Fixing device for small animal light irradiation

Abstract

The invention discloses a fixing device for small animal light irradiation. The fixing device comprises a fixing net, a storage plate, a connecting plate and a base plate. The fixing net is composed of warp rubber strips, weft rubber strips and clamping pieces fixed to the two ends of the weft rubber strips, wherein the warp rubber strips and the weft rubber strips are staggered. Adjusting holes and sliding lines are arranged on the clamping pieces in an array mode. Adaptive structures movably connected with the card adjusting holes are arranged on the edges of the two sides of the storage plate, nuts are embedded in the two corner ends of the storage plate respectively, and short screws and long screws are arranged at the two corner ends of the storage plate respectively; the short and long screws are in threaded connection with nuts; connecting and fixing devices are arranged at the adjacent side angle ends of the bottom face of the storage plate, the top face and the bottom face of the connecting plate and the top face of the base plate, so that adjacent plates are connected, and multi-degree-of-freedom angle adjustment is achieved. The fixing net is clamped with the convex column of the storage plate through the adjusting hole to control the tightness, and the warp / weft rubber strip forms a non-shielding light window in a target area through the movement of the circular ring; the height and the inclination angle of the storage plate are independently adjusted through short screws and long screws; the permanent magnet blocks are embedded in the base plate, and free splicing of a plurality of devices is supported.

Description

Technical Field

[0001] This invention belongs to the technical field of animal experimental equipment, and more specifically, relates to a fixation device for light irradiation of small animals. Background Technology

[0002] In photodynamic therapy (PDT) or phototherapy-related efficacy, toxicity, and pharmacokinetic studies, the energy distribution of the light source is significantly affected by distance, angle, and obstructions. For example, laser sources exhibit a scattering distribution centered on the fiber optic exit, while ultraviolet sources diffuse in a planar pattern centered on the lamp. According to the inverse square law of distance and Lambert's cosine law in photometry, the actual light energy density in the target area is inversely proportional to the square of the distance to the light source and decreases with the cosine of the incident angle. Currently, there are the following drawbacks when irradiating targets with light: 1) Insufficient individual adaptability: Differences exist in animal body shape (e.g., skeletal structure, fat distribution, surface curvature) or the condition of the target irradiation area (e.g., tumor protrusion height, growth angle). Traditional animal restraints are difficult to adjust in height and tilt, making the light dose received by the target area uncontrollable. Experimental data show that a height deviation of ±5mm can cause a light dose error of 17%~24% in the target area; illuminance is reduced to 86.6% when the light incident angle is ±30°, and illuminance is reduced by 50% when the incident angle is ±60°. 2) Severe occlusion interference: For example, patent CN103948451A discloses a restraint frame with a fixed mesh design. The fixed mesh leads to geometric occlusion (such as a theoretical occlusion rate of 9.75% when the mesh is 0.5mm in diameter and the spacing is 10mm) and mechanical marks. The latter can lead to temporary ischemia-reperfusion injury, causing inflammatory reactions such as erythema and edema. These symptoms are easily confused with chemical irritation or allergic reactions, causing false positive effects, and may affect drug metabolism assessment due to local ischemia.

[0003] 3) Low efficiency in multi-animal experiments: Animal experiments often involve a large number of animals. Irradiating each animal individually is time-consuming, and the light source weakens due to high temperatures, electrode sputtering, and gas consumption, making it difficult to compare results from different time periods. While patent CN205019600U supports simultaneous irradiation of multiple animals, it cannot independently adjust the position and angle of each individual animal. The light intensity of the beam often follows a Gaussian distribution, with the highest intensity at the center and exponentially decreasing at the edges. In multi-target irradiation scenarios, animals at different positions on the light spot receive different light doses, requiring adjustment of the device's height or angle for correction. Summary of the Invention

[0004] The present invention aims to provide a fixation device for light irradiation of small animals, which can not only adjust the height, angle, tightness and target light irradiation area, but also avoid obstruction, realizing multi-functional adjustment or correction. It solves the problem of inconsistent light dose or evaluation interference caused by the height and angle differences of the target irradiation area of ​​individual animals, as well as obstruction and marks.

