A totally enclosed corrugated sampler

Abstract

The application discloses a full-closed corrugated sampler, relates to the field of biological medicines, and comprises a sampler body, wherein the sampler body comprises a connecting part and an extension part, the connecting part is connected to an interface, a closed section is formed on the connecting part, the extension part is arranged on the connecting part, and a sampling strip is arranged in the extension part. Before cell culture, the connecting part is sealed and connected to the interface of a fixed bed reactor, the extension part is in a contraction state, and the end of the sampling strip is located in the fixed bed reactor; when sampling is needed during the culture process, the extension part is stretched, the extension part is elongated and drives the sampling strip to move out of the fixed bed reactor, the end of the sampling strip enters the extension part through the connecting part, the connecting part is blocked through the closed section, the connecting part is cut through the closed section, and sampling can be completed under full-closed conditions, so that the fixed bed reactor is conveniently sampled.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of biological medicine, in particular to a full-closed corrugated sampler. BACKGROUND

[0002] The fixed bed bioreactor is a device specially used for the large-scale culture of adherent cells. Its core principle is to realize the high-density expansion of adherent cells by anchoring cells on the surface of a specific carrier. This type of reactor has the ability to monitor and control key process parameters such as pH, DO and temperature online, and is widely used in the fields of virus vector preparation, vaccine production and large-scale exosome preparation.

[0003] Compared with the bioreactor based on suspension culture, the fixed bed reactor has the technical bottleneck of difficult cell sampling during the culture process. Especially when using sheet or roll membrane type immobilized carriers, the current operation mode often chooses to completely avoid sampling or needs to open a temporary opening on the reactor structure for open sampling. Such operation not only significantly increases the risk of microbial contamination, but also has potential biosafety hazards caused by exposure of pathogens for processes involving highly pathogenic viruses. SUMMARY

[0004] In order to facilitate sampling of the fixed bed reactor, the present application provides a full-closed corrugated sampler.

[0005] The full-closed corrugated sampler provided by the present application adopts the following technical solution:

[0006] A full-closed corrugated sampler, comprising a sampler body, the sampler body comprising a connecting part and an extension part, the connecting part being sealingly connected to an interface of a fixed bed reactor, the extension part being provided on the connecting part, a sampling strip being provided in the extension part, one end of the sampling strip being fixedly connected to the extension end of the extension part, when the extension part is contracted, the other end of the sampling strip passes through the connecting part and extends into the fixed bed reactor through the interface, when the extension part is stretched, the other end of the sampling strip moves from the fixed bed reactor into the extension part, a closed section being formed on the connecting part, the closed section being used to block the connecting part and divide the connecting part into two parts.

[0007] By adopting the above technical solution, before cell culture, the connecting part is sealingly connected to the interface of the fixed bed reactor, the extension part is in a contracted state, and the end of the sampling strip is located in the fixed bed reactor. When sampling is needed during the culture process, the extension part is stretched, the extension part is elongated and drives the sampling strip to move out of the fixed bed reactor, the end of the sampling strip enters the extension part through the connecting part, and the connecting part is blocked by the closed section. The connecting part is cut by the closed section, and sampling is completed under full-closed conditions, thereby facilitating sampling of the fixed bed reactor.

[0008] Preferably, the connecting part is a plastic connecting pipe, and the telescopic part is a plastic bellows.

[0009] By adopting the above technical scheme, before cell culture, the connecting pipe is sealingly connected to the interface of the fixed bed reactor, the bellows is in a contracted state, and the end of the sampling strip is located in the fixed bed reactor; when sampling is needed during the culture process, the bellows is stretched, the bellows is elongated and drives the sampling strip to move out of the fixed bed reactor, the end of the sampling strip enters the bellows through the connecting pipe, the sealing section of the connecting pipe is welded and blocked by using a pipe sealing machine, the connecting pipe is cut at the welding position and divided into two, and sampling can be completed under a completely closed condition.

[0010] Preferably, a limiting clamp is detachably arranged on the telescopic part, the limiting clamp limits the telescopic part and makes the telescopic part in a contracted state.

[0011] By adopting the above technical scheme, after the connecting pipe is sealingly connected to the interface of the fixed bed reactor, the bellows is clamped and limited by using the limiting member, so that the bellows can always be kept in a contracted state, and thus the sampling strip can always be located in the fixed bed reactor; when the bellows needs to be stretched, the limiting clamp is detached and removed, and the bellows can be elongated.

[0012] Preferably, an end of the telescopic part away from the connecting part is detachably provided with a plug, and one end of the sampling strip is fixedly connected with the plug.

[0013] By adopting the above technical scheme, after the sampler completes sampling, the plug is detached and removed from the telescopic part, and the plug can drive the sampling strip to move out of the telescopic part, so that the sampling strip can be conveniently taken out.

[0014] Preferably, the connecting part is provided with an expansion pipe on a side close to the interface of the sealing section.

