Broad-spectrum plant-derived disease-resistant excitant for rice blast and preparation method thereof
By combining multiple plant-derived components and using precise preparation processes, a broad-spectrum plant-derived disease resistance activator was prepared, solving the problems of single component and uneven preparation of traditional activators, and achieving effective control of rice blast and promotion of plant growth.
Patent Information
- Application Number
- CN202511277902.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-09-09
- Publication Date
- 2026-01-02
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
Traditional plant-derived disease resistance activators have single components and limited disease resistance effects. Furthermore, the preparation process suffers from uneven composition and poor solubility, which affects the application effect and stability.
A broad-spectrum plant-derived disease-resistant activator was prepared by combining potassium glutamate, S-allyl-L-cysteine, 2-piperidinic acid, chitosan, salicylic acid, vitamin E, plant extracts, and essential oils, through precise weighing, stirring, and filtration. The pH value was adjusted and stabilized to ensure the homogeneity and clarity of the solution.
It enhances the plant's disease resistance, promotes growth, effectively inhibits the growth and reproduction of rice blast fungus, improves the plant's defense response, and provides comprehensive protection.
Smart Images
Figure CN121242032A_ABST
Abstract
Description
Technical Field
[0001] This invention belongs to the field of rice blast prevention and control technology, specifically a broad-spectrum plant-derived disease resistance activator against rice blast and its preparation method. Background Technology
[0002] Rice blast is one of the major diseases in rice production, seriously affecting rice yield and quality. Traditionally, the control of rice blast has mainly relied on chemical pesticides, such as copper-containing fungicides. However, long-term use of chemical pesticides can lead to problems such as pathogen resistance, environmental pollution, and pesticide residues in agricultural products, posing a threat to human health and the ecological environment. Therefore, broad-spectrum plant-derived disease resistance activators can be used.
[0003] However, traditional plant-derived disease resistance activators often have a single component, limited disease resistance effect, and little effect on promoting plant growth. In addition, existing activators may have problems such as uneven composition and poor solubility during preparation, which affect their application effect and stability. Summary of the Invention
[0004] The purpose of this invention is to provide a broad-spectrum plant-derived disease resistance activator against rice blast and its preparation method in order to solve the problems mentioned above.
[0005] The technical solution adopted in this invention is as follows: a broad-spectrum plant-derived disease resistance activator against rice blast, comprising: potassium glutamate: 15-25 parts by weight; S-allyl-L-cysteine: 10-20 parts by weight; 2-piperidinic acid: 5-15 parts by weight; chitosan: 3-7 parts by weight; salicylic acid: 1-3 parts by weight; vitamin E: 0.5-1.5 parts by weight; polyglutamic acid: 2-4 parts by weight; dandelion extract: 3-7 parts by weight; baicalin: 2-4 parts by weight; lemongrass oil: 1-3 parts by weight; tea tree oil: 1-3 parts by weight. Parts by weight; andrographolide: 1-3 parts by weight; houttuynin: 1-3 parts by weight; chlorogenic acid: 1-3 parts by weight; licorice extract: 1-3 parts by weight; aloe extract: 1-3 parts by weight; peppermint oil: 0.5-1.5 parts by weight; basil oil: 0.5-1.5 parts by weight; rosemary extract: 0.5-1.5 parts by weight; thymol: 0.5-1.5 parts by weight; eucalyptus oil: 0.5-1.5 parts by weight; cinnamaldehyde: 0.3-0.7 parts by weight and anethole: 0.3-0.7 parts by weight.
[0006] In a preferred embodiment, a method for preparing a broad-spectrum plant-derived disease resistance activator against rice blast includes the following steps:
[0007] S1: Prepare the raw materials. Weigh each ingredient according to the formula requirements to ensure that the weight of each ingredient is within the specified range.
[0008] S2: Dissolve the solid components such as potassium glutamate, S-allyl-L-cysteine, 2-piperidinic acid, chitosan, and salicylic acid in an appropriate amount of water to form a homogeneous solution.
