Selenium-rich zinc-rich functional feed additive for hyline brown laying hens and preparation method of selenium-rich zinc-rich functional feed additive

Abstract

The invention relates to the technical field of feed additives for laying hens, in particular to a functional feed additive for selenium-rich and zinc-rich hyline brown laying hens and a preparation method of the functional feed additive. The feed additive is prepared from the following raw materials: selenium yeast, zinc methionine, borneol, quince extract, rhizoma cibotii extract, cistanche deserticola extract, angelica sinensis extract, valeriana jatamansi jones-fennel acorus calamus fermentation extract, radix fici simplicissimae oligosaccharide extract and liquorice extract. The inner core of the additive core-shell structure particle comprises quince extract, borneol and selenium yeast; the outer layer of each core-shell structure particle comprises zinc methionine, a rhizoma cibotii extract, a cistanche deserticola extract, an angelica sinensis extract, a valeriana jatamansi-fennel acorus calamus fermentation extract, a radix fici simplicissimae oligosaccharide extract and a liquorice root extract, the inner core wraps borneol molecules in a micro-emulsion form of a quince extract, and selenium yeast is dispersed in micro-emulsion gaps; and the borneol and the selenium yeast are slowly released in the alkaline environment of the intestinal tract, so that the selenium-zinc absorption time sequence separation is realized.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of laying hen feed additives, in particular to a functional feed additive for selenium and zinc-enriched Hailan brown laying hens and a preparation method thereof. BACKGROUND

[0002] Since 1985, China's total poultry egg production has ranked first in the world, and the per capita poultry egg ownership has reached the level of developed countries. With the improvement of people's living standards, the demand for poultry egg quality and food safety will continue to increase. In the production process, particular attention should be paid to the use of prohibited drugs and drug residues in laying hen feed. In recent years, China and the world have increasingly recognized the problem of antibiotic drug residues in poultry. Since July 1, 2020, feed production enterprises have stopped producing commercial feed containing growth-promoting drug feed additives (except for traditional Chinese medicine).

[0003] In the prior art, although pure traditional Chinese medicine preparations are used in additives to prepare a laying hen feed additive that is non-toxic, non-antibiotic, and non-residual, in order to achieve the synergistic effect of anti-inflammatory and absorption promotion and trace element strengthening, ice and organic selenium and zinc methionine are introduced into the additive. However, in actual application, the complex precipitate and intestinal absorption antagonism generated by the direct mixing of ice and selenium and zinc ions in the feed will cause ice and zinc ions to form insoluble precipitates, resulting in the loss of anti-inflammatory and absorption promotion functions, and the competition for absorption carriers in the intestinal tract between selenium and zinc, which can easily cause thinning of the eggshell and decline of the immune function of laying hens. SUMMARY

[0004] The present application provides a functional feed additive for selenium and zinc-enriched Hailan brown laying hens and a preparation method thereof. After adding ice, yeast selenium, and zinc methionine to the additive, the core-shell structure of the additive is divided into an inner core and an outer layer. The inner core includes quince extract, ice, and yeast selenium, while zinc methionine is added to the outer layer. At the same time, the ice molecules are wrapped in the microemulsion form of quince extract, and the yeast selenium is dispersed in the interstitial space of the microemulsion, thereby blocking the direct contact between the two, and solving the problems raised in the background art, i.e.:

[0005] After adding ice, yeast selenium, and zinc methionine to the additive in the prior art, the direct mixing of the three generates complex precipitates and intestinal absorption antagonism, which can cause the loss of anti-inflammatory and absorption promotion functions, and the competition for absorption carriers in the intestinal tract between selenium and zinc, which can easily cause thinning of the eggshell and decline of the immune function of laying hens.

[0006] To achieve the above-mentioned purpose, the functional feed additive for selenium and zinc-enriched Hailan brown laying hens is composed of the following raw materials: yeast selenium, zinc methionine, ice, quince extract, dog spine extract, cistanche extract, angelica extract, spider herb-typhonium giganteum fermented extract, figwort oligosaccharide extract, and licorice extract.

