Chinese herbal medicine preparation for enhancing growth of pseudosciaena crocea as well as preparation process and feeding method of Chinese herbal medicine preparation

By combining compound extracts of traditional Chinese medicine, fermented feed of compound traditional Chinese medicine, and chitosan oligosaccharides, the problems of slow growth and susceptibility to disease in juvenile to adult large yellow croaker have been solved, achieving rapid growth, healthy development, and efficient utilization of nutrients, thus improving the growth performance and immunity of large yellow croaker.

CN121154725APending Publication Date: 2025-12-19ZHEJIANG ZHOUSHAN FISHERIES RES INST (ZHOUSHAN AQUATIC TECH PROMOTION STATION ZHOUSHAN AQUATIC ANIMAL EPIDEMIC PREVENTION & QUARANTINE CENT)
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Patent Information

Application Number
CN202511282787.X
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-09-09
Publication Date
2025-12-19

AI Technical Summary

Technical Problem

In the current large yellow croaker farming process, the growth cycle from juvenile to adult fish is long, their immunity is low, and they are susceptible to diseases. Traditional feed lacks targeted functional components, and long-term use of antibiotics leads to increased drug resistance. The fish are also susceptible to oxidative stress. Existing Chinese herbal medicine formulas have limited functions and fail to meet the needs of rapid growth and healthy development.

Method used

The preparation process involves a combination of traditional Chinese medicine compound extracts, compound traditional Chinese medicine fermented feed, and chitosan oligosaccharides. The preparation process includes the preparation of traditional Chinese medicine compound extracts, the preparation of compound traditional Chinese medicine fermented feed, and the preparation of traditional Chinese medicine compound preparations. The feeding method is to feed twice a day for 25-30 consecutive days, with a 15-day interval between feedings. The feeding amount is 3-4% of the fish's body weight.

Benefits of technology

It significantly improves the growth rate of juvenile to adult fish, increases daily weight gain and final weight, shortens the breeding cycle, enhances immunity, improves fish meat quality, reduces nutrient waste, avoids short-term interruption of effects, and ensures stable growth-promoting and immunity-enhancing effects throughout the entire growth cycle.

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Abstract

The invention relates to the technical field of Chinese herbal medicine preparation, in particular to a Chinese herbal medicine preparation for enhancing growth of pseudosciaena crocea as well as a preparation process and a feeding method. The Chinese herbal medicine preparation for enhancing the growth of the large yellow croaker comprises the following components in parts by weight: 8-10 parts of Chinese herbal medicine compound extract, 2-3 parts of compound Chinese herbal medicine fermented feed and 0.1-0.3 part of chitosan oligosaccharide. The preparation is designed for the larimichthys crocea from the juvenile fish stage to the adult fish stage, contains astragalus membranaceus, honeysuckle, radix isatidis, schisandra chinensis and the like, is matched with fermented feed and chitosan oligosaccharide, and the three components cooperate to promote growth, increase daily weight gain and shorten the period. The feed is fed twice a day in a mode of continuous 25-30 days and 15 days at intervals, and is matched with feeding habits, so that the continuous action of active ingredients is ensured, the long-term metabolic burden is reduced, and the effect is stably exerted.
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Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of Chinese herbal medicine preparation, in particular to a Chinese herbal medicine preparation for enhancing the growth of large yellow croaker, a preparation process and a feeding method. BACKGROUND

[0002] Large yellow croaker is an important marine economic fish in China. Due to its tender and nutritious meat, strong market demand, and continuous expansion of the breeding scale, it has become one of the main varieties in marine aquaculture industry. With the increasing degree of intensive aquaculture, the growth stage of large yellow croaker from juvenile to adult has become the key to improving the efficiency of aquaculture. The fish body growth demand is strong during this stage, and it has high requirements for nutrition supply, immune function and environmental adaptability. Its growth rate and health status directly determine the final yield and quality of aquaculture.

