethanol as extraction solvent, which is recorded as No. 5 crude extract of M. alba (abbreviated as white crude 5) ;

[0051] Add 100% methanol as extraction solvent, which is recorded as No. 6 crude extract of M. alba (abbreviated as white crude 6) ;

[0052] Add acetone as extraction solvent, which is recorded as No. 7 crude extract of M. alba (abbreviated as white crude 7) ;

[0053] Add ethyl acetate as extraction solvent, which is recorded as No. 8 crude extract of M. alba (abbreviated as white crude 8) ;

[0054] The ratio of each group of liquid to material is 1:2 Kg / L, and the mixture is allowed to stand for 12 hours, then continuously condensed and refluxed for 3 times, 0.5 hours each time, the extract is combined and concentrated under reduced pressure to a relative density of 1.10-1.30 (60℃), to obtain the following crude extracts of M. alba: No. 1 crude extract of M. alba, No. 2 crude extract of M. alba, No. 3 crude extract of M. alba, No. 4 crude extract of M. alba, No. 5 crude extract of M. alba, No. 6 crude extract of M. alba, No. 7 crude extract of M. alba and No. 8 crude extract of M. alba.

[0055] (2) 500g of fresh M. alba material is crushed and then passed through an 18 mesh sieve;

[0056] Then add water as extraction solvent to the powder, which is recorded as No. 9 crude extract of M. alba (abbreviated as white crude 9) ;

[0057] Add 20% ethanol as extraction solvent, which is recorded as No. 10 crude extract of M. alba (abbreviated as white crude 10) ;

[0058] Add 40% ethanol as extraction solvent, which is recorded as No. 11 crude extract of M. alba (abbreviated as white crude 11) ;

[0059] Add 60% ethanol as extraction solvent, which is recorded as No. 12 crude extract of M. alba (abbreviated as white crude 12) ;

[0060] Add 80% ethanol as extraction solvent, which is recorded as No. 13 crude extract of M. alba (abbreviated as white crude 13) ;

[0061] Add 100% methanol as the extraction solvent, and record as No. 14 crude extract of E. acuminatum (abbreviated as Bai crude 14);

[0062] Add acetone as the extraction solvent, and record as No. 15 crude extract of E. acuminatum (abbreviated as Bai crude 15);

[0063] Add ethyl acetate as the extraction solvent, and record as No. 16 crude extract of E. acuminatum (abbreviated as Bai crude 16);

[0064] Each group of material liquid ratio is 1:10, and then continuously condense reflux extraction for 1 hour, each time for 2 hours, and then combine the extract and reduce pressure concentration to relative density of 1.10-1.30 (60℃); The following crude extracts of E. acuminatum are obtained: No. 9 crude extract of E. acuminatum, No. 10 crude extract of E. acuminatum, No. 11 crude extract of E. acuminatum, No. 12 crude extract of E. acuminatum, No. 13 crude extract of E. acuminatum, No. 14 crude extract of E. acuminatum, No. 15 crude extract of E. acuminatum and No. 16 crude extract of E. acuminatum.

[0065] (3) The total phenol content of each crude extract of E. acuminatum is detected by Folin phenol colorimetry with gallic acid as the standard. The yield and total phenol content of the crude extract of E. acuminatum are calculated according to the following formula. The total phenol content of the crude extract of E. acuminatum is shown in Table 1.

[0066] Yield of crude extract of E. acuminatum % = dry extract amount of crude extract of E. acuminatum / E. acuminatum feeding amount * 100%

[0067] Total phenol content of crude extract of E. acuminatum % = total phenol mass of crude extract of E. acuminatum / dry extract amount of crude extract of E. acuminatum * 100%

[0068] Table 1 Yield and total phenol content of crude extract of E. acuminatum prepared by different extraction processes

[0069]

[0070]

[0071] Conclusion: The yield of crude extract of E. acuminatum is between 11.5% and 65.8%, and the total phenol content is between 5.7% and 30.8%. The total phenol content of No. 4 crude extract of E. acuminatum is the highest.

[0072] Example 2 Preparation of crude extract of E. indica and determination of total phenol content

[0073] (1) 500g of fresh E. indica material is crushed, and then passed through an 18-mesh sieve;

[0074] Then add water as the extraction solvent to the powder, and record as No. 1 crude extract of E. indica (abbreviated as Hong crude 1);

[0075] 20% ethanol was added as the extraction solvent, and it was recorded as No. 2 of the crude extract of E. luteum (abbreviated as red crude 2);

[0076] 40% ethanol was added as the extraction solvent, and it was recorded as No. 3 of the crude extract of E. luteum (abbreviated as red crude 3);

[0077] 60% ethanol was added as the extraction solvent, and it was recorded as No. 4 of the crude extract of E. luteum (abbreviated as red crude 4);

[0078] 80% ethanol was added as the extraction solvent, and it was recorded as No. 5 of the crude extract of E. luteum (abbreviated as red crude 5);

[0079] Methanol was added as the extraction solvent, and it was recorded as No. 6 of the crude extract of E. luteum (abbreviated as red crude 6);

[0080] Acetone was added as the extraction solvent, and it was recorded as No. 7 of the crude extract of E. luteum (abbreviated as red crude 7);

[0081] Ethyl acetate was added as the extraction solvent, and it was recorded as No. 8 of the crude extract of E. luteum (abbreviated as red crude 8).

