Composition with effects of removing dandruff, inhibiting bacteria and relieving itching as well as preparation method and application of composition

Abstract

The invention discloses a composition with effects of removing dandruff, inhibiting bacteria and relieving itching as well as a preparation method and application thereof, and belongs to the technical field of daily chemicals. The composition is compounded with a yeast fermentation lysate filtrate, a bacillus fermentation product, a fructus kochiae extract, a red clover extract and a cacumen biotae extract; through a'micro-ecology-barrier-inflammation 'triple synergistic path, the synergistic interaction of inhibiting bacteria more quickly, relieving itching for a longer time and removing dandruff more thoroughly is realized. The relative contents of the substances are optimized, so that the efficacy is maximized, and the stability of the composition is ensured. The composition can be used as a cosmetic raw material to be applied to scalp stability maintaining essence, has a good effect of improving the scalp microenvironment, and is mild and non-irritant.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of daily chemicals, and particularly relates to a composition with the effects of dandruff removal, bacterium inhibition and itch alleviation, a preparation method and application thereof. BACKGROUND

[0002] The core demands of Chinese consumers for scalp health management are focused on the improvement of dandruff, sebum and itch problems, which directly promotes the market dominance of dandruff and itch relieving shampoo products. Malassezia, as a lipophilic fungus, is identified as the core pathogen causing pathological symptoms such as dandruff and scalp itching. This group lacks the ability to synthesize endogenous fatty acids, but can hydrolyze host sebum by secreting lipase, phospholipase and sphingomyelinase, and decompose lipids such as triglycerides into free fatty acids for its metabolic proliferation. In this process, irritating metabolites are continuously produced, including unsaturated fatty acids (such as oleic acid), free fatty acids and indole derivatives; directly damage the tight junction structure of the stratum corneum, destroy the skin physical barrier; interfere with the normal differentiation program of sebaceous gland cells; induce abnormal proliferation and accelerated metabolism of keratinocytes; cause a vicious cycle mechanism of feedback hypersecretion of sebum, neurogenic inflammation-mediated itching, and deterioration of hair follicle microenvironment leading to dandruff. Based on this pathological model, the dual intervention strategy of simultaneously targeting Malassezia proliferation inhibition and inflammation signal pathway blockade constitutes the key technical path to solve the dandruff-itching problem.

[0003] The chemical efficacy ingredients (such as zinc pyrithione (ZPT), piroctone olamine (OCT) and other chemical synthetic substances) used in mainstream shampoo products at home and abroad have hidden dangers of sensitization and irritation, and long-term or high-concentration use can easily lead to damage to the scalp barrier, imbalance of flora, and even induce the defect of drug resistance. Although the concept of "plant dandruff" is rising driven by the concept of environmental friendliness, most of the products on the market still use the pseudo-natural formula framework of "chemical dandruff agent as the main component + trace amount of plant additives"; single plant extract (matrine, hydrolyzed chitin, soapnut, pinus massoniana and the like) has high safety, but it is difficult to reach an effective bacteriostatic concentration in a short rinse system due to low solubility, poor stability and insufficient scalp penetration rate, and the efficacy persistence is poor. The multi-component complex system is often based on empirical compatibility, lacks verification of the synergistic mechanism against pathogenic bacteria such as Malassezia, resulting in narrow bacteriostatic spectrum, large fluctuation in itch relieving effect, and easy precipitation or inactivation with surfactants, fragrances and other basic formulations. Some natural or probiotic raw materials (such as lactic acid bacteria fermentation products, plant polysaccharides and the like) can improve the microecology, but have insufficient immediate performance for dandruff and itch relief, and are difficult to be industrialized due to strict requirements for pH and preservative system.

[0004] Chinese patent CN105412530B discloses a traditional Chinese medicine extract with antibacterial and dandruff-removing effects, and a preparation method and application thereof. The effective components are prepared from the following raw materials by alcohol extraction: 40-60 parts of walnut, 150-250 parts of apricot kernel, 150-250 parts of wax gourd seed, and 30-60 parts of winter melon. Although the raw material components are natural, the effect is slow.

[0005] Chinese patent CN112315835A discloses an oil-controlling and dandruff-removing composition. The composition comprises zinc pyrithione, melaleuca alternifolia oil (tea tree oil), a sugar isomer, octanoyl glycine, and eucalyptus oil. The composition has the effects of dandruff removal and oil control, but does not have the effects of anti-inflammatory and itching relief. Moreover, the composition still contains chemical effective components.

[0006] The defects of the prior art collectively result in the difficulty of simultaneously achieving the effects of "dandruff removal, itching relief, and antibacterial" in commercially available shampoos. Either the antibacterial effect is strong but the irritation is great, or the composition is mild and safe but the effect is slow and the cycle is long. Therefore, it has become a technical problem to be solved in the field of shampoos to develop a composition that has the effects of high-efficiency antibacterial, rapid itching relief, mildness, no irritation, and stability. SUMMARY

[0007] To solve the above technical problems, the present application provides a composition with the effects of dandruff removal, antibacterial, and itching relief. The composition comprises yeast fermentation lysate filtrate, bacillus fermentation product, and fructus tomatencis extract.

[0008] Further, in the composition with the effects of dandruff removal, antibacterial, and itching relief, the mass ratio of the yeast fermentation lysate filtrate, the bacillus fermentation product, and the fructus tomatencis extract is 2-4.5:1.5-3.5:0.3-2.

[0009] Preferably, the mass ratio of the yeast fermentation lysate filtrate, the bacillus fermentation product, and the fructus tomatencis extract is 2.8-3.5:2.0-3.0:0.7-1.5.

[0010] Further, the composition further comprises extract of red clover and extract of biota orientalis.

[0011] Further, in the composition, the mass ratio of the yeast fermentation lysate filtrate, the extract of red clover, and the extract of biota orientalis is 2.8-3.5:1.0-2.0:0.3-1.5.

[0012] The present application also provides a preparation method of the composition. In one embodiment, the composition is obtained by mixing the yeast fermentation lysate filtrate, the bacillus fermentation product, the fructus tomatencis extract, the extract of red clover, and the extract of biota orientalis and then fully stirring. The above raw materials can be commercially available or self-prepared.

[0013] In an embodiment, the composition is prepared by simultaneous fermentation of the Ganoderma lucidum, Saccharomycete and Bacillus subtilis on the Semen Astragali, Trifolium pratense and Platycladus orientalis.

[0014] Preferably, the Saccharomycete is Debaryomyces hansenii, and the Bacillus subtilis is Bacillus subtilis.

