Traditional Chinese medicinal material protoplast breeding equipment and breeding method thereof

Abstract

The invention discloses traditional Chinese medicinal material protoplast breeding equipment and a breeding method thereof, and belongs to the technical field of breeding equipment.The traditional Chinese medicinal material protoplast breeding equipment comprises a cultivation box, a breeding device, a breeding device and a control device, a partition plate is fixedly installed in the cultivation box, and a tray is arranged above the partition plate; the drainage device is arranged on one side of the cultivation box, the water inlet end is communicated with the space above the partition plate, and the water outlet end of the drainage device is located below the partition plate; the humidifying box is fixedly installed on the side, away from the drainage device, of the cultivation box, a water outlet is formed between the bottom of the humidifying box and the cultivation box, in the early stage, protoplast is directly soaked, cell division and growth are promoted, in the later stage, the contact between traditional Chinese medicine protoplast and oxygen is increased, and metabolic activity and morphogenesis are facilitated; part of the nutrient solution is converted into fine water mist, on one hand, the air humidity is increased, on the other hand, trace nutrients are carried and attached to the surface of the traditional Chinese medicine protoplast, continuous nutrition supply is provided for the traditional Chinese medicine protoplast, and cyclic utilization of the nutrient solution is achieved.

Description

Technical Field

[0001] This invention relates to the field of breeding equipment technology, and in particular to a protoplasmic breeding device for Chinese medicinal materials and its breeding method. Background Technology

[0002] As an important part of my country's traditional medicine, Chinese medicinal materials play an irreplaceable role in modern biomedicine and the health industry. With the development of plant tissue culture technology, the isolation and culture of protoplasts of Chinese medicinal materials has become an important means to realize the genetic improvement of medicinal plants, genetic engineering research and the production of secondary metabolites. Protoplasts refer to the living plant cells after the cell walls have been removed. They have high totipotency and can regenerate complete plants under suitable conditions. Therefore, they have broad application prospects in medicinal plant breeding, stress resistance improvement and molecular biology research.

[0003] Currently, the culture of protoplasts of Chinese medicinal materials mostly adopts traditional liquid suspension culture or solid plate culture methods, which rely on manual operation to control environmental conditions, such as light, temperature, humidity, and nutrient supply.

[0004] However, traditional culture systems are mostly static liquid environments, which make it difficult to achieve a periodic oxygen supply mode, which is not conducive to gas exchange and metabolic balance of protoplasts and limits their growth vitality. Summary of the Invention

[0005] The purpose of this invention is to address the problem that existing cultivation systems are mostly static liquid environments, which make it difficult to achieve a periodic oxygen supply mode, hindering gas exchange and metabolic balance of protoplasts and limiting their growth vitality. Therefore, this invention proposes a protoplast breeding device and breeding method for Chinese medicinal materials.

[0006] To achieve the above objectives, the present invention adopts the following technical solution: A protoplasmic breeding device for Chinese medicinal materials includes: a cultivation box with a partition fixedly installed inside, and a tray above the partition; a drainage device located on one side of the cultivation box, with the inlet of the drainage device communicating with the space above the partition and the outlet of the drainage device located below the partition, wherein the drainage device automatically discharges nutrient solution when the liquid level in the cultivation box reaches a set value; and a humidification box fixedly installed on the side of the cultivation box away from the drainage device, with an outlet between the bottom of the humidification box and the cultivation box for receiving the nutrient solution discharged by the drainage device.

[0007] To avoid root rot or metabolic inhibition caused by oxygen deficiency in traditional hydroponics through tidal hydroponics and to simulate a growth rhythm closer to nature, the drainage device preferably includes a first L-shaped baffle fixedly installed to the edge of the cultivation box, and further includes: a bent plate and a first separation plate fixedly installed on the partition, with an outlet on the partition between the bent plate and the first separation plate; a first hook-shaped plate located above the bent plate, with a flow channel between the two plates; a second hook-shaped plate located above the first hook-shaped plate, with its vertical edge collinear with the first separation plate and a flow channel between them; a second separation plate having an arc-shaped portion fixedly connected to the second hook-shaped plate, located between the first separation plate and the side wall of the cultivation box; and a second L-shaped baffle fixed to the bent plate, located between the first L-shaped baffle and the second hook-shaped plate, with a through hole on the second L-shaped baffle.

