An auxiliary warming device for frozen embryo recovery

Abstract

This invention relates to the field of embryo resuscitation technology and discloses an auxiliary warming device for frozen embryo resuscitation, including a collection plate. A fixing ring is fixedly connected to the side wall of the collection plate, and a forward threaded groove is formed on the side wall of the fixing ring. The cover plate drives the separator ring to rotate clockwise through the contact rod and the fixing plate. Since the reverse threaded groove rotates in the opposite direction to the forward threaded groove, the rotating ring will move upward at this time. The upward rotating ring will drive the separator ring to move upward synchronously, so that the through hole gap coincides with the flow hole. At this time, the new solution on the outer wall of the separator ring flows downward through the flow hole and the through hole gap. Finally, the new solution mixes with the solution inside the collection plate. At this time, the pressure of the dripping will be absorbed by the top of the fixing ring and will not directly contact the solution inside the collection plate. When the operator twists the cover plate to close it, the flow hole and the through hole gap will coincide, and the new solution will mix with the solution inside the collection plate, effectively reducing the damage to the outer wall of the embryo cell membrane when injecting the new solution.

Description

Technical Field

[0001] This invention relates to the field of embryo resuscitation technology, specifically to an auxiliary warming device for resuscitating frozen embryos. Background Technology

[0002] Embryo cryopreservation methods have gradually shifted from slow freezing to vitrification. Vitrification uses a high-concentration cryoprotectant solution to directly transform cells from a liquid to a glassy state, thus preventing ice crystal formation. The thawing process after freezing is equally important. Thawing has been proven to be a multi-step warming process, gradually removing the cryoprotectant (CPA) through a series of solutions with decreasing osmotic pressure. Different concentrations of sucrose solutions (such as 1.0M and 0.5M) are used to control the rate of extracellular water diffusion and cell swelling, thereby improving embryo survival rates. Currently, various ready-made vitrification and thawing kits are available on the market. They are designed based on different sucrose concentration gradients to ensure optimal thawing results. For example, some warming kits contain 1.0M sucrose, 0.5M sucrose, and a separate HEPES buffer solution. Embryos are sequentially placed in different concentrations of sucrose solutions for several minutes. Each step aims to balance the osmotic pressure inside and outside the cells, safely displacing the cryoprotectant and removing it from the embryo while thawing.

[0003] The current mainstream heating method requires the use of multiple solutions for heating. First, the embryo is placed in a culture dish containing a solution, and then solutions of different concentrations are continuously added. During the dripping process, the ripples generated by the liquid droplets will be transmitted to the outer wall of the cell membrane. Since the cell membrane of the embryo is in a vitrified state, it is in a vulnerable state. Under the impact of the ripples, the cell membrane will be damaged. The conventional edge-dropping method can lead to infection of the tip of the dropper. To address the above problems, the following solution is proposed. Summary of the Invention

[0004] To solve the above-mentioned technical problems, the present invention provides an auxiliary warming device for frozen embryo thawing, comprising a collection plate, a fixing ring fixedly connected to the side wall of the collection plate, a forward threaded groove formed on the side wall of the fixing ring, a support plate fixedly connected to the bottom of the forward threaded groove, and a cover plate threadedly connected to the outer wall of the support plate, and further comprising:

[0005] The driving mechanism is fixedly connected to the side wall of the fixed ring;

[0006] The linkage mechanism is fixedly connected to the inner wall of the pushing mechanism;

[0007] The protective mechanism is slidably connected to the bottom of the linkage mechanism;

[0008] The components of the device are all made of glass. When using it, 0.05-0.1 ml of 1.0M sucrose needs to be injected into the collection plate first, and then the frozen embryos are placed inside the collection plate. In addition, during the operation, the temperature of all materials and solutions needs to be maintained at 37 degrees Celsius.

[0009] Preferred driving bodies include:

[0010] The separator assembly is fixedly connected to the side wall of the fixing ring;

[0011] The flow component is threadedly connected to the inner wall of the separator component;

[0012] Under normal circumstances, the cover plate needs to be screwed onto the top of the forward thread groove. When a new solution needs to be injected, the cover plate is rotated in the reverse direction to separate the forward thread groove from the cover plate. Then, the new solution is injected into the forward thread groove through a dropper, and then the cover plate is screwed onto the top of the support plate.