[0015] Further, the fermentation step comprises:

[0016] S1, strain activation

[0017] The Ganoderma lucidum, Saccharomycete and Bacillus subtilis are mixed in a ratio of 1:1-2:1, and inoculated in a liquid culture medium in a total inoculum of 0.1-1%, and cultured at a temperature of 28-30°C for 24-78h;

[0018] S2, raw material pretreatment

[0019] The Semen Astragali fruits, Trifolium pratense whole grass and Platycladus orientalis are washed and dried, and ground to 40-80 mesh; the Semen Astragali, Trifolium pratense and Platycladus orientalis powders are mixed in a mass ratio of 0.7-1.5:1.0-2.0:0.3-1.5, and the mixed powders are mixed with distilled water in a mass ratio of 1-3:1;

[0020] S3, solid state fermentation

[0021] The mixture of S2 is mixed uniformly with sterilized corn flour, and then placed in a fermentation barrel, and inoculated with the activated strain liquid for solid aerobic fermentation, with a fermentation time of 20-25 days, a fermentation temperature of 26-32°C, and a fermentation humidity of 70-87%;

[0022] The mass ratio of the mixture of S2 to the sterilized corn flour to the activated strain liquid is 1-2:1, and the mass ratio of the mixture of S2 to the sterilized corn flour is 1:1-3;

[0023] S4, the fermented product of S3 is crushed, placed at 80°C for 2h, and then rapidly frozen to 5°C; then the extraction liquid is added under stirring, and the extraction is continued for 2h, and then the mixture is allowed to stand and filtered, and the filtrate is collected and concentrated at low temperature to obtain the composition.

[0024] Further, the present application also provides a cosmetic raw material, which comprises the composition, a solvent and a preservative.

[0025] Preferably, in terms of mass, the cosmetic raw material comprises 85-95% of the composition, 0.5-2% of the preservative and the balance of the solvent.

[0026] Further, the solvent includes but is not limited to one or more of water, glycerol, propylene glycol, butylene glycol and pentylene glycol; preferably, the solvent includes glycerol.

[0027] Furthermore, the preservatives include, but are not limited to, one or more of p-hydroxyacetophenone, 1,2-hexanediol, octyl glycol, pentylene glycol, ethylhexylglycerin, and capryloyl hydroxamic acid.

[0028] Furthermore, the preservatives are 0.3-1% p-hydroxyacetophenone and 0.2-1% 1,2-hexanediol.

[0029] Furthermore, the present invention also provides a scalp-stabilizing essence, the ingredients of which include the aforementioned cosmetic raw materials. Specifically, by mass fraction, the ingredients of the scalp-stabilizing essence include: 0.1-0.5% ammonium acryloyldimethyl taurate / VP copolymer, 0.05-0.3% allantoin, 3-7% methyl propylene glycol, 0.5-2% preservative, 1-10% of the aforementioned cosmetic raw materials, 0.1-0.7% emulsifier, 2-8% alcohol solvent, and the balance being water.

[0030] Furthermore, the emulsifier includes, but is not limited to, one or more of PEG-40 hydrogenated castor oil, PEG-50 hydrogenated castor oil, Tween-20, Tween-40, alkyl glycosides, and fatty acid polyethylene glycol esters; preferably, PEG-40 hydrogenated castor oil.

[0031] Furthermore, the scalp stabilizing essence also includes 0.5-3% plant extracts, wherein the plant extracts are composed of Scutellaria baicalensis root extract, Edelweiss extract and citrus peel extract in a mass ratio of 3-7:2-8:2-8.

[0032] Furthermore, the scalp stabilizing essence also includes 0.2% plant-based anti-dandruff agents.

[0033] Furthermore, the scalp-stabilizing essence may also contain 0.05-3% of auxiliary agents, including but not limited to one or more of fragrances, pigments, surfactants, chelating agents, pH adjusters, and film-forming agents. In one embodiment, the scalp-stabilizing essence further includes 0.05-1% fragrance.

[0034] The beneficial effects of this invention are:

[0035] (1) This application provides a composition with anti-dandruff, antibacterial, and antipruritic effects, which is compounded with yeast fermentation lysate filtrate, Bacillus fermentation product, Kochia scoparia extract, red clover extract, and Platycladus orientalis leaf extract. Through a triple synergistic pathway of "microecology-barrier-inflammation", it achieves synergistic effects of faster antibacterial action, longer-lasting antipruritic effect, and more thorough dandruff removal. The relative content of the above substances is further optimized to maximize efficacy while ensuring the stability of the composition.

[0036] (2) The present application uses solid-state aerobic fermentation to prepare the composition, and uses multiple enzymatic hydrolysis of Aureobacterium tumefaciens, yeast and Bacillus to ferment the substrate, which promotes the generation of more active ingredients and secondary metabolites, which is conducive to maximizing the efficacy of the composition.

[0037] (3) The cosmetic raw materials and scalp stabilizing essence prepared using the composition of this application are mild and non-irritating, with excellent antibacterial and antimicrobial properties, which can fundamentally improve and stabilize the scalp environment. Detailed Implementation

[0038] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0039] This invention provides a composition with anti-dandruff, antibacterial and antipruritic effects, comprising yeast fermentation lysate filtrate, Bacillus fermentation product and Kochia scoparia extract.

[0040] Yeast fermentation lysate filtrate

[0041] Yeast fermentation lysate filtrate is a liquid product obtained by fermenting a specific yeast strain, lysing the yeast cells using physical or chemical methods, and then filtering to remove solid components. Because it is rich in active metabolic small molecules such as amino acids, polypeptides, nucleic acids, organic acids, vitamins, and cell wall components such as β-glucan and mannan oligosaccharides, it has good efficacy in antibacterial, antipruritic, and anti-dandruff effects. Its main mechanism of action is as follows: yeast lysate provides nutrients for beneficial bacteria resident on the skin (such as Staphylococcus epidermidis), promoting their proliferation and thus competitively inhibiting pathogenic bacteria (such as Malassezia, Staphylococcus aureus, and Propionibacterium acnes); its antimicrobial peptides bind to the cell membrane of microorganisms through electrostatic interaction, forming transmembrane pores that lead to leakage of contents and thus destroy the cell membrane integrity of pathogens; the small molecule peptides can also downregulate the expression of pro-inflammatory cytokines such as IL-6 and TNF-α, relieving inflammatory itching caused by microorganisms or irritants; the organic acids produced by yeast fermentation (such as lactic acid and succinic acid) can lower the pH of the skin surface, creating a microenvironment unfavorable to the survival of pathogens; all of the above regulate the scalp microenvironment, effectively inhibit scalp pathogens, promote the normal differentiation of scalp keratinocytes, and reduce dandruff accumulation.

