A weight loss composition containing ginger extract and a preparation method and application thereof

Abstract

The present application provides a kind of ginger extract-containing weight loss composition and its preparation method and application, it is related to health care product technical field.The weight loss composition includes the following raw materials: ginger extract, cauliflower extract, acanthopanax extract, elderberry extract, phosphorus shrimp oil, red bean powder and vitamin.The present application significantly improves the weight loss and lipid-lowering effect of the composition by the interaction of specific raw materials ginger extract, cauliflower extract, elderberry extract and phosphorus shrimp oil, improves the metabolism of the body by adding specific compound vitamin, suppresses appetite by adding red bean powder, improves satiety, improves the immunity of the body by adding acanthopanax extract, improves exercise tolerance, cooperates with other components, further improves the weight loss and lipid-lowering effect, and has better stability.

Description

Technical Field

[0001] This invention relates to the field of health product technology, specifically to a weight-loss composition containing ginger extract, its preparation method, and its application. Background Technology

[0002] Obesity refers to a significant degree of overweight and excessive fat accumulation, a condition caused by excessive accumulation of body fat, especially triglycerides. It is not simply weight gain, but a state of excessive accumulation of body fat tissue. Excessive food intake or changes in metabolism lead to excessive fat accumulation, resulting in excessive weight gain and causing or inducing pathological and physiological changes in the body.

[0003] The harmful effects of obesity on the human body include: ① Obese individuals have many complications, including a significantly higher incidence of cerebral embolism, heart failure, coronary heart disease, hypertension, diabetes, and gallstones compared to the general population. More seriously, obese individuals have a significantly shorter lifespan. ② Obese people often experience heat intolerance, excessive sweating, fatigue, lower limb edema, varicose veins, and dermatitis in skin folds. Severely obese individuals experience slow movement, difficulty walking, and shortness of breath with slight exertion, affecting their normal lives and potentially leading to loss of labor capacity. ③ Obesity increases adipose tissue, oxygen consumption, and the workload on the heart, causing myocardial hypertrophy, especially increasing the burden on the left ventricle, which can easily induce hypertension over time. Lipid deposition in the arterial walls leads to narrowing and hardening of the lumen, increasing the risk of coronary heart disease, angina pectoris, stroke, and sudden death. ④ The metabolic and endocrine abnormalities associated with obesity often cause various metabolic diseases. ⑤ Obese individuals require more oxygen due to increased weight, but their lungs cannot keep up with this increase in function. Furthermore, abdominal fat accumulation restricts lung movement, leading to hypoxia, difficulty breathing, and ultimately, cardiopulmonary failure. ⑥ Compared to normal-weight individuals, obese individuals have higher cholesterol levels in their bile acids, exceeding the solubility in bile. Therefore, obese individuals are more prone to developing cholesterol gallstones. ⑦ Obesity increases the risks during anesthesia; postoperative wound dehiscence and complications such as aspiration pneumonia are more likely to occur compared to non-obese individuals. ⑧ Increased weight can cause wear and tear or tearing of many joints (such as the spine, shoulders, elbows, hips, and feet), resulting in pain. ⑨ Obesity can lead to various hernias, with hiatal hernia (transposition of the upper stomach into the thoracic cavity) being the most common. Therefore, weight loss is crucial for the health of obese individuals.

[0004] Currently, existing anti-obesity drugs are ineffective due to tolerability and significant chronic toxic side effects, while many traditional herbal extracts hold promise as safe and effective obesity replacement therapies. Ginger, one of the most common spices, contains many phenolic compounds that have shown beneficial properties for human health, including enhancing digestion, reducing inflammation, vomiting, cancer, and metabolic syndrome.

[0005] The study on the weight loss and lipid reduction effects of ginger oil on nutritionally obese rats (Food Science, Yang Zhigang, Zhang Yanping et al., 2007-10-30, Vol. 28, No. 9) disclosed that ginger oil can effectively reduce the weight of obese rats, as well as the weight of peritesticular and perirenal fat, and reduce serum total cholesterol (TC) and serum triglyceride (TG) levels, thus achieving weight loss and lipid reduction.