[0009] S3: Mix the dissolved solid components together and stir evenly to ensure the homogeneity of the solution;
[0010] S4: Add vitamin E and polyglutamic acid to the mixed solution and continue stirring until completely dissolved;
[0011] S5: Gradually add the plant extracts and essential oils such as dandelion extract, baicalin, lemongrass oil, tea tree oil, andrographolide, houttuynin, chlorogenic acid, licorice extract, aloe vera extract, peppermint oil, basil oil, rosemary extract, thymol, eucalyptol, cinnamaldehyde, and anethole to the mixed solution while stirring to ensure thorough mixing;
[0012] S6: Adjust the pH of the solution with an appropriate amount of acid or base to achieve a suitable pH range, usually neutral or slightly acidic;
[0013] S7: Stabilize the mixed solution to ensure the stability and effectiveness of the product;
[0014] S8: Filter the mixed solution through a filter to remove any insoluble matter or impurities and ensure the clarity of the solution;
[0015] S9: Package the filtered clarified solution and store it in a cool, dry, and ventilated place, avoiding direct sunlight and high temperatures, in order to maintain the quality and effectiveness of the product.
[0016] In a preferred embodiment, in step S1, a high-precision electronic balance is used for weighing to ensure that the weight of each component is within a specified range, such as 15-25 parts of potassium glutamate and 10-20 parts of S-allyl-L-cysteine. During weighing, care should be taken to avoid cross-contamination, and each component should be weighed using a separate weighing sheet or container. All raw materials should be stored in a dry, clean environment to avoid moisture absorption or contamination.
[0017] In a preferred embodiment, during step S2, the water temperature is controlled at 40-50°C to accelerate the dissolution process. After each component is dissolved, stirring should be used to ensure the solution is homogeneous and free of undissolved particles. The dissolved solution should be clear and free of sediment; if necessary, preliminary filtration can be performed using filter paper.
[0018] In a preferred embodiment, in step S3, a magnetic stirrer or mechanical stirrer is used for stirring at a speed of 200-300 rpm for at least 30 minutes to ensure that all components are fully mixed and the solution reaches a homogeneous state. During the mixing process, the color and transparency of the solution should be carefully observed to ensure that there is no layering or precipitation.
[0019] In step S4, vitamin E is pre-dissolved in a small amount of ethanol to promote its dispersion in water. Polyglutamic acid is added gradually to avoid excessively high local concentrations caused by adding it all at once. The stirring speed is maintained at 200-300 rpm for at least 15 minutes until completely dissolved and the solution is a uniform milky white.
[0020] In a preferred embodiment, in step S5, the essential oil components can be pre-emulsified with an emulsifier such as Tween-80 to improve their dispersibility in water. During addition, the components should be added slowly dropwise while continuously stirring at a speed of 300-400 rpm for at least 30 minutes to ensure thorough mixing of all components and no obvious stratification of the solution.
[0021] In a preferred embodiment, in step S6, dilute hydrochloric acid or sodium hydroxide solution is used to adjust the pH value to a suitable range, typically 6.0-7.0. During adjustment, the acid or alkali solution should be added slowly while stirring to avoid over-addition. The adjusted solution should be stirred again to ensure a uniform pH distribution.
[0022] In a preferred embodiment, in step S7, the solution is heated to 60-70°C and held for 15-20 minutes, then slowly cooled to room temperature. Stabilizers, including xanthan gum or sodium carboxymethyl cellulose, are added to enhance the stability and suspension properties of the solution. After stabilization, the solution should show no significant precipitation or stratification.
[0023] In a preferred embodiment, in step S8, the mixed solution is filtered through a 0.22 μm microporous membrane to remove any insoluble matter or impurities. During filtration, reduced pressure filtration or a peristaltic pump should be used to assist filtration to ensure effectiveness. The filtered solution should be clear and transparent, free of suspended matter or sediment. If necessary, secondary filtration can be performed until satisfactory clarity is achieved.