[0042] Bacillus fermentation product

[0043] Bacillus fermentation products also have excellent antibacterial, antipruritic, and anti-dandruff effects, mainly relying on their secondary metabolites. Their lipopeptide compounds (such as surfactants, itursin, and fentanyl) can insert into the cell membranes of pathogens (such as Malassezia and Staphylococcus aureus), causing leakage of cell contents, thereby inhibiting or killing microorganisms. Fentanyl can also interfere with the biofilm structure of Malassezia, reducing its adhesion and reproductive capacity, thus reducing dandruff production at the source. Its extracellular polysaccharides, amino acids, organic acids, enzymes, and other metabolites can inhibit the release of inflammatory factors, repair the skin barrier, reduce the level of the stress hormone cortisol, relieve itching, gently promote keratin renewal, and reduce scale accumulation.

[0044] Poria cocos extract

[0045] Kochia scoparia extract is rich in triterpenoid saponins, flavonoids, and alkaloids, which can exert antibacterial effects by disrupting the cell structure and inhibiting enzyme activity. Flavonoids and saponins can not only promote the repair and regeneration of keratinocytes, restore the skin barrier, reduce the activation of nerve endings by external stimuli, and promote skin repair, but also inhibit the expression of inflammatory factors and inhibit histamine release, thus relieving allergic itching.

[0046] Furthermore, in the composition having anti-dandruff, antibacterial and antipruritic effects, the mass ratio of yeast fermentation lysate filtrate, Bacillus fermentation product and Kochia scoparia extract is 2-4.5:1.5-3.5:0.3-2.

[0047] This application combines yeast fermentation lysate filtrate, Bacillus fermentation product, and Kochia scoparia extract. This is not a simple additive process, but rather achieves a synergistic effect of antibacterial, antipruritic, and anti-dandruff action through a triple synergistic pathway of "microecology-barrier-inflammation." At the antibacterial level, the yeast fermentation lysate filtrate inhibits the survival space of Malassezia, while the Bacillus fermentation product directly disrupts the cell membrane of Malassezia, rapidly killing bacteria. Kochia scoparia extract interferes with the metabolic enzyme activity of Malassezia through triterpenoid saponins, blocking hyphal extension and spore formation. These three components form an antibacterial closed loop of "inhibition-killing-blocking," resulting in a significantly higher antibacterial rate than any single ingredient used alone. In terms of itch relief, yeast fermentation lysate filtrate repairs the stratum corneum and reduces moisture loss by replenishing NMF (natural moisturizing factor) and B vitamins. Beta-glucan in Bacillus subtilis products activates Langerhans cells, enhances skin immunity, and reduces the release of inflammatory factors. Flavonoids in Kochia scoparia extract inhibit histamine release, quickly relieving neurogenic pruritus. These three components form a closed loop of "barrier repair → inflammation suppression → itch relief," significantly increasing the itch-relieving speed. In terms of dandruff removal, yeast fermentation lysate filtrate adjusts pH through organic acids, reducing the colonization environment for Malassezia. Bacillus subtilis products gently exfoliate dead skin cells and reduce scale buildup through the synergistic effect of proteases and lipopeptides. Kochia scoparia extract promotes normal differentiation of keratinocytes through saponins, reducing abnormal dandruff. The synergistic effect of these three components significantly improves dandruff removal efficiency without causing dependence. Therefore, the combination of yeast filtrate, Bacillus product, and Kochia scoparia extract in this application achieves a triple closed loop of "microecology-barrier-inflammation," resulting in faster antibacterial action, longer-lasting itch relief, and more thorough dandruff removal. Further optimization of the relative content of the three components maximizes efficacy while ensuring the stability of the composition.

[0048] Furthermore, the composition having anti-dandruff, antibacterial and antipruritic effects comprises yeast fermentation lysate filtrate, Bacillus fermentation product and Kochia scoparia extract, in a mass ratio of 2.8-3.5: 2.0-3.0: 0.7-1.5.

[0049] Furthermore, the composition also includes red clover (TRIFOLIUM PRATENSE) extract and cypress (BIOTA ORIENTALIS) leaf extract. Red clover extract is a natural raw material from plants of the genus Trifolium in the legume family, widely used in cosmetics, health products, and pharmaceuticals. Its isoflavone components can bidirectionally regulate estrogen levels, alleviate menopausal hot flashes and mood swings, and improve female hormone-induced hair loss. The phenolic acids and polyphenols, volatile oils, and flavonoid glycosides in the active ingredients can scavenge free radicals, inhibit inflammatory factors (such as IL-6 and TNF-α), and alleviate skin inflammation, eczema, and scalp sensitivity. The volatile oils, flavonoids, quercetin, and tannins in the cypress leaf extract can promote dermal papillary cell proliferation, improve microcirculation, dilate capillaries around hair follicles, enhance local nutrient delivery, and have antibacterial and anti-inflammatory effects.

[0050] Furthermore, the mass ratio of yeast fermentation lysate filtrate, red clover extract, and arborvitae leaf extract in the composition is 2.8-3.5:1.0-2.0:0.3-1.5. Excessive use of red clover extract will inhibit yeast activity and affect the antibacterial effect, while excessive use of arborvitae leaf extract will negatively impact the composition's odor and stability.

[0051] This application also provides a method for preparing the composition. In one embodiment, the composition is obtained by thoroughly mixing and stirring a mixture of yeast fermentation lysate filtrate, Bacillus fermentation product, Kochia scoparia extract, Trifolium repens extract, and Platycladus orientalis leaf extract. The above raw materials can be commercially available or prepared in-house.

[0052] In one embodiment, the composition is prepared by simultaneous fermentation of Kochia scoparia, Trifolium repens, and Platycladus orientalis leaves by Mycorrhiza uralensis, yeast, and Bacillus subtilis. Preferably, the yeast is Saccharomyces hansenii, and the Bacillus subtilis is Bacillus subtilis. Specific fermentation steps include:

[0053] S1, strain activation

[0054] Mix Aureobacterium tumefaciens, yeast and Bacillus in a ratio of 1:1 to 2:1, and inoculate a total of 0.1-1% into liquid culture medium for culture at a temperature of 28-30℃ for 24-78 hours.

[0055] The liquid culture medium formula is as follows: 3g yeast powder, 10g peptone, 10g sucrose, 15g soluble starch, 5g sodium acetate, 2g triammonium citrate, 0.15g K2HPO4, 0.5g MnSO4, 0.2g MgSO4, and 1mL Tween-80 dissolved in 1L distilled water, and sterilized at 121℃ under high temperature and high pressure.

