Rapid hemostatic powder and preparation method thereof

Abstract

The invention provides rapid hemostatic powder and a preparation method thereof. The rapid hemostatic powder is prepared from the following components in percentage by mass: 2 to 8 percent of cannabidiol, 10 to 16 percent of polyvinyl alcohol, 7 to 12 percent of polyacrylamide, 20 to 25 percent of quaternary ammonium salt-based chitosan, 5 to 11 percent of 3-carboxyl-5-nitrophenylboronic acid, 1 to 6 percent of sodium periodate and the balance of potato starch. The hemostatic powder with a rapid hemostatic effect can be obtained by synergistically matching the components in proper mass percent. The hemostatic material has the advantages of high liquid absorption rate, high gel forming speed, short hemostatic time, good cell compatibility and the like, and is particularly suitable for the fields of accidental trauma, operations and the like. Meanwhile, the preparation method of the hemostatic material is simple and easy to implement, does not need to depend on complex processes and equipment, and is convenient for large-scale popularization.

Description

Technical Field

[0001] This invention belongs to the field of hemostatic materials technology, specifically relating to a rapid hemostatic powder and its preparation method. Background Technology

[0002] In the medical field, hemostatic materials are crucial for controlling bleeding and promoting wound healing. However, currently available hemostatic materials have many shortcomings. Traditional hemostatic materials such as gauze and bandages have low absorbency, often requiring a long time to absorb sufficient blood, and cannot quickly form an effective hemostatic barrier, resulting in prolonged hemostasis and increasing the risk of blood loss for patients. While polymeric hemostatic materials have some hemostatic effect, their gelation speed is slow, and in the face of emergency bleeding, they cannot quickly form a gel to seal the bleeding point, delaying treatment. Furthermore, some hemostatic materials have poor blood and cell compatibility, which may trigger an immune response in patients, affecting normal wound healing and even posing a potential threat to the patient's health.

[0003] Therefore, it is necessary to further improve hemostatic materials. Summary of the Invention

[0004] The purpose of this invention is to provide a hemostatic powder with rapid hemostatic effect and its preparation method.

[0005] A rapid hemostatic powder comprising the following components in weight percentages: 2-8% cannabidiol, 10-16% polyvinyl alcohol, 7-12% polyacrylamide, 20-25% quaternary ammonium chitosan, 5-11% 3-carboxy-5-nitrophenylboronic acid, 1-6% sodium periodate, and the balance being potato starch.

[0006] Optionally, the mass percentage of cannabidiol is 4-6%, the mass percentage of polyvinyl alcohol is 12-14%, the mass percentage of polyacrylamide is 9-11%, the mass percentage of quaternary ammonium chitosan is 22-24%, the mass percentage of 3-carboxy-5-nitrophenylboronic acid is 7-9%, the mass percentage of sodium periodate is 3-5%, and the balance is potato starch.

[0007] Optionally, the mass percentage of cannabidiol is 5%, the mass percentage of polyvinyl alcohol is 13%, the mass percentage of polyacrylamide is 10%, the mass percentage of quaternary ammonium chitosan is 23%, the mass percentage of 3-carboxy-5-nitrophenylboronic acid is 8%, the mass percentage of sodium periodate is 4%, and the balance is potato starch.

[0008] Optionally, the rapid hemostatic powder may also include 0.5-2% by weight of silver nanoparticles.

[0009] Optionally, the rapid hemostatic powder also includes 1-3% by weight of plant extracts, said plant extracts being one or more of aloe vera extract and witch hazel extract.

[0010] Optionally, the rapid hemostatic powder is a spherical particle with a particle size of 50-150 μm.

[0011] Optionally, the surface of the rapid hemostatic powder particles has a porous structure.

[0012] A method for preparing the above-mentioned rapid hemostatic powder includes the following steps: first, cannabidiol, 3-carboxy-5-nitrophenylboronic acid, and sodium periodate are added to anhydrous ethanol and ultrasonically dispersed evenly to obtain dispersion A; polyvinyl alcohol, polyacrylamide, and quaternary ammonium chitosan are added to deionized water and heated and stirred until dissolved to obtain solution B; under stirring conditions, dispersion A and potato starch are simultaneously added to solution B, and stirring is continued for 1-2 hours to ensure thorough mixing; then, the mixture is spray-dried, with the inlet air temperature controlled at 150-180℃ and the outlet air temperature at 80-100℃ to obtain the rapid hemostatic powder.

[0013] Optionally, polyvinyl alcohol, polyacrylamide, and quaternary ammonium chitosan are added to deionized water, heated to 25-35°C, and stirred at 100-200 rpm.

[0014] Optionally, in spray drying, the spray pressure is 0.2-0.4 MPa and the feed rate is 6-8 mL / min.