[0082] The ratio of each group of material liquid was 1:2, and it was left for 12 hours, and then it was continuously condensed and refluxed for 3 times, 1 hour each time, the extraction liquid was combined, and it was concentrated under reduced pressure, and the following crude extracts of E. luteum were obtained: No. 1 of the crude extract of E. luteum, No. 2 of the crude extract of E. luteum, No. 3 of the crude extract of E. luteum, No. 4 of the crude extract of E. luteum, No. 5 of the crude extract of E. luteum, No. 6 of the crude extract of E. luteum, No. 7 of the crude extract of E. luteum, and No. 8 of the crude extract of E. luteum.

[0083] (2) 500g of fresh E. luteum material was crushed, and then passed through an 18-mesh sieve;

[0084] Then water was added as the extraction solvent, and it was recorded as No. 9 of the crude extract of E. luteum (abbreviated as red crude 9);

[0085] 20% ethanol was added as the extraction solvent, and it was recorded as No. 10 of the crude extract of E. luteum (abbreviated as red crude 10);

[0086] 40% ethanol was added as the extraction solvent, and it was recorded as No. 11 of the crude extract of E. luteum (abbreviated as red crude 11);

[0087] 60% ethanol was added as the extraction solvent, and it was recorded as No. 12 of the crude extract of E. luteum (abbreviated as red crude 12);

[0088] 80% ethanol was added as the extraction solvent, and it was recorded as No. 13 of the crude extract of E. luteum (abbreviated as red crude 13);

[0089] Methanol was added as the extraction solvent, and it was recorded as No. 14 of the crude extract of E. luteum (abbreviated as red crude 14);

[0090] Add acetone as the extraction solvent, and mark as No. 15 of crude extract of E. indica (abbreviated as red crude 15);

[0091] Add ethyl acetate as the extraction solvent, and mark as No. 16 of crude extract of E. indica (abbreviated as red crude 16).

[0092] The ratio of each group of liquid to liquid is 1:12, and the extraction is carried out once for 1 hour, and then continuously condensed for 2 hours. The extraction liquid is concentrated under reduced pressure to a relative density of 1.10-1.30 (60°C), and the following crude extracts of E. indica are obtained: No. 9 of crude extract of E. indica, No. 10 of crude extract of E. indica, No. 11 of crude extract of E. indica, No. 12 of crude extract of E. indica, No. 13 of crude extract of E. indica, No. 14 of crude extract of E. indica, No. 15 of crude extract of E. indica and No. 16 of crude extract of E. indica.

[0093] (3) The total phenol content of each crude extract of E. indica is detected by Folin phenol colorimetry with gallic acid as the standard. The yield and total phenol content of the crude extract of E. indica are calculated according to the following formula. The total phenol content of the crude extract of E. indica is shown in Table 2.

[0094] The yield of the crude extract of E. indica is calculated according to the following formula:

[0095] The total phenol content of the crude extract of E. indica is calculated according to the following formula:

[0096] Table 2 The yield and total phenol content of the crude extract of E. indica prepared by different extraction processes

[0097] Group % Yield Total phenol content Group % Yield Total phenol content Red coarse 1 25.4 7.3 Red coarse 9 21.5 7.3 Red coarse 2 37.9 19.4 Red coarse 10 30.7 15.2 Red coarse 3 45.5 25.4 Red coarse 11 34.1 22.3 Red coarse 4 60.2 31.3 Red coarse 12 45.9 30.2 Red coarse 5 72.9 20.1 Red coarse 13 58.7 19.4 Red coarse 6 50.2 23.1 Red coarse 14 37.2 21.4 Red coarse 7 26.8 6.6 Red coarse 15 23.7 7.0 Red coarse 8 10.3 5.8 Red coarse 16 10.2 5.3

[0098] Conclusion: The yield of the crude extract of E. indica is between 10.3% and 72.9%, and the total phenol content is between 5.3% and 31.3%. The total phenol content of the crude extract of E. indica No. 4 is the highest.

[0099] Example 3, Preparation of purified extract components of E. indica and determination of total phenol content

[0100] (1) The extract white crude 4 in Example 1 is redissolved with distilled water, and adsorbed on a macroporous resin column at a flow rate of 5 mL·min -1 The type of macroporous adsorption resin used is AB-8, with a diameter of 4 cm and a diameter-height ratio of 1:4. The total amount of extract loaded is 20 mL, and the static adsorption time is 12 hours. First, the extract is eluted with deionized water, and the amount of distilled water used is 2 BV. The distilled water part is discarded.