[0007] The additive is a core-shell structure particle, the inner core of the core-shell structure particle comprises quince extract, borneol and yeast selenium;

[0008] The outer layer of the core-shell structure particle comprises zinc methionine, rhizoma ginseng extract, cistanche extract, angelica extract, spider-typhonium fermented extract, ficus pumila oligosaccharide extract and licorice extract;

[0009] The quince extract in the inner core is in a microemulsion form with a particle size of ≤100 nm.

[0010] In the above technical solution, the additive is a core-shell structure particle, the inner core of which comprises quince extract, borneol and yeast selenium, and the quince extract is in a microemulsion form with a particle size of ≤100 nm. The hydrophobic inner core in the microemulsion form efficiently encapsulates borneol molecules, and the hydrophilic outer phase disperses yeast selenium particles in the microemulsion gap to form a spatial isolation barrier. This structure blocks the molecular-level contact between borneol and yeast selenium, effectively overcomes the thermodynamic repulsion caused by the difference in physical and chemical properties of the two, and ensures the physical stability of the inner core components during processing and storage.

[0011] The amphiphilic long-chain structure of the quince extract in the microemulsion phase wraps borneol molecules, significantly reducing the surface free energy thereof, so that the volatility of borneol is attenuated to less than 1 / 3 of that of conventional preparations. At the same time, the water core-oil shell double-phase structure of the microemulsion provides an inert environment for yeast selenium, blocks the erosion of water and oxygen molecules on the active center of organic selenium, improves the thermal stability of yeast selenium, and ensures the chemical integrity of the active ingredients during feed processing.

[0012] The intestinal targeting property of the inner core microemulsion and the core-shell slow-release design form a synergy. The outer layer zinc methionine is preferentially released in the gastric acid environment, inducing the up-regulation of intestinal zinc transporter expression. The inner core borneol-yeast selenium is slowly released in the intestinal alkaline environment, and by then, zinc ions have been absorbed and transported, and yeast selenium can be efficiently taken up through an independent channel.

[0013] Secondly, the raw materials comprise the following components in terms of weight percentage:

[0014] Yeast selenium, 0.1%-0.3%;

[0015] Zinc methionine, 0.2%-0.5%;

[0016] Borneol, 8%-12%;

[0017] Quince extract, 20%-28%;

[0018] Rhizoma ginseng extract, 11%-15%;

[0019] Cistanche extract, 8%-11%;

[0020] Angelica sinensis extract, 15%-18%;

[0021] Valeriana jatamansi-Acorus calamus ferment extract, 8%-15%;

[0022] Ficus tikvea oligosaccharide extract, 10%-11%;

[0023] Glycyrrhiza extract, 5%-6%;

[0024] The weight percentage is based on the total weight of the feed additive.

[0025] In the above component ratio, the trace setting of selenium yeast and zinc methionine not only meets the daily selenium and zinc nutritional needs of laying hens, but also avoids the absorption antagonism caused by high concentration ions; the critical encapsulation concentration of borneol in the microemulsion structure constructed by serviceberry extract is reached, a stable oil-water partition coefficient is formed, and the slow release of anti-inflammatory ingredients is ensured while inhibiting the complexation of trace elements; the extract of Cibotium barometz, the extract of Cistanche and the extract of Angelica sinensis constitute the kidney-tonifying and blood-activating functional group, which synergistically strengthens the synthesis of eggshell matrix; the bitter taste degradation characteristics of Valeriana jatamansi-Acorus calamus ferment extract and the prebiotic effect of Ficus tikvea oligosaccharide form a foraging promotion combination; the saponin of Glycyrrhiza extract as a natural adhesive and immunomodulator can enhance the effect while buffering the flavor conflict of each component.

[0026] On this basis, the Valeriana jatamansi-Acorus calamus ferment extract is prepared by Bacillus subtilis fermentation.

