Automatic cleaning equipment for in-vitro tissue engineering skin

Abstract

The invention discloses in-vitro tissue engineering skin automatic cleaning equipment, and belongs to the technical field of cleaning equipment, the in-vitro tissue engineering skin automatic cleaning equipment comprises a cleaning tank, an ultrasonic cleaning module is arranged in the cleaning tank, the rear side of the cleaning tank is communicated with a liquid discharge pipe, a partition plate is mounted in an inner cavity of the cleaning tank, and the ultrasonic cleaning module is located at the top of the partition plate. According to the tissue engineering skin cleaning and rubbing device, the cleaning and rubbing effect on tissue engineering skin is improved through mutual circumferential movement of the pretreatment rollers on the two sides, the pretreated tissue skin falling into the surface of the conveying roller belt can be conveyed to the compaction mechanism on the rear side, and ultrasonic cleaning is conducted through ultrasonic waves emitted by the ultrasonic cleaning module in the cleaning tank in the compaction process; according to the in-vitro tissue engineering skin cleaning device, residual impurities of tissue skin are cleaned into a medium in the cleaning tank through ultrasonic waves, automatic cleaning of the in-vitro tissue engineering skin is achieved through pretreatment and compaction superposition cleaning, and cleaned materials can be conveyed to the tail section of the cleaning tank through a transmission roller to be conveniently taken and used.

Description

Technical Field

[0001] This invention belongs to the field of cleaning equipment technology, and in particular relates to an automatic cleaning device for isolated tissue-engineered skin. Background Technology

[0002] In clinical applications, tissue-engineered skin is often used for burn wound repair, ulcer healing, and drug testing platforms. During in vitro culture and subsequent applications, culture medium residues, cell debris, and impurities can easily adhere to the surface of tissue-engineered skin. If not thoroughly cleaned, these substances may affect tissue viability and clinical safety.

[0003] Existing technologies often use syringes or rinsing solutions to manually rinse tissue-engineered skin, which is inefficient, unstable, and prone to causing tissue damage. While ultrasonic vibrations can remove impurities, the single-action mode of ultrasound can lead to insufficient local cleaning, and the skin may curl and wrinkle during the cleaning process, affecting the cleaning effect. There is room for improvement. Summary of the Invention

[0004] The purpose of this invention is to provide an automated cleaning device for ex vivo tissue-engineered skin in order to solve the problems that the single action mode of ultrasound can easily lead to insufficient local cleaning and that the skin may curl and wrinkle during the cleaning process.

[0005] To achieve the above objectives, the present invention adopts the following technical solution:

[0006] An automated cleaning device for ex vivo tissue-engineered skin includes a cleaning tank, an ultrasonic cleaning module installed inside the cleaning tank, and a drain pipe connected to the rear side of the cleaning tank. A partition is installed inside the cleaning tank, and the ultrasonic cleaning module is located on top of the partition. The device also includes a pretreatment box connected to the top of the cleaning tank.

[0007] A pretreatment mechanism is connected to the top of the pretreatment box cavity. The pretreatment mechanism includes two pretreatment rollers that rotate in opposite directions. The pretreatment rollers are used to rub and remove impurities from the tissue skin.

[0008] The disturbance mechanism is located on one side of the pretreatment roller. The disturbance mechanism controls the pretreatment roller to move up and down in the treatment box to rub and remove impurities.

[0009] The compaction mechanism is located in the middle of the inner cavity of the cleaning tank. The compaction mechanism is located behind the pretreatment mechanism and flattens the tissue skin.

[0010] A conveyor belt is located below the bottom opening of the pretreatment box, with the end of the conveyor belt extending to the compaction mechanism. The conveyor belt transports the kneaded tissue skin to the compaction mechanism. A rinsing mechanism is located between the conveyor belt and the compaction mechanism to rinse the tissue skin.

[0011] As a further description of the above technical solution:

[0012] The pretreatment mechanism also includes:

[0013] A sliding plate, multiple sliding plates are arranged around the axis of the pretreatment roller, and the sliding plates are slidably connected to the groove opened on the outer periphery of the pretreatment roller;

[0014] Scraping blocks, multiple scraping blocks are slidably connected by sliders in a groove arrayed along the width direction of the sliding plate;

[0015] A buffer seat is slidably connected to the inner cavity of the pretreatment box. The inner side of the buffer seat is rotatably connected to the pretreatment roller. A drive unit is provided on the outside of the buffer seat. The output shaft of the drive unit is rotatably connected to the pretreatment roller, and the pretreatment roller is driven to rotate through the drive unit.