[0056] The *Eurotium cristatum* strain is identified as *Eurotium cristatum*, strain number: CICC 2099, from the China Industrial Microbial Culture Collection Center.

[0057] The yeast strain is *Debaryomyceshansenii*, strain number: CICC1266, China Industrial Microbial Culture Collection Center.

[0058] The Bacillus species mentioned is Bacillus subtilis, strain number: CICC 10275, China Industrial Microbial Culture Collection Center.

[0059] S2, Raw material pretreatment

[0060] Wash and dry the fruit of Kochia scoparia, the whole herb of Trifolium repens, and the leaves of Platycladus orientalis, and then pulverize them to 40-80 mesh. Mix the powders of Kochia scoparia, Trifolium repens, and Platycladus orientalis in a mass ratio of 0.7-1.5:1.0-2.0:0.3-1.5, and then mix the mixed powder with distilled water in a mass ratio of 1-3:1.

[0061] S3, solid-state fermentation

[0062] After mixing S2 with sterilized corn flour evenly, place it in a fermentation tank, add activated inoculum liquid, and carry out solid-state aerobic fermentation. The fermentation time is 20-25 days, the fermentation temperature is 26-32℃, and the fermentation humidity is 70-87%.

[0063] The mass ratio of the total mass of the S2 mixture and sterilized corn flour to the mass of the activated bacterial liquid is 1-2:1; the mass ratio of the S2 mixture to sterilized corn flour is 1:1-3.

[0064] S4. After crushing the product from S3 fermentation, place it at 80°C for 2 hours and then rapidly freeze it to 5°C. Then, add the extract while stirring and continue extraction for 2 hours. After standing and filtering, take the filtrate and concentrate it at low temperature to obtain the composition.

[0065] The extract consisted of 0.1 mol / L phosphate buffer (pH around 7), and the mass ratio of the extract to the fermented product was 10:1.

[0066] In one embodiment, S2 is as follows: after cleaning the fruit of Kochia scoparia, dry it at 50-60°C and pulverize it to 40-80 mesh; dry the whole plant of Trifolium repens and pulverize it to 60-80 mesh; wash the leaves of Platycladus orientalis, freeze-dry them and grind them to 60-80 mesh; mix the powders of Kochia scoparia, Trifolium repens and Platycladus orientalis, and mix them with distilled water at a mass ratio of 1-3:1.

[0067] In one embodiment, S3 is as follows: the mixture from S2 is thoroughly mixed with sterilized corn flour and placed in a fermentation tank. Activated inoculum liquid is added for solid-state aerobic fermentation. During days 0-7, the fermentation temperature is 26-28°C, and the humidity is 85-87%, with the pile turned every 48 hours. During days 8-18, the fermentation temperature is 30-32°C, and the humidity is 75-80%, with the pile turned three times daily. During days 19-25, the fermentation temperature is reduced to 26-28°C, and the humidity is reduced to 70-75%. After thoroughly mixing the S2 mixture with sterilized corn flour, place it in a fermentation tank and add the activated microbial inoculum liquid for solid-state aerobic fermentation. During days 0-7, maintain a fermentation temperature of 26-28℃ and humidity of 85-87%, turning the mixture every 48 hours. During days 8-18, maintain a fermentation temperature of 30-32℃ and humidity of 75-80%, turning the mixture three times daily. During days 19-25, reduce the fermentation temperature to 26-28℃ and humidity to 70-75%, turning the mixture three times daily. The total mass ratio of the S2 mixture and sterilized corn flour to the activated microbial inoculum liquid is 1:1; the mass ratio of the S2 mixture to the sterilized corn flour is also 1:1.

[0068] Furthermore, the present invention also provides a cosmetic raw material, comprising: the aforementioned composition, a solvent, and a preservative. Preferably, by weight, the cosmetic raw material comprises: 85-95% of the aforementioned composition, 0.5-2% of a preservative, and the balance being a solvent.

[0069] Furthermore, the solvent includes, but is not limited to, one or more of water, glycerol, propylene glycol, butylene glycol, and pentanediol; preferably, it includes glycerol.

[0070] Furthermore, the preservatives include, but are not limited to, one or more of p-hydroxyacetophenone, 1,2-hexanediol, octyl glycol, pentylene glycol, ethylhexylglycerin, and capryloyl hydroxamic acid.

[0071] Furthermore, the preservatives are 0.3-1% p-hydroxyacetophenone and 0.2-1% 1,2-hexanediol. The p-hydroxyacetophenone structure itself has antibacterial properties, which can delay the oxidative discoloration of peptides and flavonoids in yeast / Bacillus fermentation products, while also inhibiting the oxidation and odor production of arborvitae leaf volatile oil. 1,2-hexanediol can reduce the risk of yeast polysaccharide flocculation at high temperatures. Controlling the relative content allows both to work synergistically, improving the high and low temperature cycling stability of the composition. Compared to traditional preservatives, these ingredients are virtually non-irritating to the scalp, resulting in a better user experience.

[0072] Furthermore, the present invention also provides a scalp-stabilizing essence, the ingredients of which include the aforementioned composition. Specifically, by mass fraction, the ingredients of the scalp-stabilizing essence include: 0.1-0.5% ammonium acryloyldimethyl taurate / VP copolymer, 0.05-0.3% allantoin, 3-7% methyl propylene glycol, 0.5-2% preservative, 1-10% of the aforementioned composition, 0.1-0.7% emulsifier, 2-8% alcohol solvent, and the balance being water.

[0073] Furthermore, the preservative is ketone and 1,2-hexanediol in a mass ratio of 1:1-2.

[0074] Furthermore, the emulsifier includes, but is not limited to, one or more of PEG-40 hydrogenated castor oil, PEG-50 hydrogenated castor oil, Tween-20, Tween-40, alkyl glycosides, and fatty acid polyethylene glycol esters; preferably, PEG-40 hydrogenated castor oil.

[0075] Furthermore, the alcohol solvent is selected from one or more of ethanol, isopropanol, propylene glycol, butanediol, pentanediol, hexanediol, glycerol, and sorbitol; preferably ethanol.

[0076] Furthermore, the scalp-stabilizing essence also includes 0.5-3% plant extracts, wherein the plant extract components are Scutellaria baicalensis root extract, Edelweiss extract, and citrus peel extract in a mass ratio of 3-7:2-8:2-8. Preferably, the mass ratio of Scutellaria baicalensis root extract, Edelweiss extract, and citrus peel extract is 5:4:4. The plant extracts can be obtained by mixing commercially available Scutellaria baicalensis root extract, Edelweiss extract, and citrus peel extract, or can be prepared according to the method described in Chinese Patent Application No. 202510177340.X.

