Food composition, pharmaceutical composition, quasi-pharmaceutical composition, feed composition and preparation method thereof

Abstract

The invention relates to a food composition, a pharmaceutical composition, a quasi-pharmaceutical composition, a feed composition and a preparation method thereof. The present invention relates to a food composition containing fermented and aged morinda citrifolia or an extract thereof and a dried kammann orange extract in a weight ratio of 90: 10 as active ingredients, which reduces the secretion of cortisol, which is a stress hormone, and increases the secretion of serotonin, which is a happy hormone, and which has no side effects, and can be used as a food composition for the production of food products. The human body is safe. The composition disclosed by the invention can be used as a health food or a pharmaceutical composition. The composition provided by the invention has an excellent pressure resistance effect.

Description

[0001] This application is a divisional application of the Chinese Patent Application No. 202111632200.5, filed on December 28, 2021, with the title of “Food composition, pharmaceutical composition, quasi-drug composition, feed composition, and preparation method thereof”. TECHNICAL FIELD

[0002] The present application relates to a composition comprising fermented aged Morinda officinalis and Citrus kamanu as an active ingredient, and more particularly, to a composition comprising fermented aged Morinda officinalis and Citrus kamanu as an active ingredient, which relieves stress and prevents and improves symptoms caused by stress. BACKGROUND

[0003] Stress, which is called the source of all diseases, exists in all areas of life of humans or animals. Stress is a strong stimulus induced by various factors from a small matter to a major event in daily life, and induces fatigue and boredom, depression, and the like, and causes internal and external negative reactions.

[0004] When a stimulus to the body is generally above a certain degree, it causes damage to the body, and at this time, the organism exhibits a non-specific general adaptive syndrome in response to a certain threat regardless of the type of the stimulus, and this phenomenon is called stress (Selye, H.: The stress of life, Toronto, Longmans Green and Co., pp. 1-50, 1958). There are various types of stress, and as examples thereof, there are tension headache, migraine, hypertension, indigestion, fatigue, pain, menopausal disorder, hair loss, and rough skin. Also, if stress is chronically sustained, various neuroses, gastric ulcers, and the like are exhibited, and the physiological activity, immune activity, and the like of the body are reduced, mental activity is suppressed, and emotional instability is caused, and abnormal behavior is induced.

[0005] In particular, since stress is excessively generated in modern society, stress is highlighted as a very important social problem.

[0006] The causes of stress and its physical changes are diverse, but they commonly result in hyperfunction of the brain-pituitary-adrenal system (HPA) as the sympathetic nervous system, inducing hormone secretion, resulting in hyperfunction of the adrenal gland and hypofunction of the spleen, the result of which is known to cause all symptoms due to stress. If stress is increased, the spleen is significantly reduced in weight due to the reduction in immune function, the adrenal gland is hyperfunctioned by adrenocorticotropic hormone, the weight is increased, the phenomenon of lipid reduction occurs, and the secretion of cortisol is promoted. The amount of cortisol is in the tendency to increase according to age and stress, which in turn contributes to obesity. Also, if stress is increased, the adrenal gland secretes hormones, increasing cholesterol, blood sugar, lactate dehydrogenase (LDH), etc. in the blood.

[0007] On the other hand, for gastric ulcers related to the depth of stress, it is known that reactive oxygen species are associated with the generation of ulcers of such a digestive organ. The biological defense system for removing reactive oxygen or reactive oxygen species (ROS) is generally classified into an enzyme defense system based on enzyme systems such as superoxide dismutase (SOD), catalase, glutathione peroxidase, and a non-enzyme defense system that interrupts or ends the chain reaction of ROS or free radicals. Living organisms that utilize oxygen have an enzyme that removes superoxide, i.e., SOD, to protect the organism from damage caused by superoxide. Catalase removes H2O2 generated in tissues by enzyme reactions such as SOD to defend the function of the organism. Also, it has been reported that not only free radicals that remove reactive oxygen species generated during the metabolic process, but also these reactive oxygen species can irreversibly inactivate free radicals generated by lipid oxidation. Glutathione (GSH), which occupies a large portion of non-protein thiols in animal tissues, functions as a free radical remover, and as a substrate for GSH-Px that neutralizes H2O2 and peroxidized lipids, it plays an important role as an intracellular antioxidant (Cho SY. et al., J. Korean Soc. Food Sci. Nutr., 32(3), pp458-463, 2003).

[0008] Recently, various methods have been sought to relieve stress, the most effective of which utilizes exercise or psychotherapy, etc. Also, there is a method of minimizing the damage caused by stress by parallel drug therapy according to the intensity of stress. In particular, as a drug for relieving stress, psychotropic drugs such as benzodiazepines as neuroleptics, tranquilizers as antipsychotics, etc. are used.

[0009] However, in order to release stress, there is a risk of poisoning when using the above stabilizer, and there is a problem in that it is difficult to eliminate side effects in the properties of synthetic drugs. There is a negative effect in that over-reliance on drugs causes another kind of stress.

[0010] Recently, a number of foods for fatigue recovery or stress release using Chinese herbal medicine materials have been developed.