[0015] Beneficial effects In this invention, the hemostatic powder has a rapid hemostatic effect, effectively reducing bleeding time and lowering the risk of blood loss for patients. Cannabidiol, with its anti-inflammatory and cell-repair-promoting effects, can reduce wound inflammation in the hemostatic powder, creating a favorable environment for hemostasis and synergistically promoting wound healing with other components. Polyvinyl alcohol, with its excellent hydrophilicity and adhesion, can rapidly absorb water from the blood and adhere to the wound surface, helping to form a preliminary physical barrier and accelerating hemostasis in conjunction with other components. Polyacrylamide increases the viscosity of the system, promoting uniform dispersion of the components, and its network structure can capture components in the blood, synergistically accelerating the coagulation process with other components. Quaternary ammonium-based chitosan, carrying a positive charge, can interact with negatively charged blood cells, promoting platelet aggregation and activation of coagulation factors, thereby accelerating hemostasis. Its good biocompatibility, along with other components, ensures overall biocompatibility. 3-Carboxy-5-nitrophenylboronic acid can specifically bind to sugars and other substances in the blood, regulating the coagulation environment and synergistically optimizing the hemostatic process with other components. Sodium periodate can oxidize and modify some components, enhancing the interaction between them and improving the overall performance of the hemostatic powder. Potato starch, as a filler and carrier, not only ensures the uniform dispersion of other components but also participates in the coagulation process to a certain extent, working together with other components to build a highly efficient hemostatic system. Detailed Implementation

[0016] Current hemostatic materials face numerous challenges in clinical application. Regarding absorption rate, traditional hemostatic materials like gauze have limited porosity due to their fibrous structure, resulting in insufficient blood absorption. In cases of massive bleeding, they cannot quickly and effectively absorb blood, leading to continuous outflow and increased blood loss. Gel formation speed is also a significant issue; many polymeric hemostatic materials require a considerable amount of time to form a sealing gel structure after contact with blood. In emergency bleeding scenarios, this cannot promptly stop blood flow, delaying optimal treatment. Prolonged hemostasis not only causes more suffering for patients but can also lead to serious complications such as shock and organ failure due to massive blood loss, increasing the risk of death. Furthermore, hemostatic materials with poor blood and cell compatibility may trigger immune cell attacks, causing inflammatory responses, affecting wound healing, and even leading to wound infection and ulceration, thus prolonging recovery time.

[0017] Based on this, one embodiment of the present invention provides a rapid hemostatic powder, comprising the following components by mass percentage: 2-8% cannabidiol, 10-16% polyvinyl alcohol, 7-12% polyacrylamide, 20-25% quaternary ammonium chitosan, 5-11% 3-carboxy-5-nitrophenylboronic acid, 1-6% sodium periodate, and the balance potato starch. This hemostatic powder has a rapid hemostatic effect, effectively reducing bleeding time and lowering the risk of blood loss for patients. Cannabidiol has anti-inflammatory and cell-repair-promoting effects; in the hemostatic powder, it can reduce wound inflammation, creating a favorable environment for hemostasis, and synergistically promote wound healing with other components. Polyvinyl alcohol has good hydrophilicity and adhesion, rapidly absorbing water from the blood while adhering to the wound surface, helping to form a preliminary physical barrier and accelerating hemostasis in conjunction with other components. Polyacrylamide increases the viscosity of the system, promoting uniform dispersion of the components, and its own network structure can capture components in the blood, synergistically accelerating the coagulation process with other components. Quaternary ammonium-based chitosan carries a positive charge, enabling it to interact with negatively charged blood cells, promoting platelet aggregation and activation of coagulation factors, thereby accelerating hemostasis. Its excellent biocompatibility, along with other components, ensures overall biocompatibility. 3-Carboxy-5-nitrophenylboronic acid can specifically bind to substances such as sugars in the blood, regulating the coagulation environment and synergistically optimizing the hemostasis process with other components. Sodium periodate can oxidatively modify some components, enhancing the interactions between them and improving the overall performance of the hemostatic powder. Potato starch, as a filler and carrier, not only ensures uniform dispersion of other components but also participates in the coagulation process to some extent, working with other components to construct a highly efficient hemostatic system.

[0018] In some embodiments, the mass percentage of cannabidiol is 4-6%, the mass percentage of polyvinyl alcohol is 12-14%, the mass percentage of polyacrylamide is 9-11%, the mass percentage of quaternary ammonium chitosan is 22-24%, the mass percentage of 3-carboxy-5-nitrophenylboronic acid is 7-9%, the mass percentage of sodium periodate is 3-5%, and potato starch is the balance.

[0019] In some embodiments, the mass percentage of cannabidiol is 5%, the mass percentage of polyvinyl alcohol is 13%, the mass percentage of polyacrylamide is 10%, the mass percentage of quaternary ammonium chitosan is 23%, the mass percentage of 3-carboxy-5-nitrophenylboronic acid is 8%, the mass percentage of sodium periodate is 4%, and the balance is potato starch.