[0077] Furthermore, the scalp-stabilizing essence also includes 0.2-1.5% of a plant-based anti-dandruff agent. Based on the total mass of the plant-based anti-dandruff agent, the ingredients include 10-25% Sophora flavescens root extract, 10-25% Magnolia officinalis bark extract, 5-18% Patchouli extract, 5-18% Chamomile extract, and butylene glycol to bring the balance to 100%.

[0078] In one embodiment, the plant-based dandruff remover, by weight, comprises 13-18% Sophora flavescens root extract, 13-18% Magnolia officinalis bark extract, 10-15% Pogostemon cablin extract, 10-15% Chamomile chrysanthemum extract, and butylene glycol to make up the balance to 100%.

[0079] Furthermore, the scalp-stabilizing essence may also contain 0.05-3% of auxiliary agents, including but not limited to one or more of fragrances, pigments, surfactants, chelating agents, pH adjusters, and film-forming agents. In one embodiment, the scalp-stabilizing essence further includes 0.05-1% fragrance.

[0080] Furthermore, the preparation method of the scalp-stabilizing essence is as follows:

[0081] (1) Methylpropanediol and acetone are mixed to form phase D. Phase D is preheated to about 60-75℃ and completely dissolved. Emulsifier, additives and alcohol solvent are mixed to form phase F and completely dissolved.

[0082] (2) Water is added to the emulsification pot as phase A, then phase B (acryloyl dimethyl taurate ammonium / VP copolymer) and phase C (allantoin material) are added. The temperature is raised to about 80-85℃, stirred until completely dissolved, and homogenized for 3-5 minutes until the material is uniform. After maintaining the temperature for 20 minutes, the temperature is lowered by stirring.

[0083] (3) Cool down to about 68-75℃, add the pre-dissolved D phase, and stir to disperse evenly;

[0084] (4) Cool down to about 40-50℃, add the F phase material and the remaining components as the E phase, and stir to disperse evenly;

[0085] (5) Cool down to about 35-38℃, stop stirring, take a sample for testing, and filter the material out through a 400-mesh filter cloth after it passes the test.

[0086] Example 1

[0087] This embodiment provides a composition with anti-dandruff, antibacterial and antipruritic effects, comprising yeast fermentation lysate filtrate, Bacillus fermentation product, Kochia scoparia extract, red clover extract and Platycladus orientalis leaf extract, in a mass ratio of 3.38:2.5:1.0:1.5:1.0.

[0088] The composition is prepared by:

[0089] S1, strain activation

[0090] A mixture of Aureobacterium tumefaciens, yeast, and Bacillus was inoculated into liquid culture medium at a ratio of 1:1:1, with a total inoculation amount of 1%. The mixture was cultured at 28°C for 48 hours.

[0091] The liquid culture medium formula is as follows: 3g yeast powder, 10g peptone, 10g sucrose, 15g soluble starch, 5g sodium acetate, 2g triammonium citrate, 0.15g K2HPO4, 0.5g MnSO4, 0.2g MgSO4, and 1mL Tween-80 dissolved in 1L distilled water, and sterilized at 121℃ under high temperature and high pressure.

[0092] The *Eurotium cristatum* strain is identified as *Eurotium cristatum*, strain number: CICC 2099, from the China Industrial Microbial Culture Collection Center.

[0093] The yeast strain is *Debaryomyceshansenii*, strain number: CICC1266, China Industrial Microbial Culture Collection Center.

[0094] The Bacillus species mentioned is Bacillus subtilis, strain number: CICC 10275, China Industrial Microbial Culture Collection Center.

[0095] S2, Raw material pretreatment

[0096] After cleaning the fruit of Kochia scoparia, dry it at 50℃ and grind it to 60 mesh; dry the whole plant of Trifolium repens and grind it to 60 mesh; clean the leaves of Platycladus orientalis, freeze-dry them and grind them to 80 mesh; mix the powders of Kochia scoparia, Trifolium repens and Platycladus orientalis in a mass ratio of 1.0:1.5:1.0, and then mix the mixed powder with distilled water in a mass ratio of 2:1.

[0097] S3, solid-state fermentation

[0098] After thoroughly mixing the S2 mixture with sterilized corn flour, place it in a fermentation tank. Add the activated inoculum liquid for solid-state aerobic fermentation. During days 0-7, maintain a fermentation temperature of 26-28℃ and a humidity of 85-87%, turning the mixture every 48 hours. During days 8-18, maintain a fermentation temperature of 30-32℃ and a humidity of 75-78%, turning the mixture three times daily. During days 19-25, reduce the fermentation temperature to 26-28℃ and the humidity to 73-75%, turning the mixture three times daily.

[0099] The fermentation temperature is maintained at 26-28℃ and the humidity at 85-87%, with the pile turned every 48 hours. From day 8 to 18, the fermentation temperature is maintained at 30-32℃ and the humidity at 78-80%, with the pile turned three times daily. From day 19 to 25, the fermentation temperature is reduced to 26-28℃ and the humidity to 70-72%, with the pile turned three times daily. The total mass ratio of the S2 mixture and sterilized corn flour to the activated microbial liquid is 1:1; the mass ratio of the S2 mixture to the sterilized corn flour is also 1:1.

[0100] S4. After crushing the product from S3 fermentation, place it at 80°C for 2 hours and then rapidly freeze it to 5°C. Then, add the extract while stirring and continue extraction for 2 hours. After standing and filtering, take the filtrate and concentrate it at low temperature to obtain the composition.

[0101] The extract consisted of 0.1 mol / L phosphate buffer (pH around 7), and the mass ratio of the extract to the fermented product was 10:1.

[0102] Example 2

[0103] This embodiment provides a composition with anti-dandruff, antibacterial and antipruritic effects, comprising yeast fermentation lysate filtrate, Bacillus fermentation product, Kochia scoparia extract, red clover extract and Platycladus orientalis leaf extract, in a mass ratio of 2.8:2.0:0.7:2.0:1.5.

[0104] The composition is prepared by:

[0105] S1, strain activation

[0106] A mixture of Aureobacterium tumefaciens, yeast, and Bacillus was inoculated into liquid culture medium at a ratio of 1:1:1, with a total inoculum of 0.5%, and cultured at 30°C for 24 hours.

