Sweat collection-detection integrated system, use method thereof and sweat collection device

Abstract

The invention relates to the technical field of sweat detection, in particular to a sweat collection-detection integrated system, a use method thereof and a sweat collection device. The integrated system comprises a sweat collecting device, the sweat collecting device comprises a base material layer and a sweat collecting assembly arranged on the base material layer, the sweat collecting assembly comprises a sweat conveying platform and a liquid storage platform which are coaxially nested in sequence from outside to inside, the sweat conveying platform and the liquid storage platform are detachably connected, and the liquid storage platform is coaxially provided with a liquid storage groove; a plurality of micro-channels are formed in the surface, facing the skin, of the sweat transmission platform, flow guide channels which are communicated with the micro-channels and correspond to each other are formed in the surface of the liquid storage platform, and sweat flows into the liquid storage groove along the micro-channels and the flow guide channels. And a three-electrode system for detecting a biomarker in sweat in the liquid storage tank in real time is arranged on the liquid storage tank. By optimizing the geometrical shape of the micro-channel, surface treatment, wettability gradient driving and anti-blocking design, sweat can be efficiently and stably collected and detected.

Description

Technical Field

[0001] This invention relates to the field of sweat detection technology, and in particular to an integrated sweat collection and detection system and its usage method, as well as a sweat collection device. Background Technology

[0002] Sweat, as an important physiological fluid of the human body, contains rich biochemical information. Its components (such as electrolytes [sodium chloride, potassium ions], metabolites [lactic acid, urea], hormones [cortisol], and drug metabolites) can directly or indirectly reflect the physiological state, metabolic level, and pathological changes of the human body.

[0003] In recent years, with the development of wearable devices and point-of-care testing (POCT) technology, dynamic health monitoring based on sweat has become a research hotspot in the biomedical field. For example, monitoring lactic acid concentration in sweat can assess exercise intensity and muscle fatigue; analyzing electrolyte levels (such as sodium and potassium) can aid in the diagnosis of dehydration or electrolyte imbalances; and even trace biomarkers in sweat (such as cortisol) can screen for stress-related diseases or endocrine abnormalities. Compared to blood tests, sweat has advantages such as non-invasive collection and continuous monitoring, thus showing broad application prospects in sports medicine, chronic disease management (such as diabetes and hypertension), rapid on-site assessment in emergency situations, and daily health management for healthy individuals.

[0004] However, to achieve effective sweat monitoring, the first step is to solve the problem of efficient and stable sweat collection. Currently, existing technologies mainly rely on two conventional methods for sweat collection: passive diffusion and gravity drainage.

[0005] The core principle of passive diffusion is to utilize the concentration difference or capillary action between sweat and the collection medium (such as absorbent cotton or superabsorbent polymer), allowing sweat to naturally permeate into the collection medium. This method requires no external power, has a simple structure, and is often used in low-tech scenarios (such as the sweat-absorbing layer in traditional sports protective gear). However, the method also has significant drawbacks: (1) The diffusion rate of sweat is limited by factors such as concentration gradient, medium porosity and environmental humidity. Especially in low sweating scenarios (such as daily light activities or normal temperature environment), the sweat generation rate is slow and the diffusion process takes several hours or even longer, which cannot meet the needs of real-time or rapid monitoring; (2) The diffusion process is random. The distribution of sweat on the surface of the medium is easily affected by the roughness of the skin surface, the density of sweat gland distribution (the density of sweat glands in different parts of the human body can vary by more than 10 times) and changes in body position (such as the change in the direction of sweat flow when the arm is hanging down and raised horizontally), which makes the collected sample unable to accurately reflect the overall sweat composition of the target area; (3) If the collection medium is exposed to the air, the volatile components in the sweat (such as ethanol and acetone) are easily lost, while water evaporation will cause the sample to concentrate, affecting the accuracy of subsequent detection.

