External traditional Chinese medicine composition for soothing liver-qi stagnation as well as preparation method and application of external traditional Chinese medicine composition

Abstract

The invention belongs to the technical field of traditional Chinese medicine external preparations, and provides an external traditional Chinese medicine composition for soothing liver-qi stagnation and a preparation method and application thereof. The external traditional Chinese medicine composition for soothing liver-qi stagnation comprises the following components in parts by weight: 3-6 parts of radix bupleuri, 3-6 parts of fingered citron, 3-6 parts of rose, 3-6 parts of pericarpium citri reticulatae, 3-6 parts of mint, 3-6 parts of rhizoma acori graminei, 1-3 parts of cinnamon, 1-3 parts of sandalwood and 3-6 parts of raw rhizoma atractylodis macrocephalae. The traditional Chinese medicine composition has the effects of soothing the liver, regulating qi, warming, dredging, inducing resuscitation, invigorating the spleen and replenishing qi, has the effects of soothing liver-qi stagnation and tranquilizing mind by nourishing the heart, and is used for treating depression, anorexia and hypochondriac distension caused by unsmooth mood. The external traditional Chinese medicine composition for soothing liver-qi stagnation has a remarkable curative effect on depression on the basis of syndrome differentiation and treatment of traditional Chinese medicine, and has a great social value for widening the current depression treatment method.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of external use of traditional Chinese medicine, and relates to an external use traditional Chinese medicine composition for soothing the liver and relieving depression as well as a preparation method and application thereof. BACKGROUND

[0002] Depression is a common chronic and severe mental illness, which is mainly manifested by continuous and long-term emotional depression, sleep disorders, anhedonia and mental debilitation, and seriously affects people's daily work and life. Depression has a complex etiology and is not a simple functional mental disorder, but a disease involving genetic, psychological, biochemical and social environmental factors, based on which hypotheses are formed to explain the pathogenesis of depression from different angles. Representative theories include hypothalamic-pituitary-adrenal axis dysfunction hypothesis, monoamine neurotransmitter and its receptor hypothesis, neural plasticity and neurotrophic factor hypothesis, cellular and molecular mechanism hypothesis, inflammation and cytokine hypothesis, excitatory amino acid hypothesis, and intestinal flora imbalance hypothesis.

[0003] At present, drug treatment is still the main treatment method for depression, among which synthetic second-generation antidepressants are the first choice for clinical treatment of depression, but they have the disadvantages of large adverse reactions, single target, long onset time and easy drug resistance.

[0004] Traditional Chinese medicine treatment has the advantages of multiple targets, multiple pathways, strong synergistic effect, overall regulation, high safety and the like, which is exactly in line with the nature of depression, a disease with multiple causes.

[0005] At present, products on the market for emotional stagnation and depression mainly fall into the following categories: Oral traditional Chinese medicine / health care products: such as classic oral traditional Chinese medicine or health care products like Xiaoyao Pill and Modified Xiaoyao Pill, which can regulate liver qi by oral administration; Chemical sedative and tranquilizing drugs: such as antipsychotic drugs, which are mainly used for the treatment of anxiety and insomnia, but have dependence and side effects, and are not suitable for daily emotional regulation; Essential oil-based aromatherapy products (the closest prior art): The core components of Chinese patent CN202011252216.9 (a sleep-inducing pharmaceutical composition, health care product and preparation method and application thereof) are silkworm excrement, chrysanthemum, mulberry leaf, cassia seed, chuanxiong rhizome, angelica root, baizhi and wind-protecting herb. The target of this technology is "hyperactivity of liver yang", and the treatment method is mainly "clearing and purging" and "suppressing". The target of "heart-opening and depression-relieving oil" is "stagnation of liver qi", and the treatment method is "soothing and dredging". For depression, chest distention, sighing, poor appetite and other typical liver depression symptoms caused by emotional stagnation and qi stagnation, the prior art mainly based on clearing and purging may aggravate the stagnation of qi due to its cold and cool properties.