[0008] To mitigate water flow impact, improve liquid flow path, and prevent turbulence, a first arc-shaped block is further fixedly installed between the first L-shaped baffle and the side wall of the incubator.

[0009] To smooth the liquid transition and improve drainage, a second arc-shaped block is further fixedly installed between the first separation plate and the side wall of the incubator.

[0010] To kill bacteria, fungi, and viruses in the nutrient solution, a guide plate is fixedly installed at one end of the bent plate and the first separation plate below the partition. A first water wheel is installed in the incubation tank below the partition, located below the guide plate. A support rod is fixedly installed on the bottom surface of the incubation tank, and a hollow shaft is rotatably installed inside the support rod. A push rod is fixedly installed at one end of the hollow shaft. A piston that abuts against the push rod is fixedly installed on the top surface of the partition. One end of the piston is connected to an air source, and a hose is fixedly installed at the other end of the piston. The hose is rotatably connected to the hollow shaft, and a turntable is fixedly installed at the other end of the hollow shaft. Inclined grooves are formed on the opposite surfaces of the turntable and the first water wheel. A spring is sleeved on the hollow shaft, and both ends of the spring are fixedly connected to the support rod.

[0011] To ensure uniform gas release, flexible belts are further installed at equal intervals on the hollow shaft, and air holes are provided on the flexible belts.

[0012] To further facilitate the entry of atomized water vapor into the cultivation environment, the humidification chamber includes a chamber body with a flow outlet, and further includes: an upper water tank and a lower water tank installed inside the chamber body, with a water curtain stretched between the upper and lower water tanks; a drain pipe fixedly installed on the lower water tank, with a pump body installed on the drain pipe; a return water pipe fixedly installed inside the chamber body, with a pump body installed on it, one end of the return water pipe fixed to the upper water tank, and the other end extending below the lower water tank; and a fan fixedly installed inside the chamber body, with its air outlet facing the water curtain.

[0013] To further achieve self-cleaning of the filter screen, a collection bucket and a second water wheel are rotatably installed inside the water tank. A rotating wheel is installed at the bottom of the box. The drive shafts of the second water wheel and the rotating wheel both pass through the box and are connected by belt drive. The hollow shaft is slidably connected to the rotating wheel. The hollow shaft has a crank portion, and a pull rod is rotatably installed on the crank portion. A brush plate is slidably installed on the water outlet, and the brush plate is rotatably connected to the pull rod.

[0014] To facilitate the addition of trace elements, growth regulators, disinfectants, etc. to the nutrient solution, the box is further equipped with a feeding port located above the lower water tank.

[0015] A method for protoplasmic breeding of Chinese medicinal materials includes the following steps: Step 1: Prepare a suitable nutrient solution for the growth of the medicinal herb protoplasts according to their requirements, and add it to the cultivation container; Step 2: Inoculate the pretreated protoplasts of Chinese medicinal materials into the culture container; Step 3: When the nutrient solution reaches the set height, the nutrient solution in the culture container can be automatically discharged. Then, the discharged nutrient solution is atomized and sprayed back into the culture container, which increases the air humidity and provides the necessary water and nutrients for the protoplasts.