[0011] Among the related researches on compositions for fatigue recovery using natural Chinese medicine materials, a functional beverage using an extract of Ligusticum chuanxiong, Glycyrrhiza uralensis, Pueraria lobata, Hovenia dulcis, Alnus hirsuta, Citrus aurantium, Zizyphus jujuba, Oenanthe javanica, and Ginkgo biloba leaves is disclosed in Korean Laid-open Patent Publication No. 2000-0066777, and a fatigue recovery medicinal beverage containing Rhizoma Polygonati, Membranae Rehmanniae, Fructus Lycii, and Fructus Schisandrae is disclosed in Korean Laid-open Patent Publication No. 10-2005-0108856.

[0012] Among the related researches on foods for stress release in Korea, a health food for stress release using deep water, organic germanium, and Rhizoma Cyperi, Citrus aurantium, malt, Semen Ziziphi Spinosae, and Zizyphus jujuba as essential ingredients is disclosed in Korean Laid-open Patent Publication No. 10-2004-0006705, and a stress release food using red ginseng, Gastrodia elata, and Rehmannia glutinosa juice as main raw materials is disclosed in Korean Laid-open Patent Publication No. 10-2004-0018888. Also, a flavored Sijunzi decoction with an anti-stress effect by adding Prunus mume, Polygonatum odoratum, Pueraria lobata, and Cinnamomum cassia extract to the conventional Sijunzi decoction is disclosed in Korean Laid-open Patent Publication No. 10-2005-0080455.

[0013] It is known that a large number of plants existing in nature contain functional ingredients having biological regulation functions such as disease prevention and aging inhibition, and research on natural food raw materials is actively being conducted.

[0014] Therefore, research on developing functional supplements using natural substances with guaranteed safety like plant extracts is actively being conducted. As a result of the inventors' continuous research on fermented aged Morinda citrifolia and Citrus kamanu, it was confirmed that the intake of these complexes is effective for stress release, and thus the present invention was completed.

[0015] Prior Art Documents

[0016] Patent Documents

[0017] (Patent Document 1) Korean Laid-open Patent Publication No. 2000-0066777

[0018] (Patent Document 2) Korean Laid-open Patent Publication No. 10-2005-0108856 SUMMARY

[0019] The present application has been made to solve the aforementioned problems and other problems that are related thereto.

[0020] The present application has been made to solve the aforementioned problems and other problems that are related thereto.

[0021] An object of the present application is to provide a stress relieving composition comprising fermented aged Morinda citrifolia or extract thereof and extract of Citrus kamanu as an active ingredient.

[0022] Another object of the present application is to provide a method for preparing a composition comprising fermented aged Morinda citrifolia or extract thereof and extract of Citrus kamanu as an active ingredient.

[0023] Another object of the present application is to provide a stress relieving food composition comprising fermented aged Morinda citrifolia or extract thereof and extract of Citrus kamanu as an active ingredient.

[0024] Another object of the present application is to provide a stress relieving pharmaceutical composition comprising fermented aged Morinda citrifolia or extract thereof and extract of Citrus kamanu as an active ingredient.

[0025] Another object of the present application is to provide a stress relieving quasi-pharmaceutical composition comprising fermented aged Morinda citrifolia or extract thereof and extract of Citrus kamanu as an active ingredient.

[0026] Another object of the present application is to provide a stress relieving feed composition comprising fermented aged Morinda citrifolia or extract thereof and extract of Citrus kamanu as an active ingredient.

[0027] The technical problems to be solved according to the inventive concept disclosed in the present specification are not limited to the technical problems for solving the above-mentioned problems, and another technical problem not mentioned can be clearly understood by those skilled in the art of the present application through the following description.

[0028] Specifically, each of the explanations and embodiments disclosed in the present application can be applied to each of the other explanations and embodiments. That is, all combinations of the various factors disclosed in the present application are within the scope of the present application. Furthermore, the scope of the present application should not be construed as being limited to the specific explanations described below.

[0029] To solve the above problems, the present application provides a stress relieving or prevention or treatment of stress disease composition comprising fermented aged Morinda citrifolia or extract thereof and extract of Citrus kamanu as an active ingredient.

[0030] Hereinafter, the present application will be described in detail.

[0031] In the present invention, "fermented and aged Noni" refers to Noni fermented and aged by inoculating lactic acid bacteria in Noni. Herein, Noni is not limited by its part, but Noni fruit can be used as an example.

[0032] "Fermented Noni" can be a fermentation product of Noni fruit inoculated with any one or more of the following seven lactic acid bacteria:

[0033] (1) Lactobacillus plantarum;

[0034] (2) Lactobacillus paracasei;

[0035] (3) Lactobacillus rhamnosus;

[0036] (4) Lactobacillus casei;

[0037] (5) Lactobacillus fermentum;

[0038] (6) Lactobacillus reuteri; and

[0039] (7) Lactococcus lactis subsp. lactis.

[0040] The above "fermented Noni" is fermented using lactic acid bacteria having excellent bioconversion ability, and can bioconvert Scopolin and Asperuloside, which are glycoside compounds contained in Noni, into scopoletin, deacetylasperulosidi C acid (DAA), and asperulosidi C acid, which are non-glycoside compounds.

[0041] The content of bioconverted scopoletin contained in the extract of the above "fermented Noni" is 1 to 200 μg / mL, the content of deacetylasperulosidi C acid is 0.2 to 0.6 mg / mL, and the content of asperulosidi C acid is 0.096 to 1.41 mg / mL. Within the error of the analysis range, the fermented and aged Noni also contains the contents of scopoletin, deacetylasperulosidi C acid, and asperulosidi C acid contained in the fermented Noni.