[0027] In addition, the raw materials also contain vitamin D3 5000-6000 IU / kg and vitamin E 200-250 mg / kg;

[0028] The extract of Cibotium barometz, the extract of Cistanche and the extract of Angelica sinensis in the outer layer are embedded by sodium alginate-chitosan gel and sprayed with an ethyl cellulose moisture-proof layer;

[0029] The molecular weight of the Ficus tikvea oligosaccharide extract is ≤3000 Da.

[0030] Secondly, the application provides a preparation method of the functional feed additive for selenium and zinc-rich Hailan brown laying hens, which comprises the following steps:

[0031] S1, preparation of outer layer slurry:

[0032] The extract of Cibotium barometz, the extract of Cistanche and the extract of Angelica sinensis are mixed according to the ratio, and water is added to prepare a slurry with a solid content of 20%-60%, which is mixed with 1%-10% sodium alginate solution according to a mass ratio of 1:2;

[0033] The mixed slurry is dropped into 1%-2% chitosan solution containing 1%-4% calcium chloride, and the gel microspheres are formed after solidification for 20-40 minutes;

[0034] S2, mix the gel microspheres with the Ferula sinkiangensis- Acorus calamus fermented extract, Ficus tikvea oligosaccharide extract, licorice extract, and zinc methionine according to a ratio, and add vitamin D3 and vitamin E powder;

[0035] S3, crush the mixed material through an 80-mesh sieve, use a fluidized bed to spray 5%-15% ethyl cellulose ethanol solution to form a moisture-proof layer, and add 1%-10% hydroxypropyl methyl cellulose solution to prepare a coating slurry;

[0036] S4, inner core preparation:

[0037] Homogenize the quince extract microemulsion with borneol and yeast selenium according to a ratio, and spray dry at an inlet temperature of 40-80 DEG C to obtain the inner core of the core-shell structure particles;

[0038] S5, core-shell coating:

[0039] Place the inner core of the core-shell structure particles in S4 in a fluidized bed, and use the coating slurry in S3 to spray and coat the inner core of the core-shell structure particles, at an inlet temperature of 40-45 DEG C, and a coating weight gain of 30%-35%;

[0040] After the spray coating, dry at 40-50 DEG C or below until the moisture content is less than or equal to 5%, to obtain the finished core-shell structure particles.

[0041] Compared with the prior art, the beneficial effects of the present application are:

[0042] After adding borneol, yeast selenium and zinc methionine to the additive, the core-shell structure of the additive is divided into an inner core and an outer layer, the inner core includes quince extract, borneol and yeast selenium, and the zinc methionine is added to the outer layer, at the same time, the borneol molecules are wrapped by the microemulsion form of the quince extract, and the yeast selenium is dispersed in the interstitial space of the microemulsion, thereby blocking the direct contact between the two, blocking the precipitation of zinc complexation with borneol, and at the same time, the zinc methionine embedded by the outer layer of sodium alginate-chitosan gel is preferentially released in the stomach acid environment to induce the expression of zinc transporter, so that the borneol and yeast selenium in the inner core are released slowly in the intestinal alkaline environment to realize the time sequence separation of selenium and zinc absorption. BRIEF DESCRIPTION OF DRAWINGS

[0043] Figure 1 It is a component schematic diagram in Example 1 of the present application;

[0044] Figure 2 It is a preparation method flow chart of the additive in Example 1 of the present application. DETAILED DESCRIPTION

[0045] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative work fall within the protection scope of the present application.

[0046] At present, after the existing technology adds borneol, yeast selenium and zinc methionine in the additive, the complex precipitate and intestinal absorption antagonism generated after the direct mixing of the three will cause the loss of anti-inflammatory and absorption-promoting functions, and the competition for absorption carriers of selenium and zinc in the intestinal tract will easily cause the thinning of eggshells and the decline of the immunity of laying hens. The present application provides a functional type feed additive for selenium-rich and zinc-rich Hailan brown laying hens, which is composed of the following raw materials: yeast selenium, zinc methionine, borneol, quince extract, rhizoma cibotii extract, cistanche deserticola y. matsus. extract, angelica sinensis (oliv.) diels extract, ferula sinkiangensis k. m. kegs-fermented extract, ficus tikvea oligosaccharide extract and licorice extract. Figure 1 As shown in the formula, the additive is composed of the following raw materials: yeast selenium, zinc methionine, borneol, quince extract, rhizoma cibotii extract, cistanche deserticola y. matsus. extract, angelica sinensis (oliv.) diels extract, ferula sinkiangensis k. m. kegs-fermented extract, ficus tikvea oligosaccharide extract and licorice extract.

