Detection device for solid-phase extraction, separation and purification of sample liquid

Abstract

The invention discloses a sample liquid solid phase extraction separation purification detection device, and relates to the technical field of food detection, the sample liquid solid phase extraction separation purification detection device comprises an outer protection assembly, a linkage assembly A and a linkage assembly B are installed in the outer protection assembly, a tray A and an inflation assembly are installed on the linkage assembly A, and a tray B and a tray C are installed on the linkage assembly B; the tray A and the tray B are both of gear-like structures, the tray A is meshed with the tray B, the linkage assembly A is used for driving the tray A to rotate and pulling the inflation assembly to ascend and descend, and the tray B can pull the tray C to rotate through the linkage assembly B. The linkage assembly is matched with the linkage assembly B, the inflation assembly can be pulled to extrude the liquid storage pipe to move downwards and assist in conveying a solution in the liquid storage pipe into the solid-phase extraction columns, and an extracting solution and a washing reagent can alternately pass through the corresponding solid-phase extraction columns, so that whole-process manual operation of experimenters is not needed, field waiting is not needed, and the working efficiency is improved. The sample liquid purification efficiency is effectively improved.

Description

Technical Field

[0001] This invention relates to biomass energy product testing services, and more particularly to the field of food testing technology, specifically a sample liquid solid phase extraction separation purification testing device. Background Technology

[0002] Food safety testing is a crucial measure to ensure that food does not pose a threat to consumer health during production, processing, storage, transportation, and sales. Food safety testing can identify and prevent foodborne illnesses, such as bacterial infections (e.g., Salmonella, E. coli) and chemical contamination (e.g., heavy metals, pesticide residues), thereby protecting consumer health. Through food safety testing, businesses can demonstrate the safety of their products to consumers, enhancing consumer confidence and promoting brand loyalty and market competitiveness. Food safety standards are a vital consideration in international food trade. Rigorous safety testing ensures that exported food meets the standards of importing countries, promoting the development of international trade. Continuous advancements and developments in food safety testing technology contribute to driving technological innovation and quality improvement throughout the food industry.

[0003] Food testing relies heavily on sample preparation. After simple preparation of the extract for food testing, researchers need to purify it to reduce impurities and improve the accuracy of the results. The purification process generally involves the following steps: First, the extract is overactivated using a solid-phase extraction (SPE) column. This allows the SPE column to selectively adsorb interfering impurities, while the analyte flows out; or it can simultaneously adsorb both impurities and the analyte. Then, a suitable solvent is used to selectively elute the analyte. Next, the sample solution flowing from the SPE column is concentrated, completely dried, and then reconstituted before being analyzed. This purified sample solution yields more accurate test results.

[0004] The above-mentioned operation method relies almost entirely on manual operation by laboratory personnel. The amount of sample solution that can be purified by the laboratory personnel at one time is very limited, and the time required for the solid phase extraction column to adsorb the target substance is relatively long. As a result, the efficiency of sample solution purification is low, which makes it difficult to meet the needs of large-scale food safety testing. Furthermore, when laboratory personnel perform purification of multiple samples at one time, the large number of reagents added and the overly complicated steps make it easy for operational errors to occur, leading to sample solution extraction failure and further affecting work efficiency. Summary of the Invention

[0005] The purpose of this application is to provide a sample liquid solid-phase extraction separation purification and detection device to solve the problems mentioned in the background art, which are that the number of sample liquids that the experimenter can purify at one time is very limited, and the waiting time for the solid-phase extraction column to adsorb the target substance is long. As a result, the work efficiency of sample liquid purification is low, which makes it difficult to meet the work needs of large-scale food safety testing. Moreover, when the experimenter performs purification of multiple sample liquids at one time, the addition of many types of reagents and the steps are too cumbersome, which easily leads to operational errors, resulting in sample liquid extraction failure and further affecting work efficiency.

[0006] To achieve the above objectives, this application provides the following technical solution: a sample liquid solid-phase extraction separation purification and detection device, comprising an outer protective component, wherein a linkage component A and a linkage component B are installed inside the outer protective component, a tray A and an inflation component are installed on the linkage component A, and a tray B and a tray C are installed on the linkage component B, wherein both tray A and tray B are gear-like structures and are meshed with each other, the linkage component A is used to drive the tray A to rotate and to pull the inflation component to rise and fall, the tray B can pull the tray C to rotate by means of the linkage component B, the outer protective component is used to wrap the tray A, tray B and tray C, a column is fixed at the center of the upper surface of the tray C, and a tray D is fixed at the top of the column, the tray D being located directly below the tray B.