[0016] The first flushing nozzle, and multiple first flushing nozzles are arranged around the mounting groove opened on the outside of the pretreatment roller.

[0017] As a further description of the above technical solution:

[0018] It also includes: a fourth telescopic rod, the top end of which is connected to the bottom of the sliding plate, and the bottom end of which is connected to the outer groove of the pretreatment roller;

[0019] The fourth spring has its two ends connected to the corresponding positions on one side of the sliding plate and the inner cavity of the groove, respectively.

[0020] The fixed plates are connected to both sides of the buffer seat. The top of the fixed plate is connected to the first telescopic rod. The other end of the first telescopic rod is connected to the mounting plate. The other end of the mounting plate is connected to one side of the pretreatment box cavity. The first telescopic rod is fitted with a first spring. The two ends of the first spring are respectively connected to the corresponding positions of the mounting plate and the fixed plate.

[0021] As a further description of the above technical solution:

[0022] The disturbance mechanism includes:

[0023] The force-receiving plate has one end connected to one side of the buffer seat via a rod. The up-and-down movement of the force-receiving plate drives the buffer seat and the pretreatment roller to move longitudinally.

[0024] A movable rod is connected to an extrusion arc plate at its top. The movable rod is configured to move along the axis of the pretreatment roller. The lateral movement of the movable rod drives the contact between the extrusion arc plate and the force-receiving plate to control the movement of the buffer seat.

[0025] A force-bearing plate is connected to the end of a moving rod. A drive cam is in contact with the inner side of the force-bearing plate. A drive motor is connected to one side of the drive cam. The drive motor is connected to the outside of the pretreatment box.

[0026] A drive rod is connected to the end of the moving rod away from the force plate. One end of the drive rod is connected to a second telescopic rod. The other end of the second telescopic rod is connected to the inner cavity of the pretreatment box through a fixing block. A second spring is sleeved on the outside of the second telescopic rod. The two ends of the second spring are connected to the fixing block and one end of the drive rod, respectively.

[0027] As a further description of the above technical solution:

[0028] The compaction mechanism includes:

[0029] The first compaction roller belt and the second compaction roller belt are arranged perpendicularly and alternately. The first compaction roller belt is vertically connected to the cleaning tank, and the second compaction roller belt is fixed in the cleaning tank. The first compaction roller belt and the second compaction roller belt are located behind the conveyor roller belt. The tissue and skin are compacted by the opposite rotation of the first compaction roller belt and the second compaction roller belt.

[0030] As a further description of the above technical solution:

[0031] It also includes: mounting components, two mounting components connected to both sides of the first compaction roller belt, a third telescopic rod connected to both sides of the top of the mounting components, the other end of the third telescopic rod connected to the inner cavity of the cleaning tank through a connecting plate, a third spring sleeved on the outside of the third telescopic rod, the two ends of the third spring connected to the mounting components and the outside of the third telescopic rod respectively, and the first compaction roller belt is pulled to press against the second compaction roller belt through the third telescopic rod and the third spring.

[0032] As a further description of the above technical solution:

[0033] The rinsing mechanism includes:

[0034] A guide plate, wherein the guide plate is disposed at an inclined angle on the rear side of the conveyor belt;

[0035] The second flushing nozzles are arranged in an array along the width of the guide plate, and the second flushing nozzles are connected to each other through connecting pipes. The connecting sleeve extends to the outside of the cleaning tank and is connected to the pressure pump.

[0036] A baffle is connected to the top of the guide plate and to the inner cavity of the cleaning tank.

[0037] As a further description of the above technical solution:

[0038] The baffle is rotatably connected to a guide roller on one side of the conveyor belt, which guides the cleaned tissue skin to extend backward.

[0039] As a further description of the above technical solution:

[0040] Also includes:

[0041] The shielding plates are set at an angle at their ends, and the two shielding plates are connected to each other on both sides of the pretreatment chamber.

