Composition with anti-aging, firming, improving and wrinkle-removing effects and preparation method thereof

Abstract

The invention discloses a composition with anti-aging, firming, improving and wrinkle-removing effects and a preparation method, and relates to the technical field of cosmetics. The composition provided by the invention comprises a humectant A, a humectant B, a humectant C, a humectant D, a skin conditioner A, a skin conditioner B, a skin conditioner C, a wrinkle-removing functional agent A, a wrinkle-removing functional agent B, a wrinkle-removing functional agent C, an emulsifier, a thickener, a preservative and water. The invention also provides a preparation method of the composition. Compared with the prior art, the composition with the anti-aging, firming, improving and wrinkle-removing effects, prepared by the invention, has relatively high bioavailability and wrinkle-removing effect.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, specifically to a composition and its preparation method that have anti-aging, firming, lifting and wrinkle-reducing effects. Background Technology

[0002] With the deepening of research into the biology of skin aging, active peptide ingredients have been widely used in high-end cosmetics due to their remarkable anti-aging effects. Among them, acetyl hexapeptide-8, as a neurotransmitter inhibitory peptide, can competitively inhibit the formation of the SNARE complex, blocking the release of catecholamines and acetylcholine, thereby effectively reducing muscle contraction and fading dynamic wrinkles and fine lines caused by repeated contractions of facial muscles. It is a safe ingredient that can achieve a Botox-like effect.

[0003] However, acetyl hexapeptide-8 faces two major technical bottlenecks in practical applications: First, as a hydrophilic macromolecular peptide, it is difficult to penetrate the stratum corneum barrier of human skin, resulting in low bioavailability and greatly limiting its full efficacy; second, its stability in formulation systems is easily affected by the environment. Factors such as pH, temperature, metal ions, and long-term storage may lead to its degradation and inactivation, thus affecting the long-term efficacy and shelf life of the product. Currently, most related products on the market directly add acetyl hexapeptide-8 solutions or powders, lacking an effective delivery system to ensure its transdermal absorption and stability. Although there have been some reports on liposome encapsulation technology, ordinary liposomes often suffer from structural instability, are prone to aggregation, precipitation, or leakage of active ingredients during storage, and similarly cannot guarantee the efficacy and quality of the final product.

[0004] CN120324304A discloses an anti-wrinkle composition containing acetyl hexapeptide-8, its preparation method, and its application. This anti-wrinkle composition includes polypeptide liposomes, *Chrysanthemum indicum* extract, *Stevia repens* leaf / stem extract, and *Coccasionia sambac* seed extract. The polypeptide liposomes comprise a lipid membrane and polypeptides located within the lipid membrane. The polypeptides include acetyl hexapeptide-8, copper peptide, and nonapeptide-1. Acetyl hexapeptide-8 effectively relaxes muscles by reducing acetylcholine release, resulting in a good immediate anti-wrinkle effect. The addition of copper peptide and nonapeptide-1 promotes collagen expression and stimulates dermal regeneration, leading to a good long-lasting anti-wrinkle effect. Simultaneously, the polypeptide combination is delivered via liposomes, enhancing its transdermal properties and long-lasting anti-wrinkle effect. However, these polypeptide liposomes are relatively unstable and prone to leakage of active ingredients during storage.

[0005] Therefore, developing an effective composition that can efficiently deliver acetyl hexapeptide-8, significantly improve its transdermal absorption rate and stability, and simultaneously integrate multiple anti-aging mechanisms has become a pressing technical problem to be solved in this field. Summary of the Invention

[0006] In view of the aforementioned deficiencies in the prior art, the present invention provides a composition with anti-aging, firming, lifting, and wrinkle-reducing effects. This composition, through innovative liposome encapsulation technology, effectively solves the problems of poor transdermal absorption and instability of the core active peptide acetyl hexapeptide-8, thereby significantly improving its bioavailability and final wrinkle-reducing effect.

[0007] To achieve the above objectives, the present invention provides a composition with anti-aging, firming, lifting, and wrinkle-reducing effects, comprising, based on a 100g raw material system: Moisturizer A 5-10g; Moisturizer B 3-8g; Moisturizer C 0.1-0.8g; Moisturizer D 0.1-0.3g; Skin Conditioner A 1-5g; Skin conditioning agent B 0.05-0.5g; Skin conditioning agent C 0.05-0.3g; Anti-wrinkle functional agent A 0.5-2g; Anti-wrinkle functional agent B 0.5-2g; Anti-wrinkle functional agent C 0.5-2g; Emulsifier 0.05-0.2g; Thickener 0.1-0.3g; Preservative 0.05-0.5g; Add water to bring the total weight to 100g.

[0008] Preferably, the moisturizer A is glycerin.

[0009] Preferably, the moisturizer B, by mass percentage, comprises 28-35% triethylhexanoate, 3-6% sorbitol, 0.1-0.8% 1,2-hexanediol, 0.1-0.8% p-hydroxyacetophenone, 0.1-0.2% pentaerythritol tetra(bis-tert-butylhydroxyhydrocinnamic acid) ester, 0.08-0.15% acrylate / C10-30 alkanol acrylate crosspolymer, 0.005-0.015% sodium hydroxide, 0.005-0.015% sodium hyaluronate, 0.0001-0.001% CI 61565, 0.0001-0.001% β-carotene, 0.0001-0.0015% corn (ZEA MAYS) oil, 0.0001-0.0015% tocopherol, and the balance being water.

[0010] Preferably, the humectant C is 1,2-hexanediol.

[0011] Preferably, the humectant D is 1,3-propanediol.

[0012] Preferably, the skin conditioning agent A, by mass percentage, is composed of 3-4% silk fibroin, 1-2% L-hydroxyproline, 1-2% conchiolin powder, and the remainder apple fiber.

[0013] Preferably, the skin conditioning agent B, by mass percentage, is composed of 45-55% saccharide isomers, 3-8% 1,2-pentanediol, and the balance being water. Preferably, the skin conditioning agent C is composed of an equal mass mixture of water and glyceryl glucoside.

[0014] Preferably, the wrinkle-reducing functional agent A is composed of 0.05%-0.15% dipeptide diaminobutyryl benzylamide diacetate, 0.02-0.05% ethylhexylglycerin, 2-5% 1,2-hexanediol and the balance water by mass percentage.

[0015] Preferably, the wrinkle-reducing functional agent B, by mass percentage, is composed of 0.001-0.003% arginine / lysine polypeptide, 2-5% 1,2-hexanediol, 0.02-0.05% ethylhexylglycerin, and the balance being water.

[0016] Preferably, the wrinkle-reducing functional agent C, by mass percentage, is composed of 0.05-0.15% acetyl hexapeptide-8 or functional liposome-encapsulated peptide, 0.03-0.05% ethylhexylglycerin, 2-5% 1,2-hexanediol and the balance being water.