[0047] The additive is a core-shell structure particle, and the inner core of the core-shell structure particle comprises quince extract, borneol and yeast selenium.

[0048] The outer layer of the core-shell structure particle comprises zinc methionine, rhizoma cibotii extract, cistanche deserticola y. matsus. extract, angelica sinensis (oliv.) diels extract, ferula sinkiangensis k. m. kegs-fermented extract, ficus tikvea oligosaccharide extract and licorice extract.

[0049] Embodiment 1

[0050] In the core-shell structure particle of the additive of the present embodiment, the proportion of the inner core components is: quince 28%, borneol 8% and yeast selenium 0.1%; the proportion of the outer layer components is: zinc methionine 0.2%, rhizoma cibotii 11%, cistanche deserticola y. matsus. 8%, angelica sinensis (oliv.) diels 15%, fermented extract 8%, oligosaccharide 10% and licorice 5%; vitamin D3 5500 IU / kg, vitamin E 225 mg / kg, at the same time, the quince extract is in the form of microemulsion with a particle size of 80 nm, the coating weight is increased by 30%, and the coating weight test method is to dry the particles after coating to a constant weight, and calculate the percentage increase in mass.

[0051] Preparation method

[0052] S1, mix rhizoma cibotii extract, cistanche deserticola y. matsus. extract and angelica sinensis (oliv.) diels according to the proportion, and adjust the slurry to a solid content of 40% by adding water, and mix with 5% sodium alginate solution according to a mass ratio of 1:2;

[0053] Drop the mixed slurry into a 1.5% chitosan solution containing 2% calcium chloride, and solidify for 30 minutes to form gel microspheres, and the solidification condition parameters are: constant temperature stirring at 25℃, rotation speed 200 rpm;

[0054] S2, mix the gel microspheres with the Ferula sinkiangensis K.M. Shen and Acorus calamus L. fermentation extract, Ficus tikveus Hance oligosaccharide extract, licorice extract, and zinc methionine according to a ratio, and add vitamin D3 and vitamin E powder;

[0055] S3, crush the mixture to pass through an 80-mesh sieve, use a fluidized bed to spray 10% ethyl cellulose ethanol solution to form a moisture-proof layer, and add 5% hydroxypropyl methylcellulose solution to prepare a coating slurry;

[0056] S4, homogenize the quince extract microemulsion with borneol and yeast selenium according to a ratio, and spray dry at an inlet temperature of 60°C to obtain the inner core of the core-shell structure particle, wherein the preparation method of the quince extract microemulsion comprises: mixing quince extract, ethyl oleate (oil phase), and Tween 80 according to a mass ratio of 10:3:1, adding 3 times the volume of pure water, and treating at 55°C and 15000 psi under high pressure homogenization for 5 cycles to obtain quince extract microemulsion with a particle size of ≤100 nm, the particle size of the quince extract microemulsion is detected by dynamic light scattering, the Zeta potential is -35mV to -40mV, and the particle size fluctuation after the borneol is encapsulated is ≤5%;

[0057] The formation mechanism of the quince extract microemulsion is that the triterpenoid saponins in the quince extract and Tween 80 cooperatively reduce the oil-water interfacial tension to ≤2mN / m, so that the ethyl oleate forms nanodroplets under high shear; the negative charge layer of the microemulsion maintains the stability of the system through electrostatic repulsion, the borneol molecules are encapsulated in the hydrophobic core, and the yeast selenium is dispersed in the continuous water phase gap;

[0058] S5, place the inner core of the core-shell structure particle in S4 in a fluidized bed, and spray coat the inner core of the core-shell structure particle with the coating slurry in S3, at an inlet temperature of 40°C, and a coating weight gain of 30%;

[0059] After the spray coating, dry at 45°C or below until the moisture content is ≤5%, to obtain the core-shell structure particle finished product.

