Method for repairing wounds of tree stems

Abstract

The invention relates to the technical field of garden maintenance, and discloses a method for repairing wounds of tree stems. The repairing method comprises the following steps: S1, a root system induction stage: firstly carrying out cleaning, disinfection and preservative treatment on a wound surface, then obliquely cutting the upper edge of the wound surface to expose a cambium, smearing a root system inducer on an incision, then covering a peat soil matrix, and carrying out sealing, moisturizing and shading maintenance; s2, a root system traction stage, wherein adventitious roots are guided to the ground through a traction pipe, and water and fertilizer management is conducted; s3, a root system lignification promoting stage: promoting root system lignification through illumination control, exogenous chemical regulation and periodic water control. According to the repairing method, tree stem wound bark is induced to rapidly generate adventitious roots, a root system is introduced into the ground through a traction pipe and water and fertilizer induction, the nutrient transportation function of the bark is recovered through illumination control, exogenous chemical regulation and control, periodic water control and other measures, the tree is made to recover the normal growth vigor, operation is easy, environment adaptability is high, universality is high, and the method is suitable for large-scale popularization and application. The survival rate is high.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of garden maintenance, in particular to a method for repairing a wound on a tree stem. BACKGROUND

[0002] The bark of a tree is a protective barrier that defends against pests, mechanical damage, and extreme weather, and the internal phloem is responsible for transporting nutrients, and the bark of some trees can also store nutrients. However, biological invasions such as boring pests and pathogenic bacteria, natural factors such as extreme temperatures, lightning, strong winds, and fires, human activities such as mechanical collisions, improper pruning, and construction damage, and animal gnawing or rubbing can all damage the bark, expose the wood, form a wound, block the internal nutrient transport, and allow pathogenic bacteria to invade the tree, which poses a serious threat to the health and even survival of the tree. Tree wound repair is a common problem in garden maintenance and ancient tree protection, and existing methods for repairing a wound on a tree stem, such as applying a healing agent to the wound, skin grafting, bridging, biological repair methods, and physical protection methods, have certain effects, but also have many defects.

[0003] CN 109041971 A discloses a method for skin grafting a tree wound, including wound cleaning, wound disinfection, wound cutting, skin taking, medicine application, close adhesion, fixation, bandaging, and supervision, which can maximize the recovery of the tree wound through skin grafting to provide a nutrient transport channel required for the survival of the tree, but the skin grafting method relies on precise adhesion and has a strong dependence on donor trees, with a low success rate of grafting and a high risk of rejection; CN 108377829 A discloses a method for repairing a Cinnamomum camphora stem with a borer eye, including ring cutting of the stem, application of an indole acetic acid solution, wrapping of a moss matrix to make the ring cutting part root, and cutting and transplanting the stem below the rooting part when the root system is vigorous, which not only completely eradicates the borer damage, but also restores the ornamental value and timber value of the Cinnamomum camphora itself, but the repair period is long, the climate is highly limiting, the environmental adaptability is poor, and it is difficult to be widely used; CN 109197327 A discloses a method for bridging grafting an ancient tree, and CN 109832025 A discloses a method for rejuvenating an ancient tree by bridging grafting, both of which use a bridging grafting method to provide nutrients to the tree, but the bridging grafting requires a healthy mother tree for support, with a low and unstable survival rate; CN 102951959 A, CN 116616281 A, and CN 105967847 A all repair the stem wound by applying a healing agent, but the healing agents have complex compositions, poor adaptability to different tree species, and effects that are easily affected by temperature and humidity.

[0004] In addition, the existing trunk wound repair method also has the defect of small repair area. Therefore, it is urgent to develop a tree large-area wound repair technology without transplantation, simple operation, strong environmental adaptability, high survival rate and high universality to meet the needs of garden maintenance and ancient tree protection. SUMMARY

[0005] The present application aims to at least solve one of the above-mentioned technical problems in the prior art. To this end, the object of the present application is to provide a method for repairing a tree stem wound.