[0017] Preferably, the method for preparing the functional liposome-encapsulated peptide includes the following steps: The membrane material and membrane functional agent are added to an organic solvent and mixed and dissolved. The organic solvent is then removed by rotary evaporation to form a uniform lipid film. Active peptides are dissolved in an hydration medium to obtain a peptide-containing aqueous phase, which is then added to the above-mentioned lipid film for hydration to form a crude liposome dispersion. The crude liposome dispersion is then homogenized under high pressure to obtain a liposome core dispersion. Stabilizers were added to the liposome core dispersion for dispersion stabilization treatment, and finally dried to obtain powdered functional liposome-encapsulated peptides.

[0018] More preferably, the preparation method of the functional liposome-encapsulated peptide specifically includes the following steps: S1. Place 3-5g of hydrogenated lecithin, 0.3-0.7g of phytosterol, and 0.1-0.4g of membrane function agent in a round-bottom flask, add 40-60mL of anhydrous ethanol, and place in a water bath at 45-55℃. Mix and dissolve at 150-250rpm. Use a rotary evaporator to evaporate for 20-40min at a water bath temperature of 45-55℃, a rotation speed of 100-150rpm, and a pressure of -0.09MPa to -0.1MPa to completely remove the organic solvent and form a uniform lipid film on the inner wall of the flask. S2. Dissolve 0.5-1.5g of acetyl hexapeptide-8 in 150-250mL of PBS buffer (0.005-0.015mol / L, pH 7.2-7.6) to obtain a peptide-containing aqueous phase. Add the peptide-containing aqueous phase (preheated to 50-60℃) to the above lipid membrane for hydration at 50-60℃, 100-200rpm, and 45-75min to form a crude liposome dispersion. Circulate the crude liposome dispersion through a high-pressure homogenizer at an operating pressure of 12000-18000psi 3-7 times to obtain the liposome core dispersion. S3. Add 0.1-0.5g of stabilizer to the liposome core dispersion at 40-50℃, and mix and stir at 300-500rpm for 45-75min to obtain a functional liposome-encapsulated peptide concentrate; freeze-dry to obtain powdered functional liposome-encapsulated peptides.

[0019] More preferably, the membrane functional agent is selected from at least one of cetearyl alcohol polyether-6 olive oil ester and dipalmitoyl hydroxyproline; More preferably, the stabilizer is selected from one or more combinations of Antarctic spirochetal extract, sclerotium gum, and schistosome extract.

[0020] Preferably, the emulsifier is an acrylic (ester) / C10-30 alkanol acrylate crosspolymer; the thickener is triethanolamine; and the preservative is p-hydroxyacetophenone.

[0021] This invention also provides a method for preparing the composition with the above-mentioned anti-aging, firming, lifting and wrinkle-reducing effects, comprising the following steps: Step 1: Add water at 75-80℃ and 300-500rpm, accounting for 60-80% of the total water volume. Then add moisturizer A, moisturizer D, and skin conditioning agent A in sequence and mix for 15-25 minutes. Then add moisturizer B and homogenize at 1500-2500rpm for 3-5 minutes to obtain the emulsion base. Step 2: Cool the emulsion matrix to 38-42℃ at 400-600 rpm, and then add wrinkle-reducing functional agent A, wrinkle-reducing functional agent B, wrinkle-reducing functional agent C, skin conditioning agent B and skin conditioning agent C in sequence to obtain a mixed system. In this step, each ingredient should be stirred for 5-10 minutes to ensure that it is evenly mixed before adding the next one. Step 3: Continue stirring until the temperature of the mixed system drops to 35-38℃, then add the emulsifier and continue stirring for 15-25 minutes; then add the thickener and mix until the system thickens instantly and becomes translucent; then add the humectant C and preservative, add the remaining water to 100%, adjust the speed to 200-400 rpm, and stir for 20-30 minutes to obtain the raw material composition; Step 4: Discharge the obtained raw material composition, let it stand and age for 24-48 hours, and then fill it to obtain the finished product composition with anti-aging, firming, lifting and wrinkle-reducing effects.

[0022] The beneficial effects of this invention are: 1. Compared with existing technologies, this invention encapsulates hydrophilic acetyl hexapeptide-8 within a lipid bilayer to prepare a functional liposome-encapsulated peptide. This functional liposome-encapsulated peptide is then applied to the preparation of compositions with anti-aging, firming, lifting, and wrinkle-reducing effects. Liposomes exhibit similar compatibility with skin stratum corneum lipids, enabling them to fuse and promote the penetration of active ingredients through the skin barrier. In vitro skin permeability model tests show a significant increase in the transdermal permeability of acetyl hexapeptide-8, thereby greatly improving its bioavailability and making the wrinkle-reducing effect more direct and significant. Furthermore, the composition of this invention also contains multiple wrinkle-reducing functional agents such as dipeptide diaminobutyryl benzylamide diacetate and arginine / lysine peptides. These components synergistically work with liposome membrane functional agents to achieve a full-pathway anti-aging effect, from inhibiting expression lines and relaxing muscles to stimulating collagen and filling wrinkles, resulting in effects far superior to single-component formulations.

[0023] 2. Compared to existing technologies, this invention introduces membrane functional agents mixed with hydrogenated lecithin and phytosterols to first form a robust liposome core during the preparation of functional liposome-encapsulated peptides, which is then reinforced by external stabilizers. The selection of sclerotium gum and schistosome extract as stabilizers creates a strong steric hindrance and physical suspension network on the outside of the liposomes, effectively preventing aggregation, sedimentation, and leakage of active ingredients during long-term storage. Accelerated stability tests show that after being stored at 40°C for 3 months, the activity retention rate of acetyl hexapeptide-8 in the composition of this invention is significantly improved compared to the unencapsulated system. Detailed Implementation

[0024] The parameters for using specific chemical substances, and their sources.

[0025] Sugar isomer, PENTAVITIN®, catalog number 5038443, is sourced from DSM-Firmware.