[0024] In a preferred embodiment, in step S9, the product is stored in a cool, dry, and well-ventilated place, with the storage temperature controlled between 10-25°C, avoiding direct sunlight and high temperatures. The product should be labeled with the production date, batch number, and instructions for use to ensure correct use by the user.
[0025] In summary, due to the adoption of the above technical solution, the beneficial effects of the present invention are:
[0026] 1. In this invention, potassium glutamate, as an amino acid salt, not only provides nitrogen to plants but also participates in metabolic processes within the plant, enhancing its resistance to adverse conditions. S-allyl-L-cysteine, a sulfur-containing amino acid derivative, possesses antioxidant and disease-resistant activities, stimulating plant defense mechanisms to combat rice blast fungus infection. 2-Piperidinic acid inhibits pathogen growth and directly interferes with cell wall synthesis in rice blast fungus, thereby mitigating disease. Chitosan, as a biopolysaccharide, can induce plants to produce resistance substances, strengthen plant cell walls, form a physical barrier, and prevent pathogen invasion. Salicylic acid is an important signaling molecule in plants, capable of activating systemic acquired resistance (SAR) and improving overall disease resistance.
[0027] 2. In this invention, Vitamin E protects plant cells from oxidative damage and maintains cell membrane integrity, thereby enhancing the plant's resistance to rice blast. Polyglutamic acid has excellent moisturizing and nourishing effects, improving the plant's growth environment, promoting healthy plant growth, and indirectly enhancing disease resistance. Plant extracts such as dandelion extract, baicalin, andrographolide, and houttuynin contain abundant bioactive components, such as flavonoids, saponins, and alkaloids. These components possess various biological activities, including antibacterial, anti-inflammatory, and immunomodulatory effects, effectively inhibiting the growth and reproduction of rice blast fungus while stimulating the plant's internal defense system. Chlorogenic acid, licorice extract, and aloe vera extract also possess similar biological activities, enhancing the plant's disease resistance and self-repair capabilities.
[0028] 3. In this invention, the essential oil components such as lemongrass oil, tea tree oil, peppermint oil, basil oil, rosemary extract, thymol, eucalyptus oil, cinnamaldehyde, and anethole are volatile and can inhibit the spore germination and mycelial growth of rice blast fungus through gas-phase interactions. These essential oils contain abundant terpenes, aldehydes, phenols, and other compounds, exhibiting strong antibacterial and antifungal activities, capable of directly killing or inhibiting pathogens. Simultaneously, the essential oil components can enter the plant through its stomata, stimulating the plant's defense response and enhancing its overall disease resistance. Furthermore, the essential oil components offer additional benefits such as pest repellency and soil environment improvement, providing more comprehensive protection for rice. In summary, the various components of this formula work together through multiple mechanisms to form an effective broad-spectrum plant-derived disease resistance stimulant, contributing to the prevention and control of rice blast disease occurrence and spread. Attached Figure Description
[0029] Figure 1 This is a schematic diagram illustrating the process principle of the present invention. Detailed Implementation
[0030] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the invention.
[0031] Reference Figure 1 ,
[0032] Example:
[0033] A broad-spectrum plant-derived disease resistance activator against rice blast includes: 20 parts by weight of potassium glutamate, 15 parts by weight of S-allyl-L-cysteine, 10 parts by weight of 2-piperidinic acid, 5 parts by weight of chitosan, 2 parts by weight of salicylic acid, 1 part by weight of vitamin E, 3 parts by weight of polyglutamic acid, 5 parts by weight of dandelion extract, 3 parts by weight of baicalin, 2 parts by weight of lemongrass oil, 2 parts by weight of tea tree oil, 2 parts by weight of andrographolide, 2 parts by weight of houttuynin, 2 parts by weight of chlorogenic acid, 2 parts by weight of licorice extract, 2 parts by weight of aloe extract, 1 part by weight of peppermint oil, 1 part by weight of basil oil, 1 part by weight of rosemary extract, 1 part by weight of thymol, 1 part by weight of eucalyptus oil, 0.5 parts by weight of cinnamaldehyde, and 0.5 parts by weight of anethole.