[0006] Gravity drainage method uses gravity to drive sweat from the skin surface to the collection container by designing inclined drainage channels (such as grooves or microchannels). Compared with passive diffusion method, this method can shorten the collection time and is suitable for certain sweating scenarios (such as moderate-intensity exercise). However, it still has the following key problems: (1) In addition to water, sweat also contains salt (such as sodium chloride, with a concentration of 0.5-1%), urea (about 2%), and particulate matter such as shed skin cells and sebaceous gland secretions. These components are easy to deposit in the narrow parts of the drainage channel (such as the corner of the microchannel, the interface between the channel and the skin), which leads to channel blockage. The blockage problem is more prominent, especially when wearing for a long time or in a high humidity environment (such as when sweating increases greatly in summer); (2) Gravity drainage depends on the continuous generation and flow of sweat. When the amount of sweat is insufficient (such as light sweating or a static state), the sweat cannot overcome the resistance of the drainage channel (such as surface tension and contact angle), which leads to the sample in the collection container being blocked. This amount cannot meet the detection requirements (some detection methods require at least a few microliters to tens of microliters of sample); (3) The design of the diversion channel (such as width, depth, distribution density) directly affects the sweat collection efficiency. If the channel layout does not match the distribution of human sweat glands (such as the high density of sweat glands on the forehead and back of the human body, while the density of sweat glands at the ends of the limbs is low), it is easy to cause local areas of sweat not to be effectively collected, resulting in sample deviation; In addition, gravity drainage method usually requires the collection device to be fixed on the skin surface and kept at a specific angle (such as tilted at 15-30 degrees). Otherwise, the weakening of gravity will lead to a sharp drop in collection efficiency, which puts forward higher requirements for the wearing comfort of wearable devices and limits their application in daily scenarios.

[0007] In conclusion, given the shortcomings of traditional sweat collection methods in terms of efficiency, clogging risk, sample size, and uniformity, developing a novel sweat collection device has significant practical implications and application value. Summary of the Invention

[0008] In view of the shortcomings of the prior art described above, the purpose of this invention is to provide an integrated sweat collection and detection system and its usage method, as well as a sweat collection device, to solve the problems of clogging, insufficient sample volume, and uneven collection that may exist in the sweat collection equipment of the prior art.

[0009] To achieve the above and other related objectives, the present invention is obtained through the following technical solution.

[0010] The first aspect of the present invention is to provide a sweat collection device, the collection device comprising a substrate layer for covering and conforming to the skin and a sweat collection component disposed on the substrate layer, the sweat collection component comprising a sweat transmission platform and a liquid storage platform coaxially nested from the outside to the inside, the sweat transmission platform and the liquid storage platform being detachably connected, the liquid storage platform having a liquid storage tank coaxially formed thereon, the sweat transmission platform having a plurality of microchannels formed on the surface facing the skin, and the liquid storage platform having a guide channel communicating with and corresponding to the microchannels on its surface, the sweat flowing into the liquid storage tank along the microchannels and the guide channel.

[0011] A second aspect of the present invention is to provide an integrated sweat collection and detection system, including the sweat collection device as described above.

[0012] A third aspect of the present invention provides a method of using an integrated sweat collection and detection system, comprising the following steps:

[0013] (1) The surface of the sweat delivery platform containing microchannels is attached to the skin;

[0014] (2) When the sweat-inducing electrode is energized, an electric current is formed on the skin surface between the cathode and the anode, and the sweat-inducing substance in the sweat transport platform enters the skin to produce sweat.

[0015] (3) Sweat enters the microchannel through the surface of the sweat transfer platform, and then flows into the guide channel along the microchannel and enters the storage tank;

[0016] (4) The three-electrode system detects biomarkers in sweat in the storage tank in real time.

[0017] The fourth aspect of the present invention provides the application of the sweat collection device and / or the sweat collection-detection integrated system as described above in sweat detection.

[0018] As described above, the sweat collection-detection integrated system and its usage method, as well as the sweat collection device of the present invention, have the following beneficial effects:

[0019] This invention provides an efficient and stable solution for sweat collection and detection by optimizing the geometry, surface treatment, wettability gradient drive, and anti-clogging design of the surface channels of the sweat transfer platform. The microchannel design and its integrated system can significantly improve sweat collection efficiency, reduce sample collection time, and ensure the stability and consistency of the collection process. Attached Figure Description

[0020] Figure 1 The diagram shown is a schematic representation of the sweat collection device of the present invention.

[0021] Figure 2 The image shown is a bottom view of the sweat collection device of the present invention.

[0022] Figure 3 The image shown is a cross-sectional view of the sweat collection device of the present invention.

[0023] Figure 4 The diagram shown is a structural schematic of the sweat collection and detection integrated system of the present invention.

[0024] Figure 5 The diagram shows a sweat drainage process in the sweat collection device of the present invention.

[0025] Explanation of reference numerals in the attached figures

[0026] 1. Substrate layer; 2. Sweat transfer platform; 21. Microchannel; 3. Liquid storage platform; 31. Flow channel; 32. Liquid storage tank; 33. Connection hole; 4. Sweat excretion promotion component; 41. Cathode; 42. Anode; 5. Three-electrode system; 51. Working electrode; 52. Counter electrode; 53. Reference electrode. Detailed Implementation

[0027] To make the inventive objectives, technical solutions, and beneficial effects of this invention clearer, the invention will be further described in detail below with reference to embodiments. Those skilled in the art can easily understand other advantages and effects of this invention from the content disclosed in this specification.