[0026] Apple fiber preparation: 1000 kg of commercially available apple pomace (after washing and passing through a 60-mesh sieve) was mixed with 3000 kg of a 0.8 wt% sodium hydroxide aqueous solution and stirred continuously at 65°C for 80 min; then solid-liquid separation was performed, and the solid fibers were collected and then added to 2500 kg of a 4 wt% hydrogen peroxide aqueous solution and bleached at 55°C for 50 min; after bleaching, the fibers were washed with water until neutral, then transferred to a vacuum dryer and dried at 65°C for 5 h, and finally pulverized and passed through a 400-mesh sieve to obtain the finished apple fiber; Preparation of sericin: 1000 kg of crushed and washed waste silkworm cocoons (commercially available) are mixed with 25000 kg of water and 8 kg of sodium carbonate. The mixture is stirred at 98℃ and 500 rpm for 90 min to obtain a mixed solution. The mixed solution is then immediately subjected to hot filtration, and the filtrate is collected. The filtrate is concentrated to 1 / 5 of its original volume at 55℃ and -0.085 MPa to obtain a concentrated solution. Finally, the concentrated solution is sent to a spray drying tower, where the inlet air temperature is set to 170℃ and the outlet air temperature is set to 85℃ for drying. The powder is collected, which is the finished sericin product. Preparation of shell fibroin powder: 1000 kg of commercially available 80-mesh shell powder was mixed with 12000 kg of a 0.5 mol / L, pH 8.0 disodium EDTA solution and stirred at 4℃ and 100 rpm for 72 h to obtain a mixed solution. The mixed solution was centrifuged at 5000 rpm for 10 min, and the precipitate was collected. The precipitate was washed successively with 0.1 mol / L sodium hydroxide aqueous solution and water until the pH value was 7.0, and then washed with acetone and ethanol successively to obtain a protein matrix. Finally, the protein matrix was freeze-dried to obtain a powder, which was then ground through a 400-mesh sieve to obtain shell fibroin powder. Corn (ZEA MAYS) oil, cosmetic grade, commercially available; Acrylic (ester) crosspolymer / C10-30 alkanol acrylate crosspolymer, cosmetic grade, CAS No.: 176429-87-1; Triglyceride (ethylhexanoate), cosmetic grade, CAS No.: 7360-38-5; Glyceryl glucoside, cosmetic grade, CAS No.: 22160-26-5; L-Hydroxyproline, cosmetic grade, CAS No.: 51-35-4; Pentaerythritol tetra(bis-tert-butylhydroxyhydrocinnamic acid) ester, cosmetic grade, CAS No.: 6683-19-8; Ethylhexylglycerin, cosmetic grade, CAS No.: 70445-33-9; Dipeptide diaminobutyroyl benzylamide diacetate, cosmetic grade, CAS No.: 823202-99-9; Acetyl hexapeptide-8, cosmetic grade, CAS No.: 616204-22-9; β-carotene, cosmetic grade, CAS No.: 7235-40-7; CI 61565, cosmetic grade, CAS No.: 128-80-3; Arginine / Lysine peptide, cosmetic grade, CAS No.: 936616-33-0; Hydrogenated lecithin, cosmetic grade, CAS No.: 92128-87-5; Phytosterols, cosmetic grade, CAS No.: 83-46-5; Dipalmitoyl hydroxyproline, cosmetic grade, CAS No.: 41672-81-5; Cetearyl alcohol polyether-6 oleate, cosmetic grade, CAS No.: 226708-41-4; Antarctic spirochete extract, code: ALG104, sourced from ELICITYL, France; Sclerotium sclerotium gum, cosmetic grade, CAS No.: 39464-87-4; Schizotypalin, cosmetic grade, CAS No.: 9050-67-3; Example 1

[0027] A method for preparing a composition with anti-aging, firming, lifting, and wrinkle-reducing effects includes the following steps: Step 1: Add 60g of water at 78℃ and 400rpm, then add 8g of glycerin, 0.15g of 1,3-propanediol and 2.5g of skin conditioning agent A in sequence and mix for 20min; then add 5g of moisturizer B and homogenize at 2000rpm for 5min to obtain the emulsion base; Step 2: Cool the emulsion matrix to 40°C at 500 rpm, then add 1g of anti-wrinkle functional agent A, 1g of anti-wrinkle functional agent B, 1g of anti-wrinkle functional agent C, 0.3g of skin conditioning agent B and 0.1g of skin conditioning agent C in sequence to obtain a mixed system. In this step, each ingredient should be stirred for 10 minutes to ensure that it is evenly mixed before adding the next one. Step 3: Continue stirring until the temperature of the mixed system drops to 37℃. Add 0.15g of acrylate / C10-30 alkanol acrylate crosspolymer and continue stirring for 20min. Then add 0.15g of triethanolamine and mix until the system thickens instantly and becomes translucent. Then add 0.5g of 1,2-hexanediol and 0.4g of p-hydroxyacetophenone, and add the remaining 19.75g of water. Adjust the speed to 300rpm and stir for 30min to obtain the raw material composition. Step 4: Discharge the obtained raw material composition, let it stand for 48 hours to age, and then fill it to obtain the finished product composition with anti-aging, firming, lifting and wrinkle-reducing effects.

[0028] The moisturizer B, by weight percentage, is composed of 30% triethylhexanoate, 5% sorbitol, 0.5% 1,2-hexanediol, 0.5% p-hydroxyacetophenone, 0.15% pentaerythritol tetra(bis-tert-butylhydroxyhydrocinnamic acid), 0.1% acrylate / C10-30 alkanol acrylate crosspolymer, 0.01% sodium hydroxide, 0.01% sodium hyaluronate, 0.001% CI 61565, 0.001% β-carotene, 0.001% corn (ZEA MAYS) oil, 0.0001% tocopherol, and the balance being water.

[0029] The skin conditioning agent A, by weight percentage, is composed of 3.8% silk fibroin, 1.5% L-hydroxyproline, 1.2% conchiolin powder, and the balance apple fiber.

[0030] The skin conditioning agent B, by mass percentage, is composed of 50% saccharide isomers, 5% 1,2-pentanediol, and the balance water.

[0031] The skin conditioning agent C is composed of an equal mass mixture of water and glyceryl glucoside.

[0032] The wrinkle-reducing functional agent A, by mass percentage, is composed of 0.1% dipeptide diaminobutyryl benzylamide diacetate, 0.04% ethylhexylglycerin, 3% 1,2-hexanediol, and the balance being water.

[0033] The wrinkle-reducing functional agent B, by mass percentage, is composed of 0.002% arginine / lysine polypeptide, 3% 1,2-hexanediol, 0.04% ethylhexylglycerin, and the balance being water.

[0034] The wrinkle-reducing functional agent C, by mass percentage, is composed of 0.1% acetyl hexapeptide-8, 0.04% ethylhexylglycerin, 3% 1,2-hexanediol, and the balance being water. Example 2

[0035] A method for preparing a composition with anti-aging, firming, lifting, and wrinkle-reducing effects includes the following steps: Step 1: Add 60g of water at 78℃ and 400rpm, then add 8g of glycerin, 0.15g of 1,3-propanediol and 2.5g of skin conditioning agent A in sequence and mix for 20min; then add 5g of moisturizer B and homogenize at 2000rpm for 5min to obtain the emulsion base; Step 2: Cool the emulsion matrix to 40°C at 500 rpm, then add 1g of anti-wrinkle functional agent A, 1g of anti-wrinkle functional agent B, 1g of anti-wrinkle functional agent C, 0.3g of skin conditioning agent B and 0.1g of skin conditioning agent C in sequence to obtain a mixed system. In this step, each ingredient should be stirred for 10 minutes to ensure that it is evenly mixed before adding the next one. Step 3: Continue stirring until the temperature of the mixed system drops to 37℃. Add 0.15g of acrylate / C10-30 alkanol acrylate crosspolymer and continue stirring for 20min. Then add 0.15g of triethanolamine and mix until the system thickens instantly and becomes translucent. Then add 0.5g of 1,2-hexanediol and 0.4g of p-hydroxyacetophenone, and add the remaining 19.75g of water. Adjust the speed to 300rpm and stir for 30min to obtain the raw material composition. Step 4: Discharge the obtained raw material composition, let it stand for 48 hours to age, and then fill it to obtain the finished product composition with anti-aging, firming, lifting and wrinkle-reducing effects.