[0101] Elution with 10% ethanol, 10% ethanol dosage is 5BV, the collection of this component is recorded as white Lau Lau purified extract component 1 (referred to as white pure 1) ;

[0102] Elution with 30% ethanol, 30% ethanol dosage is 6BV, the collection of this component is recorded as white Lau Lau purified extract component 2 (referred to as white pure 2) ;

[0103] Elution with 50% ethanol, 50% ethanol dosage is 6BV, the collection of this component is recorded as white Lau Lau purified extract component 3 (referred to as white pure 3) ;

[0104] Elution with 70% ethanol, 70% ethanol dosage is 5BV, the collection of this component is recorded as white Lau Lau purified extract component 4 (referred to as white pure 4).

[0105] (2) The extract white crude 4 in example 1 is selected and redissolved with distilled water, and is adsorbed through a macroporous resin column at a flow rate of 5mL·min-1. The selected macroporous adsorption resin is HPD-300, the diameter is 4cm, the diameter height ratio is 1:8, the total sample amount of the extract is 40mL, the static adsorption is 12h, and first, distilled water is eluted, and the distilled water part is discarded.

[0106] Then elution with 10% ethanol, 10% ethanol dosage is 5BV, the collection of this component is recorded as white Lau Lau purified component 5 (referred to as white pure 5) ;

[0107] Elution with 30% ethanol, 30% ethanol dosage is 6BV, the collection of this component is recorded as white Lau Lau purified component 6 (referred to as white pure 6) ;

[0108] Elution with 50% ethanol, 50% ethanol dosage is 6BV, the collection of this component is recorded as white Lau Lau purified component 7 (referred to as white pure 7) ;

[0109] Elution with 70% ethanol, 70% ethanol dosage is 5BV, the collection of this component is recorded as white Lau Lau purified component 8 (referred to as white pure 8).

[0110] (3) The extract white crude 4 in example 1 is selected and redissolved with distilled water, and is adsorbed through a macroporous resin column at a flow rate of 5mL·min -1 -1, the selected macroporous adsorption resin is ADS-17, the diameter is 4cm, the diameter height ratio is 1:4, the total sample amount of the extract is 20mL, the static adsorption is 12h, and first, distilled water is eluted, and the distilled water part is discarded,

[0111] Then elution with 10% ethanol, 10% ethanol dosage is 5BV, the collection of this component is recorded as white Lau Lau purified component 9 (referred to as white pure 9) ;

[0112] The elution was performed with 30% ethanol, the amount of 30% ethanol was 6 BV, and the component was collected and recorded as M. alba purified component 10 (abbreviated as white pure 10);

[0113] The elution was performed with 50% ethanol, the amount of 50% ethanol was 6 BV, and the component was collected and recorded as M. alba purified component 11 (abbreviated as white pure 11);

[0114] The elution was performed with 70% ethanol, the amount of 70% ethanol was 5 BV, and the component was collected and recorded as M. alba purified component 12 (abbreviated as white pure 12).

[0115] (4) The extract white crude 4 in Example 1 was redissolved with distilled water, adsorbed on a macroporous resin column at a flow rate of 5 mL·min-1, the macroporous adsorption resin used was D101, the diameter was 4 em, the diameter-height ratio was 1:8, the total sample amount of the extract was 40 mL, static adsorption was performed for 12 h, and the elution was performed with deionized water, the amount of distilled water was 2 BV, the distilled water part was discarded,

[0116] The elution was performed with 10% ethanol, the amount of 10% ethanol was 5 BV, and the component was collected and recorded as M. alba purified 13 (abbreviated as white pure 13);

[0117] The elution was performed with 30% ethanol, the amount of 30% ethanol was 6 BV, and the component was collected and recorded as M. alba purified 14 (abbreviated as white pure 14);

[0118] The elution was performed with 50% ethanol, the amount of 50% ethanol was 6 BV, and the component was collected and recorded as M. alba purified component 15 (abbreviated as white pure 15);

[0119] The elution was performed with 70% ethanol, the amount of 70% ethanol was 5 BV, and the component was collected and recorded as M. alba purified component 16 (abbreviated as white pure 16).

[0120] (5) Then, each of the M. alba purified components 1-16 was concentrated under reduced pressure, dried, and M. alba purified components were successfully prepared. The total phenol content of each M. alba purified component was detected by Folin-phenol colorimetry with gallic acid as a standard. The total phenol content of each M. alba purified component was calculated based on gallic acid, as shown in Table 3.