[0107] The liquid culture medium formula is as follows: 3g yeast powder, 10g peptone, 10g sucrose, 15g soluble starch, 5g sodium acetate, 2g triammonium citrate, 0.15g K2HPO4, 0.5g MnSO4, 0.2g MgSO4, and 1mL Tween-80 dissolved in 1L distilled water, and sterilized at 121℃ under high temperature and high pressure.

[0108] The *Eurotium cristatum* strain is identified as *Eurotium cristatum*, strain number: CICC 2099, from the China Industrial Microbial Culture Collection Center.

[0109] The yeast strain is *Debaryomyceshansenii*, strain number: CICC1266, China Industrial Microbial Culture Collection Center.

[0110] The Bacillus species mentioned is Bacillus subtilis, strain number: CICC 10275, China Industrial Microbial Culture Collection Center.

[0111] S2, Raw material pretreatment

[0112] After cleaning the fruit of Kochia scoparia, dry it at 60℃ and grind it to 40 mesh; dry the whole plant of Trifolium repens and grind it to 80 mesh; clean the leaves of Platycladus orientalis, freeze-dry them and grind them to 60 mesh; mix the powders of Kochia scoparia, Trifolium repens and Platycladus orientalis in a mass ratio of 0.7:2.0:1.5, and then mix the mixed powder with distilled water in a mass ratio of 1:1.

[0113] S3, solid-state fermentation

[0114] After thoroughly mixing the S2 mixture with sterilized corn flour, place it in a fermentation tank. Add the activated inoculum liquid for solid-state aerobic fermentation. During days 0-7, maintain a fermentation temperature of 27-28℃ and humidity of 85-87%, turning the mixture every 48 hours. During days 8-18, maintain a fermentation temperature of 30-32℃ and humidity of 79-80%, turning the mixture three times daily. During days 19-25, reduce the fermentation temperature to 27-28℃ and humidity to 74-75%, turning the mixture three times daily.

[0115] The fermentation temperature is maintained at 26-28℃ and the humidity at 85-87%, with the pile turned every 48 hours. From day 8 to 18, the fermentation temperature is maintained at 30-32℃ and the humidity at 75-76%, with the pile turned three times daily. From day 19 to 25, the fermentation temperature is reduced to 26-28℃ and the humidity to 70-71%, with the pile turned three times daily. The total mass ratio of the S2 mixture and sterilized corn flour to the activated inoculum liquid is 1:1; the mass ratio of the S2 mixture to the sterilized corn flour is also 1:1.

[0116] S4. After crushing the product from S3 fermentation, place it at 80°C for 2 hours and then rapidly freeze it to 5°C. Then, add the extract while stirring and continue extraction for 2 hours. After standing and filtering, take the filtrate and concentrate it at low temperature to obtain the composition.

[0117] The extract consisted of 0.1 mol / L phosphate buffer (pH around 7), and the mass ratio of the extract to the fermented product was 10:1.

[0118] Example 3

[0119] This embodiment provides a composition with anti-dandruff, antibacterial and antipruritic effects, comprising yeast fermentation lysate filtrate, Bacillus fermentation product, Kochia scoparia extract, red clover extract and Platycladus orientalis leaf extract, in a mass ratio of 3.5:3.0:1.5:1.0:0.3.

[0120] The composition is prepared by:

[0121] S1, strain activation

[0122] A mixture of Aureobacterium tumefaciens, yeast, and Bacillus was inoculated into liquid culture medium at a ratio of 1:1:1, with a total inoculation amount of 1%. The mixture was cultured at 28°C for 48 hours.

[0123] The liquid culture medium formula is as follows: 3g yeast powder, 10g peptone, 10g sucrose, 15g soluble starch, 5g sodium acetate, 2g triammonium citrate, 0.15g K2HPO4, 0.5g MnSO4, 0.2g MgSO4, and 1mL Tween-80 dissolved in 1L distilled water, and sterilized at 121℃ under high temperature and high pressure.

[0124] The *Eurotium cristatum* strain is identified as *Eurotium cristatum*, strain number: CICC 2099, from the China Industrial Microbial Culture Collection Center.

[0125] The yeast strain is *Debaryomyceshansenii*, strain number: CICC1266, China Industrial Microbial Culture Collection Center.

[0126] The Bacillus species mentioned is Bacillus subtilis, strain number: CICC 10275, China Industrial Microbial Culture Collection Center.

[0127] S2, Raw material pretreatment

[0128] After cleaning the fruit of Kochia scoparia, dry it at 50℃ and grind it to 60 mesh; dry the whole plant of Trifolium repens and grind it to 60 mesh; clean the leaves of Platycladus orientalis, freeze-dry them and grind them to 80 mesh; mix the powders of Kochia scoparia, Trifolium repens and Platycladus orientalis in a mass ratio of 1.5:1.0:0.3, and then mix the mixed powder with distilled water in a mass ratio of 2:1.

[0129] S3, solid-state fermentation

[0130] After thoroughly mixing the S2 mixture with sterilized corn flour, place it in a fermentation tank. Add the activated inoculum liquid for solid-state aerobic fermentation. During days 0-7, maintain a fermentation temperature of 26-28℃ and a humidity of 85-87%, turning the mixture every 48 hours. During days 8-18, maintain a fermentation temperature of 30-32℃ and a humidity of 75-78%, turning the mixture three times daily. During days 19-25, reduce the fermentation temperature to 26-28℃ and the humidity to 73-75%, turning the mixture three times daily.

[0131] The fermentation temperature is maintained at 26-28℃ and the humidity at 85-87%, with the pile turned every 48 hours. From day 8 to 18, the fermentation temperature is maintained at 30-32℃ and the humidity at 78-80%, with the pile turned three times daily. From day 19 to 25, the fermentation temperature is reduced to 26-28℃ and the humidity to 70-72%, with the pile turned three times daily. The total mass ratio of the S2 mixture and sterilized corn flour to the activated microbial liquid is 1:1; the mass ratio of the S2 mixture to the sterilized corn flour is also 1:1.

[0132] S4. After crushing the product from S3 fermentation, place it at 80°C for 2 hours and then rapidly freeze it to 5°C. Then, add the extract while stirring and continue extraction for 2 hours. After standing and filtering, take the filtrate and concentrate it at low temperature to obtain the composition.

[0133] The extract consisted of 0.1 mol / L phosphate buffer (pH around 7), and the mass ratio of the extract to the fermented product was 10:1.

[0134] Example 4

[0135] This embodiment provides a cosmetic ingredient, which, by weight, consists of: 93.8% of the composition of Example 1, 0.6% of p-hydroxyacetophenone, 0.6% of 1,2-hexanediol, and the balance being glycerin.