[0015] By adopting the above technical scheme, when the telescopic part is elongated and the end of the sampling strip enters the telescopic part through the connecting part, the expansion pipe of the connecting part is elongated, so that the sealing section on the connecting part is away from the interface, and the sealing section can be conveniently closed.

[0016] Preferably, the connecting part comprises an upper tube and a lower tube, the lower tube is detachably connected to the interface of the fixed bed reactor, the upper tube is detachably connected to the lower tube, the telescopic part comprises a sleeve and a tip, the sleeve is slidably sleeved on the upper tube, the tip is detachably arranged on the sleeve, one end of the sampling strip is connected with the tip; the sealing section is located at the connection of the upper tube and the lower tube, a second sealing assembly is arranged in the end of the upper tube close to the lower tube, a first sealing assembly is arranged in the end of the lower tube close to the upper tube, the sampling strip passes through the second sealing assembly and the first sealing assembly and extends into the fixed bed reactor, when the sleeve drives the sampling strip to move into the upper tube and the upper tube is detached from the lower tube, the second sealing assembly seals the opening of the upper tube, and the first sealing assembly seals the opening of the lower tube.

[0017] By adopting the above technical scheme, before cell culture, the lower tube is sealed and connected to the interface of the fixed bed reactor, then the upper tube is installed on the lower tube, subsequently the sleeve is slidably sleeved on the upper tube, and finally the tip is installed on the upper tube, so that the sampling strip passes through the second sealing assembly and the first sealing assembly and extends into the fixed bed reactor, when sampling is needed during the culture process, the sleeve is slid upward, the sleeve drives the sampling strip to move out of the fixed bed reactor through the tip, the end of the sampling strip moves into the upper tube, then the upper tube is detached from the lower tube, the second sealing assembly automatically seals the opening of the upper tube, and the first sealing assembly automatically seals the opening of the lower tube, so that sampling can be completed under a completely sealed condition.

[0018] Preferably, the first sealing assembly comprises a first mounting ring, a first sliding plate, a first rotating plate, a first blocking ring, a first pushing ring and a plurality of first elastic members, the first mounting ring is fixedly arranged on the inner wall of the lower tube, the plurality of first elastic members are arranged on the first mounting ring, the first sliding plate is slidably arranged in the lower tube and abuts against the plurality of first elastic members, a first sliding block is arranged on the side wall of the first sliding plate, a first sliding groove is formed in the lower tube along the axis direction of the lower tube, the first sliding block is slidably arranged in the first sliding groove, the first rotating plate is rotatably arranged on the first sliding plate, a first rotating block is arranged on the side wall of the first rotating plate, a first rotating groove is obliquely formed in the inner side wall of the lower tube, the first rotating block is slidably arranged in the first rotating groove, a first through hole is eccentrically formed in the first sliding plate and the first rotating plate, the sampling strip passes through the first through hole, the first blocking ring is fixedly arranged on the inner side wall of the lower tube and located on the side of the first rotating plate away from the first sliding plate, the first rotating plate moves to abut against the first blocking ring, and the first pushing ring is fixedly arranged on the end of the upper tube and moves to abut against the first rotating plate.

[0019] When the upper pipe body is separated from the lower pipe body, the plurality of first elastic members push the first sliding plate and the first rotating plate to move towards the first blocking ring, the first sliding plate does not rotate during the movement under the action of the first sliding block and the first sliding groove, the first rotating plate rotates during the movement under the action of the first rotating block and the first rotating groove, the first perforations on the first sliding plate and the first rotating plate are not communicated with each other, and the first rotating plate is moved to abut against the first blocking ring, so that the opening of the lower pipe body is blocked.

[0020] Preferably, the second closing assembly comprises a second mounting ring, a second sliding plate, a second rotating plate, a second blocking ring, a second pushing ring and a plurality of second elastic members, the second mounting ring is fixedly arranged on the inner wall of the upper pipe body, the plurality of second elastic members are arranged on the second mounting ring, the second sliding plate is slidingly arranged in the upper pipe body and abuts against the plurality of second elastic members, a second sliding block is arranged on the side wall of the second sliding plate, a second sliding groove is arranged in the upper pipe body along the axis direction of the upper pipe body, the second sliding block is slidingly arranged in the second sliding groove, the second rotating plate is rotationally arranged on the second sliding plate, a second rotating block is arranged on the side wall of the second rotating plate, a second rotating groove is obliquely arranged on the inner wall of the upper pipe body, the second rotating block is slidingly arranged in the second rotating groove, a second perforation is eccentrically arranged in the second sliding plate and the second rotating plate, the sampling strip passes through the second perforation, the second blocking ring is fixedly arranged on the side of the second rotating plate away from the second sliding plate, the second rotating plate moves to abut against the second blocking ring, and the second pushing ring is fixedly arranged on the second rotating plate.

[0021] When the upper pipe body is separated from the lower pipe body, the plurality of second elastic members push the second sliding plate and the second rotating plate to move towards the second blocking ring, the second sliding plate does not rotate during the movement under the action of the second sliding block and the second sliding groove, the second rotating plate rotates during the movement under the action of the second rotating block and the second rotating groove, the second perforations on the second sliding plate and the second rotating plate are not communicated with each other, and the second rotating plate is moved to abut against the second blocking ring, so that the opening of the upper pipe body is blocked.