[0003] However, there are still many technical bottlenecks in the current large yellow croaker breeding process: first, the growth cycle from juvenile to adult is relatively long, and traditional feed mainly relies on basic nutrient ratio, lacking targeted functional ingredients, which is difficult to efficiently promote protein synthesis and metabolism of fish body, resulting in low daily weight gain rate and prolonged breeding cycle; second, pathogenic bacteria and viruses are prone to breed in the breeding water and fish gut, and the immunity of juvenile to adult is not fully mature, which is easy to be infected and cause diseases. Traditional prevention and control methods mainly rely on antibiotics or chemical bacteriostatic agents, which not only easily lead to the increase of drug resistance of pathogenic bacteria, but also cause drug residues, endangering food safety and breeding ecology; third, fish body is easily affected by oxidative stress during growth, and accumulation of free radicals will damage cell structure and destroy metabolic enzyme activity stability, further inhibiting growth performance.

[0004] As a natural feed additive, Chinese herbal medicine has the advantages of wide source, safety and no residue, and multiple functions such as promoting growth, antibacterial and antioxidant, which has great application potential in aquaculture. However, there are few special Chinese herbal medicine formulae for large yellow croaker from juvenile to adult stage at present, and the existing formulae are simple in composition and single in function, which cannot realize the synergistic effect of promoting growth, strengthening immunity and improving digestion. At the same time, the scientific feeding method for the feeding habit and metabolic law of fish body at this stage is also not perfect, which leads to the inability of the additive to continuously and stably play its role, and it is difficult to meet the needs of intensive aquaculture for the rapid growth and healthy development of large yellow croaker.

[0005] Therefore, a Chinese herbal medicine preparation for enhancing the growth of large yellow croaker, a preparation process and a feeding method are developed to solve the above problems. SUMMARY

[0006] In view of the deficiencies of the prior art, the purpose of the present application is to provide a Chinese herbal medicine preparation for enhancing the growth of large yellow croaker, a preparation process and a feeding method.

[0007] The application provides a Chinese herbal medicine preparation for enhancing the growth of large yellow croaker, which comprises the following components in parts by weight:

[0008] 8-10 parts by weight of the Chinese herbal medicine compound extract, 2-3 parts by weight of the compound Chinese herbal medicine fermented feed and 0.1-0.3 parts by weight of chitooligosaccharide.

[0009] The Chinese herbal medicine compound extract comprises 15-20 parts by weight of honeysuckle, 10-15 parts by weight of isatis root, 12-18 parts by weight of astragalus root and 5-10 parts by weight of schisandra chinensis.

[0010] The application further provides a preparation process of the Chinese herbal medicine preparation for enhancing the growth of large yellow croaker, which comprises the following steps:

[0011] S1: preparation of the Chinese herbal medicine compound extract;

[0012] The 15-20 parts by weight of honeysuckle, 10-15 parts by weight of isatis root, 12-18 parts by weight of astragalus root and 5-10 parts by weight of schisandra chinensis are ground and crushed, then subjected to ethanol extraction, flash extraction, filtration, centrifugation, suction filtration and concentration by a rotary evaporator to obtain the Chinese herbal medicine compound extract;

[0013] S2: preparation of the compound Chinese herbal medicine fermented feed;

[0014] The residue in S1 is fermented to obtain the compound Chinese herbal medicine fermented feed;

[0015] S3: preparation of the Chinese herbal medicine compound preparation;

[0016] The 8-10 parts by weight of the Chinese herbal medicine compound extract, 2-3 parts by weight of the compound Chinese herbal medicine fermented feed and 0.1-0.3 parts by weight of chitooligosaccharide are mixed to prepare the Chinese herbal medicine compound preparation.

[0017] As a preferred aspect, S1: preparation of the Chinese herbal medicine compound extract, specifically comprises the following steps:

[0018] S1.1: the 15-20 parts by weight of honeysuckle, 10-15 parts by weight of isatis root, 12-18 parts by weight of astragalus root and 5-10 parts by weight of schisandra chinensis are dried, mixed and crushed, and then sieved through an 80-100 mesh sieve to obtain the mixed Chinese herbal medicine powder;

[0019] S1.2: 10-12 parts by weight of mixed Chinese herbal medicine powder is added into 50-60 parts by weight of 70wt% ethanol aqueous solution, then enzyme is inactivated in a boiling water bath for 10-12 min, ultrasonic extraction is carried out at 60-70℃ for 30-40 min, then flash extraction is carried out, to obtain an extract, the extract is centrifuged at 1000-1200 rpm for 10-12 min to obtain Chinese herbal medicine residues and supernatant, the supernatant obtained by centrifugation is subjected to suction filtration, then concentrated by a rotary evaporator to obtain a Chinese herbal medicine composite extract.

