Milk replacer for replacing calf colostrum to reduce calf diarrhea and use method

Abstract

The invention relates to the technical field of biology. The invention discloses a milk replacer capable of replacing calf colostrum and a feeding mode, and aims to reduce diarrhea of newborn calves and improve the survival rate of the newborn calves. The milk replacer contains traditional Chinese medicine extracts such as milk powder, concentrated whey protein, high-protein whey powder, cream, bovine colostrum powder, vitamin A, vitamin D3, lysozyme, saccharomyces cerevisiae boulardii, IGF, EGF growth factors, astragalus polysaccharide, chlorogenic acid and baicalin, and a feeding mode is provided. Many calves after birth cannot eat colostrum or cannot eat high-quality colostrum, the immunity of the calves is low, and the diarrhea rate is high. The product contains nutritional ingredients equivalent to calf colostrum, and through a reasonable feeding mode, passive immunity is established for newly born calves, diarrhea of the calves is reduced, the survival rate of the calves is increased, and good growth performance is obtained.

Description

I. Technical Field

[0001] This invention belongs to the field of biotechnology. The milk substitute of this invention contains extracts of traditional Chinese medicine, immunoglobulins, epidermal growth factor, and insulin-like growth factor, all of which are immune-active factors found in calf colostrum, and is fortified with Saccharomyces boulardii and other traditional Chinese medicine extracts. It enables newborn calves to acquire passive immunity, reduces the incidence of diarrhea, and improves calf growth performance. II. Background Technology

[0002] Newborn calves cannot obtain maternal antibodies through the placental barrier. Colostrum is the best way for calves to acquire maternal antibodies, establishing passive immunity after birth. However, due to limitations in production conditions or other reasons, some calves do not receive colostrum after birth, or the colostrum is of poor quality, such as having low immunoglobulin content. This leads to weakened immunity in calves, increased morbidity, and significant economic losses to the livestock industry.

[0003] Calf colostrum is yellow, highly viscous, and acidic, with high protein and fat content. The protein and fat content in colostrum is 5 times and 2 times that of regular milk, respectively. The calcium, phosphorus, sodium, and magnesium content are 1.9 times, 2.2 times, 2.1 times, and 5.5 times that of regular milk, respectively. Milk replacers for newborn calves differ significantly from conventional milk replacers.

[0004] However, most farms use conventional milk replacer. While milk replacer contains the nutrients of whole milk, it contains very little or no immunoglobulins, failing to provide calves with passive immunity. Its protein and fat content is lower than colostrum, and its lactose content is far higher, which can easily lead to diarrhea in newborn calves due to insufficient lactase. Furthermore, because milk replacer has a high water content and low dry matter content, it is not viscous enough to protect the calf's intestines. Even when bovine colostrum powder is available on the market, most focus on high immunoglobulin content without providing precise amounts of other nutrients.

[0005] Therefore, considering the current state of milk powder, we are launching a colostrum milk replacer powder, whose nutritional composition closely resembles that of colostrum. This allows newborn calves to obtain essential nutrients such as protein, fat, calcium, phosphorus, vitamins, and trace elements, as well as immune or active factors like immunoglobulins, lactoferrin, insulin-like growth factor, and epidermal growth factor, enabling them to successfully establish passive immunity. The addition of the probiotic Saccharomyces boulardii promotes the growth of beneficial gut bacteria and inhibits the proliferation of pathogenic bacteria. The addition of traditional Chinese medicine extracts such as Astragalus polysaccharide, chlorogenic acid, and baicalin enhances the calf's immunity. This improves the calf's disease resistance and survival rate, resulting in significant economic benefits. III. Summary of the Invention

[0006] Purpose of the invention Newborn calves that did not receive colostrum can establish passive immunity and improve their own immunity by consuming the milk substitute of this invention, thereby increasing their survival rate and achieving good growth performance.

[0007] Invention Idea This invention provides a milk replacer with a composition close to colostrum, enabling newborn calves to establish passive immunity. The milk replacer not only contains conventional nutrients such as protein, fat, low levels of lactose, calcium, phosphorus, and magnesium, but also immune-active factors, epidermal growth factor, insulin-like growth factor, lysozyme, and traditional Chinese medicine extracts. The comprehensive supply of nutrients and active factors in the milk replacer powder ensures the calves successfully establish passive immunity. This invention also specifies the method of using the milk replacer powder. Maintaining the appropriate temperature and proportion when mixing with water ensures the activity of the immune-active factors and the content of each component, thereby guaranteeing the effectiveness of the milk replacer powder of this invention.

