Rapid industrial wastewater pollutant detection method based on molecularly imprinted polymer
By selectively adsorbing and regenerating molecularly imprinted polymers, the problems of cumbersome and low-sensitivity industrial wastewater detection methods have been solved, enabling rapid and accurate pollutant detection that is suitable for industrial field applications.
Patent Information
- Application Number
- CN202511715613.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-11-21
- Publication Date
- 2025-12-19
AI Technical Summary
Existing methods for detecting pollutants in industrial wastewater are cumbersome, time-consuming, have low sensitivity, and are easily affected by complex matrices, making it difficult to meet the needs for rapid detection.
Molecularly imprinted polymers were used to pretreat industrial wastewater samples to prepare molecularly imprinted polymers targeting specific pollutants. Through selective adsorption and detection, combined with regeneration treatment, rapid and accurate pollutant detection was achieved.
It improves detection sensitivity and accuracy, simplifies the operation process, reduces costs, is suitable for large-scale applications, and meets the needs of rapid detection of industrial wastewater.
Smart Images
Figure CN121164580A_ABST
Abstract
Description
Technical Field
[0001] This invention belongs to the field of environmental monitoring and analytical chemistry technology, specifically a rapid detection method for industrial wastewater pollutants based on molecularly imprinted polymers. Background Technology
[0002] With rapid industrial development, the discharge of industrial wastewater is increasing daily. Its composition is complex and diverse, containing heavy metal ions, phenolic compounds, aniline compounds, polycyclic aromatic hydrocarbons, pesticide residues, antibiotics, plasticizers, endocrine disruptors, and many other pollutants. These pollutants not only cause serious damage to the ecological environment but also threaten human health and survival. Therefore, effective detection of pollutants in industrial wastewater is crucial.
[0003] Currently, traditional methods for detecting pollutants in industrial wastewater have many drawbacks. For example, some conventional chemical analysis methods are extremely cumbersome, requiring specialized technicians to perform complex sample pretreatment and multi-step detection procedures, consuming significant time and manpower, resulting in low detection efficiency and failing to meet the needs of rapid wastewater detection in industrial production. Furthermore, the complex matrix composition of industrial wastewater easily interferes with the detection process, leading to poor selectivity and low accuracy in the results. For instance, inductively coupled plasma mass spectrometry (ICP-MS) often encounters mass spectral interference due to the presence of common components such as chloride ions, carbon, and sulfur in industrial wastewater when detecting trace metals, causing deviations of up to an order of magnitude in the detection results for metals such as arsenic, chromium, and copper.
[0004] While some existing detection technologies can detect certain pollutants, they face numerous challenges in practical applications. For example, traditional colorimetric and electrochemical analysis methods cannot simultaneously measure multiple ions and have low sensitivity, making them unsuitable for detecting multiple pollutants at low concentrations in industrial wastewater. Furthermore, some advanced detection technologies, such as monitoring systems based on laser-induced breakdown spectroscopy, can simultaneously measure multiple heavy metal elements, but their high equipment costs and complex maintenance hinder their widespread application in industrial settings. Therefore, this invention provides a rapid detection method for industrial wastewater pollutants based on molecularly imprinted polymers. Summary of the Invention
[0005] In order to overcome the shortcomings of the prior art, at least one technical problem raised in the background art is solved.
[0006] The technical solution adopted by this invention to solve its technical problem is: the rapid detection method for industrial wastewater pollutants based on molecularly imprinted polymers, as described in this invention, includes the following steps: S100. Obtain the industrial wastewater sample to be tested, and perform pretreatment on the industrial wastewater sample to remove solid particles or macromolecules that may interfere with subsequent analysis. S200. Prepare a molecularly imprinted polymer targeting a pollutant. The preparation process includes: selecting a template molecule, a functional monomer, a crosslinking agent, and an initiator, mixing them in a preset ratio, forming a polymer matrix through a polymerization reaction, and removing the template molecule after polymerization to obtain a molecularly imprinted polymer with specific binding sites. S300. The pretreated industrial wastewater sample is brought into contact with the molecularly imprinted polymer, so that the target pollutants in the wastewater are selectively adsorbed onto the binding sites of the molecularly imprinted polymer. S400: Separate the molecularly imprinted polymer and the target pollutants it adsorbs, and clean the molecularly imprinted polymer to remove unadsorbed interfering substances. S500. Detect the molecularly imprinted polymer adsorbed with the target pollutant, and quantitatively analyze the content of the target pollutant in the industrial wastewater sample based on the detection results.