[0042] The "fermented and aged Noni" of the present invention can be fermented and aged Noni itself or an extract therefrom.

[0043] As an example, the fermentation-aged Noni of the present application can be Noni fruit inoculated with lactic acid bacteria and fermented and aged, and an extract of a liquid obtained by juicing these fermentation-aged Noni.

[0044] Calamansi is a tropical fruit, which is a fruit cultivated in Southeast Asia. The Calamansi extract refers to juice extracted from the Calamansi, and the extraction method of the juice is a matter well known in the art, and thus a detailed description thereof is omitted. Also, instead of preparing the extract additionally, a Calamansi raw liquid commercially available can be used. The Calamansi contains more than 30 times the amount of vitamin C than lemon, and contains a large amount of cryptoxanthin, hesperidin, nobiletin, cinehulin, etc.

[0045] In the present specification, "extract" means a crude extract commonly used in the art, but in a broad sense, also includes a fraction obtained by further fractionating the extract. That is, the fermentation-aged Noni or extract thereof and the Calamansi extract include not only an extract obtained using the extraction solvent, but also an extract obtained by additionally applying a refining process thereto. For example, a fraction obtained by passing the extract through an ultrafiltration membrane having a predetermined molecular weight cut off value, a fraction obtained by various purification methods additionally applied such as separation based on various chromatography (size, charge, and separation based on hydrophobicity or affinity), etc. are also included in the fermentation-aged Noni and Calamansi extract of the present application.

[0046] In the present specification, "comprising as an effective ingredient" means an amount sufficient to achieve the efficacy or activity of the fermentation-aged Noni or extract thereof and the Calamansi extract. The present application is a composition extracted from the fermentation-aged Noni and Calamansi, which are natural plant materials, and has no side effects on the human body even if administered in excess, and thus the upper limit of the amount of the fermentation-aged Noni and Calamansi extract contained in the composition of the present application can be selected within an appropriate range by a person of ordinary skill in the art.

[0047] The fermentation-aged Noni and Calamansi extract according to the present application are extracted using an organic solvent, and the solvent that can be used as the extraction solvent is as follows.

[0048] First, as the polar solvent, there are (i) water, (ii) alcohols having 1 to 6 carbon atoms (preferably, methanol, ethanol, propanol, butanol, n-propanol, isopropanol, n-butanol, 1-pentanol, 2-butoxyethanol, or ethylene glycol), (iii) acetic acid, (iv) dimethylformamide (DMFO), and (v) dimethyl sulfoxide (DMSO), and the like.

[0049] Further, as the non-polar solvent, there are acetone, acetonitrile, ethyl acetate, methyl acetate, fluoroalkane, pentane, hexane, 2,2,4-trimethylpentane, decane, cyclohexane, cyclopentane, diisobutylene, 1-pentene, 1-chlorobutane, 1-chloropentane, o-xylene, diisopropyl ether, 2-chloropropane, toluene, 1-chloropropane, chlorobenzene, benzene, diethyl ether, diethyl sulfide, chloroform, dichloromethane, 1,2-dichloroethane, aniline, diethylamine, ether, carbon tetrachloride, dichloromethane, petroleum ether, and THF, and the like.

[0050] As a representative example of the extraction solvent of the present application, (a) water, (b) anhydrous or lower alcohols having 1 to 4 carbon atoms (methanol, ethanol, propanol, butanol, and the like), (c) a mixed solvent of the above lower alcohols and water, (d) acetone, (e) ethyl acetate, (f) chloroform, (g) butyl acetate, (h) 1,3-butanediol, (i) hexane, and (j) diethyl ether, and the like are preferably used, and for easy extraction, water, ethanol, or a mixture of water and ethanol can be used for extraction.

[0051] Further, the fermentation-aged Morinda citrifolia or extract thereof and the extract of Citrus kamanutensis used in the present application can be prepared in a powder state by an additional process such as reduced pressure distillation and freeze-drying or spray-drying, and the like.

[0052] In the present application, it is characterized that the weight ratio of the above fermentation-aged Morinda citrifolia or extract thereof and the extract of Citrus kamanutensis is 70:30 to 99:1, or 80:20 to 95:5, or 70:30 to 90:10.

[0053] According to an embodiment of the present application, the present application relates to a food composition for pressure relief containing fermentation-aged Morinda citrifolia or extract thereof and extract of Citrus kamanutensis as an effective ingredient.

[0054] The above food composition can include, for example, a health food composition, and the health food composition can include various food, beverages, chewing gum, tea, vitamin complexes, health foods, and the like, and can be used in the form of powder, granules, tablets, capsules, or beverages. The food composition of each dosage form can be appropriately selected from ingredients commonly used in the relevant field, in addition to the effective ingredient, according to the dosage form or the purpose of use, and can produce an enhancing effect when used together with other ingredients.

[0055] The above composition can include other ingredients that can enhance the main effect, within a range that does not impair the main effect of the present application. For example, in order to improve physical properties, additives such as flavorings, pigments, bactericides, antioxidants, preservatives, humectants, tackifiers, inorganic salts, emulsifiers, and synthetic high molecular substances can also be included.