[0020] As a preferred aspect, S2: preparation of compound Chinese herbal medicine fermented feed, specifically comprising the following steps:

[0021] S2.1: after high-pressure steam sterilization, the soybean meal powder, honey sugar, corn powder, rice bran and bran are prepared into a fermentation medium according to a ratio of 4-8:1-2:1-2:1:1;

[0022] S2.2: after high-pressure sterilization of the Chinese herbal medicine residues, the Chinese herbal medicine residues and the fermentation medium are mixed according to a ratio of 8-10:2, then 50% of the total amount of deionized water is added, then mixed bacteria are inoculated and mixed uniformly, then placed into a fermentation bag and sealed with a sealing machine, then incubated at 37℃ for 48-50h to obtain compound Chinese herbal medicine fermented feed.

[0023] As a preferred aspect, S3: preparation of Chinese herbal medicine composite preparation, specifically comprising the following steps:

[0024] 8-10 parts by weight of Chinese herbal medicine composite extract, 2-3 parts by weight of compound Chinese herbal medicine fermented feed and 0.1-0.3 parts by weight of chitosan oligosaccharide are mixed and stirred at 200-300 rpm for 20-30 min to obtain a Chinese herbal medicine composite preparation.

[0025] As a preferred aspect, the mixed bacteria in step S2.2 are composed of Enterococcus faecalis, Saccharomyces cerevisiae and Bacillus subtilis, and are mixed according to a ratio of 1:1:1.

[0026] As a preferred aspect, the inoculation amount in step S2.2 is 2-4wt%.

[0027] The application also provides a feeding method of the Chinese herbal medicine preparation for enhancing the growth of large yellow croaker, comprising:

[0028] The Chinese herbal medicine is uniformly mixed into the basic feed at a weight ratio of 1.5-2.5%, and is fed to the juvenile fish stage (body weight≥50g) to the adult fish stage (≤800g), the feeding frequency is twice a day (08:00, 16:00), the continuous feeding time is 25-30 days, the interval is 15 days, the feeding amount is 3-4% of the fish body weight per day.

[0029] The application has the following advantages:

[0030] 1. The preparation of this invention contains astragalus polysaccharides that can promote protein synthesis and metabolism in large yellow croaker and accelerate cell proliferation. Extracts from honeysuckle and isatis root contain chlorogenic acid, indirubin, and other components, exhibiting broad-spectrum antibacterial and antiviral effects, inhibiting pathogenic bacteria and viruses in the intestines and aquaculture water of large yellow croaker. Schisandra chinensis contains schisandrin, which has antioxidant properties, clearing free radicals in the large yellow croaker, reducing oxidative stress damage to cells, and maintaining stable metabolic enzyme activity. The fermented feed, after fermentation with probiotics such as Enterococcus faecalis and Saccharomyces cerevisiae, produces small molecule peptides, amino acids, and digestive enzymes that significantly improve the digestibility and absorption of nutrients in the feed, reducing nutrient waste. Chitosan oligosaccharides can promote intestinal absorption of minerals and aid in bone and muscle development. The synergistic effect of these three components effectively increases the growth rate from juvenile to adult stages, improves daily weight gain and final weight, and shortens the aquaculture cycle.

[0031] 2. The formulation of this invention is designed for the critical growth stages of large yellow croaker from juvenile stage (≥50g) to adult stage (≤800g). During this stage, large yellow croaker has vigorous growth requirements and high demands for nutrition and immunity. The feeding frequency of twice daily matches the feeding habits of large yellow croaker. The cycle of continuous feeding for 25-30 days plus a 15-day interval can not only continuously provide effective components and avoid short-term interruption of effects, but also reduce the metabolic burden of long-term use, ensuring stable growth-promoting and immune-strengthening effects throughout the entire growth cycle. Attached Figure Description

[0032] Figure 1 This is a flowchart illustrating the preparation process of the herbal preparation for enhancing the growth of large yellow croaker used in an embodiment of the present invention. Detailed Implementation

[0033] To enable those skilled in the art to better understand the technical solutions of this invention, the technical solutions of this invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of this invention.