[0008] Key points of the invention (1) It contains high concentrations of protein and fat and low concentrations of lactose, which meet the needs of newborn calves for conventional nutrients. (2) The high content of calcium, phosphorus and magnesium ions meets the calcium and phosphorus needs of newborn calves and promotes the excretion of meconium; (3) Rich in immunoglobulins, which are 50-150 times more abundant than normal milk, it has the functions of sterilization, anti-infection, neutralization of toxins, and enhancement of calf's own immunity. (4) The iron, zinc and manganese content is 2.8 times, 6.2 times and 1.6 times that of regular milk, respectively; (5) High in vitamins A, D, and E; (6) IGF-1 insulin-like growth factor promotes the activity of jejunal brush border enzymes, accelerates the growth and development of pancreatic and intestinal tissues, and can promote the absorption of glucose and amino acids by somatic cells. (7) EGF (epidermal growth factor) promotes the regeneration of intestinal mucosal epidermis. (8) The special dilution ratio makes the emulsion viscous and has a protective effect on the intestines. (9) Lysozyme and Saccharomyces boulardii can improve the immunity of calves. The yeast cell polysaccharide secreted by yeast promotes the absorption of nutrients by calves, inhibits bacteria and reduces inflammation. (10) The added Astragalus polysaccharide and honeysuckle extract enhance the immunity of calves, while baicalin also has antibacterial and bactericidal effects.

[0009] Technical solution of the present invention The milk substitute powder provided by this invention comprises, by weight: Milk powder 5-20g / 100g 80% whey protein concentrate 20-40g / 100g 20-45g of butter per 100g Bovine colostrum powder 10-30g / 100g Immunoglobulin 20-30g / 100g Lactoferrin 2.5-4.5g / kg (preferably 3.9g) Calcium 0.3-0.8g / 100g (0.5g preferred) Phosphorus 0.3-0.8g / 100g (preferably 0.5g) Sodium 0.10-0.18g / 100g (preferably 0.15g) Magnesium 0.10-1.15g / 100g (preferably 0.13g) Iron 6-10 mg / kg (preferably 8.1 mg / kg) Zinc 40-70 mg / kg (preferably 62 mg / kg) Manganese 200-400 mg / kg (preferably 300 mg / kg) Vitamin A 70-100 mg / kg (preferably 95 mg / kg) Vitamin D 15-30 mg / kg (preferably 22 mg / kg) Vitamin E 60-100mg / kg (preferably 80mg / kg) EGF 5-9ug / kg (preferably 7ug / kg) IGF 200-900ug / kg (preferably 800ug / kg) Lysozyme is preferably administered at a rate of 0.5-2 g / kg (preferably 1 g / kg). The optimal dosage of *Bladiomycin* is 1-2.5 g / kg (2 g / kg). A complex of Astragalus polysaccharide, honeysuckle chlorogenic acid, and baicalin extracts at 0.5-3 g / kg (preferably 1 g / kg).

[0010] Effects of the present invention The effects of the invention: First, its high content of nutrients such as protein, fat, calcium, phosphorus, and vitamins can promote the growth and development of newborn calves. Secondly, a sufficient supply of immunoglobulins enables newborn calves to acquire immune-active factors. Third, a moderate amount of magnesium ions can promote the expulsion of meconium, stimulate the secretion of digestive juices in the gastrointestinal tract, and inhibit the proliferation of harmful bacteria. Fourth, the gastrointestinal mucosa of newborn calves is not well developed, and the abomasum is not fully developed. The viscous solution after dilution with water can form a protective film to protect the calf's gastrointestinal tract. Fifth, butter is pure animal fat, which is easily digested and absorbed by calves, reducing diarrhea. Sixth, the high content of IGF and EGF promotes the absorption of glucose and amino acids and the regeneration of intestinal mucosal epithelium. Sixth, lactoferrin has a resistance effect against common bacteria such as Escherichia coli and Staphylococcus aureus. Seventh, lysozyme can kill bacteria while also promoting the growth of bifidobacteria, which is beneficial for the establishment of rumen microorganisms and normal gastrointestinal flora. Eighth, Saccharomyces boulardii binds to pathogens, preventing them from adhering to the intestinal epithelium and reducing the incidence of diarrhea. Ninth, Astragalus polysaccharides increase the activity of antioxidant enzymes in calf blood, enhance calf immunity, and have inhibitory effects on Streptococcus and Staphylococcus. Honeysuckle chlorogenic acid has broad-spectrum antibacterial and antiviral effects, and enhances calf immunity. Baicalin has heat-clearing, sedative, probiotic, and anti-inflammatory effects. The use of this generation of dairy products ensures the survival rate and growth performance of newborn calves, and improves production efficiency. IV. Detailed Embodiments of the Invention

[0011] Example 1 This invention relates to a milk replacer for reducing calf diarrhea by replacing calf colostrum, comprising the following ingredients by weight percentage: The aforementioned dairy product comprises 1000 parts by weight, of which 100 parts by weight of milk powder WPC80 Whey Protein Concentrate 300 servings 300 servings of butter 200 parts by weight of bovine colostrum powder 100 parts by weight of intermediate.

