Compound health-care capsule containing roxburgh rose fine powder and preparation method of compound health-care capsule

Abstract

The invention relates to the field of composite health-care capsules, and particularly discloses a composite health-care capsule containing roxburgh rose fine powder and a preparation method of the composite health-care capsule. The capsule is prepared from the following raw materials in percentage by weight: 80% of roxburgh rose fine powder, 10% of garlic extract and 10% of mushroom extract. The roxburgh rose fine powder, the garlic extract and the mushroom extract are compounded, and an optimized soft capsule preparation process is adopted, so that stable coexistence and efficient delivery of all functional components in an oily medium are successfully realized; rosa roxburghii tratt fine powder is used as a core substrate, and the rosa roxburghii tratt fine powder is rich in natural vitamin C and SOD active ingredients, and cooperates with allicin in the garlic extract and lentinan in the lentinus edodes extract to jointly construct a multi-layer and multi-target composite health-care system; the system not only significantly enhances the overall biological activity of the product, but also breaks through the limitation of a single component in efficacy through the complementation and gain effects of all the components.

Description

TECHNICAL FIELD

[0001] The present application belongs to the field of composite health capsules, in particular to a composite health capsule containing rose apple fine powder and a preparation method thereof. BACKGROUND

[0002] With the improvement of health awareness, the development of health food using natural plant active ingredients has become an important trend. Rose apple, as a fruit of medicinal and edible origin, is rich in vitamin C, superoxide dismutase (SOD) and other antioxidant ingredients, and shows potential value in enhancing immunity and anti-fatigue. Garlicin in garlic extract and lentinan in shiitake extract are also widely studied and applied due to their unique physiological activities, such as regulating blood lipids, antibacterial and anti-inflammatory, and immune enhancement. Scientifically compounding these natural active ingredients with complementary functions to achieve synergistic effect is an ideal direction for the development of health food.

[0003] In terms of technical implementation, soft capsule dosage form is often used for the development of such composite health products because it can effectively mask unpleasant odors, improve ingredient stability, and facilitate the packaging of oil-soluble or suspended contents.

[0004] The existing composite health capsules cannot fundamentally solve the problems of physical stability and bioavailability of multiple active ingredients in the composite system, and it is even more difficult to ensure stable and predictable synergistic effect between components, resulting in that the comprehensive health efficacy of the final product cannot reach the theoretical expectation, and the product quality uniformity is also difficult to guarantee. SUMMARY

[0005] To solve the above technical problems, the present application provides a composite health capsule containing rose apple fine powder to solve the problems of insufficient physical stability and bioavailability of multiple active ingredients, difficulty in fully exerting the synergistic effect between components, and poor comprehensive efficacy and quality uniformity of the product in the prior art.

[0006] A composite health capsule containing rose apple fine powder is composed of the following raw materials in weight percentage:

[0007] Rose apple fine powder 80%, garlic extract 10%, shiitake extract 10%.

[0008] Preferably, the preparation method of the rose apple fine powder is prepared by Lactobacillus buchneri fermentation, and the content of vitamin C is not less than 13000 mg / 100 g, and the content of SOD is not less than 100000 u / g.

[0009] Preferably, the content of garlicin in the garlic extract is not less than 10%.

[0010] Preferably, the content of lentinan in the shiitake extract is not less than 50%.

[0011] Preferably, the content of the capsule is prepared by mixing the fine powder mixture with soybean oil at a weight ratio of 1:1, and uniformly grinding to form a uniform suspension.

[0012] A method for preparing the compound health-care capsule containing the roxburgh rose fine powder as described above, comprising the following steps:

[0013] S1, batching: mixing the roxburgh rose fine powder, garlic extract and shiitake extract in proportion, stirring uniformly and crushing to 100 mesh or more to obtain a fine powder mixture;

[0014] S2, mixing: mixing the fine powder mixture with soybean oil at a weight ratio of 1:1, and uniformly grinding to make the fine powder uniformly suspended in the soybean oil;

[0015] S3, gelation: placing gelatin, glycerol and water in a weight ratio of 1:0.35:0.75 in a double boiler, heating and stirring until completely dissolved, and keeping warm for 1-2 hours. After removing the foam, filter to obtain a gel slurry;

[0016] S4, pressing the capsule: preparing the gel slurry into a gel plate or tape, and filling the liquid medicine between the two tapes by pressing or automatic rotating capsule machine, and then pressing, cutting, cooling, ethanol washing, drying to obtain soft capsules.