[0034] Example 1: Preparation process and feeding method of a traditional Chinese medicine preparation for enhancing the growth of large yellow croaker, referencing... Figure 1 ,include:

[0035] S1: Preparation of compound extracts from traditional Chinese medicine

[0036] S1.1: Dry 15 parts by weight of honeysuckle, 10 parts by weight of isatis root, 12 parts by weight of astragalus and 5 parts by weight of schisandra, mix them and pulverize them, and pass them through an 80-mesh sieve to obtain mixed Chinese herbal medicine powder.

[0037] S1.2: 10 parts by weight of mixed Chinese herbal medicine powder was added to 50 parts by weight of 70wt% ethanol aqueous solution, then enzyme was inactivated in a boiling water bath for 10 min, ultrasonic extraction was carried out at 60℃ for 30 min, then flash extraction was carried out, to obtain an extract, the extract was centrifuged at 1000 rpm for 10 min to obtain Chinese medicine residue and supernatant, the supernatant obtained by centrifugation was suction filtered, then concentrated by a rotary evaporator to obtain a Chinese herbal medicine composite extract;

[0038] S2: Preparation of compound Chinese herbal medicine fermented feed

[0039] S2.1: The soybean meal powder, honey sugar, corn powder, rice bran and bran after high-pressure steam sterilization were prepared into a fermentation medium according to a ratio of 4:1:1:1:1;

[0040] S2.2: After high-pressure sterilization of the Chinese medicine residue, the Chinese medicine residue and the fermentation medium were mixed according to a ratio of 8:2, then deionized water was added in a total amount of 50%, then mixed bacteria were inoculated, the inoculation amount was 2wt%, mixed uniformly, put into a fermentation bag and sealed with a sealing machine, then constant temperature fermentation was carried out at 37℃ for 48h to obtain a compound Chinese herbal medicine fermented feed;

[0041] The mixed bacteria were composed of Enterococcus faecalis, Saccharomyces cerevisiae and Bacillus subtilis, and were mixed according to a ratio of 1:1:1;

[0042] S3: Preparation of Chinese herbal medicine composite preparation

[0043] 8 parts by weight of Chinese herbal medicine composite extract, 2 parts by weight of compound Chinese herbal medicine fermented feed and 0.1 part by weight of chitosan oligosaccharide were mixed and stirred at 200 rpm for 20 min to obtain a Chinese herbal medicine composite preparation;

[0044] Feeding method:

[0045] The Chinese herbal medicine composite preparation was uniformly mixed into the basic feed at a weight ratio of 1.5%, and was fed to the juvenile fish period (body weight≥50g) to the adult fish period (≤800g), the feeding frequency was twice a day (08:00, 16:00), the feeding was continuously carried out for 25 days, the period was repeated every 15 days, and the feeding amount was 3% of the fish body weight per day.

[0046] Example 2, a preparation process of a Chinese herbal medicine preparation for enhancing the growth of large yellow croaker, feeding method, reference Figure 1 , comprising:

[0047] S1: Preparation of Chinese herbal medicine composite extract

[0048] S1.1: 15 parts by weight of honeysuckle, 10 parts by weight of isatis root, 12 parts by weight of astragalus and 5 parts by weight of schisandra were dried and mixed, crushed, and passed through a 100 mesh sieve to obtain mixed Chinese herbal medicine powder;

[0049] S1.2: 10 parts by weight of mixed Chinese herbal medicine powder was added to 50 parts by weight of 70wt% ethanol aqueous solution, then enzyme was inactivated in boiling water bath for 12 min, 70℃ ultrasonic extraction was carried out for 40 min, then flash extraction was carried out, to obtain an extract, the extract was centrifuged at 1200 rpm for 12 min to obtain Chinese medicine residue and supernatant, the supernatant obtained by centrifugation was suction filtered, then concentrated by a rotary evaporator to obtain a Chinese herbal medicine composite extract;

