Argania tree kernel oil and application thereof
By processing Argan kernel oil through fermentation to form a nano-microemulsion, the dispersibility and stability issues of Argan kernel oil in water-based systems are solved, thereby improving its performance in shampoos and the utilization rate of active ingredients.
Patent Information
- Application Number
- CN202511396163.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-09-28
- Publication Date
- 2025-12-19
AI Technical Summary
Argan kernel oil exhibits poor dispersibility and stability in water-based systems, resulting in low utilization of active ingredients. Furthermore, it is prone to demulsification and precipitation in surfactant systems, affecting the product's performance and stability.
Argan kernel oil was processed by fermentation, and nano-microemulsions were formed through ultrasonic pre-emulsification, compound enzyme treatment, plant lactic acid bacteria fermentation, and natural emulsifiers to improve its stability and dispersibility in aqueous systems.
It significantly improves the stability and dispersibility of Argan kernel oil in aqueous systems, enhances its storage stability and sensory consistency in shampoos, and improves the permeability and antioxidant capacity of active ingredients.
Smart Images
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Abstract
Description
TECHNICAL FIELD
[0001] The present application relates to the technical field of daily chemicals, in particular to argania spinosa kernel oil and application thereof. BACKGROUND
[0002] Argania spinosa kernel oil, also known as argan oil, is a plant oil extracted from the seeds of argan trees native to Morocco, which is rich in active ingredients such as monounsaturated fatty acids (such as omega-9), phytosterols, polyphenols, squalene and vitamin E, and has excellent antioxidant, moisturizing, repairing and nourishing properties. In recent years, argan oil has been widely used in the fields of cosmetics, skin care products, hair care products, etc., and has shown good effects in hair repair, dry and frizzy hair improvement and scalp care.
[0003] However, the traditional argan oil still has the following technical problems in the actual application of daily chemical products: (1) argan oil is a strong hydrophobic oil, which is difficult to disperse uniformly in water-based systems such as shampoo, and is prone to layering and oil separation, affecting the appearance and use of the product; (2) argan oil has a large molecular weight and poor skin permeability, and the utilization rate of its active ingredients is limited; (3) in the presence of surfactants, argan oil is prone to demulsification and separation, resulting in insufficient stability; (4) the antioxidant capacity of conventional cold-pressed argan oil has not been fully utilized, and some phenolic substances are still in the form of phenolic glycoside conjugates, with low bioavailability.
[0004] Therefore, it is urgent to develop a new argania spinosa kernel oil processing method that can improve its stability and dispersibility in aqueous systems, enhance the release and penetration of its active ingredients, and improve its compatibility and performance in cleaning daily chemical products such as shampoo. SUMMARY
[0005] To solve the above technical problems, the present application provides an argania spinosa kernel oil prepared by fermentation, and the specific steps are as follows:
[0006] (1) Mix the argania spinosa kernel oil obtained by cold pressing with sterile deionized water at a volume ratio of 1:1, and perform ultrasonic pre-emulsification treatment at a frequency of 20 kHz and a power of 100-300 W, with a treatment time of 10 minutes;
[0007] (2) Add a composite enzyme preparation to the mixture obtained in step (1);
[0008] (3) Adjust the pH value of the reaction system to 5.0-5.5, control the temperature at 35-40℃, and react for 6-12 hours under the condition of stirring speed of 200 rpm;
[0009] (4) after the enzymatic hydrolysis is completed, the reaction system is heated to 65-70℃ for 10 minutes and then is allowed to stand to separate the phases, and the upper oil phase is taken and is recorded as "enzymatically hydrolyzed argan oil";
[0010] (5) the "enzymatically hydrolyzed argan oil" is mixed with a culture solution, and the volume ratio of the oil to the culture solution is 1:1;
[0011] (6) the plant lactic acid bacteria strain is inoculated into the mixture of the oil and the culture solution, the fermentation temperature is controlled to be 28-32℃, the oscillation rate is 150 rpm, and the fermentation time is 20-30 hours; after the fermentation is completed, sterile filtration treatment is performed by using a filter membrane with a pore size of 0.22 μm, and the argan kernel oil modified by enzymatic fermentation is obtained;
[0012] (7) the argan kernel oil modified by enzymatic fermentation is mixed with a natural emulsifier;
[0013] (8) the mixture obtained in step (7) is added into deionized water (the volume ratio is 1:5) to perform pre-emulsification treatment, the stirring rate is 500-800 rpm, and the stirring time is 10 minutes; then a high-shear homogenizer is used to perform treatment at a rotation speed of ≥10,000 rpm for 5 minutes, and further homogenization treatment is performed at a pressure of 100-150 MPa for 2 cycles, and the argan kernel oil modified by enzymatic fermentation is obtained.