[0016] Compared with the prior art, the present invention provides a device for protoplasmic breeding of Chinese medicinal materials, which has the following beneficial effects: 1. In the initial stage of this herbal protoplast breeding equipment, nutrient solution is injected into the upper space of the incubation box, and the herbal protoplasts are placed on the tray in a direct immersion state, which promotes early cell division and growth. When the nutrient solution decreases due to evaporation, it can be replenished through the humidification box. When the liquid level rises above the set threshold, the drainage device is automatically activated to drain the excess liquid, increasing the contact between the herbal protoplasts and oxygen, which is beneficial to metabolic activities and morphogenesis. The drained nutrient solution flows into the humidification box through the outlet. The humidification box converts part of the nutrient solution into fine water mist, which is then sent into the incubation box. The water mist increases the air humidity and carries trace nutrients to the surface of the herbal protoplasts, providing them with a continuous supply of nutrients. This realizes the recycling of nutrient solution, avoids the herbal protoplasts from being exposed to a dry environment for a long time, reduces dehydration damage, and improves the survival rate. 2. This protoplasmic breeding equipment for Chinese medicinal materials collects the nutrient solution flowing through the water curtain into a collection tank. After a period of collection, the solution is flushed onto the second water wheel, causing it to rotate. Power is transmitted to the impeller via a belt, which in turn drives the hollow shaft to rotate. The crank on the hollow shaft drives the pull rod to move. The pull rod pushes the brush plate to slide back and forth along the direction of the water outlet, realizing an automatic cleaning function and preventing the filter screen from clogging. At the same time, the first water wheel rotates along with it, throwing out its own residual nutrient solution to help rinse the filter screen and achieve self-cleaning. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the overall structure of a protoplasmic breeding device for Chinese medicinal materials proposed in this invention; Figure 2 This is a schematic diagram of the internal structure of a protoplasmic breeding device for Chinese medicinal materials proposed in this invention; Figure 3 for Figure 2 Second perspective; Figure 4 This is a schematic diagram of the tray structure of a protoplasmic breeding device for Chinese medicinal materials proposed in this invention; Figure 5 This is a schematic diagram of the hollow shaft structure of a protoplasmic breeding device for Chinese medicinal materials proposed in this invention; Figure 6 This is a schematic plan view of the drainage device of a protoplasmic breeding equipment for Chinese medicinal materials proposed in this invention; Figure 7 This is a schematic diagram of the internal gas-liquid distribution during use of the drainage device of the protoplasm breeding equipment for Chinese medicinal materials proposed in this invention; Figure 8 This is a schematic diagram of the internal structure of the humidification chamber of a protoplasm breeding device for Chinese medicinal materials proposed in this invention; Figure 9 This invention proposes a protoplasmic breeding device for Chinese medicinal materials. Figure 5Enlarged view of the A-structure.

[0018] In the diagram: 100, incubator; 110, partition; 111, liquid outlet; 120, tray; 121, grid frame; 122, petri dish; 130, first water wheel; 140, support rod; 150, hollow shaft; 151, push rod; 152, turntable; 153, spring; 154, flexible belt; 155, crank; 156, pull rod; 157, brush plate; 160, piston; 161, hose; 101, inclined groove; 102, vent. 200. Drainage device; 210. First L-shaped baffle; 211. First arc-shaped block; 220. Bending plate; 230. First separation plate; 231. Second arc-shaped block; 240. First hook-shaped plate; 250. Second hook-shaped plate; 260. Second separation plate; 261. Arc-shaped part; 270. Second L-shaped baffle; 271. Through hole; 280. Guide plate; 290. Back plate; 300. Humidification chamber; 310. Chamber body; 311. Flow port; 312. Rotary wheel; 313. Feed port; 320. Upper water tank; 330. Lower water tank; 331. Collection bucket; 332. Second water wheel; 333. Drain pipe; 340. Water curtain; 350. Return water pipe; 360. Fan; 301. Water outlet; 302. Filter screen. Detailed Implementation

[0019] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments.

[0020] In the description of this invention, it should be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this invention. Example

[0021] Reference Figures 1-9 A protoplasmic breeding device for Chinese medicinal materials includes a cultivation box 100, a drainage device 200, and a humidification box 300. The components work together to form a closed breeding system with automatic nutrient solution regulation and recycling functions.

