Preparation method and application of peeled dendrobium officinale

Abstract

The invention relates to the technical field of traditional Chinese medicinal material processing, in particular to a preparation method and application of peeled dendrobium officinale. The preparation method comprises the following steps: adding dendrobium officinale into an enzyme-containing solution for enzymolysis, boiling for enzyme deactivation after enzymolysis is completed, taking out the treated dendrobium officinale, freezing and peeling; wherein enzyme in the enzyme-containing solution accounts for 0.02-1% of the weight of the dendrobium officinale, the enzymolysis temperature is 40-60 DEG C, and the enzymolysis time is 0.1-2 hours. The peeled dendrobium officinale prepared by the method can effectively promote dissolution of active ingredients such as polysaccharide and alkaloid, the activity of the active ingredients is kept, and the uniformity of crushed powder is improved.

Description

Technical Field

[0001] This invention relates to the field of traditional Chinese medicine processing technology, specifically to a method for preparing peeled Dendrobium officinale and its application. Background Technology

[0002] Dendrobium officinale, a traditional and precious Chinese medicinal herb, has its medicinal value recorded in medical classics throughout history. For example, the *Shennong Bencao Jing* (Shennong's Classic of Materia Medica) lists it as a superior herb, stating that it is "sweet and neutral in nature. It treats internal injuries, eliminates numbness, lowers qi, nourishes the five internal organs, strengthens yin, and with prolonged use, thickens the stomach and intestines." The *Bencao Gangmu* (Compendium of Materia Medica) records that it "nourishes yin and moistens dryness, benefits the stomach and generates fluids," and is particularly suitable for symptoms such as chronic cough due to lung deficiency and dry mouth and tongue. Modern research shows that Dendrobium officinale contains abundant active ingredients such as Dendrobium polysaccharides and amino acids, possessing pharmacological effects such as tonifying the kidneys and replenishing essence, strengthening muscles and bones, enhancing immunity, anti-fatigue, anti-oxidation, promoting digestion, lowering blood sugar and blood pressure, and anti-tumor activity. In application, Dendrobium officinale is usually processed into various products, such as Dendrobium officinale capsules, Dendrobium officinale powder, Dendrobium officinale tea, and Dendrobium officinale wine, which are widely used in the health food industry and are often used as dietary supplements in tea, wine, soup, and porridge.

[0003] However, the outer skin of fresh Dendrobium officinale contains a significant amount of cellulose, wax, and a small amount of impurities, exhibiting a distinct "iron skin" texture. This affects the extraction efficiency of active ingredients, product processing, taste, and applications. Currently, the main processing methods for Dendrobium officinale include drying, slicing, and pulverizing. However, traditional methods do not treat the outer skin, leading to: 1. Low dissolution rate of active ingredients: The dense outer skin structure hinders the release of active ingredients such as polysaccharides; 2. Impact on product quality: The waxy outer skin may impart a bitter taste, reducing the palatability of food; 3. Poor processing adaptability: In high-end pharmaceutical and cosmetic fields, unpeeled raw materials may affect the uniformity of formulations.

[0004] The outer skin is tightly adhered to the flesh, making it difficult to remove using conventional physical or chemical methods. Existing peeling techniques mostly employ mechanical friction or chemical soaking, but these methods have the following problems: 1. Mechanical methods easily damage the internal tissue, leading to the loss of active ingredients; 2. Chemical methods (such as strong alkali treatment) may leave harmful residues, failing to meet food safety standards. Therefore, there is an urgent need to develop an efficient, low-damage, and pollution-free method for preparing peeled Dendrobium officinale and to expand its application areas. Summary of the Invention

[0005] The present invention aims to at least solve one of the technical problems existing in the prior art. To this end, the present invention proposes a method for preparing peeled Dendrobium officinale.

[0006] This invention also proposes a peeled Dendrobium officinale.

[0007] This invention also proposes an application of peeled Dendrobium officinale.

[0008] According to one aspect of the present invention, a method for preparing peeled Dendrobium officinale is provided, comprising the following steps:

[0009] Dendrobium officinale was added to an enzyme-containing solution for enzymatic hydrolysis. After the enzymatic hydrolysis was completed, the enzyme was inactivated by boiling. The processed Dendrobium officinale was then removed, frozen, and peeled.

[0010] The enzyme in the enzyme-containing solution accounts for 0.02-1% of the weight of Dendrobium officinale, the enzymatic hydrolysis temperature is 40-60℃, and the enzymatic hydrolysis time is 0.5-2h.

[0011] In this invention, enzymatic hydrolysis is completed by softening the outer skin of Dendrobium officinale without damaging its internal structure.

[0012] In some embodiments of the present invention, the Dendrobium officinale is fresh Dendrobium officinale.