[0006] The core components of Chinese patent CN202211286726.7 (a traditional Chinese medicine composition for relieving depression and tranquilizing the mind, and a pharmaceutical preparation and application thereof) are lavender, Platycladus orientalis, Schizonepeta, Acorus gramineus and Angelica sinensis. The scheme clearly proposes the goal of "relieving depression and tranquilizing the mind". The composition reflects the idea of combining traditional Chinese medicine with Western medicine. The idea can be summarized as the combination of "aromatic dredging of qi" and "nourishing the heart and tranquilizing the mind". However, the structure of the composition is not clear.

[0007] The prior art mainly has the following deficiencies: The composition lacks support from traditional Chinese medicine theory: Most of the existing products or like Example 1 have single pathogenesis positioning, or like Example 2 do not follow the compatibility principle of "monarch, minister, assistant and guide" in traditional Chinese medicine, and fail to form a synergistic system of soothing the liver, regulating qi, invigorating the spleen, opening the orifices and tranquilizing the mind. "Open Heart and Relieve Depression Oil" is precisely positioned for "liver qi stagnation", guided by classical traditional Chinese medicine theory, with Chaihu and Foshou as monarchs for soothing the liver, with rose and dried tangerine peel as ministers for regulating qi, with menthol and Acorus gramineus as assistants for opening the orifices, and with cassia, sandalwood and atractylodes as guides for warming and dredging the spleen and protecting the root. The pure traditional Chinese medicine external compound has a clear structure of monarch, minister, assistant and guide, and simultaneously soothes the liver, regulates qi, opens the orifices and invigorates the spleen. The precise compatibility ensures concentrated drug effect, liver-spleen simultaneous treatment and synergistic effect. The product plays a role through both nasal olfactory nerve stimulation therapy and transdermal absorption therapy.

[0008] Neglecting transdermal absorption and meridian theory: The existing products do not fully consider the transdermal absorption of drug active ingredients through specific acupoints (such as Xingxiang, Dantian, Shengzhe, Qimen and Neiguan) to directly reach the disease and dredge the meridians, thereby limiting their therapeutic effect.

[0009] In view of this, the present inventors have carried out in-depth research on this demand, and thus the present case has been produced. SUMMARY

[0010] In view of this, the present application provides a traditional Chinese medicine composition for soothing the liver and relieving depression, as well as a preparation method and application thereof. The present application has a significant effect on depression through nasal olfactory nerve stimulation therapy and transdermal absorption therapy for soothing the liver and relieving depression and regulating emotions on the basis of traditional Chinese medicine syndrome differentiation and treatment, which has great social value for broadening the current methods for treating depression.

[0011] The present application also provides a preparation method of the above-mentioned traditional Chinese medicine composition for soothing the liver and relieving depression.

[0012] The present application also provides the application of the above-mentioned traditional Chinese medicine composition for soothing the liver and relieving depression in the preparation of a medicine for treating soothing the liver and relieving depression and nourishing the heart and tranquilizing the mind.

[0013] The technical scheme adopted by the present application to achieve the above-mentioned purposes is as follows: The first aspect of the present application provides a liver-soothing and depression-resolving external use traditional Chinese medicine composition, which contains the following components in parts by weight: Radix Bupleuri 3-6 parts, Fructus Citri Sarcodactylis 3-6 parts, Rosa rugosa 3-6 parts, Pericarpium Citri Reticulatae 3-6 parts, Herba Menthae 3-6 parts, Rhizoma Acori Graminei 3-6 parts, Cinnamomum cassia 1-3 parts, Santalum album 1-3 parts, and Rhizoma Atractylodis Macrocephalae 3-6 parts.

[0014] As a preferred technical solution of the present application, the composition contains the following components in parts by weight: Radix Bupleuri 4 parts, Fructus Citri Sarcodactylis 4 parts, Rosa rugosa 4 parts, Pericarpium Citri Reticulatae 4 parts, Herba Menthae 4 parts, Rhizoma Acori Graminei 4 parts, Cinnamomum cassia 2 parts, Santalum album 2 parts, and Rhizoma Atractylodis Macrocephalae 2 parts.

