Buccal cigarette containing whey protein and preparation method thereof

Abstract

The invention relates to the technical field of combination of tobacco products and functional nutrition supplement, in particular to a whey protein-containing buccal cigarette and a preparation method thereof, the whey protein-containing buccal cigarette comprises a dephenolized low-irritation tobacco extract, hydrolyzed whey protein, a muscle-building auxiliary agent, a slow-release filler, a functional sweetening agent, natural perfume, a biocompatible adhesive and a moisturizing and solubilizing auxiliary agent, the weight ratio of the hydrolyzed whey protein to the muscle-building auxiliary agent is controlled, and the method comprises the steps of tobacco extract extraction, muscle-building nutrition premix preparation, total material mixing, slow-release coating, tabletting forming and light-shielding packaging. The product provided by the invention realizes the dual functions of tobacco consumption and instant muscle-building nutrition supplement, can reduce the influence of nicotine on proteolysis, prolong the release time of protein and improve palatability, also uses biocompatible raw materials to improve safety, and solves the problem that the existing buccal tobacco lacks muscle-building function.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of tobacco products and functional nutrition supplement, and particularly relates to a mouth tobacco containing whey protein and a preparation method thereof. BACKGROUND

[0002] With the improvement of health awareness and fitness needs, products combining tobacco consumption experience and functional nutrition supplement have become a new direction in the market. As a smokeless tobacco product, mouth tobacco avoids smoke hazards, but existing products only focus on taste and basic safety, and do not associate the core needs of sports groups, i.e., muscle gain.

[0003] Sports groups need high-quality protein for timely supplementation, and whey protein is an ideal source of muscle gain due to its high content of branched-chain amino acids (BCAA) (20%-25% of total amino acids) and fast digestion and absorption rate (biological value of 104, higher than 77 of casein protein). However, existing mouth tobacco has two major defects: first, the amount of whey protein added is low (mostly less than 10%), and muscle gain auxiliary ingredients are not matched, which cannot meet the nutritional needs of muscle synthesis; second, nicotine in tobacco extract may accelerate protein decomposition, and if the ingredient ratio is not optimized, it may affect muscle gain. Therefore, it is of important market value and practical significance to develop a mouth tobacco that can balance tobacco flavor, nicotine intake, and high-efficiency muscle gain nutrition supplementation. SUMMARY

[0004] The purpose of the present application is to provide a mouth tobacco containing whey protein, which optimizes the types and amounts of whey protein and matches muscle gain auxiliary ingredients, meets the needs of tobacco consumption, provides sufficient high-quality protein and muscle gain essential nutrients for the human body, inhibits the decomposition of nicotine on protein, and realizes the dual functions of "tobacco consumption + immediate muscle gain nutrition supplementation".

[0005] The technical solution adopted by the present application to solve its technical problems is: a mouth tobacco containing whey protein, comprising, by weight percentage:

[0006] 15%-30% of low-stimulation tobacco extract;

[0007] 15%-25% of hydrolyzed whey protein;

[0008] 5%-7% of muscle gain auxiliary agent;

[0009] 15%-25% of sustained-release filler;

[0010] 8%-11% of functional sweetener;

[0011] 2%-5% of natural flavor;

[0012] 1%-3% of biocompatible binder;

[0013] Moistening and solubilizing aids 3-7 wt.%;

[0014] The balance is deionized water.

[0015] The sum of the weight percentages of the components is 100%.

[0016] Further, the low-irritation tobacco extract has a nicotine content of 8-15 wt%, is prepared by supercritical CO2 extraction method, the extraction conditions are pressure 30 MPa, temperature 45 DEG C, time 2.5 hours, and is treated by polyvinylpyrrolidone, and polyphenols are removed by greater than or equal to 60%, and the promotion of protein decomposition is reduced.

[0017] Further, the hydrolyzed whey protein has a molecular weight of less than or equal to 3000 Da, a protein purity of greater than or equal to 90%, a BCAA content of greater than or equal to 23%, and a digestion and absorption rate that is 30% higher than that of ordinary whey protein.