[0022] Specifically, the cultivation box 100 is used to contain the protoplasts of Chinese medicinal materials and the nutrient solution required for their cultivation. A partition 110 is fixedly installed inside, dividing the space of the cultivation box 100 into upper and lower parts. A tray 120 is set above the partition 110 to support the protoplasts of Chinese medicinal materials. Specifically, the tray 120 includes a detachable grid frame 121 and a culture dish 122. The culture dish 122 is embedded in the grid frame 121 to facilitate ventilation and prevent accumulation, thus realizing flexible switching between hydroponics and aeroponics.

[0023] The drainage device 200 is located on one side of the incubator 100. The inlet of the drainage device 200 is connected to the space above the partition 110. It is used to detect and control the liquid level of the nutrient solution in the incubator 100. The outlet of the drainage device 200 is located below the partition 110. When the liquid level in the incubator 100 reaches the set value, the drainage device 200 automatically discharges the nutrient solution.

[0024] The humidification box 300 is fixedly installed on the side of the incubator 100 away from the drainage device 200. A water outlet 301 is provided between the bottom of the humidification box 300 and the incubator 100. Preferably, a filter screen 302 is installed in the water outlet 301 to intercept suspended particles and impurities, prevent clogging of the subsequent atomizing device, and receive the nutrient solution discharged by the drainage device 200.

[0025] This invention provides a protoplast breeding device for Chinese medicinal materials. In the initial stage, nutrient solution is injected into the upper space of the cultivation tank 100, and the protoplasts of Chinese medicinal materials are placed on the tray 120 in a direct immersion state to promote early cell division and growth. When the nutrient solution decreases due to evaporation, it can be replenished through the humidification tank 300. When the liquid level rises above a set threshold, the drainage device 200 is automatically activated to drain the excess liquid, increasing the contact between the protoplasts of Chinese medicinal materials and oxygen, which is beneficial to metabolic activities and morphogenesis. The drained nutrient solution flows into the humidification tank 300 through the outlet 301. The humidification tank 300 converts part of the nutrient solution into fine water mist, which is then sent into the cultivation tank 100. The water mist increases the air humidity and carries trace nutrients to adhere to the surface of the protoplasts of Chinese medicinal materials, providing them with a continuous supply of nutrients. This realizes the recycling of nutrient solution, avoids the protoplasts of Chinese medicinal materials being exposed to a dry environment for a long time, reduces dehydration damage, and improves the survival rate.

[0026] Reference Figures 2-3 and Figures 7-8 The drainage device 200 includes a first L-shaped baffle 210, which is fixedly installed on the edge of the incubator 100. Further optimization is achieved by fixing a first arc-shaped block 211 between the first L-shaped baffle 210 and the side wall of the incubator 100 to mitigate water flow impact, improve the liquid flow path, and prevent turbulence.

[0027] It also includes a bent plate 220 and a first separation plate 230 fixedly installed on the partition 110. Further optimized, a second arc-shaped block 231 is fixedly installed between the first separation plate 230 and the side wall of the incubator 100 to smooth liquid transition and improve drainage. An outlet 111 is provided on the partition 110 between the bent plate 220 and the first separation plate 230 for discharging nutrient solution. A first hook-shaped plate 240 is located above the bent plate 220, and a flow channel is provided between it and the bent plate 220. A second hook-shaped plate 250 is located above the first hook-shaped plate 240. The vertical edge of plate 250 is collinear with the first separation plate 230, and a flow channel is provided between them. The second hook-shaped plate 250 serves as a secondary buffer structure to further regulate the liquid flow. The second separation plate 260 has an arc-shaped portion 261, which is fixedly connected to the second hook-shaped plate 250 and located between the first separation plate 230 and the side wall of the incubator 100, for separating liquid and air. The second L-shaped baffle 270 is fixed to the bending plate 220 and located between the first L-shaped baffle 210 and the second hook-shaped plate 250. The second L-shaped baffle 270 has a through hole 271.

[0028] It should be noted that a back plate 290 is installed between the second L-shaped baffle 270 and the second hook-shaped plate 250 to enclose part of the space, forming a partially sealed chamber, which provides a basis for siphon start-up.

