Malus spectabilis flowering phase extender and preparation method thereof

Abstract

The invention discloses a flowering phase extender for begonia and a preparation method of the flowering phase extender, and belongs to the technical field of plant growth regulators. The cape jasmine flower preservative is prepared from the following raw materials in parts by weight: 0.02 to 0.04 part of alpha-naphthylacetic acid, 0.1 to 0.15 part of salicylic acid, 0.1 to 0.3 part of glutamic acid, 5 to 8 parts of cape jasmine flower extract, 3 to 5 parts of accelerant, 1 to 1.2 parts of mannitol and 100 to 120 parts of water. The accelerant is composed of a soybean protein isolate-seaweed extract compound and sodium lauroyl taurate according to the mass ratio of 5: 1. Through the synergistic effect of multiple active ingredients, the flowering phase of the Chinese flowering apple can be remarkably prolonged, meanwhile, healthy growth of plants is promoted, and stress resistance is enhanced.

Description

Technical Field

[0001] This invention belongs to the field of plant growth regulator technology, specifically relating to a flowering period prolonging agent for crabapple flowers and its preparation method. Background Technology

[0002] During the flowering period, plants transition from vegetative growth to reproductive growth. The development of a flower is influenced by both its own genetic factors and environmental factors. The flowering period of flowering plants involves the process from blooming to withering, signifying the formation, development, maturation, and senescence of the floral organs. During senescence, insufficient nutrient supply within the floral organs leads to a decline in physiological metabolism. The activity of antioxidant enzymes and osmotic regulators becomes insufficient to prevent tissue damage caused by reactive oxygen species, resulting in cell membrane rupture and cytoplasmic destruction, ultimately ending the flowering period. Premature senescence causes the floral organs to wither and die rapidly, hindering pollination and reproduction, and significantly reducing ornamental value and landscape appeal. With the development of science and technology and the improvement of people's living standards, the concept of ecological construction has become deeply ingrained. Flowering plants are widely used worldwide, from courtyards, communities, and parks to cities, from coastlines, wetlands, and plains to mountains; the demand for flowering landscapes has never ceased. Inhibiting the senescence process of flowering plant floral organs, maintaining the flowering period, and ensuring a good ornamental effect for a certain period have become increasingly important scientific issues.

[0003] The flowering period of crabapple blossoms, a flower of high ornamental value, directly impacts its aesthetic appeal. However, due to factors such as environment, climate, and the plant's own physiological characteristics, the flowering period of crabapple blossoms is often relatively short. While some flowering period extenders exist on the market, those specifically designed for crabapple blossoms are rare, and their effectiveness needs improvement. Therefore, developing a flowering period extender specifically for crabapple blossoms to prolong their flowering period and enhance their ornamental value is of great significance. Summary of the Invention

[0004] The purpose of this invention is to provide a flowering period prolonging agent for crabapple flowers. This agent, through the synergistic effect of multiple active ingredients, can significantly prolong the flowering period of crabapple flowers, while promoting healthy plant growth and enhancing stress resistance.

[0005] To achieve the above-mentioned technical objectives, the technical solution adopted by the present invention is as follows: A flowering period prolonging agent for crabapple blossoms is made from the following raw materials in parts by weight: 0.02-0.04 parts α-naphthaleneacetic acid, 0.1-0.15 parts salicylic acid, 0.1-0.3 parts glutamic acid, 5-8 parts gardenia extract, 3-5 parts promoter, 1-1.2 parts mannitol, and 100-120 parts water; wherein the promoter is composed of a soybean protein isolate-seaweed extract complex and sodium lauroyl taurate in a mass ratio of 5:1.

[0006] Preferably, the gardenia extract is prepared by the following method: (1) Wash the fresh gardenia flowers, dry them at 60°C to constant weight, and pulverize them through a 60-mesh sieve to obtain gardenia flower powder; (2) Gardenia pollen was added to anhydrous ethanol at a solid-liquid ratio and heated to 65°C for 2 hours. The filtrate was then filtered. The filtrate was extracted twice and combined. The extract was concentrated under reduced pressure and dried at low temperature to obtain gardenia extract.

[0007] Preferably, the solid-liquid ratio in step (2) is 1g:20ml.

[0008] Preferably, the soy protein isolate-seaweed extract complex is prepared by the following method: Step 1: Take fresh brown algae, wash and dry it, then crush it and pass it through an 80-mesh sieve to obtain brown algae powder; Step 2: Mix brown algae powder with deionized water at a ratio of 1g:10ml, adjust the pH of the mixture to 5.5-6.0, add compound enzyme, and enzymatically hydrolyze at 50-55℃ for 4 hours. After enzyme inactivation, obtain the enzymatic hydrolysate. Step 3: Centrifuge the enzymatic hydrolysate obtained in Step 2 to obtain the supernatant. Add 3 times the volume of anhydrous ethanol to the supernatant, let it stand at 4°C for 8-12 hours, then centrifuge to obtain the seaweed extract. Step 4: Dissolve soy protein isolate in deionized water at a solid-liquid ratio of 1g:50ml, sonicate for 20min, then transfer to low-temperature plasma treatment, and finally freeze-dry to obtain pretreated soy protein isolate powder. Step 5: Dissolve the pretreated soy protein isolate powder and the seaweed extract obtained in Step 3 in deionized water to obtain a pretreated soy protein isolate solution and a seaweed extract solution. Mix the two solutions in volume ratio, adjust the pH of the mixture to 8.0-8.5, stir magnetically and heat to 60°C for 4-6 hours. After the reaction is completed, quickly cool to room temperature and adjust the mixture to neutral. Freeze-dry to obtain the soy protein isolate-seaweed extract complex.