[0007] Both tray A and tray B have a plurality of tube-dispensing holes A arranged in a circular array. A liquid storage tube is slidably inserted into each tube-dispensing hole A. A plurality of elastic lifting components are installed on the lower surface of tray A and tray B, with each elastic lifting component corresponding to one of the tube-dispensing holes A. The elastic lifting components are used to lift the liquid storage tube. A sealing plug A is inserted into the top of the liquid storage tube, and a through hole A is opened in the center of the sealing plug A. A liquid delivery needle is fixedly connected to the bottom of the liquid storage tube. The upper surface of tray C has a plurality of grooves arranged in a circular array, and a liquid receiving bottle is placed inside the grooves. Tray D has a plurality of tube-dispensing holes B arranged in a circular array. A solid-phase extraction column is slidably inserted into each tube-dispensing hole B. A sealing plug B is inserted into the top of the solid-phase extraction column, and a through hole B is opened in the center of the sealing plug B. The air-blowing component is used to inflate the liquid storage tube with air.

[0008] Furthermore, the external protection component includes a cabinet, with two cabinet doors hinged to both the front and rear sides of the cabinet, and handles fixed to the cabinet doors.

[0009] Furthermore, the linkage component A includes a drive motor, a damping shaft A, a carrier plate, and a guide rod. The drive motor, damping shaft A, carrier plate, and guide rod are all fixedly installed on the top of the cabinet interior. A driven shaft A is fixedly attached to the bottom of the damping shaft A. The bottom end of the driven shaft A is fixedly installed at the center of the tray A, and a grooved wheel is fixedly sleeved on the outside of the driven shaft A. The output shaft of the drive motor is connected to a transmission rod through a coupling. An active dial and a bevel gear A are fixedly sleeved on the outside of the transmission rod. A cylindrical pin is fixed on the active dial, and several radial grooves arranged in a circular array are opened on the grooved wheel.

[0010] Furthermore, a driven shaft B is inserted into the carrier plate, and the connection between the carrier plate and the driven shaft B is rotatably connected by a bearing. A bevel gear B is fixed to the tail end of the driven shaft B, and the bevel gear B meshes with the bevel gear A. A turntable is fixed to the front end of the driven shaft B, and an eccentric sleeve is rotatably connected to the turntable by a pin. A fixing plate is slidably sleeved on the outside of the guide rod. The inflation assembly is installed at the bottom of the fixing plate. A U-shaped seat is fixed to the upper surface of the fixing plate. A carrier tube is rotatably connected between the relative inner walls of the U-shaped seat by a pin. A connecting rod is fixed between the eccentric sleeve and the carrier tube.

[0011] Furthermore, the inflation assembly includes an air pump, which is fixedly installed at the bottom of the fixed plate. The air outlet of the air pump is fixedly connected to an air delivery needle, which is used to insert into the through hole A, and a pressure block is fixedly sleeved on the outside of the air delivery needle.

[0012] Furthermore, the elastic lifting assembly includes a carrier cylinder and a spring. The tops of the carrier cylinder and the spring are fixedly mounted on the lower surface of the tray A. A sliding rod is fixed to the bottom end of the spring. The carrier cylinder is sleeved on the outside of the sliding rod. A support plate is fixed to the bottom end of the sliding rod.

[0013] Furthermore, the tray has through holes for the liquid delivery needle to pass through.

[0014] Furthermore, the linkage component B includes a damping shaft B, a driven shaft C, and a damping shaft C. The upper and lower ends of the driven shaft C are rotatably connected to the inner wall of the cabinet via bearings. The damping shaft B is fixedly installed on the top of the inner side of the cabinet, and the damping shaft C is fixedly installed on the bottom of the inner side of the cabinet. A driven shaft D is fixedly installed at the bottom of the damping shaft B. The bottom end of the driven shaft D is fixedly installed at the center of the tray B, and a cylindrical gear A is fixedly sleeved on the outside of the driven shaft D. A cylindrical gear B and a disc are fixedly sleeved on the outside of the driven shaft C. The cylindrical gear A and the cylindrical gear B mesh with each other. A support column is fixedly installed on the top of the damping shaft C. The top end of the support column is fixedly installed at the center of the bottom of the tray C, and a cylindrical gear C is fixedly sleeved on the outside of the support column. Several sets of toothed columns are fixedly installed on the circumferential side of the disc. The several sets of toothed columns are distributed in a circular array, and all of the several sets of toothed columns mesh with the cylindrical gear C.

[0015] Furthermore, the cylindrical gear A and cylindrical gear B have the same number of teeth.

[0016] Furthermore, a base is installed at the bottom of the outer protective component, and a nitrogen blower, a gas chromatography-mass spectrometry (GC-MS) instrument, and a liquid chromatography-mass spectrometry (LC-MS) instrument are installed on the base.

