Microbial biopesticide for preventing and treating wheat scab

Abstract

The invention belongs to the technical field of biopesticides, and particularly relates to a microbial biopesticide for preventing and treating wheat scab. The invention relates to a microbial biopesticide. The microbial biopesticide is prepared by binary compounding of ceriporia lacerata, physcion, L-alkannin or honokiol according to a certain mass ratio. According to the invention, the ceriporia lacerata GXMS1 is compounded with the existing bioactive bactericidal component physcion, L-alkannin or honokiol, and compared with the single use of the ceriporia lacerata GXMS1, the control effect on the wheat scab can be improved. The microbial biopesticide provided by the invention is environment-friendly, and compared with the use of chemical agents, the microbial biopesticide provided by the invention can reduce adverse effects such as drug resistance, environmental pollution and the like caused by chemical prevention and treatment, conforms to the ideas of green and sustainable development of agriculture, and can provide support for developing biopesticides for preventing and treating gibberellic disease.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of biological pesticides, and particularly relates to a microbial biological pesticide for preventing and treating wheat scab. BACKGROUND

[0002] Wheat scab, also known as wheat head blight, is a plant disease caused by Fusarium spp. Fusarium , mainly Fusarium graminearum. Fusarium graminearum In the early stage of infection by Fusarium graminearum, water-stained spots appear on the leaves of the infected plants, and then expand into yellow-brown or red-brown stripes. After the ear is infected, the grains shrink, and even the whole ear dies, resulting in a reduction in wheat yield. More seriously, Fusarium graminearum can produce mycotoxins such as deoxynivalenol, which have strong carcinogenic, teratogenic and immunosuppressive effects, and pose a great threat to human health.

[0003] Chemical control is a control method that uses chemical agents to inhibit or kill pathogenic fungi. Chemical control of wheat scab has the advantages of high efficiency and convenience, but long-term use of the same or similar chemical agents can lead to drug resistance of pathogenic fungi, and also has the disadvantages of environmental pollution, destruction of ecological balance and harm to human health. At present, the wheat scab fungus has developed different degrees of resistance to many chemical fungicides, resulting in a reduction in control effect.

[0004] Biological control is a method of using organisms and their metabolites in nature to control diseases. It is environmentally friendly, reduces the adverse effects of chemical control on the environment and human health, and meets the green and sustainable development concept. It is also the main research strategy for controlling crop diseases at present.

[0005] The present application belongs to the technical field of biological pesticides, and particularly relates to a microbial biological pesticide for preventing and treating wheat scab.

[0006] The present application belongs to the technical field of biological pesticides, and particularly relates to a microbial biological pesticide for preventing and treating wheat scab.

[0007] The information disclosed in this Background section is only for the purpose of increasing an understanding of the general context of the present application and should not be taken as an acknowledgement or any form of suggestion that this information forms prior art that is already known in any art. SUMMARY

[0008] The present application aims to provide a microbial biopesticide for preventing and treating wheat scab, which is compounded with the existing agents by using trametes lacryans GXMS1, so as to improve the prevention and treatment effect on wheat scab, is friendly to the environment, conforms to the concept of green and sustainable development, and can provide support for developing biological pesticides for preventing and treating wheat scab.

[0009] To achieve the above-mentioned purpose, the present application provides the following technical solutions. A microbial biopesticide, which is compounded by trametes lacryans and emodin methyl ether, left-handed shikonin or magnolol in a certain mass ratio.

[0010] More specifically, the trametes lacryans is 15 million cfu / g of trametes lacryans GXMS1 mother drug.

[0011] More specifically, the mass ratio of the trametes lacryans and the emodin methyl ether is 1:15-3.

[0012] More specifically, the mass ratio of the trametes lacryans and the left-handed shikonin is 1-20:10-1.

[0013] More specifically, the mass ratio of the trametes lacryans and the magnolol is 1-7:3-1.

[0014] The present application also provides the application of the microbial biopesticide in preventing and treating wheat scab.

[0015] More specifically, the pathogenic bacteria of the wheat scab is fusarium graminearum Fusarium graminearum .

