Hydrogel dressing as well as preparation method and application thereof
By combining pungent glycoside, sodium alginate, and polyvinyl alcohol, a double-network gel is formed, which solves the problems of insufficient antibacterial stability and mechanical properties of existing hydrogel dressings. It achieves high water absorption, high mechanical strength, and intelligent drug release, thereby improving the quality and safety of wound care.
Patent Information
- Application Number
- CN202511651037.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-11-12
- Publication Date
- 2025-12-16
AI Technical Summary
Existing hydrogel dressings have shortcomings in antibacterial stability, mechanical strength, and intelligent drug release control, failing to meet current technological requirements and address long-term technical issues, including mechanical performance and intelligent control. These existing technological problems represent bottlenecks in the current technology, including poor antibacterial stability, insufficient mechanical properties, and unintelligent drug release mechanisms.
By using a combination of pungent glycosides, sodium alginate, and polyvinyl alcohol, physical cross-linking points are formed through freeze-thaw cycles. Combined with Ca2+ cross-linking, a double-network gel is formed, which enhances mechanical strength and antibacterial properties. Furthermore, water molecules are absorbed through hydrogen bonds formed by the high water absorption of agar seeds and the hydroxyl carboxyl groups, thus achieving intelligent drug release.
It achieves high water absorption, high mechanical strength, long-lasting antibacterial properties, and intelligent drug release that adapts to different wound environments, thus improving the quality and safety of wound care.
Smart Images
Figure CN121130159A_ABST
Abstract
Description
Technical Field
[0001] This invention belongs to the field of biomedical preparation technology, specifically relating to a hydrogel dressing, its preparation method, and its application. Background Technology
[0002] In the field of acute and chronic wound management, hydrogel wound dressings are favored due to their high water content (… With its excellent oxygen permeability and biocompatibility (90%), it has become a core material in clinical nursing. Its unique three-dimensional network structure effectively maintains a moist wound microenvironment, providing suitable conditions for granulation tissue growth and significantly accelerating epithelial cell regeneration, demonstrating irreplaceable advantages in the treatment of refractory wounds such as burns and diabetic ulcers. With the upgrading of medical needs, intelligent hydrogels integrating multiple functions such as antibacterial, anti-inflammatory, and healing-promoting properties are gradually becoming a research focus. These materials are expected to achieve precise control of infection and active regulation of the healing process by dynamically responding to changes in the wound microenvironment.
[0003] However, existing hydrogel dressings still face many technical bottlenecks in practical applications. Firstly, plant polyphenolic antibacterial components, such as pungent glycosides, have poor stability, especially at temperatures as low as -20°C. During cryopreservation, the mechanical compression caused by ice crystal formation can easily disrupt the molecular conformation, resulting in an activity retention rate of typically no more than 65% after freeze-drying, severely limiting the achievement of long-lasting antibacterial function. Secondly, the mainstream polyvinyl alcohol-sodium alginate composite gel lacks sufficient mechanical properties; its tensile strength is insufficient for the needs of moving parts such as joints, making it prone to rupture during limb movement and affecting the sustainability of wound protection. Thirdly, existing products mostly rely on a single Ca... 2+ Cross-linked systems limit drug release to passive diffusion, making it impossible to dynamically adjust the release rate based on changes in the wound microenvironment such as exudate and pH value, thus failing to meet the treatment needs of different stages of wound healing.
[0004] The aforementioned technical deficiencies result in significant shortcomings in the functional integration, environmental adaptability, and clinical applicability of existing hydrogel dressings. There is an urgent need to develop a new generation of hydrogel dressings that combine high activity retention, excellent mechanical properties, and intelligent drug release functions through material combination innovation and process optimization, in order to improve the quality of care for acute and chronic wounds. Summary of the Invention
[0005] The present invention aims to provide a hydrogel dressing, its preparation method and application. The hydrogel dressing has excellent biodegradability and safety, long-lasting antibacterial effect, high water absorption and high mechanical strength.
