A composition containing a yeast / rice fermented filtrate having a hair loss prevention effect

Abstract

The application discloses a composition containing yeast / rice fermentation filtrate with anti-hair loss effect. The composition contains yeast / rice fermentation product filtrate, rice fermentation product filtrate, ginger root extract, cattail extract and Asian white birch juice. The application also provides application of the composition in preparation of anti-hair loss hair care products, and a shampoo containing the composition and a preparation method of the shampoo. Experimental results show that the composition can significantly promote hair growth, effectively improve seborrheic alopecia by regulating expressions of hair growth related factors such as KGF, VEGF, TGF-beta 1 and DHT, and the like. The composition has strong synergistic effect of each component, is safe and non-irritating, has simple preparation process, and is suitable for industrial production.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of scalp care and raw material compounding, and particularly relates to a composition containing yeast / rice fermentation filtrate with anti-hair loss effect. BACKGROUND

[0002] Hair loss problems are increasingly common, especially seborrheic alopecia, which is related to many factors such as abnormal androgen levels, hair follicle microcirculation disorders, and inflammatory reactions. The current market anti-hair loss products mostly use chemical drugs (such as minoxidil) or single plant extracts, which have certain effects, but have problems such as large side effects, limited effect, easy recurrence, or single component and difficult multi-target effect. In addition, although there are individual reports of using yeast fermentation products or plant extracts in the prior art, there is a lack of compounding schemes for achieving synergistic effect through scientific proportioning, resulting in unsatisfactory anti-hair loss and hair growth effect.

[0003] Therefore, it is of great significance to develop a safe, efficient, and multi-target natural anti-hair loss composition. The present application provides a composition with significant anti-hair loss and hair growth effect by reasonable proportioning of various natural active ingredients, filling the gap in the prior art. SUMMARY

[0004] In view of the deficiencies in the prior art, the present application aims to provide a composition with significant anti-hair loss and hair growth effect by reasonable proportioning of various natural active ingredients, and its preparation method and application.

[0005] In order to achieve the above-mentioned purpose, the present application discloses the following technical solutions:

[0006] In the first aspect, the present application provides a composition with anti-hair loss effect, which contains yeast / rice fermentation product filtrate, rice fermentation product filtrate, ginger root extract, typha orientalis extract, and asian white birch juice.

[0007] Preferably, the composition contains the following components by mass fraction:

[0008] Yeast / rice fermentation product filtrate 10-20 parts;

[0009] Rice fermentation product filtrate 7-11 parts;

[0010] Ginger root extract 1-5 parts;

[0011] Typha orientalis extract 2-4 parts;

[0012] Asian white birch juice 5-7 parts.

[0013] In the second aspect, the present application provides the use of the composition of the first aspect in the preparation of hair care products with anti-hair loss and hair growth effect.

[0014] In a third aspect, the present application provides a shampoo with anti-hair loss effect, wherein the shampoo comprises the composition of the first aspect.

[0015] Preferably, the composition is added to the shampoo in an amount of 1-2 wt%.

[0016] Further preferably, the shampoo further comprises a conditioning agent, a surfactant, a thickening agent, an emulsifier, a humectant, an antioxidant, a stabilizer, a bacteriostatic agent, a pearling agent, a fragrance, a chelating agent, an anti-dandruff agent, a pH regulator and a solvent.

[0017] Further preferably, the conditioning agent is at least one of biota leaf extract, rice bran oil, radix falatiae extract, rice extract, hydrolyzed rice protein ginseng root extract, soapberry extract, common wheat seed extract, malt extract, millet seed extract, rice hull extract, hydrolyzed silk, sclerotia of sclerotia fungus extract;

[0018] Further preferably, the surfactant is at least one of sodium laureth sulfate, cocamidopropyl betaine, cocamide DEA, ammonium lauryl sulfate, polyquaternium-10, cocamide MEA, polyquaternium-7;

[0019] Further preferably, the humectant is at least one of butylene glycol, glycerin, 1,2-hexanediol, panthenol, allantoin, 1,3-propanediol, caprylyl glycol;

[0020] Further preferably, the antioxidant is at least one of hydroxypropyl tetrahydropyran triol, ergothioneine;

[0021] Further preferably, the bacteriostatic agent is at least one of phenoxyethanol, sodium benzoate, dichlorobenzyl alcohol, p-hydroxyacetophenone, caprylhydroxamid acid;

[0022] Further preferably, the thickening agent is at least one of guar hydroxypropyltrimonium chloride, carbomer, xanthan gum, carrageenan, gellan gum.