[0013] Preferably, the linkage mechanism includes:

[0014] The drive component is fixedly connected to the side wall of the flow component;

[0015] Misaligned component, the misaligned component is placed on the inner wall of the collection plate;

[0016] When the cover plate rotates, the cover plate drives the separating component and the flow component to rotate through the driving component.

[0017] Preferably, the protective mechanism includes:

[0018] The cover plate assembly is fixedly connected to the bottom of the misaligned assembly;

[0019] As the driving component moves the misaligned component upward, the misaligned component will cause the cover plate component to slide upward synchronously.

[0020] Preferably, the separating component includes a groove formed on the inner wall of the fixed ring, a partition ring fixedly connected to the top of the fixed ring, a flow hole formed on the side wall of the partition ring, and a reverse thread groove formed on the inner wall of the groove.

[0021] Among them, the forward thread groove and the reverse thread groove rotate in opposite directions. That is, when the cover plate rotates clockwise, the forward thread groove and the cover plate will gradually tighten, while when it rotates counterclockwise, the forward thread groove and the cover plate will gradually loosen.

[0022] Preferably, the flow assembly includes a rotating ring threadedly connected to the inner wall of the reverse thread groove, a partition ring fixedly connected to the top of the rotating ring, and a through hole slit provided on the side wall of the partition ring.

[0023] Under normal conditions, the flow hole and the through hole are misaligned, which prevents new solution on the periphery of the diaphragm from flowing inward.

[0024] Preferably, the drive assembly includes a push ring fixedly connected to the inner wall of the partition ring, a fixing plate fixedly connected to the top of the partition ring, and a contact rod fixedly connected to the bottom of the cover plate;

[0025] When the cover plate is placed on top of the forward threaded groove, it is necessary to ensure that both contact rods are inserted into the inner wall of the fixing plate.

[0026] Preferably, the cover plate assembly includes a frustum-shaped plate, a partition plate 2 is fixedly connected to the outer wall of the frustum-shaped plate, and four support frames are slidably connected to the inner wall of the through hole of the partition plate 2.

[0027] After placing the embryo on the inner wall of the collection plate, a second separator plate needs to be placed on the inner wall of the collection plate using sterile clips.

[0028] Preferably, the misalignment assembly includes a partition plate 1 fixedly connected to the top of the four support frames, and an L-shaped rod is fixedly connected to the top of the partition plate 1;

[0029] Under normal circumstances, the end of the L-shaped rod furthest from the partition plate should be placed on top of the push ring.

[0030] The present invention has the following beneficial effects:

[0031] (1) In view of the problem of embryo movement caused by the injection of new solution, when a new solution needs to be injected, the cover plate is rotated in the reverse direction to separate the forward thread groove from the cover plate, and then the new solution is injected through a dropper. Figure 3 At position G, after the new solution is evenly distributed on the outer wall of the spacer ring, the worker holds the cover plate and, after aligning it with the contact rod and fixing plate, rotates the cover plate clockwise, gradually tightening it with the forward thread groove. Simultaneously, the cover plate will cause the contact rod to rotate in the same direction. During this process, the cover plate, through the contact rod and fixing plate, causes the spacer ring to rotate clockwise. Since the reverse thread groove rotates in the opposite direction to the forward thread groove, when the rotating ring rotates clockwise, it will move upwards due to the influence of the reverse thread groove. This upward movement of the rotating ring will cause the spacer ring to move upwards synchronously, aligning the through-hole gap with the flow hole. Figure 5 The state changes to Figure 7In this state, the new solution on the outer wall of the septum flows downward through the gap between the flow hole and the through hole, and finally mixes with the solution inside the collection plate. Through the application of the above components, the new solution is first dripped onto position G. At this time, the pressure of the drip will be absorbed by the top of the fixing ring and will not come into direct contact with the solution inside the collection plate. Then, when the staff twists the cover to close it, the gap between the flow hole and the through hole will overlap, and the new solution will mix with the solution inside the collection plate, effectively reducing the damage to the outer wall of the embryonic cell membrane when injecting the new solution.