[0007] The pretreatment of the industrial wastewater sample in step S100 includes at least one of the following methods: filtration, centrifugation, pH adjustment, solid-phase extraction, or liquid-liquid extraction. Specifically, filtration uses a 0.45-micron pore size filter membrane, centrifugation speed is set to 3000 rpm, pH adjustment range is 4 to 9, solid-phase extraction column uses C18 packing material, and liquid-liquid extraction solvent is selected from methanol, acetonitrile, or dichloromethane.
[0008] The specific process for preparing the molecularly imprinted polymer in step S200 includes: S210. The template molecule, functional monomer, crosslinking agent and initiator are added to the solvent in a molar ratio of 1:4:20:1 to form a prepolymerization mixture; S220. The prepolymer mixture is subjected to a polymerization reaction under the following conditions: temperature 60°C and time 24 hours. Nitrogen gas is used for protection during the reaction to prevent oxidation. S230. The obtained polymer matrix is washed three times with methanol and water in sequence, and dried at 60°C for 12 hours to remove unreacted monomers and impurities. S240. Use an eluent to remove template molecules from the polymer matrix. The eluent is selected from a mixed solution of methanol and acetic acid with a volume ratio of 9:1. The elution time is 6 hours, and finally a molecularly imprinted polymer with the ability to recognize target pollutants is obtained.
[0009] The functional monomer is selected from at least one of acrylamide, methacrylic acid, vinylpyridine, or styrene; the crosslinking agent is selected from at least one of ethylene glycol dimethacrylate, N,N'-methylenebisacrylamide, or trimethylolpropane trimethacrylate; the initiator is selected from at least one of benzoyl peroxide, azobisisobutyronitrile, or potassium persulfate; and the solvent is selected from at least one of methanol, ethanol, acetone, or acetonitrile.
[0010] In step S300, the pretreated industrial wastewater sample is contacted with a molecularly imprinted polymer using at least one of the following methods: oscillatory adsorption, stirred adsorption, column adsorption, or fluid adsorption through a membrane. Specifically, the oscillatory adsorption frequency is 150 times / minute, the stirred adsorption rotation speed is 500 rpm, the column adsorption flow rate is 1 mL / min, and the membrane adsorption pressure is 0.2 MPa.
[0011] In step S400, the molecularly imprinted polymer and its adsorbed target pollutants are separated by centrifugation, filtration, or magnetic separation. Centrifugation is performed at 5000 rpm, filtration uses a 0.22-micron pore size membrane, and magnetic separation uses an external magnetic field with a strength of 0.5 Tesla. The molecularly imprinted polymer is cleaned using pure water, a buffer solution, or a low-concentration eluent, with three washes, each lasting five minutes.
[0012] The detection of the molecularly imprinted polymer adsorbed with the target pollutant in step S500 includes at least one of the following methods: S510. Directly detect physical or chemical signals in molecularly imprinted polymers adsorbed with target pollutants. Detection methods include electrochemical analysis, spectroscopic analysis, chromatography, or mass spectrometry. S520. The adsorbed target pollutant is eluted from the molecularly imprinted polymer, and then the target pollutant in the eluent is detected. The detection methods include high performance liquid chromatography, gas chromatography, gas chromatography-mass spectrometry, liquid chromatography-mass spectrometry, atomic absorption spectrometry, inductively coupled plasma atomic emission spectrometry, or ultraviolet-visible spectrophotometry.