[0009] Preferably, the amount of compound enzyme added in step 2 is 2% of the dry weight of brown algae powder.

[0010] Preferably, the complex enzyme is composed of cellulase, papain, β-glucosidase, and xylanase in a mass ratio of 1:0.1:0.5:0.2-0.3.

[0011] Preferably, the cellulase has an enzyme activity of 100,000 u / g, the papain has an enzyme activity of 100,000 u / g, the β-glucosidase has an enzyme activity of 1,000 u / ml, and the xylanase has an enzyme activity of 50,000 u / g.

[0012] Preferably, the ultrasonic treatment power in step 4 is 300W; the low-temperature plasma treatment time is 5min and the power is 300W.

[0013] Preferably, in step 5, the mass fraction of the pretreated soy protein isolate solution is 2%, and the mass fraction of the seaweed extract solution is 5%; the volume ratio of the pretreated soy protein isolate solution to the seaweed extract solution is 1:2.

[0014] This invention also provides a method for preparing the above-mentioned crabapple blossom extension agent, comprising the following steps: S1, Preparation of gardenia extract; S2, prepare soy protein isolate-seaweed extract complex, and combine it with sodium lauroyl taurate in a certain proportion to form an accelerator; S3 is obtained by mixing gardenia extract, accelerator and other remaining raw materials in proportion.

[0015] The flowering period extender for crabapple flowers prepared in this invention can effectively extend the flowering period of various crabapple species commonly used in landscaping, such as Malus halliana, Malus spectabilis, and Chaenomeles speciosa.

[0016] The beneficial effects of this invention are: (1) This invention uses a combination of ultrasonic and low-temperature plasma treatment to combine soy protein isolate with seaweed extract. By optimizing the glycosylation reaction conditions, the hydrophobicity of soy protein isolate is reduced, and the water solubility and stability of soy protein isolate are improved. The resulting product has slow-release properties and can form a protective film on the plant surface, reducing water evaporation and external stress damage while slowly releasing active ingredients and prolonging the action time. At the same time, soy protein isolate is rich in amino acids, which can provide nitrogen source and nutritional support for plants, promoting plant growth and flowering. Seaweed extract is rich in polysaccharides, minerals, etc., which can enhance the plant's stress resistance. Sodium lauroyl taurate can improve the adhesion and permeability of the complex, enhance the absorption efficiency of active ingredients, and when combined with soy protein isolate-seaweed extract complex in a specific ratio, a stable promoter system is formed, which further enhances the effect of prolonging the flowering period.

[0017] (2) Gardenia extract contains various bioactive components such as polysaccharides, phenols, flavonoids, and total iridoids, which can scavenge free radicals in plants, reduce oxidative damage during flower senescence, maintain flower vibrancy, and prolong the flowering period. Simultaneously, the iridoids in gardenia extract have anti-inflammatory and anti-disease effects, protecting flowers from pathogens and extending the flowering period. When used in combination with other components in the formulation of this invention, it can significantly prolong the flowering period of crabapple blossoms, with remarkable effects.

[0018] (3) α-Naphthaleneacetic acid is a common plant growth regulator. When used alone, it has a poor effect on prolonging the flowering period of crabapple. After being used in combination with gardenia extract, promoter and other ingredients prepared in this invention, the multiple ingredients work synergistically and the active ingredients are released for a long time. On the one hand, it promotes the growth of crabapple, reduces the invasion of diseases and improves the plant's resistance. On the other hand, it reduces the oxidative damage during the aging process of crabapple flowers, prolongs the flowering period and improves the ornamental value of crabapple flowers. Attached Figure Description

[0019] Figure 1 This is a comparison diagram of the growth of Malus spectabilis in Example 3 and the blank control group at the same time in Application Example 1 of the present invention, where A is the treatment group of Example 3 and B is the blank control treatment group; Figure 2 This is a comparison diagram of the growth of *Chaenomeles speciosa* in Example 3 and the blank control group at the same time in Application Example 2 of the present invention, where A is the blank control group and B is the Example 3 treatment group. Detailed Implementation

[0020] The technical solution of the present invention will be further described below with reference to specific embodiments, but it is not limited thereto.

[0021] Example 1 A flowering period prolonging agent for crabapple blossoms is made from the following raw materials in parts by weight: 0.04 parts α-naphthaleneacetic acid, 0.15 parts salicylic acid, 0.3 parts glutamic acid, 8 parts gardenia extract, 5 parts accelerator, 1.2 parts mannitol, and 120 parts water; wherein the accelerator is composed of a soybean protein isolate-seaweed extract complex and sodium lauroyl taurate in a mass ratio of 5:1.

[0022] The gardenia extract was prepared using the following method: (1) Wash the fresh gardenia flowers, dry them at 60°C to constant weight, and pulverize them through a 60-mesh sieve to obtain gardenia flower powder; (2) Gardenia pollen was added to anhydrous ethanol at a solid-liquid ratio of 1g:20ml and heated to 65℃ for 2h. The filtrate was then filtered. The filtrate was extracted twice and combined. The extract was concentrated under reduced pressure and dried at low temperature to obtain gardenia extract.