[0029] With the above-described structure, as the nutrient solution is injected into the upper space of the incubator 100, the liquid level slowly rises, passing sequentially through the first L-shaped baffle 210, the bending plate 220, and other guiding structures. At this time, drainage is not initiated, and the system is in a static state. When the water surface contacts the second L-shaped baffle 270, it enters the space formed between the second L-shaped baffle 270 and the bending plate 220 through the through hole 271. As the liquid level continues to rise, the liquid passes over the apex of the second hook-shaped plate 250 and flows into the inner chamber space formed between the back plate 290 and the second L-shaped baffle 270. When the liquid level rises further and passes over the apex of the first hook-shaped plate 240, a first-stage siphon effect is triggered. Because the aperture of the through hole 271 is small, it cannot meet the flow rate of the first-stage siphon, and the water in the inner chamber quickly... The liquid will be sucked dry, thus opening the connection between the outer chamber and the air. After the inner chamber is emptied, a significant liquid level difference appears between the main cultivation area and the inner chamber. The liquid level in the outer chamber will drop instantly, triggering a secondary siphon. Since the through hole 271 is the only air inlet and outlet, the inner chamber maintains a negative pressure state, promoting continuous drainage. The discharged nutrient solution flows into the lower space or humidification box 300 through the liquid outlet 111. As the liquid level drops below the top of the first hook plate 240, the siphon effect disappears, and the system returns to its initial state, waiting for the next liquid level rise before starting drainage again. Through this tidal hydroponics, the root rot or metabolic inhibition problems caused by lack of oxygen in traditional hydroponics are avoided. It can simulate a growth rhythm closer to nature, which is beneficial to cell division and tissue regeneration.

[0030] Reference Figures 2-3 and Figure 5 and Figure 9 A guide plate 280 is fixedly installed at one end of the bending plate 220 and the first separation plate 230 below the partition plate 110. A first water wheel 130 is installed inside the incubation box 100 below the partition plate 110, and the first water wheel 130 is located below the guide plate 280. A support rod 140 is fixedly installed on the bottom surface inside the incubation box 100, and a hollow shaft 150 is rotatably installed inside the support rod 140. Preferably, flexible belts 154 are equidistantly installed on the hollow shaft 150, and air holes 102 are opened on the flexible belts 154. A push rod 151 is fixedly installed at one end of the hollow shaft 150, and a piston that abuts against the push rod 151 is fixedly installed on the top surface inside the partition plate 110. The piston component 160 has an external air source connected to one end, and a hose 161 fixedly installed at the other end. The hose 161 is rotatably connected to the hollow shaft 150 and is used to transmit air pressure to the interior of the hollow shaft 150. Preferably, the air source is an ozone generator. When squeezed by the push rod 151, ozone is forced into the hose 161 to achieve timed release of ozone. A turntable 152 is fixedly installed at the other end of the hollow shaft 150. Inclined grooves 101 are opened on the opposite surfaces of the turntable 152 and the first water wheel 130. A spring 153 is sleeved on the hollow shaft 150, and the two ends of the spring 153 are fixedly connected to the support rod 140.

[0031] With the above structure, when the nutrient solution level reaches the set height, the drainage device 200 is activated. The liquid is guided by the guide plate 280 and impacts the first water wheel 130, causing it to rotate. The inclined groove 101 on the first water wheel 130 abuts against the inclined groove 101 on the turntable 152, changing the original rotational motion into the linear motion of the hollow shaft 150. The push rod 151 at the front end of the hollow shaft 150 moves forward and squeezes the piston 160. After being compressed, the piston 160 delivers the ozone gas stored inside through the hose 161 to the hollow shaft 150. The ozone enters the flexible belt 154 along the hollow shaft 150 and is finally released into the nutrient solution through the pores 102 on the flexible belt 154. This can effectively kill bacteria, fungi, and viruses in the nutrient solution, while dissolving some ozone to generate oxygen, increasing the dissolved oxygen content of the nutrient solution, which helps the protoplast roots of Chinese medicinal materials grow healthily and reduces the risk of disease.

