A PCR gene amplification system

Abstract

This invention discloses a PCR gene amplification system, belonging to the field of bioengineering technology. It includes: a centrifugation assembly, comprising a centrifuge disc and a first driving component for rotating the centrifuge disc; and a reaction assembly, comprising a water bath and a second driving component for adjusting the height of the water bath. The water bath has an inner ring of partitions dividing it into water bath chambers. Each water bath chamber is equipped with a temperature control device for independently controlling the water bath temperature. Each partition has a notch for centrifuge tubes to pass through and an intercepting component for closing or opening the notch. The first driving component can drive the centrifuge disc to centrifuge and switch water bath chambers. The intercepting component, in conjunction with opening and closing the notch, reduces the process of centrifuge tubes being exposed to air after leaving the water bath, preventing enzyme inactivation in the reactants within the centrifuge tubes and improving the DNA amplification success rate. Furthermore, the centrifuge tubes can quickly reach the target reaction temperature, improving the efficiency of temperature cycling, shortening the time required to reach the target amount, and increasing amplification efficiency.

Description

Technical Field

[0001] This invention relates to the field of bioengineering technology, and in particular to a PCR gene amplification system. Background Technology

[0002] Polymerase chain reaction (PCR) is a molecular biology technique used to amplify specific DNA fragments.

[0003] The principle of nucleic acid amplification is as follows: Template DNA is heated to a preset temperature, such as around 95°C, for a certain period (denaturation stage), causing the double-stranded DNA to dissociate into single strands, which then bind to primers. The temperature is then lowered to around 55°C (annealing stage), allowing the primers to pair with the complementary sequences of the template DNA single strands. Finally, the temperature is controlled at around 72°C (extension stage), where the DNA template-primer combination, under the action of Taq DNA polymerase, uses dNTPs as reactants and the target sequence as a template, synthesizing a new semi-conservative replicating strand complementary to the template DNA strand, according to the principles of base pairing and semi-conservative replication. By cycling the temperature from around 95°C to around 55°C to around 72°C, these three stages of denaturation, annealing, and extension can be repeated to obtain more "semi-conservative replicating strands." After multiple cycles, the target gene can be amplified tens of thousands or even millions of times.

[0004] Therefore, PCR gene amplification technology is essentially a temperature control technology. Existing PCR instruments use constant temperature preheating to provide a preset reaction temperature. Different water baths are used to configure and control different temperatures. When it is necessary to change the reaction temperature, the reaction tubes are moved from one water bath to another water bath at a different temperature by manual or mechanical means, thereby achieving the temperature change. However, in this process, the reaction tubes are exposed to the air medium environment, which can easily cause the enzymes in the reaction tubes to become inactive and affect the reaction results. Summary of the Invention

[0005] The purpose of this invention is to provide a PCR gene amplification system to solve one or more technical problems existing in the prior art, and at least provide a beneficial option or create conditions.

[0006] The technical solution adopted to solve the above-mentioned technical problems is as follows: A PCR gene amplification system, comprising: a centrifugation assembly, including a centrifugation tray for placing centrifuge tubes and a first driving component for rotating the centrifugation tray; a reaction assembly, including a water bath located below the centrifugation assembly and a second driving component for adjusting the height of the water bath relative to the centrifugation tray, wherein the water bath is provided with at least two partitions in the inner ring, the at least two partitions dividing the water bath into at least two water bath chambers arranged in a ring, each water bath chamber being provided with a temperature control device for independently controlling the water bath temperature, and each partition having a notch for the centrifuge tubes to pass through and an interception component for closing or opening the notch; after centrifuge tubes containing reactants are placed in the centrifugation tray for centrifugation, the height of the water bath is adjusted so that the centrifuge tubes enter one of the water bath chambers for water bath treatment for a preset time, after the water bath treatment is completed, the centrifugation tray is rotated and the notch is opened, allowing the centrifuge tubes to pass through the notch and enter the next water bath chamber with a different water bath temperature for reaction, and the notch is closed.

[0007] This technical solution has at least the following beneficial effects: After centrifugation in the centrifuge tray, the centrifuge tubes can be directly subjected to water bath treatment via an upward-moving water bath. The first driving component, in addition to rapidly rotating the centrifuge tray for centrifugation, also moves the centrifuge tubes from one water bath chamber to another. Combined with the opening and closing of the interception component, this allows the centrifuge tubes to pass through the opening into another water bath chamber, reducing the time the centrifuge tubes are exposed to air after leaving the water bath. This prevents enzyme inactivation in the reactants within the centrifuge tubes, thereby improving the DNA amplification success rate. Furthermore, the centrifuge tubes can quickly move from one water bath chamber to another at the temperature required for the next stage, enabling them to rapidly reach the target reaction temperature, improving the efficiency of temperature cycling, shortening the time required to reach the target quantity, and increasing amplification efficiency.

[0008] As a further improvement to the above technical solution, the interception assembly includes at least two interception doors rotatably mounted on the partition and a third driving member that drives each interception door to rotate independently. When the at least two interception doors rotate to close together, they form an assembly. The interception doors on the same side of the partition, when closed, jointly seal the gap. The assembly forms a closed space capable of accommodating the centrifuge tube. Driving the interception doors on the same side of the partition to rotate opens the assembly, allowing the centrifuge tube to enter and exit the closed space. Opening one interception door on one side of the partition allows the centrifuge tube to leave the current water bath chamber; closing that door allows the centrifuge tube to enter the closed space. Opening the other interception door on the other side of the partition allows the centrifuge tube to enter the next water bath chamber. Since adjacent water bath chambers cannot be directly connected, the temperature influence between adjacent water bath chambers can be reduced.

[0009] As a further improvement to the above technical solution, the assembly includes four intercepting doors, two of which are rotatably mounted on one side wall of the notch, and the other two are rotatably mounted on the other side wall of the notch. When the two intercepting doors on the same side of the partition rotate in a direction away from or towards each other, the assembly opens or closes. The simultaneous opening and closing of the two intercepting doors on the same side of the partition can accelerate the time it takes for the centrifuge tube to pass through the notch, thus accelerating the change in reaction temperature.

[0010] As a further improvement to the above technical solution, the enclosed space is adapted and fitted to the centrifuge tube to prevent indirect flow between two adjacent water bath chambers through the enclosed space.

[0011] As a further improvement to the above technical solution, the system also includes a protective component. The protective component includes a protective cover above the centrifuge disc and a fourth driving component for closing or opening the centrifuge disc with the protective cover. The first driving component includes a first drive motor mounted on top of the protective cover. The output end of the first drive motor is connected to a drive shaft extending into the protective cover. A drive ring is mounted at the bottom of the drive shaft. The centrifuge disc has a drive hole adapted to the drive ring. Simultaneously, as the fourth driving component closes or opens the centrifuge disc, the drive ring extends into the drive hole to rotate the centrifuge disc, or extends out of the drive hole to disconnect the drive connection with the centrifuge disc. When the protective cover is open, the first drive motor is disconnected from the centrifuge disc, preventing the centrifuge disc from rotating without the protection of the protective cover.