[0014] As an embodiment of the present application, the composite enzyme preparation in step (2) comprises lipase, cellulase and β-glucosidase, and the mass ratio of the three is (1-2):(0.5-1):(0.2-0.5), and the total amount of the enzyme added is 0.5%-2% of the mass of the argan oil.
[0015] As an embodiment of the present application, the culture solution in step (5) comprises glucose, glycerol, yeast extract and sodium chloride, and the mass ratio is 2:0.5:1:0.3.
[0016] As an embodiment of the present application, the strain in step (6) is Lactobacillus plantarum, and the inoculation amount is 3%-5% of the volume of the fermentation system.
[0017] As an embodiment of the present application, the natural emulsifier in step (7) comprises lecithin, polyglycerol-6 didecanate and caprylic / capric glyceride, and the mass ratio of the three is (1-2):(2-4):(1-2); and the mass ratio of the argan kernel oil modified by enzymatic fermentation to the natural emulsifier is 1:2.
[0018] As an embodiment of the present application, the argan kernel oil is applied to the field of daily chemicals.
[0019] A hair-washing product comprising said argan kernel oil.
[0020] As an embodiment of the present application, the preparation raw material comprises: a solvent, a surfactant, a moisturizer, an antioxidant, a preservative, and a conditioning agent.
[0021] A hair-care product comprising said argan kernel oil.
[0022] A skin-care product comprising said argan kernel oil.
[0023] With the above technical solution, the present application has the following beneficial effects:
[0024] The argan kernel oil of the present application is treated by hydrolysis with complex enzymes (such as lipase, phenol glycoside enzyme, etc.), effectively releasing the active ingredients in the argan glycerol in the form of phenol glycoside, improving its antioxidant capacity and biological activity; the oil phase microstructure is regulated by plant lactic acid bacteria fermentation, forming natural surface active metabolites at the oil / water interface, so that the argan glycerol has better skin affinity and permeability; with the help of natural emulsifiers, a nano microemulsion with a particle size of 20-80 nm is formed, significantly improving the stability, transparency and dispersibility of the argan glycerol in the aqueous phase system, and the adaptability is good; the modified argan glycerol will not separate oil or flocculate in the cleaning system of sodium laureth sulfate and betaine, significantly improving the storage stability and sensory consistency of the shampoo. DETAILED DESCRIPTION
[0025] The present application will be further explained and described below in conjunction with specific embodiments.
[0026] Example 1
[0027] This embodiment provides an argan kernel oil prepared by fermentation, and the specific steps are as follows:
[0028] (1) The argania spinosa kernel oil obtained by cold pressing method is mixed with sterile deionized water at a volume ratio of 1:1, and ultrasonic pre-emulsification treatment is carried out at a frequency of 20 kHz and a power of 100-300 W, with a treatment time of 10 minutes;
[0029] (2) Add a complex enzyme preparation to the mixture obtained in step (1); the complex enzyme preparation in step (2) includes lipase, cellulase and β-glucosidase, and the mass ratio of the three is 1:0.6:0.3, and the total amount of enzyme added is 1.5% of the mass of argan oil.
[0030] (3) Adjust the pH value of the reaction system to 5.0-5.5, control the temperature at 35-40℃, and react for 8 hours under the condition of stirring speed of 200 rpm;
[0031] (4) after the enzymatic hydrolysis is completed, the reaction system is heated to 65-70°C for 10 minutes and then is allowed to stand to separate the phases, and the upper oil phase is taken and is recorded as "enzymatically treated argan oil";
[0032] (5) the "enzymatically treated argan oil" is mixed with a culture solution at a volume ratio of 1:1, the culture solution comprises glucose, glycerol, yeast extract and sodium chloride, and the mass ratio is 2:0.5:1:0.3.