[0015] As a preferred technical solution of the present application, the composition contains the following components in parts by weight: Radix Bupleuri 3 parts, Fructus Citri Sarcodactylis 6 parts, Rosa rugosa 6 parts, Pericarpium Citri Reticulatae 3 parts, Herba Menthae 4 parts, Rhizoma Acori Graminei 3 parts, Cinnamomum cassia 1 part, Santalum album 3 parts, and Rhizoma Atractylodis Macrocephalae 3 parts.

[0016] As a preferred technical solution of the present application, the composition contains the following components in parts by weight: Radix Bupleuri 6 parts, Fructus Citri Sarcodactylis 3 parts, Rosa rugosa 4 parts, Pericarpium Citri Reticulatae 6 parts, Herba Menthae 3 parts, Rhizoma Acori Graminei 6 parts, Cinnamomum cassia 3 parts, Santalum album 1 part, and Rhizoma Atractylodis Macrocephalae 1 part.

[0017] The second aspect of the present application provides a preparation method of the above-mentioned liver-soothing and depression-resolving external use traditional Chinese medicine composition, which comprises the following steps: taking the medicinal materials in the weight ratio, mixing the medicinal materials with vegetable oil in a proportion, extracting through a vegetable oil water bath constant temperature, filtering, and obtaining the oil agent of the external use traditional Chinese medicine composition of the present application.

[0018] As a preferred technical solution of the present application, the mass ratio of the traditional Chinese medicine composition to the vegetable oil is 1:3-3.5.

[0019] As a preferred technical solution of the present application, the vegetable oil is sesame oil, sunflower seed oil, camellia seed oil, corn oil or grape seed oil.

[0020] The third aspect of the present application provides the use of the liver-soothing and depression-resolving external use traditional Chinese medicine composition in the preparation of a medicine for preventing or treating liver-soothing and depression-resolving, heart-nourishing and spirit-peace.

[0021] The present application also provides the use of the liver-soothing and depression-resolving external use traditional Chinese medicine composition in the preparation of an antidepressant medicine.

[0022] TCM believes that the location of depression is in the brain, and is closely related to heart, liver, spleen, kidney and other organs, the main pathogenesis is qi stagnation, emotional discomfort, and the treatment should be to regulate qi and relieve depression, regulate qi, and ease the general treatment principle. The characteristics of traditional Chinese medicine have unique advantages and potential in the prevention and treatment of depression with complex pathogenesis, but there are still many problems to be further explored in the prevention and treatment of depression by traditional Chinese medicine.

[0023] The external use traditional Chinese medicine composition for soothing liver and relieving depression of the present application is composed of Chaihu, Foshou, Rose flower, Chenpi, Bohe, Shichangpu, Rugu, Tanshang, and Shengbaishu. Among them, Chaihu is the main medicine for soothing liver and relieving depression, and regulating liver qi. Foshou soothes liver and regulates qi, and harmonizes stomach to stop pain. Rose flower moves qi and relieves depression, and promotes blood circulation to stop pain. Chenpi regulates qi, invigorates spleen, dries dampness and reduces phlegm. Bohe assists Chaihu in soothing liver, and clears and benefits the head. Shichangpu dries dampness and opens the stomach, and awakens the mind and improves intelligence. Rugu supplements fire and assists yang, dispels cold and stops pain, warms the channels and vessels, and moves qi and warms the middle. Baishu invigorates the spleen and benefits qi, dries dampness and reduces water, and is the main medicine for supplementing qi and invigorating the spleen. The whole prescription has the effects of soothing liver and regulating qi, warming and unblocking the orifices, and invigorating the spleen and benefiting qi, and has the functions of soothing liver and relieving depression, and nourishing the heart and tranquilizing the mind. It is used for the depression caused by emotional discomfort, and the depression caused by emotional discomfort.

[0024] The present application is based on the theory of TCM "liver governing dispersion", "aromatic opening orifices" and "meridians and acupoints". Its working process is a "smell-touch-meridian" trinity synergistic process: ①Smell pathway (smell): when the product is opened and used instantly, volatile aromatic components such as Bohe, Rose flower, Tanshang and Foshou are inhaled through the nasal cavity, stimulating the olfactory nerve, rapidly producing a pleasant, relaxed feeling, and immediately relieving depression.