[0056] When the health food composition of the present application is used as a food additive, the above fermented and aged Morinda citrifolia, Citrus kamanut can be added as is, or can be used together with other foods or food ingredients, and can be appropriately used according to conventional methods. The mixing amount of the effective ingredient can be appropriately determined according to the purpose of use (prevention, health, or therapeutic treatment). The type of the above food is not particularly limited. As examples of foods to which the above fermented and aged Morinda citrifolia, Citrus kamanut can be added, there are meat, sausage, bread, chocolate, candy, fast food, cookies, pizza, instant noodles, other noodles, chewing gum, dairy products including ice cream, various soups, beverage water, tea, rehydration solutions, alcoholic beverages, vitamin complexes, and the like, all of which include health foods in the conventional sense.

[0057] When the composition of the present application is used as a health beverage, various flavorings or natural carbohydrates, and the like can be included as additional ingredients, as in conventional beverages. The above natural carbohydrates are monosaccharides such as glucose and fructose; disaccharides such as maltose and sucrose; polysaccharides such as dextrin and cyclodextrin; sugar alcohols such as xylitol, sorbitol, and erythritol. As a sweetener, a natural sweetener such as thaumatin or stevioside extract, or a synthetic sweetener such as saccharin or aspartame, or the like can be used.

[0058] The present application provides a method of preparing a composition including fermented and aged Morinda citrifolia and Citrus kamanut, which includes the steps of obtaining fermented and aged Morinda citrifolia fermented and aged by lactic acid bacteria, and additionally mixing Citrus kamanut in the above fermented and aged Morinda citrifolia.

[0059] According to an embodiment of the present application, the present application provides a method of preparing a composition including fermented and aged Morinda citrifolia or an extract thereof and a Citrus kamanut extract, which includes the steps of (a) obtaining fermented and aged Morinda citrifolia fermented and aged by lactic acid bacteria, and (b) additionally aging Citrus kamanut in the above fermented and aged Morinda citrifolia.

[0060] According to an embodiment of the present application, the present application provides a method for preparing a composition for stress relief, characterized by comprising: step (a) inoculating lactic acid bacteria into a Morinda citrifolia fruit to prepare a fermented Morinda citrifolia which is biotransformed by fermentation and aging; step (b) juicing the fermented and aged Morinda citrifolia; and step (c) adding and mixing a Citrus kawamii to the fermented and aged Morinda citrifolia.

[0061] The fermentation and aging can be performed at 35 to 40°C for 48 to 168 hours.

[0062] The aging can be performed by spraying the fermentation broth generated during the fermentation at regular time intervals to the upper portion of the fermented material.

[0063] The biotransformed fermented Morinda citrifolia can be a fermentation product of the Morinda citrifolia inoculated with one or more of the above-described seven kinds of lactic acid bacteria.

[0064] According to an embodiment of the present application, the present application relates to a pharmaceutical composition for stress relief or prevention or treatment of stress diseases, which comprises a fermented and aged Morinda citrifolia or an extract thereof and a Citrus kawamii extract as an active ingredient.

[0065] In the present application, the secretion of cortisol, which is a stress hormone, is confirmed to be inhibited, thereby also inhibiting behavioral changes caused by stress, and thus it is known that the effects of prevention or treatment of stress diseases such as depression, anxiety, fatigue syndrome, sleep disorder, panic disorder, memory loss, weakness, insomnia, Parkinson's disease, and Alzheimer's disease are exhibited.

[0066] Accordingly, the present application relates to a pharmaceutical composition for prevention or treatment of stress diseases, which comprises a fermented and aged Morinda citrifolia or an extract thereof and a Citrus kawamii extract as an active ingredient.

[0067] The pharmaceutical composition according to the present application comprises the active ingredient alone or together in a suitable form of a dosage form with a pharmaceutically acceptable carrier, and can further comprise an excipient or a diluent. The above-described "pharmaceutically acceptable" means a non-toxic composition that is physiologically acceptable and administered to the human body, and generally refers to a composition that does not cause allergic reactions such as gastrointestinal disorders, dizziness, or the like, or reactions similar thereto.

[0068] As the pharmaceutically acceptable carrier, for example, an oral carrier or a non-oral carrier can be further added. The oral carrier can include lactose, starch cellulose derivatives, magnesium stearate, stearic acid, and the like. Also, various drug delivery substances for the use of the peptide preparation as oral can be included. Also, the non-oral carrier can include water, suitable oil, physiological saline, aqueous glucose, and ethylene glycol, and the like, and a stabilizer and a preservative can be further added. As the suitable stabilizer, there are antioxidants such as sodium bisulfite, sodium sulfite, or ascorbic acid. Suitable preservatives are benzalkonium chloride, methylparaben, or propylparaben, and chlorobutanol. The pharmaceutical composition of the present application can further include a lubricant, a humectant, a sweetener, a flavoring agent, an emulsifier, a suspending agent, and the like, in addition to the above-mentioned ingredients. In addition, the pharmaceutically acceptable carrier and the preparation can be referred to the contents described in the following document (Remington's Pharmaceutical Sciences, 19th ed., Mack Publishing Company, Easton, PA, 1995).

[0069] The composition of the present application can be administered to mammals including humans by any method. For example, it can be administered orally or non-orally. The non-oral administration method is not limited thereto, but can be intravenous, intramuscular, intra-arterial, intramedullary, intradural, intracardiac, transdermal, subcutaneous, intraperitoneal, intranasal, intestinal, topical, or sublingual or intrarectal administration.

