Cosmetic composition with moisturizing and soothing effects and preparation method thereof

Abstract

The invention discloses a cosmetic composition with moisturizing and soothing effects and a preparation method thereof. The composition is prepared from the following components in percentage by mass: 70 to 80 percent of lotus water, 1 to 5 percent of zymosan, 0.1 to 1 percent of tremella fuciformis extract, 0.5 to 1.5 percent of beta-glucan, 0.1 to 1 percent of 1, 2-hexanediol, 0.1 to 1 percent of p-hydroxyacetophenone and the balance of butanediol. Wherein the lotus water contains flavonoid components such as quercetin and the like, so that the effects of relieving and maintaining stability are achieved. The zymosan, the tremella fuciformis extract and the beta-glucan contain a large amount of hydroxyl groups, are combined with water molecules through hydrogen-bond interaction and have a synergistic effect, so that a layer of moisturizing film is formed on the surface of skin, and the percutaneous water loss rate is reduced. The composition is scientific in proportion, only necessary solvents and functional auxiliaries are added, the skin can be effectively moistened, the skin barrier can be enhanced, and stability and soothing can be maintained, so that the composition is suitable for preparing various cosmetics.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, specifically relating to a cosmetic composition with moisturizing and soothing effects and its preparation method. Background Technology

[0002] With the improvement of people's living standards and the continuous upgrading of their pursuit of beauty, the cosmetics market is showing a booming development trend. Consumers' demands for the efficacy of cosmetics are also becoming increasingly diversified, with moisturizing and soothing becoming the focus of attention for many consumers.

[0003] As the largest organ in the human body, the health of the skin is closely related to various factors such as the external environment and lifestyle habits. In adverse environments such as dryness, cold, and pollution, the skin is prone to moisture loss and impaired barrier function, leading to discomfort such as dryness, itching, and sensitivity. Therefore, developing cosmetics with good moisturizing and soothing effects is of significant practical importance.

[0004] Currently, there are many types of moisturizing and soothing cosmetics on the market, but some products have problems such as limited efficacy, complex ingredients, and high irritation. Some products add a large amount of chemically synthesized moisturizers in pursuit of short-term moisturizing effects, which may burden the skin with long-term use; while other products have limited soothing ingredients that are difficult to effectively relieve skin sensitivity and other problems. Summary of the Invention

[0005] The purpose of this invention is to provide a cosmetic composition with moisturizing and soothing effects and its preparation method, so as to solve the problems of existing moisturizing and soothing cosmetics, such as single efficacy, complex ingredients, and high irritation. This composition can effectively moisturize the skin, enhance the skin barrier, stabilize and soothe, and has high safety.

[0006] A cosmetic composition with moisturizing and soothing effects comprises the following components in the indicated weight percentages: 70-80% lotus water, 1-5% yeast polysaccharide, 0.1-1% tremella extract, 0.5-1.5% β-glucan, 0.1-1% 1,2-hexanediol, 0.1-1% p-hydroxyacetophenone, and the balance butylene glycol.

[0007] Optionally, the mass percentage of the butanediol is 10-30%.

[0008] Optionally, the lotus water has a mass percentage of 72-78%.

[0009] Optionally, the yeast polysaccharide has a mass percentage of 2-4%.

[0010] Optionally, the mass percentage of the tremella extract is 0.3-0.8%.

[0011] Optionally, the β-glucan has a mass percentage of 0.8-1.2%.

[0012] Optionally, the 1,2-hexanediol has a mass percentage of 0.3-0.8%; the p-hydroxyacetophenone has a mass percentage of 0.3-0.8%.