[0025] ②Transdermal absorption and local effect (touch): the oil is applied to the skin, especially the weak stratum corneum of the above-mentioned acupoints. The components such as Bohe and Rugu in the prescription are natural transdermal enhancers, which can carry the liver-soothing and qi-regulating components such as Chaihu and Chenpi to penetrate the skin barrier.

[0026] ③Meridian and acupoint conduction (meridian): after being applied to specific acupoints (such as Dantian and Qiduan), the properties (pungent, fragrant, warm and dispersing) of the medicine are conducted through the meridian system, directly reaching the liver meridian and pericardium meridian, and playing a role in smoothing qi, relieving depression and relieving chest.

[0027] The key point of the present application is: to create a compound compatibility of "soothing liver- regulating qi-unblocking orifices-strengthening the spleen" The technical problem to be solved: to solve the problem that the existing aromatic therapy products have single function and are lack in systemically regulating liver depression.

[0028] Specific scheme: take Chaihu and Foshou as the monarchs for soothing liver; take Meiguihua and Chenpi as the ministers for regulating qi; take Bojutang and Shichangpu as the adjuvants for opening the orifices; take Rougui, Tanxiang and Baizhu as the assistants for warming and dredging the spleen. The whole prescription takes into account multiple aspects of emotional problems (depression, qi stagnation, mental confusion, and spleen deficiency), and under the guidance of TCM theory, a more comprehensive conditioning is formed.

[0029] The second key point of the application: clear targeted use scheme combined with TCM meridian theory The technical problem solved by the application: improve the precision of external use products, and directly send the drug efficacy to the disease site through the meridian system.

[0030] Specific scheme: in the specification, it is clearly instructed that the user applies the product to key acupoints such as the center of the chest, the period gate and the inner pass. Combined with the meridian theory under the TCM system, the synergistic effect of "1+1>2" is achieved.

[0031] The beneficial effects of the external use traditional Chinese medicine composition for soothing liver and relieving depression are: The cumulative immobility time in the tail suspension test can reflect the depression and despair state of experimental animals. The immobility time of mice in the tail suspension test is calculated to evaluate the despair of mice. The external use traditional Chinese medicine composition for soothing liver and relieving depression can significantly improve the forced tail suspension and forced swimming of depressed mice, effectively improve depression, and has a significant effect in preventing and / or treating depression.

[0032] Rapid onset, multi-dimensional effect: instant relief of emotions through olfaction, and long-term conditioning of somatic symptoms (hypochondriac distension and anorexia) of liver depression through transdermal absorption and meridian conduction.

[0033] High safety, convenient to use: avoids gastrointestinal discomfort or liver and kidney metabolic burden that may be caused by oral drugs, and provides a new choice for users who are unwilling or unsuitable to take oral drugs. The external use method is simple and easy to use.

[0034] Strict prescription, treating both symptoms and root causes: targeting emotional symptoms of "depression", regulating qi and spleen and stomach function of "stagnation" and "stagnation", and fundamentally improving the overall state of liver qi stagnation. BRIEF DESCRIPTION OF DRAWINGS

[0035] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the following will briefly introduce the drawings needed to be used in the embodiments or prior art description. Obviously, the drawings in the following description are only some embodiments of the present application, and other drawings can be obtained by those skilled in the art without creative labor.

[0036] Figure 1 Comparison of cumulative immobility time of mice in each group in the tail suspension test, different lowercase letters on the top of the columnar chart represent significant differences (P<0.05) between different groups.

[0037] Figure 2 Cumulative immobility time of each group of mice in forced swimming test, different lowercase letters on the top of the column chart represent significant differences between different groups (P<0.05). DETAILED DESCRIPTION

[0038] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative work fall within the scope of protection of the present application.

[0039] The various chemical reagents and components used in the embodiments of the present application are obtained through conventional commercial channels unless otherwise specified. The products of different manufacturers do not have a significant effect on the results.

[0040] Example 1: A liver-soothing and depression-relieving external use traditional Chinese medicine composition Radix Bupleuri 4 parts, Citrus medica 4 parts, Rosa rugosa 4 parts, Pericarpium Citri Reticulatae 4 parts, Mentha haplocalyx 4 parts, Acorus gramineus 4 parts, Cinnamomum cassia 2 parts, Santalum album 2 parts, and Rhizoma Atractylodis 2 parts.