[0013] In some embodiments of the present invention, the Dendrobium officinale is cut into strips of about 5 cm.

[0014] In some embodiments of the present invention, the enzyme is a cellulase.

[0015] In some embodiments of the present invention, the enzyme activity is 50,000-150,000 U / g, for example, about 50,000 U / g, about 60,000 U / g, about 70,000 U / g, about 80,000 U / g, about 90,000 U / g, about 100,000 U / g, about 110,000 U / g, about 120,000 U / g, about 130,000 U / g, about 140,000 U / g, or about 150,000 U / g.

[0016] In some embodiments of the present invention, the pH value of the enzyme-containing solution is 6-7, for example, about 7.0.

[0017] In some embodiments of the present invention, the enzyme-containing solution is an aqueous solution of an enzyme, and the enzyme volume concentration of the enzyme-containing solution is 0.1-0.2%, for example, about 0.1%, about 0.15%, or about 0.2%.

[0018] In some embodiments of the present invention, the enzyme in the enzyme-containing solution accounts for 0.02-1% of the weight of Dendrobium officinale, for example, 0.1-1%, 0.2-1%, about 0.3%, about 0.4%, about 0.5%, about 0.6%, about 0.7%, about 0.8%, or about 0.9%.

[0019] In some embodiments of the present invention, the mass-to-volume ratio of the Dendrobium officinale to the enzyme-containing solution is 0.1-0.2 g: 1 mL, for example, about 0.12 g: 1 mL, about 0.15 g: 1 mL, about 0.16 g: 1 mL, or about 0.18 g: 1 mL.

[0020] In some embodiments of the present invention, the enzymatic hydrolysis temperature is 40-60°C, for example, about 50°C.

[0021] In some embodiments of the present invention, the enzymatic hydrolysis time is 0.5-2 hours, for example, about 1 hour or about 1.5 hours.

[0022] In some embodiments of the present invention, the temperature for boiling to inactivate the enzyme is 90-100°C, for example, about 95°C.

[0023] In some embodiments of the present invention, the boiling time for inactivating the enzyme is 5-15 minutes, for example, about 10 minutes.

[0024] In some embodiments of the present invention, the freezing process is carried out in a cryogenic plasma device for 30-60 seconds, for example, about 30 seconds, about 45 seconds, or about 60 seconds; the power of the cryogenic plasma device is 30-90W, for example, about 30W, about 50W, about 70W, or about 90W.

[0025] In some embodiments of the present invention, the peeling is a mechanical peeling method; for example, brushing with a brush or steel wool 1-3 times in both directions or using high-pressure water spray at a pressure of 0.2-0.5MPa to gently brush away the epidermis.

[0026] According to another aspect of the present invention, a peeled Dendrobium officinale is provided, which is prepared by the above-described method.

[0027] According to another aspect of the present invention, the application of the peeled Dendrobium officinale in the preparation of pharmaceuticals, health foods, and cosmetics is provided.

[0028] According to some embodiments of the present invention, at least the following beneficial effects are achieved:

[0029] The epidermal cells of *Dendrobium officinale* are arranged in a wavy pattern, with thickened outer and lateral walls and slight lignification; their main component is cellulose. This invention utilizes enzymatic treatment of *Dendrobium officinale* to enzymatically decompose the cellulose in the epidermis, simultaneously loosening the density of the epidermis. After enzyme inactivation, the *Dendrobium officinale* is frozen to rapidly cool it, achieving complete separation of the cellulose shell and internal tissues, thus efficiently obtaining fully peeled *Dendrobium officinale*. This method boasts high peeling efficiency and excellent peeling effect, significantly improving the taste, processing performance, and utilization efficiency of active ingredients, while minimizing damage to the internal structure of *Dendrobium officinale*.

[0030] Other features and advantages of the invention will be set forth in the description which follows, and will be apparent in part from the description, or may be learned by practicing the invention. Attached Figure Description

[0031] The present invention will be further described below with reference to the accompanying drawings and embodiments, wherein:

[0032] Figure 1 These are actual images of Dendrobium officinale after freezing (A) and after peeling (B) in Example 1 of the present invention;

[0033] Figure 2 These are actual images of Dendrobium officinale after freezing (A) and after peeling (B) in Example 2 of the present invention;

[0034] Figure 3 These are actual images of Dendrobium officinale after freezing (A) and after peeling (B) in Example 3 of the present invention;

[0035] Figure 4 These are actual images of Dendrobium officinale after freezing (A) and after peeling (B) in Example 4 of the present invention;

[0036] Figure 5 These are actual images of Dendrobium officinale after freezing (A) and after peeling (B) in Example 5 of the present invention;