[0005] To achieve the above objectives, the present invention provides the following technical solution: A light-irradiation fixation device for small animals includes a restraint net, a placement board, a connecting plate, and a base plate. The restraint net is disposed on the placement board, and the connecting plate is disposed between the placement board and the base plate. The Baoding net is composed of interlaced warp rubber strips, weft rubber strips, and cards fixed to both ends of the weft rubber strips. The cards are provided with an array of adjustment holes and sliding lines. The two sides of the shelf are provided with an adapter structure that is movably connected to the adjustment hole of the card. Nuts are fitted at both corners of the shelf, with a short screw at one corner and a long screw at the other corner; the short screw and the long screw are threadedly connected to the nuts. The bottom surface of the shelf, the top and bottom surfaces of the connecting plate, and the adjacent corner ends of the top surface of the base plate are all provided with connecting and fixing devices. Adjacent plates are connected and multi-degree-of-freedom angle adjustment is achieved through the connecting and fixing devices.

[0006] As an alternative example of the present invention, a pull line is also provided in the middle of the card in the middle section of the Baoding net. The pull line is connected to the sliding line. The local distribution density of the warp and weft rubber strips can be adjusted by moving the ring on the sliding line and the ring on the outermost weft rubber strip.

[0007] As an alternative example of the present invention, the two sides of the placement board are provided with symmetrically distributed protrusions, and the adjustment hole of the card is detachably connected to the protrusions of the placement board for adjusting the tension of the weft rubber band, fixing the animal and adjusting the animal's position.

[0008] As an alternative example of the present invention, the connecting and fixing device is an interlocking arc-shaped buckle structure.

[0009] As an alternative example of the present invention, the weft rubber strip is divided into a head segment, a neck segment, a thorax and abdomen segment, a pelvic segment and a tail root segment along the length of the animal's body, wherein the length of the thorax and abdomen segment is greater than that of the neck segment, which is greater than that of the pelvic segment, which is greater than that of the head segment, which is greater than that of the tail segment, and the corresponding card length and the number of adjustment holes decrease in that order.

[0010] As an alternative example of the present invention, a permanent magnet block is embedded on the upper surface of the connecting plate at the position corresponding to the short screw, and a hollow groove is provided at the corresponding position through which the middle long screw passes; the permanent magnet block of the connecting plate is magnetically fixed to the short screw of the shelf.

[0011] As an alternative example of the present invention, the base plate is a magnetically conductive metal plate, and permanent magnet blocks are embedded on its surface at positions corresponding to the long screws. The permanent magnet blocks of the base plate are magnetically fixed to the long screws of the shelf, and multiple base plates can be freely spliced ​​together through magnetic attraction.

[0012] As an alternative example of the present invention, the number of adjustment holes arranged in an array is 3; the number of warp rubber strips is 5 and the number of weft rubber strips is 18.

[0013] As an alternative example of the present invention, the card is a rigid card.

[0014] The beneficial effects of this invention are: 1) The small animal light irradiation fixing device of the present invention can adjust the height of the placement plate by simultaneously adjusting the length of the support plate supported by the short screw and the long screw; adjust the angle of the placement plate by adjusting the length of the support plate supported by the short screw or the long screw alone; adjust the position of the protruding post on both sides of the placement plate to lock into the adjustment hole on the card, so that the weft rubber strips at different parts of the animal's body are tightened to a certain extent, so as to achieve the purpose of comfortably and firmly fixing the animal; it can also adjust the animal's body position to ensure that the angle or direct relationship between the target irradiation area and the light source is adjustable and controllable; by adjusting the position of the end ring of each weft rubber strip on the sliding line, and the position of the end ring of each warp rubber strip on the outermost weft rubber strip, the warp and weft rubber strips are gathered to the non-irradiation area to form an adjustable-size unobstructed light window.

