A compound microbial agent for rapid composting of livestock and poultry manure, a preparation method and application thereof

Abstract

The application discloses a kind of for livestock and poultry manure quick rotting complex microbial inoculant and its preparation method and application, belong to microbial technical field.The complex microbial inoculant of the application includes complex microbial fermentation liquor 30-40 parts, organic carrier and conditioner 40-50 parts, nutrient adjuvant 5-8 parts, mineral element containing adjuvant 3-5 parts, accelerator 2-4 parts, wherein complex microbial fermentation liquor includes thermophilic Bacillus, high-temperature-resistant Bacillus heterosporus, nourishing arthrobacter.The three strains of the complex microbial inoculant of the application are selected, and the synergistic effect is remarkable, thermophilic Bacillus and high-temperature-resistant Bacillus heterosporus dominate high-temperature stage, rapidly decompose various organic matters and kill pathogenic bacteria and insect eggs;Nourishing arthrobacter dominates medium-low temperature stage, thoroughly deodorizes and completes humification.Three synergies realize the whole cycle coverage of sheep manure rotting process, and the rotting effect is good, and the rotting material is loose, odorless, and the seed germination index is high.

Description

TECHNICAL FIELD

[0001] The present application belongs to the field of microbial technology, and particularly relates to a compound microbial agent for rapid composting of livestock and poultry manure, a preparation method and application thereof. BACKGROUND

[0002] With the rapid development of large-scale and intensive breeding industry, the amount of livestock and poultry manure is increasing rapidly. If not properly treated, it will not only cause serious environmental pollution such as water eutrophication, air odor, soil compaction, etc., but also lead to the spread of pathogenic bacteria and parasitic eggs, endangering human and animal health. The traditional composting method is one of the main ways to treat livestock and poultry manure, but it has problems such as long fermentation period, large nutrient loss, incomplete composting, heavy odor, etc.

[0003] The traditional composting process of manure relies on natural microorganisms, and has problems such as long fermentation period, slow temperature rise, and heavy odor. Microbial agent technology is an effective way to solve the above problems. By adding exogenous high-efficiency functional microorganisms, the decomposition of organic matter can be accelerated, the composting period can be shortened, the composting quality can be improved, and the odor can be eliminated. There are many related microbial agents in the prior art, but most of them have the shortcomings of single functional strain, poor environmental adaptability, especially insufficient high-temperature resistance, poor compound synergistic effect, etc., resulting in unstable effect in actual application, especially in cold seasons in the north or for the treatment of fresh manure with high water content.

[0004] Therefore, it is of great significance to develop a compound microbial agent with fast temperature rise, strong decomposition ability, significant deodorization effect, and applicability to various environmental conditions, for promoting the resource utilization of livestock and poultry manure. SUMMARY

[0005] The purpose of the present application is to provide a compound microbial agent for rapid composting of livestock and poultry manure, which has strong decomposition ability, significant deodorization effect, and good composting effect.

[0006] To achieve the above technical purpose, the technical scheme adopted by the present application is:

[0007] A compound microbial agent for rapid composting of livestock and poultry manure, comprising the following raw materials by weight: 30-40 parts of compound microbial fermentation liquor, 40-50 parts of organic carrier and conditioner, 5-8 parts of nutritional adjuvant, 3-5 parts of mineral element-containing adjuvant, and 2-4 parts of promoter.

[0008] Further, the complex microbial fermentation liquor comprises Geobacillus thermoleovorans, Aliibacillus thermotolerans and Arthrobacter pascens. The Geobacillus thermoleovorans is purchased from the China Center for Type Culture Collection, with a strain number of CCTCC WB 20081274 and an original preservation date of December 16, 2007. The Aliibacillus thermotolerans is purchased from the China General Microbiological Culture Collection Center, with a strain number of CGMCC 1.15790 and an original preservation date of July 10, 2016. The Arthrobacter pascens is purchased from the China General Microbiological Culture Collection Center, with a strain number of CGMCC 1.6310 and an original preservation date of May 12, 2006.