[0037] Figure 6 These are actual images of Dendrobium officinale after freezing (A) and after peeling (B) in Example 6 of the present invention;

[0038] Figure 7 These are actual images of Dendrobium officinale after freezing (A) and after peeling (B) in Example 7 of the present invention;

[0039] Figure 8 These are actual images of Dendrobium officinale after freezing (A) and after peeling (B) in Comparative Example 1 of the present invention;

[0040] Figure 9 These are actual images of Dendrobium officinale after freezing (A) and after peeling (B) in Comparative Example 2 of the present invention;

[0041] Figure 10 These are actual images of Dendrobium officinale after freezing (A) and after peeling (B) in Comparative Example 3 of the present invention;

[0042] Figure 11 These are actual images of Dendrobium officinale after freezing (A) and after peeling (B) in Comparative Example 4 of the present invention;

[0043] Figure 12 These are actual images of Dendrobium officinale after freezing (A) and after peeling (B) in Comparative Example 5 of the present invention;

[0044] Figure 13The images show the internal structure of untreated Dendrobium officinale in Comparative Example 6 observed under a 10x microscope (A), the internal structure of Dendrobium officinale treated with hot water in Comparative Example 1 observed under a 10x microscope (B), the internal structure of enzyme-treated Dendrobium officinale in Example 1 observed under a 10x microscope (C), the epidermal structure of untreated Dendrobium officinale in Comparative Example 6 observed under a 20x microscope (D), the epidermal structure of Dendrobium officinale treated with hot water in Comparative Example 1 observed under a 20x microscope (E), and the epidermal structure of enzyme-treated Dendrobium officinale in Example 1 observed under a 20x microscope (F).

[0045] Figure 14 The standard curve for determining glucose content using the phenol-sulfuric acid method (A) and the detection results of polysaccharide content per gram obtained by the three methods (B) are shown.

[0046] Figure 15 The graph shows the powder uniformity results after grinding under normal conditions, compound enzyme treatment, and enzyme-assisted mechanical peeling.

[0047] Figure 16 The optimal peeling process flow chart for Dendrobium officinale. Detailed Implementation

[0048] The following will describe the concept and technical effects of the present invention clearly and completely with reference to the embodiments, so as to fully understand the purpose, features and effects of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are all within the scope of protection of the present invention.

[0049] When a numerical range is disclosed herein, the range is considered continuous and includes the minimum and maximum values ​​of the range, as well as every value between the minimum and maximum values. Furthermore, when the range refers to integers, it includes every integer between the minimum and maximum values ​​of the range. Additionally, when multiple ranges are provided to describe a feature or characteristic, the ranges may be combined. In other words, unless otherwise specified, all ranges disclosed herein should be understood to include any and all subranges to which they are incorporated.

[0050] Unless otherwise specified, in this invention, "about" means that the allowable error is within ±10%, further, within ±5%, and even further, within ±2%.

[0051] Unless otherwise specified in the examples, the procedures should be performed under standard conditions or conditions recommended by the manufacturer. Reagents or instruments whose manufacturers are not specified are all commercially available products.

[0052] In addition, the Dendrobium officinale used in the examples was collected from the core production area of ​​Wuyi County, Jinhua City, Zhejiang Province; all Dendrobium officinale were fresh, free from pests and diseases, uniform in size, and of consistent maturity from the same batch.

[0053] The cellulase used in the examples was purchased from Guangzhou Bixing Technology Co., Ltd., and the enzyme activity was 100,000 U / g.

[0054] Example 1

[0055] This embodiment provides a peeling process for Dendrobium officinale, including the following steps:

[0056] 1. Harvest approximately 8.5g of healthy Dendrobium officinale and cut it into 5cm sections;

[0057] 2. Add cellulase to water in a ratio of 100:0.15 (water to cellulase volume ratio), stir well to obtain an enzymatic hydrolysate (pH=7), and place it in an enzymatic hydrolysis tank for later use.

[0058] 3. Soak the Dendrobium officinale obtained in step 1 in 50 mL of the enzymatic hydrolysate obtained in step 2;

[0059] 4. Heat the enzymatic hydrolysate containing Dendrobium officinale from step 3 to 50°C and enzymatically hydrolyze for 60 minutes.

[0060] 5. Then heat the enzymatic hydrolysate containing Dendrobium officinale from step 4 to about 95°C and inactivate the enzyme for 10 minutes.

[0061] 6. Remove the Dendrobium officinale from the enzymatic hydrolysate in step 5, quickly wipe off the excess moisture on the surface, and then place it in a low-temperature plasma device (70W power, 45 seconds) for treatment.