[0012] As a further improvement to the above technical solution, a first gear is coaxially mounted on the top of the transmission shaft, a second gear meshing with the first gear is mounted on the protective cover, and a first gear ring adapted to the second gear is provided on the centrifugal disc. The first gear ring is located above the transmission hole, and the centrifugal disc is provided with an idle hole that connects to the transmission hole and is located below the transmission hole. When the protective cover moves down until the transmission ring passes through the transmission hole and enters the idle hole, the second gear meshes with the first gear ring to drive the centrifugal disc to rotate.

[0013] As a further improvement to the above technical solution, a sealing edge is provided on the inner side of the protective cover, and a sealing ring is provided at the bottom of the sealing edge. When the protective cover moves down until the second gear meshes with the first gear ring, the protective cover and the water bath are mutually sealed and covered, and the sealing ring abuts against the edge of the centrifuge disc. This reduces the impact of the evaporated gas formed after the liquid in the water bath evaporates on the structure on the centrifuge disc.

[0014] As a further improvement to the above technical solution, the system further includes a support cylinder for supporting the centrifugal disc. A limiting ring is provided at the bottom of the centrifugal disc and sleeved outside the support cylinder. A track wheel assembly and a fifth driving component for driving the track wheel assembly to rise and fall are provided inside the support cylinder. The track wheel assembly includes a support frame, two drive wheels rotatably mounted on the support frame, a track sleeved on both drive wheels, and a sixth driving component for driving the track. The inner sides of the track mesh with the two drive wheels respectively. The centrifuge disc has a rack on its bottom surface that meshes with the outer side of the track. An L-shaped slider is mounted on the support frame. A groove is provided at the bottom of the centrifuge disc, allowing the L-shaped slider to slide in from one end and then be limited in position. When the fifth driving component moves the support frame upwards, the track meshes with the rack and lifts the centrifuge disc, causing the limiting ring to disengage from the support cylinder. At this time, the L-shaped slider inserts into the groove, and the track is then driven to move the centrifuge disc horizontally. The L-shaped slider slides within the groove. The track wheel assembly drives the centrifuge disc to slide horizontally, causing it to deviate from the protective cover, facilitating the entry of the pipette tip into the centrifuge tube and allowing it to be closer to the pipette, thus improving pipetting efficiency.

[0015] As a further improvement to the above technical solution, the system also includes a material turntable device, a pipetting device, and a tip-changing device. The material turntable device includes a first turntable and a seventh driving component for rotating the first turntable. The first turntable has multiple first placement holes for placing PCR tubes. The pipetting device includes a pipette and a moving component for moving the pipette in the vertical and horizontal directions. The tip-changing device includes a moving tray and an eighth driving component for moving the moving tray back and forth. The moving tray has a tip box and a sharps box arranged sequentially along the front-back direction. The first turntable, the moving tray, and the centrifuge tray are arranged side by side in the horizontal direction. After the pipette is inserted into the tip box, it aspirates the reaction mixture from the PCR tube and adds it to the centrifuge tube. The used tip is then pushed into the sharps box. This can improve the automation level of PCR gene amplification operations.

[0016] As a further improvement to the above technical solution, the system also includes a cap-opening and closing assembly. The centrifuge tube and / or the PCR tube are reaction tubes with caps. One end of the cap is flexibly connected to the reaction tube, and the other end has a protrusion extending out of the reaction tube. The cap-opening and closing assembly includes an extension plate and a ninth driving component for rotating the extension plate. The extension plate has a transverse groove for the protrusion to enter horizontally, and an airflow channel. One end of the airflow channel is connected to a positive and negative gas source, and the other end forms a suction cup opening corresponding to the cap. When the protrusion of the cap is in the transverse groove, the positive and negative gas sources provide negative pressure, causing the cap to be adsorbed at the suction cup opening. Starting the extension plate rotates the cap, which can rotate the cap to remove or insert it into the reaction tube. This allows for quick and accurate opening and insertion of the cap. Attached Figure Description

[0017] The above and / or additional aspects and advantages of the present invention will become apparent and readily understood from the description of the embodiments taken in conjunction with the following drawings, in which:

[0018] Figure 1 This is a schematic diagram of the overall structure of an embodiment of the present invention;

[0019] Figure 2 This is a top view of the extension plate in an embodiment of the present invention;

[0020] Figure 3 This is a perspective view of the extension plate in an embodiment of the present invention;

[0021] Figure 4 This is a schematic diagram of the bottom of the extension plate in an embodiment of the present invention;

[0022] Figure 5 This is a schematic diagram of the extension plate opening the plug in an embodiment of the present invention;

[0023] Figure 6 This is a top view of the structure according to an embodiment of the present invention;

[0024] Figure 7 for Figure 6 Schematic diagram of the cross section of AA;

[0025] Figure 8 This is a schematic diagram of the structure when the centrifuge disc is moved horizontally in an embodiment of the present invention;

[0026] Figure 9 This is a schematic diagram of the structure when the centrifuge disc is not moved horizontally in an embodiment of the present invention;

[0027] Figure 10 This is a cross-sectional schematic diagram of the track wheel assembly lifting the centrifugal disc in an embodiment of the present invention (in a state where the centrifugal disc can be moved).

[0028] Figure 11 This is a cross-sectional schematic diagram of the track wheel assembly detached from the centrifugal disc and the transmission ring inserted into the conventional hole in an embodiment of the present invention (centrifugally processable state);

[0029] Figure 12 This is a cross-sectional schematic diagram of the second gear meshing with the first gear ring and the water bath being moved upwards (in a water bath treatment state) in an embodiment of the present invention.

[0030] Figure 13 This is a schematic diagram of the top of the centrifuge tray in an embodiment of the present invention;

[0031] Figure 14 This is a schematic diagram of the bottom of the centrifuge tray in an embodiment of the present invention;

[0032] Figure 15 This is a schematic diagram of the top of the water bath tank in an embodiment of the present invention;

[0033] Figure 16 This is a schematic diagram of the track wheel assembly in an embodiment of the present invention;

[0034] Figure 17 This is a schematic diagram of the interception component, partition, and temperature control in an embodiment of the present invention;

[0035] Figure 18 This is a schematic diagram showing the distribution of multiple partitions and interception components in an embodiment of the present invention;

[0036] Figure 19 This is a schematic diagram of the process of centrifuge tubes passing through the partition when two intercepting doors are provided on one side of the partition in an embodiment of the present invention;

[0037] Figure 20 This is a schematic diagram of the process of centrifuge tubes passing through a partition when an intercepting door is provided on one side of the partition in an embodiment of the present invention.