[0033] (6) the mixture of the oil and the culture solution is inoculated with a plant lactic acid bacteria strain, the fermentation temperature is controlled at 28-32°C, the shaking speed is 150 rpm, and the fermentation time is 20-30 hours; after the fermentation is completed, sterile filtration treatment is performed using a filter membrane with a pore size of 0.22 μm to obtain argan kernel oil modified by enzymatic fermentation; the strain in step (6) is Lactobacillus plantarum, and the inoculation amount is 4% of the volume of the fermentation system.
[0034] (7) the argan kernel oil modified by enzymatic fermentation is mixed with a natural emulsifier; the natural emulsifier in step (7) comprises lecithin, polyglycerol-6 didecanate and caprylic / capric glyceride, and the mass ratio of the three is 1:2:1; the mass ratio of the argan kernel oil modified by enzymatic fermentation to the natural emulsifier is 1:2.
[0035] (8) the mixture obtained in step (7) is added to deionized water (at a volume ratio of 1:5) for pre-emulsification treatment, the stirring speed is 600 rpm, and the stirring time is 10 minutes; then a high-shear homogenizer is used for treatment at a speed of ≥10000 rpm for 5 minutes, and further homogenization treatment is performed at a pressure of 100 MPa for 2 cycles to obtain the argan kernel oil modified by enzymatic fermentation.
[0036] Example 2
[0037] The difference between this example and Example 1 is that the argan kernel oil is commercially purchased.
[0038] Example 3
[0039] The difference between this example and Example 1 is that the argan kernel oil in this example is prepared by fermentation, and the specific steps are as follows:
[0040] (1) argan kernel oil obtained by cold pressing is mixed with sterile deionized water at a volume ratio of 1:1, and ultrasonic pre-emulsification treatment is performed at a frequency of 20 kHz and a power of 100-300 W for 10 minutes;
[0041] (2) adding a complex enzyme preparation into the mixture obtained in step (1); the complex enzyme preparation in step (2) comprises lipase, cellulase and β-glucosidase, and the mass ratio of the three is 1:0.6:0.3, and the total amount of the enzyme added is 1.5% of the mass of argan oil.
[0042] (3) adjusting the pH value of the reaction system to 5.0-5.5, controlling the temperature to be 35-40°C, and reacting for 8 hours under the condition that the stirring speed is 200 rpm;
[0043] (4) after the enzymolysis is completed, heating the reaction system to 65-70°C for 10 minutes, then standing and separating the phases, and taking the upper oil phase.
[0044] Example 4
[0045] The difference between this embodiment and Example 1 is that this embodiment provides argan kernel oil prepared by fermentation, and the specific steps are as follows:
[0046] (1) mixing argania spinosa kernel oil obtained by cold pressing method with sterile deionized water according to a volume ratio of 1:1, and performing ultrasonic pre-emulsification treatment under the condition that the frequency is 20 kHz and the power is 100-300 W, and the treatment time is 10 minutes;
[0047] (2) adding a complex enzyme preparation into the mixture obtained in step (1); the complex enzyme preparation in step (2) comprises lipase, cellulase and β-glucosidase, and the mass ratio of the three is 1:0.6:0.3, and the total amount of the enzyme added is 1.5% of the mass of argan oil.
[0048] (3) adjusting the pH value of the reaction system to 5.0-5.5, controlling the temperature to be 35-40°C, and reacting for 8 hours under the condition that the stirring speed is 200 rpm;
[0049] (4) after the enzymolysis is completed, heating the reaction system to 65-70°C for 10 minutes, then standing and separating the phases, and taking the upper oil phase.
[0050] (5) mixing the "enzymolysis-treated argan oil" with a culture solution, and the volume ratio of oil to culture solution is 1:1; the culture solution comprises glucose, glycerol, yeast extract and sodium chloride, and the mass ratio is 2:0.5:1:0.3.