[0062] 7. After spraying the Dendrobium officinale obtained in step 6 with high-pressure water (pressure 0.4 MPa), gently brush off the outer skin of the Dendrobium officinale. See the examples of Dendrobium officinale obtained in steps 6 and 7 respectively. Figure 1 Figures A and B are shown in the figure.

[0063] Example 2

[0064] This embodiment provides a peeling process for Dendrobium officinale, including the following steps:

[0065] 1. Harvest 8.5g of healthy Dendrobium officinale and cut it into 5cm pieces;

[0066] 2. Add cellulase to water in a ratio of 100:0.15 (water to cellulase volume ratio), stir well to obtain an enzymatic hydrolysate (pH=7), and place it in an enzymatic hydrolysis tank for later use.

[0067] 3. Soak the Dendrobium officinale obtained in step 1 in 50 mL of the enzymatic hydrolysate obtained in step 2;

[0068] 4. Heat the enzymatic hydrolysate containing Dendrobium officinale from step 3 to 40°C and enzymatically hydrolyze for 60 minutes.

[0069] 5. Heat the enzymatic hydrolysate containing Dendrobium officinale from step 4 to about 95°C and inactivate the enzyme for 10 minutes.

[0070] 6. Remove the Dendrobium officinale from the enzymatic hydrolysate in step 5, quickly wipe off the excess moisture on the surface, and then place it in a low-temperature plasma device (70W power, 45 seconds) for treatment.

[0071] 7. After spraying the Dendrobium officinale obtained in step S6 with high-pressure water (pressure 0.4MPa), gently brush off the outer skin of the Dendrobium officinale.

[0072] The Dendrobium officinale obtained in steps 6 and 7 are shown below. Figure 2 Figures A and B are shown in the figure.

[0073] Example 3

[0074] This embodiment provides a peeling process for Dendrobium officinale, including the following steps:

[0075] 1. Harvest 8.5g of healthy Dendrobium officinale and cut it into 5cm pieces;

[0076] 2. Add cellulase to water in a ratio of 100:0.15 (water to cellulase volume ratio), stir well to obtain an enzymatic hydrolysate (pH=7), and place it in an enzymatic hydrolysis tank for later use.

[0077] 3. Soak the Dendrobium officinale obtained in step 1 in 50 mL of the enzymatic hydrolysate obtained in step 2;

[0078] 4. Heat the enzymatic hydrolysate containing Dendrobium officinale from step 3 to 60°C and enzymatically hydrolyze for 60 minutes.

[0079] 5. Heat the enzymatic hydrolysate containing Dendrobium officinale from step 4 to about 95°C and inactivate the enzyme for 10 minutes.

[0080] 6. Remove the Dendrobium officinale from the enzymatic hydrolysate in step 5, quickly wipe off the excess moisture on the surface, and then place it in a low-temperature plasma device (70W power, 45 seconds) for treatment.

[0081] 7. After spraying the Dendrobium officinale obtained in step 6 with high-pressure water (pressure 0.4 MPa), gently brush off the outer skin of the Dendrobium officinale. See the examples of Dendrobium officinale obtained in steps 6 and 7 respectively. Figure 3 Figures A and B are shown in the figure.

[0082] Example 4

[0083] This embodiment provides a peeling process for Dendrobium officinale, including the following steps:

[0084] 1. Harvest 8.5g of healthy Dendrobium officinale and cut it into 5cm pieces;

[0085] 2. Add cellulase to water in a ratio of 100:0.15 (water to cellulase volume ratio), stir well to obtain an enzymatic hydrolysate (pH=7), and place it in an enzymatic hydrolysis tank for later use.

[0086] 3. Soak the Dendrobium officinale obtained in step 1 in 50 mL of the enzymatic hydrolysate obtained in step 2;

[0087] 4. Heat the enzymatic hydrolysate containing Dendrobium officinale from step 3 to 50°C and enzymatically hydrolyze for 30 minutes.

[0088] 5. Heat the enzymatic hydrolysate containing Dendrobium officinale from step 4 to about 95°C and inactivate the enzyme for 10 minutes.

[0089] 6. Remove the Dendrobium officinale from the enzymatic hydrolysate in step 5, quickly wipe off the excess moisture on the surface, and then place it in a low-temperature plasma device (70W power, 45 seconds) for treatment.

[0090] 7. After spraying the Dendrobium officinale obtained in step 6 with high-pressure water (pressure 0.4 MPa), gently brush off the outer skin of the Dendrobium officinale. See the examples of Dendrobium officinale obtained in steps 6 and 7 respectively. Figure 4 Figures A and B are shown in the figure.