[0006] Chinese patent CN111034833A discloses a weight-loss and lipid-regulating compound preparation, which, by weight, contains 3-10 parts of bitter orange flower, 3-10 parts of lotus leaf, 3-10 parts of ginger, 1-5 parts of traditional Chinese medicine components, and 1-5 parts of lemon. Chinese patent CN114391653A discloses a sports nutrition tablet for weight loss, comprising the following components: guarana extract, hibiscus powder, green coffee powder, ginger powder, lotus leaf powder, mulberry leaf extract, raspberry powder, orange peel powder, black pepper powder, ginseng powder, epigallocatechin gallate, white kidney bean powder, vitamin B1, vitamin B2, sugar alcohol, microcrystalline cellulose, magnesium stearate, and coating powder. Chinese patent CN103053764A discloses a health tea made from 3-10 parts soluble pearl powder, 5-15 parts lotus leaf extract, 1-5 parts ginger extract, 20-40 parts jujube extract, 5-10 parts dandelion extract, 10-15 parts konjac extract, 1-5 parts L-carnitine, 20-30 parts maltodextrin, and 0.5-2 parts fat-soluble vitamin C. All products covered by the above patents contain ginger.

[0007] In summary, developing a health product with ginger as the main ingredient, optimizing the preparation method of ginger and combining it with other components to achieve better weight loss and lipid reduction effects, and better meet the needs of obese people, is a research direction for researchers in this field. Summary of the Invention

[0008] To address the aforementioned problems, this invention provides a weight-loss composition containing ginger extract, its preparation method, and its application. Under the specific components, proportions, and preparation method of this invention, the prepared composition exhibits better weight-loss and lipid-lowering effects.

[0009] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0010] On one hand, the present invention provides a weight loss composition containing ginger extract, comprising the following ingredients: ginger extract, cauliflower extract, Acanthopanax senticosus extract, elderberry extract, krill oil, red bean powder, and vitamins.

[0011] Preferably, the vitamins are vitamin C, vitamin B6, and vitamin D.

[0012] Preferably, the mass ratio of vitamin C, vitamin B6 and vitamin D is (5-8):(1-3):(2-4).

[0013] More preferably, the mass ratio of vitamin C, vitamin B6 and vitamin D is 6:2:3.

[0014] Preferably, the mass ratio of the ginger extract, cauliflower extract, krill oil and elderberry extract is (20-30):(10-20):(10-15):(5-10).

[0015] More preferably, the mass ratio of the ginger extract, cauliflower extract, krill oil and elderberry extract is 25:15:12:8.

[0016] Preferably, the method for preparing the ginger extract includes the following steps: mixing ginger with an ethanol solution, performing gradient ethanol extraction, combining the extracts, concentrating, and drying.

[0017] More preferably, the preparation method of the ginger extract includes the following steps: pulverizing ginger to 40-60 mesh, adding 30wt%-40wt% ethanol aqueous solution, with the mass ratio of ginger to ethanol aqueous solution being 1g:8-15mL; first extraction at 35-45℃ for 1-1.5h to obtain extract 1 and residue 1; adding 45wt%-55wt% ethanol aqueous solution to residue 1, with the mass ratio of ginger to ethanol aqueous solution being 1g:8-15mL; second extraction at 45-55℃ for 1-1.5h to obtain extract 2 and residue 2; adding 70wt%-80wt% ethanol aqueous solution to residue 2, with the mass ratio of ginger to ethanol aqueous solution being 1g:8-15mL; third extraction at 55-65℃ for 1-1.5h to obtain extract 3; combining extract 1, extract 2 and extract 3, concentrating and drying.

[0018] Preferably, the method for preparing the cauliflower extract includes the following steps: grinding cauliflower and then enzymatically extracting it, concentrating the extract, and drying it.

[0019] More preferably, the preparation method of the cauliflower extract includes the following steps: grinding cauliflower, mixing it with water, adding a compound enzyme, adjusting the pH to 5-6, extracting at 40-50℃ for 1-2 hours, concentrating the extract, and drying.

[0020] Preferably, the complex enzyme is a cellulase and bromelain with an enzyme activity ratio of 40-60U:10-20U.

[0021] More preferably, the complex enzyme is a cellulase and bromelain with an enzyme activity ratio of 50U:15U.