[0006] To solve the above-mentioned technical problems, the technical solution adopted by the present invention is as follows: A method for preparing a hydrogel dressing includes the following steps: S1. Stir the 50% ethanol solution of pomegranate glycoside powder until homogeneous to form a pomegranate glycoside-ethanol solution. S2. Dissolve sodium alginate and freeze-dried agar seeds in deionized water, degas by ultrasonication, add the pungent glycoside-ethanol solution obtained in S1, stir evenly to obtain a mixed solution. S3. Heat and stir polyvinyl alcohol and deionized water, add the mixed solution obtained in S2, stir evenly to obtain the reaction solution; S4. The reaction solution obtained in S3 is shaped in a mold, subjected to three freeze-thaw cycles, cross-linked with calcium chloride solution, freeze-dried, and sterilized to obtain a hydrogel dressing.
[0007] Preferably, in S1, the mass-to-volume ratio of the pungent glycoside powder to ethanol is 1:20.
[0008] Preferably, in S2, the mass ratio of sodium alginate to freeze-dried Lithops seeds is 3:2.
[0009] Preferably, in S2, the specific process parameters for the ultrasound are: ultrasound temperature 26°C. The ultrasonic power was 150W and the ultrasonic time was 30min.
[0010] Preferably, in step S3, the mass-to-volume ratio of polyvinyl alcohol to deionized water is 1:8, and the heating temperature is 95°C. The heating time is 3 hours.
[0011] Preferably, in S4, the specific process parameters for the freeze-thaw cycle are: freezing temperature -80°C. Freeze for 12 hours, thaw at 26°C. .
[0012] Preferably, in step S4, the specific process parameters for freeze-drying are: freezing temperature -60°C. The freezing time is 24 hours.
[0013] The present invention also provides a hydrogel dressing prepared by the preparation method described herein.
[0014] The present invention also provides the application of the hydrogel dressing as described in the preparation of wound dressing gel patches.
[0015] The present invention also provides a wound dressing gel patch, the gel patch comprising a four-layer composite structure including an adhesive layer, a backing layer, the hydrogel dressing layer, and an anti-adhesive layer.
[0016] Preferably, the adhesive layer is medical tape, the backing layer is medical non-woven fabric, and the anti-stick layer is plastic wrap.
[0017] Compared with the prior art, the present invention has the following advantages and technical effects: (1) This invention discloses a hydrogel dressing, its preparation method and application. The hydrogel dressing is made of natural and biodegradable materials. Sodium alginate and agar are natural polysaccharides that are easily decomposed and absorbed. After pungent glycoside exerts its antibacterial effect, the remaining part is easily oxidized and decomposed. The residual polyvinyl alcohol skeleton will gradually break down and be degraded by microorganisms. No toxic substances are produced in the whole process, which can effectively avoid the risk of allergies.
[0018] (2) The hydrogel dressing has long-lasting antibacterial properties. Puniculin can destroy the integrity of bacterial cell membranes, inhibit biofilm formation and interfere with energy metabolism, and has a significant inhibitory effect on Escherichia coli and Staphylococcus aureus.
[0019] (3) The hydrogel dressing has high water absorption. As a natural high-molecular hydrophilic polysaccharide, the agar seed contains a large number of hydroxyl and carboxyl groups, which can form strong hydrogen bonds with water molecules. After dissolution, the cross-linked network formed absorbs water molecules through osmotic pressure. In addition, the addition of calcium ions enhances the stability of the hydrogel structure and enables the slow release of pungent glycosides.
[0020] (4) This hydrogel dressing has high mechanical strength. Through repeated freezing and thawing, the polyvinyl alcohol molecular chains form physical cross-linking points, constructing a gradient cross-linked nanofiber reinforcement network, combined with Ca 2+ It forms a physical-chemical dual-network gel with sodium alginate carboxylate ions to create an egg-box structure, which significantly improves tensile strength and elastic modulus.
[0021] (5) It has wide practical applications and is suitable for diabetic ulcers that are prone to infection and slow to heal. It can maintain a moist microenvironment for burn wounds to promote healing and prevent surgical wound infection. For exudative wounds, the high water absorption of the seeds can lock in the exudate in time and maintain an ideal healing environment for the wound.
[0022] The technical solution of the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. Attached Figure Description
[0023] Figure 1 This is a process flow diagram of the hydrogel dressing of the present invention; Figure 2 A schematic diagram of the structure of a wound dressing gel patch; Figure 3 This image shows the antibacterial effect of a wound dressing gel patch. Detailed Implementation
[0024] The technical solution of the present invention will be further described below with reference to the accompanying drawings and embodiments.