[0042] Multiple drive rollers are rotatably connected to the rear side of the cleaning tank in a double-row array, and the drive rollers are located behind the first compaction roller belt and the second compaction roller belt.

[0043] As a further description of the above technical solution:

[0044] Both sides of the cleaning tank are equipped with circulation pipes that extend to external filtration equipment to filter out impurities from ultrasonic cleaning.

[0045] In summary, due to the adoption of the above technical solution, the beneficial effects of the present invention are:

[0046] 1. In this invention, the pre-treatment mechanism is designed to place the tissue skin to be cleaned between adjacent pre-treatment mechanisms. The circumferential movement of the pre-treatment rollers on both sides improves the cleaning and rubbing effect on the tissue-engineered skin. The pre-treated tissue skin falling onto the surface of the conveyor roller can be sent to the rear compaction mechanism. During the compaction process, ultrasonic waves are emitted by the ultrasonic cleaning module in the cleaning tank to perform ultrasonic cleaning, and the remaining impurities of the tissue skin are cleaned into the medium in the cleaning tank by ultrasonic waves. Through the pre-treatment effect and the compaction superposition cleaning, the automatic cleaning of the ex vivo tissue-engineered skin is realized. The cleaned material can be transported to the end of the cleaning tank by the transmission roller for easy use.

[0047] 2. In this invention, through the designed compaction mechanism, when the pre-treated tissue-engineered skin is conveyed by the conveyor belt to the space between the first and second compaction roller belts on the rear side, the mutually rotating first and second compaction roller belts can fully compact the tissue skin to unfold the folds. Combined with the ultrasonic generator, this achieves thorough cleaning within the folds of the tissue skin, improving the cleaning effect. When the tissue-engineered skin is conveyed by the conveyor belt to the adjacent compaction mechanism, the guide plate can guide the tissue skin to the space between the adjacent first and second compaction roller belts. After guiding the tissue skin, the guide plate can rinse it through the second rinsing nozzles. The horizontally placed multiple second rinsing nozzles can improve the rinsing effect and ensure the cleaning effect.

[0048] 3. In this invention, through the designed disturbance mechanism, when the output shaft of the drive motor rotates, it can drive the cam to rotate. The rotation of the cam can squeeze the force plate to move. The movement of the force plate can drive the squeezing arc plate to squeeze the force plate. When the force plate is under pressure, it can drive the pretreatment roller to move upward. The pretreatment rollers on both sides can perform longitudinal kneading on the imported tissue-engineered skin. This is beneficial to achieve the clamping and scraping of the tissue skin through the interlaced pretreatment rollers. In addition, the longitudinally moving pretreatment rollers simulate longitudinal kneading to achieve thorough cleaning of the tissue skin. Attached Figure Description

[0049] Figure 1 This is a schematic diagram of the overall structure of an automated cleaning device for ex vivo tissue-engineered skin proposed in this invention.

[0050] Figure 2 This is a schematic diagram of the pretreatment mechanism of an automated cleaning device for ex vivo tissue-engineered skin proposed in this invention.

[0051] Figure 3 This is a schematic diagram of the pretreatment box assembly structure of an automated cleaning device for ex vivo tissue-engineered skin proposed in this invention;

[0052] Figure 4 This is a partially disassembled structural diagram of an automated skin cleaning device for ex vivo tissue-engineered skin proposed in this invention;

[0053] Figure 5 This is a side half-section diagram of an automated cleaning device for ex vivo tissue-engineered skin proposed in this invention;

[0054] Figure 6 The present invention proposes Figure 5 Enlarged structural diagram of section A;

[0055] Figure 7 This is a schematic diagram of the pretreatment mechanism of an automated cleaning device for ex vivo tissue-engineered skin proposed in this invention.

[0056] Figure 8 The present invention proposes Figure 7 Enlarged structural diagram of section B;

[0057] Figure 9 This is a schematic diagram of the rinsing mechanism of an automated cleaning device for ex vivo tissue-engineered skin proposed in this invention;

[0058] Figure 10 This is a schematic diagram of a pretreatment mechanism of an automated cleaning device for ex vivo tissue-engineered skin proposed in this invention.