[0006] To achieve the above-mentioned object, the technical solution adopted by the present application is: The present application provides a method for repairing a tree stem wound, comprising the following steps: S1, root system induction stage: first clean, disinfect and preserve the wound, then cut the upper edge of the wound to expose the cambium, apply root system inducer to the incision, cover with peat soil substrate, seal, moisturize and shade; S2, root system traction stage: use a traction tube to guide the adventitious roots to the ground and manage water and fertilizer; S3, root system lignification promotion stage: promote root system lignification by light control, exogenous chemical regulation and periodic water control.

[0007] In some embodiments of the present application, the cleaning includes removing rotten bark and xylem.

[0008] In some embodiments of the present application, after cleaning, the operation of killing hidden insects using 7.0wt%-7.5wt% d-cycloprothrin insecticidal smoke is further included.

[0009] In some embodiments of the present application, the disinfection includes spraying the wound with 500-1000 times of carbendazim aqueous solution.

[0010] In some embodiments of the present application, the preservation treatment includes brushing benzene-free epoxy resin glue on the wound.

[0011] In some embodiments of the present application, the brushing frequency of the benzene-free epoxy resin glue is 2-4 times.

[0012] In some embodiments of the present application, the root system inducer includes indole acetic acid and kresoxim-methyl with clay slurry as the carrier; the content of the indole acetic acid and kresoxim-methyl in the clay slurry is respectively selected from 5-20mg / L.

[0013] In some embodiments of the present application, the clay slurry includes a mixture of clay and water with a mass ratio of (3-5): 1.

[0014] In some embodiments of the present application, the clay comprises at least one of yellow clay, red clay, and bentonite.

[0015] In some embodiments of the present application, the particle size of the clay is 60-80 mesh.

[0016] In some embodiments of the present application, the root system inducer is prepared by a method comprising the steps of: mixing clay with water to form a clay slurry; adding indole-3-acetic acid and fosetyl-Al into the clay slurry to obtain the root system inducer.

[0017] In some embodiments of the present application, the root system inducer is applied at a thickness of 2-3 mm at the beveled portion.

[0018] In some embodiments of the present application, the peat soil matrix comprises peat soil to which indole-3-acetic acid and fosetyl-Al are added; the amount of indole-3-acetic acid and fosetyl-Al added to the peat soil is selected from 5-20 mg / kg, respectively.

[0019] In some embodiments of the present application, the peat soil matrix is prepared by a method comprising the steps of: mixing indole-3-acetic acid and fosetyl-Al with peat soil, and stirring with water to obtain the peat soil matrix; wherein the amount of water added is determined by the criterion that the peat soil does not drip water when pinched tightly in the palm.

[0020] In some embodiments of the present application, during the root system induction stage, the peat soil matrix is pinched into an oblong cylindrical shape according to the transverse width of the wound, and then completely wrapped around the cut.

[0021] In some embodiments of the present application, the diameter of the oblong cylindrical peat soil matrix is 8-10 cm.

[0022] Specifically, the shape and size of the peat soil matrix can be adjusted adaptively according to the size and number of the wound on the tree trunk stem to ensure that the cut is fully covered and a stable moisture-retaining and root-promoting environment is provided.

[0023] In some embodiments of the present application, during the root system induction stage, after covering the peat soil matrix, the peat soil matrix is tightly wrapped with a film, and a light-shielding film is covered to seal and retain moisture and block light.

[0024] In some embodiments of the present application, during the curing period, the film is opened every 6-8 days to allow air to circulate, and the peat soil matrix is sprayed with atomized water to retain moisture, with the amount of water sprayed determined by the criterion that the peat soil matrix does not drip water.

[0025] In some embodiments of the present application, one end of the traction pipe is buried 10-20 cm underground, the other end is wrapped around the peat soil matrix at the cut, and the remaining part is arranged closely against the tree trunk and filled with peat soil matrix inside.

[0026] In some embodiments of the present application, the traction tube is a translucent plastic hose split in half.

[0027] In some embodiments of the present application, the diameter of the traction tube is 8-10 cm.

[0028] Specifically, the diameter of the traction tube can be selected according to the size and number of the wound surface of the tree trunk and the expected number of induced adventitious roots to ensure sufficient accommodation of the root system and provide good guidance.