[0121] (6) The total phenol content of each M. alba purified component was detected by Folin-phenol colorimetry with gallic acid as a standard. The transfer rate and total phenol content of each M. alba purified component were calculated according to the following formula, as shown in Table 3.

[0122] M. alba purified component transfer rate % = M. alba purified component total phenol mass / M. alba crude extract total phenol mass * 100%

[0123] The total phenol content of the purified M. alba is calculated as follows: total phenol content of the purified M. alba (%) = (mass of total phenol in the purified M. alba) / (mass of dry extract of the purified M. alba) * 100%

[0124] Table 3. Transfer rate and total phenol content of the purified M. alba components prepared by different purification processes

[0125] Group % Transfer Total phenol content Group % Transfer Total phenol content White pure 1 29.9 17.8 White pure 9 40.3 10.2 White pure 2 36.5 62.3 White pure 10 55.1 55.7 White pure 3 43.8 50.4 White pure 11 69.3 48.9 White pure 4 47.7 42.1 White pure 12 74.8 40.3 White pure 5 52.1 15.4 White pure 13 42.7 19.1 White pure 6 62.0 70.2 White pure 14 59.3 58.2 White pure 7 77.2 58.7 White pure 15 72.1 55.9 White pure 8 85.8 43.3 White pure 16 79.5 38.9

[0126] Conclusion: The transfer rate of the purified M. alba components prepared in this example ranges from 29.9% to 85.8%, and the total phenol content ranges from 10.2% to 70.2%.

[0127] Example 4. Preparation of the purified M. lamiifolia components and determination of the total phenol content

[0128] (1) The M. lamiifolia extract of Example 2 was redissolved with distilled water, and then adsorbed on a macroporous resin column at a flow rate of 2 mL·min-1. The type of the macroporous adsorption resin used was HPD-300, the diameter was 4 cm, the ratio of diameter to height was 1:4, the total amount of the extract to be loaded was 20 mL, and the static adsorption time was 12 h. First, the extract was eluted with deionized water, and the amount of the distilled water used was 2 BV. The distilled water fraction was discarded.

[0129] Then, the extract was eluted with 10% ethanol, and the amount of the 10% ethanol used was 5 BV. The fraction was collected and was designated as the purified M. lamiifolia component No. 1 (abbreviated as red pure 1).

[0130] Then, the extract was eluted with 30% ethanol, and the amount of the 30% ethanol used was 6 BV. The fraction was collected and was designated as the purified M. lamiifolia component No. 2 (abbreviated as red pure 2).

[0131] Then, the extract was eluted with 50% ethanol, and the amount of the 50% ethanol used was 6 BV. The fraction was collected and was designated as the purified M. lamiifolia component No. 3 (abbreviated as red pure 3).

[0132] Then, the extract was eluted with 70% ethanol, and the amount of the 70% ethanol used was 5 BV. The fraction was collected and was designated as the purified M. lamiifolia component No. 4 (abbreviated as red pure 4).

[0133] (2) The M. lamiifolia extract of Example 2 was redissolved with distilled water, and then adsorbed on a macroporous resin column at a flow rate of 2 mL·min-1. The type of the macroporous adsorption resin used was HPD-400, the diameter was 4 cm, the ratio of diameter to height was 1:8, the total amount of the extract to be loaded was 40 mL, and the static adsorption time was 12 h. First, the extract was eluted with deionized water, and the amount of the distilled water used was 2 BV. The distilled water fraction was discarded.

[0134] Then, the extract was eluted with 10% ethanol, and the amount of the 10% ethanol used was 5 BV. The fraction was collected and was designated as the purified M. lamiifolia component No. 5 (abbreviated as red pure 5).

[0135] Eluted with 30% ethanol, the amount of 30% ethanol was 6BV, and the component was collected and recorded as No. 6 purified component of L. indica (abbreviated as red pure 6) ;

[0136] Eluted with 50% ethanol, the amount of 50% ethanol was 6BV, and the component was collected and recorded as No. 7 purified component of L. indica (abbreviated as red pure 7) ;

[0137] Eluted with 70% ethanol, the amount of 70% ethanol was 5BV, and the component was collected and recorded as No. 8 purified component of L. indica (abbreviated as red pure 8).

[0138] (3) The red crude 4 extract in Example 2 was redissolved with distilled water, and was adsorbed by a macroporous resin column at a flow rate of 2mL·min-1. The type of macroporous adsorption resin selected was D101, the diameter was 4cm, the diameter-height ratio was 1:4, the total amount of extract solution was 20mL, and the static adsorption time was 12h. First, distilled water was used for elution, and the amount of distilled water was 2BV, and the distilled water part was discarded.