[0016] The present application also provides a microbial biopesticide preparation, which comprises the microbial biopesticide.

[0017] More specifically, the mass of the microbial biopesticide accounts for 0.75-80% of the total mass of the microbial biopesticide preparation.

[0018] Compared with the prior art, the present application has the following beneficial effects: The present application can improve the prevention and treatment effect on wheat scab by compounding the tremellax GXMS1 with the existing bioactive fungicidal component emodin, shikonin or honokiol, compared with the use of tremellax GXMS1 alone. The microbial biopesticide of the present application is environmentally friendly, and compared with the use of chemical pesticides, it can reduce the adverse effects such as drug resistance and environmental pollution caused by chemical prevention and treatment, and conforms to the concept of green and sustainable development of agriculture, and can provide support for the development of biopesticides for preventing and treating scab. DETAILED DESCRIPTION

[0019] The following will be a clear and complete description of the technical solutions of the present application. Obviously, the described embodiments are part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0020] Example: prevention and treatment of pesticides 1. Test strain Fusarium graminearum (Schw.) S. Ito Fusarium graminearum , isolated from diseased wheat ear disease samples. Fusarium graminearum was inoculated into PDA medium for propagation culture, ready for use.

[0021] 2. Test medium PDA medium: peeled potato 200g, glucose 20g, agar 15g, add distilled water to 1000mL, natural pH value.

[0022] 3. Test agent 1500 million cfu / g of tremellax GXMS1 mother drug (Sichuan Jinzhu Ecological Agriculture Technology Co., Ltd.), 98% emodin (Shanghai Yuan Ye Biological Technology Co., Ltd.), 98% shikonin (CAS: 517-88-4, Shanghai Zeye Biological Technology Co., Ltd.), 99% honokiol (Hubei Baodu Chemical Co., Ltd.) Emodin, shikonin and honokiol were dissolved with solvent first, then diluted with 0.1% Tween-80 aqueous solution to prepare single agent mother liquor; the tremellax GXMS1 mother drug (100% by mass) was directly added to 0.1% Tween-80 aqueous solution to prepare single agent mother liquor. According to the results of the previous pre-test, multiple groups of matching ratios were set, and each single agent mother liquor and each group of matching agent were diluted with 0.1% Tween-80 aqueous solution to 6 mass concentration gradients. The above agents were prepared and used immediately.

[0023] 4. Test method The preliminary compatibility test found that the compatibility of Trametes ochracea GXMS1 with berberine, thymol or eugenol was poor, and the compatibility with physcion, shikonin or honokiol was good. Therefore, physcion, shikonin and honokiol were selected as the complexing factors of Trametes ochracea GXMS1.

[0024] The virulence of each single agent and each group of mixed agents on the test strain Fusarium graminearum was determined by mycelial growth rate method. Specifically, the test drug solution was mixed with PDA medium (cooled to about 50°C) in a conical flask at a volume ratio of 1:9, and then an equal amount was poured into a glass Petri dish with a diameter of 9 cm, 10 mL per dish. The drug-containing plates with corresponding concentrations were cooled, and each mass concentration of drug solution was set with 3 replicates, 3 plates per replicate, and 0.1% Tween-80 aqueous solution treatment as a blank control.

[0025] A 6mm-diameter fungus cake was cut from the edge of the test strain, and the fungus cake was inoculated in the center of the plate, with 1 fungus cake per plate. The plates were placed in a 25°C incubator and incubated until the colony diameter of the blank control reached 7cm. The colony diameter was measured by cross method, and the mycelial growth inhibition rate of different treatments was calculated.

[0026]

[0027] 5. Data analysis Linear regression was performed with the logarithmic value of fungicide concentration as x and the probability value of mycelial growth inhibition rate as y to obtain the virulence regression equation and the virulence EC 50 of the fungicide to the target pathogen. Sun Yunpei method was used to calculate the co-toxicity coefficient (CTC).

[0028]

[0029] In the above formula: ATI --the actual virulence index of the mixed agent; S --the EC 50 of the standard fungicide, with units of mg / L; M --the EC 50 of the mixed agent, with units of mg / L.