[0017] In summary, the technical effects and advantages of this invention are as follows: 1. In this invention, the linkage component A is used to pull the intermittent rotation of tray A. Tray A and tray B are intermeshing with each other. Therefore, the linkage component A can indirectly pull the intermittent rotation of tray B and the linkage component B on tray B. The linkage component B can pull the rotation of tray C and tray D, and the rotation period of tray D is twice that of tray A. In this way, the liquid storage tubes placed on tray A and tray B can be alternately moved to the top of the solid phase extraction column placed on tray D. Under the traction of the linkage component A, the aeration component can be intermittently moved downward, squeezing the liquid storage tube downward and assisting the solution in the liquid storage tube to be transported into the solid phase extraction column. This allows the extract to alternately pass through the corresponding solid phase extraction column, and the solid phase extraction column can be rinsed. In this way, no manual operation by the experimenter is required throughout the process, and no on-site waiting is required. This effectively improves the efficiency of sample separation and purification, and better meets the needs of food testing.

[0018] 2. In this invention, the elastic lifting component can lift the liquid storage tube, so that the liquid delivery needle at the bottom of the liquid storage tube can only be pushed into the through hole B after the linkage component A pulls the inflation component downward. As the inflation component moves upward, the liquid storage tube will move upward and reset with the help of the elastic lifting component. This will not affect the alternation of the liquid storage tubes on tray A and tray B, which is conducive to the automated sample separation and purification work. In addition, the inflation component can pressurize the inside of the liquid storage tube, which helps the solution inside the liquid storage tube to be better injected into the solid phase extraction column. Under the action of air pressure, it can also help the sample liquid pass through the solid phase extraction column faster, thereby improving the efficiency of sample separation and purification.

[0019] 3. In this invention, a drive motor drives a transmission rod to rotate, which in turn pulls an active dial and a bevel gear A to rotate. During one revolution of the active dial, a cylindrical pin pulls a grooved wheel to rotate intermittently by one notch. The grooved wheel, via a driven shaft A, pulls tray A to rotate synchronously. Tray A and tray B mesh with each other, allowing the liquid storage tubes on trays A and B to alternately move above the solid-phase extraction column. This allows the extract to pass through the solid-phase extraction column first, followed by a rinse of the column with reagents, automatically completing the extraction of the target substance without secondary human intervention. Bevel gear A then pulls bevel gear B to rotate, which in turn pulls a turntable to rotate via a driven shaft B. The eccentric sleeve on the turntable rotates eccentrically, pushing a fixed plate up and down along a guide rod via a connecting rod. The fixed plate pulls the inflation assembly to move up and down accordingly, allowing the inflation assembly to pressurize the inside of the liquid storage tube, helping to better inject the solution into the solid-phase extraction column.

[0020] 4. In this invention, an air pump can be used to send air along the air delivery needle to the inside of the liquid storage tube, thereby increasing the air pressure inside the liquid storage tube. This facilitates the faster and better injection of the solution into the solid phase extraction column along the air delivery needle, thus accelerating the separation and purification of the sample solution.

[0021] 5. In this invention, the mutual meshing of tray A and tray B allows tray A to rotate synchronously with tray B when it rotates. Tray B, via driven shaft D, pulls cylindrical gear A to rotate, and cylindrical gear A pulls cylindrical gear B to rotate. This causes cylindrical gear B to pull a disc to rotate via driven shaft C. Several sets of teeth on the disc rotate accordingly, intermittently driving cylindrical gear C to rotate. This allows cylindrical gear C to intermittently pull tray C and tray D to rotate via support columns. As a result, the rotation period of tray D is twice that of tray A, facilitating the orderly injection of extractant and rinsing reagent by the solid-phase extraction column on tray D. Attached Figure Description

[0022] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the accompanying drawings used in the embodiments or the prior art will be briefly introduced below.

[0023] Figure 1 This is a three-dimensional structural schematic diagram of a sample liquid solid-phase extraction separation purification and detection device in an embodiment of this application; Figure 2 This is a schematic diagram of the external protection component and its internal structure in the embodiments of this application; Figure 3 Examples of embodiments in this application Figure 2 Partial structural diagram; Figure 4 This is a diagram showing the positional relationship between tray A, tray B, and linkage component A in the embodiments of this application; Figure 5 Examples of embodiments in this application Figure 4 Partial structural diagram; Figure 6 This is a diagram showing the connection relationship between some of the linkage components A and the inflation components in the embodiments of this application; Figure 7 Examples of embodiments in this application Figure 6 The right view of the structure; Figure 8 This is a diagram showing the positional relationship between tray C, liquid storage tube, liquid receiving bottle, and linkage component B in the embodiments of this application. Figure 9 Examples of embodiments in this application Figure 8 The front view of the structure; Figure 10 This is a diagram showing the positional relationship between the liquid storage tube, sealing plug A, liquid delivery needle, and elastic support assembly in the embodiments of this application. Figure 11 This is a diagram showing the positional relationship between tray C, column, tray D, and receiving bottle in an embodiment of this application.