[0136] The preparation method of the cosmetic raw material is as follows: mix the composition, p-hydroxyacetophenone, 1,2-hexanediol and glycerin evenly.

[0137] Example 5

[0138] This embodiment provides a cosmetic ingredient, which, by weight, consists of: 90.5% of the composition of Example 1, 0.3% of p-hydroxyacetophenone, 0.9% of 1,2-hexanediol, and the balance being glycerin.

[0139] The preparation method of the cosmetic raw material is as follows: mix the composition, p-hydroxyacetophenone, 1,2-hexanediol and glycerin evenly.

[0140] Example 6

[0141] This embodiment provides a cosmetic ingredient, which, by weight, consists of: 85.2% of the composition of Example 1, 0.5% of p-hydroxyacetophenone, 0.2% of 1,2-hexanediol, and the balance being glycerin.

[0142] The preparation method of the cosmetic raw material is as follows: mix the composition, p-hydroxyacetophenone, 1,2-hexanediol and glycerin evenly.

[0143] Example 7

[0144] This embodiment provides a scalp stabilizing essence. By mass fraction, the ingredients of the scalp stabilizing essence include: 0.2% ammonium acryloyl dimethyl taurate / VP copolymer, 0.2% allantoin, 5% methyl propylene glycol, 1% preservative, 3% cosmetic raw material of Example 4, 0.3% PEG-40 hydrogenated castor oil, 5% ethanol, 1% plant extract, 1% plant anti-dandruff agent, 0.1% fragrance, and the balance being water.

[0145] The preservative is ketone and 1,2-hexanediol in a mass ratio of 1:1.

[0146] The plant extract comprises Scutellaria baicalensis root extract, Edelweiss extract, and citrus peel extract in a mass ratio of 5:4:4. The plant extract is prepared according to the method described in Chinese Patent Application No. 202510177340.X, hereinafter the same.

[0147] Based on the total mass of the plant-based dandruff remover, the ingredients of the plant-based dandruff remover include 15% Sophora flavescens root extract, 15% Magnolia officinalis bark extract, 12.5% ​​Pogostemon cablin extract, 12.5% ​​Chamomile extract, and butylene glycol to make up the balance to 100%.

[0148] The preparation method of the scalp stabilizing essence is as follows:

[0149] (1) Methylpropanediol and acetone are mixed to form phase D. Phase D is preheated to about 70°C and completely dissolved. Emulsifier, additives and alcohol solvent are mixed to form phase F and completely dissolved.

[0150] (2) Water is added to the emulsification pot as phase A, then phase B (acryloyl dimethyl taurate ammonium / VP copolymer) and phase C (allantoin material) are added. The temperature is raised to about 82°C, stirred until completely dissolved, and homogenized for 5 minutes until the material is uniform. After maintaining the temperature for 20 minutes, the mixture is stirred and cooled.

[0151] (3) Cool down to about 70°C, add the pre-dissolved D phase, and stir to disperse evenly;

[0152] (4) Cool down to about 45°C, add the F phase material and the remaining components as the E phase, and stir to disperse evenly;

[0153] (5) Cool down to about 38℃, stop stirring, take a sample for testing, and filter the material out through a 400-mesh filter cloth after it passes the test.

[0154] Comparative Example 1

[0155] This comparative example provides a composition with anti-dandruff, antibacterial, and antipruritic effects. The components include yeast fermentation lysate filtrate, Bacillus fermentation product, Kochia scoparia extract, and Platycladus orientalis leaf extract, in a mass ratio of 3.38:2.5:1.0:1.0, respectively. Red clover is not added in the preparation method; otherwise, it remains consistent with Example 1.

[0156] Comparative Example 2

[0157] This comparative example provides a composition with anti-dandruff, antibacterial and antipruritic effects. The composition includes yeast fermentation lysate filtrate, Bacillus fermentation product, red clover extract and arborvitae leaf extract, with a mass ratio of 3.38:2.5:1.5:1.0. The preparation method does not include Kochia scoparia fruit, and other aspects are consistent with Example 1.

[0158] Comparative Example 3

[0159] This comparative example provides a composition with anti-dandruff, antibacterial and antipruritic effects. The composition includes yeast fermentation lysate filtrate, Kochia scoparia extract, red clover extract and Platycladus orientalis leaf extract, with a mass ratio of 3.38:1.0:1.5:1.0, respectively. Bacillus is not added in the preparation method, and other aspects are consistent with Example 1.

[0160] Comparative Example 4

[0161] This comparative example provides a method for preparing a composition with anti-dandruff, antibacterial and antipruritic effects, specifically: mixing commercially available yeast fermentation lysate filtrate, Bacillus fermentation product, Kochia scoparia extract, red clover extract and Platycladus orientalis leaf extract in a mass ratio of 3.38:2.5:1.0:1.5:1.0.

[0162] Comparative Example 5

[0163] This comparative example provides a cosmetic ingredient composition, which is: 93.8% of the composition of Example 1, 1.2% of capryloyl hydroxamic acid, and the balance glycerin.

[0164] Performance testing methods:

[0165] I. Stability Testing:

[0166] (1) High temperature and low temperature stability: The samples were placed in constant temperature incubators at 48℃, 5℃ and 25℃ respectively. After the samples were restored to room temperature on the 3rd, 7th, 14th, 21st and 30th days respectively, the color and appearance of the samples and whether the solution had any defects such as fading, layering or precipitation were observed, and the pH was monitored.

[0167] (2) Stability of hot and cold cycles: The hot and cold cycles are carried out in two-day cycles. The solution is placed in a -18℃ constant temperature chamber for the first day and in a 48℃ constant temperature chamber for the last day. After the solution is restored to room temperature, the appearance of the solution is observed and the pH is monitored for any changes. A total of five cycles are monitored.

[0168] The stability test results of the composition and cosmetic raw materials are shown in Table 1.

[0169] Table 1

[0170]

[0171] The results show that the compositions and cosmetic ingredients in the embodiments of this application have excellent stability, which is more conducive to the product shelf life.

[0172] II. Anti-dandruff efficacy test

[0173] The test was conducted according to the "Antimicrobial Test of Anti-dandruff Products against Malassezia (Inhibition Loop Method)":

[0174] (1) Preparation of antibacterial tablets: For liquid antibacterial test samples, take sterile dry filter paper tablets. For liquid products, add an appropriate amount of antibacterial test sample solution to each tablet; for relatively viscous semi-solid products, directly wet the filter paper tablets into the sample. Then, lay the filter paper tablets flat in a clean sterile Petri dish to dry before use.

