Anchor cell construct culture

By using anchors to reshape cell sheets in a cell culture device, the problem of uncontrolled folding of cell sheets without anchors was solved, resulting in stable 3D cell constructs suitable for in vitro modeling and in vivo applications.

CN121152869APending Publication Date: 2025-12-16CARO MEATS INC
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Patent Information

Application Number
CN202480029511.8
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Priority Date
2023-10-17
Filing Date
2024-05-29
Publication Date
2025-12-16

AI Technical Summary

Technical Problem

In existing technologies, cell sheets tend to fold and aggregate uncontrollably when cultured without anchors, resulting in reduced cell viability, making it difficult to accurately represent cells in vivo. Furthermore, the difficulty in handling these sheets limits their applications in in vitro modeling and drug discovery.

Method used

Using a cell culture device, multiple anchors are placed on the membrane, allowing cells to attach and remodel into 3D cell constructs. The anchors are located within the membrane perimeter, promoting cell sheets to detach from the membrane and remodel into stable constructs.

Benefits of technology

It achieves stable remodeling of cell sheets, forming 3D constructs that can accurately represent cells in vivo, suitable for in vitro modeling and in vivo applications, improving cell viability and ease of operation.

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Abstract

The scaffolding-free cell constructs are prepared from cell sheet layers. The device has a membrane and an anchor located within a perimeter or within a wall of the membrane. The membrane allows cell attachment and subsequent detachment of the cells and secreted ECM as a cell sheet. In the method, the cells grow into a cell lamella. The sheet of cells is located around the two or more anchors. The sheet of cells is remodeled into cell constructs of different shapes that attach to the anchor. The anchor forms and passes through the opening in the cell sheet as the cell sheet grows in the device. The detachment of the cell sheet causes the cell sheet to be positioned around the anchor. The cell constructs can be used either in decellularized or non-decellularized conditions, and when attached to an anchor, e.g., as an in vitro model, or after removal from the anchor, e.g., for regenerative medicine or tissue engineering.
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Citation Information

Patent Citations

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    US11718830B1

  • Heart muscle-like cell sheet, three-dimensional construct, heart muscle-like tissue and process for producing the same

    US20040009566A1

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