[0024] In the diagram: 1. Tray A; 2. Tray B; 3. Tray C; 4. Column; 5. Tray D; 6. Tube discharge port A; 7. Liquid storage tube; 8. Sealing plug A; 9. Through hole A; 10. Liquid delivery needle; 11. Groove; 12. Receiving bottle; 13. Tube discharge port B; 14. Solid phase extraction column; 15. Sealing plug B; 16. Through hole B; 17. Cabinet; 18. Cabinet door; 19. Handle; 20. Drive motor; 21. Damping shaft A; 22. Driven shaft A; 23. Grooved wheel; 24. Transmission rod; 25. Drive dial; 26. Cylindrical pin; 27. Bevel gear A; 28. Carrier plate; 29. ​​Driven shaft B; 30. Conical... Gear B; 31. Turntable; 32. Eccentric sleeve; 33. Connecting rod; 34. Carrier tube; 35. U-shaped seat; 36. Fixing plate; 37. Guide rod; 38. Air pump; 39. Air needle; 40. Pressure block; 41. Carrier cylinder; 42. Spring; 43. Slide rod; 44. Support plate; 45. Through hole; 46. Damping shaft B; 47. Driven shaft C; 48. Damping shaft C; 49. Driven shaft D; 50. Cylindrical gear A; 51. Cylindrical gear B; 52. Disc; 53. Gear column; 54. Support column; 55. Cylindrical gear C; 56. Nitrogen blower; 57. Gas chromatography-mass spectrometry; 58. Liquid chromatography-mass spectrometry. Detailed Implementation

[0025] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments.

[0026] Example: Reference Figure 1-11The device shown includes an external protective assembly. Inside the external protective assembly are a linkage assembly A, a linkage assembly B, and a control module. Linkage assembly A has a tray A1 and an inflation assembly. After the system is programmed, the control module can control the orderly operation of linkage assembly A, linkage assembly B, and the inflation assembly. Linkage assembly B has trays B2 and C3. Trays A1 and B2 are gear-like structures and mesh with each other. Linkage assembly A drives tray A1 to rotate and pulls the inflation assembly up and down. Tray B2, with the help of linkage assembly B, can pull tray C3 to rotate. The external protective assembly encloses trays A1, B2, and C3. A column 4 is fixed at the center of the upper surface of tray C3, and a tray D5 is fixed at the top of column 4. Tray D5 is located directly below tray B2. Both trays A1 and B2 have multiple tube insertion holes A6 arranged in a circular array. A liquid storage tube 7 is slidably inserted into each tube insertion hole A6. The lower surface of tray A1 and tray B... Multiple elastic lifting components are installed on the lower surface of tray C2, and each elastic lifting component corresponds to a multiple tube discharge hole A6. The elastic lifting components are used to lift the liquid storage tube 7. A sealing plug A8 is inserted into the top of the liquid storage tube 7 to seal the top of the liquid storage tube 7. A through hole A9 is opened in the center of the sealing plug A8. A liquid delivery needle 10 is fixedly connected to the bottom of the liquid storage tube 7. The liquid delivery needle 10 has a small inner diameter and requires external pressure to dispense liquid. Multiple recesses are arranged in a circular array on the upper surface of tray C3. The groove 11 contains a receiving bottle 12. The tray D5 has multiple tube holes B13 arranged in a circular array. Solid phase extraction columns 14 are slidably inserted into the tube holes B13. The number of multiple liquid storage tubes 7 on the tray B2, the multiple receiving bottles 12 on the tray C3, and the multiple solid phase extraction columns 14 on the tray D5 are equal and their positions correspond. A sealing plug B15 is inserted into the top of the solid phase extraction column 14. A through hole B16 is opened in the center of the sealing plug B15. The air blowing assembly is used to fill the liquid storage tube 7 with air.

[0027] The outer protective component includes a cabinet 17, with two cabinet doors 18 hinged to the front and rear sides of the cabinet 17. Each cabinet door 18 has a handle 19 fixed on it. Both the front and rear cabinet doors 18 can be opened to facilitate the placement and removal of the liquid storage tube 7, the liquid receiving bottle 12, and the solid phase extraction column 14.

[0028] The linkage component A includes a drive motor 20, a damping shaft A21, a carrier plate 28, and a guide rod 37. The drive motor 20, damping shaft A21, carrier plate 28, and guide rod 37 are all fixedly installed on the top of the inner side of the cabinet 17. A driven shaft A22 is fixedly installed at the bottom of the damping shaft A21. The bottom end of the driven shaft A22 is fixedly installed at the center of the tray A1, and a grooved wheel 23 is fixedly sleeved on the outside of the driven shaft A22. The output shaft of the drive motor 20 is connected to the transmission rod 24 via a coupling. The transmission rod 24 is externally fixed with the drive dial 25 and the bevel gear A27. The drive dial 25 is fixed with a cylindrical pin 26. The grooved wheel 23 has multiple radial grooves arranged in a circular array. The drive motor 20 drives the transmission rod 24 to rotate, and the transmission rod 24 pulls the drive dial 25 and the bevel gear A27 to rotate. When the cylindrical pin 26 rotates one revolution with the drive dial 25, it can cooperate with the radial grooves on the grooved wheel 23 to drive the grooved wheel 23 to rotate one position. The grooved wheel 23 can pull the tray A1 to rotate synchronously with it via the driven shaft A22.