[0013] Optionally, the tremella extract is prepared by the following method: (1) Enzymatic hydrolysis: Place the chopped pieces of white fungus root into an extraction tank, add deionized water at a material-to-liquid ratio of 1g:10mL-1g:15mL, and stir evenly; then add a compound enzyme at a mass percentage of 0.2-0.5% of the white fungus root, the compound enzyme being a mixture of cellulase and pectinase at a mass ratio of 1:1, adjust the pH value in the extraction tank to 4.5-5.5, and carry out the enzymatic hydrolysis reaction at a temperature of 45-50℃ for 1.5-2h; (2) Ultrasonic-assisted extraction: After the enzymatic reaction is completed, the temperature in the extraction tank is raised to 70-80℃ and kept warm for 10-15 minutes to inactivate the enzyme activity; then ultrasonic-assisted extraction is performed with an ultrasonic power of 300-500W and an ultrasonic time of 30-40 minutes.

[0014] (3) Filtration: After extraction, filter the solution while it is still hot using an 80-100 mesh filter cloth and collect the filtrate; (4) Repeat the process of steps (1)-(3) on the filter residue in step (3), and mix the filtrates obtained from the two filtrations to obtain a mixed filtrate; (5) Place the mixed filtrate into a centrifuge and centrifuge at 3000-4000 r / min for 15-20 min to remove suspended impurities and large molecular particles from the filtrate and obtain the supernatant. (6) Macroporous resin adsorption: Take the supernatant after centrifugation and purify it by adsorption through an AB-8 macroporous resin column; control the sample loading flow rate to 1-2 BV / h, where BV is the column volume. After all the sample has been loaded, rinse the resin column with deionized water at a flow rate of 2-3 BV / h until the eluent is clear to remove unadsorbed impurities; then, elute with an ethanol aqueous solution with a volume concentration of 70-80% at a flow rate of 1-2 BV / h and collect the eluent. (7) Concentration: The collected eluent is placed in a rotary evaporator and concentrated under reduced pressure at 50-60℃ and a vacuum of 0.06-0.08MPa until the volume of the eluent is concentrated to 1 / 5-1 / 10 of the original volume to obtain the concentrated solution. (8) Spray dry the concentrate. The inlet air temperature of the spray dryer is 180-200℃, the outlet air temperature is 80-90℃, and the feed rate is 10-15mL / min. After drying, the tremella extract is obtained.

[0015] A method for preparing the cosmetic composition with moisturizing and soothing effects includes the following steps: S101: Add the lotus water and the butanediol to the reaction vessel and stir until homogeneous to obtain mixed solution A; S102: Add the yeast polysaccharide, the tremella extract, and the β-glucan to the mixed solution A in sequence, and stir at 30-40℃ until completely dissolved to obtain mixed solution B; S103: Add the 1,2-hexanediol and the p-hydroxyacetophenone to the mixed solution B, and continue stirring for 15-25 minutes to obtain the cosmetic composition with moisturizing and soothing effects.

[0016] Optionally, the stirring speed in S101 is 100-200 r / min; the stirring speed in S102 is 300-500 r / min; and the stirring speed in S103 is 200-300 r / min.

[0017] Beneficial effects The composition of this invention uses natural ingredients such as lotus water, yeast polysaccharide, tremella extract, and β-glucan as the main active ingredients, combined with a small amount of solvent and functional additives. The composition is simple, with low irritation, and suitable for various skin types, including sensitive skin. Yeast polysaccharide, tremella extract, and β-glucan contain a large number of hydroxyl groups, which bind water molecules through hydrogen bonding. Furthermore, the three components work synergistically to form a moisturizing film on the skin surface, reducing transepidermal water loss. Detailed Implementation

[0018] An embodiment of the present invention provides a cosmetic composition with moisturizing and soothing effects, comprising the following components in the indicated weight percentages: lotus water 70-80%, yeast polysaccharide 1-5%, tremella fuciformis extract 0.1-1%, β-glucan 0.5-1.5%, 1,2-hexanediol 0.1-1%, p-hydroxyacetophenone 0.1-1%, and the balance butylene glycol. The composition uses natural ingredients such as lotus water, yeast polysaccharide, tremella fuciformis extract, and β-glucan as the main active ingredients, combined with small amounts of solvents and functional additives. The composition is simple, has low irritation, and is suitable for various skin types, including sensitive skin. The flavonoids such as quercetin contained in lotus water have good soothing and stabilizing effects, relieving discomfort symptoms such as skin sensitivity and redness, and enhancing the skin's resistance. Yeast polysaccharide, tremella fuciformis extract, and β-glucan contain a large number of hydroxyl groups, which bind water molecules through hydrogen bonding, and the three work synergistically to form a moisturizing film on the skin surface, reducing transepidermal water loss.