[0020] In some embodiments, the rapid hemostatic powder further includes 0.5-2% by weight of silver nanoparticles. The addition of silver nanoparticles utilizes their antibacterial properties to effectively inhibit bacterial growth around the wound, reducing the risk of infection.

[0021] In some embodiments, the rapid hemostatic powder further includes 1-3% by weight of plant extracts, which are one or more of aloe vera extract and witch hazel extract. The addition of plant extracts such as aloe vera extract, rich in various active ingredients, has excellent anti-inflammatory and wound-healing effects. Witch hazel extract has astringent and anti-inflammatory properties, which can further reduce wound inflammation, relieve pain, and meet more medical needs.

[0022] In this invention, aloe vera extract and witch hazel extract are water extracts of aloe vera and witch hazel, respectively.

[0023] In some embodiments, the rapid hemostatic powder is in the form of spherical particles with a particle size of 50-150 μm. Forming the hemostatic powder into spherical particles with a particle size of 50-150 μm facilitates storage, transportation, and use, and the spherical particles are more easily and evenly distributed when applied to the wound.

[0024] In some embodiments, the surface of the rapid hemostatic powder particles has a porous structure. This porous structure increases the contact area between the powder and blood, allowing for faster absorption of moisture from the blood, accelerating the gelation process, improving hemostatic efficiency, and enhancing the user experience and overall effectiveness. Another embodiment of the present invention provides a method for preparing the above-mentioned rapid hemostatic powder, comprising the following steps: first, cannabidiol, 3-carboxy-5-nitrophenylboronic acid, and sodium periodate are added to anhydrous ethanol and ultrasonically dispersed evenly to obtain dispersion A; polyvinyl alcohol, polyacrylamide, and quaternary ammonium chitosan are added to deionized water and heated and stirred until dissolved to obtain solution B; under stirring conditions, dispersion A and potato starch are simultaneously added to solution B, and stirring is continued for 1-2 hours to ensure thorough mixing; then the mixture is spray-dried, with the inlet air temperature controlled at 150-180℃ and the outlet air temperature at 80-100℃ to obtain rapid hemostatic powder.

[0025] This preparation method is simple and easy to implement, requiring no complex processes or equipment. Through steps such as ultrasonic dispersion and spray drying, the components can be uniformly dispersed, ensuring the stability and uniformity of the hemostatic powder. The spray drying process rapidly removes moisture, retains the activity of each component, and has high production efficiency, facilitating large-scale production.

[0026] In some embodiments, polyvinyl alcohol, polyacrylamide, and quaternary ammonium chitosan are added to deionized water, heated to 25-35°C, and stirred at 100-200 rpm.

[0027] In some embodiments, during spray drying, the spray pressure is 0.2-0.4 MPa and the feed rate is 6-8 mL / min.

[0028] In some embodiments, the rapid hemostatic powder is used for hemostasis in battlefield first aid, field first aid, and delicate surgeries such as oral surgery and ophthalmic surgery.

[0029] In some embodiments, the rapid hemostatic powder is sealed in an aluminum foil bag and a desiccant is placed inside the bag to improve its storage stability and extend its shelf life.

[0030] In some embodiments, the method of using the above-mentioned rapid hemostatic powder includes the following steps: Before use, clean the wound to remove surface dirt and impurities. Then, evenly sprinkle an appropriate amount of rapid hemostatic powder on the bleeding site and gently press for 1-2 minutes to ensure the powder is in full contact with the blood; finally, bandage and fix the wound with sterile gauze or a bandage.

[0031] The following are examples and comparative examples.

[0032] Example 1 In this embodiment, the preparation method of the hemostatic powder includes: first, adding cannabidiol, 3-carboxy-5-nitrophenylboronic acid, and sodium periodate to anhydrous ethanol and ultrasonically dispersing them evenly to obtain dispersion A; then, adding polyvinyl alcohol, polyacrylamide, and quaternary ammonium chitosan to deionized water, heating to 30°C, and stirring at 150 rpm until dissolved to obtain solution B; under stirring at 150 rpm, simultaneously adding dispersion A, potato starch, nano-silver particles, and plant extracts to solution B, and continuing to stir for 1.5 hours to ensure thorough mixing; then, spray drying the mixture, controlling the spray pressure at 0.3 MPa, the feed rate at 6 mL / min, the inlet air temperature at 160°C, and the outlet air temperature at 90°C to obtain a hemostatic powder with a porous surface structure. The plant extract is a mixture of aloe vera extract and witch hazel extract in a mass ratio of 1:1.