[0013] The target pollutant is selected from at least one of heavy metal ions, phenolic compounds, aniline compounds, polycyclic aromatic hydrocarbons, pesticide residues, antibiotics, plasticizers, or endocrine disruptors.
[0014] The molecularly imprinted polymer exists in the form of microspheres, nanoparticles, films, thin films, or modified electrodes. The diameter of the microspheres ranges from 1 to 10 micrometers, the size of the nanoparticles ranges from 10 to 100 nanometers, the film thickness ranges from 50 to 200 micrometers, the thin film thickness ranges from 1 to 10 micrometers, and the conductive layer thickness of the modified electrode ranges from 0.1 to 1 micrometer.
[0015] The method further includes, after step S500, regenerating the molecularly imprinted polymer to make it reusable. The regeneration process includes eluting the adsorbed target pollutants using an eluent and activating the polymer. The eluent is selected from methanol, ethanol, acetonitrile, or acetone, the elution time is 4 hours, and the activation process uses ultraviolet irradiation at a wavelength of 254 nm for 30 minutes.
[0016] The beneficial effects of this invention are as follows: The rapid detection method for industrial wastewater pollutants based on molecularly imprinted polymers described in this invention effectively eliminates interference from complex matrices in industrial wastewater through the selective adsorption of molecularly imprinted polymers, thereby improving the detection sensitivity and accuracy of target pollutants. Furthermore, the preparation process of molecularly imprinted polymers is simple and low-cost, making them suitable for large-scale production applications. The regeneration treatment of molecularly imprinted polymers allows for multiple reuses, further reducing detection costs. The overall detection process is simple to operate and significantly shortens the detection time, meeting the demand for rapid detection of pollutants in industrial wastewater. Attached Figure Description
[0017] The invention will now be further described with reference to the accompanying drawings.
[0018] Figure 1 This is a schematic flowchart of the rapid detection method for industrial wastewater pollutants based on molecularly imprinted polymers according to the present invention. Detailed Implementation
[0019] To make the technical means, creative features, objectives and effects of this invention easier to understand, the invention will be further described below in conjunction with specific embodiments.
[0020] like Figure 1 As shown in the embodiments of the present invention, the rapid detection method for industrial wastewater pollutants based on molecularly imprinted polymers involves first introducing the industrial wastewater sample into a pretreatment module. In this module, necessary pretreatment operations are performed on the sample to remove solid particles or macromolecules that may interfere with subsequent analysis. Specifically, the pretreatment operations include at least one of filtration, centrifugation, pH adjustment, solid-phase extraction, or liquid-liquid extraction. For example, filtration uses a 0.45-micron pore size filter membrane installed at the inlet of the pretreatment module. Wastewater samples pass through the membrane before entering the subsequent processing area. Centrifugation is performed within the centrifuge chamber of the pretreatment module, set at 3000 rpm, located in the center of the module. pH adjustment is achieved by adding acid or alkali solutions to the wastewater, with a range of 4 to 9. These solutions are stored in the reagent storage unit of the pretreatment module and injected into the wastewater sample via a micro-pump. The solid-phase extraction column uses C18 packing material and is fixed in the extraction area of the module. The wastewater sample passes through the column, resulting in the enrichment of the target components. The liquid-liquid extraction solvent is selected from methanol, acetonitrile, or dichloromethane, stored in the solvent storage unit of the module, and added to the wastewater sample via a valve. All these operations are coordinated and performed by the control system of the pretreatment module to ensure the sample is in a suitable state for subsequent analysis.