[0023] Further preferably, the solvent is water; the emulsifier is cetostearyl alcohol; the stabilizer is sodium chloride; the pearling agent is ethylene glycol distearate; the chelating agent is disodium EDTA; the anti-dandruff agent is piroctone olamine salt; the pH regulator is sodium hydroxide.

[0024] In a fourth aspect, the present application provides a preparation method of the shampoo of the third aspect, wherein the preparation method comprises the following steps:

[0025] The composition, conditioner, surfactant, thickening agent, emulsifier, humectant, antioxidant, stabilizer, bacteriostatic agent, pearl agent, essence, chelating agent, anti-dandruff agent, pH regulator and solvent are mixed uniformly to obtain the shampoo.

[0026] Advantages of the present application:

[0027] 1. The present application promotes hair growth and improves the environment of hair follicles by scientific compounding of yeast / rice fermentation product filtrate, rice fermentation product filtrate, ginger root extract, typha orientalis extract and asiatic birch juice. Animal experiments show that the length and weight of mouse hair in the treatment group are significantly higher than those in the model group, and the effect is better than that in the single component control group.

[0028] 2. The composition provided by the present application can significantly up-regulate the expression of hair growth promoting factors KGF and VEGF, and down-regulate the level of inhibitory factors TGF-β1 and DHT, thereby effectively improving the hair follicle cycle and promoting hair growth.

[0029] 3. The shampoo provided by the present application significantly improves the hair loss reduction rate and hair density growth rate of the subjects after 8 weeks of use, and no adverse reactions such as redness and itching occur during the experiment, so the safety is high.

[0030] 4. The preparation method of the shampoo provided by the present application is simple and easy to operate, the raw materials are easy to obtain, and the method is suitable for large-scale industrial production, so the shampoo has good market application prospect. DETAILED DESCRIPTION

[0031] Those skilled in the art can improve the process parameters according to the content herein. It should be particularly pointed out that all similar replacements and changes are obvious to those skilled in the art, and they are considered to be included in the present application.

[0032] The products and methods of the present application have been described by preferred embodiments, and relevant personnel can obviously modify or appropriately change and combine the products and methods described herein without departing from the content, spirit and scope of the present application, to realize and apply the present application technology.

[0033] I. In the present application

[0034] Yeast / rice fermentation product filtrate: purchased from Guizhou Keyi Chuang Biotechnology Co., Ltd.;

[0035] Rice fermentation product filtrate: purchased from Ganzhou Bai Zhen Tang Spice Co., Ltd.;

[0036] Ginger root extract: purchased from Yunnan Weicai Biotechnology Co., Ltd.;

[0037] Typha orientalis extract: purchased from Guangzhou Baiyao Biotechnology Co., Ltd.;

[0038] Asian birch juice: purchased from Guangzhou Liyan Biotechnology Co., Ltd.

[0039] The remaining raw materials can be obtained on the market.

[0040] II. Composition with anti-falling effect

[0041] The composition with anti-falling effect is prepared by compounding the raw materials according to the mass ratio in Table 1.

[0042] Table 1 Mass ratio of raw materials

[0043] Raw material name Composition 1 Composition 2 Composition 3 Yeast / rice fermentation product filtrate 10 15 20 Rice fermentation product filtrate 7 9 11 Ginger root extract 1 3 5 Typha orientalis extract 2 3 4 Asian white birch sap 5 6 7

[0044] III. Performance test

[0045] On the basis of the composition 2 formula, single component control groups 1-5 are set up, and the composition 2 component ratio is yeast / rice fermentation product filtrate: rice fermentation product filtrate: ginger root extract: Typha orientalis extract: Asian birch juice = 15:9:3:3:6. The following single component control group addition concentrations can be obtained by conversion.