[0022] Preferably, a guide rod is arranged on the side wall of the sleeve, and a guide groove is arranged on the outer side wall of the upper pipe body along the self-axis direction, and the guide rod is slidingly arranged in the guide groove.

[0023] By using the above technical scheme, when the sleeve slides on the upper pipe body, the sleeve drives the guide rod to slide in the guide groove, and the guide rod cooperates with the guide groove, so that the sleeve does not rotate during sliding, thereby making the end head drive the sampling strip to move more stably.

[0024] Preferably, a third elastic member is arranged on the sleeve, the third elastic member pushes the guide rod to move towards the upper pipe body, and a clamping groove is arranged on the top of the upper pipe body and communicates with the guide groove.

[0025] By using the above technical scheme, when the sleeve drives the sampling strip to move into the upper pipe body through the end head, the sleeve drives the guide rod to move to the clamping groove, and the third elastic member pushes the guide rod to slide and insert into the clamping groove, thereby locking the position of the sleeve, facilitating subsequent disassembly of the upper pipe body.

[0026] Preferably, a slide is arranged on the bottom wall of the end head, a lock block is slidingly arranged in the slide, a lock needle is fixedly arranged on the side wall of the lock block, and the lock needle penetrates the sampling strip and inserts into the end head.

[0027] By using the above technical scheme, after the upper pipe body is disassembled, the end head is disassembled and removed from the sleeve, the lock block is slid, and the lock needle is driven out of the end head, so that the sampling strip can be quickly disassembled and removed from the end head.

[0028] In summary, the present application has at least one of the following beneficial technical effects:

[0029] 1. The sampling device body is used, before cell culture, the connecting part is sealingly connected to the interface of the fixed bed reactor, the telescopic part is in the contracted state, and the end of the sampling strip is located in the fixed bed reactor, when sampling is needed during the culture process, the telescopic part is stretched, the telescopic part is elongated and drives the sampling strip to move out of the fixed bed reactor, the end of the sampling strip enters the telescopic part through the connecting part, and the connecting part is blocked through the closing section, the connecting part is cut through the closing section, and sampling can be completed under full sealing condition, thereby facilitating sampling of the fixed bed reactor.

[0030] 2. The limiting clamp is used, after the connecting pipe is sealingly connected to the interface of the fixed bed reactor, the limiting member is used to clamp and limit the corrugated pipe, so that the corrugated pipe can always remain in the contracted state, thereby enabling the sampling strip to always be located in the fixed bed reactor, when the corrugated pipe needs to be stretched, the limiting clamp is disassembled and removed, and the corrugated pipe can be lengthened.

[0031] 3. By the guide rod, when the sleeve slides on the upper tube, the sleeve drives the guide rod to slide in the guide groove, the guide rod cooperates with the guide groove, so that the sleeve does not rotate in the sliding process, so that the end head drives the sampling strip to move more stably. BRIEF DESCRIPTION OF DRAWINGS

[0032] Figure 1 It is a schematic diagram of the overall structure of the full-closed corrugated sampler in embodiment 1 of the present application, which highlights the retracted state of the sampler.

[0033] Figure 2 It is a schematic diagram of the overall structure of the full-closed corrugated sampler in embodiment 1 of the present application, which highlights the stretched state of the sampler.

[0034] Figure 3 It is a schematic diagram of the overall structure of the full-closed corrugated sampler in embodiment 1 of the present application, which highlights the closed state of the connecting part.

[0035] Figure 4 It is a schematic diagram of the overall structure of the full-closed corrugated sampler in embodiment 2 of the present application.

[0036] Figure 5 It is a schematic diagram of the overall structure of the full-closed corrugated sampler in embodiment 3 of the present application.

[0037] Figure 6 It is a schematic diagram of the overall structure of the full-closed corrugated sampler in embodiment 4 of the present application.

[0038] Figure 7 It is a sectional view of the overall structure of the full-closed corrugated sampler in embodiment 4 of the present application, which highlights the connection state of the upper tube and the lower tube.

[0039] Figure 8 It is an enlarged schematic diagram of A in the present application. Figure 7

[0040] It is an enlarged schematic diagram of B in the present application. Figure 9 Figure 7 It is an enlarged schematic diagram of C in the present application.

[0041] Figure 10 Figure 7 It is an enlarged schematic diagram of C in the present application.

[0042] Figure 11 It is a forward explosion sectional view of part of the structure of the full-closed corrugated sampler in embodiment 4 of the present application.

[0043] Figure 12 It is a reverse explosion sectional view of part of the structure of the full-closed corrugated sampler in embodiment 4 of the present application.