[0023] The soybean protein isolate-seaweed extract complex was prepared using the following method: Step 1: Take fresh brown algae, wash and dry it, then crush it and pass it through an 80-mesh sieve to obtain brown algae powder; Step 2: Mix brown algae powder with deionized water at a ratio of 1g:10ml, adjust the pH of the mixture to 5.5-6.0, add a compound enzyme accounting for 2% of the dry weight of the brown algae powder, and enzymatically hydrolyze at 50-55℃ for 4 hours. After enzyme inactivation, obtain the enzymatic hydrolysate. The compound enzyme is composed of cellulase, papain, β-glucosidase, and xylanase in a mass ratio of 1:0.1:0.5:0.2-0.3. The cellulase has an enzyme activity of 100,000 u / g, the papain has an enzyme activity of 100,000 u / g, the β-glucosidase has an enzyme activity of 1,000 u / ml, and the xylanase has an enzyme activity of 50,000 u / g. Step 3: Centrifuge the enzymatic hydrolysate obtained in Step 2 to obtain the supernatant. Add 3 times the volume of anhydrous ethanol to the supernatant, let it stand at 4°C for 8-12 hours, then centrifuge to obtain the seaweed extract. Step 4: Dissolve soy protein isolate in deionized water at a solid-liquid ratio of 1g:50ml, sonicate for 20min, then transfer to low-temperature plasma treatment, and finally freeze-dry to obtain pretreated soy protein isolate powder. Step 5: Dissolve the pretreated soy protein isolate powder and the seaweed extract obtained in Step 3 in deionized water to obtain a pretreated soy protein isolate solution and a seaweed extract solution. Mix the two solutions in volume ratio, adjust the pH of the mixture to 8.0-8.5, stir magnetically and heat to 60°C for 4-6 hours. After the reaction is completed, quickly cool to room temperature and adjust the mixture to neutral. Freeze-dry to obtain the soy protein isolate-seaweed extract complex.

[0024] In step 4, the ultrasonic treatment power is 300W; the low-temperature plasma treatment time is 5 minutes and the power is 300W.

[0025] In step 5, the mass fraction of the pretreated soy protein isolate solution is 2%, and the mass fraction of the seaweed extract solution is 5%; the volume ratio of the pretreated soy protein isolate solution to the seaweed extract solution is 1:2.

[0026] A method for preparing the above-mentioned crabapple blossom extension agent includes the following steps: S1, Preparation of gardenia extract; S2, prepare soy protein isolate-seaweed extract complex, and combine it with sodium lauroyl taurate in a certain proportion to form an accelerator; S3 is obtained by mixing gardenia extract, accelerator and other remaining raw materials in proportion.

[0027] Example 2 A flowering period prolonging agent for crabapple blossoms is made from the following raw materials in parts by weight: 0.02 parts α-naphthaleneacetic acid, 0.1 parts salicylic acid, 0.1 parts glutamic acid, 5 parts gardenia extract, 3 parts promoter, 1 part mannitol, and 100 parts water; wherein the promoter is composed of a soybean protein isolate-seaweed extract complex and sodium lauroyl taurate in a mass ratio of 5:1.

[0028] The gardenia extract was prepared using the following method: (1) Wash the fresh gardenia flowers, dry them at 60°C to constant weight, and pulverize them through a 60-mesh sieve to obtain gardenia flower powder; (2) Gardenia pollen was added to anhydrous ethanol at a solid-liquid ratio of 1g:20ml and heated to 65℃ for 2h. The filtrate was then filtered. The filtrate was extracted twice and combined. The extract was concentrated under reduced pressure and dried at low temperature to obtain gardenia extract.

[0029] The soybean protein isolate-seaweed extract complex was prepared using the following method: Step 1: Take fresh brown algae, wash and dry it, then crush it and pass it through an 80-mesh sieve to obtain brown algae powder; Step 2: Mix brown algae powder with deionized water at a ratio of 1g:10ml, adjust the pH of the mixture to 5.5-6.0, add a compound enzyme accounting for 2% of the dry weight of the brown algae powder, and enzymatically hydrolyze at 50-55℃ for 4 hours. After enzyme inactivation, obtain the enzymatic hydrolysate. The compound enzyme is composed of cellulase, papain, β-glucosidase, and xylanase in a mass ratio of 1:0.1:0.5:0.2-0.3. The cellulase has an enzyme activity of 100,000 u / g, the papain has an enzyme activity of 100,000 u / g, the β-glucosidase has an enzyme activity of 1,000 u / ml, and the xylanase has an enzyme activity of 50,000 u / g. Step 3: Centrifuge the enzymatic hydrolysate obtained in Step 2 to obtain the supernatant. Add 3 times the volume of anhydrous ethanol to the supernatant, let it stand at 4°C for 8-12 hours, then centrifuge to obtain the seaweed extract. Step 4: Dissolve soy protein isolate in deionized water at a solid-liquid ratio of 1g:50ml, sonicate for 20min, then transfer to low-temperature plasma treatment, and finally freeze-dry to obtain pretreated soy protein isolate powder. Step 5: Dissolve the pretreated soy protein isolate powder and the seaweed extract obtained in Step 3 in deionized water to obtain a pretreated soy protein isolate solution and a seaweed extract solution. Mix the two solutions in volume ratio, adjust the pH of the mixture to 8.0-8.5, stir magnetically and heat to 60°C for 4-6 hours. After the reaction is completed, quickly cool to room temperature and adjust the mixture to neutral. Freeze-dry to obtain the soy protein isolate-seaweed extract complex.