[0028] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. The terminology used herein in the specification of this invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0029] In this invention, coaxial nesting means that the axes of the sweat transfer platform and the liquid storage platform overlap.

[0030] The first aspect of the present invention is to provide a sweat collection device, the collection device comprising a substrate layer 1 for covering and conforming to the skin and a sweat collection assembly disposed on the substrate layer 1, the sweat collection assembly comprising a sweat transmission platform 2 and a liquid storage platform 3 coaxially nested from the outside to the inside, the sweat transmission platform 2 and the liquid storage platform 3 being detachably connected, the liquid storage platform 3 having a liquid storage tank 32 coaxially formed therein, the sweat transmission platform 2 having a plurality of microchannels 21 formed on the surface facing the skin, and the liquid storage platform 3 having a guide channel 31 communicating with and corresponding to the microchannels 21 on the surface, the sweat flowing into the liquid storage tank 32 along the microchannels 21 and the guide channel 31.

[0031] The sweat transmission platform 2 of this invention has multiple microchannels 21, and a liquid storage platform 3 is nested inside the sweat transmission platform 2. Sweat is transmitted through the microchannels 21 and collected by the liquid storage platform 3. This increases the amount of sweat collected and prevents sweat accumulation. It also facilitates subsequent skin contact, making it easier to stimulate sweating and perform in-situ detection of sweat.

[0032] In some embodiments of the present invention, the cross-section of the sweat transfer platform 2 is not particularly limited, and a cross-section commonly used by those skilled in the art for the purpose of sweat transfer platform 2 can be adopted. Specifically, the cross-section of the sweat transfer platform 2 of the present invention is annular. In order to make the outer surface of the liquid storage platform 3 better fit the inner surface of the sweat transfer platform 2, the cross-section of the liquid storage platform 3 is circular and coincides with the center of the circle of the sweat transfer platform 2.

[0033] In some embodiments of the present invention, the height of the sweat transmission platform 2 is not specifically limited, and the height of the sweat transmission platform 2 commonly used by those skilled in the art can be adopted. Specifically, the height of the sweat transmission platform 2 is 1.9-2.1 mm, preferably 2 mm.

[0034] In some embodiments of the present invention, the height of the liquid storage platform 3 is not particularly limited, and the height of the liquid storage platform 3 commonly used by those skilled in the art can be adopted, wherein the height of the liquid storage platform 3 is less than or equal to the height of the sweat transfer platform 2.

[0035] Specifically:

[0036] When the height of the liquid storage platform 3 is less than the height of the sweat transfer platform 2, any surface of the liquid storage platform 3 along the vertical direction is flush with the surface of the sweat transfer platform 2 along the vertical direction. Specifically, it can be either the upper surface or the lower surface.

[0037] When the height of the liquid storage platform 3 is less than the height of the sweat transfer platform 2, neither of the two surfaces of the liquid storage platform 3 in the vertical direction is level. That is, the upper and lower surfaces of the liquid storage platform 3 and the sweat transfer platform 2 are not level.

[0038] When the height of the liquid storage platform 3 is the same as the height of the sweat transfer platform 2, the upper surface of the liquid storage platform 3 is level with the upper surface of the sweat transfer platform 2.

[0039] Preferably, the height of the liquid storage platform 3 is the same as the height of the sweat transfer platform 2.

[0040] In some embodiments of the present invention, the length of the microchannel 21 is 4-6 mm, preferably 5 mm; the microchannel 21 extends horizontally through the sweat transmission platform 2 and has an opening on its upper surface.

[0041] In some embodiments of the present invention, the length of the flow guiding channel 31 is 6-8 mm; preferably, the length of the flow guiding channel 31 is 7.5 mm; the diameter of the liquid storage tank 32 is 2-3 mm, and the height of the liquid storage tank 32 is 290-310 μm. The flow guiding channel 31 extends horizontally through the liquid storage platform 3 and has an opening on its upper surface.

[0042] In some embodiments of the present invention, the lower surface of the flow channel 31 is coplanar with the lower surface of the microchannel 21.

[0043] In some embodiments of the present invention, the longitudinal section of the flow guiding channel 31 is the same as that of the microchannel 21, including but not limited to the shape and size of the longitudinal section. The longitudinal section can be semi-circular or rectangular; specifically, the longitudinal section of the flow guiding channel 31 of the present invention is rectangular.

[0044] In some embodiments of the present invention, the microchannel 21 and the flow channel 31 correspond one-to-one.