[0012] The 100 parts by weight of the intermediate comprises: Calcium lactate 30-60 parts by weight (preferably 35 parts) 15-40 parts by weight of calcium dihydrogen phosphate (preferably 25 parts) Sodium chloride 2.5-4.6 parts by weight (preferably 3.8 parts by weight) 1.7-2.5 parts by weight of magnesium oxide (preferably 2.2 parts by weight) Ferrous glycine is preferred at 470 mg / kg The preferred zinc glycine concentration is 3100 mg / kg. Manganese glycine is preferably 1500 mg / kg Vitamin A is preferred at 950 mg / kg Vitamin D 22mg / kg is preferred Vitamin E 800mg / kg is preferred EGF 5-9ug / kg (preferably 7ug / kg) IGF 200-900ug / kg (preferred 800ug / kg) Lactoferrin 2.5-4.5 parts by weight (preferably 3.9 parts by weight) Lysozyme is preferably 1g / kg, and Saccharomyces boulardii is preferably 2g / kg. A complex of Astragalus polysaccharide, Lonicera japonica chlorogenic acid, and baicalin extracts is 1g / kg. The carrier is glucose.

[0013] How to use: (1) Preparation of trace component compound: Iron, zinc, manganese, vitamin A, vitamin B, vitamin E, EGF, IGF, lactoferrin, lysozyme, Saccharomyces boulardii, Astragalus polysaccharide, honeysuckle chlorogenic acid, and baicalin extract of traditional Chinese medicine are mixed evenly according to the ratio to obtain trace component compound. (2) Mix calcium lactate, calcium dihydrogen phosphate, sodium chloride and magnesium oxide evenly according to the ratio, and add trace component mixing agent to the mixture to obtain intermediate. (3) Mix the milk powder, WPC80 protein concentrate, bovine colostrum powder and intermediate evenly according to the formula. (4) Take 70 parts by weight of the mixture obtained in (3) and add 300 parts by weight of warm water at 40-50℃ to dissolve it. (5) Dissolve 30 parts by weight of butter at 40-45℃ and add it to the solution in (4). When it is cooled to 38-39℃, it can be fed. This embodiment is mainly applicable to calves with a birth weight of 40-50 kg.

[0014] Example 2 It is basically the same as Example 1, except that The aforementioned dairy product comprises 1000 parts by weight, of which 200 parts by weight of milk powder WPC80 whey protein concentrate 250 servings 250 parts by weight of butter 200 parts by weight of bovine colostrum powder This embodiment is mainly applicable to calves with a birth weight of less than 40 kg.

[0015] Example 3 It is basically the same as Example 1, except that The aforementioned dairy product comprises 1000 parts by weight, of which 100 parts by weight of milk powder WPC80 whey protein concentrate 350 servings 250 parts by weight of butter 200 parts by weight of bovine colostrum powder This embodiment is mainly applicable to calves with a birth weight of more than 50 kg.

[0016] The milk replacers from Examples 1 and 2 were used to feed newborn calves for testing. The specific details of the experiment are as follows: Specific experimental details

[0017] Thirty-two healthy newborn calves of similar birth weight and age were selected and randomly divided into two groups of 16 calves each using a one-way completely randomized experimental design. Analysis of variance showed no significant difference in initial weight between the two groups. The control group calves were fed colostrum produced by their mothers, while the experimental group calves were fed colostrum-based milk replacer.

[0018] Feeding method: Mix colostrum milk replacer with warm water at a ratio of 1:3, and then pasteurize. Feed 4 liters within half an hour of birth, and another 2 liters within 6 hours, with the total amount being 10% of the baby's body weight. The study examined serum IgG, average daily weight gain, morbidity, diarrhea rate, and mortality rate in calves. Experiment 1 used the milk substitute from Example 1. The experimental results are shown in the table below. The effect of colostrum milk replacer on the establishment of passive immunity and growth performance in newborn calves

[0019] Experiment 2 used the milk substitute from Example 2. The experimental results are shown in the table below. The effect of colostrum milk replacer on the establishment of passive immunity and growth performance in newborn calves