[0044] Figure 13 ​​It is the overall structure explosion section view of the full-closed corrugated sampler in the embodiment 4 of the present application, for highlighting the separated state of the upper pipe body and the lower pipe body;

[0045] Figure 14 It is the enlarged schematic view at D in the embodiment 4 of the present application; Figure 13

[0046] Figure 15 It is the enlarged schematic view at E in the embodiment 4 of the present application; Figure 13

[0047] Figure 16 It is the enlarged schematic view at F in the embodiment 4 of the present application. Figure 13

[0048] Reference signs: 1, sampler body; 11, connecting part; 111, upper pipe body; 112, lower pipe body; 12, telescopic part; 121, sleeve; 122, end head; 2, interface; 3, sampling strip; 4, closed section; 5, limiting clamp; 6, first closing assembly; 61, first mounting ring; 62, first sliding plate; 63, first rotating plate; 64, first blocking ring; 65, first pushing ring; 66, first elastic member; 7, second closing assembly; 71, second mounting ring; 72, second sliding plate; 73, second rotating plate; 74, second blocking ring; 75, second pushing ring; 76, second elastic member; 8, first sliding block; 9, first sliding groove; 10, first rotating block; 13, first rotating groove; 14, first through hole; 15, second sliding block; 16, second sliding groove; 17, second rotating block; 18, second rotating groove; 19, second through hole; 20, guide rod; 21, guide groove; 23, clamping groove; 24, sliding way; 25, locking block; 26, locking pin; 27, plug; 28, third elastic member; 29, fourth elastic member; 30, telescopic pipe. DETAILED DESCRIPTION

[0049] The following will be combined with the Figures 1-16 The present application will be further described in detail.

[0050] The embodiment of the present application discloses a full-closed corrugated sampler.

[0051] Embodiment 1:

[0052] Referring to Figure 1 and Figure 2 , a full-closed corrugated sampler comprises a sampler body 1, the sampler body 1 is composed of a connecting part 11 and a telescopic part 12, in the present application, the connecting part 11 can be selected as a plastic connecting pipe, and the telescopic part 12 can be selected as a plastic corrugated pipe. The end parts of the connecting part 11 and the telescopic part 12 fixedly connected and mutually communicated, the end part of the connecting part 11 away from the telescopic part 12 is sealingly connected on the interface 2 at the top of the fixed bed reactor.

[0053] ​​​The telescopic part 12 is provided with a sampling strip 3, the top end of the sampling strip 3 is fixedly connected to the end of the telescopic part 12 away from the connecting part 11, in the present application, the fixing mode of the telescopic part 12 and the sampling strip 3 can be selected as tube sealing machine welding fixation or Robert tube clamp clamping fixation.

[0054] The bottom end position of the sampling strip 3 is directly controlled by the state of the telescopic part 12. When the telescopic part 12 is in the contracted state (refer to Figure 1 ), the sampling strip 3 is driven by the telescopic part 12, the bottom end of the sampling strip 3 penetrates through the connecting part 11 and is pushed downward through the interface 2, and finally reaches and is exposed to the inside of the fixed bed reactor, which is the initial sampling position. When the telescopic part 12 is in the stretched state (refer to Figure 2 ), the sampling strip 3 is withdrawn, the bottom end of the sampling strip 3 moves upward from the inside of the fixed bed reactor, and is completely withdrawn and accommodated in the cavity of the telescopic part 12, which is the end sampling position.

[0055] The telescopic part 12 is provided with a limiting clamp 5, the limiting clamp 5 is composed of two clamp frames, and the two clamp frames are detachably connected. When the two clamp frames are connected, the limiting clamp 5 blocks the two ends of the telescopic part 12, so that the telescopic part 12 can always be in the contracted state; when the two clamp frames are separated, the limiting clamp 5 releases the restriction on the telescopic part 12, so that the telescopic part 12 can be elongated.

[0056] Referring to Figure 3 , the middle part of the connecting part 11 is formed with a closed section 4, the closed section 4 is used to block the connecting part 11 and divide the connecting part 11 into two parts. The closed section 4 of the connecting part 11 can be closed by using a tube sealing machine or a Robert tube clamp, and after the closed section 4 is cut, the telescopic part 12 can be taken off from the connecting part 11.

[0057] The implementation principle of a fully enclosed corrugated sampler according to an embodiment of this application is as follows: The sampler is for single use. Before cell culture begins, the connecting part 11 is sealed to the interface 2 of the fixed bed reactor, the telescopic part 12 is in a contracted state, and the limiting clamp 5 is installed on the telescopic part 12 to ensure that the bottom end of the sampling strip 3 is placed inside the fixed bed reactor beforehand and is in full contact with the culture environment. When sampling and testing are required during the culture process, the operator can remove the limiting clamp 5 and stretch the telescopic part 12, causing it to change from a contracted state to a stretched state. During the stretching process, the built-in sampling strip 3 is smoothly moved out of the fixed bed reactor. During this process, the bottom end of the sampling strip 3 with the sample attached will pass through the connecting part 11 and be retracted into the cavity of the telescopic part 12. Subsequently, the sealing section 4 on the connecting part 11 is sealed and blocked by a tube sealing machine or Robert clamp to form an effective seal and isolation. Then, the connecting part 11 is cut at the sealing section 4. This operation ensures that while sampling strip 3 is successfully extracted, the interior of the fixed-bed reactor remains completely isolated from the external environment, eliminating any risk of contamination. Ultimately, this achieves safe and convenient acquisition of culture samples under completely sterile and sealed conditions.