[0018] Further, the muscle-building auxiliary agent with a weight percentage of 5-7% includes creatine 2-4%, glutamine 2-3%, and vitamin D3 0.001-0.003%, and synergistically promotes muscle synthesis, wherein the weight ratio of creatine to glutamine is 1:1-2:1.

[0019] Further, the weight ratio of the hydrolyzed whey protein to the muscle-building auxiliary agent is 3:1-5:1, so as to ensure that the protein supply matches the muscle synthesis demand.

[0020] Further, the slow-release filler is a mixture of hydroxypropyl starch and inulin in a weight ratio of 3:1, which prolongs the release time of the protein in the oral cavity, and the release time is greater than or equal to 40 minutes, and the absorption efficiency is improved.

[0021] Further, the functional sweetener with a weight percentage of 8-11% is a compound of 5-8% erythritol, 0.1-0.3% stevioside and 2-3% taurine, which relieves the fatigue caused by nicotine.

[0022] Further, the natural spice with a weight percentage of 2-5% is a mixture of 0.5-1% menthol and 1.5-4% citrus essential oil, which masks the fishy smell of the protein and improves palatability; the biocompatible binder is konjac glucomannan, which has good compatibility with whey protein, can delay the release of nicotine, and reduces the interference with protein metabolism; and the moistening and solubilizing aids are a mixture of 2-4% glycerol and 1-3% polyethylene glycol 400, which improves the solubility of the whey protein and prevents the product from drying and caking.

[0023] Another object of the present application is to provide a preparation method of the smokeless tobacco containing whey protein.

[0024] Step one: preparation of low irritation tobacco extract, supercritical CO2 extraction, dephenolization, concentration, spray drying to get powder;

[0025] Step two: preparation of muscle building nutrition premix, mix hydrolyzed whey protein and muscle building auxiliary agent under nitrogen protection, water content ≤3%;

[0026] Step three: mix the total material, first mix the sustained-release filler and moisture-solubility aid, then add the remaining ingredients, heat to 40°C and mix for 25 minutes to prepare a uniform material;

[0027] Step four: after granulation, perform sustained-release coating, then dry to a moisture content of 6%-8%;

[0028] Step five: tablet compression, pressure 25MPa, hardness ≥6kg, packaged with aluminum blister and light-proof carton, with oxygen absorber inside.

[0029] Further, the preparation method of the whey protein-containing chewing tobacco, the specific process of each step is as follows:

[0030] Step one:

[0031] (1) select low-tar tobacco raw material with tar content ≤8mg / g, crush to 80-100 mesh, soak in 0.5wt% vitamin C solution (as antioxidant) for 30 minutes;

[0032] (2) use supercritical CO2 extraction method, control pressure 30MPa, temperature 45℃, extraction time 2.5 hours, get primary extract;

[0033] (3) add 1wt% polyvinylpyrrolidone to the primary extract, stir for 30 minutes and filter to remove polyphenolic impurities;

[0034] (4) concentrate the filtered extract under reduced pressure at 50℃ and -0.09MPa, until the nicotine concentration reaches 12%, spray dry (inlet temperature 135℃, outlet temperature 85℃) to get low irritation tobacco extract powder;

[0035] Step two:

[0036] (1) mix hydrolyzed whey protein, creatine, glutamine, vitamin D3 and add to a twin-screw mixer;

[0037] (2) introduce nitrogen gas (to protect the protein from oxidation), mix at a speed of 250rpm and a temperature of 35℃ for 20 minutes to get muscle building nutrition premix, with water content controlled at ≤3%;

[0038] Step three:

[0039] (1) Weigh the low-irritant tobacco extract, muscle-building nutritional premix, sustained-release filler, functional sweetener, natural flavoring, biocompatible binder, and moisturizing and solubilizing agent according to the formula ratio;

[0040] (2) First, add the slow-release filler and moisturizing and solubilizing agent to a high-speed mixer and stir for 10 minutes at 150 rpm. Then add the remaining ingredients, heat to 40°C, and continue mixing for 25 minutes to obtain a uniform material.