[0045] In some embodiments of the present invention, the number of microchannels 21 is not limited. Specifically, the number of microchannels 21 is 8-20; it can be 8-15 or 15-25. The plurality of microchannels 21 are arranged circumferentially at uniform intervals around the central axis of the sweat transfer platform 2.

[0046] In some embodiments of the present invention, the sweat transfer platform 2 is made of a hydrogel material; the present invention does not have a particular limitation on the hydrogel material, and any hydrogel material commonly used by those skilled in the art can be used. Specifically, the hydrogel material is selected from one or more of polyvinyl alcohol hydrogel or polyethylene oxide hydrogel.

[0047] In some embodiments of the present invention, the liquid storage platform 3 is made of polydimethylsiloxane. The present invention does not specifically limit the polydimethylsiloxane material; any polydimethylsiloxane commonly used by those skilled in the art can be used. Specifically, the polydimethylsiloxane can be commercially available or prepared using existing methods. The preparation of the liquid storage platform 3 is common knowledge and can be described using existing knowledge; therefore, it will not be described in detail here. The manufacturer of the polydimethylsiloxane is Dow Corning 184.

[0048] In some embodiments of the present invention, the liquid storage platform 3 is hydrophilically treated. The present invention does not specifically limit the method of hydrophilic treatment; any hydrophilic treatment method commonly used by those skilled in the art can be used. Specifically, the present invention uses plasma treatment to hydrophilically treat the liquid storage platform 3. The present invention does not specifically limit the conditions for plasma treatment; any conditions commonly used in the art can be used.

[0049] In some embodiments of the present invention, a connecting hole 33 is provided at the center of the liquid storage tank 32; the connecting hole 33 penetrates the liquid storage platform 3 axially.

[0050] In some embodiments of the present invention, the diameter of the connecting hole 33 is 450-550 μm; specifically, the diameter of the connecting hole 33 is 500 μm.

[0051] In some embodiments of the present invention, the substrate layer 1 is made of polydimethylsiloxane. The material requirements for the substrate layer 1 are the same as those for the liquid storage platform 3, and will not be described in detail here.

[0052] In some embodiments of the present invention, a sweat-inducing component 4 is further included on the substrate layer 1. The sweat-inducing component 4 includes a sweat-inducing electrode, which comprises an anode 42 and a cathode 41. The anode 42 is disposed on the side of the sweat transmission platform 2 near the substrate layer 1. The cathode 41 includes an electrode layer disposed on the substrate layer 1 and a gel layer disposed on the electrode layer. The sweat transmission platform 2 material includes a sweat-inducing substance. When the sweat-inducing electrode is energized, the sweat-inducing substance in the sweat transmission platform 2 enters the skin to generate sweat. The shape of the anode 42 is the same as the shape of the surface of the sweat transmission platform 2 away from the microchannel 21. The sweat-inducing component 4 facilitates increased perspiration to enable real-time detection of sweat.

[0053] In some embodiments of the present invention, the sweat transmission platform 2, the anode 42, and the substrate layer 1 are formed by stacking and pressing.

[0054] In some embodiments of the present invention, the present invention does not specifically limit the sweat-inducing substance, and any sweat-inducing substance commonly used by those skilled in the art can be used. Specifically, the sweat-inducing substance is carbacholine; the preparation of the sweat transmission platform 2 is common knowledge and can be described using existing knowledge, and will not be described in detail here.

[0055] In some embodiments of the present invention, based on the sweat transmission platform 2, the mass percentage of the sweat-inducing substance in the sweat transmission platform 2 is 4-6%.

[0056] In some embodiments of the present invention, the material requirements for the gel layer are the same as those for the sweat transfer platform 2, and will not be specifically described here. The thickness of the gel layer is 2 mm.

[0057] In some embodiments of the present invention, the sweat-inducing component 4 further includes an induced sweating circuit unit, wherein the anode 42 and cathode 41 are electrically connected to the induced sweating circuit unit. The induced sweating circuit unit is known in the art and can be operated using methods known in the art. Specifically, the electroosmotic circuit in the induced sweating circuit unit of the present invention uses a conventional circuit board made in the laboratory. The cathode 41 and anode 42 are known in the art and can be operated using methods known in the art. The induced sweating circuit unit is a flexible printed circuit board, abbreviated as FPCB, used to supply power to the sweat-inducing electrodes.

[0058] A second aspect of the present invention is to provide an integrated sweat collection and detection system, including the sweat collection device as described above.

[0059] In some embodiments of the present invention, the storage tank 32 is provided with a three-electrode system 5 for real-time detection of biomarkers in sweat within the storage tank 32. The three-electrode system 5 includes a working electrode 51, a reference electrode 53, and a counter electrode 52. The working electrode 51, the reference electrode 53, and the counter electrode 52 are respectively connected to an external device via leads for real-time detection of sweat within the storage tank 32.