[0017] Preferably, the preparation environment conditions of the soft capsule in step S4 are:

[0018] The temperature of the soft capsule process room and the drying room is 22-24℃, and the relative humidity is 20%;

[0019] The temperature of the soft capsule detection room is 22-24℃, and the relative humidity is 35%.

[0020] Preferably, the weight of the prepared capsule is 0.75g / particle, the weight of the content is 0.55g / particle, and the weight of the fine powder is 0.3g / particle.

[0021] Compared with the prior art, the present application has the following beneficial effects:

[0022] By compounding the roxburgh rose fine powder, garlic extract and shiitake extract, and using the optimized soft capsule preparation process, the stable coexistence and efficient delivery of each functional component in the oil medium are successfully realized;

[0023] As the core matrix, the roxburgh rose fine powder is rich in natural vitamin C and SOD active ingredients, which are synergistic with allicin in garlic extract and lentinan in shiitake extract, together forming a multi-level and multi-target compound health-care system. This system not only significantly enhances the overall biological activity of the product, but also breaks through the limitations of single component in efficacy through the complementary and gain effect of each component;

[0024] In addition, the paste formula and the precisely controlled pressure capsule environment adopted by the present application effectively ensure that the final product has good morphological stability, a suitable disintegration time limit and quality uniformity during long-term storage. Therefore, the present application exhibits significant beneficial effects in improving the comprehensive physiological regulation function of health-care capsules, enhancing product stability and meeting the quality control requirements of industrial production. BRIEF DESCRIPTION OF DRAWINGS

[0025] Figure 1 A schematic diagram of the method of the present application. DETAILED DESCRIPTION

[0026] The technical solutions in the embodiments of the present application will be described clearly and completely below with reference to the accompanying drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative labor fall within the scope of protection of the present application.

[0027] As shown in Figure 1

[0028] The Malus hallaisanensis fine powder adopted in the embodiments of the present application is prepared according to the preparation method of the patent “Malus hallaisanensis and application thereof in preparation of Malus hallaisanensis fruit powder” (Patent No. ZL202410372284.0);

[0029] The garlic extract is purchased from Shanxi Ruishi Biological Technology Co., Ltd.

[0030] The shiitake mushroom extract is purchased from Fujian Sanming Herbal Treasure Biological Engineering Co., Ltd.

[0031] Embodiment 1: The present embodiment provides a compound health-care capsule containing Malus hallaisanensis fine powder and a preparation method thereof.

[0032] 1. Raw material ratio:

[0033] Malus hallaisanensis fine powder: 80%, garlic extract: 10%, shiitake mushroom extract: 10%;

[0034] The content of vitamin C in the Malus hallaisanensis fine powder is 13000 mg / 100 g, and the content of SOD is 100000 u / g.

[0035] The content of allicin in the garlic extract is 10%.

[0036] The content of lentinan in the shiitake mushroom extract is 50%.

[0037] 2. Preparation method:

[0038] ​S1, batching: the raw materials are weighed according to the above-mentioned proportions, mixed, stirred uniformly, and pulverized to 100 meshes to obtain a fine powder mixture.

[0039] S2, mixing: the obtained fine powder mixture is mixed with soybean oil at a weight ratio of 1:1, and is uniformly ground by a colloid mill to make the fine powder uniformly suspended in the soybean oil to obtain a medicinal liquid.

[0040] S3, gelation: the gelatin, glycerol and water are placed in a jacketed kettle at a weight ratio of 1:0.35:0.75, heated by steam and continuously stirred until completely dissolved. Then, under the condition of heat preservation, it is placed for 1 hour, and after the foam floats, it is filtered under heat preservation to obtain a gelatin paste.

[0041] S4, capsule compression: the production is carried out by compression method. The gelatin paste is made into adhesive tape, the above-mentioned medicinal liquid is placed between two adhesive tapes, the thickness of the adhesive skin is adjusted to the lower limit of the process requirement, and the steel mold is used for compression. The prepared gelatin capsule is first cooled and fixed, then washed with ethanol to remove oil, and finally dried.

[0042] Environmental control: the temperature of the soft capsule process room and the drying room is controlled at 22°C, and the relative humidity is controlled at 20%; the temperature of the soft capsule detection room is controlled at 22°C, and the relative humidity is controlled at 35%.

[0043] Quality index: the weight of the finally prepared capsule is 0.75g / pill, the weight of the content is 0.55g / pill, and the weight of the fine powder is 0.3g / pill.

[0044] Example 2: The present embodiment provides a compound health care capsule containing rose apple fine powder and a preparation method thereof.