[0029] In some embodiments of the present application, after the traction tube is arranged, it is fixed to the trunk and tightly wrapped with a film around the trunk, and the traction tube is covered with a light-shielding film for sealing, moisture retention and light-shielding maintenance.

[0030] In some embodiments of the present application, during the root traction stage, water and fertilizer management includes injecting 0.1wt%-0.5wt% phosphorus-potassium compound fertilizer into the peat soil substrate of the traction tube at a distance of 15-20 cm from the lowermost root; the injection frequency is 12-15 days / time.

[0031] In some embodiments of the present application, during the root lignification promotion stage, light control includes exposing the roots to weak light less than 1000 lux for 7-10 days, and then exposing the roots to natural light or artificial light of 2000-5000 lux.

[0032] In some preferred embodiments of the present application, during the root lignification promotion stage, light control specifically includes removing the light-shielding film above the traction tube to expose the roots to weak light less than 1000 lux, and then removing the traction tube and the film to expose the roots to full light of 2000-5000 lux after 7-10 days.

[0033] In some embodiments of the present application, during the root lignification promotion stage, exogenous chemical regulation includes: a) spraying 0.01wt%-0.1wt% high-potassium type foliar fertilizer on the leaves of the tree; b) spraying 0.01-0.1 μmol / L lignification promoter on the roots, with a spraying frequency of 6-8 days / time; c) spraying a mineral element solution containing 0.01wt%-0.05wt% Ca, 0.005wt%-0.01wt% B, and 0.001wt%-0.005wt% Cu on the roots, with a spraying frequency of 6-8 days / time.

[0034] In some embodiments of the present application, the high-potassium type foliar fertilizer includes 0.1wt%-0.3wt% monopotassium phosphate and 0.01wt%-0.03wt% potassium silicate.

[0035] In some preferred embodiments of the present application, the high-potassium type foliar fertilizer comprises 0.1wt%-0.2wt% monopotassium phosphate and 0.01wt%-0.02wt% potassium silicate.

[0036] In some embodiments of the present application, the high-potassium type foliar fertilizer is sprayed 1-2 times.

[0037] In some embodiments of the present application, the lignification promoter comprises epibrassidin (EBR).

[0038] In some embodiments of the present application, the mineral element solution comprises a mixed solution containing calcium chloride, boric acid and copper sulfate pentahydrate; wherein the content of calcium chloride is 0.028wt%-0.139wt%, the content of boric acid is 0.029wt%-0.057wt%, and the content of copper sulfate pentahydrate is 0.0039wt%-0.0197wt%.

[0039] In some embodiments of the present application, in the root system lignification promotion stage, the periodic water control comprises performing periodic drought-rehydration cycle on the peat soil substrate around the root system, and the single drought duration is 2-3 days.

[0040] In some embodiments of the present application, the duration of the root system lignification promotion stage is 4-8 weeks.

[0041] In some embodiments of the present application, the tree comprises at least one of Elaeocarpus glabripetalus, Ficus microcarpa, Ficus microcarpa, Plumeria alba and Cinnamomum camphora.