[0060] Sweat contains abundant biomarkers (such as electrolytes (Na+)). + K + Sweat secretion is a continuous physiological process, and in-situ real-time detection can achieve long-term, uninterrupted tracking of physiological indicators (such as changes in electrolyte and metabolite concentrations). It can also detect multiple indicators simultaneously, making up for the shortcomings of traditional single-point detection in reflecting dynamic changes. This invention collects sweat into a storage tank 32 through a sweat collection device. The sweat in the storage tank 32 is then detected in-situ by a three-electrode system 5, resulting in higher detection efficiency.

[0061] In some embodiments of the present invention, both the sweat transfer platform 2 and the liquid storage platform 3 are prepared by 3D printing. 3D printing is common knowledge in the art, and conventional methods in the art are used for preparation. Specifically, the preparation method of the liquid storage platform 3 is as follows: a 3D model is constructed using SolidWorks to prepare a mold; the mold is printed using a photopolymer 3D printer with resin material; after cleaning, the mold is placed under ultraviolet light for curing for 30 minutes. PDMS is mixed with prepolymer and curing agent in a 10:1 ratio, poured into the mold, and cured in a 60°C high-temperature drying oven. The cured device is then peeled off from the mold. The preparation method of the sweat transfer platform 2 includes the following steps: a 3D model is constructed using SolidWorks to prepare a mold; the mold is printed using a photopolymer 3D printer with resin material; after cleaning, the mold is placed under ultraviolet light for curing for 30 minutes. Mix 5% hydrogel, 5% perspiration agent and water, stir at 85℃ for 1 hour, then pour into a mold, place at room temperature for 30 minutes, then cure at -20℃ for 2 hours, then take it out and place at room temperature for 30 minutes, then cure at -20℃ for 2 hours, and then peel the cured device off the mold.

[0062] In some embodiments of the present invention, the external device may be a flexible printed circuit board (FPCB), the leads are conductive wires, and the FPCB applies voltage to the working electrode 51 through the leads, and then collects the electrochemical response signal generated by the three-electrode system 5.

[0063] A third aspect of the present invention provides a method of using an integrated sweat collection and detection system, comprising the following steps:

[0064] (1) The surface of the sweat transfer platform 2 containing microchannels 21 is attached to the skin;

[0065] (2) When the sweat-inducing electrode is energized, an electric current is formed on the skin surface between the cathode 41 and the anode 42, and the sweat-inducing substance in the sweat transmission platform 2 enters the skin to produce sweat.

[0066] (3) Sweat enters the microchannel 21 through the surface of the sweat transfer platform 2, and then flows into the guide channel 31 along the microchannel 21 and enters the liquid storage tank 32;

[0067] (4) The three-electrode system 5 detects biomarkers in sweat in the storage tank 32 in real time.

[0068] In some embodiments of the present invention, the current in step (2) is 0.4-1 mA / cm. 2 And any value between them, or the range between any two values. For example, it could be 0.4-0.7 mA / cm. 2 0.7-1 mA / cm 2 .

[0069] In some embodiments of the present invention, the time for sweat production in step (2) is 6-8 minutes.

[0070] The fourth aspect of the present invention provides the application of the sweat collection device and / or the sweat collection-detection integrated system as described above in sweat detection.

[0071] The present invention will be further illustrated by the following examples, but these examples do not limit the scope of the invention.

[0072] When numerical ranges are given in the embodiments, it should be understood that, unless otherwise stated in the present invention, both endpoints of each numerical range and any value between the two endpoints may be selected. Unless otherwise defined, all technical and scientific terms used in this invention have the same meaning as commonly understood by one of ordinary skill in the art. Where specific conditions are not specified in the embodiments, conventional conditions or conditions recommended by the manufacturer shall apply. All reagents or instruments whose manufacturers are not specified are conventional products that can be purchased commercially. In addition to the specific methods, equipment, and materials used in the embodiments, based on the knowledge of the prior art possessed by one of ordinary skill in the art and the description of this invention, any prior art methods, equipment, and materials similar to or equivalent to those described, used, and materials in the embodiments of this invention may be used to implement this invention.

[0073] Please see Figure 1-5 It should be noted that the illustrations provided in this embodiment are only schematic representations of the basic concept of the present invention. Therefore, the drawings only show the components related to the present invention and are not drawn according to the actual number, shape and size of the components in the actual implementation. In the actual implementation, the form, quantity and proportion of each component can be arbitrarily changed, and the layout of the components may also be more complex.