[0045] 1. Raw material ratio:

[0046] Rose apple fine powder: 80%, garlic extract: 10%, and shiitake mushroom extract: 10%;

[0047] The content of vitamin C in the rose apple fine powder is 15000mg / 100g, and the content of SOD is 120000u / g.

[0048] The content of allicin in the garlic extract is 12%.

[0049] The content of lentinan in the shiitake mushroom extract is 55%.

[0050] 2. Preparation method:

[0051] S1, batching: the raw materials are weighed according to the above-mentioned proportions, mixed, stirred uniformly, and pulverized to 120 meshes to obtain a fine powder mixture.

[0052] S2, mixing: the obtained fine powder mixture is mixed with soybean oil at a weight ratio of 1:1, and is uniformly ground by a colloid mill to make the fine powder uniformly suspended in the soybean oil to obtain a medicinal liquid.

[0053] S3, gelation: gelatin, glycerol and water were placed in a sandwich pot in a weight ratio of 1:0.35:0.75, heated with steam and continuously stirred until completely dissolved. Then, under the condition of heat preservation, it was placed for 1.5 hours, and after the foam floated, it was filtered under heat preservation to obtain a gelatin slurry.

[0054] S4, capsule compression: continuous production was carried out by using an automatic rotary capsule machine. The two machines automatically made the adhesive tape move in opposite directions, and when they reached the rotary die, a part of the adhesive tape had been combined under pressure. At this time, the liquid medicine was introduced into the adhesive tape from the filling pump through the catheter, and the rotary die was rotated to make the liquid medicine enter the groove. Then, the adhesive tape was completely combined by rolling, and the excess adhesive tape was cut. The prepared capsule was first cooled and fixed, then washed with ethanol to remove oil, and finally dried.

[0055] Environmental control: the temperature of the soft capsule process room and the drying room was controlled at 23°C, and the relative humidity was controlled at 20%; the temperature of the soft capsule detection room was controlled at 23°C, and the relative humidity was controlled at 35%.

[0056] Quality index: the weight of the finally prepared capsule is 0.75g / pill, the weight of the content is 0.55g / pill, and the weight of the fine powder is 0.3g / pill.

[0057] Example 3: The present embodiment provides a compound health care capsule containing rosehip fine powder and a preparation method thereof.

[0058] 1. Raw material ratio:

[0059] Rosehip fine powder: 80%, garlic extract: 10%, and shiitake mushroom extract: 10%;

[0060] The content of vitamin C in the rosehip fine powder is 17000mg / 100g, and the content of SOD is 140000u / g.

[0061] The content of allicin in the garlic extract is 14%.

[0062] The content of lentinan in the shiitake mushroom extract is 60%.

[0063] 2. Preparation method:

[0064] S1, batching: the raw materials were weighed according to the above proportions, mixed, stirred uniformly, and pulverized to more than 150 mesh to obtain a fine powder mixture.

[0065] S2, mixing: the obtained fine powder mixture was mixed with soybean oil in a weight ratio of 1:1, and was ground uniformly by a colloid mill to make the fine powder uniformly suspended in the soybean oil to obtain a liquid medicine.

[0066] S3, gelation: gelatin, glycerin and water were placed in a sandwich pot according to the weight ratio of 1:0.35:0.75, heated by steam and continuously stirred until completely dissolved. Then, under the condition of heat preservation, it was placed for 2 hours. After the foam floated, it was filtered under heat preservation to obtain the gelatin slurry.

[0067] S4, capsule compression: continuous production was carried out by using automatic rotary capsule machine. The two machines automatically made the adhesive tape move in opposite directions. When it reached the rotary die, a part of the adhesive tape had been combined under pressure. At this time, the liquid medicine was introduced into the adhesive tape from the filling pump through the catheter. The rotary die rotated to make the liquid medicine enter the groove. Then, the adhesive tape was completely combined by rolling pressure, and the excess adhesive tape was cut. The prepared capsule was first cooled and fixed, then washed with ethanol to remove oil, and finally dried.

[0068] Environmental control: the temperature of the soft capsule process room and the drying room was controlled at 24°C, and the relative humidity was controlled at 20%; the temperature of the soft capsule detection room was controlled at 24°C, and the relative humidity was controlled at 35%.

[0069] Quality index: the weight of the finally prepared capsule was 0.75g / pill, the weight of the content was 0.55g / pill, and the weight of the fine powder was 0.3g / pill.

[0070] Experimental example: evaluation of the in vitro antioxidant activity of the compound health care capsule.