[0022] Preferably, the ratio of the enzymatic activity of the compound enzyme to the mass of cauliflower is 100-150 U / g.

[0023] More preferably, the ratio of the enzymatic hydrolysis activity of the compound enzyme to the mass of cauliflower is 130 U / g.

[0024] Preferably, the ingredients, by weight, include the following: 20-30 parts ginger extract, 10-20 parts cauliflower extract, 3-8 parts Acanthopanax senticosus extract, 5-10 parts elderberry extract, 10-15 parts krill oil, 10-18 parts red bean powder, and 1-4 parts vitamins.

[0025] More preferably, the ingredients, by weight, include the following: 23-28 parts ginger extract, 13-18 parts cauliflower extract, 4-6 parts Acanthopanax senticosus extract, 6-9 parts elderberry extract, 11-14 parts krill oil, 12-16 parts red bean powder, and 2-3 parts vitamins.

[0026] More preferably, by weight, it includes the following ingredients: 25 parts ginger extract, 15 parts cauliflower extract, 5 parts Acanthopanax senticosus extract, 8 parts elderberry extract, 12 parts krill oil, 15 parts red bean powder, and 3 parts vitamins.

[0027] Secondly, the present invention provides a method for preparing the weight loss composition described above, comprising the following steps: mixing the raw materials.

[0028] Thirdly, the present invention provides the application of the weight-loss composition described above in the preparation of health products with weight-loss and lipid-lowering effects.

[0029] Preferably, the health product also includes other excipients acceptable in the health supplement industry.

[0030] Preferably, the health product can be prepared as a solid beverage formulation, a liquid beverage formulation, a nutrition bar, or a powder, etc.

[0031] Compared with the prior art, the present invention has the following beneficial effects:

[0032] 1. This invention significantly enhances the weight loss and lipid-lowering effects through the interaction of specific raw materials: Ginger extract, on the one hand, can activate the AMPK pathway, promote fat oxidation, effectively inhibit weight gain and hyperlipidemia caused by lipid metabolism disorders, and may increase energy consumption through the thermic effect; on the other hand, it can improve glucose tolerance and increase insulin sensitivity. Cauliflower extract can significantly inhibit the activity of pancreatic amylase in the intestine, hindering or delaying the hydrolysis and digestion of the main carbohydrates in food, and reducing the breakdown and absorption of starch and sugars in food. Krill oil is rich in Omega-3 fatty acids, which not only have anti-inflammatory effects but also significantly improve metabolism. Elderberry extract has antioxidant properties, can dissolve fat, and remove turbidity and oil. The interaction of these components significantly enhances the weight loss and lipid-lowering effects of the product.

[0033] 2. The composition of the present invention improves the body's metabolism by adding specific compound vitamins, suppresses appetite and increases satiety by adding red bean powder, enhances the body's immunity and exercise endurance by adding Acanthopanax senticosus extract, and works in conjunction with other components to significantly improve the effects of weight loss and lipid reduction.

[0034] 3. The specific components, combined with specific dosages and preparation methods of this invention, further enhance its effects and improve its stability. Detailed Implementation

[0035] To make the technical means, creative features, achieved objectives, and effects of this invention readily understandable, the invention is further illustrated below with specific embodiments. However, these embodiments are merely preferred embodiments and not all embodiments. Other embodiments obtained by those skilled in the art based on the embodiments described herein without creative effort are all within the scope of protection of this invention. It is worth noting that the raw materials used in this invention are all common commercially available products, and their sources are not specifically limited. The technical and scientific terms used in the embodiments have the meanings commonly understood by those skilled in the art to which this invention pertains.

[0036] The Acanthopanax senticosus extract was purchased from Xi'an Feida Biotechnology Co., Ltd., product number FDBL-162.

[0037] Elderberry extract, manufactured by Xi'an Dingcui Biotechnology Co., Ltd., product number DC-dsg.

[0038] Example 1

[0039] A weight loss composition containing ginger extract

[0040] (1) By weight, it includes the following ingredients: 25 parts ginger extract, 15 parts cauliflower extract, 5 parts Acanthopanax senticosus extract, 8 parts elderberry extract, 12 parts krill oil, 15 parts red bean powder, 1.63 parts vitamin C, 0.55 parts vitamin B6 and 0.82 parts vitamin D.