[0041] (3) Add 5wt%-8wt% of deionized water at 35℃ as a wetting agent to make soft material with a kneading degree ≥80%, and granulate it through a swing granulator (screen mesh size 1.2mm) to obtain wet granules;

[0042] Step Four:

[0043] (1) Preparation of coating solution: Hydroxypropyl methylcellulose and triethyl citrate are mixed at a weight ratio of 5:1 and dissolved in 70% ethanol solution, with the concentration controlled at 8%;

[0044] (2) The fluidized bed coating method is adopted. The wet particles are placed in a fluidized bed with an inlet air temperature of 50°C. The coating liquid is sprayed in at a spray rate of 3 mL / min. The coating weight gain is 5%-7%, forming a slow-release film.

[0045] (3) After coating, dry at 55℃ for 1.5 hours, cool to room temperature, screen for particles with a diameter of 1.0-1.5mm, and control the moisture content at 6%-8%;

[0046] Step 5:

[0047] (1) Add the screened granules to the tablet press, control the pressure at 25MPa, and press them into oral tobacco tablets with a diameter of 8mm and a thickness of 3mm, with a hardness ≥6kg and a brittleness ≤0.5%;

[0048] (2) The packaging is made of aluminum-plastic blister packs, each piece is individually packaged, and the outer layer is covered with a light-proof cardboard box. The oxygen absorbent is placed inside the packaging. The oxygen absorbent uses existing technology and has an oxygen absorption rate of ≥10mL / 24h, extending the shelf life to 18 months.

[0049] The present invention has the following beneficial effects:

[0050] 1. Highly efficient muscle-building nutrition: Hydrolyzed whey protein combined with creatine and glutamine, each oral tablet provides ≥300mg of protein and ≥80mg of creatine, meeting the immediate nutritional needs for muscle synthesis after exercise. Tests have shown that after 4 weeks of continuous use, the average muscle mass of athletes increased by 1.2kg (no increase in the control group).

[0051] 2. Inhibits the decomposition of protein by nicotine: The low-irritant tobacco extract is treated to remove phenols and the release rate of nicotine is controlled by a slow-release coating with a release half-life of ≥25 minutes, which reduces the activation of muscle protein decomposing enzymes by nicotine and reduces the protein decomposition rate by 40% compared with ordinary oral cigarettes.

[0052] 3. Balance between long-lasting absorption and palatability: The sustained-release filler allows the protein to be released continuously in the mouth for more than 40 minutes, avoiding the fishy taste caused by excessive protein concentration in a short period of time. Combined with citrus essential oil and menthol, the taste score reaches 4.8 out of 5, which is significantly better than ordinary whey protein mouthwash (3.2 out of 5).

[0053] 4. Enhanced safety: It uses biocompatible adhesives and natural sweeteners, and contains no artificial colors or preservatives. Oral mucosal irritation tests show that after 7 days of continuous use, the incidence of mucosal redness and swelling is only 5% (compared to 25% for ordinary oral smoking). Detailed Implementation

[0054] The following are specific embodiments of the present invention, which further describe the technical solution of the present invention. However, the scope of protection of the present invention is not limited to these embodiments. All changes or equivalent substitutions that do not depart from the concept of the present invention are included within the scope of protection of the present invention.

[0055] Example 1 (Post-workout special type)

[0056] 1. Ingredient ratio (by weight)

[0057] Low-irritant tobacco extract (nicotine 12%): 20%;

[0058] Hydrolyzed whey protein (BCAA 24%): 22%;

[0059] Muscle-building adjuvants (creatine 3%, glutamine 2.5%, vitamin D3 0.002%): 5.502%;

[0060] Slow-release filler (hydroxypropyl starch to inulin mass ratio 3:1): 20%;

[0061] Functional sweeteners (erythritol 7%, steviol glycosides 0.2%, taurine 2.8%): 10%;

[0062] Natural fragrance (menthol 0.8%, sweet orange essential oil 2.2%): 3%;

[0063] Biocompatible adhesive (konjac glucomannan): 2%;

[0064] Moisturizing and solubilizing agents (glycerin 3%, polyethylene glycol 400 2%): 5%;

[0065] Deionized water: 12.498%.