[0046] Table 2 Experimental grouping

[0047]

[0048] Note: The solvent is obtained by compounding water and glycerol at a mass ratio of 1:1.

[0049] 1. Experimental materials

[0050] 1.1 Experimental animals

[0051] SPF male C57BL / 6 mice, 6-8 weeks old, weighing 20-22 g, purchased from Beijing Vantoll Life Science and Technology Co., Ltd., and raised in an environment with a temperature of 22-25°C and a relative humidity of 50%-60%, with a 12h light-dark cycle, free access to food and water, and adapted for 1 week before the experiment.

[0052] 1.2 Test samples

[0053] Composition 1, 2, 3: compounded according to Table 1 formula, then diluted with solvent (water-glycerol, mass ratio 1:1) to a concentration of 1%;

[0054] Single component control groups 1-5: prepared according to the concentration in Table 2 experimental grouping (control group 1: 0.42% yeast / rice fermentation product filtrate; control group 2: 0.25% rice fermentation product filtrate; control group 3: 0.084% ginger root extract; control group 4: 0.084% Typha orientalis extract; control group 5: 0.17% Asian birch juice), and the solvent is the same as before;

[0055] Positive control: 2% minoxidil tincture;

[0056] Modeling reagent: testosterone (dissolved in anhydrous ethanol and then physiological saline was added to prepare a 5 mg / mL solution);

[0057] Hair removal reagent: rosin-paraffin mixture (mass ratio 1:1, used after heating and melting).

[0058] 1.3 Test reagent

[0059] Mouse KGF ELISA kit, mouse VEGF ELISA kit, mouse TGF-β1 ELISA kit, mouse DHT ELISA kit.

[0060] 2 Experimental method

[0061] 2.1 Animal modeling

[0062] Hair removal: mark a 2 cm x 3 cm experimental area on the back of the mouse with picric acid, evenly apply the melted rosin-paraffin mixture to the area, and after cooling and hardening, remove it to remove the hair in the experimental area; confirm that the skin in the experimental area is smooth and undamaged, which means that the hair removal is successful.

[0063] Modeling: except for the blank control group, the remaining groups of mice were applied with 5 mg / mL testosterone solution in the depilated area, 0.1 mL each time, twice a day, for 19 consecutive days to establish a model of seborrheic alopecia; the blank control group was applied with an equal amount of physiological saline instead of testosterone.

[0064] 2.2 Grouping and administration

[0065] The successfully depilated mice were randomly divided into 11 groups, 8 mice in each group, and the grouping is shown in Table 2. After modeling, administration began, and all test samples were applied to the depilated area on the back of the mice, 0.1 mL each time, once a day, for 19 consecutive days; during the administration period, the mice were normally fed, and the skin condition (no redness, itching, and other irritation) was observed and recorded.

[0066] 2.3 Detection index and operation

[0067] 2.3.1 Detection of mouse hair growth

[0068] Hair length measurement: on the 14th day and the 19th day of administration, 5 mice were randomly selected from each group, and 10 newly born hairs were randomly pulled out in the experimental area on the back, the length of each hair was measured with a vernier caliper, and the average length of each group was calculated (retaining 2 decimal places).

[0069] Hair weight measurement: On the 19th day of administration (end of experiment), all the newborn hairs on the experimental area of the back of each mouse were carefully collected and weighed using a one-hundredth electronic balance. The total weight of the hairs in each group was recorded and the average weight was calculated (to one decimal place).

[0070] 2.3.2 Concentration detection of mouse skin hair growth related factors

[0071] Sample preparation: On the 19th day of administration, the mice were sacrificed by removing the vertebrae, and the skin tissue on the experimental area of the back was taken. Physiological saline pre-cooled at 4°C was added, and a homogenate was prepared using a tissue homogenizer. Centrifugation was performed at 3000 r / min for 10 min, and the supernatant was taken, aliquoted, and stored at -80°C for testing.