[0060] Example 2:

[0061] In the core-shell structure particle of the additive of this example, the ratio of the inner core components is: quince 20%, borneol 12%, and yeast selenium 0.3%; the ratio of the outer layer components is: zinc methionine 0.5%, dog spine 15%, cistanche 11%, angelica 18%, fermentation extract 15%, oligosaccharide 11%, and licorice 6%; at the same time, the quince extract is in the form of a microemulsion with a particle size of 100 nm, and the coating weight gain is 35%.

[0062] Example 3:

[0063] The core-shell structure particle of the additive of the present embodiment has the following proportions of components: 24% quince, 10% borneol, and 0.2% yeast selenium for the inner core component; and 0.35% zinc methionine, 13% dog spine, 9.5% cistanche, 16.5% angelica, 12% fermentation extract, 10.5% oligosaccharide, and 5.5% licorice for the outer layer component. Meanwhile, the quince extract is in the form of microemulsion with a particle size of 50 nm, and the coating weight is increased by 32%.

[0064] Comparative Example 1

[0065] The proportions of the components in the additive are the same as in Example 1, and the difference from Example 1 is that the core-shell structure is cancelled, and all the raw materials are directly mixed.

[0066] Comparative Example 2

[0067] The proportions of the components in the additive and the preparation method are the same as in Example 1, and the difference from Example 1 is that the inner core of the core-shell structure particle uses an equal amount of malt dextrin instead of quince extract, and borneol and yeast selenium are directly mixed and then spray dried.

[0068] Comparative Example 3

[0069] The proportions of the components in the additive and the preparation method are the same as in Example 1, and the difference from Example 1 is that the dog spine extract, cistanche extract, and angelica extract in the outer layer are not gel-embedded using sodium alginate-chitosan.

[0070] Experimental Example 1: Complex Precipitation Blocking Verification

[0071] Verification Method:

[0072] Simulated gastric juice (0.1M HCl, pH 2.0) treatment for 2h;

[0073] The precipitate was collected using a 0.22μm filter membrane, and the zinc content was measured by AAS;

[0074] Borneol residual rate: GC-MS detection of supernatant.

[0075] Table 1: Results of complex precipitation blocking verification experiment

[0076] Group Zinc content of precipitate (mg / g) Residual rate of ice flakes (%) Ice flakes-zinc contact probability Example 1 Not detected 96.3±0.8 0.3％ Example 2 Not detected 95.1±1.2 0.4％ Example 3 Not detected 97.2±0.5 0.2％ Comparative Example 1 38.7±1.5 38.0±3.1 98.7％

[0077] Explanation: The contact probability of borneol-zinc was quantitatively analyzed by molecular dynamics simulation (COMPASS II force field, 500ns gastric juice environment) to analyze the spatial distribution of borneol and zinc ions in quince microemulsion, and the contact probability was calculated by atomic distance threshold.

[0078] Conclusion: Based on the precipitation detection in simulated gastric fluid environment and the results of molecular dynamics simulation, in Example 1 to Example 3, the Borneol-Zinc contact probability is inhibited to ≤0.4% by the steric hindrance effect of the quince microemulsion system, while the contact probability in Comparative Example 1 is 98.7%, making the zinc content of the precipitate in Example 1 to Example 3 undetectable, negligible, and the residual rate of borneol ≥95.1%; while in Comparative Example 1, because the core-shell structure of the additive is cancelled, the residual rate of borneol is ≤38.0% while the zinc content of the precipitate is 38.7±1.5 mg / g, which shows that the core-shell structure of the additive provided in the scheme wraps the borneol molecules in the microemulsion form of quince extract, and disperses the yeast selenium in the microemulsion gap, blocking the direct contact of the two, and blocking the formation of borneol-zinc complex precipitation.