[0030] In step 4, the ultrasonic treatment power is 300W; the low-temperature plasma treatment time is 5 minutes and the power is 300W.

[0031] In step 5, the mass fraction of the pretreated soy protein isolate solution is 2%, and the mass fraction of the seaweed extract solution is 5%; the volume ratio of the pretreated soy protein isolate solution to the seaweed extract solution is 1:2.

[0032] A method for preparing the above-mentioned crabapple blossom extension agent includes the following steps: S1, Preparation of gardenia extract; S2, prepare soy protein isolate-seaweed extract complex, and combine it with sodium lauroyl taurate in a certain proportion to form an accelerator; S3 is obtained by mixing gardenia extract, accelerator and other remaining raw materials in proportion.

[0033] Example 3 A flowering period prolonging agent for crabapple blossoms is made from the following raw materials in parts by weight: 0.03 parts α-naphthaleneacetic acid, 0.12 parts salicylic acid, 0.2 parts glutamic acid, 7 parts gardenia extract, 4 parts accelerator, 1.1 parts mannitol, and 110 parts water; wherein the accelerator is composed of a soybean protein isolate-seaweed extract complex and sodium lauroyl taurate in a mass ratio of 5:1.

[0034] The gardenia extract was prepared using the following method: (1) Wash the fresh gardenia flowers, dry them at 60°C to constant weight, and pulverize them through a 60-mesh sieve to obtain gardenia flower powder; (2) Gardenia pollen was added to anhydrous ethanol at a solid-liquid ratio of 1g:20ml and heated to 65℃ for 2h. The filtrate was then filtered. The filtrate was extracted twice and combined. The extract was concentrated under reduced pressure and dried at low temperature to obtain gardenia extract.

[0035] The soybean protein isolate-seaweed extract complex was prepared using the following method: Step 1: Take fresh brown algae, wash and dry it, then crush it and pass it through an 80-mesh sieve to obtain brown algae powder; Step 2: Mix brown algae powder with deionized water at a ratio of 1g:10ml, adjust the pH of the mixture to 5.5-6.0, add a compound enzyme accounting for 2% of the dry weight of the brown algae powder, and enzymatically hydrolyze at 50-55℃ for 4 hours. After enzyme inactivation, obtain the enzymatic hydrolysate. The compound enzyme is composed of cellulase, papain, β-glucosidase, and xylanase in a mass ratio of 1:0.1:0.5:0.2-0.3. The cellulase has an enzyme activity of 100,000 u / g, the papain has an enzyme activity of 100,000 u / g, the β-glucosidase has an enzyme activity of 1,000 u / ml, and the xylanase has an enzyme activity of 50,000 u / g. Step 3: Centrifuge the enzymatic hydrolysate obtained in Step 2 to obtain the supernatant. Add 3 times the volume of anhydrous ethanol to the supernatant, let it stand at 4°C for 8-12 hours, then centrifuge to obtain the seaweed extract. Step 4: Dissolve soy protein isolate in deionized water at a solid-liquid ratio of 1g:50ml, sonicate for 20min, then transfer to low-temperature plasma treatment, and finally freeze-dry to obtain pretreated soy protein isolate powder. Step 5: Dissolve the pretreated soy protein isolate powder and the seaweed extract obtained in Step 3 in deionized water to obtain a pretreated soy protein isolate solution and a seaweed extract solution. Mix the two solutions in volume ratio, adjust the pH of the mixture to 8.0-8.5, stir magnetically and heat to 60°C for 4-6 hours. After the reaction is completed, quickly cool to room temperature and adjust the mixture to neutral. Freeze-dry to obtain the soy protein isolate-seaweed extract complex.

[0036] In step 4, the ultrasonic treatment power is 300W; the low-temperature plasma treatment time is 5 minutes and the power is 300W.

[0037] In step 5, the mass fraction of the pretreated soy protein isolate solution is 2%, and the mass fraction of the seaweed extract solution is 5%; the volume ratio of the pretreated soy protein isolate solution to the seaweed extract solution is 1:2.

[0038] A method for preparing the above-mentioned crabapple blossom extension agent includes the following steps: S1, Preparation of gardenia extract; S2, prepare soy protein isolate-seaweed extract complex, and combine it with sodium lauroyl taurate in a certain proportion to form an accelerator; S3 is obtained by mixing gardenia extract, accelerator and other remaining raw materials in proportion.

[0039] Comparative Example 1 A flowering period prolonging agent for crabapple blossoms is made from the following raw materials in parts by weight: 0.03 parts α-naphthaleneacetic acid, 0.12 parts salicylic acid, 0.2 parts glutamic acid, 7 parts gardenia extract, 4 parts accelerator, 1.1 parts mannitol, and 110 parts water; wherein the accelerator is composed of a soybean protein isolate-seaweed extract complex and sodium lauroyl taurate in a mass ratio of 5:1.