[0036] The moisturizer B, by weight percentage, is composed of 30% triethylhexanoate, 5% sorbitol, 0.5% 1,2-hexanediol, 0.5% p-hydroxyacetophenone, 0.15% pentaerythritol tetra(bis-tert-butylhydroxyhydrocinnamic acid), 0.1% acrylate / C10-30 alkanol acrylate crosspolymer, 0.01% sodium hydroxide, 0.01% sodium hyaluronate, 0.001% CI 61565, 0.001% β-carotene, 0.001% corn (ZEA MAYS) oil, 0.0001% tocopherol, and the balance being water.

[0037] The skin conditioning agent A, by weight percentage, is composed of 3.8% silk fibroin, 1.5% L-hydroxyproline, 1.2% conchiolin powder, and the balance apple fiber.

[0038] The wrinkle-reducing functional agent A, by mass percentage, is composed of 0.1% dipeptide diaminobutyryl benzylamide diacetate, 0.04% ethylhexylglycerin, 3% 1,2-hexanediol, and the balance being water.

[0039] The wrinkle-reducing functional agent B, by mass percentage, is composed of 0.002% arginine / lysine polypeptide, 3% 1,2-hexanediol, 0.04% ethylhexylglycerin, and the balance being water.

[0040] The wrinkle-reducing functional agent C, by mass percentage, is composed of 0.1% functional liposome-encapsulated peptides, 0.04% ethylhexylglycerin, 3% 1,2-hexanediol, and the balance being water.

[0041] The preparation method of the functional liposome-encapsulated peptide includes the following steps: S1. Place 4.25g hydrogenated lecithin, 0.5g phytosterol, and 0.25g dipalmitoyl hydroxyproline in a 250mL round-bottom flask, add 50mL anhydrous ethanol, and place in a 45℃ water bath. Mix and dissolve at 200rpm. Use a rotary evaporator to evaporate at a water bath temperature of 50℃, a rotation speed of 120rpm, and -0.095MPa until the organic solvent is completely removed, forming a uniform lipid film on the inner wall of the flask. S2. Add 1g of acetyl hexapeptide-8 to 200mL of 0.01mol / L, pH 7.4 PBS buffer and mix to dissolve, obtaining a peptide-containing aqueous phase; hydrate the above lipid membrane with 200mL of the peptide-containing aqueous phase preheated to 55℃, wherein the hydration temperature is 55℃, the rotation speed is 150rpm, and the hydration time is 60min, to form a crude liposome dispersion; homogenize the crude liposome dispersion using a high-pressure homogenizer at an operating pressure of 15000psi for 5 cycles to obtain the liposome core dispersion; S3. Add 0.3g of Antarctic spirulina extract to a liposome core dispersion at 45℃, mix and stir at 45℃ and 400rpm for 60min to obtain a functional liposome-encapsulated peptide concentrate; freeze dry to obtain functional liposome-encapsulated peptides. Example 3

[0042] A method for preparing a composition with anti-aging, firming, lifting, and wrinkle-reducing effects differs from Example 2 only in the preparation method of the functional liposome-encapsulated peptides. Specifically, step S3 is as follows: 0.3g of sclerotium gum was added to the liposome core dispersion at 45℃ and mixed and stirred at 45℃ and 400rpm for 60min to obtain a concentrated solution of functional liposome-encapsulated peptides; the solution was then freeze-dried to obtain functional liposome-encapsulated peptides. Example 4

[0043] A method for preparing a composition with anti-aging, firming, lifting, and wrinkle-reducing effects differs from Example 2 only in the preparation method of the functional liposome-encapsulated peptides. Specifically, step S3 is as follows: 0.3 g of schistosome was added to the liposome core dispersion at 45 °C and mixed and stirred at 45 °C and 400 rpm for 60 min to obtain a concentrated solution of functional liposome-encapsulated peptides; the solution was then freeze-dried to obtain functional liposome-encapsulated peptides. Example 5

[0044] A method for preparing a composition with anti-aging, firming, lifting, and wrinkle-reducing effects differs from Example 2 only in the preparation method of the functional liposome-encapsulated peptides. Specifically, step S3 is as follows: 0.15g of Antarctic spirulina extract and 0.15g of Sclerotium truncatum gum were added to a liposome core dispersion at 45℃ and mixed and stirred at 45℃ and 400rpm for 60min to obtain a functional liposome-encapsulated peptide concentrate; the concentrate was then freeze-dried to obtain the functional liposome-encapsulated peptide. Example 6

[0045] A method for preparing a composition with anti-aging, firming, lifting, and wrinkle-reducing effects differs from Example 2 only in the preparation method of the functional liposome-encapsulated peptides. Specifically, step S3 is as follows: 0.15g of Antarctic spirulina extract and 0.15g of schistosome were added to a liposome core dispersion at 45℃ and mixed and stirred at 45℃ and 400rpm for 60min to obtain a functional liposome-encapsulated peptide concentrate; the concentrate was then freeze-dried to obtain the functional liposome-encapsulated peptide. Example 7

[0046] A method for preparing a composition with anti-aging, firming, lifting, and wrinkle-reducing effects differs from Example 2 only in the preparation method of the functional liposome-encapsulated peptides. Specifically, step S3 is as follows: 0.15g of sclerotinia gum and 0.15g of schistosome were added to the liposome core dispersion at 45℃ and mixed and stirred at 45℃ and 400rpm for 60min to obtain a concentrated solution of functional liposome-encapsulated peptides; the solution was then freeze-dried to obtain functional liposome-encapsulated peptides. Example 8

[0047] A method for preparing a composition with anti-aging, firming, lifting, and wrinkle-reducing effects includes the following steps: Step 1: Add 60g of water at 78℃ and 400rpm, then add 8g of glycerin, 0.15g of 1,3-propanediol and 2.5g of skin conditioning agent A in sequence and mix for 20min; then add 5g of moisturizer B and homogenize at 2000rpm for 5min to obtain the emulsion base; Step 2: Cool the emulsion matrix to 40°C at 500 rpm, then add 1g of anti-wrinkle functional agent A, 1g of anti-wrinkle functional agent B, 1g of anti-wrinkle functional agent C, 0.3g of skin conditioning agent B and 0.1g of skin conditioning agent C in sequence to obtain a mixed system. In this step, each ingredient should be stirred for 10 minutes to ensure that it is evenly mixed before adding the next one. Step 3: Continue stirring until the temperature of the mixed system drops to 37℃. Add 0.15g of acrylate / C10-30 alkanol acrylate crosspolymer and continue stirring for 20min. Then add 0.15g of triethanolamine and mix until the system thickens instantly and becomes translucent. Then add 0.5g of 1,2-hexanediol and 0.4g of p-hydroxyacetophenone, and add the remaining 19.75g of water. Adjust the speed to 300rpm and stir for 30min to obtain the raw material composition. Step 4: Discharge the obtained raw material composition, let it stand for 48 hours to age, and then fill it to obtain the finished product composition with anti-aging, firming, lifting and wrinkle-reducing effects.