[0074] See Figure 1-5 This invention provides an integrated sweat collection and detection system and its usage method, as well as a sweat collection device.

[0075] See Figure 1-4A sweat collection device includes a substrate layer 1 and a sweat collection assembly disposed on the substrate layer 1 for collecting sweat. The sweat collection assembly includes a sweat transfer platform 2 and a storage platform 3 coaxially nested from the outside to the inside. The sweat transfer platform 2 is provided with multiple microchannels 21, and the storage platform 3 is provided with a guide channel 31 and a storage tank 32. The guide channel 31 corresponds to and communicates with the microchannels 21. The surface of the sweat transfer platform 2 is close to the skin area to be tested. Sweat flows along the surface of the sweat transfer platform 2 into the microchannels 21, and then flows along the guide channel 31 into the storage tank 32. The arrangement of multiple microchannels 21 facilitates the aggregation of sweat, making it easier to collect sweat into the storage tank 32, thereby reducing the situation where sweat is distributed in various positions on the sweat transfer platform 2 and cannot be aggregated into a sweat flow, which could lead to unstable sample volume.

[0076] In some embodiments of the present invention, see Figure 1-3 The sweat transfer platform 2 and the liquid storage platform 3 are connected in a detachable manner. The sweat transfer platform 2 is fitted over the liquid storage platform 3, so that the inner surface of the sweat transfer platform 2 is in contact with the outer surface of the liquid storage platform 3.

[0077] In some embodiments of the present invention, see Figure 1-3 The cross-section of the sweat transfer platform 2 can be annular or square, preferably annular. The cross-section of the liquid storage tank 32 is circular, and the portion of the liquid storage platform 3 with the guide channel 31 is annular. The height of the sweat transfer platform 2 is 2 mm. The height of the liquid storage platform 3 can be less than or equal to the height of the sweat transfer platform 2. Preferably, the height of the liquid storage platform 3 is the same as that of the sweat transfer platform 2, that is, the surface of the sweat transfer platform 2 along the vertical direction is coplanar with the surface of the liquid storage platform 3. The length of the microchannel 21 is 5 mm, and the longitudinal section of the guide channel 31 is exactly the same as the longitudinal section of the microchannel 21, with its lower surface coplanar. The length of the guide channel 31 is 7.5 mm, the depth of the liquid storage tank 32 is 300 μm, and the diameter of the liquid storage tank 32 is 2.5 mm.

[0078] In some embodiments of the present invention, see Figure 1 and Figure 4 The substrate layer 1 is made of polydimethylsiloxane, the sweat transfer platform 2 is made of hydrogel material, and the hydrogel material is polyvinyl alcohol hydrogel; the liquid storage platform 3 is made of polydimethylsiloxane.

[0079] In some embodiments of the present invention, see Figure 1The multiple microchannels 21 are arranged circumferentially at uniform intervals around the central axis of the sweat transfer platform 2. The number of microchannels 21 can be set to 8-20, preferably 15.

[0080] In some embodiments of the present invention, see Figure 1 The liquid storage platform 3 is hydrophilic to make the flow channel 31 and the liquid storage tank 32 hydrophilic. The microchannel 21, the flow channel 31 and the liquid storage tank 32 form a gradient humidification mechanism, which facilitates more stable collection of sweat. Specifically, the present invention uses a plasma treatment method to hydrophilize the liquid storage platform 3.

[0081] In some embodiments of the present invention, see Figure 1 To balance the pressure within the storage tank 32, a connecting hole 33 is provided at the center of the storage tank 32; the connecting hole 33 extends axially through the storage platform 3. When the storage tank 32 is filled with sweat, some of the sweat is discharged through the connecting hole 33. The radius of the connecting hole 33 is 500 μm.

[0082] In some embodiments of the present invention, see Figure 1 and Figure 4 To further increase the sweat collection speed, the sweat collection device also includes a sweat-inducing component 4 disposed on the substrate layer 1. The sweat-inducing component 4 includes a sweat-inducing electrode, which comprises an anode 42 and a cathode 41. The anode 42 is disposed on the side of the sweat transmission platform 2 near the substrate layer 1, and its shape is identical to the shape of the surface of the sweat transmission platform 2 away from the microchannel 21. The cathode 41 includes an electrode layer disposed on the substrate layer 1 and a gel layer disposed on the electrode layer. The gel layer has a thickness of 2 mm. The gel layer on the cathode 41 forms a circuit with the anode 42. The sweat transmission platform 2 material includes a sweat-inducing substance. When the sweat-inducing electrode is energized, the sweat-inducing substance in the sweat transmission platform 2 enters the skin to produce sweat. The sweat-inducing substance is carbacholine, and the amount of sweat-inducing substance added to the sweat transmission platform 2 is 5% of the mass of the sweat transmission platform 2. After the sweat-inducing electrode is energized, an electric current is formed on the skin between the anode 42 and the cathode 41, which stimulates the sweat transmission platform 2 to release the sweat-inducing substance. The sweat-inducing substance enters the skin through electrodialysis, combines with the sweat glands to produce sweat, and the produced sweat enters the microchannel 21 through the sweat transmission platform 2. After the sweat accumulates in the microchannel 21, it flows into the liquid storage tank 32 through the guide channel 31.