[0042] The basic principle of the present application is explained as follows: The tree stem wound repair method provided by the present application simulates the natural repair mechanism of the tree, greatly enhances and accelerates the process through artificial intervention, and finally realizes functional repair of large-area wounds by guiding the root system to replace the damaged bark. 1) Root system induction stage: first clean the wound and remove the rotten tissue and disinfect, clear the obstacles for new tissue, prevent bacteria competition and secondary infection, isolate the decay bacteria through antiseptic treatment, ensure the new root system to start in a clean environment; then oblique cut on the healthy part of the wound edge, accurately expose the cambium cells with the strongest meristem ability as the source of adventitious root formation, and smear root system inducer on the incision, indole acetic acid in the root system inducer is the core hormone signal stimulating cell division and root primordium formation, and the soil bacteria can protect the fragile incision from soil-borne bacteria, and the clay slurry is used as a carrier, which is convenient and low in cost, and can enhance the adhesion of functional components; peat soil matrix covering can provide dark, moisture and ventilation physical environment, which simulates the ideal conditions for root growth in soil; darkness is the necessary condition for the initiation and growth of root primordium, so the maintenance method of sealing, moisturizing and shading is adopted, and the lightproof property of the internal environment is strictly ensured; 2) Root system traction stage: this stage mainly uses the geotropism and haptotropism of roots, builds a protected artificial soil environment from the new root system to the ground through the traction pipe, which can guide the geotropic growth of root system on one hand, and prevent the root system from drying or aging in the air on the other hand; fill the new substrate in the pipe to provide a continuous and familiar growth medium for the forward extension of the root system; periodically inject phosphorus and potassium compound fertilizer into the lower end of the traction pipe, use the haptotropism of roots to strengthen the tendency of geotropic growth, provide bait and energy for root growth, and ensure that it can successfully grasp the ground; 3) Root system lignification stage: this stage sprays high potassium type foliar fertilizer (such as phosphorus and potassium fertilizer, potassium silicate) on the leaf surface, absorbs and synthesizes nutrients through the leaf, and transports downward through the remaining vascular system to provide logistical support for the maturation of the root system, and silicon elements can also enhance the strength of the cell wall; spray brassinolide and mineral element solution on the root system, brassinolide can significantly promote the differentiation of plant cells and the synthesis of lignin, which is a key hormone signal to accelerate the development of root vascular bundle and lignification, in the mineral element solution containing Ca, B and Cu, calcium is a component of the gel layer in the cell wall, boron participates in the formation and transport of lignin, and copper is a cofactor of lignin synthesis enzyme, so the mineral element solution directly provides the key raw materials for building cell wall and lignin; through progressive lightening, the seedling is simulated to break the soil, and the root system is gradually adapted to the light environment to avoid stress reaction; through the drought-rehydration cycle, moderate water stress can stimulate plants to produce abscisic acid, thereby inducing the root system to be more developed, and accelerating the differentiation and thickening of the xylem vessels, enhancing its stress resistance and water transport efficiency; this stage promotes the development of root vascular tissue and the lignification and reinforcement of cell wall based on nutrition and hormone regulation, and environmental stress training, so that the root system has the functions of transportation and support similar to the xylem of the trunk.

[0043] Compared with the prior art, the beneficial effects of the present application are: 1) The method for repairing tree trunk wounds provided by this invention induces the rapid generation of adventitious roots from the bark of the tree trunk wound. The roots are then introduced into the ground using traction tubes and water and fertilizer induction. Then, through measures such as light control, exogenous chemical regulation, and periodic water control, the nutrient transport function of the bark is restored, allowing the tree to resume normal growth. This method does not require bark transplantation, does not rely on donor trees, and has a high survival rate. It does not rely on specific healing agents, and the effect is less affected by temperature and humidity. It is widely applicable to various tree species. It does not require trunk cutting or transplantation, is simple to operate, and has strong environmental adaptability. 2) The tree trunk wound repair method provided by the present invention can repair large-area wounds, efficiently restore the tree's nutrient transport function, and realize the tree's in-situ rejuvenation and healthy growth. Attached Figure Description

[0044] Figure 1 The image shown is a photograph of the stem wound of *Elaeocarpus acuminata* in the example. Figure 2 The image shown is a photograph of the wound cleaning process of *Elaeocarpus acuminata* in the example. Figure 3 This is a photograph of the root system after adventitious roots were induced in *Elaeocarpus acuminata* in the example. Figure 4 The image shown is a photograph of the root system of *Elaeocarpus acuminata* being pulled to the ground in the example. Figure 5 The image shown is a photograph of the successful root system of *Elaeocarpus acuminata* in the example, which was then successfully guided to the ground and lignified. Detailed Implementation

[0045] The present invention will be further described in detail below through specific embodiments. Unless otherwise specified, the raw materials, reagents, or apparatus used in the embodiments can be obtained from conventional commercial sources or by existing technical methods. Unless otherwise specified, the experimental or testing methods are conventional methods in the art.