[0091] Example 5

[0092] This embodiment provides a peeling process for Dendrobium officinale, including the following steps:

[0093] 1. Harvest 8.5g of healthy Dendrobium officinale and cut it into 5cm pieces;

[0094] 2. Add cellulase to water in a ratio of 100:0.15 (water to cellulase volume ratio), stir well to obtain an enzymatic hydrolysate (pH=7), and place it in an enzymatic hydrolysis tank for later use.

[0095] 3. Soak the Dendrobium officinale obtained in step 1 in 50 mL of the enzymatic hydrolysate obtained in step 2;

[0096] 4. Heat the enzymatic hydrolysate containing Dendrobium officinale from step 3 to 50°C and enzymatically hydrolyze for 120 minutes.

[0097] 5. Heat the enzymatic hydrolysate containing Dendrobium officinale from step 4 to about 95°C and inactivate the enzyme for 10 minutes.

[0098] 6. Remove the Dendrobium officinale from the enzymatic hydrolysate in step 5, quickly wipe off the excess moisture on the surface, and then place it in a low-temperature plasma device (70W power, 45 seconds) for treatment.

[0099] 7. After spraying the Dendrobium officinale obtained in step 6 with high-pressure water (pressure 0.4 MPa), gently brush off the outer skin of the Dendrobium officinale. See the examples of Dendrobium officinale obtained in steps 6 and 7 respectively. Figure 5 Figures A and B are shown in the figure.

[0100] Example 6

[0101] This embodiment provides a peeling process for Dendrobium officinale, including the following steps:

[0102] 1. Harvest 8.5g of healthy Dendrobium officinale and cut it into 5cm pieces;

[0103] 2. Add cellulase to water in a ratio of 100:0.10 (water to cellulase volume ratio), stir well to obtain an enzymatic hydrolysate (pH=7), and place it in an enzymatic hydrolysis tank for later use.

[0104] 3. Soak the Dendrobium officinale obtained in step 1 in 50 mL of the enzymatic hydrolysate obtained in step 2;

[0105] 4. Heat the enzymatic hydrolysate containing Dendrobium officinale from step 3 to 50°C and enzymatically hydrolyze for 60 minutes.

[0106] 5. Heat the enzymatic hydrolysate containing Dendrobium officinale from step 4 to about 95°C and inactivate the enzyme for 10 minutes.

[0107] 6. Remove the Dendrobium officinale from the enzymatic hydrolysate in step 5, quickly wipe off the excess moisture on the surface, and then place it in a low-temperature plasma device (70W power, 45 seconds) for treatment.

[0108] 7. After spraying the Dendrobium officinale obtained in step 6 with high-pressure water (pressure 0.4 MPa), gently brush off the outer skin of the Dendrobium officinale. See the examples of Dendrobium officinale obtained in steps 6 and 7 respectively. Figure 6 Figures A and B are shown in the figure.

[0109] Example 7

[0110] This embodiment provides a peeling process for Dendrobium officinale, including the following steps:

[0111] 1. Harvest 8.5g of healthy Dendrobium officinale and cut it into 5cm pieces;

[0112] 2. Add cellulase to water in a ratio of 100:0.20 (water to cellulase volume ratio), stir well to obtain an enzymatic hydrolysate (pH=7), and place it in an enzymatic hydrolysis tank for later use.

[0113] 3. Soak the Dendrobium officinale obtained in step 1 in 50 mL of the enzymatic hydrolysate obtained in step 2;

[0114] 4. Heat the enzymatic hydrolysate containing Dendrobium officinale from step 3 to 50°C and enzymatically hydrolyze for 60 minutes.

[0115] 5. Heat the enzymatic hydrolysate containing Dendrobium officinale from step 4 to about 95°C and inactivate the enzyme for 10 minutes.

[0116] 6. Remove the Dendrobium officinale from the enzymatic hydrolysate in step 5, quickly wipe off the excess moisture on the surface, and then place it in a low-temperature plasma device (70W power, 45 seconds) for treatment.

[0117] 7. After spraying the Dendrobium officinale obtained in step 6 with high-pressure water (pressure 0.4 MPa), gently brush off the outer skin of the Dendrobium officinale. See the examples of Dendrobium officinale obtained in steps 6 and 7 respectively. Figure 7 Figures A and B are shown in the figure.

[0118] Comparative Example 1

[0119] This comparative example provides a peeling process for Dendrobium officinale, which is carried out with reference to Example 1, except that step 2 is omitted and the enzymatic hydrolysate in step 3 is replaced with an equal amount of ultrapure water; specifically, it includes the following steps:

[0120] 1. Harvest 8.5g of healthy Dendrobium officinale and cut it into 5cm pieces;

[0121] 2. Soak the Dendrobium officinale obtained in step 1 in ultrapure water;

[0122] 3. Heat the ultrapure water containing Dendrobium officinale from step 2 to 50°C and treat for 60 minutes;

[0123] 4. Heat the ultrapure water containing Dendrobium officinale from step 3 to approximately 95°C and treat for 10 minutes.