[0083] In some embodiments of the present invention, see Figure 1 and Figure 4 The sweat-inducing component 4 also includes an induced excretion circuit unit. The electroosmotic circuit in this induced excretion circuit unit uses a conventionally manufactured circuit board. The induced excretion circuit unit provides a current of 0.4 mA / cm². 2 .

[0084] See Figure 4 This embodiment also provides a sweat collection-detection integrated system, including the above-mentioned sweat collection device and a three-electrode system 5 for real-time detection of biomarkers in sweat within the sweat collection device. The sweat excretion component 4 stimulates sweat excretion to meet the sweat volume requirements. After the excreted sweat is collected by the sweat collection component, it is detected in situ by the three-electrode system 5, which facilitates more accurate and convenient detection of biomarkers in sweat.

[0085] In some embodiments of the present invention, see Figure 1 and Figure 4 The three-electrode system 5 is set in the liquid storage tank 32 to facilitate real-time detection of sweat in the liquid storage tank 32. The three-electrode system 5 includes a working electrode 51, a reference electrode 53, and a counter electrode 52. The working electrode 51, the reference electrode 53, and the counter electrode 52 pass through the liquid storage platform 3 and are respectively connected to external devices through leads for real-time detection of sweat in the liquid storage tank 32.

[0086] This embodiment also provides a method for using the integrated sweat collection and detection system, including the following steps:

[0087] (1) The surface of the sweat transfer platform 2 containing microchannels 21 is attached to the skin;

[0088] (2) When the sweat-inducing electrode is energized, an electric current is formed on the skin surface between the cathode 41 and the anode 42, and the sweat-inducing substance in the sweat transmission platform 2 enters the skin to produce sweat.

[0089] (3) Sweat enters the microchannel 21 through the surface of the sweat transfer platform 2, and then flows into the guide channel 31 along the microchannel 21 and enters the liquid storage tank 32;

[0090] (4) The three-electrode system 5 detects biomarkers in sweat in the storage tank 32 in real time.

[0091] The sweat collection-detection integrated system of the present invention is prepared by first 3D printing molds for the sweat transfer platform 2 and the storage platform 3, respectively. Then, the sweat transfer platform 2 and the storage platform 3 are printed. The storage platform 3 is subjected to plasma treatment. After treatment, the working electrode 51, reference electrode 53, and counter electrode 52 are nested into the storage tank 32 for subsequent contact with the sweat in the storage tank 32 for detection. The system is then connected to an external device via leads. Next, the storage platform 3 is nested into the annulus of the sweat transfer platform 2, connecting the microchannel 21 to the flow channel 31. Finally, the sweat transfer platform 2 is positioned away from the surface of the microchannel 21, the anode 42, and the substrate layer. The gel layer is then bonded to the cathode 41 layer, and the cathode 41 layer is then bonded to the substrate layer 1, connecting the sweat-inducing electrode to the excretion-promoting circuit unit to complete the assembly of the sweat collection-detection integrated system. The assembled sweat collection-detection integrated system is then bonded to the skin, so that the surface containing the microchannel 21 is in contact with the skin. The sweat-inducing electrode is then energized through the excretion-promoting circuit unit, allowing the sweat-inducing substance in the sweat transmission platform 2 to enter the skin and combine with the sweat glands to form sweat. The sweat collects in the microchannel 21 and then flows along the guide channel 31 into the liquid storage tank 32, where it contacts the three-electrode system 5, thereby achieving real-time detection of sweat.

[0092] See Figure 5 This demonstrates the enrichment effect of sweat on the storage platform 3 and the guiding effect on the microchannel 21. The above embodiments are merely illustrative of the principles and effects of the present invention and are not intended to limit the invention. Any person skilled in the art can modify or alter the above embodiments without departing from the spirit and scope of the invention. Therefore, all equivalent modifications or alterations made by those skilled in the art without departing from the spirit and technical concept disclosed in this invention should still be covered by the claims of this invention.