[0059] Figure 11This is a schematic diagram of the disassembled structure of an automated skin cleaning device for ex vivo tissue-engineered skin proposed in this invention.

[0060] Legend:

[0061] 1. Cleaning tank; 2. Pretreatment box; 3. Shielding plate; 4. Pretreatment mechanism; 401. Pretreatment roller; 402. Sliding plate; 403. Scraper block; 404. Slider; 405. Mounting groove; 406. First rinsing nozzle; 407. Buffer seat; 408. Drive unit; 409. Fixing plate; 410. First telescopic rod; 411. First spring; 412. Mounting plate; 5. Disturbance mechanism; 501. Drive motor; 502. Drive cam; 503. Force plate; 504. Moving rod; 505. Drive rod 506. Second telescopic rod; 507. Second spring; 508. Fixing block; 509. Extrusion arc plate; 510. Force-receiving plate; 6. Compaction mechanism; 601. First compaction roller belt; 602. Second compaction roller belt; 603. Mounting component; 604. Third telescopic rod; 605. Third spring; 606. Connecting plate; 7. Conveyor roller belt; 8. Partition plate; 9. Flushing mechanism; 901. Guide plate; 902. Second flushing nozzle; 903. Connecting pipe; 904. Baffle plate; 905. Guide roller; 10. Transmission roller. Detailed Implementation

[0062] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0063] Please see Figures 1-11 The present invention provides a technical solution: an automatic cleaning device for ex vivo tissue-engineered skin, including a cleaning tank 1, an ultrasonic cleaning module is provided in the cleaning tank 1, and a drain pipe is connected to the rear side of the cleaning tank 1.

[0064] The inner cavity of the cleaning tank 1 is equipped with a partition 8, and the ultrasonic cleaning module is located on the top of the partition 8. Both sides of the cleaning tank 1 are provided with circulation pipes, which extend to external filtration equipment to filter out impurities under ultrasonic cleaning.

[0065] It also includes: a pretreatment box 2, which is connected to the top of the cleaning tank 1;

[0066] The pretreatment mechanism 4 is connected to the top of the inner cavity of the pretreatment box 2. The pretreatment mechanism 4 includes two pretreatment rollers 401 that rotate in opposite directions. The pretreatment rollers 401 are used to rub and remove impurities from the tissue skin.

[0067] The disturbance mechanism 5 is located on one side of the pretreatment roller 401. The disturbance mechanism 5 controls the pretreatment roller 401 to move up and down in the processing box to rub and remove impurities.

[0068] The compaction mechanism 6 is located in the middle of the inner cavity of the cleaning tank 1. The compaction mechanism 6 is located behind the pretreatment mechanism 4. The compaction mechanism 6 flattens the tissue skin.

[0069] The conveyor belt 7 is located below the bottom opening of the pretreatment box 2. The end of the conveyor belt 7 extends to the compaction mechanism 6. The rubbed tissue skin is conveyed to the compaction mechanism 6 through the conveyor belt 7. There is a rinsing mechanism 9 between the conveyor belt 7 and the compaction mechanism 6. The tissue skin is rinsed through the rinsing mechanism 9.

[0070] Specifically: Through the designed pretreatment mechanism 4, when the tissue skin needs to be treated, the tissue skin to be cleaned can be placed between adjacent pretreatment mechanisms 4. At this time, the two pretreatment rollers 401 move closer to each other to scrape off the residual tissue on the surface of the tissue-engineered skin. The mutual circumferential movement of the two pretreatment rollers 401 improves the cleaning and rubbing effect on the tissue-engineered skin. The pretreated tissue skin falling onto the surface of the conveyor roller belt 7 can be sent to the rear compaction mechanism 6. During the compaction process, the ultrasonic cleaning module in the cleaning tank 1 emits ultrasonic waves for ultrasonic cleaning, and the remaining impurities of the tissue skin are cleaned into the medium in the cleaning tank 1 by ultrasonic cleaning. This is beneficial to achieve automatic cleaning of the ex vivo tissue-engineered skin through the superposition of pretreatment effect and compaction cleaning. The cleaned material can be conveyed to the end of the cleaning tank 1 by the transmission roller 10 for easy use.