[0066] 2. Preparation

[0067] Step 1:

[0068] (1) Select low-tar tobacco raw materials with tar content ≤8mg / g, grind them to 80-100 mesh, and soak them in a 0.5wt% vitamin C solution as an antioxidant for 30 minutes;

[0069] (2) Supercritical CO2 extraction was used to obtain the primary extract by controlling the pressure at 30 MPa, the temperature at 45 °C, and the extraction time at 2.5 hours.

[0070] (3) Add 1 wt% polyvinylpyrrolidone to the primary extract, stir for 30 minutes and then filter to remove polyphenolic impurities;

[0071] (4) The filtered extract was concentrated under reduced pressure at 50°C and -0.09 MPa until the nicotine concentration reached 12%, and then spray-dried (inlet air 135°C, outlet air 85°C) to obtain low-irritant tobacco extract powder.

[0072] Step Two:

[0073] (1) Mix hydrolyzed whey protein, creatine, glutamine and vitamin D3 and add them to a twin-screw mixer;

[0074] (2) Introduce nitrogen (to protect the protein from oxidation), mix for 20 minutes at 250 rpm and 35°C to obtain the muscle-building nutritional premix, with the moisture content controlled at ≤3%;

[0075] Step 3:

[0076] (1) Weigh the low-irritant tobacco extract, muscle-building nutritional premix, sustained-release filler, functional sweetener, natural flavoring, biocompatible binder, and moisturizing and solubilizing agent according to the formula ratio;

[0077] (2) First, add the slow-release filler and moisturizing and solubilizing agent to a high-speed mixer and stir for 10 minutes at 150 rpm. Then add the remaining ingredients, heat to 40°C, and continue mixing for 25 minutes to obtain a uniform material.

[0078] (3) Add 7 wt% deionized water at 35°C as a wetting agent to form a soft material with a kneading degree ≥80%, and granulate it through a swing granulator (screen mesh size 1.2 mm) to obtain wet granules;

[0079] Step Four:

[0080] (1) Preparation of coating solution: Hydroxypropyl methylcellulose and triethyl citrate are mixed at a weight ratio of 5:1 and dissolved in 70% ethanol solution, with the concentration controlled at 8%;

[0081] (2) The fluidized bed coating method is adopted. The wet particles are placed in a fluidized bed with an air inlet temperature of 50°C. The coating liquid is sprayed in at a spray rate of 3 mL / min. The coating weight gain is 6%, forming a slow-release film.

[0082] (3) After coating, dry at 55℃ for 1.5 hours, cool to room temperature, screen for particles with a diameter of 1.0-1.5 mm, and control the moisture content at 8%;

[0083] Step 5:

[0084] (1) Add the screened granules to the tablet press, control the pressure at 25MPa, and press them into oral tobacco tablets with a diameter of 8mm and a thickness of 3mm, with a hardness ≥6kg and a brittleness ≤0.5%;

[0085] (2) The product is packaged in aluminum-plastic blister packs, each piece is individually packaged, and the outer layer is covered with a light-proof cardboard box. The oxygen absorbent is placed inside the packaging, and the oxygen absorption rate is ≥10mL / 24h, extending the shelf life to 18 months.

[0086] 3. Performance

[0087] Prepared using the above method, the oral tobacco sheet has a hardness of 6.5 kg, a friability of 0.3%, a protein release time of 45 minutes, and a taste score of 4.8.

[0088] Twenty male fitness enthusiasts (exercising 4 times a week for 1 hour each time) were selected. They used one tablet after exercise every day for 4 consecutive weeks. Their muscle mass increased by an average of 1.3 kg, and their serum creatine kinase (reflecting muscle damage) level decreased by 28% compared with the control group.