[0040] The soybean protein isolate-seaweed extract complex was prepared using the following method: Step 1: Take fresh brown algae, wash and dry it, then crush it and pass it through an 80-mesh sieve to obtain brown algae powder; Step 2: Mix brown algae powder with deionized water at a ratio of 1g:10ml, adjust the pH of the mixture to 5.5-6.0, add a compound enzyme accounting for 2% of the dry weight of the brown algae powder, and enzymatically hydrolyze at 50-55℃ for 4 hours. After enzyme inactivation, obtain the enzymatic hydrolysate. The compound enzyme is composed of cellulase, papain, β-glucosidase, and xylanase in a mass ratio of 1:0.1:0.5:0.2-0.3. The cellulase has an enzyme activity of 100,000 u / g, the papain has an enzyme activity of 100,000 u / g, the β-glucosidase has an enzyme activity of 1,000 u / ml, and the xylanase has an enzyme activity of 50,000 u / g. Step 3: Centrifuge the enzymatic hydrolysate obtained in Step 2 to obtain the supernatant. Add 3 times the volume of anhydrous ethanol to the supernatant, let it stand at 4°C for 8-12 hours, then centrifuge to obtain the seaweed extract. Step 4: Dissolve soy protein isolate in deionized water at a solid-liquid ratio of 1g:50ml, sonicate for 20min, then transfer to low-temperature plasma treatment, and finally freeze-dry to obtain pretreated soy protein isolate powder. Step 5: Dissolve the pretreated soy protein isolate powder and the seaweed extract obtained in Step 3 in deionized water to obtain a pretreated soy protein isolate solution and a seaweed extract solution. Mix the two solutions in volume ratio, adjust the pH of the mixture to 8.0-8.5, stir magnetically and heat to 60°C for 4-6 hours. After the reaction is completed, quickly cool to room temperature and adjust the mixture to neutral. Freeze-dry to obtain the soy protein isolate-seaweed extract complex.

[0041] In step 4, the ultrasonic treatment power is 300W; the low-temperature plasma treatment time is 5 minutes and the power is 300W.

[0042] In step 5, the mass fraction of the pretreated soy protein isolate solution is 2%, and the mass fraction of the seaweed extract solution is 5%; the volume ratio of the pretreated soy protein isolate solution to the seaweed extract solution is 1:2.

[0043] A method for preparing the above-mentioned crabapple blossom extension agent includes the following steps: S1, prepare a soybean protein isolate-seaweed extract complex, and combine it with sodium lauroyl taurate in a certain proportion to form an accelerator; S2 is obtained by mixing the accelerator with other remaining raw materials in a certain proportion.

[0044] This comparative example is identical to Example 3 except that it does not contain gardenia extract.

[0045] Comparative Example 2 A flowering period prolonging agent for crabapple blossoms is made from the following raw materials in parts by weight: 0.03 parts α-naphthaleneacetic acid, 0.12 parts salicylic acid, 0.2 parts glutamic acid, 7 parts gardenia extract, 1.1 parts mannitol, and 110 parts water. The gardenia extract is prepared by the following method: (1) Wash the fresh gardenia flowers, dry them at 60°C to constant weight, and pulverize them through a 60-mesh sieve to obtain gardenia flower powder; (2) Gardenia pollen was added to anhydrous ethanol at a solid-liquid ratio of 1g:20ml and heated to 65℃ for 2h. The filtrate was then filtered. The filtrate was extracted twice and combined. The extract was concentrated under reduced pressure and dried at low temperature to obtain gardenia extract.

[0046] A method for preparing the above-mentioned crabapple blossom extension agent includes the following steps: S1, Preparation of gardenia extract; S2, the gardenia extract is mixed evenly with the other remaining ingredients in a certain proportion.

[0047] This comparative example is identical to Example 3 except that it does not contain an accelerator.

[0048] Comparative Example 3 A flowering period prolonging agent for crabapple blossoms is made from the following raw materials in parts by weight: 0.03 parts α-naphthaleneacetic acid, 0.12 parts salicylic acid, 0.2 parts glutamic acid, 7 parts gardenia extract, 4 parts accelerator, 1.1 parts mannitol, and 110 parts water; wherein the accelerator is composed of a soybean protein isolate-seaweed extract complex and sodium lauroyl taurate in a mass ratio of 4:1.

[0049] The gardenia extract was prepared using the following method: (1) Wash the fresh gardenia flowers, dry them at 60°C to constant weight, and pulverize them through a 60-mesh sieve to obtain gardenia flower powder; (2) Gardenia pollen was added to anhydrous ethanol at a solid-liquid ratio of 1g:20ml and heated to 65℃ for 2h. The filtrate was then filtered. The filtrate was extracted twice and combined. The extract was concentrated under reduced pressure and dried at low temperature to obtain gardenia extract.