[0071] Please refer to the figure. Figures 2-3 and Figure 7 The pretreatment unit 4 also includes:

[0072] Sliding plate 402, multiple sliding plates 402 are arranged around the axis of pretreatment roller 401, and the sliding plates 402 are slidably connected to the groove opened on the outer periphery of the pretreatment roller 401;

[0073] Scraping blocks 403, multiple scraping blocks 403 are slidably connected to the sliding grooves arrayed along the width direction of the sliding plate 402 via sliders 404;

[0074] A buffer seat 407 is slidably connected to the inner cavity of the pretreatment box 2. The inner side of the buffer seat 407 is rotatably connected to the pretreatment roller 401. A drive unit 408 is provided on the outside of the buffer seat 407. The output shaft of the drive unit 408 is rotatably connected to the pretreatment roller 401. The drive unit 408 drives the pretreatment roller 401 to rotate.

[0075] First flushing nozzle 406, a plurality of first flushing nozzles 406 are arranged around the mounting groove 405 opened on the outside of the pretreatment roller 401;

[0076] It also includes: a fourth telescopic rod, the top end of which is connected to the bottom of the sliding plate 402, and the bottom end of which is connected to the outer groove of the pretreatment roller 401;

[0077] The fourth spring has its two ends connected to the corresponding positions on the sliding plate 402 and the inner cavity of the groove, respectively.

[0078] The fixing plate 409 is connected to both sides of the buffer seat 407. The top of the fixing plate 409 is connected to the first telescopic rod 410. The other end of the first telescopic rod 410 is connected to the mounting plate 412. The other end of the mounting plate 412 is connected to one side of the inner cavity of the pretreatment box 2. The first telescopic rod 410 is sleeved with a first spring 411. The two ends of the first spring 411 are respectively connected to the mounting plate 412 and the corresponding position on one side of the fixing plate 409.

[0079] Specifically: The first rinsing nozzle 406 is designed to spray rinsing medium outward when the pretreatment roller 401 rotates. The rinsing medium can be sprayed outward through the first rinsing nozzle 406 at an angle to the tissue and skin, thereby improving the rinsing cleaning angle. The pipe of the first rinsing nozzle 406 is connected to the external pumping equipment through a gas-liquid slip ring to ensure the connection effect.

[0080] Furthermore, through the design of the second telescopic rod 506 and the second spring 507, when the moving rod 504 moves, the second telescopic rod 506 can be pulled to unfold and the external second spring 507 can be pulled. The second spring 507 can use its own elasticity to maintain the stability of the moving rod 504, thereby improving the contact stability between the extrusion arc plate 509 and the force-bearing plate 510.

[0081] Please see Figure 7 and Figure 8 The disturbance mechanism 5 includes:

[0082] The force-receiving plate 510 has one end connected to one side of the buffer seat 407 via a rod. The up and down movement of the force-receiving plate 510 drives the buffer seat 407 and the pretreatment roller 401 to move longitudinally.

[0083] The moving rod 504 has an extrusion arc plate 509 connected to its top. The moving rod 504 is configured to move along the axis of the pretreatment roller 401. The lateral movement of the moving rod 504 drives the contact between the extrusion arc plate 509 and the force plate 510 to control the movement of the buffer seat 407.

[0084] The force plate 503 is connected to the end of the moving rod 504. The inner side of the force plate 503 contacts the drive cam 502. The drive cam 502 is connected to the drive motor 501 on one side. The drive motor 501 is connected to the outside of the pretreatment box 2.

[0085] A drive rod 505 is connected to the end of the moving rod 504 away from the force plate 503. One end of the drive rod 505 is connected to a second telescopic rod 506. The other end of the second telescopic rod 506 is connected to the inner cavity of the pretreatment box 2 through a fixing block 508. A second spring 507 is sleeved on the outside of the second telescopic rod 506. The two ends of the second spring 507 are respectively connected to the fixing block 508 and one end of the drive rod 505.