[0038] 100. Workbench; 110. PCR tube; 120. Centrifuge tube; 130. Cap; 131. Protrusion; 140. Connecting strip;

[0039] 200. Material turntable device; 210. First turntable; 211. First placement hole; 220. Seventh driving component;

[0040] 300. Pipettes; 310. Pipettes; 320. Moving parts;

[0041] 400. Head changing device; 410. Moving tray; 420. Eighth drive unit; 430. Suction head box; 440. Sharps box;

[0042] 500, Centrifuge assembly; 510, Centrifuge disc; 511, Transmission hole; 512, Idle hole; 520, First driving component; 521, First drive motor; 522, Transmission shaft; 523, Transmission ring; 524, First gear; 525, Second gear; 526, First gear ring;

[0043] 600. Protective component; 610. Protective cover; 620. Sealing edge; 621. Sealing ring; 630. Fourth drive component;

[0044] 700. Reaction assembly; 710. Water bath; 720. Partition; 721. Notch; 730. Water bath chamber; 740. Interception assembly; 741. Interception door; 742. Third drive unit; 760. Temperature control unit; 761. Partition mesh; 762. Electric heating rod; 763. Water pipe; 764. Temperature sensor; 770. Second drive unit;

[0045] 800, Support cylinder; 810, Limiting ring; 820, Track wheel assembly; 821, Support frame; 822, Drive wheel; 823, Track; 824, Sixth drive component; 830, Rack; 840, L-shaped slider; 850, Slide groove; 860, Fifth drive component;

[0046] 900, Opening / closing cover assembly; 910, Extension plate; 920, Horizontal groove; 930, Airflow channel; 940, Suction cup port; 950, Ninth drive component. Detailed Implementation

[0047] Embodiments of the present invention are described in detail below. Examples of these embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and should not be construed as limiting the present invention.

[0048] In the description of this invention, it should be understood that the orientation descriptions, such as up, down, front, back, left, right, etc., are based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limiting this invention.

[0049] In the description of this invention, "several" means one or more, "more than" means two or more, "greater than," "less than," and "exceeding" are understood to exclude the stated number, while "above," "below," and "within" are understood to include the stated number. The use of "first" and "second" in the description is merely for distinguishing technical features and should not be construed as indicating or implying relative importance, or implicitly indicating the number of indicated technical features, or implicitly indicating the order of the indicated technical features.

[0050] In the description of this invention, unless otherwise explicitly defined, terms such as "set up," "install," and "connect" should be interpreted broadly, and those skilled in the art can reasonably determine the specific meaning of the above terms in this invention in conjunction with the specific content of the technical solution.

[0051] Reference Figure 1-20 A PCR gene amplification system includes a workbench 100, a material turntable 200, a pipetting device 300, a head-changing device 400, a centrifuge assembly 500, a protective assembly 600, a reaction assembly 700, a support tube 800, and a lid-opening / closing assembly 900. The material turntable 200, pipetting device 300, head-changing device 400, centrifuge assembly 500, protective assembly 600, reaction assembly 700, support tube 800, and lid-opening / closing assembly 900 are all mounted on the workbench 100. The reaction materials used for gene amplification can be pre-loaded into PCR tubes 110. Centrifuge tubes 120 can be used as containers for mixing various reaction materials. Both the PCR tube and the centrifuge tube 120 are reaction tubes with a conical bottom. A flexible connecting strip 140 is provided at the top of the reaction tube. A cap 130 for sealing the top opening of the reaction tube is connected to the end of the connecting strip 140 away from the reaction tube. A protrusion 131 protruding from the outer wall of the reaction tube is provided on the side of the cap 130 away from the connecting strip 140, so that the cap 130 can be opened by applying force to the protrusion 131.

[0052] Specifically, refer to Figure 1 The material turntable device 200 includes a first turntable 210 and a seventh drive member 220. The first turntable 210 is rotatably disposed relative to the worktable 100, and the seventh drive member 220 is mounted on the worktable 100. The first turntable 210 has multiple first placement holes 211 around its rotation center at its edge, into which PCR tubes 110 can be inserted and secured. In other embodiments, to improve the stability of the PCR tubes 110 within the first placement holes 211, a clamping hand for holding the PCR tubes can also be provided on the first turntable 210 to enhance the stability of the PCR tubes and provide a force basis for the subsequent automatic opening of the cap 130 of the PCR tubes 110 by the cap opening and closing assembly 900.

[0053] The seventh driving component 220 can drive the first turntable 210 to rotate. For example, the seventh driving component 220 can be a first motor. The first motor is installed on the worktable 100 with its output end facing upward. The output end of the first motor is connected to the bottom center of the first turntable 210, so that the first motor can drive the first turntable 210 to rotate.

[0054] The pipetting device 300 includes a pipette 310 and a moving assembly 320. The pipette 310 is an electrically controlled pipetting device with a controller, which can perform operations such as aspiration, dispensing, and tip discarding at specified times by setting parameters. The specific structure of the pipette 310 is prior art and will not be described in detail here. In other embodiments, the pipette 310 may also consist of a manual pipette 310 and a telescopic drive / or a rotary drive. The telescopic drive / or rotary drive operates the button in the manual pipette 310, thereby realizing operations such as aspiration, dispensing, and tip discarding. The moving assembly 320 can drive the pipette 310 to move in the vertical and left-right directions. The moving assembly 320 includes a first moving block that slides left and right and a second moving block that slides vertically on the first moving block. The second moving block is provided with a gripper for holding the pipette 310. The left-right sliding of the first moving block and the vertical sliding of the second moving block can be driven by motors and screws respectively, or an electric slide rail can be used. In other embodiments, the moving component 320 may also be implemented using a robotic arm.

[0055] The pipette tip changing device 400 includes a moving tray 410, an eighth driving component 420, a tip holder 430, and a sharps holder 440. The moving tray 410 is slidably mounted on the worktable 100 in a front-to-back direction. The tip holder 430 and sharps holder 440 are fixed to the top of the moving tray 410 at opposite positions. Multiple pipette tips are arranged in a matrix on the tip holder 430; these tips are disposable tapered suction tubes inserted into the end of a pipette 310. The sharps holder 440 is used to hold used pipette tips for subsequent standardized processing. The tip holder 430 and sharps holder 440 are spaced a certain distance apart.