[0070] The pharmaceutical composition of the present application can be formulated as an oral or non-oral preparation according to the administration route as described above.

[0071] As for the oral preparation, the composition of the present application can be formulated as a powder, granule, tablet, pill, sugar-coated tablet, capsule, liquid, gel, syrup, slurry, suspension, and the like, using a method well known in the art. For example, the oral preparation, after the active ingredient is mixed with a solid excipient and then pulverized, and after a suitable auxiliary agent is added, a granular mixture is processed, whereby a tablet or sugar-coated tablet can be obtained. As an example of the suitable excipient, there can be included a sugar such as lactose, glucose, sucrose, sorbitol, mannitol, xylitol, erythritol, and maltitol, a starch such as corn starch, wheat starch, rice starch, and potato starch, a cellulose such as cellulose, methyl cellulose, sodium carboxymethyl cellulose, and hydroxypropyl methyl cellulose, a filler such as gelatin, polyvinylpyrrolidone, and the like. Also, according to circumstances, a cross-linked polyvinylpyrrolidone, agar, alginic acid, or sodium alginate, and the like as a disintegrating agent can be added. Further, the pharmaceutical composition of the present application can further include an anti-aggregation agent, a lubricant, a humectant, a flavoring agent, an emulsifier, a preservative, and the like.

[0072] For non-oral preparations, the formulation can be in the form of an injection, a cream, an emulsion, an external ointment, an oil, a moisturizer, a gel, an aerosol, and a nasal inhalant by methods known in the art. These formulations are described in the prescription literature generally known in all pharmaceutical chemistry (Remington's Pharmaceutical Science, 19th ed., Mack Publishing Company, Easton, PA, 1995).

[0073] The total effective amount of the composition of the present application can be administered to a patient in a single dose, and can be administered by a fractionated treatment protocol in which multiple doses are administered over a long period of time. The pharmaceutical composition of the present application can vary the content of the effective ingredient according to the degree of the disease. Preferably, for the preferred total volume of the pharmaceutical composition of the present application, it can be about 0.01 μg to 10,000 mg, preferably 0.1 μg to 500 mg, and most preferably 100 mg to 500 mg per 1 kg of the patient's body weight per day. However, the volume of the above pharmaceutical composition is determined in relation to the effective dose of the patient considering not only the formulation method, the administration route, and the number of treatments, but also various factors such as the age, the body weight, the health status, the sex, the severity of the disease, the diet, and the excretion rate of the patient, and thus the appropriate effective dose of the composition of the present application can be determined by those skilled in the art when this matter is considered. The pharmaceutical composition according to the present application is not particularly limited in the dosage form, the administration route, and the administration method as long as the effects of the present application are exhibited.

[0074] According to an embodiment of the present application, the present application relates to a quasi-drug composition for the prevention or treatment of stress or stress-related diseases, comprising fermented Morinda citrifolia or an extract thereof and a Camellia sinensis extract.

[0075] The term "quasi-drug" used in the present application means an article used for the purpose of diagnosis, treatment, alleviation, or prevention of diseases in humans or animals, excluding an article that is not a device, machine, or apparatus, and an article used for the purpose of bringing about a pharmacological effect on the structure and function of humans or animals, excluding an article that is not a device, machine, or apparatus, and as a specific example, can include an internal preparation, but is not limited thereto, and the formulation method, volume, method of use, structural components, etc. of the quasi-drug can be appropriately selected from conventional techniques known in the art.

[0076] The quasi-pharmaceutical composition of the present application can further include a pharmaceutically acceptable carrier, excipient or diluent, as necessary, in addition to the above-mentioned components. The above-mentioned pharmaceutically acceptable carrier, excipient or diluent is not limited as long as it does not impair the effects of the present application, and for example, can include a filler, extender, binding agent, wetting agent, disintegrating agent, surfactant, lubricant, sweetening agent, flavoring agent, preservative, etc.

[0077] According to an embodiment of the present application, the present application relates to a feed composition for stress relief or prevention or treatment of stress disease, comprising fermented aged Morinda officinalis or extract thereof and extract of Citrus kaman.

[0078] In the present application, the above-mentioned feed composition can be formulated in the form of a conventional feed, and can include known feed components together. Also, a feed additive added in the form of an additive to the used feed can be used. The feed additive of the present application corresponds to a supplementary feed under the Feed Management Act, and can further include sodium bicarbonate (sodium bicarbonate), bentonite, magnesium oxide, a mineral preparation such as a complex mineral, a mineral preparation of a trace mineral such as zinc, copper, cobalt, selenium, a vitamin preparation such as carotene, vitamin E, vitamin A, D, E, niacin, vitamin B complex, an amino acid protective agent such as methionine, lysine, a fatty acid protective agent such as calcium salt of fatty acid, a probiotic such as lactic acid bacteria preparation, yeast culture, mold fermentation, etc.

[0079] The feed or feed additive of the present application can be applied to various animal feeds including mammals, poultry and fish.

[0080] The fermented aged Morinda officinalis or extract thereof and extract of Citrus kaman included in the composition for stress relief according to the present application have an effect of relieving stress using a natural medicinal material and food composition, and preventing symptoms (disease) caused by stress or effectively improving them without side effects.