[0021] The pretreated wastewater sample then flows into the adsorption module. Prior to this, a molecularly imprinted polymer (MIP) targeting the pollutant needs to be prepared using the MIP preparation module. The MIP preparation module includes a template molecule storage unit, a functional monomer storage unit, a crosslinking agent storage unit, an initiator storage unit, and a polymerization reaction chamber. Template molecules, functional monomers, crosslinking agents, and initiators are taken from their respective storage units and mixed in a molar ratio of 1:4:20:1 to form a prepolymer mixture. This mixture is then transported to the polymerization reaction chamber for polymerization. The reaction conditions are 60°C for 24 hours, and a nitrogen protection device is installed in the reaction chamber to prevent oxidation. After polymerization, the resulting polymer matrix is washed three times sequentially with methanol and water, and then dried at 60°C for 12 hours within the module's drying chamber. Subsequently, an eluent is used to remove the template molecules from the polymer matrix. The eluent is a methanol-acetic acid mixture with a volume ratio of 9:1, and the elution time is 6 hours, ultimately yielding a MIP capable of recognizing the target pollutant. The MIP is then transported to the adsorption module through the module's output port.
[0022] The adsorption module is a key component for achieving selective adsorption of target pollutants. It contains an adsorption chamber filled with a molecularly imprinted polymer prepared by the module. The pretreated wastewater sample contacts the molecularly imprinted polymer through various methods, including oscillation adsorption, stirring adsorption, column adsorption, or fluid-permeable membrane adsorption. Oscillation adsorption is achieved using an oscillator at the bottom of the adsorption chamber at a frequency of 150 times / minute; stirring adsorption is achieved using a stirring paddle within the adsorption chamber at a speed of 500 rpm; column adsorption is achieved using an adsorption column within the adsorption chamber at a flow rate of 1 mL / min; and membrane adsorption is achieved using a membrane module within the adsorption chamber at a pressure of 0.2 MPa. These adsorption methods can be flexibly selected according to actual needs. The design of the adsorption chamber ensures sufficient contact between the wastewater sample and the molecularly imprinted polymer, thereby achieving selective adsorption of the target pollutant.
[0023] After adsorption, the molecularly imprinted polymer containing the target pollutant flows into the separation and cleaning module, where separation and cleaning operations are performed. Separation methods include centrifugation, filtration, or magnetic separation. Centrifugation is performed in the module's centrifuge chamber at a speed of 5000 rpm; filtration is performed using the module's filtration unit with a 0.22-micron pore size membrane; magnetic separation is achieved using the module's magnetic field generator with an applied magnetic field strength of 0.5 Tesla. The separated molecularly imprinted polymer then enters the cleaning unit. The cleaning solution is selected from pure water, buffer solution, or low-concentration eluent. Cleaning is performed three times, with each wash lasting five minutes. The cleaning solution is provided through the module's cleaning solution storage unit. The cleaned molecularly imprinted polymer is then transferred to the detection module.
[0024] The detection module is used to detect molecularly imprinted polymers adsorbed with target pollutants. Detection methods include direct and indirect detection. Direct detection is achieved through the module's detection unit, using methods such as electrochemical analysis, spectroscopic analysis, chromatography, or mass spectrometry. Indirect detection involves first eluting the target pollutant from the molecularly imprinted polymer, then detecting the pollutant in the eluent. Detection methods include high-performance liquid chromatography (HPLC), gas chromatography (GC), gas chromatography-mass spectrometry (GC-MS), liquid chromatography-mass spectrometry (LC-MS), atomic absorption spectrometry (AAS), inductively coupled plasma atomic emission spectrometry (ICP-AES), or ultraviolet-visible spectrophotometry (UV-Vis). The detection results are analyzed by the module's data processing unit to quantitatively determine the content of the target pollutant in the industrial wastewater sample.
[0025] After detection, the molecularly imprinted polymer can be further processed in the regeneration module for reuse. The regeneration process includes eluting the adsorbed target contaminants with an eluent and activating the polymer. The eluent is selected from methanol, ethanol, acetonitrile, or acetone, and the elution time is 4 hours, completed within the module's elution chamber. Activation is achieved using the module's ultraviolet irradiation device at a wavelength of 254 nm for 30 minutes. The regenerated molecularly imprinted polymer can then be returned to the adsorption module for the next round of detection.