[0032] Reference Figures 2-3 and Figure 5 and Figure 8The humidification chamber 300 includes a chamber body 310 with a flow port 311 to allow atomized water vapor to enter the cultivation environment. It also includes an upper water tank 320 and a lower water tank 330 installed inside the chamber body 310, with a water curtain 340 tensioned between them. Further optimized, a drain pipe 333 is fixedly installed on the lower water tank 330, and a pump is installed on the drain pipe 333 for discharging liquid or transporting it to other processing units; a return water pipe 350 is fixedly installed inside the chamber body 310, with a pump installed on it; one end of the return water pipe 350 is fixed to the upper water tank 320, and the other end extends below the lower water tank 330; and a fan 360 is fixedly installed inside the chamber body 310, with its outlet facing the water curtain 340.

[0033] With the above-described structure, the nutrient solution discharged from the drainage device 200 flows into the humidification box 300. The liquid first enters the space below the lower water tank 330. The pump starts and transports the liquid to the upper water tank 320 through the return water pipe 350. The liquid in the upper water tank 320 slowly flows down and wets the water curtain 340. The water curtain 340 remains continuously moist, providing a basis for subsequent evaporation. The fan 360 starts and blows air onto the surface of the water curtain 340. The air absorbs moisture as it passes through the moist water curtain 340, forming high-humidity air. The humid air is sent into the cultivation box 100 through the flow port 311 to regulate its internal humidity environment. The liquid flowing through the water curtain 340 finally falls into the lower water tank 330 for storage, and then is uniformly discharged back into the cultivation box 100, forming a tidal hydroponics system, which provides a dynamic, healthy, and controllable growth environment for the protoplasts of Chinese medicinal materials.

[0034] Reference Figure 8 A collection bucket 331 and a second water wheel 332 are rotatably installed inside the water tank 330. In this design, the collection bucket 331 is conical and is used to collect nutrient solution from the water curtain 340. When the collection bucket 331 is filled with nutrient solution, its center of gravity gradually shifts towards the water injection side. When the water volume reaches 2 / 3 of the total volume, the center of gravity exceeds the vertical line of the rotating shaft, and the collection bucket 331 quickly flips around the rotating shaft to empty. A rotating wheel 312 is installed at the bottom of the tank 310. The drive shafts of the second water wheel 332 and the rotating wheel 312 both pass through the tank 310 and are connected by belt drive. The hollow shaft 150 is slidably connected to the rotating wheel 312. The hollow shaft 150 has a crank part 155, and a pull rod 156 is rotatably installed on the crank part 155. A brush plate 157 is slidably installed on the water outlet 301, and the brush plate 157 is rotatably connected to the pull rod 156.

[0035] Furthermore, the tank 310 is equipped with a feeding port 313 for adding trace elements, growth regulators, disinfectants, etc. to the nutrient solution. The feeding port 313 is located above the water tank 330 and is particularly suitable for replenishing beneficial components or stabilizing pH value after ozone treatment.

[0036] With the above structure, the nutrient solution flowing through the water curtain 340 is collected in the collection tank 331. After a period of collection, it is flushed towards the second water wheel 332 to make it rotate. The power is transmitted to the rotating wheel 312 through the belt, which in turn drives the hollow shaft 150 to rotate. The crank part 155 on the hollow shaft 150 drives the pull rod 156 to move. The pull rod 156 pushes the brush plate 157 to slide back and forth along the direction of the water outlet 301 to realize the automatic cleaning function and prevent the filter screen 302 from becoming clogged. At the same time, the first water wheel 130 rotates along with it, throwing out its own residual nutrient solution to help wash the filter screen 302. Trace elements or regulators are added to the circulating nutrient solution through the feeding port 313 to realize dynamic management of the nutrient solution quality.