[0081] Also, the fermented aged Morinda officinalis secretes components (scopoletin, deacetyl scillarenin and scillarenin) that improve bioconversion of digestive absorption in the fermentation process of Morinda officinalis fruit, thereby having an effect of promoting digestion and defecation activity of adults and elderly people who have relatively poor digestion, and on the other hand, since it is a natural raw material, it has no toxicity and side effects, so it can be safely ingested.

[0082] Also, since the preparation process of the fermented aged Morinda officinalis is simple, the preparation cost can be greatly reduced, thereby having an effect of greatly reducing the purchase cost burden of consumers.

[0083] Also, the fermented aged Morinda officinalis and Citrus kaman complex composition is a food composition in which the preference function is improved compared to the conventional Morinda officinalis, thereby allowing consumers to enjoy it without burden. Attached Figure Description

[0084] Figure 1 A graph representing the immobility time of experimental animals in the control group, single group, and experimental animals based on the intake of the composite composition in a forced swimming experiment according to the present invention.

[0085] Figure 2 A graph representing the immobility time of experimental animals in the control group, single group, and experimental animals based on the intake of the composite composition in the tail suspension experiment.

[0086] Figure 3 The graphs represent the experimental results of changes in blood IgA levels in mice under fixed pressure-induced conditions, including control, single-group, and composite composition-based uptake.

[0087] Figure 4 The graphs represent the experimental results of weight changes in the control group, single group, and compound composition intake of the present invention in mice under fixed pressure-induced stress.

[0088] Figure 5 The graphs represent the experimental results of changes in serotonin production in the control group, single group, and compound composition based on the intake of the present invention in mice under constant pressure.

[0089] Figure 6 The graphs represent the experimental results of changes in blood cortisol production in mice under constant pressure, including the control group, the single group, and the group based on the intake of the composite composition. Detailed Implementation

[0090] The present invention will now be described in more detail through the following embodiments. However, these embodiments are for illustrative purposes only, and the scope of the invention is not limited to these embodiments.

[0091] Example 1: Preparation of a composition for pressure relief

[0092] The composition according to the invention is prepared by the following process.

[0093] 1.1: Preparation of fermented aged noni

[0094] 690 kg of noni fruit was inoculated with a compound lactic acid bacteria (7 strains of compound lactic acid bacteria AON1805, (strain) Letumisen) and fermented at 37°C for more than 45 days. From the start of fermentation, 10 L of fermentation broth was collected every 7 days, transferred to the top of the noni fruit and spread, to carry out fermentation and aging. The composition of the compound lactic acid bacteria (7 strains of compound lactic acid bacteria AON1805, (strain) Letumisen) used at this time is shown in Table 1 below.

[0095] After the aging is completed, the fermentation-aged Noni juice is filtered to remove solids to obtain a fermentation-aged Noni extract.

[0096] [Table 1]

[0097]

[0098] 1.2: Preparation of a Kumquat extract

[0099] The Kumquats used in this experimental example were purchased from the market. Frozen or dried Kumquats were used by purchasing them from the market.

[0100] The frozen Kumquats were prepared into an extract by pulverizing and juicing. After 100 g of dried Kumquats were added with 10 times the amount of water by weight and extracted twice within 8 hours at 50°C, the extract was filtered using filter paper. The water in the filtrate was removed using a rotary vacuum evaporator (EYELA A-1000S, Tokyo Rikakikai Co., Tokyo, Japan) on a water bath at 37-40°C to prepare an extract, which was used as a feed.

[0101] The extraction yields of different samples of Kumquats using different extraction solvents are shown in the following [Table 2].

[0102] [Table 2]

[0103]

[0104] 1.3: Preparation of a complex using a fermentation-aged Noni extract and a Kumquat extract

[0105] After the fermentation-aged Noni extract and the Kumquat extract prepared in 1.1 to 1.2 above were mixed in different proportions, the complex was prepared by aging for 15 days.

[0106] [Preparation of a complex]

[0107] Complex 1: Fermentation-aged Noni extract (wt%) 90: Dried Kumquat extract (wt%) 10

[0108] Complex 2: Fermentation-aged Noni extract (wt%) 80: Dried Kumquat extract (wt%) 20

[0109] Complex 3: Fermentation-aged Noni extract (wt%) 70: Dried Kumquat extract (wt%) 30

[0110] Compound 4: Fermented aged Morinda citrifolia extract (wt%) 70: Frozen Citrus aurantium extract (wt%) 30

[0111] Compound 5: Fermented aged Morinda citrifolia extract (wt%) 70: Frozen Citrus aurantium extract (wt%) 30

[0112] Compound 6: Fermented aged Morinda citrifolia extract (wt%) 70: Frozen Citrus aurantium extract (wt%) 30

[0113] Experimental Example 1: Confirmation of the effect of the composition of the present application on stress release

[0114] The effect of the single extracts prepared by the above Examples 1.1 to 1.2 and the compound prepared by Example 1.3 on stress release ability was confirmed.

[0115] (1) Test object of mice

[0116] ICR male mice (Ossian Korea, Korea) of 4 weeks of age, after a week of adaptation period, the lighting and extinguishing of the lighting equipment were repeated in 12 hour units, after adapting in a space maintaining the room temperature of 18 to 23°C and humidity of 60% for a week, the experimental animals were classified into each of 8 as follows. As a feed, solid feed was supplied, except for the process of inducing stress, it was not limited to the feed and water supply.