[0041] The preparation method comprises the following steps:

[0042] Example 2: A liver-soothing and depression-relieving external use traditional Chinese medicine composition Radix Bupleuri 3 parts, Citrus medica 6 parts, Rosa rugosa 6 parts, Pericarpium Citri Reticulatae 3 parts, Mentha haplocalyx 4 parts, Acorus gramineus 3 parts, Cinnamomum cassia 1 part, Santalum album 3 parts, and Rhizoma Atractylodis 3 parts.

[0043] The preparation method comprises the following steps: The preparation method comprises the following steps:

[0044] Example 3: A liver-soothing and depression-relieving external use traditional Chinese medicine composition Radix Bupleuri 6 parts, Citrus medica 3 parts, Rosa rugosa 4 parts, Pericarpium Citri Reticulatae 6 parts, Mentha haplocalyx 3 parts, Acorus gramineus 6 parts, Cinnamomum cassia 3 parts, Santalum album 1 part, and Rhizoma Atractylodis 1 part.

[0045] The preparation method comprises the following steps: The weight proportioned medicinal materials are mixed with plant oil at a mass ratio of 1:3.5, constant temperature extraction is carried out through sesame oil water bath, the water bath temperature is 95 DEG C, the water bath time is 80 min, filtration is carried out, and the oil preparation of the external use traditional Chinese medicine composition is obtained.

[0046] Example 4: An external use traditional Chinese medicine composition for soothing liver and relieving depression 4 parts of Chaihu, 6 parts of Foshou, 3 parts of rose flower, 3 parts of Chenpi, 3 parts of Bohe, 4 parts of Shichangpu, 2 parts of Rougui, 2 parts of Tanxiang, and 3 parts of Shengbaishu.

[0047] The preparation method comprises the following steps: The weight proportioned medicinal materials are mixed with plant oil at a mass ratio of 1:3.5, constant temperature extraction is carried out through sesame oil water bath, the water bath temperature is 95 DEG C, the water bath time is 80 min, filtration is carried out, and the oil preparation of the external use traditional Chinese medicine composition is obtained.

[0048] Example 5: An external use traditional Chinese medicine composition for soothing liver and relieving depression 6 parts of Chaihu, 4 parts of Foshou, 3 parts of rose flower, 4 parts of Chenpi, 6 parts of Bohe, 3 parts of Shichangpu, 3 parts of Rougui, 3 parts of Tanxiang, and 2 parts of Shengbaishu.

[0049] The preparation method comprises the following steps: The weight proportioned medicinal materials are mixed with plant oil at a mass ratio of 1:3, constant temperature extraction is carried out through sesame oil water bath, the water bath temperature is 95 DEG C, the water bath time is 90 min, filtration is carried out, and the oil preparation of the external use traditional Chinese medicine composition is obtained.

[0050] Example 6: An external use traditional Chinese medicine composition for soothing liver and relieving depression 3 parts of Chaihu, 6 parts of Foshou, 6 parts of rose flower, 3 parts of Chenpi, 4 parts of Bohe, 4 parts of Shichangpu, 1 part of Rougui, 3 parts of Tanxiang, and 3 parts of Shengbaishu.

[0051] The preparation method comprises the following steps: The weight proportioned medicinal materials are mixed with plant oil at a mass ratio of 1:3, constant temperature extraction is carried out through sesame oil water bath, the water bath temperature is 95 DEG C, the water bath time is 90 min, filtration is carried out, and the oil preparation of the external use traditional Chinese medicine composition is obtained.

[0052] Comparative Example 1 4 parts of Chaihu, 4 parts of rose flower, 4 parts of Chenpi, 4 parts of Bohe, 4 parts of Shichangpu, 2 parts of Rougui, 2 parts of Tanxiang, and 2 parts of Shengbaishu.

[0053] Compared with Example 1, the difference lies in that Foshou is not contained, and the other components and the amount are unchanged.

[0054] The rest is the same as Example 1.