[0072] ELISA detection: Strictly according to the instructions of the ELISA kit for each factor, the absorbance value was determined using an enzyme-labeled analyzer, and the mass concentration of KGF, VEGF, TGF-β1, and DHT in the skin tissue was calculated according to the standard curve (unit: μg / L). The data for each group was recorded.

[0073] 3 Experimental results

[0074] 3.1 Results of mouse hair growth indicators

[0075] Table 3 Results of detection of newborn hair length and weight of mice )

[0076] Experimental group Hair length on day 14 (mm) Hair length on day 19 (mm) Hair weight on day 19 (mg) Blank control group 3.92±0.38** 7.35±0.29** 46.2±3.4** Model group 2.61±0.52 6.15±0.21 31.5±5.1 Positive control group (minoxidil) 3.55±0.26** 7.01±0.18** 45.8±3.7** Composition 1 (1%) 3.28±0.31** 6.68±0.15** 42.3±4.2** Composition 2 (1%) 3.53±0.42** 6.95±0.12** 46.5±3.9** Composition 3 (1%) 3.35±0.29** 6.72±0.16** 43.1±4.5** Single ingredient control group 1 (yeast / rice) 2.65±0.49 6.20±0.23 32.8±4.9 Single ingredient control group 2 (rice fermentation) 2.62±0.51 6.16±0.22 31.9±5.0 Single ingredient control group 3 (ginger root extract) 3.05±0.32* 6.42±0.19* 35.6±4.5* Single ingredient control group 4 (Typha orientalis) 2.61±0.53 6.18±0.24 32.5±5.2 Single ingredient control group 5 (Asian white birch sap) 2.60±0.50 6.15±0.20 31.9±4.8

[0077] Note: * indicates a significant difference compared with the model group, P<0.05, ** P<0.01.

[0078] 3.2 Results of mouse skin hair growth related factor concentration

[0079] Table 4 Results of detection of mouse skin hair growth related factor concentration , unit: μg / L)

[0080] Experimental group KGF concentration VEGF concentration TGF-β1 concentration DHT concentration Blank control group 1335±162** 1382±145** 97±12** 68±11** Model group 592±72 741±83 273±33 243±26 Positive control group (minoxidil) 1298±138** 1135±136** 145±21** 165±21** Composition 1 (1%) 1056±112** 982±121** 195±18** 188±22** Composition 2 (1%) 1285±123** 1152±118** 208±22** 158±18** Composition 3 (1%) 1082±105** 995±131** 198±24** 192±23** Single ingredient control group 1 (yeast / rice) 601±79 753±86 244±43* 232±38 Single ingredient control group 2 (rice fermentation) 596±81 748±91 268±32 237±34 Single ingredient control group 3 (ginger root extract) 782±92* 895±101* 225±37* 220±29* Single ingredient control group 4 (Typha orientalis) 612±78 766±88 241±38* 241±37 Single ingredient control group 5 (Asian white birch sap) 608±73 743±90 267±42 239±84

[0081] Note: * indicates a significant difference compared with the model group, P<0.05, ** P<0.01.

[0082] 3.3 Analysis of results

[0083] As can be seen from the data in Tables 3 and 4, the compositions 1, 2 and 3 of the present application can significantly improve the hair growth condition of the seborrheic alopecia model mice. Compared with the model group, the hair length and weight of the mice in the composition treatment groups are significantly increased (P<0.01), and the composition 2 has the best effect, with the hair length reaching 4.53 mm on the 14th day and the hair weight reaching 46.5 mg on the 19th day, close to the positive control minoxidil group. In terms of the hair growth related factors, the compositions treatment groups can significantly increase the concentrations of KGF and VEGF (P<0.01) and decrease the concentrations of TGF-β1 and DHT (P<0.01), indicating that they promote hair growth by regulating the hair follicle microenvironment through multiple pathways. The effects of the single component control groups are not as good as those of the composition groups, confirming that there is a synergistic effect among the components.