[0071] 1. Experimental purpose:

[0072] The purpose of this experiment is to evaluate the antioxidant capacity of the compound health care capsule with Example 2 as a reference through an in vitro antioxidant model, and to verify the scientificity and synergistic effect of the combination of rose hip fine powder, garlic extract and shiitake mushroom extract by setting different comparative examples.

[0073] 2. Experimental materials and grouping:

[0074] Reference group: the content of the compound health care capsule prepared according to the method of Example 2 (rose hip fine powder 80% + garlic extract 10% + shiitake mushroom extract 10%).

[0075] Comparative example 1: containing only rose hip fine powder (100% rose hip fine powder).

[0076] Comparative example 2: binary combination of rose hip fine powder and garlic extract (rose hip fine powder 80% + garlic extract 20%).

[0077] Comparative example 3: binary combination of rose hip fine powder and shiitake mushroom extract (rose hip fine powder 80% + shiitake mushroom extract 20%).

[0078] Comparative example 4: binary combination without rose hip fine powder (garlic extract 50% + shiitake mushroom extract 50%).

[0079] Positive control group: ascorbic acid (Vc) aqueous solution.

[0080] Blank control group: equal volume of dissolved soybean oil or corresponding solvent.

[0081] All test samples were accurately weighed, dissolved or diluted with appropriate solvent (such as absolute ethanol or PBS buffer), and prepared into the same fine powder concentration (such as 10 mg / mL) of the test solution.

[0082] 3. Experimental scheme:

[0083] The DPPH free radical scavenging experiment and the FRAP iron ion reducing capacity experiment were used to comprehensively evaluate the antioxidant activity of the samples. Each experiment was independently repeated three times.

[0084] 3.1 DPPH free radical scavenging experiment:

[0085] Principle: DPPH is a stable free radical, and its ethanol solution is purple with a maximum absorption peak at 517 nm. Antioxidants can provide hydrogen atoms to fade the color, and the fading degree is positively correlated with the antioxidant capacity.

[0086] Steps: Take 2 mL of 0.1 mM DPPH ethanol solution, mix with 0.5 mL of different groups of test solution, vortex and shake, and react at room temperature for 30 minutes. Then measure the absorbance value (As) at 517 nm wavelength. At the same time, measure the absorbance of 0.5 mL of test solution mixed with 2 mL of absolute ethanol (Aj), and the absorbance of 0.5 mL of solvent mixed with 2 mL of DPPH solution (Ac).

[0087] Calculation: Scavenging rate (%) = [1-(As-Aj) / Ac] x 100%.

[0088] 3.2 FRAP iron ion reducing capacity experiment:

[0089] Principle: Under acidic conditions, antioxidants can reduce Fe 3+ - TPTZ complex to blue Fe 2+ Form, which has a maximum absorption peak at 593 nm, and its absorbance value is positively correlated with the total reducing capacity.

[0090] Steps: Prepare the FRAP working solution according to the kit instructions. Take an appropriate amount of FRAP working solution and mix with the test solution, incubate at 37°C for 10 minutes, and measure the absorbance value at 593 nm wavelength. Make a standard curve with ferrous sulfate (FeSO4) solution, and the result is expressed as the equivalent amount of FeSO4 per gram of sample (mg FeSO4 / g).

[0091] 4. Experimental table:

[0092] Table 1: Comparison of DPPH radical scavenging rate and FRAP value of each experimental group

[0093] Group Ingredient Description DPPH Scavenging Rate (%) FRAP value (mg FeS04 / g) Reference Group Rosa roxburghii Tratt 80% + Allium sativum L. 10% + Lentinula edodes 10% 88.5±2.1 425.6±15.3 Comparative Example 1 100% Rosa roxburghii Tratt Fine Powder 75.2±1.8 380.4±12.7 Comparative Example 2 Rosa roxburghii Tratt 80% + Allium sativum L. 20% 82.3±2.0 405.8±14.1 Comparative Example 3 Rosa roxburghii Tratt 80% + Lentinula edodes 20% 84.1±1.9 418.9±13.5 Comparative Example 4 Allium sativum L. 50% + Lentinula edodes 50% 25.7±1.2 85.2±8.5 Positive Control Ascorbic Acid (Vc) 95.8±0.5 - Blank Control Solvent 2.1±0.5 5.5±1.2

[0094] 5. Analysis of experimental results:

[0095] As shown in the data, Comparative Example 1 (pure Rosa roxburghii powder) has shown strong antioxidant activity (DPPH scavenging rate 75.2%, FRAP value 380.4 mg / g), which is mainly due to the high content of vitamin C and SOD in the Rosa roxburghii powder, which has high antioxidant capacity.