[0009] Further, the preparation method of the complex microbial fermentation liquor is as follows:

[0010] The Geobacillus thermoleovorans, the Aliibacillus thermotolerans and the Arthrobacter pascens are respectively activated and cultured, and fermentation liquors with a viable bacterial count of 1×10 9 CFU / ml are obtained. The three kinds of fermentation liquors are mixed in a volume ratio of 1:1:1 to obtain the complex microbial fermentation liquor.

[0011] Further, the organic carrier and the conditioner are bentonite and mushroom residue, and the mass ratio of the two is (1-2):1.

[0012] Further, the mushroom residue is sieved through a 20-mesh sieve, and the water content is adjusted to 30-40%.

[0013] Further, the nutrient aid comprises molasses and soybean meal, and the mass ratio of the two is (4-6):1.

[0014] Further, the soybean meal is crushed and sieved through a 60-mesh sieve.

[0015] Further, the mineral element-containing aid is superphosphate.

[0016] Further, the accelerator is humic acid and ferrous sulfate, and the mass ratio of the two is (2-4):1.

[0017] Further, the humic acid is sieved through a 100-mesh sieve.

[0018] A preparation method of a complex microbial agent for rapid composting of livestock and poultry manure, comprising the following steps:

[0019] (1) first prepare the compound microbial fermentation liquor, then dilute the molasses with 50℃ warm water, and then mix the compound microbial fermentation liquor uniformly to form a liquid mixture;

[0020] (2) all of the bentonite, mushroom residue, soybean meal, superphosphate, humic acid and ferrous sulfate are put into a mixer and stirred for 30-45 min, and all dry materials are mixed uniformly;

[0021] (3) under the condition of continuous stirring of the mixer, the liquid mixture prepared in step (1) is uniformly sprayed on the dry materials in step (2), and stirring is continued until the liquid components are uniformly adsorbed by the dry materials, then the mixture is put into a granulator for granulation, and solid particles with a particle size of 3-5 mm are obtained, and low-temperature drying is carried out to obtain the compound microbial inoculant.

[0022] The application also provides the application of the compound microbial inoculant for rapid composting of livestock and poultry manure, which is suitable for treating sheep manure and other livestock and poultry manure.

[0023] The thermophilic bacillus can efficiently degrade cellulose and significantly accelerate the composting process. The thermophilic bacillus can resist extreme high temperature up to 75℃, can quickly decompose macromolecular organic matters such as proteins and starches, and can generate a large amount of heat to rapidly heat the heap.

[0024] The high-temperature-resistant bacillus can assist the thermophilic bacillus to efficiently degrade cellulose, hemicellulose and other difficult-to-decompose organic matters in the high-temperature period, and maintain the decomposition activity in the high-temperature period.

[0025] The trophobacter can also effectively decompose residual organic matter, and has strong deodorizing ability, and can effectively degrade ammonia, hydrogen sulfide and other foul-smelling substances.

[0026] The humic acid and ferrous sulfate added in the application can catalyze and stabilize the formation of humus, the superphosphate can not only adjust the pH, but also can combine the phosphorus element with the nitrogen element to reduce the loss of nitrogen in the form of ammonia, thereby improving the nutrient content and quality of the final composting product, and realizing complete composting and good fertilizer preservation effect.

[0027] The compound microbial inoculant has wide applicability, the functional strains in the inoculant are resistant to high temperature and poor nutrition, and the compounded carrier and conditioner can effectively adjust the air permeability of the manure, so that the inoculant is suitable for treating various livestock and poultry manure such as sheep manure, and can exhibit stable performance under different climate conditions.