[0026] The connections and coordination between the various modules are achieved through pipes, valves, and a control system. For example, the outlet of the pretreatment module is connected to the inlet of the adsorption module via a pipe equipped with a flow meter and valves to control sample flow; the outlet of the adsorption module is connected to the inlet of the separation and cleaning module via another pipe, also equipped with a flow meter and valves; the outlet of the separation and cleaning module is connected to the inlet of the detection module via a pipe, and the outlet of the detection module is connected to the inlet of the regeneration module via a pipe. The operation of the entire system is coordinated by a central control system to ensure that each module completes its operation in a predetermined sequence.
[0027] In practical applications, the method of this invention can be used to detect multiple target pollutants in industrial wastewater, including at least one of heavy metal ions, phenolic compounds, aniline compounds, polycyclic aromatic hydrocarbons, pesticide residues, antibiotics, plasticizers, or endocrine disruptors. The form of the molecularly imprinted polymer can be selected according to specific needs, including microspheres, nanoparticles, membranes, thin films, or modified electrodes. Microspheres have a diameter ranging from 1 to 10 micrometers, nanoparticles have a size ranging from 10 to 100 nanometers, membranes have a thickness of 50 to 200 micrometers, thin films have a thickness of 1 to 10 micrometers, and the conductive layer thickness of the modified electrode is 0.1 to 1 micrometer. Different forms of molecularly imprinted polymers can be adjusted through the module preparation process to meet specific detection requirements.
[0028] The above description covers the complete implementation process of the method of the present invention. The connection and cooperation relationships between the modules are clear and explicit, and the operation steps are detailed and specific, enabling those skilled in the art to successfully implement the technical solution based on the contents of the specification.
[0029] To enable those skilled in the art to fully understand and implement this invention, the specific implementation principle of this invention is further explained below in conjunction with a specific application scenario.
[0030] In practice, the industrial wastewater sample is first introduced into the pretreatment module. Initial solid particle removal is achieved through a filtration unit; a 0.45-micron pore size filter membrane is installed at the module's inlet, and the wastewater sample enters the module after filtration. Next, centrifugation is performed at 3000 rpm to ensure effective separation of suspended particles in the wastewater. Subsequently, the reagent storage unit of the pretreatment module adds acid or alkali solutions to the wastewater via a micro-pump, adjusting the pH to the target range of 4 to 9. During this process, a C18 packing column is used for solid-phase extraction, enriching the target components as the wastewater sample passes through the column. Simultaneously, the solvent storage unit adds liquid-liquid extraction solvents such as methanol or acetonitrile via valve control, further improving the recovery rate of the target pollutants. All of the above operations are coordinated and completed by the pretreatment module's control system, ensuring that the wastewater sample is in a suitable state for subsequent analysis.
[0031] Before the pretreated wastewater sample flows into the adsorption module, the molecularly imprinted polymer preparation module begins operation. Template molecules, functional monomers, crosslinking agents, and initiators are taken from their respective storage units and mixed in a molar ratio of 1:4:20:1 to form a prepolymerization mixture. This mixture is then transported to the polymerization reaction chamber, where it reacts at 60°C for 24 hours under nitrogen protection to prevent oxidation. After polymerization, the resulting polymer matrix is washed three times sequentially with methanol and water, and then dried at 60°C for 12 hours. Subsequently, template molecules are removed using an eluent with a methanol-acetic acid volume ratio of 9:1 for 6 hours, ultimately yielding the molecularly imprinted polymer with recognition capabilities. These molecularly imprinted polymers are then transported to the adsorption module through the module's output port.
[0032] The adsorption module is a key component for achieving selective adsorption of target pollutants. The wastewater sample contacts the molecularly imprinted polymer using oscillatory adsorption, with the oscillator running at a frequency of 150 times per minute to ensure sufficient contact between the wastewater sample and the polymer. The design of the adsorption chamber allows the target pollutant to efficiently bind to the specific sites on the molecularly imprinted polymer, thus achieving selective adsorption. After the adsorption process is complete, the molecularly imprinted polymer containing the target pollutant flows into the separation and cleaning module.