[0030]

[0031] In the above formula: TTI --the theoretical virulence index of the mixed agent; TI A --the virulence index of the A agent; P A --the percentage content of the A agent in the mixed agent, with units of percentage (%); TI B --the virulence index of the B agent; PB -- the percentage content of the B agent in the mixture, in percentage (%).

[0032]

[0033] In the above formula: CTC -- the coefficient of joint toxicity; ATI -- the measured toxicity index of the mixture; TTI -- the theoretical toxicity index of the mixture.

[0034] 6. Agent evaluation According to the coefficient of joint toxicity (CTC), the type of joint action of the agent is evaluated: CTC≤80 is antagonism, 80<CTC<120 is additive effect, and CTC≥120 is synergistic effect, and the results are shown in Tables 1-3.

[0035]

[0036] As shown in Table 1, when 15 million cfu / g of Inonotus hispidus GXMS1 is mixed with physcion at a mass ratio of 1:15-3, the coefficient of joint toxicity of the mixed combination on Fusarium graminearum is greater than 120, showing synergistic effect.

[0037]

[0038] As shown in Table 2, when 15 million cfu / g of Inonotus hispidus GXMS1 is mixed with L-shikimic acid at a mass ratio of 1-20:10-1, the coefficient of joint toxicity of the mixed combination on Fusarium graminearum is greater than 120, showing synergistic effect.

[0039]

[0040] As shown in Table 3, when 15 million cfu / g of Inonotus hispidus GXMS1 is mixed with honokiol at a mass ratio of 1-7:3-1, the coefficient of joint toxicity of the mixed combination on Fusarium graminearum is greater than 120, showing synergistic effect.

[0041] In summary, the present application mixes Inonotus hispidus GXMS1 with the existing bioactive fungicidal ingredients physcion, L-shikimic acid or honokiol, which can improve the control effect on Fusarium graminearum compared with the use of Inonotus hispidus GXMS1 alone. The microbial biopesticide of the present application is environmentally friendly, and compared with the use of chemical agents, it can reduce the adverse effects such as drug resistance and environmental pollution caused by chemical control, and conforms to the concept of green and sustainable development of agriculture, and can provide support for the development of biopesticides for the prevention and control of Fusarium graminearum.

[0042] The foregoing description of specific exemplary embodiments of the application has been presented for the purposes of illustration and description. It is not intended to be exhaustive or to limit the application to the precise forms disclosed, and obviously many modifications and variations are possible in light of the above teaching. It is intended that the scope of the application be limited not with this detailed description, but rather by the claims appended hereto.

Claims

1. A microbial biopesticide, characterized in that, The microbial biopesticide is a binary compound of *Phellinus laceratus* with emodin methyl ether, levorotatory shikonin, or magnolol in a certain mass ratio.

2. The microbial biopesticide according to claim 1, characterized in that, The *Phenothizoctonia solani* strain is a 15 million CFU / g *Phenothizoctonia solani* GXMS1 mother drug.

3. The microbial biopesticide according to claim 1, characterized in that, The mass ratio of the compound *Polyporus laceratus* to emodin methyl ether is 1:15-3.

4. The microbial biopesticide according to claim 1, characterized in that, The mass ratio of the compound *Porphyromonas lacera* to levorotatory shikonin is 1-20:10-1.

5. The microbial biopesticide according to claim 1, characterized in that, The mass ratio of the compound *Porphyromonas lacera* to magnolol is 1-7:3-1.

6. The application of the microbial pesticide according to any one of claims 1-5 in the prevention and control of wheat scab.

7. The application according to claim 6, characterized in that, The pathogen causing wheat scab is Fusarium graminearum. Fusarium graminearum .

8. A microbial pesticide formulation, characterized in that, The microbial pesticide formulation includes the microbial biological pesticide according to any one of claims 1-5.

9. The microbial pesticide formulation according to claim 8, characterized in that, The mass of the microbial biopesticide accounts for 0.75-80% of the total mass of the microbial pesticide formulation.

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