[0051] (6) inoculating the plant lactic acid bacteria strain into the mixture of the above oil and the culture solution, controlling the fermentation temperature to be 28-32℃, the oscillation rate to be 150 rpm, and the fermentation time to be 20-30 hours; after the fermentation is completed, performing sterile filtration treatment by using a filter membrane with a pore size of 0.22 μm, to obtain the argania spinosa kernel oil modified by enzymatic fermentation; the strain in the step (6) is Lactobacillus plantarum, and the inoculation amount is 4% of the volume of the fermentation system. The argania spinosa kernel oil modified by enzymatic fermentation is obtained.
[0052] Lactiplantibacillus paraplantarum
[0053] The above examples 1-4 are applied to the following shampoo formula to obtain application examples 1-4, and the formula is as follows:
[0054] Table 1. Application example formula
[0055]
[0056] The preparation process is as follows:
[0057] In the main pot, add water (A-1), start stirring and heat to 40-45℃. Add glycerol, nicotinamide, allantoin, disodium EDTA in turn, stir until completely dissolved. Cool to 35-40℃, slowly add SLES (A-2), cocamidopropyl betaine (A-3), sodium lauroyl methyl amino acid (A-4), sodium methyl cocoyl taurine (A-5) in turn, stir while adding, make sure that no large amount of foam is generated, and form a uniform mixed system. Mix argania spinosa (B-1) and olive oil PEG-8 ester (B-2) separately to form a uniform solution; slowly drop the oil phase into the main pot while maintaining low speed stirring to form stable emulsion.
[0058] Disperse polyquaternary ammonium salt-10 and guar gum derivatives; stir evenly, continue stirring for more than 10 minutes to avoid gel clumping. Add camellia seed extract, phenoxyethanol, ethylhexyl glycerin, and stir thoroughly.
[0059] Slowly drop citric acid solution (10%) to pH 5.5-6.0; add appropriate amount of sodium chloride (0.5-1.0%) as needed to adjust the viscosity of the system to the target range (such as 2000-5000 mPa·s).
[0060] Application example 5: Place the argania spinosa kernel oil obtained in example 1 in the following system to obtain application example 5.
[0061] Table 2. Formula of application example 5
[0062]
[0063]
[0064] Performance test
[0065] Test 1: Stability evaluation method
[0066] Objective: Put the sample to be tested into a transparent polypropylene container (100 mL) and store it under the following conditions: room temperature (25 ± 2°C), high temperature (40 ± 2°C), refrigeration (4 ± 1°C), high temperature and high humidity (40°C / 75% RH); observe the appearance every 7 days and record the photos, and continuously observe for 28 days.
[0067] Table 3 Judgment index
[0068]
[0069] Table 4 Stability performance test results
[0070]
[0071] Test 2: Efficacy test method
[0072] 2.1 Hair smoothness evaluation
[0073] Test subjects: Select 20 subjects aged 18-45 years old with mild to moderate hair dryness / roughness problems.
[0074] Usage:
[0075] Use a standard amount of sample (2 mL) to wash the hair once, and let it dry naturally; evaluate the smoothness, static electricity, and luster of the hair before and after the trial.
[0076] Table 5 Evaluation method
[0077]
[0078] Table 6 Hair smoothness test results
[0079] Application Examples Smoothness Gloss Value Improvement Rate Static Reduction Rate Application Example 1 4.8 37.80％ 66.10％ Application Example 2 2.4 13.50％ 26.10％ Application Example 3 3.5 27.60％ 47.30％ Application Example 4 3.1 22.20％ 40.40％ Application Example 5 3.0 21.30％ 37.60％
[0080] Post-wash scalp moisturization test
[0081] Test method:
[0082] Use a stratum corneum moisture measuring instrument (such as Cutometer or Corneometer);
[0083] Measure the stratum corneum moisture value of the scalp before washing, 30 minutes and 60 minutes after washing.
[0084] Table 7 Test Standard
[0085] Time Point Moisture Value Improvement Rate (%) 30 minutes ≥ 10% is effective improvement 60 minutes ≥ 5% indicates sustained moisturization
[0086] Table 8 Scalp Moisturizing Test Results
[0087]
[0088] Antioxidant Capacity Test
[0089] Test Method: DPPH Free Radical Scavenging Rate Method
[0090] Take the oil of Examples 1-4, and prepare 0.1 mmol / L DPPH ethanol solution; after 30 minutes of reaction between the sample and DPPH, measure the absorbance change at 517 nm.