[0079] Experimental Example 2: Time sequence separation of selenium-zinc absorption

[0080] Verification method:

[0081] 1. In the gastric simulation stage (0-2h), perfusion 65 Zn-labeled simulated gastric fluid quantifies zinc absorption, and switches in the intestinal simulation stage (2-6h) 75 Se-selenomethionine simulated intestinal fluid for selenium absorption;

[0082] 2. Western Blot (Abcam ab28632 antibody detection) combined with protein blotting to quantify intestinal mucosa ZIP4 transporter expression;

[0083] 3. Finally, through the synergistic analysis of the competition coefficient and the ZIP4 expression fold change, the absorption of zinc ions in the stomach segment is verified.

[0084] Table 2: Results of selenium-zinc absorption verification experiment

[0085] Group Zinc absorption rate of stomach section Selenium absorption rate of intestine section Selenium / zinc competition coefficient ZIP4 expression amount (times) Example 1 88.7±2.9％ 75.8±3.1％ 0.11 2.4±0.2 Example 2 89.2±3.1％ 76.5±2.8％ 0.12 2.3±0.3 Example 3 90.1±2.5％ 77.3±2.6％ 0.10 2.6±0.2 Comparative Example 3 94.5±2.7％ 42.3±3.5％ 0.87 1.0±0.1

[0086] Conclusion: According to the experimental data results shown in Table 2, in Example 1-3, the zinc absorption rate in the stomach segment is maintained at 88.7% or more, and the selenium absorption rate in the intestinal segment is maintained at 75.8% or more. In addition, although in Comparative Example 3, the spine extract, cistanche extract and angelica extract are not embedded with sodium alginate-chitosan gel, the zinc absorption rate in the stomach segment is 94.5%, but the selenium absorption rate in the intestinal segment is only 42.3%, the fundamental reason is that after embedding with sodium alginate-chitosan gel in Example 1-3, the intestinal mucosa ZIP4 transporter expression is induced to up-regulate, significantly weakening the competition of selenium-zinc absorption, and finally achieving the time sequence separation and synergistic effect of selenium-zinc ions in the stomach and intestinal stages, so that the absorption rate of selenium-zinc ions in the stomach and intestinal stages can be at a high level.

[0087] Experimental Example 3: Comprehensive feeding effect

[0088] Verification method:

[0089] 360 Hy-Line Brown laying hens of 28 weeks old were randomly divided into 6 groups, 60 in each group;

[0090] The basic diet was used at the same time, among which three groups added the additives of examples 1-3, another two groups used comparative example 1 and conventional additives respectively, and the last group did not use additives as a blank control group;

[0091] Detection standard: eggshell strength (GB / T 5009.6) and selenium content in egg (GB 5009.93).

[0092] Table 3: Quality detection data of eggs after feeding

[0093] Group Eggshell strength (N) Egg yolk selenium (μg / g) Feed / egg ratio Broken egg rate Salmonella positive rate Example 1 41.8±1.5 0.84±0.04 2.10±0.06 1.2％ 7.5％ Example 2 42.3±1.6 0.86±0.05 2.08±0.07 0.9％ 6.7％ Example 3 42.0±1.4 0.85±0.03 2.09±0.05 1.0％ 7.0％ Comparative Example 1 35.2±2.1 0.31±0.03 2.35±0.11 8.7％ 23.3％ Conventional additive 37.8±1.9 0.52±0.04 2.22±0.09 4.2％ 15.0％ Blank control group 36.5±2.0 0.18±0.02 2.40±0.10 9.3％ 28.7％

[0094] Conclusion: As shown in the experimental results of Table 3, the core-shell structure additive used in examples 1-3, after being added to the basic diet of laying hens, the eggs laid by the laying hens are superior to comparative example 1, conventional additives and blank control group in many indexes such as eggshell strength, egg yolk selenium, feed egg ratio, broken egg rate and salmonella positive rate, among which the eggshell strength is significantly improved to 41.8-42.3N, the egg yolk selenium enrichment reaches 0.84-0.86μg / g, confirming that examples 1-3 through the selenium-zinc time sequence absorption strategy, synchronously strengthen the structure strength, nutritional quality and biological safety of egg products.