[0050] The soybean protein isolate-seaweed extract complex was prepared using the following method: Step 1: Take fresh brown algae, wash and dry it, then crush it and pass it through an 80-mesh sieve to obtain brown algae powder; Step 2: Mix brown algae powder with deionized water at a ratio of 1g:10ml, adjust the pH of the mixture to 5.5-6.0, add a compound enzyme accounting for 2% of the dry weight of the brown algae powder, and enzymatically hydrolyze at 50-55℃ for 4 hours. After enzyme inactivation, obtain the enzymatic hydrolysate. The compound enzyme is composed of cellulase, papain, β-glucosidase, and xylanase in a mass ratio of 1:0.1:0.5:0.2-0.3. The cellulase has an enzyme activity of 100,000 u / g, the papain has an enzyme activity of 100,000 u / g, the β-glucosidase has an enzyme activity of 1,000 u / ml, and the xylanase has an enzyme activity of 50,000 u / g. Step 3: Centrifuge the enzymatic hydrolysate obtained in Step 2 to obtain the supernatant. Add 3 times the volume of anhydrous ethanol to the supernatant, let it stand at 4°C for 8-12 hours, then centrifuge to obtain the seaweed extract. Step 4: Dissolve soy protein isolate in deionized water at a solid-liquid ratio of 1g:50ml, sonicate for 20min, then transfer to low-temperature plasma treatment, and finally freeze-dry to obtain pretreated soy protein isolate powder. Step 5: Dissolve the pretreated soy protein isolate powder and the seaweed extract obtained in Step 3 in deionized water to obtain a pretreated soy protein isolate solution and a seaweed extract solution. Mix the two solutions in volume ratio, adjust the pH of the mixture to 8.0-8.5, stir magnetically and heat to 60°C for 4-6 hours. After the reaction is completed, quickly cool to room temperature and adjust the mixture to neutral. Freeze-dry to obtain the soy protein isolate-seaweed extract complex.

[0051] In step 4, the ultrasonic treatment power is 300W; the low-temperature plasma treatment time is 5 minutes and the power is 300W.

[0052] In step 5, the mass fraction of the pretreated soy protein isolate solution is 2%, and the mass fraction of the seaweed extract solution is 5%; the volume ratio of the pretreated soy protein isolate solution to the seaweed extract solution is 1:2.

[0053] A method for preparing the above-mentioned crabapple blossom extension agent includes the following steps: S1, Preparation of gardenia extract; S2, prepare soy protein isolate-seaweed extract complex, and combine it with sodium lauroyl taurate in a certain proportion to form an accelerator; S3 is obtained by mixing gardenia extract, accelerator and other remaining raw materials in proportion.

[0054] This comparative example is identical to Example 3 except for the different ratios of the two accelerators.

[0055] Comparative Example 4 A flowering period prolonging agent for crabapple blossoms is made from the following raw materials in parts by weight: 0.03 parts α-naphthaleneacetic acid, 0.12 parts salicylic acid, 0.2 parts glutamic acid, 7 parts gardenia extract, 4 parts accelerator, 1.1 parts mannitol, and 110 parts water; wherein the accelerator is composed of a soybean protein isolate-seaweed extract complex and sodium lauroyl taurate in a mass ratio of 6:1.

[0056] The gardenia extract was prepared using the following method: (1) Wash the fresh gardenia flowers, dry them at 60°C to constant weight, and pulverize them through a 60-mesh sieve to obtain gardenia flower powder; (2) Gardenia pollen was added to anhydrous ethanol at a solid-liquid ratio of 1g:20ml and heated to 65℃ for 2h. The filtrate was then filtered. The filtrate was extracted twice and combined. The extract was concentrated under reduced pressure and dried at low temperature to obtain gardenia extract.

[0057] The soybean protein isolate-seaweed extract complex was prepared using the following method: Step 1: Take fresh brown algae, wash and dry it, then crush it and pass it through an 80-mesh sieve to obtain brown algae powder; Step 2: Mix brown algae powder with deionized water at a ratio of 1g:10ml, adjust the pH of the mixture to 5.5-6.0, add a compound enzyme accounting for 2% of the dry weight of the brown algae powder, and enzymatically hydrolyze at 50-55℃ for 4 hours. After enzyme inactivation, obtain the enzymatic hydrolysate. The compound enzyme is composed of cellulase, papain, β-glucosidase, and xylanase in a mass ratio of 1:0.1:0.5:0.2-0.3. The cellulase has an enzyme activity of 100,000 u / g, the papain has an enzyme activity of 100,000 u / g, the β-glucosidase has an enzyme activity of 1,000 u / ml, and the xylanase has an enzyme activity of 50,000 u / g. Step 3: Centrifuge the enzymatic hydrolysate obtained in Step 2 to obtain the supernatant. Add 3 times the volume of anhydrous ethanol to the supernatant, let it stand at 4°C for 8-12 hours, then centrifuge to obtain the seaweed extract. Step 4: Dissolve soy protein isolate in deionized water at a solid-liquid ratio of 1g:50ml, sonicate for 20min, then transfer to low-temperature plasma treatment, and finally freeze-dry to obtain pretreated soy protein isolate powder. Step 5: Dissolve the pretreated soy protein isolate powder and the seaweed extract obtained in Step 3 in deionized water to obtain a pretreated soy protein isolate solution and a seaweed extract solution. Mix the two solutions in volume ratio, adjust the pH of the mixture to 8.0-8.5, stir magnetically and heat to 60°C for 4-6 hours. After the reaction is completed, quickly cool to room temperature and adjust the mixture to neutral. Freeze-dry to obtain the soy protein isolate-seaweed extract complex.

[0058] In step 4, the ultrasonic treatment power is 300W; the low-temperature plasma treatment time is 5 minutes and the power is 300W.

[0059] In step 5, the mass fraction of the pretreated soy protein isolate solution is 2%, and the mass fraction of the seaweed extract solution is 5%; the volume ratio of the pretreated soy protein isolate solution to the seaweed extract solution is 1:2.