[0117] Experimental group 1 (normal control group, hereinafter Normal), experimental group 2 (negative control group, hereinafter Control) were orally administered with physiological saline. On the other hand, it was set to experimental group 3 (fermented aged Morinda citrifolia 100 mg / kg administration group, hereinafter FN), experimental group 4 (dried Citrus aurantium extract 100 mg / kg administration group, hereinafter DCE), experimental group 5 (frozen Citrus aurantium extract 100 mg / kg administration group, hereinafter FCE), experimental group 6 (compound 1 100 mg / kg administration group, hereinafter CI), experimental group 7 (compound 2 100 mg / kg administration group, hereinafter C2), experimental group 8 (compound 3 100 mg / kg administration group, hereinafter C3), experimental group 9 (compound 4 100 mg / kg administration group, hereinafter C4), experimental group 10 (compound 5 100 mg / kg administration group, hereinafter C5), experimental group 11 (compound 6 100 mg / kg administration group, hereinafter C6), and the test substance was administered in total for 4 weeks at 1 time / 1 day.

[0118] (2) Induction of stress

[0119] The negative control group for the above subjects used physiological saline. Experimental groups 1, 2, 3, 4, 5, and 6 underwent daily stress induction for four weeks starting seven days after administration of a single extract or compound. The stress induction method was modified from that of Willner et al. (Wilner et al., Reduction of sucrose preference by chronic predictable mild stress, 1987), specifically inducing various unpredictable psychological stress conditions such as fasting, post-fasting dietary restrictions (providing small amounts of feed), water deprivation, providing empty bottles after water deprivation, tilted enclosures, housing multiple experimental animals in one enclosure, flickering lights, cold rooms, and continuous lighting, thereby inducing stress.

[0120] (3) Forced Swimming Test

[0121] Forced swimming experiments were conducted on laboratory animals. A cylindrical transparent tank (14 cm in diameter, 20 cm in height) was filled with water at 23–25°C until the water depth reached 15 cm. Each animal was then placed in the tank. All animals were forced to swim in the cylindrical tank for 6 minutes. The first 2 minutes were considered the adaptation time. In the last 4 minutes, the total time the animals remained stationary in the cylindrical tank (hereinafter referred to as the "stationary time") was measured and recorded.

[0122] Specifically, immobility time refers to a state of complete stillness, characterized by no movement other than minor movements such as raising one's head to the surface of the water. Immobility is one of the indicators of despair in depression and is frequently observed in stress-related experimental animals.

[0123] like Figure 1 As shown, the immobilization time in the negative control group (Control) was longer than that in the normal control group (Normal). Conversely, the immobilization time in experimental group 3 was slightly longer than that in the normal control group, but it was still shorter than that in the negative control group at the 80% level. Conversely, experimental groups 4 and 5 showed significant differences compared to the negative control group, and the immobilization time in the experimental groups (6, 7, 8, 9, 10, and 11) treated with the fermented aged noni and calamansi complex was significantly reduced by half compared to the negative control group. Therefore, it can be concluded that the complex is more effective in reducing immobilization time than single extracts of fermented aged noni or calamansi.

[0124] (4) Tail Suspension Test

[0125] Two times of tail suspension test were performed on experimental animal subjects. The experimental animal was hung upside down by holding the 1 cm end of the tail from the bottom to a height of 15 cm for 6 minutes, and the movement of the experimental animal was observed. The initial 2 minutes were regarded as an adaptation time, and the time during which the experimental animal was motionless was measured and recorded within the last 4 minutes.

[0126] As shown in FIG. 6, it was investigated that the time of being motionless in the state of being hung upside down was longer in the negative control group (Control) than in the normal control group (Normal). This means that the time of being motionless was increased due to mental stress, and the feeling of frustration was increased. Figure 2

[0127] The time of being motionless in the experimental group 3 (FN) was shorter than that in the negative control group (Control) at a level of 82%, and the body was more mobile, but the time of being motionless in the experimental group 4 was significantly different from that in the negative control group at a level of 92%.

[0128] Also, it was investigated that the experimental groups of the complexes (1, 2, 3, 4, 5, and 6) prepared by mixing the fermentation-aged Morinda citrifolia extract (FN) and the Citrus kawarazamensis extract (DCE & FCE) extract at a prescribed ratio were significantly lower than the negative control group. It was thus known that the complex of the fermentation-aged Morinda citrifolia extract and the Citrus kawarazamensis extract mixed at a prescribed ratio was more effective in relieving the feeling of powerlessness and frustration due to stress.

[0129] Experimental Example 2: Experiment for inducing changes in the IgA level in the blood of a mouse based on the ingestion of a composition under fixed stress

[0130] The 8-week-old C57BL / 6 mice were orally administered 100 mg / kg of the complexes (1, 2, 3, 4, 5, and 6) prepared by mixing the fermentation-aged Morinda citrifolia extract (FN), the Citrus kawarazamensis extract (CE), the fermentation-aged Morinda citrifolia extract (FN), and the Citrus kawarazamensis extract (DCE & FCE) extract at a prescribed ratio every day for 21 days in a narrow frame in which the mice were not allowed to move for 2 hours per day.