[0048] The moisturizer B, by weight percentage, is composed of 30% triethylhexanoate, 5% sorbitol, 0.5% 1,2-hexanediol, 0.5% p-hydroxyacetophenone, 0.15% pentaerythritol tetra(bis-tert-butylhydroxyhydrocinnamic acid), 0.1% acrylate / C10-30 alkanol acrylate crosspolymer, 0.01% sodium hydroxide, 0.01% sodium hyaluronate, 0.001% CI 61565, 0.001% β-carotene, 0.001% corn (ZEA MAYS) oil, 0.0001% tocopherol, and the balance being water.

[0049] The skin conditioning agent A, by weight percentage, is composed of 3.8% silk fibroin, 1.5% L-hydroxyproline, 1.2% conchiolin powder, and the balance apple fiber.

[0050] The wrinkle-reducing functional agent A, by mass percentage, is composed of 0.1% dipeptide diaminobutyryl benzylamide diacetate, 0.04% ethylhexylglycerin, 3% 1,2-hexanediol, and the balance being water.

[0051] The wrinkle-reducing functional agent B, by mass percentage, is composed of 0.002% arginine / lysine polypeptide, 3% 1,2-hexanediol, 0.04% ethylhexylglycerin, and the balance being water.

[0052] The preparation method of the functional liposome-encapsulated peptide includes the following steps: S1. Place 4.25g hydrogenated lecithin, 0.5g phytosterol, and 0.25g cetearyl alcohol polyether-6 olive oil ester in a 250mL round-bottom flask, add 50mL anhydrous ethanol, and place in a 45℃ water bath. Mix and dissolve at 200rpm. Use a rotary evaporator to evaporate at a water bath temperature of 50℃, a rotation speed of 120rpm, and -0.095MPa until the organic solvent is completely removed, forming a uniform lipid film on the inner wall of the flask. S2. Add 1g of acetyl hexapeptide-8 to 200mL of 0.01mol / L, pH 7.4 PBS buffer and mix to dissolve, obtaining a peptide-containing aqueous phase; hydrate the above lipid membrane with 200mL of the peptide-containing aqueous phase preheated to 55℃, wherein the hydration temperature is 55℃, the rotation speed is 150rpm, and the hydration time is 60min, to form a crude liposome dispersion; homogenize the crude liposome dispersion using a high-pressure homogenizer at an operating pressure of 15000psi for 5 cycles to obtain the liposome core dispersion; S3. Add 0.15g of Antarctic spirulina extract and 0.15g of Sclerotium sclerotiorum gum to a liposome core dispersion at 45℃, mix and stir at 45℃ and 400rpm for 60min to obtain a functional liposome-encapsulated peptide concentrate; freeze dry to obtain functional liposome-encapsulated peptides. Example 9

[0053] A method for preparing a composition with anti-aging, firming, lifting, and wrinkle-reducing effects differs from Example 8 only in the preparation method of the functional liposome-encapsulated peptides. Specifically, step S3 is as follows: 0.15g of Antarctic spirulina extract and 0.15g of schistosome were added to a liposome core dispersion at 45℃ and mixed and stirred at 45℃ and 400rpm for 60min to obtain a functional liposome-encapsulated peptide concentrate; the concentrate was then freeze-dried to obtain the functional liposome-encapsulated peptide. Example 10

[0054] A method for preparing a composition with anti-aging, firming, lifting, and wrinkle-reducing effects differs from Example 8 only in the preparation method of the functional liposome-encapsulated peptides. Specifically, step S3 is as follows: 0.15g of sclerotinia gum and 0.15g of schistosome were added to the liposome core dispersion at 45℃ and mixed and stirred at 45℃ and 400rpm for 60min to obtain a concentrated solution of functional liposome-encapsulated peptides; the solution was then freeze-dried to obtain functional liposome-encapsulated peptides.

[0055] Comparative Example 1 A method for preparing a composition with anti-aging, firming, lifting, and wrinkle-reducing effects differs from Example 2 only in the preparation method of the functional liposome-encapsulated peptides. Specifically, step S3 is as follows: S3. Mix and stir the liposome core dispersion at 45℃ and 400rpm for 60min to obtain a concentrated solution of functional liposome-encapsulated peptides; then freeze-dry to obtain functional liposome-encapsulated peptides.

[0056] Comparative Example 2 A method for preparing a composition with anti-aging, firming, lifting, and wrinkle-reducing effects differs from Example 8 only in the preparation method of the functional liposome-encapsulated peptides. Specifically, step S3 is as follows: S3. Mix and stir the liposome core dispersion at 45℃ and 400rpm for 60min to obtain a concentrated solution of functional liposome-encapsulated peptides; then freeze-dry to obtain functional liposome-encapsulated peptides.

[0057] Comparative Example 3 A method for preparing a composition with anti-aging, firming, lifting, and wrinkle-reducing effects differs from Example 8 only in the preparation method of the functional liposome-encapsulated peptides. Specifically, step S1 is as follows: 4.5g of hydrogenated lecithin and 0.5g of phytosterol were placed in a 250mL round-bottom flask, and 50mL of anhydrous ethanol was added. The flask was placed in a 45℃ water bath and mixed and dissolved at 200rpm. Using a rotary evaporator, the mixture was evaporated at a water bath temperature of 50℃, a rotation speed of 120rpm, and a pressure of -0.095MPa until the organic solvent was completely removed, forming a uniform lipid film on the inner wall of the flask.