[0050] S2: Preparation of compound Chinese herbal medicine fermented feed

[0051] S2.1: The soybean meal powder after high-pressure steam sterilization, honey sugar, corn powder, rice bran, bran were prepared into a fermentation medium according to a ratio of 4:1:1:1:1;

[0052] S2.2: After high-pressure sterilization of Chinese medicine residue, mixed according to a ratio of Chinese medicine residue: fermentation medium of 8:2, then inoculated with mixed bacteria, the inoculation amount was 2wt%, mixed uniformly, put into a fermentation bag and sealed with a sealing machine, then constant temperature fermentation at 37℃ for 50h to obtain compound Chinese herbal medicine fermented feed;

[0053] The mixed bacteria were composed of Enterococcus faecalis, Saccharomyces cerevisiae and Bacillus subtilis, and were mixed according to a ratio of 1:1:1;

[0054] S3: Preparation of Chinese herbal medicine composite preparation

[0055] 8 parts by weight of Chinese herbal medicine composite extract, 2 parts by weight of compound Chinese herbal medicine fermented feed and 0.1 part by weight of chitosan oligosaccharide were mixed and stirred at 300 rpm for 30 min to obtain a Chinese herbal medicine composite preparation;

[0056] Feeding method:

[0057] The Chinese herbal medicine composite preparation was uniformly mixed into the basic feed at a weight ratio of 1.5%, and was fed to the juvenile fish period (body weight≥50g) to the adult fish period (≤800g), the feeding frequency was twice a day (08:00, 16:00), and the feeding amount was 3% of the fish body weight per day.

[0058] Example 3, a preparation process of a Chinese herbal medicine preparation for enhancing the growth of large yellow croaker, feeding method, for reference Figure 1 , comprising:

[0059] S1: Preparation of Chinese herbal medicine composite extract

[0060] S1.1: 20 parts by weight of honeysuckle, 15 parts by weight of isatis root, 18 parts by weight of astragalus, and 10 parts by weight of schisandra were dried and mixed and crushed, and then passed through an 80-mesh sieve to obtain mixed Chinese herbal medicine powder;

[0061] S1.2: 12 parts by weight of mixed Chinese herbal medicine powder were added to 60 parts by weight of 70wt% ethanol aqueous solution, then enzyme was inactivated in a boiling water bath for 10 min, ultrasonic extraction was performed at 60℃ for 30 min, then flash extraction was performed, to obtain an extract, the extract was centrifuged at 1000 rpm for 10 min to obtain Chinese herbal medicine residues and supernatant, the supernatant obtained by centrifugation was suction filtered, then concentrated by a rotary evaporator to obtain a Chinese herbal medicine composite extract;

[0062] S2: Preparation of compound Chinese herbal medicine fermented feed

[0063] S2.1: After high-pressure steam sterilization, soybean meal powder, honey sugar, corn powder, rice bran, and bran were prepared into a fermentation medium in a ratio of 8:2:2:1:1;

[0064] S2.2: After high-pressure sterilization of the Chinese herbal medicine residues, the Chinese herbal medicine residues and the fermentation medium were mixed in a ratio of 10:2, then deionized water was added in a total amount of 50%, then mixed bacteria were inoculated, the inoculation amount was 4wt%, mixed uniformly, sealed with a sealing machine, then incubated at 37℃ for 48h to obtain a compound Chinese herbal medicine fermented feed;

[0065] The mixed bacteria were composed of Enterococcus faecalis, Saccharomyces cerevisiae, and Bacillus subtilis, and were mixed in a ratio of 1:1:1;

[0066] S3: Preparation of Chinese herbal medicine composite preparation

[0067] 10 parts by weight of Chinese herbal medicine composite extract, 3 parts by weight of compound Chinese herbal medicine fermented feed, and 0.3 parts by weight of chitosan oligosaccharide were mixed and stirred at 200 rpm for 20 min to obtain a Chinese herbal medicine composite preparation;

[0068] Feeding method:

[0069] The Chinese herbal medicine composite preparation was uniformly mixed into the basic feed at a weight ratio of 2.5%, and was fed to the juvenile fish stage (body weight≥50g) to the adult fish stage (≤800g), the feeding frequency was twice a day (08:00, 16:00), and the feeding amount was 4% of the fish body weight per day.