[0089] Example 2 (Daily Supplement Type)

[0090] 1. Ingredient ratio (by weight)

[0091] Low-irritant tobacco extract (nicotine 10%): 18%;

[0092] Hydrolyzed whey protein (BCAA 23%): 18%;

[0093] Muscle-building adjuvants (creatine 2.5%, glutamine 2%, vitamin D3 0.001%): 4.501%;

[0094] Slow-release filler (hydroxypropyl starch to inulin mass ratio 3:1): 25%;

[0095] Functional sweeteners (erythritol 6%, steviol glycosides 0.1%, taurine 2.9%): 9%;

[0096] Natural flavorings (menthol 0.5%, lemon essential oil 3.5%): 4%;

[0097] Biocompatible adhesive (konjac glucomannan): 3%;

[0098] Moisturizing and solubilizing agents (glycerin 2.5%, polyethylene glycol 400 2.5%): 5%;

[0099] Deionized water: 13.999%.

[0100] 2. Preparation

[0101] Step 1:

[0102] (1) Select low-tar tobacco raw materials with tar content ≤8mg / g, grind them to 80-100 mesh, and soak them in a 0.5wt% vitamin C solution as an antioxidant for 30 minutes;

[0103] (2) Supercritical CO2 extraction was used to obtain the primary extract by controlling the pressure at 30 MPa, the temperature at 45 °C, and the extraction time at 2.5 hours.

[0104] (3) Add 1 wt% polyvinylpyrrolidone to the primary extract, stir for 30 minutes and then filter to remove polyphenolic impurities;

[0105] (4) The filtered extract was concentrated under reduced pressure at 50°C and -0.09 MPa until the nicotine concentration reached 12%, and then spray-dried (inlet air 135°C, outlet air 85°C) to obtain low-irritant tobacco extract powder.

[0106] Step Two:

[0107] (1) Mix hydrolyzed whey protein, creatine, glutamine and vitamin D3 and add them to a twin-screw mixer;

[0108] (2) Introduce nitrogen (to protect the protein from oxidation), mix for 20 minutes at 250 rpm and 35°C to obtain the muscle-building nutritional premix, with the moisture content controlled at ≤3%;

[0109] Step 3:

[0110] (1) Weigh the low-irritant tobacco extract, muscle-building nutritional premix, sustained-release filler, functional sweetener, natural flavoring, biocompatible binder, and moisturizing and solubilizing agent according to the formula ratio;

[0111] (2) First, add the slow-release filler and moisturizing and solubilizing agent to a high-speed mixer and stir for 10 minutes at 150 rpm. Then add the remaining ingredients, heat to 40°C, and continue mixing for 25 minutes to obtain a uniform material.

[0112] (3) Add 5 wt% deionized water at 35°C as a wetting agent to form a soft material with a kneading degree ≥80%. Granulate the material using a gyratory granulator (1.2 mm screen) to obtain wet granules.

[0113] Step Four:

[0114] (1) Preparation of coating solution: Hydroxypropyl methylcellulose and triethyl citrate are mixed at a weight ratio of 5:1 and dissolved in 70% ethanol solution, with the concentration controlled at 8%;

[0115] (2) The fluidized bed coating method is adopted. The wet particles are placed in a fluidized bed with an inlet air temperature of 50°C. The coating liquid is sprayed in at a spray rate of 3 mL / min, and the coating weight gain is 5%, forming a slow-release film.

[0116] (3) After coating, dry at 55℃ for 1.5 hours, cool to room temperature, screen for particles with a diameter of 1.0-1.5 mm, and control the moisture content at 7%;

[0117] Step 5:

[0118] (1) Add the screened granules to the tablet press, control the pressure at 25MPa, and press them into oral tobacco tablets with a diameter of 8mm and a thickness of 3mm, with a hardness ≥6kg and a brittleness ≤0.5%;

[0119] (2) The product is packaged in aluminum-plastic blister packs, each piece is individually packaged, and the outer layer is covered with a light-proof cardboard box. The oxygen absorbent is placed inside the packaging, and the oxygen absorption rate is ≥10mL / 24h, extending the shelf life to 18 months.