[0058] Test Example 1 Performance testing of peptides encapsulated in functional liposomes Encapsulation efficiency determination The functional liposome-encapsulated peptides prepared in Examples 2-10 and Comparative Examples 1-3 of this invention were used as test samples. Free peptides were separated by ultrafiltration centrifugation (molecular weight cutoff 10 kDa). The content of acetyl hexapeptide-8 encapsulated in the liposomes was determined by high-performance liquid chromatography (HPLC), and the encapsulation efficiency was calculated. The specific test methods are as follows: Total peptide content determination: Accurately measure 1.0 mL of functional liposome-encapsulated peptide concentrate, add 9.0 mL of methanol for demulsification, vortex for 5 min, and then centrifuge at 12000 rpm for 15 min; take the supernatant and determine the concentration of acetyl hexapeptide-8 by HPLC, denoted as C. 总 ; Determination of free peptide content: Accurately measure 1.0 mL of the same liposome dispersion, add it to an ultrafiltration centrifuge tube, and centrifuge at 4000 rpm for 30 min; collect the filtrate, and determine the concentration of acetyl hexapeptide-8 by HPLC, denoted as C0. 游离 ; The formula for calculating encapsulation efficiency (EE%) is as follows: Encapsulation efficiency (EE%) = (C 总 -C游离 ) / C 总 ×100%.

[0059] Each sample was measured in triplicate, and the average value was taken. The test results are shown in Table 1 below.

[0060] Accelerated stability testing The functional liposome-encapsulated peptide concentrates prepared in each example and comparative example were dispensed into transparent glass bottles and stored in a constant temperature and humidity chamber at 40±2℃ and 75±5% relative humidity. Samples were taken on day 0 and day 30, and the encapsulation efficiency at each time point was measured according to the encapsulation efficiency determination method described above. The encapsulation efficiency was compared with the initial encapsulation efficiency on day 0, and the retention percentage was calculated. The formula for calculating the encapsulation efficiency retention rate is as follows: Retention rate (%) = Encapsulation rate on day 30 / Encapsulation rate on day 0 × 100% Each sample was measured in triplicate, and the average value was taken. The test results are shown in Table 1 below.

[0061] Anti-precipitation ability test Accurately weigh the centrifuge tubes (W0), then add 10.0 g (W1) of the liposome dispersions prepared in Examples 2-10 and Comparative Examples 1-3, respectively, and record them as Group 2-10 and Group 1-3; centrifuge at 10000 rpm for 30 min; carefully pour out the supernatant, invert the centrifuge tubes on filter paper to drain for 5 min; weigh the total weight of the centrifuge tubes with precipitate (W2), and then calculate the precipitation rate according to the following formula: Sedimentation rate (%) = (W2 - W0) / W1 × 100% Each sample was measured in triplicate, and the average value was taken. The test results are shown in Table 1 below.

[0062] Table 1 Group Initial encapsulation ratio (%) Encapsulation retention rate (%) after 30 days Sedimentation rate (%) Example 2 84.4 78.5 12.3 Example 3 85.0 91.8 2.3 Example 4 84.7 84.4 8.7 Example 5 85.8 92.1 2.0 Example 6 85.4 85.3 8.9 Example 7 86.3 94.8 1.5 Example 8 86.5 94.2 1.8 Example 9 86.0 87.6 7.8 Example 10 87.3 96.5 1.2 Comparative Example 1 85.1 65.2 18.5 Comparative Example 2 84.5 62.8 19.1 Comparative Example 3 82.3 86.0 5.6 As shown in Table 1, comparing Examples 2-10 and Comparative Examples 1-3, the initial encapsulation rates of Examples 5-10 were generally better than those of Examples 2-4 and Comparative Examples 1-3, with Example 10 showing the best initial encapsulation rate at 87.3%. This indicates that the synergistic use of membrane functional agents and stabilizers in the preparation of functional liposome-encapsulated peptides is beneficial to improving the initial encapsulation rate. Furthermore, the synergistic use of cetearyl alcohol polyether-6 olive oil ester as a membrane functional agent and a stabilizer formed by the combination of sclerotium tsulphurein and schistosomes can further improve the initial encapsulation rate of functional liposome-encapsulated peptides. The reason for this may be that cetearyl alcohol polyether-6 olive oil ester significantly improves the encapsulation rate by enhancing membrane fluidity and emulsification properties. At the same time, the combination of sclerotium tsulphurein and schistosomes can effectively stabilize liposomes and overcome the inherent defects of membrane functional agents. Compared to cetearyl alcohol polyether-6 olive oil ester, dipalmitoyl hydroxyproline can increase the rigidity and orderliness of lipid membranes, reduce membrane fluidity, and the higher membrane rigidity may make it easier to generate cracks or leaks during high-pressure homogenization, causing peptides to escape after homogenization.

[0063] Comparing Examples 2-10 with Comparative Examples 1-3, it was found that the encapsulation retention rate of Examples 3, 5, 7, 8, and 10 remained above 90%, and the precipitation rate was all below 2.5%; the encapsulation retention rate of Examples 2, 4, 6, and 9 was below 90%, and the precipitation rate increased to above 7.5%; while the encapsulation retention rate of Comparative Examples 1-2, which lacked effective stabilizers, decreased significantly, and the precipitation rate increased significantly. Among them, Example 10 had the best encapsulation retention rate and the lowest precipitation rate, demonstrating excellent stability and anti-precipitation ability. The reason for this may be that the polyether chains of cetearyl alcohol polyether-6 oleate can insert into and influence the tightly packed lipid bilayer formed by hydrogenated lecithin and phytosterols, thus imparting dynamic stability to the liposomes from within the membrane. This allows the lipid membrane to deform and recover better under thermal stress, effectively preventing peptide leakage. Furthermore, the hydrophilic polyether chains can form a hydration layer on the liposome surface, generating a strong steric repulsion force to prevent liposome aggregation and precipitation. At the same time, sclerotium gum and schistosomein can intertwine in water, forming a continuous and robust three-dimensional hydration gel network on the outside of the liposomes, thereby encapsulating and fixing them within. This steric hindrance prevents liposomes from approaching and aggregating, providing microscopic spatial stability. This not only effectively resists membrane fusion and active substance leakage caused by high temperature and humidity environments but also physically restricts the movement of liposomes, further reducing the leakage of active peptides and enhancing anti-precipitation ability. The extract of *Cladosporium antarctica* primarily provides electrostatic repulsion, but its steric hindrance is weaker than that of sclerotium gum and schistosome extract, resulting in lower encapsulation retention and weaker anti-precipitation ability. Comparative Examples 1 and 2, lacking external stability, experienced rapid aggregation and rupture of liposomes under thermal stress, leading to rapid degradation and leakage of the active ingredient, resulting in poor retention and anti-precipitation ability.