[0019] In some embodiments, the mass percentage of butanediol is 10-30%. For example, the mass percentage of butanediol can be 10%, 15%, 20%, 25%, 30%, etc.

[0020] In some embodiments, the lotus water comprises 72-78% by mass. Within this range, flavonoids such as quercetin in the lotus water can better exert their soothing and stabilizing effects, and their synergistic effect with other components is more significant, thereby improving the overall soothing effect of the composition.

[0021] In some embodiments, the yeast polysaccharide comprises 2-4% by mass. In this case, the yeast polysaccharide can work better with the tremella extract and β-glucan, binding more water molecules through hydrogen bonding, thereby enhancing the composition's ability to form a hydrating film on the skin surface and improving the moisturizing effect.

[0022] In some embodiments, the mass percentage of Tremella fuciformis extract is 0.3-0.8%. This allows the active ingredients in the Tremella fuciformis extract to fully exert their effects, synergistically enhancing with yeast polysaccharides and β-glucan, further improving the moisturizing properties of the composition while also providing better skin gentleness.

[0023] In some embodiments, the mass percentage of β-glucan is 0.8-1.2%. In this case, β-glucan can participate more effectively in the synergistic effect with other moisturizing ingredients, enhance the stability of the skin surface moisturizing film, reduce transepidermal water loss, and make the moisturizing effect more lasting.

[0024] In some embodiments, the mass percentage of 1,2-hexanediol is 0.3-0.8%; the mass percentage of p-hydroxyacetophenone is 0.3-0.8%. 1,2-Hexanediol, as a preservative, effectively inhibits microbial growth within this range, ensuring the stability and safety of the composition without adversely affecting the efficacy of other components. p-Hydroxyacetophenone has certain preservative and antioxidant properties, and within this range, it can synergistically work with 1,2-hexanediol to exert a preservative effect, extending the shelf life of the composition. Simultaneously, its antioxidant properties help protect the skin from free radical damage.

[0025] In some embodiments, the Tremella fuciformis extract is prepared by the following method: (1) Enzymatic hydrolysis: Place the chopped pieces of white fungus root into the extraction tank, add deionized water at a material-to-liquid ratio of 1g:10mL-1g:15mL, and stir evenly; then add a compound enzyme at a mass percentage of 0.2-0.5% of the white fungus root. The compound enzyme is a mixture of cellulase and pectinase at a mass ratio of 1:1. Adjust the pH value in the extraction tank to 4.5-5.5, and carry out the enzymatic hydrolysis reaction at a temperature of 45-50℃ for 1.5-2 hours. (2) Ultrasonic-assisted extraction: After the enzymatic reaction is completed, the temperature in the extraction tank is raised to 70-80℃ and kept warm for 10-15 minutes to inactivate the enzyme activity; then ultrasonic-assisted extraction is performed with an ultrasonic power of 300-500W and an ultrasonic time of 30-40 minutes.