[0041] The preparation method of ginger extract includes the following steps: ginger is washed, dried, and pulverized to 50 mesh; 35wt% ethanol aqueous solution is added, with the mass ratio of ginger to ethanol aqueous solution being 1g:10mL; the first extraction is performed at 40℃ for 1.5h to obtain extract 1; the residue is added to 50wt% ethanol aqueous solution, with the mass ratio of ginger to ethanol aqueous solution being 1g:12mL; the second extraction is performed at 50℃ for 1.0h to obtain extract 2; the residue is added to 75wt% ethanol aqueous solution, with the mass ratio of ginger to ethanol aqueous solution being 1g:15mL; the third extraction is performed at 60℃ for 1h to obtain extract 3; the extracts are combined, concentrated, and dried.

[0042] The preparation method of cauliflower extract includes the following steps: cauliflower is washed, dried, and ground. After being mixed with water, a compound enzyme (cellulase and bromelain with an enzyme activity ratio of 50U:15U) is added. The enzymatic hydrolysis activity of the compound enzyme and the mass ratio of cauliflower are 130U / g. The pH is adjusted to 5.5, and the extract is extracted at 45℃ for 1.5h. The extract is then concentrated and dried.

[0043] (2) Preparation method of weight loss composition containing ginger extract: Mix the raw materials evenly.

[0044] Example 2

[0045] A weight loss composition containing ginger extract

[0046] (1) The ingredients include the following ingredients by weight: 20 parts ginger extract, 20 parts cauliflower extract, 3 parts Acanthopanax senticosus extract, 10 parts elderberry extract, 10 parts krill oil, 18 parts red bean powder, 0.625 parts vitamin C, 0.125 parts vitamin B6 and 0.25 parts vitamin D.

[0047] The preparation method of ginger extract includes the following steps: ginger is washed, dried, and pulverized to 40 mesh; 30wt% ethanol aqueous solution is added, with the mass ratio of ginger to ethanol aqueous solution being 1g:8mL; the first extraction is performed at 35℃ for 1.5h to obtain extract 1; the residue is added to 55wt% ethanol aqueous solution, with the mass ratio of ginger to ethanol aqueous solution being 1g:15mL; the second extraction is performed at 55℃ for 1.5h to obtain extract 2; the residue is added to 70wt% ethanol aqueous solution, with the mass ratio of ginger to ethanol aqueous solution being 1g:8mL; the third extraction is performed at 55℃ for 1.5h to obtain extract 3; the extracts are combined, concentrated, and dried.

[0048] The preparation method of cauliflower extract includes the following steps: cauliflower is washed, dried, ground, mixed with water, and then a compound enzyme (cellulase and bromelain with an enzyme activity ratio of 40U:10U) is added. The enzymatic hydrolysis activity of the compound enzyme is 100U / g of the cauliflower. The pH is adjusted to 5.0, and the extract is extracted at 40℃ for 2 hours. The extract is then concentrated and dried.

[0049] (2) Preparation method of weight loss composition containing ginger extract: Mix the raw materials evenly.

[0050] Example 3

[0051] A weight loss composition containing ginger extract

[0052] (1) By weight, it includes the following ingredients: 30 parts ginger extract, 10 parts cauliflower extract, 8 parts Acanthopanax senticosus extract, 5 parts elderberry extract, 15 parts krill oil, 10 parts red bean powder, 2.1 parts vitamin C, 0.8 parts vitamin B6 and 1.1 parts vitamin D.

[0053] The preparation method of ginger extract includes the following steps: ginger is washed, dried, and pulverized to 60 mesh; 40wt% ethanol aqueous solution is added, with the mass ratio of ginger to ethanol aqueous solution being 1g:15mL; the first extraction is performed at 45℃ for 1.0h to obtain extract 1; the residue is added to 45wt% ethanol aqueous solution, with the mass ratio of ginger to ethanol aqueous solution being 1g:10mL; the second extraction is performed at 45℃ for 1.5h to obtain extract 2; the residue is added to 80wt% ethanol aqueous solution, with the mass ratio of ginger to ethanol aqueous solution being 1g:15mL; the third extraction is performed at 65℃ for 1.0h to obtain extract 3; the extracts are combined, concentrated, and dried.