[0060] A method for preparing the above-mentioned crabapple blossom extension agent includes the following steps: S1, Preparation of gardenia extract; S2, prepare soy protein isolate-seaweed extract complex, and combine it with sodium lauroyl taurate in a certain proportion to form an accelerator; S3 is obtained by mixing gardenia extract, accelerator and other remaining raw materials in proportion.

[0061] This comparative example is identical to Example 3 except for the different ratios of the two accelerators.

[0062] Application Example 1 Experimental Site: A crabapple plantation in a riverside park in Linyi City was selected as the experimental site. The crabapple variety was 3-year-old Malus spectabilis, with a plant height of 1.5-1.8 meters, and planted in multiple rows. Seven treatment groups were set up: Example 3, Comparative Examples 1-4, α-naphthaleneacetic acid treatment group, and blank control group, with 10 plants in each treatment group. During the budding and budding stages, the growth regulators obtained in Example 3 and Comparative Examples 1-4 were sprayed at a concentration of 10 ml / plant diluted 200 times. Crabapple flowers sprayed with α-naphthaleneacetic acid served as the control group, and crabapple flowers sprayed with an equal amount of water served as the blank control. Growth and flowering period changes were tracked and recorded. Starting from the swelling of the first flower bud on the plant, phenological observations of the flowering period were conducted daily from 7:00 to 9:00 AM. The flowering period was divided into four stages: initial flowering, early flowering, full bloom, and flower fall, based on the condition that more than half of the plant was in bloom. The recording standards are as follows: the initial flowering period is the time when 1% of the flowers are open; the early flowering period is the time when about 5% of the flowers are open; the peak flowering period is the time when 25-75% of the flowers are open; and the petal fall period is when more than 75% of the petals have fallen off, and the flowers gradually wither until they have all fallen. The peak flowering period lasts from the beginning to the end of the peak flowering period, and the viewing period is from the peak flowering period to the petal fall period.

[0063] Table 1. Effects of different treatment groups on flowering period of Malus spectabilis As can be seen from the data in Table 1 above, compared with the blank control group, the flowering period of Malus spectabilis was extended by half after using the flower-extending agent of the present invention; while the flowering period of the α-naphthaleneacetic acid control group was earlier and the flowering period was longer than that of the blank group; the flowering period of the treatment groups in Comparative Examples 1-3 was longer than that of the blank control group, but the effect of extending the flowering period was significantly lower than that in Example 3. This indicates that the proportions of the raw materials in the formulation of the present invention are reasonable and interact with each other. Changing the composition or proportion of any of the raw materials may have a negative impact on the plants and is not conducive to extending the flowering period. In addition, as Figure 1As shown, compared with crabapple plants at the same time (4.20), it can be seen from the shape of the flower clusters and the vigorous growth that the ornamental value of Malus spectabilis is significantly improved after using the crabapple flowering period extender of Example 3 of the present invention.

[0064] Application Example 2 Experimental Site: A landscaping seedling planting base in Linyi City was selected as the experimental site. The crabapple variety used was *Chaenomeles speciosa*. Crabapple seedlings with a height of 40-50 cm, stem diameter of 2-3 cm, and uniform growth were selected and planted in pots, one seedling per pot. Seven treatment groups were set up: Example 3, Comparative Examples 1-4, α-naphthaleneacetic acid treatment group, and blank control group, with 10 pots in each treatment group. During the budding and budding stages, the growth regulators obtained in Example 3 and Comparative Examples 1-4 were sprayed or watered at a concentration of 2 ml / plant diluted 200 times. Crabapples treated with α-naphthaleneacetic acid served as the control group, and crabapples sprayed with an equal amount of water served as the blank control. Growth and flowering changes were tracked and recorded. Starting from the swelling of the first flower bud, flowering phenological observations were conducted daily from 7:00 AM to 9:00 AM. The flowering period was divided into four stages: initial flowering, early flowering, full bloom, and flower fall, based on the condition that more than half of the plant was in bloom. The recording standards are as follows: the initial flowering period is the time when 1% of the flowers are open; the early flowering period is the time when about 5% of the flowers are open; the peak flowering period is the time when 25-75% of the flowers are open; and the petal fall period is when more than 75% of the petals have fallen off, and the flowers gradually wither until they have all fallen. The peak flowering period lasts from the beginning to the end of the peak flowering period, and the viewing period is from the peak flowering period to the petal fall period.

[0065] Table 2 Effects of different treatment groups on flowering period of Chaenomeles speciosa As can be seen from the results in Table 2 above, after using the crabapple flowering period extender of Example 3 of the present invention, the viewing period was extended by 25 days compared with the blank control group and by 16 days compared with the α-naphthaleneacetic acid control group. While the flowering period of the treatment groups in Comparative Examples 1-4 was extended compared with the blank group, the effect was significantly lower than that of the treatment group in Example 3. This is because the present invention contains multiple components such as gardenia extract and promoters, which work synergistically to release active ingredients over a long period. On the one hand, this promotes crabapple growth, reduces disease invasion, and improves the plant's resistance; on the other hand, it reduces oxidative damage during the senescence of crabapple flowers, thus extending the flowering period and enhancing the ornamental value of crabapple flowers. The data from Comparative Examples 3-4 show that the promoter ratio of the present invention can fully utilize the nutritional and physiological regulatory effects of the soybean protein isolate-seaweed extract complex and also utilize the auxiliary penetration function of sodium lauroyl taurate to synergistically extend the flowering period. If the ratio is unbalanced, it will have a negative impact on the plant and will be detrimental to extending the flowering period.