[0084] Four, preparation of a shampoo with anti-hair loss effect

[0085] Step 1: Add deionized water to a stirring container, start stirring at a speed of 200 rpm, and heat to 75-80°C. Then add EDTA disodium, sodium laureth sulfate, cocamidopropyl betaine, butylene glycol, glycerol, 1,2-hexanediol, panthenol, allantoin and sodium chloride in sequence, and stir until completely dissolved to obtain mixture A, which is kept at temperature for 15 min and ready for use;

[0086] Step 2: Add cetylstearyl alcohol, ethylene glycol distearate and rice bran oil to another container, and heat in a 75-80°C water bath to melt and clarify the raw materials to obtain mixture B, which is kept at temperature for 15 min;

[0087] Step 3: Add mixture B to mixture A, homogenize at 5000 r / min for 3-5 min, then keep stirring at 150 r / min and keep at temperature for 10 min to obtain mixture C;

[0088] Step 4: Reduce the temperature of mixture C to 45-50°C, add guar hydroxypropyltrimonium chloride and carbomer, stir at 200 r / min for 20 min, then add piroctone olamine, p-hydroxyacetophenone, octanohydroxamic acid, rice extract, hydrolyzed silk, polyquaternium-10, composition 2, antioxidant and fragrance in sequence, stir at 300 r / min for 30 min, finally adjust the pH value to 5.5-6.5 with sodium hydroxide, filter through a 100-mesh filter bag, and then fill to obtain the shampoo with anti-hair loss effect.

[0089] Table 5: Mass percentage of shampoo raw materials (unit: %)

[0090]

[0091] Five, efficacy evaluation of application examples

[0092] 1 Subject

[0093] Select 18-50 years old healthy people, diagnosed with dandruff, a total of 50 people, each group of 10 people, nearly 3 months without using any anti-hair loss products, no effect on hair growth of drugs, no inflammation, eczema, wound or allergic history, liver and kidney function, voluntarily signed informed consent, can complete the 8-week experiment and follow-up.

[0094] 2 Exclusion criteria

[0095] (1) Pregnant women, lactating women or people in the preparatory period; (2) Allergic to any component of shampoo; (3) Suffering from thyroid disease, anemia and other systemic diseases, or in the postpartum, postoperative hair loss period; (4) Unable to ensure regular work and rest during the experiment (such as long-term overtime) or frequent hair perming; (5) Other conditions of the experiment.

[0096] 3 Grouping

[0097] Group Grouping Blank control Shampoo without "Composition 2" (other ingredients same as application example) Positive control Commercially available shampoo containing 1% minoxidil Application example 1 According to the formulation of application example 1 in Table 5 Application example 2 According to the formulation of application example 2 in Table 5 Application example 3 According to the formulation of application example 3 in Table 5

[0098] 4 Usage

[0099] Each group of subjects uses the corresponding shampoo once a day, with a dosage of about 5mL, and is prohibited from using other anti-hair loss, hair growth or anti-dandruff products during the experiment. Keep washing your hair once a day, avoid spicy food and overtime.

[0100] Evaluation time points: baseline period (0 weeks) - before the experiment begins, collect basic data for all evaluation indicators; endpoint (8 weeks): evaluate the differences in anti-hair loss and hair growth indicators from baseline.

[0101] 5 Evaluation indicators and detection methods

[0102] 5.1 Anti-hair loss effect evaluation

[0103] Hair loss count: before washing, use a dry towel to dry your hair, use a special filter to collect the hair loss when washing, and count the hair loss after drying;

[0104] Hair loss count: use a standard nylon comb (2mm tooth spacing) to comb the scalp in the fixed area for 10 times, collect the hair loss and count;

[0105] Combine the above hair loss numbers, record the data, and calculate the growth rate compared to the baseline.

[0106] 5.2 Hair growth effect evaluation

[0107] Hair density measurement: use a dermatoscope to take pictures of the fixed area on the top of the head, use image analysis software to count the number of hairs per unit area (1cm 2 ) and calculate the growth rate compared to the baseline.