[0139] Then 10% ethanol was used for elution, the amount of 10% ethanol was 5BV, and the component was collected and recorded as No. 9 purified component of L. indica (abbreviated as red pure 9) ;

[0140] Eluted with 30% ethanol, the amount of 30% ethanol was 6BV, and the component was collected and recorded as No. 10 purified component of L. indica (abbreviated as red pure 10) ;

[0141] Eluted with 50% ethanol, the amount of 50% ethanol was 6BV, and the component was collected and recorded as No. 11 purified component of L. indica (abbreviated as red pure 11) ;

[0142] Eluted with 70% ethanol, the amount of 70% ethanol was 5BV, and the component was collected and recorded as No. 12 purified component of L. indica (abbreviated as red pure 12).

[0143] (4) The red crude 4 extract in Example 2 was redissolved with distilled water, and was adsorbed by a macroporous resin column at a flow rate of 2mL·min-1. The type of macroporous adsorption resin selected was D301, the diameter was 4cm, the diameter-height ratio was 1:8, the total amount of extract solution was 40mL, and the static adsorption time was 12h. First, distilled water was used for elution, and the amount of distilled water was 2BV, and the distilled water part was discarded.

[0144] Then 10% ethanol was used for elution, the amount of 10% ethanol was 5BV, and the component was collected and recorded as No. 13 purified component of L. indica (abbreviated as red pure 13) ;

[0145] Eluted with 30% ethanol, the amount of 30% ethanol was 6BV, and the component was collected and recorded as No. 14 purified component of L. indica (abbreviated as red pure 14, hereinafter the same) ;

[0146] The elution was performed with 50% ethanol, and the amount of 50% ethanol was 6 BV. The component was collected and was named as L. formosum purified component No. 15 (referred to as red pure 15 hereinafter).

[0147] The elution was performed with 70% ethanol, and the amount of 70% ethanol was 5 BV. The component was collected and was named as L. formosum purified component No. 16 (referred to as red pure 16 hereinafter).

[0148] (5) Then, each of the L. formosum purified components 1 to 16 was concentrated and dried, and L. formosum purified components were successfully prepared.

[0149] (6) The total phenol content of each L. formosum purified component was detected by Folin phenol colorimetry with gallic acid as a standard. The transfer rate and the total phenol content of the L. formosum purified components were calculated according to the following formula, as shown in Table 4.

[0150] L. formosum purified component transfer rate % = total phenol mass of L. formosum purified component / total phenol mass of L. formosum crude extract * 100%

[0151] L. formosum purified component total phenol content % = total phenol mass of L. formosum purified component / dry extract mass of L. formosum purified component * 100%

[0152] Table 4. Transfer rate and total phenol content of L. formosum purified components prepared by different purification processes

[0153]

[0154]

[0155] Conclusion: The L. formosum purified components prepared in this example had a transfer rate ranging from 38.7% to 84.2% and a total phenol content ranging from 10.1% to 67.1%.

[0156] Example 5 Preparation of granules

[0157] Take any one of the extract components 1100 g prepared in Examples 1-4, add an appropriate amount of lactose, mannitol and a little microcrystalline cellulose, mix thoroughly, spray into 90% ethanol solution, dry at 60°C to prepare 1500 g of granules, which are granules.

[0158] Example 6 Preparation of tablets

[0159] Take any one of the extract components 100 g prepared in Examples 1-4, liquid paraffin 10 g, talc 25 g, starch 30 g, and tartaric acid 25 g to prepare 1000 tablets by tabletting.

[0160] Example 7 Preparation of capsules

[0161] The capsule of the present embodiment is composed of a medicine liquid and a gel liquid. Take 100 g of any one of the extracts in Examples 1-4, add 10 g of vitamin E as an antioxidant and 10 g of Tween 80 as an emulsifier to prepare a medicine liquid. Take gelatin, purified water, glycerol and a preservative in a mass ratio of 1:2.5:1:5, successively add glycerol, purified water and the preservative into a gel-making tank, heat to 80°C, then add gelatin while continuously stirring and vacuumizing until the gelatin is completely dissolved, filter the gel liquid, and store the filtrate at 60°C.

[0162] Pharmacodynamic Example 1 Promoting Effect of Different Purified Components of Delonix regia and Delonix elata on Platelet Production in a Chemotherapy Drug-induced Thrombocytopenia Model

[0163] Test Purpose: To observe the effect of orally administered different purified components of Delonix regia and Delonix elata on the peripheral blood of a chemotherapy drug-induced thrombocytopenia model in mice, in order to prove their promoting effect on platelet production.

[0164] Test Substance: Select white crude 1, white crude 6, white crude 7, white crude 8, red crude 1, red crude 6, red crude 7, red crude 8 in Examples 1 and 2, and white pure 2, white pure 3, white pure 6, white pure 7, white pure 10, white pure 11, white pure 14, white pure 15, red pure 2, red pure 3, red pure 6, red pure 7, red pure 10, red pure 11, red pure 14, red pure 15 in Examples 3 and 4, respectively, dissolve them in distilled water to prepare oral preparations. In addition, we also prepared Delonix regia extract according to the preparation method in the literature and dissolved it in distilled water to prepare an oral preparation [Zhang Wugang et al. Hemostatic Chemical Constituent Analysis of Delonix regia [J]. Chinese Journal of Experimental Prescriptions] as a literature control group.