[0015] 2) The fixing device of the present invention is easy to assemble and disassemble, easy to clean, eliminates the interference of traditional mesh blockage and strangulation marks, realizes precise control of light source distance and incident angle, supports independent placement and removal of single device, and can be applied to efficacy, toxicity and pharmacokinetic tests related to laser photodynamic therapy (PDT) or photo-effects. Attached Figure Description

[0016] Figure 1 A schematic diagram of the connection structure of the placement plate, connecting plate and base plate in the fixing device for light irradiation of small animals provided by the present invention; Figure 2 This is a top view of the Baoding network. Figure 3 This is a top view of the shelf; Figure 4 This is a top view of the connecting plate; Figure 5 This is a top view of the base plate.

[0017] Reference numerals: 1-Baoding mesh; 2-Placement board; 3-Connecting plate; 4-Base plate; 11-Warp rubber strip; 12-Weft rubber strip; 13-Card; 14-Adjustment hole; 15-Sliding line; 16-Pull line; 17-Ring; 21-Nut; 22-Short screw; 23-Long screw; 24-Protruding column; 25-Curled structure; 31-Permanent magnet block; 32-Hollow groove. Detailed Implementation

[0018] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be emphasized that the specific embodiments described herein are only for better illustrating the invention and represent some, not all, embodiments, and therefore are not intended to limit the invention. Furthermore, the technical features involved in the embodiments of the invention described below can be combined with each other as long as they do not conflict with each other.

[0019] refer to Figures 1 to 5 As shown, an embodiment of the present invention provides a fixing device for light irradiation of small animals, including a restraining net 1, a placement plate 2, a connecting plate 3 and a base plate 4. The restraining net 1 is disposed on the placement plate 2, and the connecting plate 3 is disposed between the placement plate 2 and the base plate 4.

[0020] The shelf 2 has an adapter structure on both sides of its edge that is movably connected to the adjustment hole 14 of the card 13. For example, the adapter structure may be symmetrically distributed protrusions 24 on both sides of the shelf 2. The adjustment hole 14 of the card 13 is detachably connected to the protrusions 24 of the shelf 2, and is used to adjust the tension of the weft rubber band, fix the animal, and adjust the animal's position.

[0021] Specifically, nuts 21 are embedded at both corners of the shelf 2, with a short screw 22 at one corner and a long screw 23 at the other. The short screw 22 and the long screw 23 are threadedly connected to the nuts 21. A permanent magnet block 31 is embedded on the upper surface of the connecting plate 3 at the position corresponding to the short screw 22, and the permanent magnet block 31 of the connecting plate 3 is magnetically attracted and fixed to the short screw 22 of the shelf. A hollow groove 32 is provided at the corresponding position on the connecting plate 3 through which the long screw 23 passes. Preferably, the base plate 4 is a magnetically conductive metal plate, and a permanent magnet block 31 is embedded on its surface at the position corresponding to the long screw 23. The permanent magnet block 31 of the base plate 4 is magnetically attracted and fixed to the long screw 23 on the shelf.

[0022] The substrate of this invention adopts a magnetic design, which allows multiple substrates to be freely spliced ​​together through magnetic attraction, improving the flexibility and efficiency of the test.

[0023] like Figure 2As shown, the Baoding net 1 is composed of interlaced warp rubber strips 11, weft rubber strips 12, and cards 13 fixed at both ends of the weft rubber strips 12. The cards 13 are provided with arrayed adjustment holes 14 and sliding lines 15 passing through the adjustment holes 14.

[0024] The bottom surface of the shelf 2, the top and bottom surfaces of the connecting plate 3, and the adjacent corner ends of the base plate 4 are all equipped with connecting and fixing devices. Adjacent plates are connected and multi-degree-of-freedom angle adjustment is achieved through the connecting and fixing devices.

[0025] Preferably, the connecting and fixing device is an arc-shaped buckle structure 25 that can fit together, and adjacent plates can be connected and have multiple degrees of freedom angle adjustment through the interlocking arc-shaped buckle structure 25.

[0026] like Figure 2 As shown, a pull line 16 is also provided in the middle of the card 13 located in the middle section of Baoding Net 1. The pull line 16 is connected to the sliding line 15, and the local distribution density of the warp rubber strip 11 and the weft rubber strip 12 is adjusted by moving the ring 17 on the sliding line 15 and the ring 17 on the outermost weft rubber strip 12.