[0034] The method includes the following steps:
[0035] S1: Prepare the raw materials. Weigh each ingredient according to the formula requirements, ensuring that the weight of each ingredient is within the specified range.
[0036] S2: Dissolve the solid components, such as potassium glutamate, S-allyl-L-cysteine, 2-piperidinic acid, chitosan, and salicylic acid, in an appropriate amount of water to form a homogeneous solution.
[0037] S3: Mix the dissolved solid components together and stir thoroughly to ensure the homogeneity of the solution.
[0038] S4: Add vitamin E and polyglutamic acid to the mixed solution and continue stirring until completely dissolved.
[0039] S5: Gradually add the following plant extracts and essential oils to the mixed solution: dandelion extract, baicalin, lemongrass oil, tea tree oil, andrographolide, houttuynin, chlorogenic acid, licorice extract, aloe vera extract, peppermint oil, basil oil, rosemary extract, thymol, eucalyptol, cinnamaldehyde, and anethole, while stirring constantly to ensure thorough mixing.
[0040] S6: Adjust the pH of the solution using an appropriate amount of acid or alkali to achieve a suitable pH range, typically neutral or slightly acidic.
[0041] S7: Stabilize the mixed solution to ensure the stability and effectiveness of the product.
[0042] S8: Filter the mixed solution through a filter to remove any insoluble matter or impurities, ensuring the clarity of the solution.
[0043] S9: Package the filtered clarified solution and store it in a cool, dry, and ventilated place, avoiding direct sunlight and high temperatures, in order to maintain the quality and effectiveness of the product.
[0044] In step S1, a high-precision electronic balance is used for weighing to ensure that the weight of each component is within the specified range, such as 15-25 parts of potassium glutamate and 10-20 parts of S-allyl-L-cysteine. Care should be taken to avoid cross-contamination during weighing; each component should be weighed using a separate weighing sheet or container. All raw materials should be stored in a dry, clean environment to prevent moisture absorption or contamination.
[0045] In step S2, the water temperature is controlled at 40-50℃ during the dissolution process to accelerate it. After each component dissolves, stirring should be used to ensure the solution is homogeneous and free of undissolved particles. The dissolved solution should be clear and free of precipitate; if necessary, preliminary filtration can be performed using filter paper.
[0046] In step S3, use a magnetic stirrer or mechanical stirrer to stir at a speed of 200-300 rpm for at least 30 minutes to ensure all components are thoroughly mixed and the solution becomes homogeneous. During mixing, carefully observe the color and transparency of the solution to ensure there is no layering or precipitation.
[0047] In step S4, vitamin E is pre-dissolved in a small amount of ethanol to promote its dispersion in water. Polyglutamic acid is added gradually to avoid excessively high local concentrations caused by adding it all at once. The stirring speed is maintained at 200-300 rpm for at least 15 minutes until completely dissolved and the solution is a uniform milky white.
[0048] In step S5, essential oil components can be pre-emulsified with an emulsifier such as Tween-80 to improve their dispersibility in water. During addition, the components should be added slowly dropwise while continuously stirring at a speed of 300-400 rpm for at least 30 minutes to ensure thorough mixing of all components and no obvious layering of the solution.
[0049] In step S6, dilute hydrochloric acid or sodium hydroxide solution is used to adjust the pH value to a suitable range, typically 6.0-7.0. During adjustment, the acid or alkali solution should be added slowly while stirring continuously to avoid over-addition. The adjusted solution should be stirred again to ensure a uniform pH distribution.
[0050] In step S7, heat to 60-70°C and maintain for 15-20 minutes, then slowly cool to room temperature. Add stabilizers, including xanthan gum or sodium carboxymethyl cellulose, to enhance the stability and suspension properties of the solution. After stabilization, the solution should show no obvious precipitation or stratification.