[0086] Specifically: Through the designed disturbance mechanism 5, when the output shaft of the drive motor 501 rotates, it can drive the cam to rotate. The rotation of the cam can squeeze the force plate 503 to move. The movement of the force plate 503 can drive the squeezing arc plate 509 to squeeze the force plate 510. When the force plate 510 is under pressure, it can drive the pretreatment roller 401 to move upward. The pretreatment rollers 401 that move longitudinally on both sides can perform longitudinal kneading on the imported tissue-engineered skin. This is beneficial for clamping and scraping the tissue skin through the interlaced pretreatment rollers 401. In addition, the longitudinally moving pretreatment rollers 401 simulate longitudinal kneading to achieve thorough cleaning of the tissue skin.

[0087] Furthermore, when the pretreatment roller 401 moves, it can press the first telescopic rod 410 by moving the fixed plates 409 on both sides. The first telescopic rod 410 can press the external first spring 411 by the force. The first spring 411 can absorb the vibration of the pretreatment roller 401 by its own elasticity, and provide stability for the longitudinal jump of the pretreatment roller 401.

[0088] Please see Figure 5 and Figure 6 The compaction mechanism 6 includes:

[0089] The first compaction roller belt 601 and the second compaction roller belt 602 are arranged perpendicularly to each other. The first compaction roller belt 601 is vertically connected to the cleaning tank 1, and the second compaction roller belt 602 is fixed in the cleaning tank 1. The first compaction roller belt 601 and the second compaction roller belt 602 are located behind the conveying roller belt 7. The tissue and skin are compacted by the opposite rotation of the first compaction roller belt 601 and the second compaction roller belt 602.

[0090] Mounting components 603 are connected to both sides of the first compaction roller belt 601. Each of the top two sides of the mounting components 603 is connected to a third telescopic rod 604. The other end of the third telescopic rod 604 is connected to the inner cavity of the cleaning tank 1 through a connecting plate 606. A third spring 605 is sleeved on the outside of the third telescopic rod 604. The two ends of the third spring 605 are respectively connected to the outer side of the mounting components 603 and the third telescopic rod 604. The first compaction roller belt 601 is pulled towards the second compaction roller belt 602 by the third telescopic rod 604 and the third spring 605.

[0091] Specifically: Through the designed compaction mechanism 6, when the pre-treated tissue-engineered skin is conveyed to the rear first compaction roller belt 601 and second compaction roller belt 602 via the conveyor roller belt 7, the mutually rotating first compaction roller belt 601 and second compaction roller belt 602 can fully compact the tissue skin to unfold the folds, and in conjunction with the ultrasonic generator, achieve thorough cleaning of the tissue skin folds, thereby improving the cleaning effect.

[0092] The first compaction roller belt 601 and the second compaction roller belt 602 are driven to rotate by their own drive units 408.

[0093] Please see Figure 5 and Figure 9 The rinsing mechanism 9 includes;

[0094] Guide plate 901 is inclined and located on the rear side of conveyor belt 7;

[0095] The second flushing nozzle 902, a plurality of second flushing nozzles 902 are arranged in an array along the width direction of the guide plate 901, and the plurality of second flushing nozzles 902 are connected to each other through a connecting pipe 903, and the connecting sleeve extends to the outside of the cleaning tank 1 and is connected to the pressure pump.

[0096] Baffle 904 is connected to the top of guide plate 901. Baffle 904 is connected to the inner cavity of cleaning tank 1. Baffle 904 is rotatably connected to guide roller 905 on one side of conveyor belt 7. Guide roller 905 guides the cleaned tissue skin to extend to the rear side.

[0097] It also includes: shielding plate 3, the ends of shielding plate 3 are set at an angle, and two shielding plates 3 are connected to each other on both sides of the inner cavity of the pretreatment box 2;

[0098] The drive rollers 10, a plurality of drive rollers 10 are rotatably connected to the rear side of the cleaning tank 1 in a double-row array, and the drive rollers 10 are located behind the first compaction roller belt 601 and the second compaction roller belt 602.

[0099] Specifically: Through the designed rinsing mechanism 9, when the tissue-engineered skin is conveyed to the adjacent compaction mechanism 6 via the conveyor roller belt 7, the guide plate 901 can guide the tissue skin between the adjacent first compaction roller belt 601 and second compaction roller belt 602. After guiding the tissue skin, the guide plate 901 can rinse it through the second rinsing nozzle 902. The horizontally placed multiple second rinsing nozzles 902 can improve the rinsing effect and ensure the cleaning effect.