[0027] Specifically, the weft rubber band 12 can be divided into head segment, neck segment, thorax and abdomen segment, pelvic segment and tail root segment along the length of the animal's body. Among them, the length of the thorax and abdomen segment > neck segment = pelvic segment > head segment = tail segment, and the length of the corresponding card 13 and the number of adjustment holes 14 decrease in sequence.

[0028] For example, a set of arrayed adjustment holes 14 has 3 holes, warp rubber strips 11 has 5 strips, and weft rubber strips 12 has 18 strips.

[0029] Preferably, card 13 is a rigid card, and the shelf 2, connecting plate 3 and base plate 4 are rectangular in structure.

[0030] The small animal light irradiation fixation device provided by the present invention can adjust the height of the placement plate 2 by simultaneously adjusting the length of the support plate 2 of the short screw 22 and the long screw 23; adjust the angle of the placement plate 2 by adjusting the length of the support plate 2 of the short screw 22 or the long screw 23 alone; and adjust the position of the adjustment holes 14 on the card 13 by adjusting the protrusions 24 on both sides of the placement plate 2 so that the weft rubber strips 12 at different parts of the animal's body are tightened to a certain extent, so as to achieve the purpose of comfortably and firmly fixing the animal. It can also adjust the animal's body position to ensure that the angle or alignment between the target irradiation area and the light source is adjustable and controllable.

[0031] The method of using the light-illuminating fixation device for small animals is as follows: First, depending on the size of the animal, adjust the tightness of the restraint net 1 by adjusting the position of the protruding posts 24 on both sides of the placement plate 2 and the adjusting holes 14 on the card 13, ensuring that the animal can crawl into the spindle-shaped arch formed by the restraint net 1. When the animal is large, the card 13 near the animal's tail can be disengaged from the protruding posts 24 on both sides of the placement plate 2. After the animal crawls into the restraint net 1, small animals (such as mice) can choose the distal adjusting hole 14 of the card 13 to lock onto the protruding post 24 to achieve appropriate tension fixation, while large animals (such as rats) can choose the proximal hole position to achieve appropriate tension adaptation.

[0032] Then, by adjusting the appropriate positions of the protruding posts 24 on the placement plate 2 and the adjustment holes 14 on the corresponding card 13, the weft rubber strip 12 is tightened to a certain extent, so as to comfortably and firmly fix the animal's head, neck, chest, abdomen and tail base.

[0033] Furthermore, by simultaneously adjusting the position of the protruding posts 24 on both sides of the shelf 2 and the adjustment holes 14 on the card 13, the animal can be turned into a supine position, a right lateral position, a left lateral position, or a supine position, so that the target irradiation area is exposed to light.

[0034] When needed, the long screw 23 can be rotated counterclockwise while the short screw 22 is rotated clockwise to adjust the angle of the placement plate 2, and further adjust the illumination angle between the target illumination area on the animal's body and the light source. For example, raising the front of the placement plate 2 will make the animal tilt at a head-high-tail-low angle, and vice versa, so that the head-low-tail-high position is formed, allowing the light to illuminate the target area vertically or at a specific angle as needed.

[0035] Furthermore, the short screw 22 and the long screw 23 can be turned in the same direction to adjust the height of the placement plate 2 so that the target irradiation area on the animal's body is at the planned distance from the light source.

[0036] The present invention can adjust the position of the end ring 17 of each weft rubber strip 12 on the sliding line 15, and the position of the end ring 17 of each warp rubber strip 11 on the outermost weft rubber strip 12, so that the warp rubber strip 11 and the weft rubber strip 12 are gathered to the non-irradiation area, so that the target irradiation area forms an unobstructed window.

[0037] When multiple animals are irradiated simultaneously, multiple fixation devices can be freely spliced ​​together through edge magnetic attraction, with a magnetic attraction strength >0.3T. Fixation devices can also be added or removed at a certain time according to a plan, allowing for flexible and dynamic control of the irradiation time for each animal, thus improving the flexibility and efficiency of the experiment.