[0033] The hemostatic powders of other embodiments and comparative examples are similar to those of Example 1, with the differences shown in the table below. The numerical values ​​for the components in the table represent the mass percentage of the corresponding component in the hemostatic powder.

[0034]

[0035] Test case (1) Liquid absorption rate Using the weighing method, take a mass m1 of hemostatic powder, place it on a filter paper of known weight, put it in simulated blood, soak it for 5 minutes, take it out, place it on the filter paper for 10 minutes to filter out excess liquid, weigh it again m2, and the liquid absorption rate = (m2-m1) / m1×100%.

[0036] (2) Gel formation speed Add a certain amount of simulated blood to a petri dish, sprinkle hemostatic powder evenly on the surface of the blood, start timing, and observe and record the time from when the powder comes into contact with the blood to when a uniform gel-like substance forms.

[0037] (3) Hemostasis time A standard traumatic bleeding model was established in the legs of rats, and the rats were randomly divided into an example group, a comparative group, and a control group. The example group and the control group were treated with the hemostatic powders used in the examples and comparative examples, respectively, and the time from the application of the hemostatic powder to the complete cessation of bleeding was recorded.

[0038] (4) Cell compatibility Using the MTT assay, cultured fibroblasts were seeded into 96-well plates. After the cells adhered, hemostatic powder extract was added and cultured for 48 hours. Then, MTT reagent was added and cultured for another 4 hours. Finally, DMSO was added to dissolve the crystals. The absorbance was measured at a specific wavelength using an ELISA reader, and the cell viability was calculated. The higher the cell viability, the better the cell compatibility.

[0039]

[0040] The results above show that the hemostatic powder of the present invention has the advantages of rapid hemostasis, rapid gelation, high liquid absorption rate, and good biocompatibility.

[0041] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

[0042] For those skilled in the art, based on the ideas of this invention, there will be changes in the specific implementation methods and application scope. Therefore, the content of this specification should not be construed as a limitation of this invention.

Claims

1. A fast-acting hemostatic powder, characterized in that, The product comprises the following components by weight percentage: 2-8% cannabidiol, 10-16% polyvinyl alcohol, 7-12% polyacrylamide, 20-25% quaternary ammonium chitosan, 5-11% 3-carboxy-5-nitrophenylboronic acid, 1-6% sodium periodate, and the balance being potato starch.

2. The rapid hemostatic powder as described in claim 1, characterized in that, The mass percentages of cannabidiol are 4-6%, polyvinyl alcohol is 12-14%, polyacrylamide is 9-11%, quaternary ammonium chitosan is 22-24%, 3-carboxy-5-nitrophenylboronic acid is 7-9%, sodium periodate is 3-5%, and potato starch is the balance.

3. The rapid hemostatic powder as described in claim 1, characterized in that, The mass percentages of cannabidiol are 5%, polyvinyl alcohol is 13%, polyacrylamide is 10%, quaternary ammonium chitosan is 23%, 3-carboxy-5-nitrophenylboronic acid is 8%, sodium periodate is 4%, and potato starch is the balance.

4. The rapid hemostatic powder as described in claim 1, characterized in that, It also includes silver nanoparticles with a mass percentage of 0.5-2%.

5. The rapid hemostatic powder as described in claim 1, characterized in that, It also includes 1-3% by weight of plant extracts, which are one or more of aloe vera extract and witch hazel extract.

6. The rapid hemostatic powder according to any one of claims 1-5, characterized in that, The rapid hemostatic powder consists of spherical particles with a particle size of 50-150 μm.

7. The rapid hemostatic powder according to any one of claims 1-5, characterized in that, The surface of the rapid hemostatic powder particles has a porous structure.

8. A method for preparing a rapid hemostatic powder as described in any one of claims 1-7, characterized in that, The process includes the following steps: First, cannabidiol, 3-carboxy-5-nitrophenylboronic acid, and sodium periodate are added to anhydrous ethanol and ultrasonically dispersed to obtain dispersion A; polyvinyl alcohol, polyacrylamide, and quaternary ammonium chitosan are added to deionized water and heated and stirred until dissolved to obtain solution B; under stirring conditions, dispersion A and potato starch are simultaneously added to solution B, and stirring is continued for 1-2 hours to ensure thorough mixing; then the mixture is spray-dried, with the inlet air temperature controlled at 150-180℃ and the outlet air temperature at 80-100℃ to obtain a rapid hemostatic powder.

9. The method for preparing the rapid hemostatic powder as described in claim 8, characterized in that, Polyvinyl alcohol, polyacrylamide, and quaternary ammonium chitosan are added to deionized water, heated to 25-35℃, and stirred at 100-200 rpm.

10. The method for preparing the rapid hemostatic powder as described in claim 8 or 9, characterized in that, During spray drying, the spray pressure is 0.2-0.4 MPa and the feed rate is 6-8 mL / min.

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