[0100] In this invention, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance; the term "multiple" refers to two or more unless otherwise explicitly defined. The terms "installation," "connection," "linking," and "fixing," etc., should be interpreted broadly. For example, "connection" can be a fixed connection, a detachable connection, or an integral connection; "linking" can be a direct connection or an indirect connection through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0101] The above description is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.

Claims

1. An automatic cleaning device for ex vivo tissue engineering skin, comprising a cleaning tank (1), wherein an ultrasonic cleaning module is arranged in the cleaning tank (1), a liquid discharge pipe is connected to the rear side of the cleaning tank (1), and a partition plate (8) is arranged in the inner cavity of the cleaning tank (1), and the ultrasonic cleaning module is arranged on the top of the partition plate (8), characterized in that: a pretreatment box (2) is further arranged on the top of the cleaning tank (1); a pretreatment mechanism (4) is arranged on the top of the inner cavity of the pretreatment box (2), the pretreatment mechanism (4) comprises two pretreatment rollers (401) rotating towards each other, and the pretreatment rollers (401) are used for rubbing and removing impurities from the tissue skin; a disturbance mechanism (5) is arranged on one side of the pretreatment rollers (401), and the disturbance mechanism (5) is used for controlling the pretreatment rollers (401) to disturb and rub up and down in the pretreatment box to remove impurities; a compaction mechanism (6) is arranged in the middle of the inner cavity of the cleaning tank (1), and the compaction mechanism (6) is arranged on the rear side of the pretreatment mechanism (4), and is used for flattening the tissue skin; a conveying roller belt (7) is arranged below the opening in the bottom of the pretreatment box (2), the last segment of the conveying roller belt (7) extends to the compaction mechanism (6), the conveying roller belt (7) is used for conveying the tissue skin subjected to rubbing treatment to the compaction mechanism (6), and a flushing mechanism (9) is arranged between the gap between the conveying roller belt (7) and the compaction mechanism (6), and the flushing mechanism (9) is used for flushing the tissue skin. The pretreatment mechanism (4) further comprises: a plurality of sliding plates (402) are arranged around the axis of the pretreatment rollers (401), and the sliding plates (402) are slidingly connected to the groove bodies formed on the outer circumferential side of the pretreatment rollers (401); a plurality of scraping blocks (403) are slidingly connected to the slide grooves formed in the width direction of the sliding plates (402) through sliding blocks (404); a buffer seat (407) is slidingly connected to the inner cavity of the pretreatment box (2), the inner side of the buffer seat (407) is rotationally connected to the pretreatment rollers (401), a driving portion (408) is arranged on the outer side of the buffer seat (407), the output shaft of the driving portion (408) is rotationally connected to the pretreatment rollers (401), and the driving portion (408) is used for driving the pretreatment rollers (401) to rotate; and a plurality of first flushing nozzles (406) are arranged in the mounting grooves (405) formed on the outer side of the pretreatment rollers (401). Further comprising: a fourth telescopic rod, the top end of the fourth telescopic rod is connected to the bottom of the sliding plate (402), and the bottom end of the fourth telescopic rod is connected to the outer groove body of the pretreatment roller (401); and a fourth spring, the two ends of the fourth spring are connected to the corresponding positions on one side of the inner cavity of the sliding plate (402) and the groove body, respectively. ​ ​ ​ 2. The automatic cleaning device for ex vivo tissue engineered skin according to claim 1, characterized in that, ​ ​ ​ ​ ​ 3. The automatic cleaning apparatus for tissue engineered skin ex vivo according to claim 2, wherein ​ ​ ​ The fixed plate (409) connected to both sides of the buffer seat (407) is connected with the first telescopic rod (410) at the top, and the other end of the first telescopic rod (410) is connected with the mounting plate (412), and the other end of the mounting plate (412) is connected to one side of the inner cavity of the pretreatment box (2), and the first spring (411) is arranged outside the first telescopic rod (410), and the two ends of the first spring (411) are connected with the mounting plate (412) and the corresponding position of one side of the fixed plate (409) respectively.