[0095] Experimental example 4: verification of dissolution characteristics of core-shell structure

[0096] Test conditions:

[0097] Simulated gastric juice: 0.1M HCl (pH 2.0, 37℃);

[0098] Simulated intestinal juice: PBS containing 0.5% cholate (pH 7.4, 37℃);

[0099] Detection method: AAS for zinc, HPLC for borneol, ICP-MS for selenium.

[0100] Table 4: Verification of dissolution characteristics of core-shell structure

[0101]

[0102]

[0103] Conclusion: According to the experimental verification results shown in Table 4, the quince microemulsion system of Examples 1-3 achieves efficient zinc release in simulated gastric fluid, and the simultaneous ice piece dissolution rate in the simulated intestinal fluid stage is 85.6-87.2% and the selenium yeast release rate is 83.4-84.7%. Compared with Comparative Examples 1 and 3, which lack the core-shell controlled release capability, the intestinal ice piece / selenium release rate drops to 43.2-52.4% and 37.8-48.9%, respectively, accompanied by significant zinc precipitation, which fully confirms that the core-shell structure induces rapid zinc release in the stomach to induce absorption channels, and cooperates with the slow release of ice pieces and selenium elements in the intestine to achieve the time-space precise delivery of active ingredients.

[0104] Experimental Example 5: Verification of the moisture-proof layer stability

[0105] Test method:

[0106] The finished additive of Example 1 was divided into two groups:

[0107] Group A: containing ethyl cellulose moisture-proof layer

[0108] Group B: without adding a moisture-proof layer

[0109] Accelerated storage at 40±2℃, 75% RH for 3 months, and detection of changes in the residual rate of borneol, the activity retention rate of selenium yeast, the zinc dissolution rate in gastric fluid, and the dissolution rate of borneol in intestinal fluid.

[0110] Table 5: Effect of the moisture-proof layer on the stability of the additive

[0111] Group Residual rate of ice flakes Active retention rate of yeast selenium Zinc dissolution rate in gastric juice Ice flakes dissolution rate in intestinal juice Group A 95.2％±1.1％ 91.7％±2.3％ 90.8％±1.7％ 84.3％±1.9％ Group B 68.4％±3.5％ 52.6％±4.1％ 76.2％±3.8％ 49.7％±3.2％

[0112] Conclusion: According to the test results shown in Table 5, the core indicators of Group A after adding the ethyl cellulose moisture-proof layer are better than those without adding the ethyl cellulose moisture-proof layer, with a borneol residual rate of 95.2%, a selenium yeast activity retention rate of 91.7%, a zinc dissolution rate in gastric fluid of 90.8%, and an intestinal borneol dissolution rate of 84.3%, which proves that the ethyl cellulose moisture-proof layer can significantly inhibit the volatilization of borneol and the degradation of active ingredients in a humid environment, and ensure the controlled release function of the core-shell structure during the storage period.

[0113] The above shows and describes the basic principles, main features and advantages of the present application. Those skilled in the art should understand that the present application is not limited to the above examples, and the above examples and descriptions in the specification are only preferred examples of the present application and are not intended to limit the present application. Without departing from the spirit and scope of the present application, various changes and improvements can be made to the present application, and these changes and improvements all fall within the scope of the claimed present application. The scope of protection of the present application is defined by the appended claims and their equivalents.

Claims

1. A selenium- and zinc-enriched functional feed additive for Hy-Line Brown laying hens, characterized in that, Composed of the following ingredients: yeast selenium, zinc methionine, borneol, quince extract, Cibotium barometz extract, Cistanche deserticola extract, Angelica sinensis extract, spider lily-Fennel and Acorus calamus fermented extract, Ficus hirta oligosaccharide extract, and licorice extract; The additive is a core-shell structured particle, the core of which contains quince extract, borneol and yeast selenium; The outer layer of the core-shell structured particles contains zinc methionine, Cibotium barometz extract, Cistanche deserticola extract, Angelica sinensis extract, Acorus calamus-Fennel fermented extract, Ficus hirta oligosaccharide extract, and Glycyrrhiza uralensis extract.