[0058] Example 2:

[0059] Reference Figure 4 The difference between this embodiment and embodiment 1 is that a plug 27 is installed at the end of the telescopic part 12 away from the connecting part 11, and the top end of the sampling strip 3 is fixedly connected to the plug 27. The plug 27 is detachably and sealed to the telescopic part 12 by means of threaded connection, plug-in connection or other means.

[0060] The implementation principle of Embodiment 2 of this application is as follows: During the extension and retraction process, the telescopic part 12 drives the plug 27 to move, and the plug 27 drives the sampling strip 3 to move. After the sampler completes sampling, the plug 27 is removed from the telescopic part 12, and the plug 27 can drive the sampling strip 3 to be taken out from the telescopic part 12, thereby facilitating the removal of the sampling strip 3.

[0061] Example 3:

[0062] Reference Figure 5 The difference between this embodiment and embodiment 1 is that a telescopic tube 30 is formed on the connecting part 11. In this application, the telescopic tube 30 can be a corrugated pipe, and the telescopic tube 30 is located between the closed section and the interface 2.

[0063] The implementation principle of Embodiment 3 of this application is as follows: When using a sealing machine to weld and seal the sealing section 4, the large size of the sealing machine requires a large length for the connecting part 11 to be used to seal the sealing section 4. In this embodiment, the telescopic tube 30 on the connecting part 11 allows the length of the connecting part 11 to be adjusted. When it is necessary to seal the sealing section 4, the length of the connecting part 11 can be increased by stretching the telescopic tube 30, thereby facilitating the sealing of the sealing section 4 using the sealing machine.

[0064] Example 4:

[0065] Reference Figure 6 The difference between this embodiment and Embodiment 1 is that the connecting part 11 includes an upper tube 111 and a lower tube 112, and the telescopic part 12 includes a sleeve 121 and an end 122. The lower tube 112 is detachably and sealingly connected to the interface 2 of the fixed bed reactor, and the upper tube 111 is coaxially and detachably installed at the top end of the lower tube 112 by means of threads or plug-in connection. The sealing section 4 is located at the connection between the upper tube 111 and the lower tube 112.

[0066] Reference Figure 7 and Figure 8 The sleeve 121 is slidably fitted onto the upper tube body 111, and the end 122 is detachably installed on the top of the sleeve 121 by means of thread or plug-in connection. A slide 24 is provided on the bottom wall of the end 122, and a locking block 25 is slidably installed on the bottom of the end 122 within the slide 24. Multiple locking pins 26 are fixedly installed on one side wall of the locking block 25. A fourth elastic element 29 is installed in the slide 24 on the side of the locking block 25 away from the locking pins 26. In this application, the fourth elastic element 29 can be a spring. The fourth elastic element 29 pushes the locking block 25 to slide within the slide 24, and the locking block 25 drives the multiple locking pins 26 to move through the top of the sampling strip 3 and insert into the end 122, thereby fixing the sampling strip 3 to the end 122.

[0067] Reference Figure 7 and Figure 9 Two guide rods 20 are symmetrically installed on the bottom side wall of the sleeve 121 along its diameter. Two guide grooves 21 are formed on the outer side wall of the upper tube 111 along its length, and the two guide rods 20 are slidably installed within these grooves. When the sleeve 121 slides up and down on the upper tube 111, it drives the two guide rods 20 to slide within the two guide grooves 21, thereby guiding the sliding of the sleeve 121 and preventing rotation during its movement.

[0068] Two third elastic elements 28 are symmetrically installed on the bottom of the outer wall of the sleeve 121 along its own diameter direction. The two third elastic elements 28 are respectively sleeved on the two guide rods 20. The cross-section of the guide rod 20 is T-shaped. The two ends of the third elastic elements 28 are fixedly connected to the sleeve 121 and the guide rod 20 respectively. In this application, the third elastic element 28 can be a tension spring, and the third elastic element 28 pulls the guide rod 20 to move closer to the upper tube body 111.

[0069] Reference Figure 8 and Figure 9 A retaining groove 23 is formed on the outer wall of the sleeve 121 at the top of the guide groove 21. The retaining groove 23 communicates with the guide groove 21, and the depth of the retaining groove 23 is greater than the depth of the guide groove 21. When the sleeve 121 moves upward to the top of the upper tube 111, the sleeve 121 drives the guide post to move to the retaining groove 23. Under the action of the third elastic element 28, the guide rod 20 extends into the retaining groove 23 (see reference). Figure 13 and Figure 14 This allows the sleeve 121 to be limited, preventing it from moving further on the upper tube 111, thus facilitating the subsequent separation of the upper tube 111 and the lower tube 112.