[0032] (2) This invention utilizes the characteristic that the flow hole and the through hole gap overlap, and designs the outlet of the through hole gap as a narrow opening, such as... Figure 7 The location of the through-hole slit greatly reduces the speed at which the new solution flows from top to bottom. As the new solution flows inward through the through-hole slit, it will flow downward along the inner wall of the collecting plate and mix with the old solution inside the collecting plate. In addition, after the new solution is dripped at position G, it will diffuse towards the outer wall of the septum and surround the bottom of the outer wall of the septum. After the through-hole slit coincides with the flow hole, the solution around it will flow downward through the through-hole slit. Through the application of the above components, the downward speed of the new solution is reduced, preventing the mixing speed from being too fast and causing turbulence inside the collecting plate, which would cause the embryo to impact the inner wall of the collecting plate.

[0033] (3) The present invention utilizes the feature that the above-mentioned separator ring will drive the push ring to slide up and down. The device is equipped with a misalignment component and a cover plate component. When the cover plate is spiraled upward to prepare for the injection of new solution, the push ring will slide downward. The push ring will drive the L-shaped rod to slide downward synchronously. At this time, the outer wall of the second separator plate will first contact the inner wall of the collection plate and restrict the downward movement of the second separator plate. As the push ring continues to move downward, the L-shaped rod will drive the first separator plate to continue to slide down. At this time, the first separator plate will drive the support frame to slide down along the through hole of the frustum plate. Finally, the first separator plate will completely cover the outer wall of the frustum plate. Through the application of the above components, when the device injects new solution into position G, the first separator plate and the frustum plate will cover the top of the old solution, reducing the contact area between the old solution and the air.

[0034] (4) This invention utilizes the characteristic of the push ring driving the partition plate to slide up and down. A partition plate and a support frame are installed inside the device. After the injection of a new solution is completed, as the screw thread of the cover plate rotates, it drives the push ring to slide upwards. During this process, the push ring, along with the L-shaped rod and the partition plate, slides upwards synchronously, causing the partition plate to move from... Figure 8 The state changes to Figure 10In this state, as new solution is continuously injected into the collecting plate, the increase in solution will eventually submerge the second partition plate. The first partition plate is pulled upward by the L-shaped rod, and then the frustum plate is moved upward by the support frame. This prevents the turbulence generated by the overall upward movement of the frustum plate from causing the solution inside the collecting plate to flow and causing the embryo to collide with the inside of the collecting plate. Attached Figure Description

[0035] To more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0036] Figure 1 This is a schematic diagram of the overall structure of the present invention;

[0037] Figure 2 This is a cross-sectional view of the overall structure of the present invention;

[0038] Figure 3 This is a cross-sectional schematic diagram of the separator component of the present invention;

[0039] Figure 4 This is a schematic cross-sectional view of the cover plate of the present invention;

[0040] Figure 5 For the present invention Figure 4 Enlarged view of point A in the middle;

[0041] Figure 6 This is a cross-sectional schematic diagram of the driving component of the present invention;

[0042] Figure 7 For the present invention Figure 6 Enlarged view of point B in the middle;

[0043] Figure 8 This is a cross-sectional schematic diagram of the misalignment component of the present invention;

[0044] Figure 9 This is a cross-sectional schematic diagram of the linkage mechanism of the present invention;

[0045] Figure 10 For the present invention Figure 9 Enlarged diagram of point C in the middle.

[0046] The attached diagram lists the components represented by each number as follows:

[0047] In the diagram: 1. Pushing mechanism; 11. Separating component; 12. Flow component; 13. Collecting plate; 14. Fixing ring; 15. Forward threaded groove; 16. Support plate; 17. Cover plate; 111. Slide groove; 112. Spacer ring; 113. Flow hole; 114. Reverse threaded groove; 121. Rotating ring; 122. Separating ring; 123. Through hole gap; 2. Linkage mechanism; 21. Drive component; 22. Misalignment component; 211. Pushing ring; 212. Fixing plate; 213. Contact rod; 221. L-shaped rod; 222. Separating plate one; 3. Protective mechanism; 31. Cover plate assembly; 311. Support frame; 312. Frustum-shaped plate; 313. Separating plate two. Detailed Implementation