[0046] Example This embodiment provides a method for repairing wounds on tree trunks, and uses this method to repair a damaged *Elaeocarpus lanceolata* tree. The *Elaeocarpus lanceolata* has a diameter at breast height (DBH) of approximately 35 cm, with a large area of ​​long, strip-shaped damage to the bark of the trunk, approximately 0.8 m in length, and an average width of about 1 / 3 of the cross-sectional perimeter (the maximum width is about 2 / 3 of the cross-sectional perimeter). Some branches and leaves are withered and yellow, and the tree is in critical condition. The repair steps are as follows: S11. Cleaning the stem wound: Use a scraper and grinder to clean the rotten bark and wood of the wound. Use 7.2wt% dextrorotatory cypermethrin insecticide fogging agent to kill hidden insects. Then spray the wound with 800 times carbendazim aqueous solution for disinfection. After disinfection and the wound is completely dry, waterproof and anti-corrosion treatment is applied to the exposed wood. The specific method is to brush a layer of benzene-free epoxy resin on the surface of the wood, and brush another layer after it dries, until three layers of benzene-free epoxy resin are applied. S12, pass the yellow mud through a 60-80 mesh stainless steel sieve, mix the undersize yellow mud with water at a mass ratio of 4:1 to form a yellow mud slurry, then add indole acetic acid and boscalid to the yellow mud slurry, both at a content of 10 mg / L in the yellow mud slurry, mix thoroughly to obtain a root system inducer; make an oblique cut on the upper region of the wound with a knife, exposing the cambium layer, and apply the root system inducer to the cut, with an application thickness of 2-3 mm; S13, mix indole acetic acid and boscalid with peat, with an addition amount of indole acetic acid and boscalid in the peat of 10 mg / kg, add water and stir, with the amount of water added being such that the peat does not drip when pinched tightly in the palm, mix thoroughly to obtain a peat substrate; knead the peat substrate to form a long cylindrical shape with a diameter of 10 cm, completely wrap the cut, then tightly wrap the tree trunk with plastic wrap, and then wrap a layer of black light-shielding film to make the inside not transparent to light; S14, every other week, remove the plastic wrap and light-shielding film to allow air to pass through, if the substrate is relatively dry, spray atomized water, with the amount of water sprayed being such that the substrate does not drip, and maintain until adventitious roots are formed; S21, remove the plastic wrap and light-shielding film to expose the peat substrate, use a translucent plastic hose with a pore size of 10 cm and cut in half to make a root system traction pipe, arrange the root system traction pipe from bottom to top, with one end inserted into the ground to a depth of 15 cm, and the other end wrapped around the peat substrate at the cut, the rest of the traction pipe is arranged tightly against the tree trunk, and is filled with new peat substrate inside; after the traction pipe is arranged, it is fixed to the stem with a tie, then the traction pipe and the tree trunk are wrapped with plastic wrap, and finally wrapped with a black light-shielding film to ensure internal insulation, moisture retention and light shielding; S22, observe the root system growth regularly, every 14 days, inject a phosphorus-potassium compound fertilizer with a concentration of 0.3 wt% into the peat substrate in the traction pipe 15-20 cm away from the lowermost root system, to induce the newly formed root system to grow towards the ground, until sufficient root systems fall to the ground; S31, spraying 0.1wt% potassium dihydrogen phosphate and 0.01wt% potassium silicate on the leaves of the tree to promote root maturation; removing the black light-shielding film so that part of the light can pass through the plastic wrap and the translucent traction tube, for 7-10 days, then removing the plastic wrap and the translucent traction tube to expose the roots to full light, with an intensity of 2000-5000 lux; during this period, spraying the roots with 0.05μmol / L epibrassinolide every 7 days to promote xylem cell differentiation and enhance cell wall cross-linking, while spraying the roots with a mixture containing 0.1wt% calcium chloride, 0.05wt% boric acid and 0.01wt% copper sulfate every 7 days to provide calcium, boron and copper elements that are essential for lignin cross-linking; spraying the peat soil substrate with water again after it has been dried for 2-3 days to control the water content of the roots through periodic drought-rehydration cycles; when the roots change from milky white / translucent to light brown to reddish brown, it means that the roots have successfully lignified and can provide nutrients to the tree normally, and the development of thick and strong roots can also provide strong support to the tree.