[0033] The separation and cleaning module uses centrifugation to separate the molecularly imprinted polymer from the waste liquid. The centrifuge chamber operates at 5000 rpm to ensure complete separation. The separated polymer then enters the cleaning unit, using pure water as the cleaning solution. The cleaning is performed three times, with each cleaning cycle lasting five minutes. The cleaning solution is supplied by the module's storage unit, ensuring complete removal of any unadsorbed interfering substances. The cleaned polymer is then transferred to the detection module.
[0034] The detection module directly detects molecularly imprinted polymers adsorbed with target pollutants, determining the pollutant content using electrochemical analysis. The detection unit captures the target pollutant via the molecularly imprinted polymer modified on the electrode surface, records changes in the electrochemical signal, and converts the signal into a concentration value for the target pollutant through a data processing unit. This detection method enables highly sensitive and selective quantitative analysis in complex matrices.
[0035] After the initial testing, the molecularly imprinted polymer enters the regeneration module. Methanol is used as the eluent, and the elution time is 4 hours to ensure complete removal of the adsorbed target pollutants. Subsequently, the polymer is activated by irradiation with ultraviolet light at a wavelength of 254 nm for 30 minutes, restoring its initial adsorption performance. The regenerated molecularly imprinted polymer can then be returned to the adsorption module for the next round of testing.
[0036] The connections and coordination between the various modules are achieved through pipelines, valves, and a central control system. For example, the outlet of the pretreatment module is connected to the inlet of the adsorption module via a pipeline, and flow meters and valves on the pipeline ensure precise control of sample flow. The operation of the entire system is uniformly coordinated by the central control system to ensure that each module completes its operation in a predetermined sequence.
[0037] Through the above steps, this invention achieves rapid detection of target pollutants in industrial wastewater. The selective adsorption of molecularly imprinted polymers effectively eliminates interference from complex matrices, significantly improving detection sensitivity and accuracy. Simultaneously, the regeneration process of the molecularly imprinted polymers allows for multiple reuses, reducing detection costs. The overall process is simple to operate, and the detection time is significantly shortened, meeting the demand for rapid detection of pollutants in industrial wastewater.
[0038] All content not described in detail in this specification is prior art known to those skilled in the art, and the model parameters of each electrical appliance are not specifically limited; conventional equipment can be used. Electrical control components not mentioned in this technical solution are prior art and are therefore not shown in the figures, nor will they be described further here.
[0039] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of the invention. Various changes and modifications can be made to the invention without departing from its spirit and scope, and all such changes and modifications fall within the scope of the present invention as claimed. The scope of protection of the present invention is defined by the appended claims and their equivalents.
Claims
1. A rapid detection method for pollutants in industrial wastewater based on molecularly imprinted polymers, characterized in that, Including the following: S100. Provide an industrial wastewater sample to be tested, and pretreat the industrial wastewater sample to remove solid particles or macromolecules that may interfere with subsequent testing. S200. Prepare a molecularly imprinted polymer targeting a pollutant. The preparation process includes: selecting a suitable template molecule, functional monomer, crosslinking agent and initiator, forming a polymer matrix through a polymerization reaction, and removing the template molecule after polymerization to obtain a molecularly imprinted polymer with specific binding sites. S300. The pretreated industrial wastewater sample is brought into contact with the molecularly imprinted polymer to react, so that the target pollutants in the industrial wastewater sample are selectively adsorbed onto the binding sites of the molecularly imprinted polymer. S400: Separate the molecularly imprinted polymer and the target pollutants it adsorbs, and clean the molecularly imprinted polymer to remove unadsorbed interfering substances. S500. Detect the target pollutants adsorbed on the molecularly imprinted polymer, and analyze the content of the target pollutants in the industrial wastewater sample based on the detection results.
2. The rapid detection method for industrial wastewater pollutants based on molecularly imprinted polymers according to claim 1, characterized in that, The pretreatment of the industrial wastewater sample in S100 includes at least one of the following: filtration, centrifugation, pH adjustment, solid-phase extraction or liquid-liquid extraction.