[0051] In step S8, the mixed solution is filtered through a 0.22 μm microporous membrane to remove any insoluble matter or impurities. During filtration, reduced pressure filtration or a peristaltic pump should be used to assist filtration to ensure effectiveness. The filtered solution should be clear and transparent, free of suspended matter or sediment. If necessary, secondary filtration may be performed until satisfactory clarity is achieved.
[0052] In step S9, store the product in a cool, dry, and well-ventilated place, maintaining a storage temperature of 10-25℃, and avoid direct sunlight and high temperatures. The product should be labeled with the production date, batch number, and instructions for use to ensure correct usage by the user.
[0053] Experimental Example: Evaluation of the control effect of broad-spectrum plant-derived disease resistance activators on rice blast.
[0054] I. Experimental Objective
[0055] To verify the efficacy of the above-mentioned broad-spectrum plant-derived disease resistance activator against rice blast, field trials and data analysis were conducted to evaluate its impact on rice growth and disease resistance.
[0056] II. Test Materials
[0057] Rice varieties: Select local rice varieties that are susceptible to rice blast.
[0058] Broad-spectrum plant-derived disease resistance activator: Prepared according to the above formula.
[0059] Control group: conventional pesticides (such as copper-containing fungicides).
[0060] Experimental fields: Select experimental fields with consistent soil conditions and management levels, and divide them into treatment groups and control groups.
[0061] III. Test Methods
[0062] Experimental design: A randomized block design was adopted, with 3 replicates for each treatment and a plot area of 30 square meters for each plot.
[0063] Application method: Apply the pesticide once each during the tillering stage and the booting stage of rice. Dilute the activator 1000 times and spray. The control group is treated with the conventional dosage.
[0064] Disease survey: After application of pesticides, the incidence of rice blast should be investigated regularly, and the disease leaf rate and disease index should be recorded.
[0065] Growth monitoring: Monitor the growth of rice, including plant height and biomass.
[0066] Data analysis: SPSS software was used for data analysis to compare the differences between the treatment group and the control group.
[0067] IV. The data table is shown below:
[0068] in:
[0069] Disease index = ∑(number of diseased leaves at each level × relative value) / (total number of leaves surveyed × 9) × 100
[0070] Control efficacy (%) = [1 - (Disease index before application in control area × Disease index after application in treatment area) / (Disease index after application in control area × Disease index before application in treatment area)] × 100
[0071]
[0072] V. Results Analysis
[0073] Disease incidence and disease index: The disease incidence and disease index of the activator treatment group were significantly lower than those of the control group, indicating that the activator effectively reduced the severity of rice blast.
[0074] Plant height and biomass: The plant height and biomass of the activator treatment group were significantly higher than those of the control group, indicating that the activator not only has the effect of preventing and controlling diseases, but also promotes rice growth.
[0075] From the above, we can conclude that:
[0076] In this invention, potassium glutamate, as an amino acid salt, not only provides nitrogen to plants but also participates in metabolic processes within the plant, enhancing its stress resistance. S-allyl-L-cysteine, a sulfur-containing amino acid derivative, possesses antioxidant and disease-resistant activities, stimulating plant defense mechanisms to combat rice blast fungus infection. 2-Piperidinic acid inhibits pathogen growth, directly interfering with cell wall synthesis in rice blast fungus, thereby mitigating disease. Chitosan, as a biopolysaccharide, can induce plants to produce resistance substances, strengthen plant cell walls, form a physical barrier, and prevent pathogen invasion. Salicylic acid, an important signaling molecule in plants, can activate systemic acquired resistance (SAR) and improve overall disease resistance.
[0077] In this invention, Vitamin E is a potent antioxidant that protects plant cells from oxidative damage and maintains cell membrane integrity, thereby enhancing the plant's resistance to rice blast. Polyglutamic acid has excellent moisturizing and nourishing effects, improving the plant's growing environment, promoting healthy growth, and indirectly enhancing disease resistance. Plant extracts such as dandelion extract, baicalin, andrographolide, and houttuynin contain abundant bioactive components, including flavonoids, saponins, and alkaloids. These components possess various biological activities such as antibacterial, anti-inflammatory, and immunomodulatory effects, effectively inhibiting the growth and reproduction of rice blast fungus while stimulating the plant's internal defense system. Chlorogenic acid, licorice extract, and aloe vera extract also exhibit similar biological activities, enhancing the plant's disease resistance and self-repair capabilities.