[0029] The carrier plate 28 has a driven shaft B29 inserted into it, and the connection between the carrier plate 28 and the driven shaft B29 is rotatably connected by a bearing. A bevel gear B30 is fixed to the tail end of the driven shaft B29, and the bevel gear B30 meshes with the bevel gear A27. A turntable 31 is fixed to the front end of the driven shaft B29, and an eccentric sleeve 32 is rotatably connected to the turntable 31 via a pin. A fixing plate 36 is slidably fitted onto the outside of the guide rod 37. The inflation assembly is installed at the bottom of the fixing plate 36. A U-shaped seat 35 is fixed to the upper surface of the fixing plate 36, and a carrier tube 34 is rotatably connected between the relative inner walls of the U-shaped seat 35 via a pin. A connecting rod 33 is fixed between the eccentric sleeve 32 and the carrier tube 34. Tray A1 and tray B2 mesh with each other. The liquid storage tubes 7 on trays A1 and B2 can be alternately moved above the solid-phase extraction column 14, so that the extract first passes through the solid-phase extraction column 14, and then the reagent rinses the solid-phase extraction column 14, automatically completing the extraction of the target substance without secondary human intervention. The bevel gear A27 can drive the bevel gear B30 to rotate, and the bevel gear B30 drives the turntable 31 to rotate via the driven shaft B29. The eccentric sleeve 32 on the turntable 31 rotates eccentrically, and can push the fixing plate 36 up and down along the guide rod 37 via the connecting rod 33. The fixing plate 36 drives the aeration component to move up and down accordingly, so that the aeration component can inflate and pressurize the inside of the liquid storage tube 7, helping the solution inside the liquid storage tube 7 to be better injected into the solid-phase extraction column 14.

[0030] The inflation assembly includes an air pump 38, which is fixedly installed at the bottom of the fixed plate 36. The air outlet of the air pump 38 is fixedly connected to an air delivery needle 39, and the air inlet is equipped with a filter screen to block the entry of impurities. The air delivery needle 39 is inserted into the through hole A9, and a pressure block 40 is fixedly sleeved on the outside of the air delivery needle 39. The air pump 38 can send air along the air delivery needle 39 to the inside of the liquid storage tube 7, thereby increasing the air pressure inside the liquid storage tube 7. This facilitates the faster and better injection of the solution in the liquid storage tube 7 into the solid phase extraction column 14 along the liquid delivery needle head 10.

[0031] The elastic support assembly includes a carrier cylinder 41 and a spring 42. The tops of the carrier cylinder 41 and the spring 42 are fixedly mounted on the lower surface of the tray A1. A sliding rod 43 is fixed to the bottom end of the spring 42. The carrier cylinder 41 is sleeved on the outside of the sliding rod 43. A support plate 44 is fixed to the bottom end of the sliding rod 43. The support plate 44 has a through hole 45 for the liquid delivery needle 10 to pass through. The support plate 44 supports the liquid storage tube 7. When the inflation assembly moves down and squeezes the liquid storage tube 7 down, the liquid delivery needle 10 at the bottom of the liquid storage tube 7 can be pushed into the through hole B16. After the inflation assembly moves up, the liquid storage tube 7 can be moved up and reset by the elastic force of the spring 42.