[0025] Unless otherwise defined, the technical or scientific terms used in this invention shall have the ordinary meaning as understood by one of ordinary skill in the art to which this invention pertains.
[0026] Source of experimental materials: In this invention, unless otherwise specified, all other test materials and instruments are conventional test materials in the field and can be purchased through commercial channels.
[0027] Reference to Embodiment 1 of the present invention Figure 1 The process flow diagram is shown.
[0028] Example 1 A method for preparing a hydrogel dressing includes the following steps: S1. Dissolve 0.2g of pungent glycoside powder in 10mL of 50% ethanol and stir well to form a pungent glycoside-ethanol solution. S2. Dissolve 1.5g sodium alginate and 1g freeze-dried agar seeds in 10mL deionized water. 26 Sonicate at 150W for 30 minutes, degas, add the pungent glycoside-ethanol solution obtained from S1, stir well to obtain a mixed solution; S3. Mix 10g of polyvinyl alcohol with 80mL of deionized water. Heat and stir for 3 hours, add the mixed solution obtained from S2, stir until homogeneous, and obtain the reaction solution; S4. Set the mixture in the mold in the reaction solution obtained in S3 at -80°C. Freeze for 12 hours, 26 Thaw for 8 hours, repeat three cycles, add 100 mL of 2% calcium chloride solution for crosslinking, -60°C After freeze-drying for 24 hours and sterilization, a hydrogel dressing was obtained.
[0029] Example 2 A method for preparing a hydrogel dressing includes the following steps: S1. Dissolve 0.5g of pomegranate glycoside powder in 10mL of 50% ethanol and stir well to form a pomegranate glycoside-ethanol solution. S2. Dissolve 1.5g sodium alginate and 1g freeze-dried agar seeds in 10mL deionized water. 26 Sonicate at 150W for 30 minutes, degas, add the pungent glycoside-ethanol solution obtained from S1, stir well to obtain a mixed solution; S3. Mix 10g of polyvinyl alcohol with 80mL of deionized water. Heat and stir for 3 hours, add the mixed solution obtained from S2, stir until homogeneous, and obtain the reaction solution; S4. The reaction solution obtained in S3 is set in a mold at -80°C. Freeze for 12 hours, 26 Thaw for 8 hours, repeat three cycles, add 100 mL of 2% calcium chloride solution for crosslinking, -60°C After freeze-drying for 24 hours and sterilization, a hydrogel dressing was obtained.
[0030] Example 3 A method for preparing a hydrogel dressing includes the following steps: S1. Dissolve 0.8g of pungent glycoside powder in 10mL of 50% ethanol and stir well to form a pungent glycoside-ethanol solution. S2. Dissolve 1.5g sodium alginate and 1g freeze-dried agar seeds in 10mL deionized water. 26 Sonicate at 150W for 30 minutes, degas, add the pungent glycoside-ethanol solution obtained from S1, stir well to obtain a mixed solution; S3. Mix 10g of polyvinyl alcohol with 80mL of deionized water. Heat and stir for 3 hours, add the mixed solution obtained from S2, stir until homogeneous, and obtain the reaction solution; S4. The reaction solution obtained in S3 is set in a mold at -80°C. Freeze for 12 hours, 26 Thaw for 8 hours, repeat three cycles, add 100 mL of 2% calcium chloride solution for crosslinking, -60°C After freeze-drying for 24 hours and sterilization, a hydrogel dressing was obtained.
[0031] The effectiveness of the above embodiments was verified through the following experiments.
[0032] The hydrogel dressings provided in Examples 1-3 above are prepared into wound dressing gel patches, comprising a four-layer composite structure: an adhesive layer, a backing layer, a hydrogel dressing layer, and an anti-adhesion layer. The adhesive layer is medical tape, the backing layer is medical non-woven fabric, and the anti-adhesion layer is plastic wrap. The structure is as follows: Figure 2 As shown.