[0048] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0049] Example 1, please refer to Figure 1 - Figure 6 This invention relates to an auxiliary warming device for frozen embryo thawing, comprising a collection plate 13, a fixing ring 14 fixedly connected to the side wall of the collection plate 13, a forward threaded groove 15 formed on the side wall of the fixing ring 14, a support plate 16 fixedly connected to the bottom of the forward threaded groove 15, and a cover plate 17 threadedly connected to the outer wall of the support plate 16, and further comprising:

[0050] A pushing mechanism 1 is fixedly connected to the side wall of the fixed ring 14;

[0051] Linkage mechanism 2 is fixedly connected to the inner wall of the pushing mechanism 1;

[0052] Protective mechanism 3 is slidably connected to the bottom of linkage mechanism 2;

[0053] The components of the device are all made of glass. When using it, 0.05-0.1 ml of 1.0M sucrose needs to be injected into the collection plate 13 first. It should be noted that the total amount of sucrose solution injected each time needs to be adjusted according to the size of the device. Then, the frozen embryo is placed inside the collection plate 13. In addition, during the operation, it is necessary to ensure that the temperature of all materials and solutions is maintained at 37 degrees Celsius.

[0054] The driving body 1 includes:

[0055] The partition component 11 is fixedly connected to the side wall of the fixing ring 14;

[0056] The flow component 12 is threadedly connected to the inner wall of the separator component 11;

[0057] Under normal conditions, the cover plate 17 is screwed onto the top of the forward threaded groove 15. When a new solution needs to be injected, the cover plate 17 is rotated in the reverse direction to separate the forward threaded groove 15 from the cover plate 17. Then, the new solution is injected into the forward threaded groove 15 through a dropper, and then the cover plate 17 is screwed onto the top of the support plate 16.

[0058] Linkage mechanism 2 includes:

[0059] Drive component 21 is fixedly connected to the side wall of flow component 12;

[0060] Misalignment component 22 is placed on the inner wall of collection plate 13;

[0061] When the cover plate 17 rotates, the cover plate 17 drives the separating component 11 and the flow component 12 to rotate via the driving component 21.

[0062] Protective mechanism 3 includes:

[0063] Cover plate assembly 31 is fixedly connected to the bottom of misalignment assembly 22;

[0064] As the driving component 21 moves the misalignment component 22 upward, the misalignment component 22 will drive the cover plate component 31 to slide upward synchronously.

[0065] Example 2, please refer to Figure 2 - Figure 10 The present invention is an auxiliary heating device for the revival of frozen embryos. Based on Example 1, the separating component 11 includes a groove 111 opened on the inner wall of the fixing ring 14, a partition ring 112 fixedly connected to the top of the fixing ring 14, a flow hole 113 opened on the side wall of the partition ring 112, and a reverse threaded groove 114 opened on the inner wall of the groove 111.

[0066] The forward thread groove 15 and the reverse thread groove 114 rotate in opposite directions. That is, when the cover plate 17 rotates clockwise, the forward thread groove 15 and the cover plate 17 will gradually tighten, while when it rotates counterclockwise, the forward thread groove 15 and the cover plate 17 will gradually loosen.

[0067] The flow assembly 12 includes a rotating ring 121 threadedly connected to the inner wall of the reverse thread groove 114, a partition ring 122 fixedly connected to the top of the rotating ring 121, and a through hole slit 123 opened on the side wall of the partition ring 122.

[0068] Under normal conditions, the flow hole 113 and the through hole gap 123 are misaligned, which prevents new solution located outside the spacer ring 112 from flowing inward.

[0069] The drive assembly 21 includes a push ring 211 fixedly connected to the inner wall of the partition ring 122, a fixing plate 212 fixedly connected to the top of the partition ring 122, and a contact rod 213 fixedly connected to the bottom of the cover plate 17.

[0070] Under normal conditions, the cover plate 17 is screwed onto the top of the forward-grooving groove 15. When a new solution needs to be injected, the cover plate 17 is rotated in the reverse direction to separate the forward-grooving groove 15 from the cover plate 17, and then the new solution is injected through a dropper. Figure 3 After the new solution is evenly distributed on the outer wall of the diaphragm ring 112, the staff holds the cover plate 17 and rotates it clockwise after aligning the contact rod 213 with the fixed plate 212, so that the cover plate 17 and the forward threaded groove 15 gradually become tighter.