[0056] The eighth drive unit 420 can drive the movable tray 410 to slide in the front-to-back direction, thereby switching between the tip box 430 and the sharps box 440 to align with the left-to-right movement path of the pipette 310. The eighth drive unit 420 includes an eighth motor and a threaded rod mounted on the output end of the eighth motor, the threaded rod being threadedly connected to the movable tray 410. The eighth motor drives the threaded rod to rotate, thereby driving the movable tray 410 to slide back and forth. In other embodiments, the eighth drive unit 420 may also be a cylinder, a hydraulic cylinder, or an electric telescopic cylinder.

[0057] When a new pipette tip needs to be inserted into pipette 310, the eighth drive unit 420 moves the tip holder 430 to a position corresponding to the left-right movement path of pipette 310. The moving component 320 then moves pipette 310 above the tip to be inserted, allowing the new tip to be inserted during the downward movement of pipette 310. When a used pipette tip needs to be discarded, the eighth drive unit 420 moves the sharps holder 440 to a position corresponding to the left-right movement path of pipette 310. The moving component 320 then moves pipette 310 above the opening of the sharps holder 440, causing pipette 310 to eject the used tip, which falls into the sharps holder 440 for safekeeping and proper disposal.

[0058] Reference Figure 1-5 There are two opening and closing cap assemblies 900. The first opening and closing cap assembly 900 is mounted on the top of the workbench 100 for opening or closing the cap 130 of the PCR tube 110. The opening and closing cap assembly 900 includes an extension plate 910 and a ninth drive unit 950.

[0059] The ninth driving component 950 is a ninth motor mounted on the workbench 100. One end of the extension plate 910 has a rotating shaft structure. The output end of the ninth motor is connected to the rotating shaft structure of the extension plate 910, or the output end of the ninth motor is connected to the rotating shaft structure of the extension plate 910 via a reducer, thereby enabling the ninth motor to drive the extension plate 910 to rotate. Both the ninth motor and the rotating shaft of the extension plate 910 are located outside the first turntable 210 to prevent interference between the ninth motor and the extension plate 910 and other PCR tubes 110. A transverse groove 920 is provided at the end of the extension plate 910 away from the rotating shaft structure, making this end of the extension plate 910 C-shaped. The opening of the transverse groove 920 faces the center of the first turntable 210.

[0060] As the first turntable 210 rotates, the protrusions 131 on the caps 130 of the PCR tubes 110 mounted on the first turntable 210 sequentially pass through the transverse grooves 920. When it is necessary to open one of the PCR tubes 110, the first turntable 210 is rotated to the position of the extension plate 910 corresponding to that PCR tube 110 near the transverse groove 920, so that the protrusions 131 on the caps 130 of that PCR tube are embedded in the transverse groove 920. Then, the ninth drive unit 950 drives the extension plate 910 to rotate, thereby rotating the caps 130 of the PCR tube and opening the caps 130. After the liquid is collected, the ninth drive unit 950 is driven in the opposite direction to drive the extension plate 910 to rotate in the opposite direction, thereby rotating the caps 130 of the PCR tube in the opposite direction and closing the caps 130 in time to prevent contamination of the PCR tube reaction.

[0061] Understandably, since the pivot of the extension plate 910 is located outside the first turntable 210, the rotation center line of the extension plate 910 can be positioned within the area enclosed by the connecting strip 140. This allows the extension plate 910 to adapt to the deformation of the connecting strip 140 during the opening and closing process of the stopper 130, ensuring that the position of the stopper 130 is not easily affected by the deformation of the connecting strip 140. The extension plate 910 will also not interfere with the connecting strip 140 or other parts of the PCR tube 110. Furthermore, controlling the rotation center line of the stopper 130 within the area enclosed by the connecting strip 140 improves the accuracy and stability of opening and closing the stopper 130.

[0062] Furthermore, the extension plate 910 has an internal airflow channel 930, the length of which is parallel to the length of the extension plate 910. One end of the airflow channel 930 extends from the end of the extension plate 910 near the ninth drive member 950 and connects to a positive and negative gas source, which can provide compressed gas and negative pressure gas to the airflow channel 930. The other end of the airflow channel 930 extends from the position near the transverse groove 920 and forms a suction cup opening 940. The size of the suction cup opening 940 is smaller than the size of the top surface of the plug cover 130, so that the top surface of the plug cover 130 can completely cover the suction cup opening 940.

[0063] When the protrusion 131 extends into the transverse groove 920, the suction cup opening 940 is directly opposite the top surface of the cap 130. The positive and negative gas sources provide negative pressure gas, ensuring the cap 130 is firmly attached to the suction cup opening 940, thus fixing the position of the cap 130 relative to the extension plate 910 and preventing the cap 130 from falling out of the extension plate 910's range. During the process of rotating the cap 130 to open or close it, the positive and negative gas sources continuously provide negative pressure gas to ensure the cap 130 does not detach from the control of the extension plate 910. After the cap 130 is closed, the positive and negative gas sources provide compressed gas, allowing the cap 130 to easily detach from the suction cup opening 940 and to move out of the control range of the extension plate 910 during the rotation of the first turntable 210. When the positive and negative gas sources provide compressed gas, the compressed gas pushes the cap 130 tightly into the PCR tube, ensuring a stable cap 130. A sealing ring can be attached to the edge of the extension plate 910 at the suction cup opening 940 to improve the sealing between the edge of the suction cup opening 940 and the top surface of the plug 130, and to ensure the stability of the connection between the extension plate 910 and the plug 130.

[0064] In other embodiments, the first opening and closing cap assembly 900 may not be provided. Instead, the PCR tube 110 can be manually opened by opening the cap 130 before being placed on the first turntable 210.

[0065] Reference Figure 1 and Figure 7The protective component 600, the centrifugal component 500, and the reaction component 700 are arranged sequentially from top to bottom on the workbench 100.

[0066] Reference Figure 10-12 The protective assembly 600 includes a protective cover 610 and a fourth driving member 630. The fourth driving member 630 can drive the protective cover 610 to move vertically. The fourth driving member 630 includes a fourth motor and a screw rod. A support frame is mounted on the top of the protective cover 610, and a guide rod passes through the support frame. A top plate is mounted on the top of the worktable 100. The guide rod is vertically mounted at the bottom of the top plate. The fourth motor is mounted on the top of the top plate, and its output end extends downwards through the top plate and connects to the screw rod. The fourth motor can drive the screw rod to rotate, thereby causing the support frame and the protective cover 610 to move up and down together, realizing the opening and closing process of the protective cover 610. In other embodiments, the fourth driving member 630 can also be implemented using a cylinder, a hydraulic cylinder, or an electric telescopic rod.