Claims

1. A milk substitute for reducing calf diarrhea by replacing calf colostrum and its method of use, characterized in that, Based on parts by weight: Milk powder 5-20g / 100g 80% whey protein concentrate 20-40g / 100g 20-45g of butter per 100g Bovine colostrum powder 10-30g / 100g Calcium 0.3-0.8g / 100g (0.5g preferred) Phosphorus 0.3-0.8g / 100g (preferably 0.5g) Sodium 0.10-0.18g / 100g (preferably 0.15g) Magnesium 0.10-1.15g / 100g (preferably 0.13g) Iron 6-10 mg / kg (preferably 8.1 mg / kg) Zinc 40-70 mg / kg (preferably 62 mg / kg) Manganese 200-400 mg / kg (preferably 300 mg / kg) Vitamin A 70-100 mg / kg (preferably 95 mg / kg) Vitamin D 15-30 mg / kg (preferably 22 mg / kg) Vitamin E 60-100mg / kg (preferably 80mg / kg) Lactoferrin 2.5-4.5g / kg (preferably 3.9g) EGF 5-9ug / kg (preferably 7ug / kg) IGF 200-900ug / kg (preferred 800ug / kg) Lysozyme is preferably administered at a rate of 0.5-2 g / kg (preferably 1 g / kg). The optimal dosage of *Bladiomycin* is 1-2.5 g / kg (2 g / kg). A complex of Astragalus polysaccharide, honeysuckle chlorogenic acid, and baicalin extracts at 0.5-3 g / kg (preferably 1 g / kg). The method of using this dairy product is to dilute it with water at a ratio of 1:3, melt the butter beforehand, and then mix it evenly with the other ingredients.

2. The milk substitute for reducing calf diarrhea as described in claim 1, and its method of use, are characterized in that, The raw material components include the following parts by weight: The aforementioned dairy product comprises 1000 parts by weight, of which 100 parts by weight of milk powder WPC80 Whey Protein Concentrate 300 servings 300 servings of butter 200 parts by weight of bovine colostrum powder 100 parts by weight of intermediate.

3. The milk substitute for reducing calf diarrhea as described in claim 1, and its method of use, are characterized in that... The raw material components include the following parts by weight: The aforementioned dairy product comprises 1000 parts by weight, of which 200 parts by weight of milk powder WPC80 whey protein concentrate 250 servings 250 parts by weight of butter 200 parts by weight of bovine colostrum powder 100 parts by weight of intermediate.

4. The milk substitute for reducing calf diarrhea as described in claim 1, and its method of use, characterized in that, The raw material components include the following parts by weight: The aforementioned dairy product comprises 1000 parts by weight, of which 100 parts by weight of milk powder WPC80 whey protein concentrate 350 servings 250 parts by weight of butter 200 parts by weight of bovine colostrum powder 100 parts by weight of intermediate.

5. The milk substitute for reducing calf diarrhea as described in claims 2, 3, and 4, and its method of use, characterized in that, 100 parts by weight of the intermediate comprises the following raw material components: The 100 parts by weight of the intermediate comprises: Calcium lactate 30-60 parts by weight (preferably 35 parts) 15-40 parts by weight of calcium dihydrogen phosphate (preferably 25 parts) Sodium chloride 2.5-4.6 parts by weight (preferably 3.8 parts by weight) 1.7-2.5 parts by weight of magnesium oxide (preferably 2.2 parts by weight) Ferrous glycine is preferred at 470 mg / kg The preferred zinc glycine concentration is 3100 mg / kg. Manganese glycine is preferably 1500 mg / kg Vitamin A is preferred at 950 mg / kg Vitamin D 22mg / kg is preferred Vitamin E 800mg / kg is preferred EGF 5-9ug / kg (preferably 7ug / kg) IGF 200-900ug / kg (preferred 800ug / kg) Lactoferrin 2.5-4.5 parts by weight (preferably 3.9 parts by weight) Lysozyme is preferably 1g / kg, and Saccharomyces boulardii is preferably 2g / kg. A complex of Astragalus polysaccharide, honeysuckle chlorogenic acid, and baicalin extracts is 1g / kg. The carrier is glucose.

6. The milk substitute for reducing calf diarrhea as described in claim 1, and its method of use, characterized in that, Preparation and usage of milk substitutes: (1) Preparation of trace component compound: Iron, zinc, manganese, vitamin A, vitamin B, vitamin E, EGF, IGF, lactoferrin, lysozyme, Saccharomyces boulardii, Astragalus polysaccharide, honeysuckle chlorogenic acid, and baicalin extract of traditional Chinese medicine are mixed evenly according to the ratio to obtain trace component compound. (2) Mix calcium lactate, calcium dihydrogen phosphate, sodium chloride and magnesium oxide evenly according to the ratio, and add trace component mixing agent to the mixture to obtain intermediate. (3) Mix the milk powder, WPC80 protein concentrate, bovine colostrum powder and intermediate evenly according to the formula. (4) Take 70 parts by weight of the mixture obtained in (3) and add 300 parts by weight of warm water at 40-50℃ to dissolve it. (5) Dissolve 30 parts by weight of butter at 40-45℃ and add it to the solution in (4). When it is cooled to 38-39℃, it can be fed.

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