[0032] The linkage component B includes a damping shaft B46, a driven shaft C47, and a damping shaft C48. The upper and lower ends of the driven shaft C47 are rotatably connected to the inner wall of the cabinet 17 via bearings. The damping shaft B46 is fixedly installed on the top of the inner side of the cabinet 17, and the damping shaft C48 is fixedly installed on the bottom of the inner side of the cabinet 17. A driven shaft D49 is fixedly installed at the bottom of the damping shaft B46. The bottom end of the driven shaft D49 is fixedly installed at the center of the tray B2, and a cylindrical gear A is fixedly sleeved on the outside of the driven shaft D49. 50. A cylindrical gear B51 and a disk 52 are fixedly sleeved on the outside of the driven shaft C47. The cylindrical gear A50 meshes with the cylindrical gear B51. A support column 54 is fixedly fixed to the top of the damping shaft C48. The top of the support column 54 is fixedly installed at the center of the bottom of the tray C3, and a cylindrical gear C55 is fixedly sleeved on the outside of the support column 54. Multiple sets of toothed columns 53 are fixedly installed on the circumferential side of the disk 52. The multiple sets of toothed columns 53 are distributed in a circular array, and all of the multiple sets of toothed columns 53 mesh with the cylindrical gear C55. All three gears—spur gear A50, spur gear B51, and spur gear C55—have the same number of teeth. The total number of multiple sets of gear spurs 53 is half the number of teeth in spur gear C55. The remaining clearance on the circumferential side of the disk 52 between two adjacent sets of gear spurs 53 can accommodate exactly the same number of teeth as one set of gear spurs 53. Through the meshing of trays A1 and B2, tray A1 rotates, pulling tray B2 to rotate synchronously. Tray B2, via driven shaft D49, pulls spur gear A50 to rotate accordingly. Wheel A50 pulls spur gear B51 to rotate, which in turn pulls disk 52 to rotate via driven shaft C47. Multiple sets of toothed columns 53 on disk 52 rotate accordingly, which can intermittently drive spur gear C55 to rotate. This causes spur gear C55 to intermittently pull tray C3 and tray D5 to rotate via support column 54. This results in the rotation cycle of tray D5 being twice that of tray A1, which facilitates the orderly injection of extract and rinsing reagent by solid phase extraction column 14 on tray D5. The outer protective component has a base installed at the bottom, on which a nitrogen blower 57, a gas chromatography-mass spectrometry (GC-MS) instrument 58, and a liquid chromatography-mass spectrometry (LC-MS) instrument 59 are installed. The nitrogen blower 57 is used to concentrate the solution ultimately carried by the liquid bottle 12. The reconstituted sample solution can be used for component detection by the GC-MS instrument 58 or the LC-MS instrument 59.

[0033] Working principle of this invention: The experimenters placed different food extracts into multiple storage tubes 7. Then, based on the type of target substance in the extract, they prepared a corresponding amount of rinsing reagent and placed it into several additional storage tubes 7. A sealing plug A8 was installed on the top of each storage tube 7 to seal it. Subsequently, the storage tubes 7 containing the extract were placed one by one into the multiple tube insertion holes A6 on tray A1, and the storage tubes 7 containing the rinsing reagent were placed one by one into the multiple tube insertion holes A6 on tray B2, ensuring that the storage tubes 7 on tray B2 corresponded to those on tray A1. The tubes were then removed from the trays. Multiple solid-phase extraction columns 14, matching the number of liquid storage tubes 7 on tray A1, are selected according to the different target substances. The sealing plug B15 is installed on the top of the solid-phase extraction column 14 to seal the top of the solid-phase extraction column 14. Then, multiple solid-phase extraction columns 14 are placed one by one in the multiple tube holes B13 on tray D5. Finally, multiple liquid receiving bottles 12 corresponding to the number of solid-phase extraction columns 14 are taken out and placed in the multiple grooves 11 on tray C3. The multiple liquid storage tubes 7 on tray B2, the multiple solid-phase extraction columns 14 on tray D5, and the multiple liquid receiving bottles 12 on tray C3 are all placed accordingly. During operation, the experimenter controls the drive motor 20 via the control module to operate as needed, causing the drive motor 20 to intermittently drive the transmission rod 24 to rotate half a revolution. The interval between half revolutions is the total time taken for the aeration component to send the solution in the storage tube 7 to the solid-phase extraction column 14 and for the solution to flow out. The amounts of extract and rinsing reagent added are predetermined values ​​during the experiment, so their impact on the total time is negligible. During the half revolution of the drive motor 20 driving the transmission rod 24, the transmission rod 24 can pull the active dial 25 and the bevel gear A27 to rotate half a revolution. The cylindrical pin 26 on the active dial 25 engages with the radial groove on the grooved wheel 23, which can pull the grooved wheel 23 to rotate one notch and then stop moving. Tray A1 and tray B2 mesh with each other, and the rotating tray A1 can pull the tray B2 to rotate one notch accordingly. At this time, the storage tube 7 on tray A1 rotates to directly below the aeration component and then stops moving. Tray B2, with the help of the linkage component B, pulls tray C3 and tray D5 to rotate one notch and then stops moving. As the transmission rod 24 continues to rotate, the bevel gear A27 pulls the bevel gear B30 to rotate. The bevel gear B30, in turn, pulls the turntable 31 to rotate via the driven shaft B29. The turntable 31 pulls the eccentric sleeve 32 to rotate eccentrically. The eccentric sleeve 32, through the connecting rod 33, pushes the fixing plate 36 downward along the guide rod 37. The inflation assembly follows the fixing plate 36 downward, which can cause the air needle 39 to insert into the through hole A9. As the inflation assembly continues to move downward, the pressure block 40 can squeeze the liquid storage tube 7 downward, causing the liquid storage tube 7 to... The push plate 44 moves down, forcing the spring 42 to extend. As a result, the liquid delivery needle 10 will be inserted into the through hole B16. The control module controls the air pump 38 to run, so that the air pump 38 fills the liquid storage tube 7 with air, forcing the air pressure inside the liquid storage tube 7 to increase. The extract inside can flow into the solid phase extraction column 14. Under the action of air pressure, after the extract flows through the solid phase extraction column 14, the target substance and impurities or pure impurities are adsorbed by the solid phase extraction column 14, and a small amount of unaffected liquid flows into the receiving bottle 12. Next, the control module controls the drive motor 20 to drive the transmission rod 24 to rotate half a turn again, so that the inflation component moves up and resets, and the liquid storage tube 7 on tray A1 moves up and resets under the elastic force of spring 42. At this time, tray A1, tray B2, tray C3 and tray D5 do not rotate. Subsequently, the control module controls the drive motor 20 to drive the transmission rod 24 to rotate half a turn. At this time, tray A1 and tray B2 rotate one unit again, while tray C3 and column 4 do not rotate. During this half-turn, the aeration component will help the reagent in the liquid storage tube 7 on tray B2 to be introduced into the solid phase extraction column 14 until the target substance completely flows into the receiving bottle 12. Afterwards, the control module controls the drive motor 20 to drive the transmission rod 24 to rotate half a turn again, so that the inflation component moves up and resets, and the liquid storage tube 7 on tray B2 is reset. At this time, trays A1, B2, C3 and D5 all rotate one unit to enter the next liquid separation and purification. After all sample solutions have been separated and purified, the experimenter removes all the liquid storage tubes 7, solid-phase extraction columns 14, and receiving bottles 12 from the outer protective assembly. After all the receiving bottles 12 are removed in an orderly manner, they are placed in the nitrogen evaporator 57 for concentration. After concentration, all the receiving bottles 12 are removed from the nitrogen evaporator 57 in an orderly manner. A quantitative amount of solvent is used to remelt the concentrated solids using a pipette. A certain amount of the remelted sample solution is transferred to the injection vial using a pipette. Depending on the different components to be detected in the sample solution, the injection vial containing the sample solution is placed in the gas chromatography-mass spectrometry (GC-MS) instrument 58 or the liquid chromatography-mass spectrometry (LC-MS) instrument 59 to complete the on-machine detection, obtain the test results, and determine whether the food is qualified.