[0026] (3) Filtration: After extraction, filter the solution while it is still hot using an 80-100 mesh filter cloth and collect the filtrate; (4) Repeat the process of steps (1)-(3) on the filter residue in step (3), and mix the filtrates obtained from the two filtrations to obtain a mixed filtrate; (5) Place the mixed filtrate into a centrifuge and centrifuge at 3000-4000 r / min for 15-20 min to remove suspended impurities and large molecular particles from the filtrate and obtain the supernatant. (6) Macroporous resin adsorption: Take the supernatant after centrifugation and purify it by adsorption through an AB-8 macroporous resin column; control the sample loading flow rate to 1-2 BV / h, where BV is the column volume. After all the sample has been loaded, rinse the resin column with deionized water at a flow rate of 2-3 BV / h until the eluent is clear to remove unadsorbed impurities; then, elute with an ethanol aqueous solution with a volume concentration of 70-80% at a flow rate of 1-2 BV / h and collect the eluent. (7) Concentration: The collected eluent is placed in a rotary evaporator and concentrated under reduced pressure at 50-60℃ and a vacuum of 0.06-0.08MPa until the volume of the eluent is concentrated to 1 / 5-1 / 10 of the original volume to obtain the concentrated solution. (8) Spray dry the concentrate. The inlet air temperature of the spray dryer is 180-200℃, the outlet air temperature is 80-90℃, and the feed rate is 10-15mL / min. After drying, the tremella extract is obtained.

[0027] The tremella extract prepared by this method can better retain its active ingredients (such as polysaccharides), and has good water solubility and stability. It can be well compatible with other ingredients in the cosmetic composition of this invention to synergistically exert moisturizing effects.

[0028] Another embodiment of the present invention provides a method for preparing the above-mentioned cosmetic composition with moisturizing and soothing effects, comprising the following steps: S101: Add lotus water and butanediol to the reaction vessel and stir until homogeneous to obtain mixed solution A; S102: Add yeast polysaccharide, tremella extract and β-glucan to mixed solution A in sequence, and stir at 30-40℃ until completely dissolved to obtain mixed solution B; S103: Add 1,2-hexanediol and p-hydroxyacetophenone to mixed solution B, and continue stirring for 15-25 minutes to obtain a cosmetic composition with moisturizing and soothing effects.

[0029] This preparation method is simple to operate. By using a reasonable order of adding materials and stirring conditions, it is possible to ensure that all components are fully mixed and dissolved, resulting in a more stable and uniform composition. Stirring at 30-40℃ is beneficial for the dissolution of components such as yeast polysaccharides, thus improving preparation efficiency and product quality.

[0030] Furthermore, the stirring speed in S101 is 100-200 r / min; the stirring speed in S102 is 300-500 r / min; and the stirring speed in S103 is 200-300 r / min. Using different stirring speeds in different steps effectively avoids incomplete dissolution due to excessively low speed or component damage due to excessively high speed, thus effectively promoting uniform mixing of the substances.

[0031] Another embodiment of the present invention provides a cosmetic product comprising the aforementioned moisturizing and soothing cosmetic composition and excipients. Optionally, the moisturizing and soothing cosmetic composition comprises 0.1-10% by mass in the cosmetic product. Further, when the cosmetic product is a leave-on cosmetic, the moisturizing and soothing cosmetic composition comprises 0.1-10% by mass in the cosmetic product. When the cosmetic product is a rinse-off cosmetic, the moisturizing and soothing cosmetic composition comprises 0.1-3% by mass in the cosmetic product.

[0032] Example 1 In this embodiment, the Tremella fuciformis extract was prepared by the following method: (1) Enzymatic hydrolysis: Place the chopped pieces of white fungus root into the extraction tank, add deionized water at a material-to-liquid ratio of 1g:12mL, and stir evenly; then add a compound enzyme at a mass percentage of 0.3% of the white fungus root. The compound enzyme is a mixture of cellulase and pectinase at a mass ratio of 1:1. Adjust the pH value in the extraction tank to 5, and carry out the enzymatic hydrolysis reaction at a temperature of 48℃ for 1.8h. (2) Ultrasonic-assisted extraction: After the enzymatic reaction is completed, the temperature inside the extraction tank is raised to 75°C and kept warm for 12 minutes to inactivate the enzyme activity; then ultrasonic-assisted extraction is performed with an ultrasonic power of 400W and an ultrasonic time of 35 minutes.