[0108] 6 Experimental results

[0109] Table 6 Results of hair loss prevention test

[0110] Group 8-week hair loss reduction rate / % 8-week hair density growth rate / % Blank control -2.3 1.3 Positive control -35.6 12.0 Application example 1 -18.9 6.9 Application example 2 -29.5 10.2 Application example 3 -31.3 13.0

[0111] 7 Results analysis

[0112] From the data in Table 6, after using the shampoo containing composition 2 for 8 weeks, the hair loss reduction rates of application examples 1, 2 and 3 were -18.9%, -29.5% and -31.3% respectively, and the hair density growth rates were 6.9%, 10.2% and 13.0% respectively, all of which were better than the blank control group, and the effect of application example 3 was comparable to that of the positive control group. This shows that the shampoo of the present application can effectively reduce hair loss and promote hair growth, and the effect is improved with the increase of the amount of composition added. No adverse reactions were reported during the experiment, indicating that the product is safe and reliable.

[0113] Six, summary

[0114] In summary, the composition of the present application can effectively improve seborrheic alopecia by regulating the hair growth cycle at multiple targets through the synergistic effect of multiple natural active ingredients. The shampoo prepared thereby not only has a significant effect of preventing hair loss and promoting hair growth, but also is mild and safe, suitable for long-term use, and has a wide application value.

[0115] Although the specific embodiments of the present application are described above, those skilled in the art should understand that this is only an example, and the protection scope of the present application is defined by the appended claims. Those skilled in the art can make various changes or modifications to these embodiments without departing from the principles and essence of the present application, and such changes and modifications fall within the protection scope of the present application.

Claims

1. A composition having anti-hair loss effect, characterized in that, The composition comprises, by weight parts, the following components: 10-20 parts of yeast / rice fermentation product filtrate; 7-11 parts of rice fermentation product filtrate; 1-5 parts of ginger root extract; Oriental cattail extract 2-4 parts; 5-7 parts of Asian birch sap.

2. The use of the composition according to claim 1 in the preparation of hair care products with anti-hair loss and hair growth effects.

3. A shampoo with anti-hair loss properties, characterized in that, The shampoo contains the composition of claim 1.

4. The shampoo according to claim 3, characterized in that, The composition is added to the shampoo in an amount of 1-2 wt%.

5. The shampoo according to claim 4, characterized in that, The shampoo also contains conditioning agents, surfactants, thickeners, emulsifiers, moisturizers, antioxidants, stabilizers, antibacterial agents, pearlescent agents, fragrances, chelating agents, anti-dandruff agents, pH adjusters, and solvents. The surfactant is at least one of sodium lauryl ether sulfate, cocamidopropyl betaine, cocamidopropyl betaine, ammonium lauryl sulfate, polyquaternium salt-10, cocamidopropyl betaine, and polyquaternium salt-7. The emulsifier is cetearyl alcohol.

6. The shampoo according to claim 5, characterized in that, The conditioning agent is at least one of the following: Platycladus orientalis leaf extract, rice bran oil, Polygonum multiflorum extract, rice extract, hydrolyzed rice protein, ginseng root extract, Sapindus mukorossi extract, common wheat seed extract, malt extract, millet seed extract, rice husk extract, hydrolyzed silk, and Tuberculia nigricans extract. The moisturizer is at least one selected from butylene glycol, glycerin, 1,2-hexanediol, panthenol, allantoin, 1,3-propanediol, and caprylyl glycol. The antioxidant is at least one of hydroxypropyltetrahydropyrantriol and ergothioneine; The antibacterial agent is at least one of phenoxyethanol, sodium benzoate, dichlorobenzyl alcohol, p-hydroxyacetophenone, and octanoyl hydroxamic acid; The thickener is at least one of guar hydroxypropyltrimethylammonium chloride, carbomer, xanthan gum, carrageenan, and gellan gum.

7. A method for preparing the shampoo according to claim 5 or 6, characterized in that, The preparation method includes the following steps: The shampoo is obtained by mixing the aforementioned composition, conditioning agent, surfactant, thickener, emulsifier, moisturizer, antioxidant, stabilizer, antibacterial agent, pearlescent agent, fragrance, chelating agent, anti-dandruff agent, pH adjuster, and solvent evenly.

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