4. The automatic cleaning apparatus for tissue engineered skin ex vivo according to claim 3, wherein, The perturbation mechanism (5) comprises: The force receiving disc (510) is connected to one side of the buffer seat (407) through a rod body, and the up-down movement of the force receiving disc (510) drives the longitudinal movement of the buffer seat (407) and the pretreatment roller (401); The moving rod (504) is connected with the extrusion arc plate (509) at the top, and the moving rod (504) is configured to move along the axis direction of the pretreatment roller (401), and the transverse movement of the moving rod (504) drives the extrusion arc plate (509) to contact the force receiving disc (510) to control the movement of the buffer seat (407); The force receiving plate (503) is connected to the end of the moving rod (504), and the drive cam (502) is in contact with the inner side of the force receiving plate (503), one side of the drive cam (502) is drivingly connected with the drive motor (501), and the drive motor (501) is connected to the outer side of the pretreatment box (2); The drive rod (505) is connected to the end of the moving rod (504) away from the force receiving plate (503), and the drive rod (505) is connected with the second telescopic rod (506) at one end, and the other end of the second telescopic rod (506) is connected to the inner cavity of the pretreatment box (2) through the fixed block (508), and the second spring (507) is arranged outside the second telescopic rod (506), and the two ends of the second spring (507) are connected with the fixed block (508) and the one end of the drive rod (505) respectively.

5. The automatic cleaning apparatus for tissue engineered skin ex vivo according to claim 1, wherein, The compaction mechanism (6) comprises: The first compaction roller belt (601) and the second compaction roller belt (602) are arranged vertically and staggered, the first compaction roller belt (601) is connected to the cleaning tank (1) in a lifting manner, the second compaction roller belt (602) is fixedly arranged in the cleaning tank (1), and the first compaction roller belt (601) and the second compaction roller belt (602) are located at the rear side of the conveying roller belt (7), and the skin tissue is compacted through the opposite rotation of the first compaction roller belt (601) and the second compaction roller belt (602).

6. The automatic cleaning apparatus for tissue engineered skin ex vivo according to claim 5, wherein, Further comprising: The installation piece (603) is connected to the two sides of the first compacting roller belt (601), and the top of the installation piece (603) is connected with a third telescopic rod (604) on both sides, the other end of the third telescopic rod (604) is connected to the inner cavity of the cleaning tank (1) through a connecting plate (606), the third telescopic rod (604) is externally sleeved with a third spring (605), and the two ends of the third spring (605) are respectively connected with the installation piece (603) and the outer side of the third telescopic rod (604), and the first compacting roller belt (601) is pulled to the second compacting roller belt (602) through the third telescopic rod (604) and the third spring (605).

7. The automatic cleaning apparatus for tissue engineered skin ex vivo according to claim 6, wherein, The flushing mechanism (9) comprises; A guide plate (901) is arranged on the rear side of the conveying roller belt (7) at an inclined angle; A plurality of second flushing nozzles (902) are arranged along the width direction of the guide plate (901), and the plurality of second flushing nozzles (902) are communicated through connecting pipes (903), and the connecting sleeves extend to the outside of the cleaning tank (1) and are communicated with the pressure pump; A baffle (904) is connected to the top of the guide plate (901), and the baffle (904) is connected to the inner cavity of the cleaning tank (1).

8. The automatic cleaning apparatus for tissue engineered skin ex vivo according to claim 7, wherein, The baffle (904) is rotatably connected with a guide roller (905) on one side corresponding to the conveying roller belt (7), and the guide roller (905) guides the washed tissue skin to extend to the rear side.

9. The automatic cleaning apparatus for tissue engineered skin ex vivo according to claim 1, wherein, Further comprising: A shielding plate (3) is arranged at an angle at the end, and two shielding plates (3) are connected to the inner cavities of the pretreatment boxes (2) on both sides; A plurality of transmission rollers (10) are rotatably connected to the rear side of the cleaning tank (1) in a double-row array, and the transmission rollers (10) are located on the rear side of the first compacting roller belt (601) and the second compacting roller belt (602).

10. The automatic cleaning apparatus for tissue engineered skin ex vivo according to claim 1, wherein, The cleaning tank (1) is provided with a circulating pipeline on both sides, the circulating pipeline extends to an external filtering device, and the impurities under ultrasonic cleaning are filtered through the circulating pipeline.

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