[0033] (3) Filtration: After extraction, filter the solution while it is still hot using a 90-mesh filter cloth and collect the filtrate; (4) Repeat the process of steps (1)-(3) on the filter residue in step (3), and mix the filtrates obtained from the two filtrations to obtain a mixed filtrate; (5) Place the mixed filtrate into a centrifuge and centrifuge at 3500 r / min for 18 min to remove suspended impurities and large molecular particles from the filtrate and obtain the supernatant. (6) Macroporous resin adsorption: Take the supernatant after centrifugation and purify it by adsorption through an AB-8 macroporous resin column; control the sample loading flow rate to 1.5 BV / h, where BV is the column volume. After all the sample has been loaded, rinse the resin column with deionized water at a flow rate of 2.5 BV / h until the eluent is clear to remove unadsorbed impurities; then, elute with an ethanol aqueous solution with a volume concentration of 75% at a flow rate of 1.5 BV / h and collect the eluent. (7) Concentration: The collected eluent is placed in a rotary evaporator and concentrated under reduced pressure at 55°C and 0.07 MPa until the volume of the eluent is concentrated to 1 / 10 of the original volume to obtain the concentrated solution. (8) The concentrate was spray-dried at an inlet air temperature of 190°C and an outlet air temperature of 85°C. The feed rate was 12 mL / min. After drying, the tremella extract was obtained.

[0034] In this embodiment, the composition consists of the following components in the indicated mass percentages: 75% lotus water, 2% yeast polysaccharide, 1% tremella extract, 1% β-glucan, 0.5% 1,2-hexanediol, 0.5% p-hydroxyacetophenone, and the balance being butylene glycol.

[0035] The method for preparing the composition is as follows: S101: Add lotus water and butanediol to the reaction vessel and stir at 150 r / min until homogeneous to obtain mixed solution A; S102: Add yeast polysaccharide, tremella extract and β-glucan to mixed solution A in sequence, and stir at 400 r / min at 35℃ until completely dissolved to obtain mixed solution B; S103: Add 1,2-hexanediol and p-hydroxyacetophenone to mixed solution B, and continue stirring at 250 r / min for 20 min to obtain the composition.

[0036] Example 2 Compared with Example 1, Example 2 differs in that the composition consists of the following components in the following mass percentages: lotus water 72%, yeast polysaccharide 4%, tremella extract 0.3%, β-glucan 0.5%, 1,2-hexanediol 0.3%, p-hydroxyacetophenone 0.3%, and the balance butylene glycol.

[0037] Example 3 Compared with Example 1, Example 3 differs in that the composition consists of the following components in the following mass percentages: lotus water 78%, yeast polysaccharide 4%, tremella extract 0.8%, β-glucan 1.2%, 1,2-hexanediol 0.8%, p-hydroxyacetophenone 0.8%, and the balance butylene glycol.

[0038] Example 4 Compared with Example 1, Example 2 differs in that the composition consists of the following components in the following mass percentages: 80% lotus water, 5% yeast polysaccharide, 1% tremella extract, 1.5% β-glucan, 1% 1,2-hexanediol, 1% p-hydroxyacetophenone, and the balance butylene glycol.

[0039] Comparative Example 1 Compared with Example 1, Comparative Example 1 differs in that the composition consists of the following components in the following mass percentages: 65% lotus water, 0.5% yeast polysaccharide, 1.5% tremella extract, 2% β-glucan, 0.3% 1,2-hexanediol, 0.3% p-hydroxyacetophenone, and the balance being butanediol.

[0040] Comparative Example 2 Compared with Example 1, Comparative Example 2 differs in that the composition consists of the following components in the following mass percentages: 85% lotus water, 6% yeast polysaccharide, 0.05% tremella extract, 0.2% β-glucan, 0.3% 1,2-hexanediol, 0.3% p-hydroxyacetophenone, and the balance butylene glycol.