[0091] Calculation Formula: (A0-As) / A0*100%
[0092] Wherein: A0 is the absorbance of the blank control, and As is the absorbance after reaction of the sample.
[0093] Table 9 Antioxidant Capacity Test Results
[0094]
[0095]
[0096] Finally, it should be noted that: the above examples are only used to illustrate the technical solutions of the present application, and not to limit them; although the present application has been described in detail with reference to the foregoing examples, those skilled in the art should understand that: it can still modify the technical solutions recorded in the foregoing examples, or make equivalent replacement for part or all of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present application.
Claims
1. An Argan kernel oil, characterized in that, It is prepared by fermentation, and the specific steps are as follows: (1) The kernel oil of Argania spinosa obtained by cold pressing was mixed with sterile deionized water at a volume ratio of 1:1 and subjected to ultrasonic pre-emulsification treatment at a frequency of 20kHz and a power of 100-300W for 10 minutes. (2) Add the compound enzyme preparation to the mixture obtained in step (1); (3) Adjust the pH of the reaction system to 5.0 to 5.5, control the temperature to 35 to 40°C, and react for 6 to 12 hours with a stirring speed of 200 rpm. (4) After the enzymatic hydrolysis is completed, the reaction system is heated to 65-70℃ and kept at that temperature for 10 minutes, and then allowed to stand for phase separation. The upper oil phase is taken for later use and is recorded as "enzymatic hydrolysis of Argan oil". (5) Mix the "enzymatically hydrolyzed argan oil" with the culture medium, and the mixing ratio is 1:1 (volume ratio of oil to culture medium). (6) Plant lactic acid bacteria were inoculated into the mixture of the above oil and culture medium, and the fermentation temperature was controlled at 28-32℃, the shaking rate was 150rpm, and the fermentation time was 20-30 hours. After the fermentation was completed, the mixture was sterile filtered using a filter membrane with a pore size of 0.22μm to obtain enzymatically hydrolyzed fermented modified Argan kernel oil. (7) Mix the above-mentioned enzymatically hydrolyzed and fermented Agan kernel oil with a natural emulsifier; (8) Add the mixture obtained in step (7) to deionized water (volume ratio of 1:5) for pre-emulsification treatment. Stir at 500-800 rpm for 10 minutes. Then use a high shear homogenizer to process at ≥10000 rpm for 5 minutes, and further homogenize at 100-150 MPa. Repeat this process twice to obtain the final product.
2. The Argan kernel oil according to claim 1, characterized in that, The compound enzyme preparation in step (2) includes lipase, cellulase and β-glucosidase, with a mass ratio of (1-2):(0.5-1):(0.2-0.5), and the total amount of enzyme added is 0.5%-2% of the mass of argan oil.
3. The Argan kernel oil according to claim 1, characterized in that, The culture medium in step (5) includes glucose, glycerol, yeast extract and sodium chloride; and the mass ratio is 2:0.5:1:0.
3.
4. The Argan kernel oil according to claim 1, characterized in that, The strain mentioned in step (6) is Lactobacillus plantarum, and the inoculation amount is 3% to 5% of the fermentation system volume.
5. An Argan kernel oil according to claim 1, characterized in that, The natural emulsifier in step (7) includes lecithin, polyglycerol-6 didecanoate and caprylic / capric glyceride, with a mass ratio of (1-2):(2-4):(1-2); the mass ratio of the enzymatically hydrolyzed fermented Argan kernel oil to the natural emulsifier is 1:
2.
6. The Argan kernel oil according to any one of claims 1 to 5, characterized in that, It is used in the daily chemical industry.
7. A shampoo product, characterized in that, It includes the Argan kernel oil as described in any one of claims 1 to 5.
8. A shampoo product according to claim 7, characterized in that, Its raw materials include: solvents, surfactants, humectants, antioxidants, preservatives, and conditioning agents.
9. A hair care product, characterized in that, It includes the Argan kernel oil as described in any one of claims 1 to 5.
10. A skincare product, characterized in that, It includes the Argan kernel oil as described in any one of claims 1 to 5.