[0055] Comparative Example 2 Radix Bupleuri 4 parts, Citrus medica 4 parts, Rosa rugosa 4 parts, Pericarpium Citri Reticulatae 4 parts, Mentha haplocalyx 4 parts, Cinnamomum cassia 2 parts, Santalum album 2 parts, Atractylodes macrocephala koidz 2 parts.

[0056] Comparative Example 2 differs from Example 1 in that it does not contain Acorus gramineus, and the other components and amounts are the same.

[0057] The rest is the same as Example 1.

[0058] Comparative Example 3 Radix Bupleuri 4 parts, Citrus medica 4 parts, Rosa rugosa 4 parts, Pericarpium Citri Reticulatae 4 parts, Mentha haplocalyx 4 parts, Acorus gramineus 4 parts, Cinnamomum cassia 2 parts, Santalum album 2 parts, Atractylodes macrocephala koidz 2 parts.

[0059] Comparative Example 3 differs from Example 1 in that the preparation method is different.

[0060] The preparation method comprises the following steps: Take the weight ratio of Radix Bupleuri, Citrus medica, Pericarpium Citri Reticulatae, Acorus gramineus, Cinnamomum cassia, Santalum album, and Atractylodes macrocephala koidz, and add 10 times the amount of water to the total weight of the medicinal materials, decoct and extract 2 times, each decocting and extracting for 1.5 h, and 10 minutes before the end of the second decocting, add the weight ratio of Rosa rugosa and Mentha haplocalyx, combine the decoction, filter, and concentrate to a clear extract with a relative density of 1.10, to obtain the traditional Chinese medicine composition.

[0061] Comparative Example 4 Radix Bupleuri 4 parts, Citrus medica 4 parts, Rosa rugosa 4 parts, Pericarpium Citri Reticulatae 4 parts, Mentha haplocalyx 4 parts, Acorus gramineus 4 parts, Cinnamomum cassia 2 parts, Santalum album 2 parts, Atractylodes macrocephala koidz 2 parts.

[0062] Comparative Example 4 differs from Example 1 in that the preparation method is different.

[0063] The preparation method comprises the following steps: (1) Take the weight ratio of Radix Bupleuri, Citrus medica, Pericarpium Citri Reticulatae, Acorus gramineus, and Cinnamomum cassia, and extract the volatile oil with water to obtain a mixed volatile oil and dregs, and add 12 times the amount of water to the total weight of the medicinal materials, and distill for 2.5 h; (2) Add water to the dregs obtained in step (1) and the weight ratio of Atractylodes macrocephala koidz, and decoct and extract 2 times, each time adding 6 times the weight of water, and each time decocting and extracting for 0.5 h, and 10 minutes before the end of the second decocting, add the weight ratio of Rosa rugosa and Mentha haplocalyx, combine the decoction, filter, and concentrate to a clear extract with a relative density of 1.10, to obtain clear extract 1; (3) Take the mixed volatile oil obtained in step (1) and the clear extract 1 obtained in step (2) and mix them evenly to obtain the traditional Chinese medicine composition.

[0064] Animal Experiment 1. Experimental method ICR mice 90, body weight 18-22g, conventional feed feeding, free drinking water. After 1 week of acclimation, according to the body weight, randomly divided into blank control group, model control group, experiment 1 group, experiment 2 group, experiment 3 group, control 1 group, control 2 group, control 3 group, control 4 group, 10 in each group.

[0065] The corresponding products of each group are applied to the corresponding acupoints. The selected acupoints include: Xingxiang point ", "Shanzhong acupoint", "Shenque acupoint", "Qimen acupoint", "Neiguan acupoint" and "Taiyang acupoint". Experiment 1 group uses 12.5g / kg (calculated as crude drug) of the external use traditional Chinese medicine composition of example 1, experiment 2 group uses 12.5g / kg (calculated as crude drug) of the external use traditional Chinese medicine composition of example 2, experiment 3 group uses 12.5g / kg (calculated as crude drug) of the external use traditional Chinese medicine composition of example 3, control 1 group uses 12.5g / kg (calculated as crude drug) of the external use traditional Chinese medicine composition of comparative example 1, control 2 group uses 12.5g / kg (calculated as crude drug) of the external use traditional Chinese medicine composition of comparative example 2, control 3 group uses 12.5g / kg (calculated as crude drug) of the external use traditional Chinese medicine composition of comparative example 3, control 4 group uses 12.5g / kg (calculated as crude drug) of the external use traditional Chinese medicine composition of comparative example 4.