[0124] 5. Remove the Dendrobium officinale from the ultrapure water in step 4, quickly wipe off the excess water on the surface, and then put it into the low-temperature plasma equipment (power 70W, time 45 seconds) for treatment.

[0125] 6. After spraying the Dendrobium officinale obtained in step 5 with high-pressure water (pressure 0.4 MPa), gently brush off the outer skin of the Dendrobium officinale. See the examples of Dendrobium officinale obtained in steps 5 and 6 respectively. Figure 8 Figures A and B are shown in the figure.

[0126] Comparative Example 2

[0127] This comparative example provides a peeling process for Dendrobium officinale, which is carried out with reference to Example 2, except that step 2 is omitted and the enzymatic hydrolysate in step 3 is replaced with an equal amount of ultrapure water; specifically, it includes the following steps:

[0128] 1. Harvest 8.5g of healthy Dendrobium officinale and cut it into 5cm pieces;

[0129] 2. Soak the Dendrobium officinale obtained in step 1 in ultrapure water;

[0130] 3. Heat the ultrapure water containing Dendrobium officinale from step 2 to 40°C and treat for 60 minutes;

[0131] 4. Heat the ultrapure water containing Dendrobium officinale from step 3 to approximately 95°C and treat for 10 minutes.

[0132] 5. Remove the Dendrobium officinale from the ultrapure water in step 4, quickly wipe off the excess water on the surface, and then put it into the low-temperature plasma equipment (power 70W, time 45 seconds) for treatment.

[0133] 6. After spraying the Dendrobium officinale obtained in step 5 with high-pressure water (pressure 0.4 MPa), gently brush off the outer skin of the Dendrobium officinale. See the examples of Dendrobium officinale obtained in steps 5 and 6 respectively. Figure 9 Figures A and B are shown in the figure.

[0134] Comparative Example 3

[0135] This comparative example provides a peeling process for Dendrobium officinale, which is carried out with reference to Example 3, except that step 2 is omitted and the enzymatic hydrolysate in step 3 is replaced with an equal amount of ultrapure water; specifically, it includes the following steps:

[0136] 1. Harvest 8.5g of healthy Dendrobium officinale and cut it into 5cm pieces;

[0137] 2. Soak the Dendrobium officinale obtained in step 1 in ultrapure water;

[0138] 3. Heat the ultrapure water containing Dendrobium officinale from step 2 to 60°C and treat for 60 minutes;

[0139] 4. Heat the ultrapure water containing Dendrobium officinale from step 3 to approximately 95°C and treat for 10 minutes.

[0140] 5. Remove the Dendrobium officinale from the ultrapure water in step 4, quickly wipe off the excess water on the surface, and then put it into the low-temperature plasma equipment (power 70W, time 45 seconds) for treatment.

[0141] 6. After spraying the Dendrobium officinale obtained in step 5 with high-pressure water (pressure 0.4 MPa), gently brush off the outer skin of the Dendrobium officinale. See the examples of Dendrobium officinale obtained in steps 5 and 6 respectively. Figure 10 Figures A and B are shown in the figure.

[0142] Comparative Example 4

[0143] This comparative example provides a peeling process for Dendrobium officinale, which is carried out with reference to Example 4, except that step 2 is omitted and the enzymatic hydrolysate in step 3 is replaced with an equal amount of ultrapure water; specifically, it includes the following steps:

[0144] 1. Harvest 8.5g of healthy Dendrobium officinale and cut it into 5cm pieces;

[0145] 2. Soak the Dendrobium officinale obtained in step 1 in ultrapure water;

[0146] 3. Heat the ultrapure water containing Dendrobium officinale from step 2 to 50°C and treat for 30 minutes;

[0147] 4. Heat the ultrapure water containing Dendrobium officinale from step 3 to approximately 95°C and treat for 10 minutes.

[0148] 5. Remove the Dendrobium officinale from the ultrapure water in step 4, quickly wipe off the excess water on the surface, and then put it into the low-temperature plasma equipment (power 70W, time 45 seconds) for treatment.

[0149] 6. After spraying the Dendrobium officinale obtained in step 5 with high-pressure water (pressure 0.4 MPa), gently brush off the outer skin of the Dendrobium officinale. See the examples of Dendrobium officinale obtained in steps 5 and 6 respectively. Figure 11 Figures A and B are shown in the figure.