[0067] Reference Figure 10-14 The centrifuge assembly 500 includes a centrifuge disc 510 and a first drive component 520. A support cylinder 800 is mounted on the top of the workbench 100, and a limit ring 810 is mounted on the bottom of the centrifuge disc 510. The limit ring 810 is adapted to fit on the top of the support cylinder 800, allowing the centrifuge disc 510 to rotate stably relative to the workbench 100. A second placement hole is provided on the edge of the centrifuge disc 510 for inserting and securing centrifuge tubes 120. The centrifuge disc 510 is positioned below the protective cover 610, so that when the protective cover 610 moves down, it can cover the outside of the centrifuge disc 510, thereby achieving a protective function. To improve the stability of the centrifuge tubes 120 on the centrifuge disc 510, a clamping hand or other fixing component for holding the centrifuge tubes 120 can be installed on the centrifuge disc 510.

[0068] The first driving component 520 includes a first driving motor 521, a transmission shaft 522, and a transmission ring 523. The first driving motor 521 is mounted on top of the protective cover 610. The output end of the first driving motor 521 passes through the protective cover 610 from top to bottom and is connected to the transmission shaft 522. The transmission shaft 522 is rotatably connected to the protective cover 610 via bearings. The transmission ring 523 is mounted on the bottom of the transmission shaft 522. An extension cylinder is coaxially arranged on the top of the centrifugal disc 510. A transmission hole 511 is opened on the top of the extension cylinder. The transmission hole 511 is adapted to the transmission ring 523, meaning that the cross-section of the internal space of the transmission hole 511 is the same as the cross-section of the outer edge of the transmission ring 523 and is non-circular, allowing the transmission ring 523 to be connected to the centrifugal disc 510 via transmission. When the fourth driving component 630 moves the protective cover 610 downward, it simultaneously drives the transmission ring 523 to insert into the transmission hole 511, making the transmission ring 523 connected to the centrifuge disc 510. Then, it drives the first driving motor 521, causing the centrifuge disc 510 to rotate and centrifuge the solution in the centrifuge tube 120. When the fourth driving component 630 moves the protective cover 610 upward, it simultaneously drives the transmission ring 523 to extend out of the transmission hole 511, disconnecting the transmission connection between the transmission ring 523 and the centrifuge disc 510.

[0069] The first turntable 210, the moving tray 410, and the centrifuge tray 510 are arranged side-by-side in a left-right direction. Specifically, the first placement hole 211 corresponding to the suction cup opening 940 on the extension plate 910 in the first opening and closing cap assembly 900, the moving tray 410, and the position of the centrifuge tube 120 when the centrifuge tray 510 is rotated to the preset position are all on the same vertical plane. Thus, by simply moving the pipette 310 with the moving component 320 in the vertical and left-right directions, a series of cyclical operations can be completed, including attaching the pipette tip to the tip holder 430, drawing the reaction material from the open PCR tube 110, transferring the drawn reaction material to the centrifuge tube 120, and discarding the used pipette tip into the sharps holder 440. The coordinated operation between different devices and components can shorten the overall solution preparation time and improve the efficiency of gene amplification.

[0070] Reference Figure 7-11 and Figure 15-16 To facilitate the movement of the pipette 310 above the centrifuge tube 120 for pipetting and to reduce the required height of the protective cover 610, a track wheel assembly 820 and a fifth drive component 860 are installed inside the support cylinder 800. The track wheel assembly 820 includes a support frame 821, two drive wheels 822, a track 823, and a sixth drive component 824. The fifth drive component 860 is a fifth telescopic cylinder installed inside the support cylinder 800 with its output end vertically upward. The fifth telescopic cylinder can be a pneumatic cylinder, a hydraulic cylinder, or an electric telescopic cylinder.

[0071] A support frame 821 is mounted on the output end of the fifth telescopic cylinder, allowing the fifth telescopic cylinder to drive the support frame 821 to move up and down. Two drive wheels 822 are rotatably mounted on the support frame 821, and a track 823 is simultaneously fitted around the outer periphery of the two drive wheels 822, so that when one drive wheel 822 rotates, it drives the track 823, and when the track 823 rotates, it also drives the other drive wheel 822 to rotate. A sixth drive unit 824 is used to drive the track 823. The sixth drive unit 824 is a sixth motor mounted on the support frame 821. A third gear is mounted on one side of each of the two drive wheels 822, and a fourth gear is mounted on the output end of the sixth motor. Both third gears mesh with the fourth gear, and the two third gears have the same number of teeth, ensuring that the rotation direction and speed of the two third gears are the same. When the sixth motor drives the fourth gear to rotate, it synchronously drives the two third gears and the two drive wheels 822 to rotate, thereby ensuring the stable operation of the track 823. In other embodiments, the sixth motor can also directly drive one of the drive wheels 822 to rotate.

[0072] The track 823 has teeth on both its inner and outer sides, allowing the outer circumferences of the two drive wheels 822 to mesh with the inner side of the track 823 for transmission, improving transmission stability and accuracy. A rack 830 is located in the center of the bottom of the centrifuge disc 510, extending away from the centrifuge tube 120. The rack 830 is matched with the teeth on the outer side of the track 823.

[0073] L-shaped sliders 840 are provided on both sides of the support frame 821. The tops of the two L-shaped sliders 840 are bent in opposite directions to form an L shape. Slots 850 are provided on both sides of the bottom of the centrifuge disc 510, near the rack 830. One end of the slot 850 is within the range of the limiting ring 810, and the other end extends away from the centrifuge tube 120. That is, the slot 850 and the rack 830 are arranged parallel to each other, and the length direction of both the slot 850 and the rack 830 is left-right. The end of the slot 850 within the limiting ring 810 has a port for the corresponding L-shaped slider 840 to extend vertically into. The opening size of the port of the slot 850 is larger than the opening size at other locations of the slot 850. It should be noted that a clearance opening with a clearance rack 830 and two sliding grooves 850 is formed on one side of the limiting ring 810. The width of the clearance opening is smaller than the diameter of the limiting ring 810 to ensure the relative rotation restriction between the limiting ring 810 and the support cylinder 800.