[0028] Advantages

[0029] The three selected functional strains of the application have a significant synergistic effect, Geobacillus thermoleovorans and Aliibacillus thermotolerans dominate the high-temperature stage, rapidly decompose various organic matters and kill pathogenic bacteria and insect eggs; Arthrobacter pascens dominates the medium and low-temperature stage, completely deodorizes and completes humification. The three strains cooperate to realize the full-cycle coverage of the composting process, and the efficiency is much higher than that of single bacterial agent or unreasonable bacterial agent, the composting effect is good, the composting material is loose, and the seed germination index is high.

[0030] The compound microbial agent has the advantages of fast temperature rise, long high-temperature stage, effective killing of escherichia coli and insect eggs, greatly reduced risk of pathogen transmission, harmless, effectively shortened composting cycle, and efficient degradation of foul-smelling substances, which fundamentally reduces the generation of ammonia and other foul-smelling gases, and has a significant deodorizing effect. DETAILED DESCRIPTION

[0031] The technical solutions of the application will be further described below in combination with specific embodiments, but are not limited thereto.

[0032] Embodiment 1

[0033] A compound microbial agent for rapid composting of livestock and poultry manure, comprising the following raw materials by weight: 30 parts of compound microbial fermentation liquor, 40 parts of organic carrier and conditioner, 5 parts of nutritional aid, 3 parts of mineral element containing aid, and 2 parts of promoter.

[0034] The compound microbial fermentation liquor comprises Geobacillus thermoleovorans, Aliibacillus thermotolerans and Arthrobacter pascens; the Geobacillus thermoleovorans strain has a strain number of CCTCC WB 20081274; the Aliibacillus thermotolerans strain has a strain number of CGMCC 1.15790; and the Arthrobacter pascens strain has a strain number of CGMCC 1.6310.

[0035] The preparation method of the compound microbial fermentation liquor is as follows:

[0036] The Geobacillus thermoleovorans, Aliibacillus thermotolerans and Arthrobacter pascens are respectively activated and cultured, and 1×10 9 CFU / ml of fermentation liquor is obtained, and the three kinds of fermentation liquor are mixed in a volume ratio of 1:1:1 to obtain the compound microbial fermentation liquor.

[0037] The organic carrier and conditioner are bentonite and mushroom residue, and the mass ratio of the two is 1:1.

[0038] The mushroom residue is sieved through a 20-mesh sieve, and the moisture content is adjusted to 30%.

[0039] The nutrient aid includes molasses and soybean meal, and the mass ratio of the two is 4:1.

[0040] The soybean meal is crushed to pass through a 60-mesh sieve.

[0041] The mineral element-containing aid is superphosphoric acid calcium.

[0042] The accelerator is humic acid and ferrous sulfate, and the mass ratio of the two is 2:1.

[0043] The humic acid is sieved to pass through a 100-mesh sieve.

[0044] A preparation method of a compound microbial agent for rapid composting of livestock and poultry manure, comprising the following steps:

[0045] (1) First, a compound microbial fermentation liquor is prepared, then the molasses is diluted with 50°C warm water, and then the compound microbial fermentation liquor is mixed uniformly to form a liquid mixture;

[0046] (2) Put bentonite, mushroom residue, soybean meal, superphosphoric acid calcium, humic acid and ferrous sulfate into a mixer, stir for 30 min, and mix all the dry materials uniformly;

[0047] (3) Under the condition of continuous stirring of the mixer, uniformly spray the liquid mixture prepared in step (1) on the dry materials in step (2), stir until the liquid components are uniformly adsorbed by the dry materials, put them into a granulator for granulation, obtain solid particles with a particle size of 3-5 mm, and dry at low temperature to obtain the compound microbial agent.

[0048] Example 2

[0049] A compound microbial agent for rapid composting of livestock and poultry manure, comprising the following raw materials by weight: compound microbial fermentation liquor 35 parts, organic carrier and conditioner 45 parts, nutrient aid 7 parts, mineral element-containing aid 4 parts, and accelerator 3 parts.