[0064] Test Example 2 In vitro skin permeability test Preparation before the experiment: Fresh, intact detached pigskin with a thickness of 1.0±0.2 mm was prepared as a skin model; the experiment was conducted using a Franz vertical diffusion cell, with an effective diffusion area of ​​approximately 1.77 cm² and a receiving chamber volume of approximately 7 mL; Preparation of receiving solution: Anhydrous ethanol and 0.1 mol / L pH 7.4 PBS buffer were mixed at a volume ratio of 3:7 to prepare the receiving solution; the receiving solution was degassed under magnetic stirring for 30 minutes before the experiment. Sample weighing: Based on 50 μg acetyl hexapeptide-8, the compositions containing 50 μg acetyl hexapeptide-8 prepared in Examples 1-10 and Comparative Examples 1-3 with anti-aging, firming, lifting and wrinkle-reducing effects were accurately weighed as samples and recorded as Examples 1-10 and Comparative Examples 1-3, respectively. Test method: Pigskin was fixed between the supply tank and the receiving chamber, with the keratin layer facing the supply tank. The receiving chamber was filled with receiving liquid, air bubbles were removed, and the water bath temperature was maintained at 37±0.5℃ with a constant magnetic stirring speed. After equilibration for 30 minutes, samples from Examples 1-10 and Comparative Examples 1-3 were placed in the supply tank and received at 37℃. At 2, 4, 8, 12, and 24 hours, 0.5 mL samples were taken from the receiving chamber, and fresh receiving liquid of equal volume and temperature was immediately added. After filtration through a 0.22 μm microporous membrane, the content of acetyl hexapeptide-8 was quantitatively analyzed using LC-MS / MS. The cumulative permeate (Q) was calculated using the following formula. n ): Q n =[C n ×V+Σ(C i ×V s )] / A In the formula, C n C represents the concentration at the nth sampling point. i V represents the concentration at the i-th sampling point; V is the volume of the receiving chamber; V s Where A is the sampling volume and A is the effective diffusion area.

[0065] Table 2 Group 24-hour cumulative skin permeability (μg / cm²) Example 1 0.15±0.03 Example 2 0.72±0.06 Example 3 0.78±0.03 Example 4 0.73±0.05 Example 5 0.88±0.05 Example 6 0.83±0.04 Example 7 0.95±0.06 Example 8 1.12±0.07 Example 9 1.00±0.06 Example 10 1.25±0.05 Comparative Example 1 0.38±0.05 Comparative Example 2 0.33±0.04 Comparative Example 3 0.45±0.03 As shown in Table 2, comparing Examples 1-10 and Comparative Examples 1-3, the 24-hour cumulative permeation of Examples 2-10 was higher than that of Examples 1 and Comparative Examples 1-3. Furthermore, compared to Examples 5-7 which added dipalmitoyl hydroxyproline, Examples 8-10 which added cetearyl alcohol polyether-6 oleate generally exhibited higher permeability, with Example 10 showing the highest 24-hour cumulative permeation, indicating better skin permeability. This may be because the oleic acid in the cetearyl alcohol polyether-6 oleate structure can disrupt the ordered structure of the skin lipid barrier, increasing the fluidity of the stratum corneum. The polyether-6 chain can enhance the hydrophilicity of liposomes and skin hydration, as well as increase the surface activity of liposomes, promoting the fusion of liposomes with the skin epidermis. Cetearyl alcohol can assist the spreading and penetration of liposomes on the skin surface, thus effectively reducing skin barrier resistance, enhancing the flexibility of the liposome membrane, and making acetyl hexapeptide-8 more easily penetrate the stratum corneum. The saturated palmitoyl chain of dipalmitoyl hydroxyproline results in high membrane rigidity, reducing the liposome's ability to fuse with the skin, thus limiting its deformation and penetration on the skin surface and reducing transdermal efficiency. Simultaneously, the synergistic use of sclerotium gum and schistosome extract provides a dual physical and biological pathway for liposome penetration, further enhancing skin permeability. Compared to Examples 2-10, Comparative Examples 1-3 lack stabilizers or membrane function agents, resulting in a lack of external stabilizing protection for the liposomes, making them prone to inactivation on the skin surface and hindering effective delivery of acetyl hexapeptide-8. Therefore, the transdermal yield is significantly lower than that of Examples 2-10.

[0066] Test Example 3 Wrinkle removal effect test 130 healthy female volunteers aged 35-55 with obvious crow's feet were recruited and randomly divided into 13 groups of 10 each. Each group used the anti-aging, firming, lifting and wrinkle-reducing compositions prepared in Examples 1-10 and Comparative Examples 1-3, respectively. The groups were recorded as Examples 1-10 and Comparative Examples 1-3. After cleansing their faces in the morning and evening, the volunteers in each group evenly applied the anti-aging, firming, lifting and wrinkle-reducing compositions prepared in Examples 1-10 and Comparative Examples 1-3 to their entire face, focusing on massaging the corners of their eyes. They used the compositions for 8 consecutive weeks without using any other anti-wrinkle skincare products. Under constant photographic conditions, images of the corners of the eyes were collected using a professional skin image analysis system (VISIA-CRPA) before use (week 0), at 4 weeks of use, and at 8 weeks of use. The changes in wrinkle area (%) and wrinkle depth (mm) after 8 weeks of use were analyzed compared with those before use (week 0). The specific test results are shown in Table 3 below. In the table, "-" indicates a negative value, which represents a decrease. The larger the absolute value, the better the effect.

[0067] Table 3 Group Wrinkle area reduction rate (%) after 8 weeks Wrinkle depth reduction rate (%) after 8 weeks Example 1 -8.5 -7.2 Example 2 -16.5 -15.9 Example 3 -18.2 -17.5 Example 4 -16.8 -15.1 Example 5 -20.1 -18.8 Example 6 -18.9 -17.8 Example 7 -21.3 -20.2 Example 8 -23.1 -22.6 Example 9 -21.5 -20.7 Example 10 -25.8 -24.9 Comparative Example 1 -12.5 -11.0 Comparative Example 2 -11.8 -10.5 Comparative Example 3 -14.2 -13.4 Table 3 shows that the wrinkle-reducing effects of each embodiment differed significantly, with Example 10 showing the best performance, reducing wrinkle area and depth by 25.8% and 24.9% respectively after 8 weeks. This may be because cetearyl alcohol polyether-6 oleate has higher skin permeability, directly converting into more active peptides that reach the target site, resulting in a stronger and faster wrinkle-reducing effect. Simultaneously, the synergistic use of sclerotium gum and schistosome extract provides better physical stability and transdermal penetration enhancement, while schistosome extract helps improve spatial stability and repair the skin base. These three components construct a stable, efficient, and self-contained delivery system, effectively improving the wrinkle-reducing effect of the composition. Compared to Example 10, Example 7, due to the high membrane rigidity caused by dipalmitoyl hydroxyproline, likely resulted in most liposomes remaining only on the skin surface or superficial layer, failing to deliver sufficient acetyl hexapeptide-8 to the muscle layer, thus its effect was inferior to Example 10. Comparative Examples 1 and 2 showed the weakest effects due to the lack of stabilizers, as the liposomes were easily inactivated. Comparative Example 3, although stable, lacked the high-activity peptide delivery efficiency of cetearyl alcohol polyether-6 oleate, resulting in limited efficacy.