[0071] The cover plate assembly 31 includes a frustum plate 312, a partition plate 313 is fixedly connected to the outer wall of the frustum plate 312, and four support frames 311 are slidably connected to the inner wall of the through hole of the partition plate 313.

[0072] In this process, the cover plate 17 drives the contact rod 213 to rotate in the same direction. The cover plate 17, through the contact rod 213 and the fixing plate 212, drives the separating ring 122 to rotate clockwise. Since the reverse thread groove 114 and the forward thread groove 15 rotate in opposite directions, when the rotating ring 121 rotates clockwise, it is affected by the reverse thread groove 114, causing the rotating ring 121 to move upwards. This upward movement of the rotating ring 121 drives the separating ring 122 to move upwards synchronously, causing the through hole gap 123 to coincide with the flow hole 113. Figure 5 The state changes to Figure 7 In this state, the new solution on the outer wall of the diaphragm 112 flows downward through the flow hole 113 and the through hole gap 123. Finally, the new solution mixes with the solution inside the collection plate 13. Through the application of the above components, the new solution is first dripped onto position G. At this time, the pressure of the drip will be absorbed by the top of the fixing ring 14 and will not directly contact the solution inside the collection plate 13. Subsequently, when the staff twists the cover plate 17 to close it, the flow hole 113 and the through hole gap 123 will overlap, and the new solution will mix with the solution inside the collection plate 13, effectively reducing the damage to the outer wall of the embryonic cell membrane when injecting the new solution.

[0073] The misalignment component 22 includes a partition plate 222 fixedly connected to the top of four support frames 311, and an L-shaped rod 221 fixedly connected to the top of the partition plate 222.

[0074] Utilizing the characteristic that the aforementioned separating ring 122 drives the pushing ring 211 to slide up and down, the device is equipped with a misalignment component 22 and a cover plate component 31. When the cover plate 17 is spiraled upward in preparation for injecting a new solution, the pushing ring 211 will slide downward. The pushing ring 211 will drive the L-shaped rod 221 to slide downward simultaneously. At this time, the outer wall of the second separating plate 313 will first contact the inner wall of the collecting plate 13, restricting the downward movement of the second separating plate 313. As the pushing ring 211 continues to move downward, the L-shaped rod 221 will drive the first separating plate 222 to continue to slide down. At this time, the first separating plate 222 will drive the support frame 311 to slide downward along the through hole of the frustum plate 312. Finally, the first separating plate 222 will completely cover the outer wall of the frustum plate 312. Through the application of the above components, when the device injects a new solution into position G, the first separating plate 222 and the frustum plate 312 will cover the top of the old solution, reducing the contact area between the old solution and the air.

[0075] One specific application of this embodiment is as follows: When using it, it is necessary to first inject enough 1.0M sucrose into the collection plate 13, and then place the frozen embryos into the collection plate 13. In addition, during the operation, it is necessary to ensure that the temperature of all materials and solutions is maintained at 37 degrees.

[0076] Under normal conditions, the cover plate 17 is screwed onto the top of the forward-threaded groove 15. When a new solution needs to be injected, the cover plate 17 is rotated in the reverse direction to separate the forward-threaded groove 15 from the cover plate 17. The new solution is then injected through a dropper. Figure 3 At position G, after the new solution is evenly distributed on the outer wall of the partition ring 112, the operator holds the cover plate 17 and, after aligning it with the contact rod 213 and the fixed plate 212, rotates the cover plate 17 clockwise, gradually tightening it with the forward thread groove 15. Simultaneously, the cover plate 17 drives the contact rod 213 to rotate in the same direction. During this process, the cover plate 17, through the contact rod 213 and the fixed plate 212, drives the partition ring 122 to rotate clockwise. Since the reverse thread groove 114 rotates in the opposite direction to the forward thread groove 15, when the rotating ring 121 rotates clockwise, it is affected by the reverse thread groove 114, causing the rotating ring 121 to move upwards. This upward movement of the rotating ring 121 drives the partition ring 122 to move upwards synchronously, causing the through hole gap 123 to coincide with the flow hole 113. Figure 5 The state changes to Figure 7In this state, the new solution on the outer wall of the diaphragm 112 flows downward through the flow hole 113 and the through hole gap 123. Finally, the new solution mixes with the solution inside the collection plate 13. Through the application of the above components, the new solution is first dripped onto position G. At this time, the pressure of the drip will be absorbed by the top of the fixing ring 14 and will not directly contact the solution inside the collection plate 13. Subsequently, when the staff twists the cover plate 17 to close it, the flow hole 113 and the through hole gap 123 will overlap, and the new solution will mix with the solution inside the collection plate 13, effectively reducing the damage to the outer wall of the embryonic cell membrane when injecting the new solution.