[0066] At the end of March, one potted crabapple was randomly selected from the treatment group of Example 3 and the blank control group to observe its growth. Figure 2 As shown, compared with the blank control group, the flowering quince treated in Example 3 of this invention exhibited significantly better growth, which also demonstrates the good growth-promoting effect of the flowering period extender of this invention. Similarly, applying the flowering period extender of this invention to Malus halliana can also significantly extend the viewing period of Malus halliana.

[0067] It should be noted that the above embodiments are merely some preferred embodiments of the present invention, and not all embodiments. Obviously, based on the above embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort should fall within the scope of protection of the present invention.

Claims

1. A flower phase extender for Malus hupehensis, characterized by, It is made from the following raw materials by weight: α-naphthalene acetic acid 0.02-0.04 parts, salicylic acid 0.1-0.15 parts, glutamic acid 0.1-0.3 parts, gardenia flower extract 5-8 parts, accelerant 3-5 parts, mannitol 1-1.2 parts, water 100-120 parts; the accelerant is a soybean protein isolate-seaweed extract compound composed of soybean protein isolate and sodium lauroyl taurate according to a mass ratio of 5:

1.

2. The Malus toringo flower phase extender according to claim 1, characterized in that, The gardenia flower extract is prepared by the following method: (1) Wash fresh gardenia flowers, dry at 60°C until constant weight, and crush to pass through a 60-mesh sieve to obtain gardenia flower powder; (2) Take the gardenia flower powder, add solvent anhydrous ethanol according to a solid-liquid ratio, and heat to 65°C for 2h of extraction, then filter to obtain the filtrate; extract twice, combine the two filtrates, concentrate under reduced pressure, and dry at low temperature to obtain the gardenia flower extract.

3. The Malus hupehensis flowering period extender according to claim 2, characterized in that, The solid-liquid ratio in step (2) is 1g:20ml.

4. The Malus toringo flower phase extender according to claim 1, characterized in that, The soybean protein isolate-seaweed extract compound is prepared by the following method: Step 1, wash and dry fresh brown algae, crush, and pass through an 80-mesh sieve to obtain brown algae powder; Step 2, mix the brown algae powder with deionized water according to a ratio of 1g:10ml, adjust the pH of the mixture to 5.5-6.0, add a compound enzyme, and enzymatically hydrolyze at 50-55°C for 4h, then inactivate the enzyme to obtain an enzymatic hydrolysate; Step 3, centrifuge the enzymatic hydrolysate obtained in step 2 to obtain the supernatant, add 3 volumes of anhydrous ethanol to the supernatant, stand at 4°C for 8-12h, then centrifuge to obtain a precipitate, and take the precipitate to obtain a seaweed extract; Step 4, dissolve soybean protein isolate in deionized water according to a solid-liquid ratio of 1g:50ml, ultrasonically treat for 20min, then perform low-temperature plasma treatment, and freeze-dry to obtain pretreated soybean protein isolate powder; Step 5, dissolve the pretreated soybean protein isolate powder and the seaweed extract obtained in step 3 in deionized water to obtain a pretreated soybean protein isolate solution and a seaweed extract solution, respectively, mix the two solutions according to a volume ratio, adjust the pH of the mixture to 8.0-8.5, magnetically stir and heat to 60°C for 4-6h of reaction, then rapidly cool to room temperature, adjust the mixture to neutral, and freeze-dry to obtain a soybean protein isolate-seaweed extract compound.

5. The Malus toringo flower phase extender according to claim 4, characterized in that, The compound enzyme addition amount in step 2 is 2% of the dry weight of the brown algae powder.

6. The Malus toringo flower phase extender according to claim 5, characterized in that, The compound enzyme is composed of cellulase, papain, β-glucosidase, and xylanase according to a mass ratio of 1:0.1:0.5:0.2-0.

3.

7. The Malus toringo flower phase extender according to claim 6, characterized in that, The enzyme activity of the cellulase is 100,000u / g, the enzyme activity of the papain is 100,000u / g, the enzyme activity of the β-glucosidase is 1000u / ml, and the enzyme activity of the xylanase is 500,000u / g.

8. The Malus toringo flower phase extender according to claim 4, characterized in that, The ultrasonic treatment power in step 4 is 300W; the low-temperature plasma treatment time is 5min, and the power is 300W.

9. The Malus toringo flower phase extender according to claim 4, characterized in that, The mass fraction of the pretreated soybean protein isolate solution in step 5 is 2%, and the mass fraction of the seaweed extract solution is 5%; the volume ratio of the pretreated soybean protein isolate solution to the seaweed extract solution is 1:

2.

10. A method for preparing the Malus hupehensis flowering period extender according to any one of claims 1 to 9, characterized by, The following steps are included: S1, prepare a gardenia flower extract; S2, preparing soybean protein isolate-algal extract complex, and proportioning with sodium lauroyl taurate to form a promoter; S3, mixing gardenia flower extract, the promoter and other remaining raw materials in proportion to obtain.

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