[0070] Reference Figure 7 , Figure 10 , Figure 11 and Figure 12 A first sealing component 6 is installed at the top of the inner cavity of the lower tube 112, and a second sealing component 7 is installed at the bottom of the inner cavity of the upper tube 111. When the upper tube 111 is connected to the lower tube 112, the sampling strip 3 can pass through the second sealing component 7 and the first sealing component 6 and extend into the fixed bed reactor through the interface 2; when the upper tube 111 is separated from the lower tube 112, the second sealing component 7 can close the opening of the upper tube 111, and the first sealing component 6 can close the opening of the lower tube 112.

[0071] Specifically, the first sealing component 6 includes a first mounting ring 61, a first sliding plate 62, a first rotating plate 63, a first retaining ring 64, a first push ring 65, and eight first elastic elements 66. The first mounting ring 61 is fixedly mounted on the inner side wall of the lower tube body 112, and the eight first elastic elements 66 are mounted at equal intervals along the circumference of the first mounting ring 61 on the top wall of the first mounting ring 61. In this application, the first elastic elements 66 can be selected as springs.

[0072] The first slide plate 62 is slidably installed inside the lower tube 112, and the bottom wall of the first slide plate 62 abuts against eight first elastic elements 66. A first slider 8 is fixedly installed on the outer wall of the first slide plate 62, and a first groove 9 is formed in the lower tube 112 along its own axial direction, and the first slider 8 is slidably installed in the first groove 9.

[0073] Reference Figure 11 andFigure 12 The first rotating plate 63 is slidably installed inside the lower tube body 112. The first rotating plate 63 is located on the side of the first sliding plate 62 away from the first elastic member 66. The first rotating block 10 is fixedly installed on the outer wall of the first rotating plate 63. The first rotating groove 13 is opened on the inner wall of the lower tube body 112. The first rotating groove 13 is opened at an inclination along the inner wall of the lower tube body 112. The first rotating block 10 is slidably installed in the first rotating groove 13.

[0074] Both the first sliding plate 62 and the first rotating plate 63 are circular plates. A first through hole 14 is eccentrically formed inside both the first sliding plate 62 and the first rotating plate 63, allowing the sampling strip 3 to pass through the first through hole 14 and through the first sliding plate 62 and the first rotating plate 63. A first retaining ring 64 is fixedly installed on the top of the inner wall of the lower tube 112, located on the side of the first rotating plate 63 away from the first sliding plate 62. A first push ring 65 is fixedly installed at the bottom end of the upper tube 111, located inside the first retaining ring 64.

[0075] When the upper tube 111 and the lower tube 112 separate, the eight first elastic elements 66 generate a thrust, causing the first sliding plate 62 and the first rotating plate 63 to move together toward the first retaining ring 64. The first sliding plate 62 remains stable and does not rotate during the movement due to the limitation imposed by the first slider 8 and the first groove 9, while the first rotating plate 63 rotates synchronously by 120° under the action of the first rotating block 10 and the first rotating groove 13. Ultimately, the first through holes 14 on the first sliding plate 62 and the first rotating plate 63 will be misaligned and no longer connected due to the rotation of the first rotating plate 63 (see reference). Figure 16 The opening of the lower pipe 112 can be sealed when the first rotating plate 63 moves to a position where it is in close contact with the first retaining ring 64.

[0076] When the upper tube 111 and the lower tube 112 are assembled, the upper tube 111 will drive the first push ring 65 to move during the installation process. The first push ring 65 will then apply a pushing force to the first rotating plate 63 and the first sliding plate 62, causing them to move towards the first mounting ring 61. During this process, the first sliding plate 62 will squeeze the first elastic element 66, causing the first elastic element 66 to contract and deform; at the same time, the first rotating plate 63 will rotate during sliding, ultimately allowing the first sliding plate 62 to be precisely aligned with the first through hole 14 on the first rotating plate 63 (see reference). Figure 10 At this point, the sampling strip 3 can pass smoothly through the first perforation 14 through the entire first closed component 6.

[0077] Reference Figure 7 , Figure 10 , Figure 11 and Figure 12The second sealing assembly 7 includes a second mounting ring 71, a second sliding plate 72, a second rotating plate 73, a second retaining ring 74, a second push ring 75, and eight second elastic elements 76. The second mounting ring 71 is fixedly mounted on the inner side wall of the upper tube 111, and the eight second elastic elements 76 are fixedly mounted at equal intervals along the circumference of the second mounting ring 71 on the bottom wall of the second mounting ring 71. In this application, the second elastic elements 76 can be selected as springs.

[0078] The second slide plate 72 is slidably installed inside the upper tube 111, and the top wall of the second slide plate 72 abuts against eight second elastic elements 76. A second slider 15 is fixedly installed on the outer wall of the second slide plate 72, and a second groove 16 is opened on the inner wall of the first push ring 65 along its own axial direction, and the second slider 15 is slidably installed in the second groove 16.