3. The rapid detection method for industrial wastewater pollutants based on molecularly imprinted polymers according to claim 1, characterized in that, The specific process for preparing the molecularly imprinted polymer in S200 includes: S210. The template molecule, functional monomer, crosslinking agent and initiator are added to the solvent in a preset molar ratio to form a prepolymerization mixture; S220. The prepolymer mixture is subjected to a polymerization reaction to form a molecularly imprinted polymer matrix. The polymerization reaction may be carried out under light, heat or a specific catalyst. S230. The obtained polymer matrix is washed and dried to remove unreacted monomers and impurities. S240. Remove the template molecules in the polymer matrix using a suitable eluent to obtain a molecularly imprinted polymer capable of recognizing target pollutants, wherein the eluent is selected from methanol, ethanol, acetonitrile, acetone, dimethylformamide, acetic acid, or mixtures thereof.
4. The rapid detection method for industrial wastewater pollutants based on molecularly imprinted polymers according to claim 3, characterized in that, The functional monomer is selected from at least one of acrylamide, methacrylic acid, vinylpyridine, styrene, acrylic acid, isopropylacrylamide, 2-hydroxyethyl methacrylate, vinyl acetate, or their derivatives; the crosslinking agent is selected from at least one of ethylene glycol dimethacrylate, N,N'-methylenebisacrylamide, trimethylolpropane trimethacrylate, divinylbenzene, or their derivatives; the initiator is selected from at least one of benzoyl peroxide, azobisisobutyronitrile, or potassium persulfate; and the solvent is selected from at least one of methanol, ethanol, acetone, acetonitrile, chloroform, dimethylformamide, water, or mixtures thereof.
5. The rapid detection method for industrial wastewater pollutants based on molecularly imprinted polymers according to claim 1, characterized in that, In step S300, the pretreated industrial wastewater sample is contacted with the molecularly imprinted polymer in at least one of the following methods: oscillation adsorption, stirring adsorption, column adsorption, or fluid adsorption through a membrane.
6. The rapid detection method for industrial wastewater pollutants based on molecularly imprinted polymers according to claim 1, characterized in that, In step S400, the molecularly imprinted polymer and its adsorbed target pollutants are separated by centrifugation, filtration, or magnetic separation. The molecularly imprinted polymer is cleaned using pure water, buffer solution, low-concentration eluent, or a combination thereof to ensure the removal of non-specifically bound substances.
7. The rapid detection method for industrial wastewater pollutants based on molecularly imprinted polymers according to claim 1, characterized in that, The target pollutant detected in step S500 on the molecularly imprinted polymer includes at least one of the following: S510. Directly perform physical or chemical signal detection on the molecularly imprinted polymer adsorbed with the target pollutant, wherein the detection method includes electrochemical analysis, spectroscopic analysis, chromatography or mass spectrometry. S520. The adsorbed target pollutant is eluted from the molecularly imprinted polymer, and then the target pollutant in the eluent is detected. The detection method includes high performance liquid chromatography, gas chromatography, gas chromatography-mass spectrometry, liquid chromatography-mass spectrometry, atomic absorption spectrometry, inductively coupled plasma atomic emission spectrometry, or ultraviolet-visible spectrophotometry.
8. The rapid detection method for industrial wastewater pollutants based on molecularly imprinted polymers according to claim 1, characterized in that, The target pollutant is selected from at least one of heavy metal ions, phenolic compounds, aniline compounds, polycyclic aromatic hydrocarbons, pesticide residues, antibiotics, plasticizers, or endocrine disruptors.
9. The rapid detection method for industrial wastewater pollutants based on molecularly imprinted polymers according to claim 1, characterized in that, The molecularly imprinted polymer exists in the form of microspheres, nanoparticles, membranes, thin films, or modified electrodes to improve adsorption efficiency and detection response speed.
10. The rapid detection method for industrial wastewater pollutants based on molecularly imprinted polymers according to claim 9, characterized in that, The method further includes: after S500, regenerating the molecularly imprinted polymer to make it reusable, the regeneration process including eluting the adsorbed target pollutants with a suitable eluent and activating the polymer.