[0050] The compound microbial fermentation liquor includes Geobacillus thermoleovorans, Aliibacillus thermotolerans and Arthrobacter pascens; the Geobacillus thermoleovorans strain number is CCTCC WB 20081274; the Aliibacillus thermotolerans strain number is CGMCC 1.15790; and the Arthrobacter pascens strain number is CGMCC 1.6310.

[0051] The preparation method of the compound microbial fermentation liquor is as follows:

[0052] The Bacillus thermophilus, Bacillus acidoterrestris and Bacteroides capillosus were activated and expanded respectively, and fermented liquid with 1×10 9 CFU / ml of live bacteria was obtained. The three kinds of fermented liquid were mixed in a volume ratio of 1:1:1 to obtain a composite microbial fermentation liquid.

[0053] The organic carrier and conditioner are bentonite and mushroom residue, and the mass ratio of the two is 2:1.

[0054] The mushroom residue is sieved through a 20-mesh sieve, and the moisture content is adjusted to 35%.

[0055] The nutrient aid includes molasses and soybean meal, and the mass ratio of the two is 5:1.

[0056] The soybean meal is crushed through a 60-mesh sieve.

[0057] The mineral element-containing aid is superphosphate.

[0058] The accelerator is humic acid and ferrous sulfate, and the mass ratio of the two is 3:1.

[0059] The humic acid is sieved through a 100-mesh sieve.

[0060] A preparation method of a composite microbial agent for rapid composting of livestock and poultry manure, comprising the following steps:

[0061] (1) First, prepare a composite microbial fermentation liquid, then dilute the molasses with 50°C warm water, and then mix it evenly with the composite microbial fermentation liquid to form a liquid mixture;

[0062] (2) Put bentonite, mushroom residue, soybean meal, superphosphate, humic acid and ferrous sulfate into a mixer, stir for 35 minutes, and mix all the dry materials evenly;

[0063] (3) Under the condition of continuous stirring of the mixer, evenly spray the liquid mixture prepared in step (1) on the dry materials in step (2), stir until the liquid components are evenly adsorbed by the dry materials, and then put them into a granulator for granulation to obtain solid particles with a particle size of 3-5mm, and then dry at low temperature to obtain a composite microbial agent.

[0064] Example 3

[0065] A composite microbial agent for rapid composting of livestock and poultry manure, comprising the following raw materials by weight: composite microbial fermentation liquid 40 parts, organic carrier and conditioner 50 parts, nutrient aid 8 parts, mineral element-containing aid 5 parts, and accelerator 4 parts.

[0066] The complex microbial fermentation liquor comprises Geobacillus thermoleovorans, Aliibacillus thermotolerans and Arthrobacter pascens; the Geobacillus thermoleovorans strain is numbered as CCTCC WB 20081274; the Aliibacillus thermotolerans strain is numbered as CGMCC 1.15790; and the Arthrobacter pascens strain is numbered as CGMCC 1.6310.

[0067] The preparation method of the complex microbial fermentation liquor is as follows:

[0068] The Geobacillus thermoleovorans, Aliibacillus thermotolerans and Arthrobacter pascens are respectively activated and cultured, and fermentation liquors with a viable bacterial count of 1×10 9 CFU / ml are obtained, and the three kinds of fermentation liquors are mixed in a volume ratio of 1:1:1 to obtain the complex microbial fermentation liquor.

[0069] The organic carrier and the conditioner are bentonite and mushroom residue, and the mass ratio of the two is 1:1.

[0070] The mushroom residue is passed through a 20-mesh sieve, and the moisture content is adjusted to 40%.

[0071] The nutrient aid comprises molasses and soybean meal, and the mass ratio of the two is 6:1.

[0072] The soybean meal is crushed through a 60-mesh sieve.

[0073] The mineral element-containing aid is superphosphate.

[0074] The accelerator is humic acid and ferrous sulfate, and the mass ratio of the two is 4:1.