[0074] When the centrifuge disc 510 needs to be moved horizontally left or right, first rotate the centrifuge disc 510 until the centrifuge tube 120 is positioned on the left side closest to the moving tray 410. Drive the fifth drive component 860 to move the track wheel assembly 820 upward as a whole, so that the two L-shaped sliders extend into the corresponding grooves 850. At the same time, the outer side of the track 823 meshes with the rack 830. Continue to drive the fifth drive component 860 to move the track wheel assembly 820 upward as a whole, so as to lift the centrifuge disc 510, so that the limiting ring 810 is released from the relative restriction of the support cylinder 800, allowing the centrifuge disc 510 to move horizontally. In order to ensure the stability of the centrifuge disc 510 at this time, a magnetic component that can attract each other can be installed between the outer side of the track 823 and the rack 830. The magnetic component provides a suitable force so that the centrifuge disc 510 is not easy to shake on the track 823, thereby ensuring the stability of the centrifuge disc 510. The sixth drive component 824 is then activated, causing the track 823 to operate, which in turn drives the centrifuge disc 510 to move to the left. After the centrifuge disc 510 moves to the left a certain position, the L-shaped slider 840 slides within the groove 850 and moves away from the port position, ensuring that the centrifuge disc 510 does not detach from the support frame 821. That is, the centrifuge disc 510 can only slide left and right relative to the support frame 821. After moving the centrifuge disc 510 to the left a preset distance, the centrifuge tube 120 on the centrifuge disc 510 can extend beyond the coverage area of ​​the protective cover 610, thereby facilitating the movement of the pipette 310 above the centrifuge tube 120 for smooth and efficient preparation of the reaction solution. After the reaction solution is prepared, the centrifuge disc 510 is driven to move to the right until the limiting ring 810 corresponds to the support cylinder 800. The fifth driving component 860 is then driven to move the track wheel assembly 820 downward, so that the L-shaped slider extends out of the slide groove 850 and enters the support cylinder 800. The track 823 disengages from the rack 830, and the relative rotation restriction between the centrifuge disc 510 and the support cylinder 800 is re-established, ensuring the stability of the centrifuge disc 510 during centrifugal rotation.

[0075] Reference Figure 1-5The second capping assembly 900 is mounted on top of the workbench 100 and is used to open or close the cap 130 of the centrifuge tube 120. The first and second capping assemblies 900 have the same structure, but may differ in size. The extension plate 910 in the second capping assembly 900 is positioned close to the centrifuge tray 510, with the opening of the transverse groove 920 of the extension plate 910 facing the center of the centrifuge tray 510, but the extension plate 910 is not within the coverage area of ​​the protective cover 610. When the centrifuge tray 510 moves to the left, it causes the centrifuge tube 120 to extend out of the protective cover 610, and the protrusion 131 of the cap 130 of the centrifuge tube 120 just extends into the transverse groove 920 of the capping extension plate 910. Then, the ninth drive unit 950 is driven, which enables the timely opening and closing of the cap of the centrifuge tube 120. The specific principle can be referred to the action of the first capping assembly 900 in opening and closing the cap 130 of the PCR tube 110, which will not be repeated here. In other embodiments, the second capping assembly 900 may be omitted. Instead, the cap 130 of the centrifuge tube 120 may be manually opened before being placed on the centrifuge tray 510. After pipetting, the cap 130 of the centrifuge tube 120 may be manually closed.

[0076] Reference Figure 10-13 , Figure 15 as well as Figure 17-19 The reaction assembly 700 includes a water bath 710, a second driving component 770, and at least two partition plates 720. A sliding cylinder, fitted around a support cylinder 800, is located in the middle of the water bath 710. The sliding cylinder can slide up and down relative to the support cylinder 800, thus guiding the vertical movement of the water bath 710. The second driving component 770 is a second telescopic cylinder, which can be a pneumatic cylinder, hydraulic cylinder, or electric telescopic cylinder. The second telescopic cylinder is installed inside the support cylinder 800 with its output end facing downwards. A mounting plate is installed at the output end of the second telescopic cylinder, with both ends of the mounting plate extending out of the support cylinder 800 and connecting to the bottom of the water bath 710. Both sides of the support cylinder 800 have clearance grooves for the mounting plate to slide. Driving the output end of the second telescopic cylinder to retract moves the water bath 710 upwards; driving the output end of the second telescopic cylinder to extend moves the water bath 710 downwards, thereby adjusting the height of the water bath 710.

[0077] The water bath 710 is located below the centrifuge disc 510. In this embodiment, six partitions 720 are provided, and the six partitions 720 are evenly arranged around the water bath 710, so that the internal space of the water bath 710 is evenly divided into six fan-shaped water bath chambers 730.

[0078] In other embodiments, the partitions 720 may be two, three, four, or five, and correspondingly, the water bath 710 is divided by the partitions 720 to form two, three, four, or five water bath chambers 730. Each water bath chamber 730 is equipped with an independent temperature control device 760. The temperature control device 760 includes a mesh 761, an electric heating rod 762, a water pipe 763, and a temperature sensor 764. The water pipe 763 is connected to the water tank through a valve, which can provide low-temperature water to the water bath chamber 730, and can also be used to remove liquid from the water bath chamber 730. The electric heating rod 762 can heat the liquid in the water bath chamber 730 when energized, and the heating efficiency of the electric heating rod 762 can be controlled by controlling the power, thereby controlling the water temperature. A mesh 761 covers the lower middle part of the water bath chamber 730. The electric heating rod 762 and water pipe 763 are both located below the mesh 761. The temperature sensor 764 is located at the top center of the mesh 761. When the centrifuge tube 120 enters the water bath chamber 730 for water bath treatment, the bottom of the centrifuge tube 120 is a certain distance above the mesh 761. The temperature sensor 764 is used to detect the temperature of the liquid inside the water bath chamber 730. Based on the temperature of the temperature sensor 764 and the current temperature, the electric heating rod 762 can be controlled to heat the liquid, or cooling water can be introduced through a valve on the water pipe 763 to cool it, so that the water bath chamber 730 can quickly reach and maintain the target temperature.

[0079] Each partition 720 has a notch 721 at its top and an interceptor assembly 740. The outline of the notch 721 is adapted to the outer outline of the centrifuge tube 120. The interceptor assembly 740 includes at least two interceptor doors 741 and a third drive member 742. In this embodiment, one interceptor assembly 740 includes four interceptor doors 741 and a third drive member 742. The four interceptor doors 741 are rotatably connected to the partition 720. The rotatable connection can be achieved through flexible sheets or flexible strips, or through hinges. The third drive member 742 can individually drive each interceptor door 741 to rotate relative to the partition 720. The third drive member 742 includes the same number of third telescopic cylinders as the number of interceptor doors 741. The third telescopic cylinder can be a pneumatic cylinder, a hydraulic cylinder, or an electric telescopic cylinder. The third telescopic cylinder is rotatably mounted on the partition 720, and its output end is rotatably mounted on the interceptor door 741. Through the telescopic movement of the third telescopic cylinder, the interceptor door 741 can be driven to rotate. In other embodiments, the third drive unit 742 may also include the same number of third motors as the intercepting gate 741. The third motor is mounted on the partition 720, and the output end of the third motor is connected to the rotation center of the intercepting gate 741. By driving the third motor, the intercepting gate 741 can be rotated.

[0080] Specifically, the four intercepting doors 741 can be rotated to overlap each other to form an assembly. The interior of the assembly forms a closed space that fits and conforms to the outer side of the centrifuge tube 120 to accommodate it. The sides of two adjacent intercepting doors 741 that are close to each other are rotatably mounted on one side wall of the corresponding notch 721, and the sides of two other adjacent intercepting doors 741 that are close to each other are rotatably mounted on the other side wall of the corresponding notch 721.