[0054] The preparation method of cauliflower extract includes the following steps: cauliflower is washed, dried, ground, mixed with water, and then a compound enzyme (cellulase and bromelain with an enzyme activity ratio of 60U:20U) is added. The enzymatic hydrolysis activity of the compound enzyme and the mass ratio of cauliflower are 150U / g. The pH is adjusted to 5.5, and the extract is extracted at 50℃ for 1 hour. The extract is then concentrated and dried.

[0055] (2) Preparation method of weight loss composition containing ginger extract: Mix the raw materials evenly.

[0056] Comparative Example 1

[0057] A weight-loss composition containing ginger extract, compared with Example 1, differs only in the amount of raw materials used, specifically as follows:

[0058] (1) By weight, it includes the following ingredients: 35 parts ginger extract, 8 parts cauliflower extract, 2 parts Acanthopanax senticosus extract, 3 parts elderberry extract, 8 parts krill oil, 20 parts red bean powder, 3.82 parts vitamin C, 1.27 parts vitamin B6 and 1.91 parts vitamin D.

[0059] The rest is the same as in Example 1.

[0060] Comparative Example 2

[0061] A weight-loss composition containing ginger extract, compared to Example 1, replaces cauliflower extract with white kidney bean powder, krill oil with raspberry powder, and elderberry extract with epigallocatechin gallate. Specifically:

[0062] (1) By weight, it includes the following ingredients: 25 parts ginger extract, 15 parts white kidney bean powder, 5 parts Acanthopanax senticosus extract, 8 parts epigallocatechin gallate, 12 parts raspberry powder, 15 parts red bean powder, 1.63 parts vitamin C, 0.55 parts vitamin B6 and 0.82 parts vitamin D.

[0063] The preparation method of ginger extract includes the following steps: ginger is washed, dried, and pulverized to 50 mesh; 35wt% ethanol aqueous solution is added, with the mass ratio of ginger to ethanol aqueous solution being 1g:10mL; the first extraction is performed at 40℃ for 1.5h to obtain extract 1; the residue is added to 50wt% ethanol aqueous solution, with the mass ratio of ginger to ethanol aqueous solution being 1g:12mL; the second extraction is performed at 50℃ for 1.0h to obtain extract 2; the residue is added to 75wt% ethanol aqueous solution, with the mass ratio of ginger to ethanol aqueous solution being 1g:15mL; the third extraction is performed at 60℃ for 1h to obtain extract 3; the extracts are combined, concentrated, and dried.

[0064] (2) Preparation method of weight loss composition containing ginger extract: Mix the raw materials evenly.

[0065] Comparative Example 3

[0066] A weight loss composition, compared to Example 1, in which ginger extract is replaced with coix seed extract.

[0067] (1) By weight, it includes the following ingredients: 25 parts of coix seed extract, 15 parts of cauliflower extract, 5 parts of Acanthopanax senticosus extract, 8 parts of elderberry extract, 12 parts of krill oil, 15 parts of red bean powder, 1.63 parts of vitamin C, 0.55 parts of vitamin B6 and 0.82 parts of vitamin D.

[0068] The preparation method of cauliflower extract includes the following steps: cauliflower is washed, dried, and ground. After being mixed with water, a compound enzyme (cellulase and bromelain with an enzyme activity ratio of 50U:15U) is added. The enzymatic hydrolysis activity of the compound enzyme and the mass ratio of cauliflower are 130U / g. The pH is adjusted to 5.5, and the extract is extracted at 45℃ for 1.5h. The extract is then concentrated and dried.

[0069] (2) Preparation method of the above weight loss composition: Mix the raw materials evenly.

[0070] Test Example 1

[0071] Stability test

[0072] The stability of the compositions prepared in Examples 1-3 and Comparative Examples 1-2 was determined by HPLC. Gingerol in each composition was tested at 0, 3 and 6 months, and the loss rate of gingerol in the composition was recorded after 3 and 6 months (with the content on the day of preparation as the comparison standard).

[0073] Chromatographic conditions:

[0074] Chromatographic column: C18 reversed-phase column (such as Agilent Grace Smart RP-C18).