Claims

1. A sweat collection device, characterized in that, The collection device includes a substrate layer (1) for covering and adhering to the skin and a sweat collection component disposed on the substrate layer (1). The sweat collection component includes a sweat transmission platform (2) and a liquid storage platform (3) coaxially nested from the outside to the inside. The sweat transmission platform (2) and the liquid storage platform (3) are detachably connected. The liquid storage platform (3) has a liquid storage tank (32) coaxially formed. The surface of the sweat transmission platform (2) facing the skin has multiple microchannels (21). The surface of the liquid storage platform (3) has a guide channel (31) that communicates with and corresponds to the microchannels (21). Sweat flows into the liquid storage tank (32) along the microchannels (21) and the guide channel (31).

2. The sweat collection device according to claim 1, characterized in that: It also includes one or more of the following features: S1) The multiple microchannels (21) are arranged circumferentially at uniform intervals around the central axis of the sweat transfer platform (2); preferably, there are 8-20 microchannels (21); S2) The sweat transfer platform (2) is made of hydrogel material; preferably, the hydrogel material is selected from one or more of polyvinyl alcohol hydrogel or polyethylene oxide hydrogel; S3) The liquid storage platform (3) is made of polydimethylsiloxane material; preferably, the liquid storage platform (3) is hydrophilic. S4) The length of the flow channel (31) is 6-8 mm; preferably, the length is 7.5 mm; S5) A connecting hole (33) is provided in the center of the liquid storage tank (32); the connecting hole (33) penetrates the liquid storage platform (3) along the axial direction; S6) The depth of the liquid storage tank (32) is 290-310μm, and the diameter of the liquid storage tank (32) is 2-3mm; S7) The longitudinal section of the microchannel (21) is exactly the same as the longitudinal section of the guide channel (31) and the lower surface is coplanar; the length of the microchannel (21) is 4-6mm; The height of the sweat transfer platform (2) described in S8) is 1.9-2.1 mm; S9) The height of the liquid storage platform (3) is the same as the height of the sweat transfer platform (2); The substrate layer (1) described in S10) is made of polydimethylsiloxane material.

3. The sweat collection device according to claim 1, characterized in that: It also includes a sweat-inducing component (4) disposed on the substrate layer (1). The sweat-inducing component (4) includes a sweat-inducing electrode, which includes an anode (42) and a cathode (41). The anode (42) is disposed on the side of the sweat transmission platform (2) close to the substrate layer (1). The cathode (41) includes an electrode layer disposed on the substrate layer (1) and a gel layer disposed on the electrode layer. The sweat transmission platform (2) material includes a sweat-inducing substance. When the sweat-inducing electrode is energized, the sweat-inducing substance in the sweat transmission platform (2) enters the skin to produce sweat.

4. The sweat collection device according to claim 3, characterized in that: The diaphoretic substance is carbacholine; and / or, based on the sweat transmission platform (2), the mass percentage of the diaphoretic substance in the sweat transmission platform (2) is 4-6%.

5. The sweat collection device according to claim 3, characterized in that: The sweat-promoting component (4) further includes a sweat-promoting circuit unit, wherein the anode (42) and cathode (41) are electrically connected to the sweat-promoting circuit unit.

6. An integrated sweat collection and detection system, characterized in that: Includes the sweat collection device as described in any one of claims 1-5.

7. The sweat collection-detection integrated system according to claim 6, characterized in that: The storage tank (32) is equipped with a three-electrode system (5) for real-time detection of biomarkers in sweat within the storage tank (32). The three-electrode system (5) includes a working electrode (51), a reference electrode (53), and a counter electrode (52). The working electrode (51), the reference electrode (53), and the counter electrode (52) are connected to external devices via leads for real-time detection of sweat within the storage tank (32).

8. A method of using the sweat collection-detection integrated system as described in any one of claims 6-7, characterized in that: Includes the following steps: (1) The surface of the sweat transfer platform (2) containing microchannels (21) is attached to the skin; (2) When the sweat-inducing electrode is energized, an electric current is formed on the skin surface between the cathode (41) and the anode (42), and the sweat-inducing substance in the sweat transmission platform (2) enters the skin to produce sweat. (3) Sweat enters the microchannel (21) through the surface of the sweat transfer platform (2), and then flows into the guide channel (31) along the microchannel (21) and enters the liquid storage tank (32); (4) Three-electrode system (5) Real-time detection of biomarkers in sweat in the storage tank (32).

9. The method of use according to claim 8, characterized in that: It also includes one or more of the following features: 21) The current in step (2) is 0.4-1 mA / cm 2 ; 22) The time for sweat to be produced in step (2) is 6-8 minutes.

10. The application of the sweat collection device as described in any one of claims 1-5 and / or the sweat collection-detection integrated system as described in any one of claims 6-7 in sweat detection.

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