[0081] The two intercepting doors 741 located on the same side of the partition 720 can open and move away from each other during rotation, allowing the centrifuge tube 120 in the water bath chamber 730 on that side to enter the space enclosed by the intercepting doors 741, thus enabling the centrifuge tube 120 to enter the enclosed space of the assembled body. The two intercepting doors 741 located on the same side of the partition 720 can also close and move away from each other during rotation, forming the assembled body and creating a closed space for the centrifuge tube 120 to be accommodated.

[0082] It is understandable that when the two intercepting doors 741 located on the same side of the partition 720 are closed, they can seal the gap 721 to separate the two adjacent water bath chambers 730. After centrifugation, the second driving component 770 is driven to adjust the height of the water bath 710, so that the centrifuge tube 120 enters the water bath chamber 730 with the water bath temperature pre-adjusted. When the first stage of water bath is completed, the intercepting door 741 on the side of the partition 720 in the current water bath chamber 730 is initially open or is open at this time. When the first driving component 520 drives the centrifuge disc 510 to rotate, it drives the centrifuge tube 120 into the range of the intercepting door 741 and closes the intercepting door 741 on the side of the partition 720 in the current water bath chamber 730. The intercepting door 741 on the side of the partition 720 in the next water bath chamber 730 is opened, and the centrifuge tube 120 continues to move and enters the water bath chamber 730 that has reached the target temperature in advance to carry out the next stage of reaction. This cycle is used to achieve efficient gene amplification. In addition, the number of water bath chambers 730 can be equal to the number of temperature changes experienced in the cycle process or a multiple thereof. Then, each water bath chamber 730 only needs to maintain the target temperature of the corresponding stage in the subsequent cycle process.

[0083] Centrifuge tube 120 can be quickly moved from one water bath chamber 730 to another, allowing the centrifuge tube to rapidly reach the target reaction temperature, improving the efficiency of temperature cycling, shortening the time required to reach the target volume, and increasing amplification efficiency. Furthermore, when centrifuge tube 120 crosses between two adjacent water bath chambers 730, it does not leave the water bath solution and come into contact with the air medium, ensuring stable and controllable temperature changes. This prevents enzyme inactivation in the reactants within the centrifuge tube and improves the success rate of DNA amplification.

[0084] Reference Figure 20In other embodiments, an interception assembly 740 may also include two interception gates 741 with arc-shaped cross-sections. The two interception gates 741 are rotatably mounted on the two side walls of the notch 721, respectively, on opposite sides. The assembly formed after closing the two interception gates 741 also forms a closed space that perfectly fits the outer wall of the centrifuge tube 120. The process of the centrifuge tube 120 passing through this interception assembly 740 can be referred to the centrifuge tube 120 passage operation process of the interception assembly 740 composed of four interception gates described above, and will not be repeated here.

[0085] Furthermore, referring to Figure 10-12 A first gear 524 is coaxially mounted on the top of the drive shaft 522, and a second gear 525 is rotatably mounted on the inner bottom of the protective cover 610. The second gear 525 meshes with the first gear 524. A first gear ring 526 is provided on the top of the centrifugal disc 510. The diameter of the first gear ring 526 is larger than the diameter of the drive hole 511, and the first gear ring 526 is located above the drive hole 511. An idle hole 512 is provided at the bottom of the extension cylinder of the centrifugal disc 510, and the idle hole 512 is located below the drive hole 511.

[0086] When preparing for water bath treatment, as the water bath 710 rises, the fourth drive component 630 can cause the protective cover 610 to continue moving downwards, allowing the transmission ring 523 to pass through the transmission hole 511 and enter the idling hole 512. This prevents the transmission ring 523 from driving the centrifuge disc 510 to rotate. Simultaneously, the second gear 525 meshes with the first gear ring 526, and the first drive motor 521 drives the centrifuge disc 510 to rotate via the first gear 524, the second gear 525, and the first gear ring 526. This achieves speed reduction and torque increase, allowing the first drive motor to provide greater power for moving the centrifuge tube 120 within the water bath solution.

[0087] The inner side of the protective cover 610 is provided with a sealing edge 620, and a sealing ring 621 is attached to the bottom of the sealing edge 620. When preparing for water bath treatment, during the upward process of the water bath tank 710, the fourth driving component 630 drives the protective cover 610 to continue to move downward until the second gear 525 and the first gear ring 526 are engaged. That is, the centrifugal disc 510 is rotated by the first gear 524, the second gear 525 and the first gear ring 526, and then the protective cover stops. At this time, the sealing ring 621 abuts against the edge of the centrifugal disc 510, that is, the sealing ring 621 is clamped between the sealing edge 620 and the centrifugal disc 510. The bottom of the protective cover 610 is closed and fitted onto the outside of the water bath tank 710, thereby forming a relatively closed water bath space between the centrifugal disc 510 and the water bath tank 710, reducing the evaporation of the water bath solution during the water bath process.

[0088] Reference Figure 1-20 The working steps of the PCR gene amplification system are as follows:

[0089] Preliminary manual preparation stage: The reaction materials required for gene amplification are loaded into PCR tubes and placed in the first placement well 211 of the first turntable 210 in the order of addition. The amount of each reaction material added, the centrifugation parameters and the water bath parameters are set by the master controller.

[0090] In the later automatic operation stage: the centrifuge tray 510 is moved to the left by a preset distance, and the PCR tube and centrifuge tube are opened by the opening and closing cap assembly 900 at the corresponding time period. The reaction materials in the PCR tube are added to the centrifuge tube 120 in a standardized manner by the pipetting device 300 and the head changing device 400. After the capping assembly 900 closes the cap 130 of the centrifuge tube 120, it moves the centrifuge disc back to the middle position on the right, and after the protective cover 610 is closed, it drives the first drive motor 521 to rotate the centrifuge disc 510. After the centrifuge tube 120 is centrifuged for a preset time, the reaction assembly prepares the water bath conditions, drives the protective cover 610 to continue to move down and the water bath 710 to move up, and performs water bath treatment on the centrifuge tube 120. By controlling the first drive motor 521 and the interception assembly 740, the centrifuge tube 120 is driven to switch the water bath chamber 730 to complete the switching of different reaction stages. After a preset number of cycles or a preset time of reaction, the protective cover 610 is driven to move up and the water bath 710 is driven to move down, and the centrifuge tube 120 is taken out, which can then proceed to the next step of processing.

[0091] It can be understood that, in this embodiment, all moving parts can acquire their position and attitude after movement by adding position sensors, thereby ensuring the coordination between different devices, components, and parts. All electrically powered or controlled parts are communicatively connected to the main controller to facilitate the regulation of the entire system.