2. The selenium- and zinc-enriched Hy-Line Brown functional feed additive for laying hens according to claim 1, characterized in that, The raw materials, by weight percentage, comprise the following components: Yeast selenium, 0.1%-0.3%; Zinc methionine, 0.2%-0.5%; Borneol, 8%-12%; Quince extract, 20%-28%; Cibotium barometz extract, 11%-15%; Cistanche deserticola extract, 8%-11%; Angelica sinensis extract, 15%-18%; Spider fragrance - fermented extract of fennel and calamus, 8%-15%; Oligosaccharide extract of Prunus pubescens, 10%-11%; Licorice extract, 5%-6%; The weight percentage is based on the total weight of the feed additives.

3. The selenium- and zinc-enriched Hy-Line Brown functional feed additive for laying hens according to claim 1, characterized in that: The quince extract in the core is in the form of a microemulsion with a particle size ≤100nm.

4. The selenium- and zinc-enriched Hy-Line Brown functional feed additive for laying hens according to claim 1, characterized in that: The spider fragrance-fennel calamus fermentation extract was obtained by fermentation with Bacillus subtilis.

5. The selenium- and zinc-enriched Hy-Line Brown functional feed additive for laying hens according to claim 1, characterized in that: The raw materials also contain 5000-6000 IU / kg of vitamin D3 and 200-250 mg / kg of vitamin E.

6. The selenium- and zinc-enriched Hy-Line Brown functional feed additive for laying hens according to claim 1, characterized in that: The outer layer contains Cibotium barometz extract, Cistanche deserticola extract, and Angelica sinensis extract, which are encapsulated in sodium alginate-chitosan gel and coated with an ethyl cellulose moisture-proof layer.

7. The selenium- and zinc-enriched Hy-Line Brown functional feed additive for laying hens according to claim 1, characterized in that: The molecular weight of the oligosaccharide extract of Prunus pubescens is ≤3000 Da.

8. A method for preparing the selenium- and zinc-enriched Hy-Line Brown functional feed additive for laying hens according to any one of claims 1-7, characterized in that, Includes the following steps: S1. Preparation of outer layer slurry: The extracts of Cibotium barometz, Cistanche deserticola, and Angelica sinensis are mixed in a certain proportion and then diluted with water to form a slurry with a solid content of 20%-60%. This slurry is then mixed with a 1%-10% sodium alginate solution at a mass ratio of 1:

2. The mixed slurry is dropped into a 1%-2% chitosan solution containing 1%-4% calcium chloride and cured for 20-40 minutes to form gel microspheres; S2. Mix the gel microspheres with the fermented extracts of spider lily and calamus, oligosaccharide extract of five-finger peach, licorice extract, and zinc methionine in a certain proportion, and add vitamin D3 and vitamin E powder. S3. Crush the mixture through an 80-mesh sieve, use a fluidized bed to spray a 5%-15% ethyl cellulose ethanol solution to form a moisture-proof layer, and add a 1%-10% hydroxypropyl methyl cellulose solution to make a coating slurry.

9. The preparation method according to claim 8, characterized in that, It also includes the following steps: S4, Kernel Preparation: The quince extract microemulsion was homogenized with borneol and yeast selenium in a certain ratio, and spray-dried at an inlet air temperature of 40-80℃ to obtain the core of the core-shell structured particles.

10. The preparation method according to claim 8, characterized in that, It also includes the following steps: S5, Core-shell coating: The core of the core-shell structured particles in S4 is placed in a fluidized bed, and the core of the core-shell structured particles is coated with the coating slurry in S3. The air inlet temperature is 40-45℃, and the coating weight gain is 30%-35%. After spray coating, the particles are dried at 40-50℃ until the moisture content is ≤5% to obtain the core-shell structured particles.

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