[0175] (2) Preparation of control sample: Take sterile dry filter paper, add an appropriate amount of sterile distilled water to each paper, and dry it for later use.

[0176] (3) Inoculation of test bacteria: Dip a sterile cotton swab into the suspension of test bacteria and spread it evenly on the surface of the culture medium plate three times. Cover the plate and let it dry at room temperature.

[0177] (4) Placement of Antibacterial Test Samples: For each test, use one contaminated plate, placing both the test sample and the control sample on each plate. Use sterile forceps to place the sample onto the plate surface, ensuring a distance of at least 25 mm between the centers of each sample and at least 5 mm from the periphery of the plate. After placement, gently press the sample with sterile forceps to ensure it adheres tightly to the plate surface. Cover the plate and incubate at 30℃ for 3–5 days, then observe the results. Measure and record the diameter of the inhibition zone (including the sample) using calipers. Repeat the test three times.

[0178] (5) Result determination: The average diameters of the antibacterial rings in the three independent repeated tests were 8.54 mm, 8.73 mm, and 8.14 mm, respectively. It was determined that the scalp stabilizing essence product of Example 7 of this application has the effect of removing dandruff.

[0179] III. Antipruritic effect

[0180] Consumer use testing methods were adopted, and the testing was conducted in accordance with the T / TDCA006-2022 Guidelines for Consumer Use Testing of Cosmetics - Questionnaire Survey Evaluation.

[0181] This experiment included 31 participants (minimum age: 25 years, maximum age: 40 years, mean age: 32 years, male ratio: 9.68%, female ratio: 90.32%). No participants dropped out during the test, and the valid data are from the 31 participants. The trial period was 14 days. Self-comparison was conducted before and after the test. Instructions for use of the scalp stabilizing essence: After wetting hair, apply an appropriate amount to the hair and scalp, and gently massage.

[0182] Results: After 14 days of use, there were significant differences in the subjects' scalp itching, refreshing feeling and scalp soothing degree, indicating that the product has a certain anti-itch and soothing effect.

[0183] Compared to baseline, 93.55% of participants reported that the product relieved itching; 100% of participants indicated that the product had a gentle texture, leaving their scalp feeling refreshed and soothed after use, resulting in a positive experience. No adverse reactions such as dry skin, itching, breakouts, stinging, itch, or burning were observed during the testing process.

[0184] IV. Corrosion Resistance Test

[0185] (1) Microbial detection

[0186] The tests were conducted in accordance with the General Principles of Microbial Testing Methods, Total Colony Count, and Test Methods for Molds and Yeasts in Chapter 5 of the "Cosmetic Safety Technical Specifications" (2015 Edition).

[0187] The test results of the scalp stabilizing essence in the example are shown in Table 2.

[0188] Table 2

[0189] Test item Unit Test result Limit value Total number of colonies CFU / g ＜10 ≤1000 Mould and yeast CFU / g ＜10 ≤100

[0190] As shown in Table 2, the total number of colonies, molds and yeasts all meet the requirements of the "Cosmetic Safety Technical Specifications" (2015 edition).

[0191] (2) Preservative challenge test: According to the Technical Guidelines for Evaluation of Preservative Challenge Test of Cosmetics (China National Institutes for Food and Drug Control, 2024 Edition), 6.4 Preservative challenge test of test sample.

[0192] The test results of the scalp stabilizing essence in the example are shown in Table 3.

[0193] Table 3

[0194]

[0195]

[0196] Wherein, NI indicates that the difference between the technical data results and the previous time point does not exceed 0.5log; "-" indicates that no testing is required. Based on the above tests, judgment criteria and results, it can be determined that the scalp stabilizing essence of this application can effectively prevent the proliferation of microorganisms that may pose a potential safety risk to consumers, and the essence has passed the preservative challenge test.

[0197] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0198] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A composition having anti-dandruff, antibacterial, and antipruritic effects, characterized in that, The ingredients include yeast fermentation lysate filtrate, Bacillus fermentation product, and Kochia scoparia extract.

2. The composition with anti-dandruff, antibacterial, and antipruritic effects according to claim 1, characterized in that, The mass ratio of yeast fermentation lysate filtrate, Bacillus fermentation product and Kochia scoparia extract was 2-4.5:1.5-3.5:0.3-2.

3. The composition with anti-dandruff, antibacterial, and antipruritic effects according to claim 1, characterized in that, The composition also includes red clover extract and arborvitae leaf extract.

4. The composition with anti-dandruff, antibacterial, and antipruritic effects according to claim 1, characterized in that, The mass ratio of yeast fermentation lysate filtrate, red clover extract, and arborvitae leaf extract in the composition is 2.8-3.5:1.0-2.0:0.3-1.

5.

5. A method for preparing the composition according to any one of claims 1-4, characterized in that, The composition is prepared by simultaneous fermentation of Kochia scoparia, Trifolium repens, and Platycladus orientalis leaves by Mycorrhiza uralensis, yeast, and Bacillus subtilis; the yeast is Saccharomyces hansenii, and the Bacillus subtilis is Bacillus subtilis.

6. A cosmetic ingredient comprising the composition according to any one of claims 1-4, characterized in that, By weight, the cosmetic raw material composition is: 85-95% of the composition, 0.5-2% of preservatives and the balance being solvent.

7. The cosmetic raw material according to claim 6, characterized in that, Solvents include one or more of water, glycerol, propylene glycol, butylene glycol, and pentanediol; preservatives include one or more of p-hydroxyacetophenone, 1,2-hexanediol, octyl glycol, pentanediol, ethylhexylglycerin, and capryloyl hydroxamic acid.

8. A scalp-stabilizing essence comprising the cosmetic ingredients according to any one of claims 6-7, characterized in that, By mass fraction, the ingredients of the scalp stabilizing essence include: 0.1-0.5% ammonium acryloyl dimethyl taurate / VP copolymer, 0.05-0.3% allantoin, 3-7% methyl propylene glycol, 0.5-2% preservatives, 1-10% of the cosmetic raw materials, 0.1-0.7% emulsifier, 2-8% alcohol solvents, and the balance being water.

9. The scalp-stabilizing essence according to claim 8, characterized in that, The emulsifier includes one or more of PEG-40 hydrogenated castor oil, PEG-50 hydrogenated castor oil, Tween-20, Tween-40, alkyl glycosides, and fatty acid polyethylene glycol esters.

10. The scalp-stabilizing essence according to claim 8, characterized in that, The scalp stabilizing essence also includes 0.5-3% plant extracts, which are composed of Scutellaria baicalensis root extract, Edelweiss extract and citrus peel extract in a mass ratio of 3-7:2-8:2-8.

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