[0092] The embodiments of the present invention have been described in detail above with reference to the accompanying drawings. However, the present invention is not limited to the above embodiments. Within the scope of knowledge possessed by those skilled in the art, various changes can be made without departing from the spirit of the present invention.

Claims

1. A PCR gene amplification system, characterized in that, include: A centrifugal assembly includes a centrifugal disc for holding centrifugal tubes and a first drive for rotating the centrifugal disc; The reaction assembly includes a water bath located below the centrifuge assembly and a second drive unit for adjusting the height of the water bath relative to the centrifuge disc. The water bath is provided with at least two partitions in the inner ring, which divide the water bath into at least two water bath chambers distributed in the ring. Each water bath chamber is provided with a temperature control device that can independently control the water bath temperature. Each partition has a notch for the centrifuge tube to pass through and an interception component for closing or opening the notch. After centrifuging the centrifuge tube containing the reactants into the centrifuge plate, the height of the water bath is adjusted so that the centrifuge tube enters one of the water bath chambers for a preset water bath treatment time. After the water bath treatment is completed, the centrifuge plate is rotated and the notch is opened, so that the centrifuge tube passes through the notch and enters the next water bath chamber with a different water bath temperature to react and then the notch is closed. The interception assembly includes at least two interception doors rotatably mounted on the partition and a third drive unit that drives each interception door to rotate independently. When the at least two interception doors rotate to close together, they form an assembly. When the interception doors on the same side of the partition are closed, they jointly seal the gap. The assembly forms a closed space that can accommodate the centrifuge tube. When the interception doors on the same side of the partition rotate, they can open the assembly to allow the centrifuge tube to enter and exit the closed space. The system also includes a protective component, which includes a protective cover above the centrifuge disc and a fourth driving component for closing or opening the centrifuge disc with the protective cover. The first driving component includes a first driving motor mounted on the top of the protective cover. The output end of the first driving motor is connected to a drive shaft extending into the protective cover. A drive ring is mounted at the bottom of the drive shaft. The centrifuge disc has a drive hole that matches the drive ring. When the fourth driving component closes or opens the centrifuge disc, the drive ring extends into the drive hole to drive the centrifuge disc to rotate, or extends out of the drive hole to disconnect the drive connection with the centrifuge disc. A first gear is coaxially mounted on the top of the drive shaft, and a second gear meshing with the first gear is mounted on the protective cover. The centrifugal disc is provided with a first gear ring adapted to the second gear. The first gear ring is located above the drive hole, and the centrifugal disc is provided with an idle hole that communicates with the drive hole and is located below the drive hole. When the protective cover moves down until the drive ring passes through the drive hole and enters the idle hole, the second gear meshes with the first gear ring to drive the centrifugal disc to rotate. The diameter of the first gear ring is larger than the diameter of the drive hole. When preparing for water bath treatment, as the water bath rises, the fourth drive component drives the protective cover to continue moving downwards, causing the transmission ring to pass through the transmission hole and enter the idle hole, so that the transmission ring no longer drives the centrifugal disc to rotate. At the same time, the second gear meshes with the first gear ring, and the first drive motor drives the centrifugal disc to rotate through the first gear, the second gear, and the first gear ring.

2. The PCR gene amplification system according to claim 1, characterized in that: The assembly includes four intercepting doors, two of which are rotatably mounted on one side wall of the notch, and the other two are rotatably mounted on the other side wall of the notch. When the two intercepting doors on the same side of the partition rotate in a direction away from or towards each other, the assembly opens or closes.

3. The PCR gene amplification system according to claim 1, characterized in that: The enclosed space is adapted to fit the centrifuge tube.

4. The PCR gene amplification system according to claim 1, characterized in that: The inner side of the protective cover is provided with a sealing edge, and the bottom of the sealing edge is provided with a sealing ring. When the protective cover moves down to the point where the second gear meshes with the first gear ring, the protective cover and the water bath are mutually sealed and covered, and the sealing ring abuts against the edge of the centrifuge plate.

5. The PCR gene amplification system according to claim 1, characterized in that: The system also includes a support cylinder for supporting the centrifugal disc. A limiting ring is provided at the bottom of the centrifugal disc and sleeved outside the support cylinder. A track wheel assembly and a fifth driving component for driving the track wheel assembly to rise and fall are provided inside the support cylinder. The track wheel assembly includes a support frame, two transmission wheels rotatably mounted on the support frame, a track sleeved on the two transmission wheels, and a sixth driving component for driving the track. The inner sides of the track are respectively engaged with the two transmission wheels. A rack that can engage with the outer side of the track is provided on the bottom surface of the centrifugal disc. An L-shaped slider is installed on the support frame. A groove is opened at the bottom of the centrifugal disc for the L-shaped slider to extend into and be limited to slide from one end. When the fifth driving component moves the support frame upward, the track meshes with the rack and lifts the centrifugal disc, causing the limiting ring to disengage from the support cylinder. At this time, the L-shaped slider is inserted into the groove, and the track is then driven to move the centrifugal disc horizontally. The L-shaped slider slides within the groove.

6. The PCR gene amplification system according to claim 1, characterized in that: The system also includes a material turntable device, a pipetting device, and a tip changing device. The material turntable device includes a first turntable and a seventh driving component for rotating the first turntable. The first turntable is provided with a plurality of first placement holes for placing PCR tubes. The pipetting device includes a pipette and a moving component for moving the pipette in the vertical and horizontal directions. The tip changing device includes a moving tray and an eighth driving component for moving the moving tray back and forth. The moving tray is provided with a tip box and a sharps box in sequence along the front-back direction. The first turntable, the moving tray, and the centrifuge tray are arranged side by side in the left-right direction. After the pipette is inserted into the tip box, it aspirates the reaction product in the PCR tube and adds it to the centrifuge tube. Then, the used tip is pushed into the sharps box.

7. The PCR gene amplification system according to claim 6, characterized in that: The system also includes a cap opening and closing assembly. The centrifuge tube and / or the PCR tube are reaction tubes with caps. One end of the cap is flexibly connected to the reaction tube, and the other end has a protrusion protruding from the reaction tube. The cap opening and closing assembly includes an extension plate and a ninth driving component for rotating the extension plate. The extension plate has a transverse groove for the protrusion to enter horizontally. The extension plate has an airflow channel. One end of the airflow channel is connected to a positive and negative gas source, and the other end forms a suction cup opening corresponding to the cap. When the protrusion of the cap is in the transverse groove, the positive and negative gas sources provide negative pressure, causing the cap to be adsorbed at the suction cup opening. Starting the extension plate to rotate can rotate the cap to remove or insert it into the reaction tube.

Images