[0070] Comparative Example 1, compared with Example 1, the difference between Comparative Example 1 and Example 1 is that Comparative Example 1 removes chitosan oligosaccharide in step S3, and the remaining steps remain unchanged, denoted as Comparative Example 1.

[0071] Comparative Example 2, compared with Example 1, the difference between Comparative Example 2 and Example 1 is that Comparative Example 2 removes Chinese herbal medicine composite extract and compound Chinese herbal medicine fermented feed in step S3, and the remaining steps remain unchanged, denoted as Comparative Example 2.

[0072] A control group was set up, only fed with basic feed, and the rest remained unchanged.

[0073] The weight gain rate and final weight of the large yellow croaker fed with examples 1-3, comparative examples 1-2 and the control group were determined, and the determination results are shown in Table 1.

[0074] Table 1. Determination results of growth performance of large yellow croaker

[0075]

[0076] As can be seen from the data in Table 1, the addition of Chinese herbal medicine and chitosan oligosaccharide can effectively improve the growth rate of juvenile fish to adult fish stage, increase the weight gain rate and final weight, improve the feed utilization efficiency, and shorten the breeding cycle.

[0077] The muscle nutritional components of the large yellow croaker fed with examples 1-3, comparative examples 1-2 and the control group were determined, and the determination results are shown in Table 2.

[0078] Table 2. Determination of muscle nutritional components

[0079] Crude protein / % Crude fat / % Moisture / % Ash / % Example 1 18.31 7.35 73.42 1.26 Example 2 18.29 7.32 73.45 1.25 Example 3 18.32 7.34 74.43 1.25 Comparative Example 1 17.58 7.49 73.65 1.28 Comparative Example 2 17.97 7.71 73.24 1.08 Control (basal feed) 17.4 7.85 73.53 1.22

[0080] As can be seen from the data in Table 2, the addition of Chinese herbal medicine and chitosan oligosaccharide can synergistically optimize the comprehensive quality of fish meat.

[0081] The muscle taste index of the large yellow croaker fed with examples 1-3, comparative examples 1-2 and the control group was determined, and the determination results are shown in Table 3.

[0082] Table 3. Determination of muscle taste index

[0083] Hardness / N Springiness / mm Cohesiveness / N Chewiness / mJ Example 1 12.43 0.84 3.12 2.73 Example 2 12.32 0.83 3.09 2.78 Example 3 12.39 0.84 3.11 2.73 Comparative Example 1 10.07 0.82 2.76 2.28 Comparative Example 2 7.75 0.78 1.99 1.53 Control (basal feed) 3.98 0.79 1.29 1.26

[0084] As can be seen from the data in Table 3, the addition of Chinese herbal medicine and chitosan oligosaccharide has a significant advantage in improving the muscle quality of large yellow croaker.

[0085] It should be understood that those skilled in the art can make improvements or changes according to the above description, and all these improvements and changes shall fall within the protection scope of the appended claims of the present application. The parts not described in detail in the specification belong to the prior art known to those skilled in the art.

Claims

1. A Chinese herbal medicine preparation for enhancing the growth of Pseudosciaena crocea, characterized in that, Comprise: 8-10 parts by weight of Chinese herbal medicine composite extract, 2-3 parts by weight of compound Chinese herbal medicine fermented feed and 0.1-0.3 parts by weight of chitooligosaccharide; the Chinese herbal medicine composite extract comprises 15-20 parts by weight of honeysuckle, 10-15 parts by weight of isatis root, 12-18 parts by weight of astragalus root and 5-10 parts by weight of schisandra chinensis.