[0041] Comparative Example 3 Compared with Example 1, Comparative Example 3 differs in that the Tremella fuciformis extract is replaced with the same percentage by mass of β-glucan.

[0042] Comparative Example 4 Compared with Example 1, Comparative Example 4 differs in that β-glucan is replaced with the same percentage of Tremella fuciformis extract by mass.

[0043] Comparative Example 5 Compared with Example 1, Comparative Example 5 differs in that yeast polysaccharide is replaced with the same percentage of Tremella fuciformis extract by mass.

[0044] Test case (1) Skin stratum corneum moisture content test: Thirty healthy volunteers, aged 20-35 years, were selected, with half being male and half female. Before the test, the volunteers sat quietly for 30 minutes in an environment with a temperature of 22±2℃ and a relative humidity of 50±5%. Then, the baseline moisture content of the volunteers' inner forearms was tested using a Corneometer CM825 skin moisture meter. Afterward, the compositions of the examples and comparative examples were applied to different areas of the volunteers' inner forearms, each area being 2cm×2cm, with an application rate of 2mg / cm². The skin stratum corneum moisture content of each area was tested 6 hours after application, and the percentage of the tested value to the baseline moisture content was calculated and averaged. The results are shown in the table below.

[0045] (2) Erythema Index Test: Ninety volunteers with mild facial redness, aged 20-35 years, were selected, with an equal number of men and women. They were randomly divided into 9 groups of 10 people each, with an equal number of men and women. Before the test, the baseline erythema index of the volunteers' faces was measured using a Mexameter MX18 erythema index analyzer. Afterwards, the volunteers applied the compositions of the examples and comparative examples to their faces every morning and evening for 28 consecutive days. The percentage of the tested value relative to the baseline erythema index was calculated, and the average value was taken. The results are shown in the table below.

[0046] (3) Thirty healthy volunteers, aged 20-35 years, were selected, with an equal number of males and females. The compositions of the examples and comparative examples were applied to different areas on the inner forearms of the volunteers, each area measuring 2cm × 2cm, with an application amount of 0.1g. The areas were covered with non-irritating adhesive tape and removed after 24 hours. The skin was observed for irritation reactions such as erythema and edema. The skin irritation reaction was scored according to the skin irritation reaction scoring criteria: 0 points for no reaction, 1 point for mild erythema, 2 points for significant erythema, 3 points for erythema with edema, and 4 points for severe erythema, edema, and blisters. The average irritation score was calculated. The results are shown in the table below.

[0047]

[0048] As can be seen from the table above, the composition in the examples can effectively moisturize the skin, enhance the skin barrier, and stabilize and soothe the skin.

[0049] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

[0050] For those skilled in the art, based on the ideas of this invention, there will be changes in the specific implementation methods and application scope. Therefore, the content of this specification should not be construed as a limitation of this invention.

Claims

1. A cosmetic composition having a moisturizing and soothing effect, characterized by, The composition is composed of the following components in percentage by mass: lotus water 70-80%, yeast polysaccharide 1-5%, tremella extract 0.1-1%, beta-glucan 0.5-1.5%, 1,2-hexanediol 0.1-1%, p-hydroxyacetophenone 0.1-1%, and the rest butanediol.

2. The cosmetic composition having a moisturizing and soothing effect according to claim 1, wherein The mass percentage of the butanediol is 10-30%.

3. The cosmetic composition having a moisturizing and soothing effect according to claim 1, wherein the cosmetic composition is a skin care product. The mass percentage of the lotus water is 72-78%.

4. The cosmetic composition having a moisturizing and soothing effect according to claim 1, wherein the cosmetic composition is a skin care product. The mass percentage of the yeast polysaccharide is 2-4%.

5. The cosmetic composition having a moisturizing and soothing effect according to claim 1, wherein the cosmetic composition is a skin care product. The mass percentage of the tremella extract is 0.3-0.8%.