[0037] A method for protoplasmic breeding of Chinese medicinal materials includes the following steps: Step 1: Inoculate the protoplast suspension with good activity into the culture dish 122 in the tray 120, ensuring that the protoplasts are evenly distributed and avoiding aggregation; Step 2: In the initial stage, nutrient solution is injected into the upper space of the incubator 100 to directly immerse the protoplasts. The light intensity is controlled at 1000-3000 lx, the photocycle is 16 h light / 8 h dark, the temperature is controlled at 20-25℃, and the air humidity is maintained above 70%. Step 3: Through dynamic regulation of nutrient solution and tidal culture, the nutrient solution is maintained at a set height under the rising tide mechanism, and the protoplasts absorb water and nutrients. Under the receding tide mechanism, the drainage device 200 is activated to discharge excess liquid, exposing the protoplasts to the air and enhancing oxygen supply. The discharged nutrient solution is guided by the guide plate 280 to impact the first water wheel 130. The rotation of the first water wheel 130 pushes the hollow shaft 150 to trigger the piston 160. Ozone gas enters the hollow shaft 150 through the hose 161 and is released through the pores 102 on the flexible belt 154, thereby achieving sterilization, oxygenation, and inhibition of microbial contamination of the nutrient solution. Step 4: The discharged nutrient solution flows into the humidification box 300 and is pumped to the upper water tank 320 by the pump body. The liquid flows slowly down the water curtain 340, and the fan 360 blows air to accelerate evaporation. The high humidity air is sent into the incubator 100 through the flow port 311 to maintain suitable growth humidity. Step 5: The nutrient solution flowing through the water curtain 340 is collected in the collection tank 331. After a period of collection, it is flushed onto the second water wheel 332 to make it rotate. The power is transmitted to the rotating wheel 312 through the belt, which in turn drives the hollow shaft 150 to rotate. The crank part 155 on the hollow shaft 150 drives the pull rod 156 to move. The pull rod 156 pushes the brush plate 157 to slide back and forth along the direction of the water outlet 301 to realize the automatic cleaning function and prevent the filter screen 302 from being blocked. At the same time, the first water wheel 130 rotates along with it, throwing out its own residual nutrient solution to help wash the filter screen 302. Step 6: Add trace elements or regulators to the circulating nutrient solution through feed port 313 to achieve dynamic management of nutrient solution quality.

[0038] The above description is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.

Claims

1. A traditional Chinese medicinal material protoplast breeding device, characterized in that, The utility model relates to a kind of cultivation box and its drainage device, including: Cultivation box (100), inside fixed mounting has baffle (110), tray (120) is provided above the baffle (110); Drainage device (200), it is provided in one side of the cultivation box (100), the water inlet end of the drainage device (200) is communicated with the space above the baffle (110), the water outlet end of the drainage device (200) is located below the baffle (110), when the liquid level height in the cultivation box (100) reaches set value, the drainage device (200) automatically drains nutrient solution; Humidification box (300), fixed mounting is in the side of the cultivation box (100) away from the drainage device (200), water outlet (301) is provided between the bottom of the humidification box (300) and the cultivation box (100), for receiving the nutrient solution drained by the drainage device (200).

2. The traditional Chinese medicinal material protoplast breeding device according to claim 1, characterized in that, The drainage device (200) includes first L-shaped baffle (210), and fixed mounting is with the edge of the cultivation box (100), further including: Bent plate (220) and first separation plate (230) fixedly installed on the baffle (110), liquid outlet (111) is opened on the baffle (110) between the bent plate (220) and first separation plate (230); First hook-shaped plate (240), it is located above the bent plate (220), and flow-through passage is provided between the bent plate (220) and the first hook-shaped plate (240); Second hook-shaped plate (250), it is located above the first hook-shaped plate (240), and the vertical edge of the second hook-shaped plate (250) is collinear with the first separation plate (230), and flow-through passage is provided between the first separation plate (230) and the second hook-shaped plate (250); Second separation plate (260), it has arc portion (261), and is fixedly connected with the second hook-shaped plate (250), and is located between the first separation plate (230) and the side wall of the cultivation box (100); Second L-shaped baffle (270), it is fixed with the bent plate (220), and is located between the first L-shaped baffle (210) and the second hook-shaped plate (250), and through-hole (271) is opened on the second L-shaped baffle (270).