[0150] Comparative Example 5

[0151] This comparative example provides a peeling process for Dendrobium officinale, which is carried out with reference to Example 5, except that step 2 is omitted and the enzymatic hydrolysate in step 3 is replaced with an equal amount of ultrapure water; specifically, it includes the following steps:

[0152] 1. Harvest 8.5g of healthy Dendrobium officinale and cut it into 5cm pieces;

[0153] 2. Soak the Dendrobium officinale obtained in step 1 in ultrapure water;

[0154] 3. Heat the ultrapure water containing Dendrobium officinale from step 2 to 50°C and treat for 120 minutes;

[0155] 4. Heat the ultrapure water containing Dendrobium officinale from step 3 to approximately 95°C and treat for 10 minutes.

[0156] 5. Remove the Dendrobium officinale from the ultrapure water in step 4, quickly wipe off the excess water on the surface, and then put it into the low-temperature plasma equipment (power 70W, time 45 seconds) for treatment.

[0157] 6. After spraying the Dendrobium officinale obtained in step 5 with high-pressure water (pressure 0.4 MPa), gently brush off the outer skin of the Dendrobium officinale. See the examples of Dendrobium officinale obtained in steps 5 and 6 respectively. Figure 12 Figures A and B are shown in the figure.

[0158] Comparative Example 6

[0159] This comparative example provides a peeling process for Dendrobium officinale, including the following steps: after normal fresh Dendrobium officinale is left untreated, it is sprayed with high-pressure water (pressure 0.4MPa) and then gently brushed.

[0160] The results showed that, compared with Figure 8-12 compared to, Figure 1-7 Dendrobium officinale with good peeling efficiency is smoother and has fewer rough fibers on its surface after peeling. Figure 1 , 6 7. It is easy to peel, with no cellulose residue and a smooth and flat surface. Figure 2-5 The peeling process is moderately easy, and there is obvious cellulose residue on the surface. Figure 8-10 The peeling process is moderately easy, and there is obvious cellulose residue on the surface. Figure 11 , 12 It's difficult to peel, and a lot of cellulose remains, making it almost like not treating it at all.

[0161] Test case

[0162] 1. Testing the peeling rate of Dendrobium officinale

[0163] The testing method is as follows: After hot water and enzyme treatment, the sample is quickly placed in a -20℃ freezer. After freezing, it is weighed using a 0.01% analytical balance, retaining four decimal places. After weighing, it is sprayed with high-pressure water (0.4MPa pressure) and then gently brushed before weighing again.

[0164] Peeling rate = (Initial weight - Weight after peeling) / Initial weight × 100%.

[0165] The test results are shown in Table 1 below.

[0166] Table 1

[0167]

[0168]

[0169] 2. Microscopic observation

[0170] The surface of Dendrobium officinale consists of the cuticle and the epidermis. In this test case, Dendrobium officinale was cross-sectioned to examine the morphology of Dendrobium officinale under a microscope after normal treatment, hot water treatment, and enzyme-assisted mechanical peeling treatment.

[0171] The specific operating steps are as follows: Quickly cut the weighed Comparative Examples 1 and 6, and Example 1 horizontally. Place them on a glass slide, then cover them with coverslips. Observe the changes in internal structure under a 10x microscope, and observe the epidermal peeling under a 20x microscope, and take photos to record the results.

[0172] Depend on Figure 13 As shown in Figures A, B, and C, normal Dendrobium officinale has a compact internal structure with tightly packed fiber bundles. However, the internal structure of Dendrobium officinale treated with hot water becomes loose, and the cellulose begins to break down. The enzymes in Dendrobium officinale treated with enzymes are no different from those in normal Dendrobium officinale. The outer layer of normal Dendrobium officinale consists of a yellow cuticle and epidermis. Figure 13 As can be seen from Figures D, E, and F, the Dendrobium officinale after enzyme treatment has completely separated from the cuticle and epidermis.

[0173] 3. Polysaccharide extraction

[0174] The main active ingredient in Dendrobium officinale is polysaccharide. This test case examines the content of polysaccharide extracted after normal treatment, compound enzyme treatment, and enzyme-assisted mechanical peeling.

[0175] The specific operating steps are as follows: Take 10g of fresh Dendrobium officinale stems for each group. The normal group is Comparative Example 6. The compound enzyme group uses a pectinase:cellulase mass ratio of 1:3 (pectinase activity 30,000 U / g, cellulase activity 100,000 U / g) to prepare a compound enzyme solution, and the volume ratio of compound enzyme solution to pure water is 0.4:1. The enzymatic hydrolysis temperature is 50℃, the enzymatic hydrolysis time is 95min, then freeze at -20℃ for 75min, and blanch for 20min. The enzyme-assisted mechanical peeling group is Example 1. Place the above three groups in a three-necked flask and add 20 times the volume of pure water. Reflux extraction in a heating mantle for 3 hours at 95℃. Add 4 times the volume of anhydrous ethanol to precipitate, and incubate overnight at 4℃. Filter using a vacuum filter, retain the residue, add 2 times the volume of pure water to reconstitute, and freeze dry in a freeze dryer for 72 hours until anhydrous (pre-frozen at -60℃, vacuum degree 10Pa). The polysaccharide content was determined using the phenol-sulfuric acid method.