[0078] In this invention, essential oil components such as lemongrass oil, tea tree oil, peppermint oil, basil oil, rosemary extract, thymol, eucalyptus oil, cinnamaldehyde, and anethole are volatile and can inhibit spore germination and mycelial growth of rice blast fungus through gas-phase interactions. These essential oils are rich in terpenes, aldehydes, phenols, and other compounds, exhibiting strong antibacterial and antifungal activities, capable of directly killing or inhibiting pathogens. Simultaneously, the essential oil components can enter the plant through stomata, stimulating the plant's defense response and enhancing its overall disease resistance. Furthermore, the essential oil components offer additional benefits such as pest repellency and soil environment improvement, providing more comprehensive protection for rice. In summary, the various components of this formulation work together through multiple mechanisms to form an effective broad-spectrum plant-derived disease resistance stimulant, contributing to the prevention and control of rice blast disease occurrence and spread.
[0079] It should be noted that, in this document, relational terms such as "first" and "second" are used merely to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the term "comprising" or any other variations thereof is intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitations, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes said element.
[0080] The foregoing description enables those skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the invention. Therefore, the invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims
1. A broad-spectrum plant-derived disease resistance activator against rice blast, characterized in that: Includes: potassium glutamate: 15-25 parts by weight; S-allyl-L-cysteine: 10-20 parts by weight; 2-Piperidinic acid: 5-15 parts by weight; Chitosan: 3-7 parts by weight; Salicylic acid: 1-3 parts by weight; Vitamin E: 0.5-1.5 parts by weight; Polyglutamic acid: 2-4 parts by weight; Dandelion extract: 3-7 parts by weight; Baicalin: 2-4 parts by weight; Lemongrass oil: 1-3 parts by weight; Tea tree oil: 1-3 parts by weight; Andrographolide: 1-3 parts by weight; Houttuynin: 1-3 parts by weight; Chlorogenic acid: 1-3 parts by weight; Licorice extract: 1-3 parts by weight; Aloe extract: 1-3 parts by weight; Peppermint oil: 0.5-1.5 parts by weight; Basil oil: 0.5-1.5 parts by weight; Rosemary extract: 0.5-1.5 parts by weight; Thymol: 0.5-1.5 parts by weight; Eucalyptus oil: 0.5-1.5 parts by weight; Cinnamaldehyde: 0.3-0.7 parts by weight; and Anethole: 0.3-0.7 parts by weight.
2. The method for preparing the broad-spectrum plant-derived disease resistance activator against rice blast as described in claim 1, characterized in that: The method includes the following steps: S1: Prepare the raw materials. Weigh each ingredient according to the formula requirements to ensure that the weight of each ingredient is within the specified range. S2: Dissolve potassium glutamate, S-allyl-L-cysteine, 2-piperidinic acid, chitosan, and salicylic acid solid components in an appropriate amount of water to form a homogeneous solution. S3: Mix the dissolved solid components together and stir evenly to ensure the homogeneity of the solution; S4: Add vitamin E and polyglutamic acid to the mixed solution and continue stirring until completely dissolved; S5: Gradually add dandelion extract, baicalin, lemongrass oil, tea tree oil, andrographolide, houttuynin, chlorogenic acid, licorice extract, aloe vera extract, peppermint oil, basil oil, rosemary extract, thymol, eucalyptus oil, cinnamaldehyde, and anethole plant extracts and essential oils to the mixed solution while stirring to ensure thorough mixing; S6: Adjust the pH of the solution with an appropriate amount of acid or base to achieve a suitable pH range, usually neutral or slightly acidic; S7: Stabilize the mixed solution to ensure the stability and effectiveness of the product; S8: Filter the mixed solution through a filter to remove any insoluble matter or impurities and ensure the clarity of the solution; S9: Package the filtered clarified solution and store it in a cool, dry, and ventilated place, avoiding direct sunlight and high temperatures, in order to maintain the quality and effectiveness of the product.