[0066] The blank control group and the model control group of mice smear the same amount of sesame oil. Each group of mice is given medicine once a day, and the continuous administration is 2 weeks.

[0067] On the 14th day, the model control group and each administration group were injected intraperitoneally with 0.1mL / 10g of 2.5mg / kg reserpine solution prepared with normal saline, and the blank control group was injected intraperitoneally with the same volume of normal saline.

[0068] 2. Tail suspension test The tail end of the mouse is pasted 1cm from the tail tip on the top of the box. The head of the mouse is downward, about 20cm from the bottom of the box. The experiment lasts for 5min. When the suspended mouse does not struggle obviously, it is considered to be static. The cumulative immobility time of the mouse within 4min after the experiment is recorded.

[0069] Forced swimming test The experiment is carried out in a organic glass cylinder (25cmx10cmx15cm), and the water temperature is controlled at 22-26℃ when swimming. The water level is controlled so that the mouse cannot touch the bottom of the cylinder when swimming. Forced swimming for 6min, and the cumulative immobility time of the mouse within 4min after observation and recording.

[0070] 3. Statistical method The data were processed by GraphPad Prism 7.0 software, and the measurement data were expressed as ±s, and the comparison between groups was analyzed by one-way ANOVA, and P<0.05 was considered to be statistically significant.

[0071] 4. Experimental results 4.1 Comparison of forced tail suspension and forced swimming results of mice in each group Table 1 Comparison of forced tail suspension and forced swimming results of mice in each group Note: The same column data with different lowercase letters represent significant differences (P<0.05) between different groups.

[0072] Compared with the blank control group, the cumulative immobility time of the model control group mice in the tail suspension test was significantly increased.

[0073] Compared with the model control group, the cumulative immobility time of mice in the tail suspension test in the experimental group 1, the experimental group 2, the experimental group 3, and the control group was significantly reduced. Compared with the control group 1-4, the cumulative immobility time of the experimental group 1, the experimental group 2, and the experimental group 3 was significantly reduced. The results are shown in Table 1, Figure 1 .

[0074] Compared with the blank control group, the cumulative immobility time of the model control group mice in the forced swimming test was significantly increased.

[0075] Compared with the model control group, the cumulative immobility time of mice in the forced swimming test in the experimental group 1, the experimental group 2, the experimental group 3, and the control group was significantly reduced. Compared with the control group 1-4, the cumulative immobility time of the experimental group 1, the experimental group 2, and the experimental group 3 was significantly reduced. The results are shown in Table 1, Figure 2 .

[0076] The cumulative immobility time in the tail suspension test can reflect the depression and despair state of experimental animals, and the tail suspension immobility time of mice is calculated to evaluate the despair of mice. The external use of traditional Chinese medicine composition for soothing liver and relieving depression can significantly improve the forced tail suspension and forced swimming of depressed mice, and effectively improve depression.

[0077] From the above examples, the present application provides an external use of traditional Chinese medicine composition for soothing liver and relieving depression, and its preparation method and application. The comparative example 1 does not contain bergamot, the comparative example 2 does not contain Acorus gramineus, the comparative example 3 and the comparative example 4 change the preparation method, and the anti-depression effect of the comparative examples 1-4 is weaker than that of the traditional Chinese medicine composition of the present application. The components of the traditional Chinese medicine composition of the present application synergize with each other, and act together, and the traditional Chinese medicine is extracted during the preparation process, which can fully utilize various effective components of traditional Chinese medicine, improve the effect of traditional Chinese medicine composition, and the prepared traditional Chinese medicine composition has a significant prevention and treatment effect on depression.