[0176] Depend on Figure 14 It can be seen that the polysaccharide extraction amount in Example 1 is not significantly different from that in Control Group 6, but the polysaccharide extraction amount of Dendrobium officinale treated with compound enzyme is low. This indicates that the enzyme-assisted mechanical peeling method does not damage the quality of the main effective components of Dendrobium officinale while peeling.

[0177] 4. Powder uniformity

[0178] The powder was uneven before the skin of Dendrobium officinale was removed. The powder uniformity was examined after normal treatment, compound enzyme treatment, and enzyme-assisted mechanical peeling treatment.

[0179] The specific operating steps are as follows: Take 10g of fresh Dendrobium officinale stems for each group. The normal group is Comparative Example 6. The compound enzyme group uses a pectinase:cellulase mass ratio of 1:3 (pectinase activity 30,000 U / g, cellulase activity 100,000 U / g) to prepare a compound enzyme solution, and the volume ratio of compound enzyme solution to pure water is 0.4:1. The enzymatic hydrolysis temperature is 50℃, the enzymatic hydrolysis time is 95min, then it is frozen at -20℃ for 75min and blanched for 20min. The enzyme-assisted mechanical peeling group is Example 1. Rinse with pure water to remove residual enzymes or impurities. Vacuum freeze-dry (pre-freeze at -40℃, vacuum degree 10Pa, dry for 48 hours) until the moisture content is ≤8%.

[0180] Depend on Figure 15 It is evident that the powder particles in the untreated normal group are uneven, with a large amount of cellulose outer skin residue. Although the amount of cellulose outer skin was reduced after the compound enzyme treatment, the powder was still uneven and showed no significant difference from the normal group. After the enzyme-assisted mechanical peeling method, the powder was significantly different from the other two groups, with less cellulose outer skin, higher powder uniformity, and easier sieving.

[0181] In summary, this invention yields the optimal peeling process for Dendrobium officinale as follows: Figure 16 As shown.

[0182] The above description provides a detailed account of the embodiments of the present invention. However, the present invention is not limited to the above embodiments. Within the scope of knowledge possessed by those skilled in the art, various changes can be made without departing from the spirit of the present invention. Furthermore, unless otherwise specified, the embodiments of the present invention and the features thereof can be combined with each other.

Claims

1. A method for preparing peeled Dendrobium officinale, characterized in that, Includes the following steps: Dendrobium officinale was added to an enzyme-containing solution for enzymatic hydrolysis. After the enzymatic hydrolysis was completed, the enzyme was inactivated by boiling. The processed Dendrobium officinale was then removed, frozen, and peeled. The enzyme in the enzyme-containing solution accounts for 0.02-1% of the weight of Dendrobium officinale, the enzymatic hydrolysis temperature is 40-60℃, and the enzymatic hydrolysis time is 0.1-2h.

2. The method according to claim 1, characterized in that, The enzyme is a cellulase, and the enzyme activity is 50,000-150,000 U / g.

3. The method according to claim 1, characterized in that, The pH value of the enzyme-containing solution is 6-7.

4. The method according to claim 1, characterized in that, The enzyme-containing solution is an aqueous solution of the enzyme, and the enzyme volume concentration of the enzyme-containing solution is 0.1-0.2%.

5. The method according to claim 4, characterized in that, The mass ratio of the Dendrobium officinale to the volume of the enzyme-containing solution is 0.1-0.2 g: 1 mL.

6. The method according to claim 1, characterized in that, The temperature for boiling to inactivate the enzyme is 90-100℃, and the boiling time for inactivating the enzyme is 5-15 minutes.

7. The method according to claim 1, characterized in that, The freezing process is carried out in a low-temperature plasma device for 30-60 seconds, and the power of the low-temperature plasma device is 30-90W.

8. The method according to claim 1, characterized in that, The peeling is performed mechanically; preferably, the peeling is performed by brushing with a brush or steel wool 1-3 times in both directions or by gently brushing with high-pressure water spray at a pressure of 0.2-0.5MPa to remove the epidermis.

9. A peeled Dendrobium officinale, characterized in that, Prepared by the method according to any one of claims 1-8.

10. The application of the peeled Dendrobium officinale as described in claim 9 in the preparation of pharmaceuticals, health foods, and cosmetics.

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