6. The cosmetic composition having a moisturizing and soothing effect according to claim 1, wherein The mass percentage of the beta-glucan is 0.8-1.2%.

7. The cosmetic composition having a moisturizing and soothing effect according to claim 1, wherein the cosmetic composition is a skin care product. The mass percentage of the 1,2-hexanediol is 0.3-0.8%; the mass percentage of the p-hydroxyacetophenone is 0.3-0.8%.

8. The cosmetic composition having a moisturizing and soothing effect according to any one of claims 1 to 7, wherein The tremella extract is prepared by the following method: (1) Enzymatic treatment: cut the tremella roots into small pieces and put them into an extraction tank, add deionized water according to the solid-liquid ratio of 1g:10mL-1g:15mL, and stir uniformly; then add a composite enzyme accounting for 0.2-0.5% of the mass percentage of the tremella roots, the composite enzyme is composed of cellulase and pectinase mixed in a mass ratio of 1:1, adjust the pH value in the extraction tank to 4.5-5.5, and carry out enzymatic reaction at a temperature of 45-50℃ for 1.5-2h; (2) Ultrasonic-assisted extraction: after the enzymatic reaction is completed, the temperature in the extraction tank is raised to 70-80℃ for 10-15min to inactivate the enzyme activity; then ultrasonic-assisted extraction is carried out, the ultrasonic power is 300-500W, and the ultrasonic time is 30-40min. (3) Filtration: after the extraction is completed, hot filtration is carried out with filter cloth of 80-100 mesh to collect the filtrate; (4) The filter residue in step (3) is repeatedly treated by steps (1)-(3), and the filtrates obtained by two times of filtration are mixed to obtain a mixed filtrate; (5) The mixed filtrate is placed in a centrifuge, and centrifuged at a speed of 3000-4000r / min for 15-20min to remove suspended impurities and macromolecular particles in the filtrate to obtain a supernatant; (6) Macroporous resin adsorption: take the supernatant after centrifugation, and carry out adsorption purification through an AB-8 type macroporous resin column; control the sample loading speed to be 1-2BV / h, BV is the column volume, after the sample is completely loaded, wash the resin column with deionized water at a flow rate of 2-3BV / h until the effluent is clear to remove unabsorbed impurities; then, elute with 70-80% ethanol aqueous solution at a flow rate of 1-2BV / h, and collect the eluate; (7) Concentration: place the collected eluate into a rotary evaporator, and carry out vacuum concentration under the conditions of 50-60℃ and vacuum degree of 0.06-0.08MPa until the volume of the eluate is concentrated to 1 / 5-1 / 10 of the original volume to obtain a concentrated solution; (8) Spray dry the concentrated solution, the inlet air temperature of spray drying is 180-200℃, the outlet air temperature is 80-90℃, and the feeding speed is 10-15mL / min, and the dried tremella extract is obtained.

9. A method of preparing the cosmetic composition having a moisturizing and soothing effect according to any one of claims 1 to 8, characterized in that, The method comprises the following steps: S101: The lotus water and the butanediol are added to a reaction kettle, stirred uniformly to obtain a mixed solution A; S102: The yeast polysaccharide, the tremella extract and the beta-glucan are sequentially added to the mixed solution A, stirred at 30-40 DEG C until completely dissolved to obtain a mixed solution B; S103: The 1,2-hexanediol and the p-hydroxyacetophenone are added to the mixed solution B, continue to stir for 15-25 minutes to obtain the cosmetic composition with the moisturizing and soothing effect.

10. The method for preparing the cosmetic composition having a moisturizing and soothing effect according to claim 9, wherein the step of mixing the water-soluble polymer and the water-soluble humectant is performed at a temperature of 20 to 40°C. The stirring speed in S101 is 100-200 r / min; the stirring speed in S102 is 300-500 r / min; and the stirring speed in S103 is 200-300 r / min.

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