[0078] The preferred embodiments of the present application have been described. It is to be understood that the application is not limited to the above specific embodiments, and that devices and structures not described in detail should be understood to be implemented in the ordinary way in the art; any person skilled in the art can make many possible changes and modifications to the technical solutions of the present application, or modify them into equivalent embodiments with equivalent changes, without departing from the scope of the technical solutions of the present application, which does not affect the essential content of the present application.

[0079] Therefore, any simple modification, equivalent change and modification made to the above embodiments according to the technical essence of the present application, without departing from the content of the technical solutions of the present application, still belongs to the scope of protection of the technical solutions of the present application.

Claims

1. A topical traditional Chinese medicine composition for soothing the liver and relieving depression, characterized in that, The aforementioned external Chinese medicine composition for soothing the liver and relieving depression contains the following components by weight: Bupleurum chinense 3-6 parts, Citrus medica var. sarcodactylis 3-6 parts, Rosa rugosa 3-6 parts, Citrus reticulata 3-6 parts, Mentha haplocalyx 3-6 parts, Acorus tatarinowii 3-6 parts, Cinnamomum cassia 1-3 parts, Santalum album 1-3 parts, and Atractylodes macrocephala 3-6 parts.

2. The external application traditional Chinese medicine composition for soothing the liver and relieving depression according to claim 1, characterized in that, The external Chinese medicine composition for soothing the liver and relieving depression is made from the following components by weight: Bupleurum chinense 4 parts, Citrus medica 4 parts, Rosa rugosa 4 parts, Citrus reticulata 4 parts, Mentha haplocalyx 4 parts, Acorus tatarinowii 4 parts, Cinnamomum cassia 2 parts, Santalum album 2 parts, and Atractylodes macrocephala 2 parts.

3. The external application traditional Chinese medicine composition for soothing the liver and relieving depression according to claim 1, characterized in that, The external Chinese medicine composition for soothing the liver and relieving depression is made from the following raw materials, by weight: Bupleurum chinense 3 parts, Citrus medica 6 parts, Rosa rugosa 6 parts, Citrus reticulata 3 parts, Mentha haplocalyx 4 parts, Acorus tatarinowii 3 parts, Cinnamomum cassia 1 part, Santalum album 3 parts, and Atractylodes macrocephala 3 parts.

4. The external application traditional Chinese medicine composition for soothing the liver and relieving depression according to claim 1, characterized in that, The external Chinese medicine composition for soothing the liver and relieving depression is made from the following raw materials, by weight: Bupleurum chinense 6 parts, Citrus medica 3 parts, Rosa rugosa 4 parts, Citrus reticulata 6 parts, Mentha haplocalyx 3 parts, Acorus tatarinowii 6 parts, Cinnamomum cassia 3 parts, Sandalwood 1 part, and Atractylodes macrocephala 1 part.

5. The method for preparing the external application traditional Chinese medicine composition for soothing the liver and relieving depression according to any one of claims 1-4, characterized in that, The preparation method is as follows: take the medicinal materials in the specified weight ratio, mix the medicinal materials with vegetable oil in a certain proportion, extract the vegetable oil by constant temperature water bath, filter, and obtain the external Chinese medicine composition oil of the present invention.

6. The method for preparing the external application traditional Chinese medicine composition for soothing the liver and relieving depression according to claim 5, characterized in that, The water bath temperature is 85-95℃, and the water bath time is 80-100 minutes.

7. The method for preparing the external application traditional Chinese medicine composition for soothing the liver and relieving depression according to claim 5, characterized in that, The mass ratio of the traditional Chinese medicine composition to the plant oil is 1:3-3.

5.

8. The method for preparing the external application traditional Chinese medicine composition for soothing the liver and relieving depression according to claim 5, characterized in that, The vegetable oil is sesame oil, olive oil, sunflower seed oil, camellia seed oil, corn oil, or grape seed oil.

9. The use of the external Chinese medicine composition for soothing the liver and relieving depression according to any one of claims 1-4 in the preparation of drugs for preventing or treating soothing the liver and relieving depression, nourishing the heart and calming the mind.

10. The use of the topical Chinese medicine composition for soothing the liver and relieving depression according to any one of claims 1-4 in the preparation of antidepressant drugs for prevention or treatment.

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