[0165] Test method: 162 C57BL / 6 mice, half male and half female. Randomly divided according to body weight: normal control group, model group, literature control group, Bai 1 crude drug group, Bai 6 crude drug group, Bai 7 crude drug group, Bai 8 crude drug group, Hong 1 crude drug group, Hong 6 crude drug group, Hong 7 crude drug group, Hong 8 crude drug group, Bai 2 pure drug group, Bai 3 pure drug group, Bai 6 pure drug group, Bai 7 pure drug group, Bai 10 pure drug group, Bai 11 pure drug group, Bai 14 pure drug group, Bai 15 pure drug group, Hong 2 pure drug group, Hong 3 pure drug group, Hong 6 pure drug group, Hong 7 pure drug group, Hong 10 pure drug group, Hong 11 pure drug group, Hong 14 pure drug group, Hong 15 pure drug group, a total of 27 groups, 6 animals in each group. Except for the normal control group, each group of mice was intraperitoneally injected with 100 mg / kg cytarabine once a day for 2 days, and from the 3rd day, intraperitoneally injected with 50 mg / kg cytarabine once a day for 3 days, and the total modeling time was 5 days. Each group was given corresponding drugs by gavage according to body weight, wherein each pure compound group was given a dose of 250 mg / kg, and each crude extract group was given a dose of 2000 mg / kg, once a day for 10 days. On the 10th day after modeling, 200 μL of blood was collected submandibularly, and the blood routine index changes were detected using a blood cell instrument.

[0166] Table 5. Platelet level changes of each group of mice after 10 days of modeling

[0167] Group Platelet level (10^9 / L) Group Platelet level (10^9 / L) Negative control group 1243.4±131.5(***) White pure 7 925.5±69.5(***) Model control group 500.5±53.4 White pure 10 878.9±203.1(**) Document control group 615.5±78.2(*) White pure 11 852.2±113.4(**) White coarse 1 755.1±109.1(**) White pure 14 900.6±81.2(***) White coarse 6 840.9±78.4(**) White pure 15 879.6±78.3(**) White coarse 7 899.1±82.8(***) Red pure 2 986.5±120.2(***) White coarse 8 612.3±61.5(*) Red pure 3 899.1±110.2(**) Red coarse 1 658.5±52.4(**) Red pure 6 999.6±72.1(***) Red coarse 6 813.5±81.2(***) Red pure 7 945.3±154.3(**) Red coarse 7 699.4±12.3(**) Red pure 10 943.7±110.1(***) Red coarse 8 603.3±41.9(*) Red pure 11 838.3±73.4(**) White pure 2 988.3±35.3(***) Red pure 14 952.4±75.5(***) White pure 3 875.5±204.0(**) Red pure 15 852.4±121.2(***) White pure 6 1047.3±165.1(***)

[0168] Note: represents no significant difference (p>0.05) compared with the model control group; * represents p<0.05 compared with the model control group; ** represents p<0.01 compared with the model control group; *** represents p<0.001 compared with the model control group; * represents p<0.05 compared with the model control group; ** represents p<0.01 compared with the model control group; *** represents p<0.001 compared with the model control group;

[0169] Test results: On the 10th day after intraperitoneal injection of cytarabine in mice, the peripheral platelet count of the model group mice decreased significantly compared with that of normal mice, indicating that the chemotherapy drug-induced thrombocytopenia model was successfully constructed. Each pure compound group of Radermachia hainanensis and Radermachia rubiginosa showed a significant effect of improving the peripheral blood platelet level of mice compared with the model group, and compared with the peripheral platelet count of the literature control group, the prepared pure compound of the application had a more significant effect, especially the group with relatively high total phenol content, which showed the most significant effect of promoting peripheral blood platelet production. In addition, each crude extract of Radermachia hainanensis and Radermachia rubiginosa also had a certain effect of increasing platelets, but required a higher dose. The results are shown in Table 5. The prepared pure compound of Radermachia hainanensis and Radermachia rubiginosa has a significant effect of promoting peripheral blood platelet production, and the effect is positively correlated with the total phenol content.

[0170] The above description is only the preferred embodiment of the present application, but the protection scope of the present application is not limited to this. Any person skilled in the art can easily think of changes or replacements within the technical range disclosed by the present application, which should be covered in the protection scope of the present application. Therefore, the protection scope of the present application should be subject to the protection scope of the claims.