[0075] Mobile phase: Acetonitrile-0.1% formic acid aqueous solution (40:60) or acetonitrile-0.1% acetic acid aqueous solution.

[0076] Flow rate: 1.0 mL / min, detection wavelength: 280 nm.

[0077] Table 1. Loss rate of gingerol / %

[0078]

[0079] As shown in Table 1, the weight loss composition prepared by the present invention with specific components and specific dosages has significantly better stability than the weight loss compositions prepared by Comparative Examples 1 and 2.

[0080] Test Example 2

[0081] Animal experiment: Improvement effect of the composition on obese rats

[0082] 1. Materials and methods

[0083] 1.1 Materials

[0084] 1.1.1 Instruments: FA1104N electronic balance (Shanghai Minqiao Precision Scientific Instruments Co., Ltd.), animal balance, TGL-16aR ultra-high-speed centrifuge (Shanghai Anting Scientific Instruments Factory), EPOCH microplate reader (BioTek Corporation, USA), etc.

[0085] 1.1.2 Reagents: High-fat feed (product number: D12451) was purchased from Beijing Huafukang Biotechnology Co., Ltd.;

[0086] Ordinary rat maintenance feed was purchased from Changchun Yisi Experimental Animal Technology Co., Ltd.;

[0087] Hematoxylin-eosin (HE) staining kit was purchased from Beijing Solarbio Science & Technology Co., Ltd.;

[0088] Rat triglyceride (TG) and total cholesterol (TC) detection kits were both purchased from Jiangsu Feiya Biotechnology Co., Ltd.;

[0089] Rat superoxide dismutase (SOD) was purchased from Nanjing Jiancheng Bioengineering Institute, and alanine aminotransferase (ALT) detection kit was from Jiangsu Addison Biotechnology Co., Ltd.

[0090] 1.1.3 Experimental animals: 72 SPF-grade male SD rats, weighing 180 - 220 g, were purchased from Beijing Huafukang Biotechnology Co., Ltd. [License number SCXK(Jing)2024 - 0003]. Natural light cycle lighting (12 h light / dark alternation) was used, the feeding temperature was 22 - 24 °C, and the relative humidity was 55% - 60%.

[0091] 1.2 Model construction and animal grouping

[0092] After one week of adaptive feeding of the rats, they were randomly divided into 9 groups (n = 8). The dosage of ginger powder recommended by the Chinese Dietary Guidelines for Residents and the "body surface area normalization method" for humans and rats were combined to calculate the dosage conversion and the dosage conversion of orlistat (positive control group). The grouping was as follows:

[0093] Model control group, normal control group, positive control group (orlistat, 31.71 mg / kg), experimental example groups (Experimental Example 1 group, Experimental Example 2 group, Experimental Example 3 group, 12.68 mg / kg), and comparative example groups (Comparative Example 1 group, Comparative Example 2 group, Comparative Example 3 group, 12.68 mg / kg).

[0094] Except for the normal control group, which was given a standard maintenance diet, all other groups of rats were given a high-fat diet for 6 weeks to establish an obese rat model. Except for the model control group and the normal control group, which were given 12.68 mg / kg of distilled water by gavage, the positive control group was given orlistat by gavage, and the example group and the comparative group were given their respective prepared compositions by gavage.

[0095] 1.3 Detection Method

[0096] 1.3.1 Observation of rat condition

[0097] Neck circumference, waist circumference, body weight, body length, and food intake were recorded weekly during the feeding period. After the last administration, rats in each group were fasted for 12 hours but allowed free access to water, and their food intake, body weight, and Lee's index were measured and calculated.

[0098] 1.3.2 Detection of physiological indicators (fat and organs) in rats

[0099] After the experiment, the rats were euthanized by cervical dislocation. The liver, kidneys and other organs of the rats were carefully separated, rinsed with 0.9% physiological saline, and excess water was absorbed with filter paper. The mass was weighed and recorded using an electronic balance, and then frozen at -80℃ for subsequent index detection.

[0100] 1.3.3 Detection of serum markers in rats

[0101] After the last administration, rats were fasted for 12 hours without access to water. After anesthesia, blood was collected from the celiac artery. The blood was allowed to stand at room temperature for 30 minutes, then centrifuged at 4000 rpm for 15 minutes at low temperature to separate the serum, which was then stored at -20°C for analysis. Serum levels of TC and TG were measured according to the kit instructions and assay requirements.