3. The method for preparing the broad-spectrum plant-derived disease resistance activator against rice blast as described in claim 1, characterized in that: In step S1, a high-precision electronic balance is used for weighing to ensure that the weight of each component is within the specified range: 15-25 parts of potassium glutamate and 10-20 parts of S-allyl-L-cysteine. When weighing, care should be taken to avoid cross-contamination, and each component should be weighed using an independent weighing paper or container. All raw materials should be stored in a dry and clean environment to avoid moisture absorption or contamination.
4. The method for preparing the broad-spectrum plant-derived disease resistance activator against rice blast as described in claim 1, characterized in that: In step S2, the water temperature is controlled at 40-50℃ during the dissolution process to accelerate the dissolution process; after each component is dissolved, the solution is stirred to ensure that it is uniform and free of undissolved particles.
5. The method for preparing a broad-spectrum plant-derived disease resistance activator against rice blast as described in claim 1, characterized in that: In step S3, a magnetic stirrer or mechanical stirrer is used for stirring. The stirring speed is controlled at 200-300 rpm, and the stirring time is not less than 30 minutes to ensure that all components are fully mixed and the solution reaches a homogeneous state. During the mixing process, pay attention to the color and transparency of the solution to ensure that there is no layering or precipitation. In step S4, vitamin E is dissolved in a small amount of ethanol beforehand to promote its dispersion in water; polyglutamic acid is added gradually to avoid excessively high local concentrations caused by adding it all at once; the stirring speed is maintained at 200-300 rpm, and the stirring time is not less than 15 minutes until it is completely dissolved and the solution is uniformly milky white.
6. The method for preparing the broad-spectrum plant-derived disease resistance activator against rice blast as described in claim 1, characterized in that: In step S5, the essential oil components can be pre-emulsified with Tween-80 emulsifier to improve their dispersibility in water. During the addition process, the components are added slowly drop by drop while continuously stirring. The stirring speed is controlled at 300-400 rpm and the stirring time is not less than 30 minutes to ensure that all components are fully mixed and the solution does not have obvious stratification.
7. The method for preparing the broad-spectrum plant-derived disease resistance activator against rice blast as described in claim 1, characterized in that: In step S6, dilute hydrochloric acid or sodium hydroxide solution is used to adjust the pH value to a suitable range, usually 6.0-7.
0. During the adjustment process, the acid or alkali solution is added slowly while stirring to avoid over-addition. The adjusted solution is stirred again to ensure a uniform pH distribution.
8. The method for preparing a broad-spectrum plant-derived disease resistance activator against rice blast as described in claim 1, characterized in that: In step S7, the solution is heated to 60-70°C and held for 15-20 minutes, then slowly cooled to room temperature. Stabilizers, including xanthan gum or sodium carboxymethyl cellulose, are added to enhance the stability and suspension of the solution. After stabilization, the solution shows no obvious precipitation or stratification.
9. The method for preparing the broad-spectrum plant-derived disease resistance activator against rice blast as described in claim 1, characterized in that: In step S8, the mixed solution is filtered through a 0.22μm microporous membrane to remove any insoluble matter or impurities. During the filtration process, pressure-reducing filtration or peristaltic pumps are used to assist filtration to ensure the filtration effect; the filtered solution is clear and transparent, without suspended matter or sediment.
10. The method for preparing the broad-spectrum plant-derived disease resistance activator against rice blast as described in claim 1, characterized in that: In step S9, the product is stored in a cool, dry, and ventilated place, with the storage temperature controlled between 10-25℃, avoiding direct sunlight and high temperatures; the product is labeled with the production date, batch number, and instructions for use to ensure that the user uses it correctly.