Claims

1. A crude extract of Lindera aggregata, characterized in that The method comprises the following steps: (1) crushing and sieving the wampee, and adding water or an organic solvent as an extractant and standing; (2) refluxing and extracting, and combining the extract and reducing pressure to concentrate, thereby obtaining the wampee crude extract.

2. The crude extract of Loropetalum chinense according to claim 1, characterized in that The method comprises the following steps: (1) crushing and sieving the wampee, and adding water or an organic solvent as an extractant and standing; (2) refluxing and extracting, and combining the extract and reducing pressure to concentrate, thereby obtaining the wampee crude extract.

3. The crude extract of Loropetalum chinense according to claim 2, characterized in that, In the step (1), the ratio of the wampee to the extractant is 1:1-12 kg / L, and the standing time is 0.5-18 h; in the step (2), the refluxing and extracting is performed for 1-4 times, and the extracting time is 0.2-4 h each time. Preferably, in the step (1), the sieving is performed at 18 meshes, the ratio of the wampee to the extractant is 1:2-10 kg / L, the standing time is 1-12 h, the refluxing and extracting is performed for 1-3 times, and the extracting time is 0.5-2 h each time; the extract is reduced pressure to concentrate to a relative density of 1.10-1.30 (60℃).

4. The crude extract of Lindera benzoin according to claim 2, characterized in that, In the step (1), the alcohol organic solvent is methanol, ethanol, propanol or butanol, preferably methanol or ethanol; the ester organic solvent is ethyl formate, ethyl acetate, propyl acetate, isopropyl acetate or butyl acetate, preferably ethyl acetate; and the ketone organic solvent is acetone, butanone or pentanone, preferably acetone. Preferably, the mass fraction of the alcohol organic solvent is 5%-100%, 10%-80%, 20%-60% or 40%-60%. Further preferably, the mass fraction of the alcohol organic solvent is 20%, 40%, 60%, 80% or 100%.

5. The crude extract of Loropetalum chinense according to any one of claims 1 to 4, characterized in that, The total phenol content of the wampee crude extract ranges from 1%-99%, 5%-95%, 6%-90%, 7%-85%, 8%-80%, 9%-78%, 10%-76%, 11%-74%, 12%-70%, 13%-68%, 14%-66%, 15%-64%, 16%-62%, 18%-60%, 20%-58%, 22%-56%, 24%-54%, 26%-52%, 28%-50%, 30%-48%, 32%-46%, 34%-44%, 32%-42%, 34%-40% or 36%-38%. Preferably, the total phenol content of the wampee crude extract is 7.3%, 19.4%, 25.4%, 31.3%, 20.1%, 23.1%, 6.6%, 5.8%, 7.3%, 15.2%, 22.3%, 30.2%, 19.4%, 21.4%, 7.0%, 5.3%, 17.8%, 62.3%, 50.4%, 42.1%, 15.4%, 70.2%, 58.7%, 43.3%, 10.2%, 55.7%, 48.9%, 40.3%, 19.1%, 58.2%, 55.9% or 38.9%.

6. A method of preparing a crude extract of Lindera aggregata, the method comprising: Step (1) grind and sieve the Loropetalum chinense, add water or alcohol organic solvent or ester organic solvent or ketone organic solvent as extractant, and stand; Step (2) reflux extraction, combine the extract, reduce pressure concentration, dry, and obtain the crude extract of Loropetalum chinense; ​ Preferably, in the step (1), the ratio of Loropetalum chinense to the extractant is 1:1-12 kg / L, and standing time is 0.5-18 h; in the step (2), reflux extraction is carried out for 1-4 times, and each extraction time is 0.2-4 h. Preferably, in the step (1), the sieve size is 18 mesh, the ratio of Loropetalum chinense to the extractant is 1:2-10 kg / L, standing time is 1-12 h, and in the step (2), reflux extraction is carried out for 1-3 times, and each extraction time is 0.5-2 h, and the extract is reduced pressure concentrated to relative density of 1.10-1.30 (60℃). Further preferably, in the step (1), the alcohol organic solvent is methanol, ethanol, propanol or butanol, the ester organic solvent is ethyl formate, ethyl acetate, propyl acetate, isopropyl acetate or butyl acetate, and the ketone organic solvent is acetone, butanone or pentanone. More preferably, the mass fraction of the alcohol organic solvent is 5%-100%, 10%-80%, 20%-60% or 40%-60%; more preferably, the mass fraction of the alcohol organic solvent is 20%, 40%, 60%, 80% or 100%.

7. A purified extract of Lindera benzoin, characterized in that: The purified extract of Loropetalum chinense is prepared by the following method: the crude extract of Loropetalum chinense according to any one of claims 1-5 is purified by macroporous resin elution to obtain different polar parts of the purified extract of Loropetalum chinense.