[0075] The humic acid is passed through a 100-mesh sieve.

[0076] A preparation method of a complex microbial agent for rapid composting of livestock and poultry manure, comprising the following steps:

[0077] (1) first, a complex microbial fermentation liquor is prepared, then molasses is diluted with 50°C warm water, and then the diluted molasses is mixed with the complex microbial fermentation liquor to form a liquid mixture;

[0078] (2) the bentonite, mushroom residue, soybean meal, superphosphate, humic acid and ferrous sulfate are all put into a mixer, stirred for 45 min, and all the dry materials are fully mixed and uniform;

[0079] (3) In the state of continuous stirring of the mixer, the liquid mixture prepared in step (1) is uniformly sprayed on the dry material of step (2), and stirred until the liquid components are uniformly adsorbed by the dry material. Granulation is carried out in a granulator to obtain solid particles with a particle size of 3-5 mm, and low-temperature drying is carried out to obtain a composite microbial agent.

[0080] Comparative Example 1

[0081] In each of the comparative examples, the volume ratio of the fermentation liquid of Bacillus thermophilus, Bacillus acidophilus and Bacteroides capillosus in the preparation of the composite microbial fermentation liquid is changed, and the rest of the raw materials and steps are the same as in Example 1.

[0082] Table 1: Fermentation liquid dosage composition of each comparative example

[0083]

[0084] Performance test

[0085] The test livestock and poultry manure is sheep manure.

[0086] The test has 13 treatment groups: adding the composite microbial agents prepared in Examples 1-3 and Comparative Examples 1-9 and natural composting (blank control). Mix the sheep manure with 0.5% of each treatment group of microbial agent, adjust the moisture content to 55%-60%, and stack in a strip pile for fermentation. Insert a thermometer to record the temperature, and record the appearance of the livestock and poultry manure at 5 days of composting. When the temperature rises above 60°C, turn the pile until the temperature stabilizes and composting is complete.

[0087] After composting is completed, samples are collected using the 5-point mixing sampling method, with about 0.5 kg collected at each point. Organic matter is determined by potassium dichromate volumetric method; total nitrogen is determined by Kjeldahl apparatus; total phosphorus is determined by molybdenum-antimony anti-colorimetric method; total potassium is determined by flame spectrophotometer; and 3 replicates are taken each time during measurement.

[0088] Seed germination index determination: Take 10g of fresh sheep manure from each treatment group after composting is completed, add distilled water at a solid-liquid ratio of 1:10, place each sample in a 30°C, 180rpm shaking incubator for 30min, centrifuge at 6000rpm for 15min, remove 5ml of supernatant and place it in a culture dish lined with two layers of filter paper, then evenly place 20 cabbage seeds in a 25°C incubator for 48h of dark incubation. Distilled water is used as the control group. After 48h, measure the root length and number of seed germination, and calculate the seed germination index according to the following formula:

[0089] Seed germination index = (treatment group seed germination rate x treatment group root length) / (control group seed germination rate x control group root length) x 100%;

[0090] The mortality rate of E. coli and roundworm eggs is determined according to the standard NY / T 525-2021.

[0091] The above results are shown in Tables 2 and 3.

[0092] Table 2 Statistics of microbial inoculants for sheep manure composting in each treatment group

[0093]

[0094] As can be seen from the data in Table 2, after the sheep manure is composted, the organic matter content of Examples 1-3 is low, the composting is more thorough, the properties are stable, and the relative content of nitrogen, phosphorus and potassium is improved to a certain extent. In addition, the seed germination index of Examples 1-3 after composting is as high as 89% or more, indicating that the degree of composting of sheep manure is high and has no toxicity; and the E. coli in the sheep manure composted using the compound microbial inoculants of Examples 1-3 is not detected, and the mortality of roundworm eggs is higher, that is, the safety of the product after composting is also higher.