Claims

1. A composition with anti-aging, firming, lifting, and wrinkle-reducing effects, characterized in that, Based on a 100g raw material system, it includes: Moisturizer A 5-10g; Moisturizer B 3-8g; Moisturizer C 0.1-0.8g; Moisturizer D 0.1-0.3g; Skin Conditioner A 1-5g; Skin conditioning agent B 0.05-0.5g; Skin conditioning agent C 0.05-0.3g; Anti-wrinkle functional agent A 0.5-2g; Anti-wrinkle functional agent B 0.5-2g; Anti-wrinkle functional agent C 0.5-2g; Emulsifier 0.05-0.2g; Thickener 0.1-0.3g; Preservative 0.05-0.5g; Add water to bring the total weight to 100g; The wrinkle-reducing functional agent C, by mass percentage, is composed of 0.05-0.15% acetyl hexapeptide-8 or functional liposome-encapsulated peptide, 0.03-0.05% ethylhexylglycerin, 2-5% 1,2-hexanediol, and the balance being water.

2. The composition according to claim 1, characterized in that, Moisturizer A is glycerin; moisturizer B, by mass percentage, comprises 28-35% glyceryl triethylhexanoate, 3-6% sorbitol, 0.1-0.8% 1,2-hexanediol, 0.1-0.8% p-hydroxyacetophenone, 0.1-0.2% pentaerythritol tetra(bis-tert-butylhydroxyhydrogenated cinnamic acid) ester, 0.08-0.15% acrylate / C10-30 alkanol acrylate crosspolymer, 0.005-0.015% sodium hydroxide, 0.005-0.015% sodium hyaluronate, and 0.0001-0.001% CI It is composed of 61565, 0.0001-0.001% β-carotene, 0.0001-0.0015% corn (ZEAMAYS) oil, 0.0001-0.0015% tocopherol and the balance water; the moisturizer C is 1,2-hexanediol; the moisturizer D is 1,3-propanediol.

3. The composition according to claim 1, characterized in that, Skin conditioning agent A, by mass percentage, is composed of 3-4% silk fibroin, 1-2% L-hydroxyproline, 1-2% conchiolin powder, and the balance apple fiber; skin conditioning agent B, by mass percentage, is composed of 45-55% saccharide isomers, 3-8% 1,2-pentanediol, and the balance water; skin conditioning agent C is composed of water and glycerol glucoside mixed by mass.

4. The composition according to claim 1, characterized in that, The wrinkle-reducing functional agent A, by mass percentage, is composed of 0.05%-0.15% dipeptide diaminobutyryl benzylamide diacetate, 0.02-0.05% ethylhexylglycerin, 2-5% 1,2-hexanediol, and the balance being water.

5. The composition according to claim 1, characterized in that, The wrinkle-reducing functional agent B, by mass percentage, is composed of 0.001-0.003% arginine / lysine polypeptide, 2-5% 1,2-hexanediol, 0.02-0.05% ethylhexylglycerin, and the balance being water.

6. The composition according to claim 1, characterized in that, The method for preparing the functional liposome-encapsulated peptide includes the following steps: The membrane material and membrane functional agent are added to an organic solvent and mixed and dissolved. The organic solvent is then removed by rotary evaporation to form a uniform lipid film. Active peptides are dissolved in an hydration medium to obtain a peptide-containing aqueous phase, which is then added to the above-mentioned lipid film for hydration to form a crude liposome dispersion. The crude liposome dispersion is then homogenized under high pressure to obtain a liposome core dispersion. Stabilizers were added to the liposome core dispersion for dispersion stabilization treatment, and finally dried to obtain powdered functional liposome-encapsulated peptides.

7. The composition according to claim 6, characterized in that, The preparation method of the functional liposome-encapsulated peptide includes the following steps: S1. Place 3-5g of hydrogenated lecithin, 0.3-0.7g of phytosterol, and 0.1-0.4g of membrane function agent in a round-bottom flask, add 40-60mL of anhydrous ethanol, and place in a water bath at 45-55℃. Mix and dissolve at 150-250rpm. Use a rotary evaporator to evaporate for 20-40min at a water bath temperature of 45-55℃, a rotation speed of 100-150rpm, and a pressure of -0.09MPa to -0.1MPa to completely remove the organic solvent and form a uniform lipid film on the inner wall of the flask. S2. Dissolve 0.5-1.5g of acetyl hexapeptide-8 in 150-250mL of PBS buffer (0.005-0.015mol / L, pH 7.2-7.6) to obtain a peptide-containing aqueous phase. Add the peptide-containing aqueous phase (preheated to 50-60℃) to the above lipid membrane for hydration at 50-60℃, 100-200rpm, and 45-75min to form a crude liposome dispersion. Circulate the crude liposome dispersion through a high-pressure homogenizer at an operating pressure of 12000-18000psi 3-7 times to obtain the liposome core dispersion. S3. Add 0.1-0.5g of stabilizer to the liposome core dispersion at 40-50℃, and mix and stir at 300-500rpm for 45-75min to obtain a functional liposome-encapsulated peptide concentrate; freeze-dry to obtain powdered functional liposome-encapsulated peptides. The membrane functional agent is selected from at least one of cetearyl alcohol polyether-6 olive oil ester and dipalmitoyl hydroxyproline; The stabilizer is selected from one or more combinations of Antarctic spirochetal extract, sclerotium gum, and schistosome extract.

8. The composition according to claim 1, characterized in that, The emulsifier is an acrylic (ester) / C10-30 alkanol acrylate crosspolymer.

9. The composition according to claim 1, characterized in that, The thickener is triethanolamine; the preservative is p-hydroxyacetophenone.

10. A method for preparing the composition according to any one of claims 1-9, characterized in that, Includes the following steps: Step 1: Add water at 75-80℃ and 300-500rpm, accounting for 60-80% of the total water volume. Then add moisturizer A, moisturizer D, and skin conditioning agent A in sequence and mix for 15-25 minutes. Then add moisturizer B and homogenize at 1500-2500rpm for 3-5 minutes to obtain the emulsion base. Step 2: Cool the emulsion matrix to 38-42℃ at 400-600 rpm, and then add wrinkle-reducing functional agent A, wrinkle-reducing functional agent B, wrinkle-reducing functional agent C, skin conditioning agent B and skin conditioning agent C in sequence to obtain a mixed system. In this step, each ingredient should be stirred for 5-10 minutes to ensure that it is evenly mixed before adding the next one. Step 3: Continue stirring until the temperature of the mixed system drops to 35-38℃, then add the emulsifier and continue stirring for 15-25 minutes; then add the thickener and mix until the system thickens instantly and becomes translucent; then add the humectant C and preservative, add the remaining water to 100%, adjust the speed to 200-400 rpm, and stir for 20-30 minutes to obtain the raw material composition; Step 4: Discharge the obtained raw material composition, let it stand and age for 24-48 hours, and then fill it to obtain the finished product composition with anti-aging, firming, lifting and wrinkle-reducing effects.