[0102] 1.3.4 Detection of liver markers in rats

[0103] Weigh approximately 0.1g of liver tissue sample, grind it thoroughly into a homogenate, and collect the supernatant for testing. Detect alanine aminotransferase (ALT) and superoxide dismutase (SOD).

[0104] 1.4 Data Statistics and Processing:

[0105] Experimental data were recorded using a double-blind method, and the results were analyzed using GraphPad Prism software (Version 10.1.2, USA). Data are expressed as mean ± standard error of the mean. Experimental data between different groups were compared using one-way analysis of variance (ANOVA) and Tukey's test; p < 0.05 was considered statistically significant.

[0106] 1.5 Results

[0107] 1.5.1 Rat Status

[0108] During the experiment, there was no significant difference in food intake among the groups of rats (p>0.05), indicating that the compositions did not affect the rats' food intake and had no effect on their appetite. The condition of the rats in each group was observed. Compared with the normal control group, the body weight of the rats in the model control group was significantly increased at each week. After intervention with the compositions prepared in the examples and comparative examples, the body weight of the rats in each treatment group decreased to varying degrees compared with the model control group. The body weight at week 6 of modeling is shown in Table 2 below. Compared with the normal control group, the body weight of the rats in the model control group was significantly increased, indicating successful modeling. After administration, the body weight of the rats in the example group was significantly lower than that of the rats in the model control group (p<0.01).

[0109] As shown in Table 2, comparisons of rat groups revealed that the Lee's index was significantly higher in the model control group compared to the normal control group (p<0.01). After drug intervention, the Lee's index of the positive control group decreased significantly (p<0.01), and the Lee's index of the example group also decreased significantly (p<0.01). This indicates that the composition prepared in this invention can effectively improve the baseline indicators of high-fat diet-induced obese rats.

[0110] Table 2. Weight at the sixth week of modeling

[0111]

[0112] Note: Compared with the normal control group, && This represents p < 0.01; compared with the model control group, # This means p < 0.5. ## This means p < 0.01.

[0113] 1.5.2 Effects on fat coefficient and organ coefficient in rats

[0114] The fat coefficient refers to the ratio of periepididymal fat mass to body weight in rats, usually expressed as a percentage. This indicator can preliminarily reflect the relative fat content in rats and provide a basis for assessing the degree of obesity. After 6 weeks of high-fat diet feeding, as shown in Table 3 below, the fat coefficient of obese rats in the model control group was significantly increased (p<0.01). After gavage intervention in the example group, the fat coefficient of rats in all groups decreased significantly. Among them, the fat coefficient of rats in the high-dose group was significantly lower than that of the model control group (p<0.05).

[0115] Organ coefficient refers to the ratio of the weight of each organ to the body weight in rats, reflecting fat deposition and metabolic abnormalities in various organs (such as the liver and kidneys) of obese rats. As shown in Table 3, the comparison of organ coefficients among the groups of rats shows that, compared with the normal control group, the liver and kidney coefficients of the model control group rats were significantly increased (p<0.01). After drug intervention, the kidney coefficients of rats in both the positive drug group and the example group were significantly reduced (p<0.05 or p<0.01). Furthermore, the liver coefficient of the example group rats also showed a significant decrease (p<0.05), and the effect was superior to that of the positive drug orlistat. These results suggest that the composition prepared in the examples of this invention may exert a significant weight-loss effect by regulating hepatic lipid deposition or metabolic processes.

[0116] Table 3. Effects on fat coefficient and organ coefficient in rats

[0117]

[0118] Note: Compared with the normal control group, && This represents p < 0.01; compared with the model control group, # This means p < 0.5. ## This means p < 0.01.

[0119] 1.5.3 Effects on lipid levels in rat serum and liver

[0120] Six weeks after the experimental intervention, the serum lipid levels of rats in each group are shown in Table 4. Compared with the normal control group, the serum TC and TG levels of rats in the model control group were significantly increased (p<0.01). The drug intervention in the example group effectively reduced serum TC and TG levels (p<0.01).