8. The purified extract of Loropetalum according to claim 7, wherein, The purified extract of Loropetalum chinense is prepared by the following method: the crude extract of Loropetalum chinense according to any one of claims 1-5 is diluted with distilled water, adsorbed by a macroporous resin column, eluted with deionized water, discarded, and then eluted with different concentrations of alcohol organic solvent, collected different concentrations of eluent, reduced pressure concentrated, and dried, to obtain the purified extract of Loropetalum chinense; the macroporous resin column is selected from one of the following types: HPD-300, HPD-400, HPD-450, HPD-600, HPD-826, ADS-17, ADS-21, AB-8, D101 or D301.

9. The extract of L. barbata of claim 8, wherein, The macroporous resin column is selected from one of the following types: AB-8, HPD-300, ADS-17 or D101; the adsorption flow rate is 1-8 mL·min -1 The macroporous resin has a diameter of 1-6 cm and a diameter-height ratio of 1:2-8, and the loading amount is 10-60 mL, and the static adsorption time is 2-24 h; the ion water elution amount is 1BV-6BV, the alcohol organic solvent is ethanol, the mass fraction is 10%-80%, and the amount is 2-10BV; Preferably, the adsorption flow rate is 2-6 mL·min -1 The macroporous resin has a diameter of 2-4 cm, a diameter-height ratio of 1:2-4, a loading amount of 20-40 mL, and a static adsorption time of 4-24 h. The ionized water elution amount is 2-6 BV. The mass fraction of the ethanol gradient elution is 10%, 30%, 50% or 70%, and the dosage is 2-8 BV; More preferably, the adsorption flow rate is 5 mL·min -1 More preferably, the adsorption flow rate is 5 mL·min More preferably, the adsorption flow rate is 5 mL·min 10. The purified extract of Loropetalum chinense according to any one of claims 7-9, wherein the total phenol content in the purified extract of Loropetalum chinense ranges from 1%-99%, 5%-95%, 6%-90%, 7%-85%, 8%-80%, 9%-78%, 10%-76%, 11%-74%, 12%-70%, 13%-68%, 14%-66%, 15%-64%, 16%-62%, 18%-60%, 20%-58%, 22%-56%, 24%-54%, 26%-52%, 28%-50%, 30%-48%, 32%-46%, 34%-44%, 32%-42%, 34%-40% or 36%-38%. Preferably, the total phenol content of the purified extract of the Magnoliae officinalis is 17.8%, 62.3%, 50.4%, 42.1%, 15.4%, 70.2%, 58.7%, 43.3%, 10.2%, 55.7%, 48.9%, 40.3%, 19.1%, 58.2% or 38.9%.

11. A method for preparing a purified extract of Loropetalum chinense, characterized in that: The purified extract of Magnoliae officinalis is prepared by the following method: the crude extract of Magnoliae officinalis according to any one of claims 1-5 is re-dissolved with distilled water, adsorbed by a macroporous resin column, eluted with deionized water, discarded, and then eluted with different concentrations of alcohol organic solvents, and the eluate of different concentrations is collected, concentrated under reduced pressure, and dried to obtain the purified extract of Magnoliae officinalis; the macroporous resin column is selected from one of the following types: HPD-300, HPD-400, HPD-450, HPD-600, HPD-826, ADS-17, ADS-21, AB-8, D101 or D301; The macroporous resin column is selected from one of the following types: AB-8, HPD-300, ADS-17 or D101; the adsorption flow rate is 1-8 mL·min -1 The macroporous resin has a diameter of 1-6 cm and a diameter-height ratio of 1:2-8, and the loading amount is 10-60 mL, and the static adsorption time is 2-24 h; the ion water elution amount is 1BV-6BV, the alcohol organic solvent is ethanol, the mass fraction is 10%-80%, and the amount is 2-10BV; Preferably, the adsorption flow rate is 2-6 mL·min -1 The macroporous resin has a diameter of 2-4 cm, a diameter-height ratio of 1:2-4, a loading amount of 20-40 mL, and a static adsorption time of 4-24 h. The ionized water has an elution amount of 2-6 BV. The gradient elution mass fraction of ethanol is 10%, 30%, 50% or 70%, and the amount is 2-8 BV; More preferably, the adsorption flow rate is 5 mL·min -1 More preferably, the adsorption flow rate is 5 mL·min More preferably, the adsorption flow rate is 5 mL·min 12. A traditional Chinese medicine preparation, characterized in that The traditional Chinese medicine preparation comprises the crude extract of Magnoliae officinalis according to any one of claims 1-5 or the purified extract of Magnoliae officinalis according to any one of claims 7-11 and a pharmaceutically acceptable excipient.

13. The traditional Chinese medicine preparation according to claim 12, characterized in that The traditional Chinese medicine preparation is selected from the following: tablets, capsules, buccal preparations, granules, pills, instant preparations, powders, pastes, pills, suspensions, solutions, injections, suppositories, pastes, sprays, drops, drop pills or patches.

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