[0034] Finally, it should be noted that the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A sample liquid solid-phase extraction separation purification and detection device, characterized in that: The application relates to an external protection assembly, a linkage assembly A and a linkage assembly B are arranged in the internal part of the external protection assembly, a tray A (1) and an inflation assembly are arranged on the linkage assembly A, a tray B (2) and a tray C (3) are arranged on the linkage assembly B, the tray A (1) and the tray B (2) are both gear-like structures, the tray A (1) and the tray B (2) are meshed with each other, the linkage assembly A is used for driving the tray A (1) to rotate and dragging the inflation assembly to lift, the tray B (2) can drag the tray C (3) to rotate through the linkage assembly B, the external protection assembly is used for wrapping the tray A (1), the tray B (2) and the tray C (3), a stand (4) is fixed at the center of the upper surface of the tray C (3), a tray D (5) is fixed at the top of the stand (4), and the tray D (5) is located directly below the tray B (2). A plurality of pipe placing holes A (6) in circular array are arranged on the tray A (1) and the tray B (2), a liquid storage pipe (7) is slidably inserted into the pipe placing hole A (6), a plurality of elastic lifting assemblies are arranged on the lower surface of the tray A (1) and the lower surface of the tray B (2), the elastic lifting assemblies are arranged in one-to-one correspondence with the pipe placing holes A (6), and the elastic lifting assemblies are used for lifting the liquid storage pipe (7), a sealing plug A (8) is inserted into the top of the liquid storage pipe (7), a through hole A (9) is formed in the center of the sealing plug A (8), a liquid delivery needle (10) is fixedly connected to the bottom of the liquid storage pipe (7), a plurality of grooves (11) in circular array are arranged on the upper surface of the tray C (3), a liquid receiving bottle (12) is arranged in the groove (11), a plurality of pipe placing holes B (13) in circular array are arranged on the tray D (5), a solid phase extraction column (14) is slidably inserted into the pipe placing hole B (13), a sealing plug B (15) is inserted into the top of the solid phase extraction column (14), a through hole B (16) is formed in the center of the sealing plug B (15), and the inflation assembly is used for inflating the liquid storage pipe (7).

2. The sample liquid-solid phase extraction separation and purification detection device according to claim 1, characterized in that: The external protection assembly comprises a cabinet body (17), two cabinet doors (18) are hinged to the front and rear sides of the cabinet body (17), and a handle (19) is fixed to the cabinet door (18).