[0033] Antibacterial test: 1 sample was taken 10 5 Bacterial culture was dropped onto the surface of the hydrogel sample, covering it with a thin film, and then incubated at 37°C. The bacteria were incubated at 90% humidity for 24 hours, washed with neutralization solution, and serially diluted. Diluted solutions were plated on agar plates, and colony-forming units were counted after incubation. The results are shown in Table 1 and 2. Figure 3 As shown.
[0034] Table 1 Results of antibacterial test
[0035] From Table 1 and Figure 3It is known that the addition of pomegranate glycoside at a level of 0.3g exhibits excellent cell compatibility (cell survival rate >90%), but its antibacterial and bacteriostatic effects are limited. This formulation is suitable for clean wounds, chronic ulcers, and other conditions requiring long-term application with the goal of promoting healing, aiming to create a favorable healing microenvironment.
[0036] The dosage of pungent glycoside at 0.5g represents an optimal balance between safety and efficacy. It exhibits broad-spectrum and highly effective inhibition (>85%) against common Gram-positive bacteria (such as Staphylococcus aureus) and Gram-negative bacteria (such as Escherichia coli), while maintaining acceptable biocompatibility (cell viability >80%). This formulation has broad applicability and can be considered the first choice for treating moderately exudative wounds (such as superficial burns).
[0037] With an addition of 0.6g of pungent glycoside, significant enhancement of antibacterial properties was achieved within acceptable cytotoxicity levels (cell viability >80%), particularly with an extremely high inhibition rate against Gram-positive bacteria (>94%). This formulation is designed for infected or high-risk contaminated wounds, providing robust infection control.
[0038] When pungent glycosides were added at a concentration of 0.9g, the hydrogel exhibited extremely strong antibacterial activity (inhibition rate >96%), but also significant cytotoxicity (cell survival rate <80%). This formulation should be strictly limited to short-term, localized control of severe infections, and its powerful antibacterial efficacy must be weighed against the potential risk of inhibiting tissue repair.
[0039] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit them. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can still be made to the technical solutions of the present invention, and these modifications or equivalent substitutions cannot cause the modified technical solutions to deviate from the spirit and scope of the technical solutions of the present invention.
Claims
1. A method for preparing a hydrogel dressing, characterized in that, Includes the following steps: S1. Dissolve the pungent glycoside powder in a 50% ethanol solution and stir until homogeneous to form a pungent glycoside-ethanol solution. S2. Dissolve sodium alginate and freeze-dried agar seeds in deionized water, degas by ultrasonication, add the pungent glycoside-ethanol solution obtained in S1, stir evenly to obtain a mixed solution. S3. Heat and stir polyvinyl alcohol and deionized water, add the mixed solution obtained in S2, stir evenly to obtain the reaction solution; S4. The reaction solution obtained in S3 is shaped in a mold, subjected to three freeze-thaw cycles, cross-linked with calcium chloride solution, freeze-dried, and sterilized to obtain a hydrogel dressing.
2. The preparation method according to claim 1, characterized in that, In S1, the mass-to-volume ratio of the pungent glycoside powder to ethanol is 1:
20.
3. The preparation method according to claim 1, characterized in that, In S2, the mass ratio of sodium alginate to freeze-dried agar seeds is 3:
2.
4. The preparation method according to claim 1, characterized in that, In S2, the specific process parameters for the ultrasound are: ultrasound temperature 26°C. The ultrasonic power was 150W and the ultrasonic time was 30min.
5. The preparation method according to claim 1, characterized in that, In S3, the mass-to-volume ratio of polyvinyl alcohol to deionized water is 1:8, and the heating temperature is 95°C. The heating time is 3 hours.
6. The preparation method according to claim 1, characterized in that, In S4, the specific process parameters for the freeze-thaw cycle are: freezing temperature is -80°C. Freeze for 12 hours, thaw at 26°C. .
7. The preparation method according to claim 1, characterized in that, In S4, the specific process parameters for freeze drying are: freezing temperature -60°C. The freezing time is 24 hours.
8. The hydrogel dressing prepared by the preparation method according to any one of claims 1-7.
9. The use of the hydrogel dressing as described in claim 8 in the preparation of wound dressing gel patches.
10. A wound dressing gel patch, characterized in that, The gel patch comprises a four-layer composite structure including an adhesive layer, a backing layer, the hydrogel dressing layer as described in claim 8, and an anti-adhesive layer.