[0121] Furthermore, the intervention in the example group showed a better reduction in serum TC levels than the positive control drug orlistat, indicating that the weight-loss composition prepared in this invention can alleviate obesity by inhibiting lipid levels and lipid deposition in serum and liver. Compared to the positive control drug orlistat, the intervention in the example group demonstrated superior effects in reducing serum TC and TG levels. All of the above suggests that the example group may effectively alleviate the obesity process by inhibiting lipid synthesis and accumulation in serum and liver.

[0122] Table 4. Effects on serum lipid levels in rats

[0123]

[0124] Note: Compared with the normal control group, && This represents p < 0.01; compared with the model control group, # This means p < 0.5. ## This means p < 0.01.

[0125] 1.5.4 Effects on oxidative stress levels in rat liver

[0126] As shown in Table 5 below, compared with the normal control group, the levels of SOD and ALT in the liver of rats in the model control group were significantly increased (p<0.01). This indicates that the antioxidant capacity of the liver in obese rats is reduced and the degree of lipid peroxidation is increased. Compared with the model control group, the ALT level in the liver of rats in the positive drug group was significantly reduced (p<0.01), and the SOD level was significantly increased (p<0.01); the ALT level in the liver of rats in the example group was significantly decreased (p<0.01), and the SOD level was significantly increased (p<0.01). This suggests that the composition prepared in the example group of the present invention can effectively improve the antioxidant level of rat liver and alleviate oxidative stress in the liver of obese rats.

[0127] Table 5. Effects on oxidative stress levels in rat liver

[0128]

[0129] Note: Compared with the normal control group, && This represents p < 0.01; compared with the model control group, # This means p < 0.5. ## This means p < 0.01.

[0130] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, and is not intended to limit the scope of protection of the present invention. Simple modifications or equivalent substitutions made by those skilled in the art to the technical solution of the present invention do not depart from the essence and scope of the technical solution of the present invention.

Claims

1. A weight-loss composition containing ginger extract, characterized in that, The ingredients, by weight, include the following: 20-30 parts ginger extract, 10-20 parts cauliflower extract, 3-8 parts Acanthopanax senticosus extract, 5-10 parts elderberry extract, 10-15 parts krill oil, 10-18 parts red bean powder, and 1-4 parts vitamins; wherein the vitamins are a mixture of vitamin C, vitamin B6, and vitamin D in a weight ratio of (5-8):(1-3):(2-4); the preparation method of the ginger extract includes the following steps: mixing ginger with an ethanol solution, gradient ethanol extraction, combining the extracts, concentrating, and drying; The preparation method of the cauliflower extract includes the following steps: grinding cauliflower, mixing with water, adding a compound enzyme, adjusting the pH to 5-6, extracting at 40-50℃ for 1-2 hours, concentrating the extract, and drying; the compound enzyme is cellulase and bromelain with an enzyme activity ratio of 40-60U:10-20U; the enzymatic hydrolysis activity of the compound enzyme and the mass ratio of cauliflower are 100-150U / g.

2. The weight-loss composition according to claim 1, characterized in that, The preparation method of the ginger extract includes the following steps: pulverizing ginger to 40-60 mesh, adding 30wt%-40wt% ethanol aqueous solution, with a ginger mass to ethanol aqueous solution volume ratio of 1g:8-15mL; first extraction at 35-45℃ for 1-1.5h to obtain extract 1 and residue 1; adding 45wt%-55wt% ethanol aqueous solution to residue 1, with a ginger mass to ethanol aqueous solution volume ratio of 1g:8-15mL; second extraction at 45-55℃ for 1-1.5h to obtain extract 2 and residue 2; adding 70wt%-80wt% ethanol aqueous solution to residue 2, with a ginger mass to ethanol aqueous solution volume ratio of 1g:8-15mL; third extraction at 55-65℃ for 1-1.5h to obtain extract 3; combining extract 1, extract 2 and extract 3, concentrating and drying.

3. A method for preparing the weight-loss composition according to any one of claims 1-2, characterized in that, Includes the following steps: Mix the ingredients.

4. The use of the weight-loss composition according to any one of claims 1-2 in the preparation of health products with weight-loss and lipid-lowering effects.

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