[0096] The antioxidant indexes of Comparative Example 2 (Rosa roxburghii + garlic) and Comparative Example 3 (Rosa roxburghii + Lentinus edodes) are significantly higher than those of Comparative Example 1. This shows that whether adding garlic extract or Lentinus edodes extract can further improve the overall antioxidant level of the system on the basis of Rosa roxburghii powder.

[0097] The DPPH scavenging rate and FRAP value of the reference group (ternary compound formula) are the highest, which is significantly better than any one of the binary combinations (Comparative Example 2 and Comparative Example 3). It proves that there is a significant synergistic effect between Rosa roxburghii powder, garlic extract and Lentinus edodes extract.

[0098] The antioxidant activity of Comparative Example 4 (garlic + Lentinus edodes, without Rosa roxburghii) is very low, which shows that in the absence of the core component Rosa roxburghii powder, the direct contribution of garlic and Lentinus edodes extract to the total antioxidant capacity in the experimental system is small.

[0099] 6. Experimental conclusion:

[0100] The in vitro antioxidant evaluation of the experiment shows that the compound health care capsule (Example 2) composed of 80% Rosa roxburghii powder, 10% garlic extract and 10% Lentinus edodes extract provided by the present application has a significantly better antioxidant capacity than single component or any binary combination. The ternary formula shows a clear synergistic effect.

[0101] In the description of the specification, the description of the terms "one embodiment", "some embodiments", "example", "specific example" or "some examples" means that the specific features, structures, materials or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present application. In the specification, the illustrative description of the above terms is not necessarily for the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner. In addition, the skilled in the art can combine and combine the different embodiments or examples described in the specification and the features of the different embodiments or examples without contradiction.

[0102] In the drawings of the embodiments of the present application, only the structures related to the embodiments of the present application are involved, and other structures can be referred to the general design. In the case of no conflict, the same embodiments and different embodiments of the present application can be combined with each other.

[0103] Although the present application has been described in detail with reference to the foregoing embodiments, those skilled in the art can modify the technical solutions recorded in the foregoing embodiments, or make equivalent replacements to some technical features, and any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A composite health capsule containing a powdered Physalis fructus, characterized in that, It is composed of the following raw materials in weight percentage: Rosa roxburghii Tratt. fine powder 80%, garlic extract 10%, and Lentinula edodes extract 10%.

2. The composite health capsule according to claim 1, wherein The preparation method of the Rosa roxburghii Tratt. fine powder is fermentation by Brochothrix camini, and the content of vitamin C is not less than 13000 mg / 100 g, and the content of SOD is not less than 100000 u / g.

3. The composite health capsule according to claim 1, wherein The content of allicin in the garlic extract is not less than 10%.

4. The composite health capsule according to claim 1, wherein The content of lentinan in the Lentinula edodes extract is not less than 50%.

5. The composite health capsule according to claim 1, wherein The content of the capsule is a uniform suspension prepared by mixing the fine powder mixture and soybean oil at a weight ratio of 1:1 and grinding by a colloid mill.

6. A method of preparing a composite health capsule containing a powdered roll gourd extract as claimed in any one of claims 1 to 5, wherein, It comprises the following steps: S1, batching: mixing the Rosa roxburghii Tratt. fine powder, garlic extract, and Lentinula edodes extract in proportion, stirring uniformly and crushing to more than 100 mesh to obtain a fine powder mixture; S2, mixing: mixing the fine powder mixture and soybean oil at a weight ratio of 1:1, grinding uniformly by a colloid mill, and uniformly suspending the fine powder in the soybean oil; S3, gelation: placing gelatin, glycerol, and water in a weight ratio of 1:0.35:0.75 in a jacketed kettle, heating and stirring until completely dissolved, keeping warm for 1-2 hours, filtering after removing foam to obtain a gelatin paste; S4, pressing capsules: preparing the gelatin paste into a gelatin plate or tape, filling the liquid medicine between two gelatin tapes by pressing or automatic rotating capsule machines, and pressing, cutting, cooling, ethanol washing, and drying to obtain soft capsules.

7. The method of claim 6, wherein, The preparation environment conditions of the soft capsules in step S4 are: The temperature of the soft capsule process room and the drying room is 22-24℃, and the relative humidity is 20%; The temperature of the soft capsule detection room is 22-24℃, and the relative humidity is 35%.

8. The method of claim 6, wherein, The weight of the prepared capsules is 0.75 g / piece, the weight of the content is 0.55 g / piece, and the weight of the fine powder is 0.3 g / piece.

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