[0077] In this case, the position of each drop of the solution is... Figure 3 The position of G in the middle, and the position of G includes the entire inwardly inclined surface of the indicated position;

[0078] Taking advantage of the fact that the flow hole 113 and the through hole slit 123 coincide, the outlet of the through hole slit 123 is designed to be narrow, such as... Figure 7 The through-hole slit 123 position will greatly reduce the speed at which the new solution flows from top to bottom. When the new solution flows inward through the through-hole slit 123, the new solution will flow downward along the inner wall of the collection plate 13 and mix with the old solution inside the collection plate 13. In addition, after the new solution is dripped at position G, the new solution will diffuse towards the outer wall of the partition ring 112 and surround the bottom of the outer wall of the partition ring 112. After the through-hole slit 123 coincides with the flow hole 113, the solution around it will flow downward through the through-hole slit 123. Through the application of the above components, the downward speed of the new solution is reduced, and the mixing speed is prevented from being too fast, which would cause turbulence inside the solution inside the collection plate 13 and cause the embryo to impact the inner wall of the collection plate 13.

[0079] Utilizing the characteristic that the aforementioned separating ring 122 drives the pushing ring 211 to slide up and down, an offset component 22 and a cover plate component 31 are provided inside the device. When the cover plate 17 is spiraled upward in preparation for injecting a new solution, the pushing ring 211 will slide downward. The pushing ring 211 will drive the L-shaped rod 221 to slide downward simultaneously. At this time, the outer wall of the second separating plate 313 will first contact the inner wall of the collecting plate 13 and restrict the downward movement of the second separating plate 313. As the pushing ring 211 continues to move downward, the L-shaped rod 221 will drive the first separating plate 222 to continue to slide down. At this time, the first separating plate 222 will drive the support frame 311 to slide downward along the through hole of the frustum plate 312. Finally, the first separating plate 222 will completely cover the outer wall of the frustum plate 312. Through the application of the above components, when the device injects a new solution into position G, the first separating plate 222 and the frustum plate 312 will cover the top of the old solution, reducing the contact area between the old solution and the air.

[0080] Utilizing the characteristic of the aforementioned pushing ring 211 driving the partition plate 222 to slide up and down, a partition plate 222 and a support frame 311 are installed inside the device. After the injection of new solution, as the threads of the cover plate 17 rotate, the pushing ring 211 will slide upwards. During this process, the pushing ring 211, along with the L-shaped rod 221 and the partition plate 222, slide upwards synchronously, causing the partition plate 222 to... Figure 8 The state changes to Figure 10 In this state, as new solution is continuously injected into the collecting plate 13, the increase in solution will eventually submerge the second partition plate 313. The L-shaped rod 221 pulls the first partition plate 222 upward first, and then the support frame 311 drives the frustum plate 312 to move upward. This prevents the turbulence generated by the overall upward movement of the frustum plate 312 from causing the solution inside the collecting plate 13 to flow and causing the embryo to collide with the inside of the collecting plate 13.

[0081] The preferred embodiments of the present invention disclosed above are merely illustrative of the invention. These preferred embodiments do not exhaustively describe all details, nor do they limit the invention to the specific implementations described. Clearly, many modifications and variations can be made based on the content of this specification. This specification selects and specifically describes these embodiments to better explain the principles and practical applications of the invention, thereby enabling those skilled in the art to better understand and utilize the invention. The invention is limited only by the claims and their full scope and equivalents.