3. The device according to claim 2, wherein: The linkage assembly A includes a drive motor (20), a damping rotating shaft A (21), a carrier plate (28) and a guide rod (37), the drive motor (20), the damping rotating shaft A (21), the carrier plate (28) and the guide rod (37) are all fixedly installed on the top of the inner side of the cabinet body (17), the bottom of the damping rotating shaft A (21) is fixedly connected with a driven shaft A (22), the bottom end of the driven shaft A (22) is fixedly installed at the center of the tray A (1), and the outer part of the driven shaft A (22) is fixedly sleeved with a groove wheel (23), the output shaft of the drive motor (20) is connected with a transmission rod (24) through a shaft coupling, the outer part of the transmission rod (24) is fixedly sleeved with a driving dial (25) and a bevel gear A (27), a cylindrical pin (26) is fixed on the driving dial (25), and a plurality of radial grooves in a circular array are formed in the groove wheel (23).

4. The device according to claim 3, wherein: The carrier plate (28) is inserted with a driven shaft B (29), and the combination of the carrier plate (28) and the driven shaft B (29) is rotatably connected through a bearing, the tail end of the driven shaft B (29) is fixedly connected with a bevel gear B (30), the bevel gear B (30) is meshed with the bevel gear A (27), the front end of the driven shaft B (29) is fixedly connected with a rotating disc (31), the rotating disc (31) is rotatably connected with an eccentric sleeve (32) through a pin shaft, the outer part of the guide rod (37) is slidably sleeved with a fixed plate (36), the inflation assembly is installed on the bottom of the fixed plate (36), the upper surface of the fixed plate (36) is fixedly connected with a U-shaped seat (35), the U-shaped seat (35) is rotatably connected with a carrier tube (34) through a pin shaft between the opposite inner walls of the U-shaped seat (35), and the eccentric sleeve (32) and the carrier tube (34) are fixedly connected with a connecting rod (33).

5. The device according to claim 4, wherein: The inflation assembly includes a gas pump (38), the gas pump (38) is fixedly installed on the bottom of the fixed plate (36), the gas outlet of the gas pump (38) is fixedly connected with a gas delivery needle (39), the gas delivery needle (39) is used for being inserted into the penetrating hole A (9), and the outer part of the gas delivery needle (39) is sleeved with a pressing block (40).

6. The device as claimed in claim 1, wherein: The elastic lifting assembly includes a carrier cylinder (41) and a spring (42), the top of the carrier cylinder (41) and the spring (42) is fixedly installed on the lower surface of the tray A (1), the bottom end of the spring (42) is fixedly connected with a sliding rod (43), the carrier cylinder (41) is sleeved on the outer part of the sliding rod (43), and the bottom end of the sliding rod (43) is fixedly connected with a supporting plate (44).

7. The device according to claim 6, wherein: A through hole (45) required for the liquid delivery needle (10) to pass through is formed in the supporting plate (44).

8. The device according to claim 2, wherein: The linkage assembly B comprises a damping rotating shaft B (46), a driven shaft C (47) and a damping rotating shaft C (48), the upper and lower ends of the driven shaft C (47) are rotatably connected with the inner wall of the cabinet body (17) through bearings, the damping rotating shaft B (46) is fixedly installed at the top of the inner side of the cabinet body (17), the damping rotating shaft C (48) is fixedly installed at the bottom of the inner side of the cabinet body (17), the bottom of the damping rotating shaft B (46) is fixedly connected with a driven shaft D (49), the bottom end of the driven shaft D (49) is fixedly installed at the center of the tray B (2), and the outer portion of the driven shaft D (49) is fixedly sleeved with a cylindrical gear A (50), the outer portion of the driven shaft C (47) is fixedly sleeved with a cylindrical gear B (51) and a disc (52), the cylindrical gear A (50) and the cylindrical gear B (51) are mutually engaged, the top of the damping rotating shaft C (48) is fixedly connected with a supporting column (54), the top end of the supporting column (54) is fixedly installed at the center of the bottom of the tray C (3), and the outer portion of the supporting column (54) is fixedly sleeved with a cylindrical gear C (55), the circumferential side of the disc (52) is fixedly installed with a plurality of groups of tooth columns (53), the plurality of groups of tooth columns (53) are circularly arranged, and the plurality of groups of tooth columns (53) are all mutually engaged with the cylindrical gear C (55).

9. The device according to claim 8, wherein: The cylindrical gear A (50) and the cylindrical gear B (51) have the same number of teeth.

10. The device as claimed in claim 1, wherein: The bottom of the outer protection assembly is provided with a base, and the base is provided with a nitrogen blowing instrument (57), a gas chromatograph mass spectrometer (58) and a liquid chromatograph mass spectrometer (59).

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