[0095] Table 3 Appearance and time to reach the highest temperature during the composting process of sheep manure in different treatments

[0096]

[0097] As can be seen from the results in Table 3, the compound microbial inoculants of Examples 1-3 can effectively improve the pile temperature, compost quickly, have no odor, and have the best composting effect. The composting effects of Comparative Examples 1-9, which change the composition of the inoculants, all decrease to varying degrees, especially the composting effect of Comparative Examples 4-9 decreases significantly after the synergistic effect of the three strains disappears.

[0098] It should be noted that the above examples are only part of the preferred modes of implementing the present application, not all. Obviously, based on the above examples of the present application, all other examples obtained by a person of ordinary skill in the art without creative labor should belong to the scope of protection of the present application.

Claims

1. A compound microbial inoculant for the rapid composting of livestock and poultry manure, characterized in that, The raw materials include the following parts by weight: 30-40 parts of compound microbial fermentation broth, 40-50 parts of organic carrier and conditioner, 5-8 parts of nutrient additives, 3-5 parts of mineral element additives, and 2-4 parts of promoters. The composite microbial fermentation broth is composed of *Geobacillus thermoleovorans*, *Aliibacillus thermotolerans*, and *Arthrobacter pascens*. The strain number of *Geobacillus thermoleovorans* is CCTCC WB20081274, the strain number of *Aliibacillus thermotolerans* is CGMCC1.15790, and the strain number of *Arthrobacter pascens* is CGMCC 1.6310. The method for preparing the composite microbial fermentation broth is as follows: Bacillus thermophilus, Heterobacter pyrolyticus, and Arthrotrophic bacteria are activated and cultured separately to obtain viable cell counts of 1×10⁻⁶. 9 The fermentation broth with CFU / ml was mixed with the three fermentation broths in a volume ratio of 1:1:1 to obtain a compound microbial fermentation broth. The organic carrier and conditioner are bentonite and mushroom residue, with a mass ratio of (1-2):1; the nutritional aids include molasses and soybean meal, with a mass ratio of (4-6):1; the mineral element aid is superphosphate; the accelerator is humic acid and ferrous sulfate, with a mass ratio of (2-4):

1.

2. A method for preparing the compound microbial agent for rapid composting of livestock and poultry manure as described in claim 1, characterized in that, Includes the following steps: (1) First, prepare the compound microbial fermentation broth, then dilute the molasses with 50°C warm water, and then mix it evenly with the compound microbial fermentation broth to form a liquid mixture; (2) Put bentonite, mushroom residue, soybean meal, superphosphate, humic acid and ferrous sulfate into the mixer and stir for 30-45 minutes until all dry materials are fully mixed. (3) While the mixer is continuously stirring, the liquid mixture prepared in step (1) is evenly sprayed onto the dry material in step (2), and stirred until the liquid components are evenly adsorbed by the dry material. The mixture is then placed in a granulator for granulation to obtain solid particles with a particle size of 3-5 mm. The mixture is then dried at low temperature to obtain a composite microbial agent. The composite microbial fermentation broth is composed of *Geobacillus thermoleovorans*, *Aliibacillus thermotolerans*, and *Arthrobacter pascens*. The strain number of *Geobacillus thermoleovorans* is CCTCC WB20081274, the strain number of *Aliibacillus thermotolerans* is CGMCC1.15790, and the strain number of *Arthrobacter pascens* is CGMCC 1.6310. The method for preparing the composite microbial fermentation broth is as follows: Bacillus thermophilus, Heterobacter pyrolyticus, and Arthrotrophic bacteria are activated and cultured separately to obtain viable cell counts of 1×10⁻⁶. 9 The three fermentation broths, each containing CFU / ml, were mixed in a volume ratio of 1:1:1 to obtain a composite microbial fermentation broth.

3. The application of the composite microbial agent according to claim 1 in the rapid composting of livestock and poultry manure, characterized in that, The livestock and poultry manure mentioned is sheep manure.

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