2. The preparation process of the Chinese herbal medicine preparation for enhancing the growth of Pseudosciaena crocea according to claim 1, characterized in that, Comprise: S1: preparation of Chinese herbal medicine composite extract; 15-20 parts by weight of honeysuckle, 10-15 parts by weight of isatis root, 12-18 parts by weight of astragalus root and 5-10 parts by weight of schisandra chinensis are ground and crushed, then extracted with ethanol, then flash extracted, filtered to obtain dregs and supernatant, the supernatant obtained by centrifugation is filtered, then concentrated by rotary evaporator to obtain Chinese herbal medicine composite extract; S2: preparation of compound Chinese herbal medicine fermented feed; The dregs in S1 are fermented to obtain compound Chinese herbal medicine fermented feed; S3: preparation of Chinese herbal medicine composite preparation; 8-10 parts by weight of Chinese herbal medicine composite extract, 2-3 parts by weight of compound Chinese herbal medicine fermented feed and 0.1-0.3 parts by weight of chitooligosaccharide are mixed to prepare Chinese herbal medicine composite preparation.

3. The preparation process of the Chinese herbal medicine preparation for enhancing the growth of Pseudosciaena crocea according to claim 2, characterized in that, S1: preparation of Chinese herbal medicine composite extract, specifically comprising the following steps: S1.1: 15-20 parts by weight of honeysuckle, 10-15 parts by weight of isatis root, 12-18 parts by weight of astragalus root and 5-10 parts by weight of schisandra chinensis are dried and mixed, then crushed and passed through an 80-100 mesh sieve to obtain mixed Chinese herbal medicine powder; S1.2: 10-12 parts by weight of mixed Chinese herbal medicine powder are added to 50-60 parts by weight of 70wt% ethanol aqueous solution, then enzyme is inactivated in boiling water bath for 10-12 min, ultrasonic extraction is carried out at 60-70℃ for 30-40 min, then flash extraction is carried out, the extract is centrifuged at 1000-1200 rpm for 10-12 min to obtain Chinese medicine dregs and supernatant, the supernatant obtained by centrifugation is filtered, then concentrated by rotary evaporator to obtain Chinese herbal medicine composite extract.

4. The preparation process of the Chinese herbal medicine preparation for enhancing the growth of Pseudosciaena crocea according to claim 3, characterized in that, S2: preparation of compound Chinese herbal medicine fermented feed, specifically comprising the following steps: S2.1: soybean meal powder, honey sugar, corn powder, rice bran and bran after high pressure steam sterilization are mixed to prepare fermentation medium according to the ratio of 4-8:1-2:1-2:1:1; S2.2: the Chinese medicine dregs are high pressure sterilized, then mixed according to the ratio of Chinese medicine dregs: fermentation medium 8-10:2, then added with 50% of total amount of deionized water, then inoculated with mixed bacteria, mixed uniformly, put into a fermentation bag and sealed with a sealing machine, then constant temperature fermentation is carried out at 37℃ for 48-50h to obtain compound Chinese herbal medicine fermented feed.

5. The preparation process of the Chinese herbal medicine preparation for enhancing the growth of Pseudosciaena crocea according to claim 4, characterized in that, S3: preparation of Chinese herbal medicine composite preparation, specifically comprising the following steps: 8-10 parts by weight of Chinese herbal medicine composite extract, 2-3 parts by weight of compound Chinese herbal medicine fermented feed and 0.1-0.3 parts by weight of chitooligosaccharide are mixed and stirred at 200-300 rpm for 20-30 min to obtain Chinese herbal medicine composite preparation.

6. The preparation process of the Chinese herbal medicine preparation for enhancing the growth of Pseudosciaena crocea according to claim 5, characterized in that, The mixed bacteria in step S2.2 are composed of enterococcus faecalis, saccharomyces cerevisiae and bacillus subtilis, and are mixed according to the ratio of 1:1:

1.

7. The process for preparing the Chinese herbal preparation for enhancing the growth of Pseudosciaena crocea according to claim 6, characterized in that, The inoculation amount in step S2.2 is 2-4wt%.

8. The method of claim 1, wherein the Chinese herbal medicine is fed to the Pseudosciaena crocea in an amount of 0.1-0.5% of the weight of the Pseudosciaena crocea. The Chinese herbal medicine is mixed into the basic feed at 1.5-2.5% by weight, and is fed to the young fish (body weight≥50g) to adult fish (≤800g), the feeding frequency is twice a day (08:00, 16:00), and the feeding is continuously carried out for 25-30 days, the cycle is repeated every 15 days, and the feeding amount is 3-4% of the fish body weight per day.