[0131] As shown in FIG. 7, the results of measuring the IgA level in the serum showed that the IgA level was statistically significantly increased in the fermentation-aged Morinda citrifolia extract administration group (FN), the complex 1 administration group, and the complex 4 administration group compared to the control group (control). Figure 3 In particular, the highest increase in the IgA level was shown in the complex 4 administration group.

[0132] Experimental Example 3: Experiment for inducing changes in the body weight of a mouse based on the ingestion of a complex under fixed stress

[0133]

[0134] ​​The complexes (1, 2, 3, 4, 5, and 6) prepared by mixing the fermented and aged Morinda citrifolia extract (FN), the Citrus kamanensis extract (CE), the fermented and aged Morinda citrifolia extract (FN) and the Citrus kamanensis extract (DCE & FCE) at a predetermined ratio were orally administered to 8-week-old C57BL / 6 at 100 mg / kg per day for 21 days at 2 hours per day to induce a fixed stress.

[0135] As shown in Table 1, the results of the body weight change measurement showed that the body weight of the fixed control group was reduced compared to that of the normal group (Normal), and the body weight reduction of the fermented and aged Morinda citrifolia extract (FN), the complex 1, and the complex 4 administration groups was lower than that of the fixed control group (control). Figure 4

[0136] In particular, the lowest body weight reduction was shown in the complex 1 and the complex 4.

[0137] Experimental Example 4: Measurement experiment of the change in the production amount of Cortisol and Serotonin in the blood of a stress-induced mouse based on the intake of a complex

[0138] The complexes (1, 2, 3, 4, 5, and 6) prepared by mixing the fermented and aged Morinda citrifolia extract (FN), the Citrus kamanensis extract (CE), the fermented and aged Morinda citrifolia extract (FN) and the Citrus kamanensis extract (DCE & FCE) at a predetermined ratio were orally administered to 8-week-old C57BL / 6 at 100 mg / kg per day for 21 days at 2 hours per day to induce a fixed stress.

[0139] The results of the measurement of the level of Serotonin, a hormone that feels happy emotions in the serum, showed that the level of Serotonin was statistically significantly increased in the fermented and aged Morinda citrifolia extract (FN) 100 mg / kg (p<0.05) and the fermented and aged Morinda citrifolia extract (FN), the complex 1, and the complex 4 administration groups compared to the fixed control group (Control) Figure 5 .

[0140] The results of the measurement of the level of Cortisol, a stress hormone in the serum, showed that the level of Cortisol was statistically significantly reduced in the fermented and aged Morinda citrifolia extract (FN) and the complex 1 and 4 administration groups compared to the fixed control group (control) Figure 6 .

[0141] In particular, the highest increase in the level of Serotonin and the highest reduction in the level of Cortisol were shown in the complex 1 and the complex 4.

[0142] ​It will be apparent from the foregoing description that a person of ordinary skill in the art of the present application can embody the present application in specific forms other than those set forth herein without departing from the technical idea or essential characteristics of the present application. To that end, nothing described herein should be interpreted as limiting the scope of the present application. The embodiments described hereinabove are exemplary in all aspects, not limiting. The scope of the present application is defined by the appended claims as meaning and scope thereof and all modifications or variations derived from the equivalent concepts thereof included in the scope of the present application.

Claims

1. A food composition comprising fermented aged noni or an extract thereof as active ingredients and dried calamansi extract, The weight ratio of the fermented aged noni or its extract to the dried calamansi extract is 90:

10.

2. The food composition according to claim 1, wherein, The fermented and aged noni or its extract is obtained by fermenting and aging noni using lactic acid bacteria.

3. The food composition according to claim 1 or 2, wherein, The food composition is a health food.

4. The food composition according to claim 1, wherein, The food composition is selected from any dosage form of powder, granules, pills, tablets, capsules, candy, syrup and beverage.

5. A pharmaceutical composition for the prevention or treatment of stress-induced diseases, comprising fermented aged noni or an extract thereof as active ingredients and dried calamansi extract. The weight ratio of the fermented aged noni or its extract to the dried calamansi extract is 90:

10. The stress-induced illnesses mentioned are depression, anxiety, chronic fatigue syndrome, sleep disorders, panic disorder, memory loss, asthenia, or insomnia.

6. A quasi-pharmaceutical composition for stress relief or for the prevention or treatment of stress-induced disorders, comprising fermented aged noni or an extract thereof as active ingredients and dried calamansi extract. The weight ratio of the fermented aged noni or its extract to the dried calamansi extract is 90:

10. The stress-induced illnesses mentioned are depression, anxiety, chronic fatigue syndrome, sleep disorders, panic disorder, memory loss, asthenia, or insomnia.

7. A feed composition for stress relief, comprising fermented aged noni or an extract thereof as active ingredients and dried calamansi extract. The weight ratio of the fermented aged noni or its extract to the dried calamansi extract is 90:

10.

8. A method for preparing a composition for pressure release comprising fermented aged noni or an extract thereof and dried calamansi extract, the method comprising the following steps: Step (a) Obtain fermented and aged noni using lactic acid bacteria; as well as Step (b): Add dried calamansi oranges to the fermented and aged noni, then age it. The weight ratio of the fermented aged noni or its extract to the dried calamansi extract is 90:

10.

9. The method according to claim 8, further comprising, after step (a), juicing the fermented aged noni obtained in step (a) to obtain fermented aged noni extract.

10. A composition obtained by the method according to claim 8.

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