3. The traditional Chinese medicinal material protoplast breeding device according to claim 2, characterized in that, First arc-shaped block (211) is fixedly installed between the first L-shaped baffle (210) and the side wall of the cultivation box (100).

4. The traditional Chinese medicinal material protoplast breeding device according to claim 2, characterized in that, Second arc-shaped block (231) is fixedly installed between the first separation plate (230) and the side wall of the cultivation box (100).

5. The traditional Chinese medicinal material protoplast breeding device according to claim 2, characterized in that, Baffle (280) is fixedly installed at one end of the bent plate (220) and the first separation plate (230) below the baffle (110), first water wheel (130) is installed in the cultivation box (100) below the baffle (110), the first water wheel (130) is located below the baffle (280), The bottom surface of the incubator (100) is fixedly provided with a support rod (140), the support rod (140) is rotatably provided with a hollow shaft (150), one end of the hollow shaft (150) is fixedly provided with a push rod (151), the top surface of the partition plate (110) is fixedly provided with a piston (160) abutting against the push rod (151), one end of the piston (160) is connected with an air source, the other end of the piston (160) is fixedly provided with a hose (161), the hose (161) is rotatably connected with the hollow shaft (150), the other end of the hollow shaft (150) is fixedly provided with a rotating disc (152), the rotating disc (152) and the opposite surface of the first water wheel (130) are both provided with an inclined groove (101). The hollow shaft (150) is sleeved with a spring (153), and the two ends of the spring (153) are fixedly connected with the support rod (140).

6. The traditional Chinese medicinal material protoplast breeding device according to claim 5, characterized in that, The hollow shaft (150) is equidistantly provided with a flexible belt (154), and the flexible belt (154) is provided with an air hole (102).

7. The traditional Chinese medicinal material protoplast breeding device according to claim 5, characterized in that, The humidifying box (300) comprises a box body (310), the box body (310) is provided with a flow-through opening (311), and further comprises: An upper water tank (320) and a lower water tank (330) are installed in the box body (310), and a water curtain (340) is tightly arranged between the upper water tank (320) and the lower water tank (330), The lower water tank (330) is fixedly provided with a drain pipe (333), and the drain pipe (333) is provided with a pump body; A return water pipe (350) is fixedly installed in the box body (310) and is provided with a pump body, one end of the return water pipe (350) is fixedly connected with the upper water tank (320), and the other end of the return water pipe (350) extends below the lower water tank (330); A fan (360) is fixedly installed in the box body (310), and the air outlet end of the fan (360) faces the water curtain (340).

8. The traditional Chinese medicinal material protoplast breeding device according to claim 7, characterized in that, The lower water tank (330) is rotatably provided with a collecting barrel (331) and a second water wheel (332), the box body (310) is provided with a rotating wheel (312), the transmission shafts of the second water wheel (332) and the rotating wheel (312) both penetrate out of the box body (310) and are connected through a belt drive, The hollow shaft (150) is slidably connected with the rotating wheel (312), the hollow shaft (150) has a crank portion (155), the crank portion (155) is rotatably provided with a pull rod (156), the water outlet (301) is slidably provided with a brush plate (157), and the brush plate (157) is rotatably connected with the pull rod (156).

9. The traditional Chinese medicinal material protoplast breeding device according to claim 8, characterized in that, The box body (310) is provided with a feeding opening (313), and the feeding opening (313) is located above the lower water tank (330).

10. A method for protoplast breeding of Chinese herbal medicine, using the protoplast breeding equipment according to any one of claims 1-9, characterized in that, The method comprises the following steps: Step one: according to the demand of the Chinese herbal medicine protoplast, a nutrient solution suitable for growth is prepared and added into the incubation container; Step two: the pretreated Chinese herbal medicine protoplast is inoculated into the incubation container; Step three: when the nutrient solution reaches the set height, the nutrient solution in the cultivation container can be automatically discharged, and then the discharged nutrient solution is atomized and sprayed into the cultivation container again, which increases the air humidity and also provides the protoplasts with the necessary moisture and nutrients.

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