Claims

1. An auxiliary heating device for frozen embryo thawing, comprising a collection plate (13), wherein a fixing ring (14) is fixedly connected to the side wall of the collection plate (13), a forward threaded groove (15) is formed on the side wall of the fixing ring (14), a support plate (16) is fixedly connected to the bottom of the forward threaded groove (15), and a cover plate (17) is threadedly connected to the outer wall of the support plate (16), characterized in that, Also includes: A pushing mechanism (1) is fixedly connected to the side wall of a fixed ring (14); Linkage mechanism (2), which is fixedly connected to the inner wall of the pushing mechanism (1); The protective mechanism (3) is slidably connected to the bottom of the linkage mechanism (2); The components of the device are all made of glass. When using it, 0.05-0.1 ml of 1.0M sucrose needs to be injected into the collection plate (13) first, and then the frozen embryo is placed inside the collection plate (13). In addition, during the operation, it is necessary to ensure that the temperature of all materials and solutions is kept at 37 degrees. The propulsion mechanism (1) includes: A partition component (11) is fixedly connected to the side wall of the fixing ring (14); A flow component (12) is threadedly connected to the inner wall of the separator component (11); Under normal conditions, the cover plate (17) needs to be screwed onto the top of the forward thread groove (15). When a new solution needs to be injected, the cover plate (17) is rotated in the reverse direction to separate the forward thread groove (15) from the cover plate (17). Then, the new solution is injected into the forward thread groove (15) through a dropper, and then the cover plate (17) is screwed onto the top of the support plate (16). The linkage mechanism (2) includes: A drive assembly (21) is fixedly connected to the side wall of the flow assembly (12); Misalignment component (22), which is placed on the inner wall of the collection plate (13); When the cover plate (17) rotates, the cover plate (17) drives the partition component (11) and the flow component (12) to rotate through the drive component (21); The protective mechanism (3) includes: Cover plate assembly (31), which is fixedly connected to the bottom of the misalignment assembly (22); As the driving component (21) moves the misalignment component (22) upward, the misalignment component (22) will move the cover plate component (31) upward in sync. The separating component (11) includes a groove (111) formed on the inner wall of the fixing ring (14), a partition ring (112) is fixedly connected to the top of the fixing ring (14), a flow hole (113) is formed on the side wall of the partition ring (112), and a reverse thread groove (114) is formed on the inner wall of the groove (111). Among them, the forward thread groove (15) and the reverse thread groove (114) rotate in opposite directions. That is, when the cover plate (17) rotates clockwise, the forward thread groove (15) and the cover plate (17) will gradually tighten, while when it rotates counterclockwise, the forward thread groove (15) and the cover plate (17) will gradually loosen. The flow assembly (12) includes a rotating ring (121) threaded to the inner wall of the reverse thread groove (114), a partition ring (122) fixedly connected to the top of the rotating ring (121), and a through hole slit (123) opened on the side wall of the partition ring (122). Under normal conditions, the flow hole (113) and the through hole gap (123) are misaligned, which prevents new solution outside the partition ring (112) from flowing inward; The drive assembly (21) includes a push ring (211) fixedly connected to the inner wall of the partition ring (122).

2. The auxiliary warming device for thawing frozen embryos according to claim 1, characterized in that: A fixing plate (212) is fixedly connected to the top of the partition ring (122), and a contact rod (213) is fixedly connected to the bottom of the cover plate (17). When the cover plate (17) is placed on top of the forward threaded groove (15), it is necessary to ensure that both contact rods (213) are inserted into the inner wall of the fixing plate (212).

3. The auxiliary warming device for thawing frozen embryos according to claim 2, characterized in that: The cover plate assembly (31) includes a frustum plate (312), a partition plate (313) is fixedly connected to the outer wall of the frustum plate (312), and four support frames (311) are slidably connected to the inner wall of the through hole of the partition plate (313). After placing the embryo on the inner wall of the collection plate (13), the separator plate 2 (313) needs to be placed on the inner wall of the collection plate (13) using sterile clips.

4. The auxiliary warming device for thawing frozen embryos according to claim 3, characterized in that: The misalignment component (22) includes a partition plate (222) fixedly connected to the top of four support frames (311), and an L-shaped rod (221) is fixedly connected to the top of the partition plate (222). In normal conditions, the end of the L-shaped rod (221) away from the partition plate (222) needs to be placed on top of the push ring (211).

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