[0079] Reference Figure 11 and Figure 12 The second rotating plate 73 is slidably installed inside the upper tube 111. The second rotating plate 73 is located on the side of the second sliding plate 72 away from the second elastic member 76. A second rotating block 17 is fixedly installed on the outer wall of the second rotating plate 73. A second rotating groove 18 is opened on the inner wall of the upper tube 111. The second rotating groove 18 is opened obliquely along the inner wall of the upper tube 111. The second rotating block 17 is slidably installed in the second rotating groove 18.

[0080] Both the second slide plate 72 and the second rotating plate 73 are circular plates. A second through hole 19 is eccentrically formed inside both the second slide plate 72 and the second rotating plate 73, allowing the sampling strip 3 to pass through the second through hole 19 and through the second slide plate 72 and the second rotating plate 73. A second retaining ring 74 is fixedly installed on the bottom of the inner wall of the upper tube 111, located on the side of the second rotating plate 73 away from the second slide plate 72. A second push ring 75 is fixedly installed on the bottom wall of the second rotating plate 73, and moves to abut against the first rotating plate 63.

[0081] When the upper tube 111 and the lower tube 112 separate, the eight second elastic elements 76 generate a thrust, causing the second sliding plate 72 and the second rotating plate 73 to move together toward the second retaining ring 74. The second sliding plate 72 remains stable and does not rotate during the movement due to the limiting effect of the second slider 15 and the second sliding groove 16, while the second rotating plate 73 rotates synchronously by 120° under the action of the second rotating block 17 and the second rotating groove 18. Ultimately, the second through holes 19 on the second sliding plate 72 and the second rotating plate 73 will be misaligned and no longer connected due to the rotation of the second rotating plate 73 (see reference). Figure 15 The opening of the upper tube 111 can be sealed when the second rotating plate 73 moves to a position where it is in close contact with the second retaining ring 74.

[0082] When the upper tube 111 and the lower tube 112 are assembled, the upper tube 111 will drive the second push ring 75 to move and abut against the first rotating plate 63 during the installation process. The second push ring 75 will then apply a pushing force to the second rotating plate 73 and the second sliding plate 72, causing them to move towards the second mounting ring 71. During this process, the second sliding plate 72 will squeeze the second elastic element 76, causing the second elastic element 76 to contract and deform; at the same time, the second rotating plate 73 will rotate during sliding, ultimately allowing the second sliding plate 72 to be precisely aligned with the second through hole 19 on the second rotating plate 73 (see reference). Figure 10 At this point, the sampling strip 3 can pass smoothly through the second perforation 19 through the entire second closed component 7.

[0083] The implementation principle of Embodiment 4 of this application is as follows: the sampler can be reused after disassembly and cleaning. Before cell culture is started, the lower tube 112 needs to be sealed and connected to the interface 2 of the fixed bed reactor. Then, the upper tube 111 is installed on the lower tube 112. At this time, the first perforation 14 and the second perforation 19 in the first sealing component 6 and the second sealing component 7 are opened. Then, the sleeve 121 is slidably sleeved on the outside of the upper tube 111. Finally, the end 122 is installed and the position of the sampling strip 3 is adjusted so that the sampling strip 3 passes through the second sealing component 7 and the first sealing component 6 in sequence until it extends into the interior of the fixed bed reactor. When sampling is required during the cultivation process, the sleeve 121 is slid upward, and the sleeve 121 will move the sampling strip 3 out of the reactor through the end 122. After the end of the sampling strip 3 enters the upper tube 111, the upper tube 111 is removed from the lower tube 112. At this time, the second sealing component 7 will automatically seal the opening of the upper tube 111, and the first sealing component 6 will automatically seal the opening of the lower tube 112, thereby completing the sampling under fully sealed conditions.

[0084] The above are merely optional embodiments of this disclosure and are not intended to limit this disclosure. Various modifications and variations can be made to this disclosure by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this disclosure should be included within the scope of protection of this disclosure.