[0038] This invention provides a multifunctional small animal fixation device that integrates multi-degree-of-freedom body position adjustment, dynamic clearance window control, and modular combination functions. It is particularly suitable for local and systemic efficacy, phototoxicity, and metabolic experiments of animal laser photodynamic therapy (PDT) or light irradiation (such as ultraviolet B band) that require precise control of light dose and elimination of obstruction interference.

[0039] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the technical principles of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A device for fixing small animals to receive light, characterized in that, It includes a purlin (1), a shelf (2), a connecting plate (3) and a base plate (4), wherein the purlin (1) is disposed on the shelf (2) and the connecting plate (3) is disposed between the shelf (2) and the base plate (4); The Baoding net (1) is composed of interlaced warp rubber strips (11), weft rubber strips (12) and cards (13) fixed at both ends of the weft rubber strips (12). The cards (13) are provided with arrayed adjustment holes (14) and sliding lines (15). The two sides of the shelf (2) are provided with an adapter structure that is movably connected to the adjustment hole (14) of the card (13). Nuts (21) are respectively installed at the two corners of the shelf (2), with a short screw (22) at one corner and a long screw (23) at the other corner; the short screw (22) and the long screw (23) are threaded to the nut (21). The bottom surface of the shelf (2), the top and bottom surfaces of the connecting plate (3), and the adjacent corner ends of the base plate (4) are all provided with connecting and fixing devices. Adjacent plates are connected and multi-degree-of-freedom angle adjustment is achieved through the connecting and fixing devices.

2. The fixing device for light irradiation of small animals according to claim 1, characterized in that, A pull line (16) is also provided in the middle of the card (13) in the middle section of the Baoding net (1). The pull line (16) is connected to the sliding line (15). The local distribution density of the warp rubber strip (11) and the weft rubber strip (12) is adjusted by moving the ring (17) on the sliding line (15) and the ring (17) on the outermost weft rubber strip (12).

3. The fixing device for light irradiation of small animals according to claim 1, characterized in that, The two sides of the shelf (2) are provided with symmetrically distributed protrusions (24). The adjustment hole (14) of the card (13) is detachably connected to the protrusions (24) of the shelf (2) and is configured to adjust the tension of the weft rubber strip, fix the animal and adjust the animal's position.

4. The fixing device for light irradiation of small animals according to claim 1, characterized in that, The connecting and fixing device is an interlocking arc-shaped buckle structure (25).

5. The fixing device for light irradiation of small animals according to claim 1, characterized in that, The weft rubber strip (12) is divided into head segment, neck segment, thorax and abdomen segment, pelvic segment and tail root segment along the length of the animal body. The length of the thorax and abdomen segment is greater than that of the neck segment, which is greater than that of the pelvic segment, which is greater than that of the head segment, which is greater than that of the tail segment. The length of the corresponding card (13) and the number of adjustment holes (14) decrease in sequence.

6. The fixing device for light irradiation of small animals according to claim 1, characterized in that, The permanent magnet block (31) is embedded in the upper surface of the connecting plate (3) at the position corresponding to the short screw (22), and a hollow groove (32) is provided at the corresponding position through which the middle long screw (23) passes; the permanent magnet block (31) of the connecting plate (3) is magnetically fixed to the short screw (22) of the shelf.

7. The fixing device for light irradiation of small animals according to claim 1, characterized in that, The base plate (4) is a magnetically conductive metal plate, and a permanent magnet block (31) is embedded on its surface at the position corresponding to the long screw (23). The permanent magnet block (31) of the base plate (4) is magnetically fixed to the long screw (23) of the shelf, and multiple base plates can be freely spliced ​​through magnetic attraction.

8. The fixing device for light irradiation of small animals according to claim 1, characterized in that, The number of adjustment holes (14) arranged in an array is 3; the number of warp rubber strips (11) is 5 and the number of weft rubber strips (12) is 18.

9. The fixing device for light irradiation of small animals according to claim 1, characterized in that, The card (13) is a rigid card.

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