[0047] Figure 1 Figure for the wound surface of the stem of Elaeocarpus glabripetalis in the example, Figure 2 Figure for the wound surface cleaning of Elaeocarpus glabripetalis in the example, Figure 3 Figure for the root traction of Elaeocarpus glabripetalis after inducing adventitious roots in the example, wherein, Figure 3 (a) and (b) in Figure are photographs taken from different angles, Figure 4 Figure for the root traction landing of Elaeocarpus glabripetalis in the example, Figure 5 Figure for the successful root traction landing and lignification of Elaeocarpus glabripetalis in the example, from Figures 1-5 It can be seen that the damaged area of the stem of Elaeocarpus glabripetalis is large, and after using the repair method provided by the application, adventitious roots are formed at the wound incision on the 26th day of the root induction stage, and the adventitious roots successfully land after half a year; during the root lignification promotion stage, the roots recover the nutrient transport function of the bark after 5 weeks of lignification conversion, and the growth of the Elaeocarpus glabripetalis tree also returns to normal.

Claims

1. A method for repairing wounds on tree trunks, characterized in that, Includes the following steps: S1. Root induction stage: First, clean, disinfect and prevent decay of the wound. Then, make an oblique cut at the upper edge of the wound to expose the cambium. Apply root induction agent to the cut and cover with peat moss substrate. Seal and keep moist and shade it for maintenance. S2, Root Traction Stage: Using traction pipes to guide adventitious roots to the ground and manage water and fertilizer; S3, Root Lignification Stage: Root lignification is promoted through light control, exogenous chemical regulation, and periodic water control.

2. The repair method according to claim 1, characterized in that, The root inducer includes indoleacetic acid and soil fungicide carried on clay slurry; the content of indoleacetic acid and soil fungicide in the clay slurry is selected from 5-20 mg / L, respectively.

3. The repair method according to claim 2, characterized in that, The root inducing agent is applied to the obliquely cut area with a thickness of 2-3 mm.

4. The repair method according to claim 1, characterized in that, The peat substrate includes peat with added indoleacetic acid and soil fungicide; the amount of indoleacetic acid and soil fungicide added to the peat is selected from 5-20 mg / kg, respectively.

5. The repair method according to claim 1, characterized in that, One end of the traction tube is buried 10-20cm underground, the other end is wrapped with peat moss matrix at the cut, and the rest is arranged close to the tree trunk and filled with peat moss matrix inside.

6. The repair method according to claim 5, characterized in that, During the root traction stage, water and fertilizer management includes injecting 0.1wt%-0.5wt% of phosphorus and potassium compound fertilizer into the peat substrate of the traction tube 15-20cm away from the lowest root; the injection frequency is 12-15 days / time.

7. The repair method according to claim 1, characterized in that, During the root lignification stage, light control includes exposing the roots to weak light (less than 1000 lux) for 7-10 days, followed by exposure to natural or artificial light (2000-5000 lux).

8. The repair method according to claim 1, characterized in that, During the root lignification stage, exogenous chemical regulation includes: a) Spray the tree leaves with 0.01wt%-0.1wt% high-potassium foliar fertilizer; b) Spray the roots with 0.01-0.1 μmol / L lignification accelerator every 6-8 days. c) Spray the roots with a mineral element solution containing 0.01wt%-0.05wt% Ca, 0.005wt%-0.01wt% B, and 0.001wt%-0.005wt% Cu, at a frequency of 6-8 days / time.

9. The repair method according to claim 1, characterized in that, During the root lignification stage, periodic water control includes periodic drought-rehydration cycles on the peat substrate surrounding the roots, with each drought lasting 2-3 days.

10. The repair method according to any one of claims 1-9, characterized in that, The trees include at least one of Elaeocarpus acuminata, Ficus microcarpa, Vitex negundo, Plumeria rubra, and Cinnamomum camphora.

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