Claims

1. A fully enclosed corrugated sampler, characterized in that: The sampler includes a sampler body (1), which includes a connecting part (11) and a telescopic part (12). The connecting part (11) is sealed to the interface (2) of the fixed bed reactor. The telescopic part (12) is provided on the connecting part (11). A sampling strip (3) is provided inside the telescopic part (12). One end of the sampling strip (3) is fixedly connected to the telescopic end of the telescopic part (12). When the telescopic part (12) contracts, the other end of the sampling strip (3) passes through the connecting part (11) and extends into the fixed bed reactor through the interface (2). When the telescopic part (12) stretches, the other end of the sampling strip (3) moves from the fixed bed reactor into the telescopic part (12). A closed section (4) is formed on the connecting part (11). The closed section (4) is used to block the connecting part (11) and divide the connecting part (11) into two. The connecting part (11) includes an upper tube body (111) and a lower tube body (112). The lower tube body (112) is detachably connected to the interface (2) of the fixed bed reactor. The upper tube body (111) is detachably connected to the lower tube body (112). The telescopic part (12) includes a sleeve (121) and an end (122). The sleeve (121) is slidably fitted on the upper tube body (111). The end (122) is detachably mounted on the sleeve (121). One end of the sampling strip (3) is connected to the end (122). The closed section (4) is located at the connection between the upper tube body (111) and the lower tube body (112). The upper tube (111) is provided with a second sealing component (7) at one end near the lower tube (112), and the lower tube (112) is provided with a first sealing component (6) at one end near the upper tube (111). The sampling strip (3) passes through the second sealing component (7) and the first sealing component (6) and extends into the fixed bed reactor. When the sleeve (121) moves the sampling strip (3) into the upper tube (111) and the upper tube (111) is removed from the lower tube (112), the second sealing component (7) closes the opening of the upper tube (111), and the first sealing component (6) closes the opening of the lower tube (112). The first sealing component (6) includes a first mounting ring (61), a first sliding plate (62), a first rotating plate (63), a first retaining ring (64), a first pushing ring (65), and a plurality of first elastic elements (66). The first mounting ring (61) is fixedly disposed on the inner wall of the lower tube body (112). The plurality of first elastic elements (66) are disposed on the first mounting ring (61). The first sliding plate (62) is slidably disposed in the lower tube body (112) and abuts against the plurality of first elastic elements (66). A first slider (8) is disposed on the side wall of the first sliding plate (62). A first groove (9) is opened in the lower tube body (112) along its own axis. The first slider (8) is slidably disposed in the first groove (9). The first rotating plate (63) is rotatably disposed on the first sliding plate (64). 2) On the side wall of the first rotating plate (63), a first rotating block (10) is provided. The inner side wall of the lower tube (112) is inclinedly provided with a first rotating groove (13). The first rotating block (10) is slidably disposed in the first rotating groove (13). The first sliding plate (62) and the first rotating plate (63) are both eccentrically provided with a first through hole (14). The sampling strip (3) passes through the first through hole (14). The first retaining ring (64) is fixedly disposed on the inner side wall of the lower tube (112) and located on the side of the first rotating plate (63) away from the first sliding plate (62). The first rotating plate (63) moves to abut against the first retaining ring (64). The first push ring (65) is fixedly disposed at the end of the upper tube (111). The first push ring (65) moves to abut against the first rotating plate (63). The second sealing assembly (7) includes a second mounting ring (71), a second sliding plate (72), a second rotating plate (73), a second retaining ring (74), a second push ring (75), and a plurality of second elastic elements (76). The second mounting ring (71) is fixedly disposed on the inner wall of the upper tube (111), and the plurality of second elastic elements (76) are disposed on the second mounting ring (71). The second sliding plate (72) is slidably disposed in the upper tube (111) and abuts against the plurality of second elastic elements (76). A second slider (15) is disposed on the side wall of the second sliding plate (72). A second groove (16) is opened in the upper tube (111) along its own axis direction. The second slider (15) is slidably disposed in the second groove (16). The second rotating plate (73) The second rotating plate (73) is rotatably mounted on the second sliding plate (72). The second rotating block (17) is provided on the side wall of the second rotating plate (73). The inner side wall of the upper tube (111) is inclinedly provided with a second rotating groove (18). The second rotating block (17) is slidably mounted in the second rotating groove (18). The second sliding plate (72) and the second rotating plate (73) are both eccentrically provided with a second through hole (19). The sampling strip (3) passes through the second through hole (19). The second retaining ring (74) is fixedly mounted on the side of the second rotating plate (73) away from the second sliding plate (72). The second rotating plate (73) moves to abut against the second retaining ring (74). The second push ring (75) is fixedly mounted on the second rotating plate (73). The second push ring (75) moves to abut against the first rotating plate (63).

2. The fully enclosed corrugated sampler according to claim 1, characterized in that: The connecting part (11) is a plastic connecting pipe, and the telescopic part (12) is a plastic corrugated pipe.

3. The fully enclosed corrugated sampler according to claim 2, characterized in that: A limiting clamp (5) is detachably provided on the telescopic part (12), and the limiting clamp (5) restricts the telescopic part (12) and keeps the telescopic part (12) in a retracted state.

4. The fully enclosed corrugated sampler according to claim 2, characterized in that: The end of the telescopic part (12) away from the connecting part (11) is detachably provided with a plug (27), and one end of the sampling strip (3) is fixedly connected to the plug (27).

5. A fully enclosed corrugated sampler according to claim 2, characterized in that: The connecting part (11) is located on the side of the closed section (4) near the interface (2) and has a telescopic tube (30).

6. The fully enclosed corrugated sampler according to claim 1, characterized in that: A guide rod (20) is provided on the side wall of the sleeve (121), and a guide groove (21) is provided on the outer side wall of the upper tube (111) along its own axis. The guide rod (20) is slidably disposed in the guide groove (21).

7. The fully enclosed corrugated sampler according to claim 1, characterized in that: The sleeve (121) is provided with a third elastic element (28), which pushes the guide rod (20) to move toward the upper tube body (111). The top of the upper tube body (111) is provided with a slot (23) that communicates with the guide groove (21).

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