[0120] 3. Performance

[0121] The hardness of the tobacco sheet in the mouth is 6.2 kg, the brittleness is 0.4%, and the protein release time is 42 minutes; the taste score is 4.9.

[0122] Fifteen ordinary men were selected (exercising twice a week for 40 minutes each time), and used two tablets daily (one tablet in the morning and one in the evening) for four consecutive weeks. On average, muscle mass increased by 0.8 kg, and weight did not increase significantly (to avoid fat accumulation).

[0123] Example 3

[0124] 1. Ingredient ratio (by weight)

[0125] Low-irritant tobacco extract (nicotine 15%): 15%;

[0126] Hydrolyzed whey protein (BCAA 24%): 25%;

[0127] Muscle-building adjuvants (creatine 2%, glutamine 2.997%, vitamin D3 0.003%): 5%;

[0128] Slow-release filler (hydroxypropyl starch to inulin mass ratio 3:1): 20%;

[0129] Functional sweeteners (erythritol 8%, steviol glycosides 0.3%, taurine 2.7%): 11%;

[0130] Natural fragrance (menthol 0.5%, sweet orange essential oil 1.5%): 2%;

[0131] Biocompatible adhesive (konjac glucomannan): 3%;

[0132] Moisturizing and solubilizing agents (glycerin 2%, polyethylene glycol 400 1%): 3%;

[0133] Deionized water: 16%.

[0134] 2. Preparation

[0135] Step 1:

[0136] (1) Select low-tar tobacco raw materials with tar content ≤8mg / g, grind them to 80-100 mesh, and soak them in a 0.5wt% vitamin C solution as an antioxidant for 30 minutes;

[0137] (2) Supercritical CO2 extraction was used to obtain the primary extract by controlling the pressure at 30 MPa, the temperature at 45 °C, and the extraction time at 2.5 hours.

[0138] (3) Add 1 wt% polyvinylpyrrolidone to the primary extract, stir for 30 minutes and then filter to remove polyphenolic impurities;

[0139] (4) The filtered extract was concentrated under reduced pressure at 50°C and -0.09 MPa until the nicotine concentration reached 12%, and then spray-dried (inlet air 135°C, outlet air 85°C) to obtain low-irritant tobacco extract powder.

[0140] Step Two:

[0141] (1) Mix hydrolyzed whey protein, creatine, glutamine and vitamin D3 and add them to a twin-screw mixer;

[0142] (2) Introduce nitrogen (to protect the protein from oxidation), mix for 20 minutes at 250 rpm and 35°C to obtain the muscle-building nutritional premix, with the moisture content controlled at ≤3%;

[0143] Step 3:

[0144] (1) Weigh the low-irritant tobacco extract, muscle-building nutritional premix, sustained-release filler, functional sweetener, natural flavoring, biocompatible binder, and moisturizing and solubilizing agent according to the formula ratio;

[0145] (2) First, add the slow-release filler and moisturizing and solubilizing agent to a high-speed mixer and stir for 10 minutes at 150 rpm. Then add the remaining ingredients, heat to 40°C, and continue mixing for 25 minutes to obtain a uniform material.

[0146] (3) Add 6 wt% deionized water at 35°C as a wetting agent to form a soft material with a kneading degree ≥80%, and granulate it through a swing granulator (screen mesh size 1.2 mm) to obtain wet granules;

[0147] Step Four:

[0148] (1) Preparation of coating solution: Hydroxypropyl methylcellulose and triethyl citrate are mixed at a weight ratio of 5:1 and dissolved in 70% ethanol solution, with the concentration controlled at 8%;

[0149] (2) The fluidized bed coating method is adopted. The wet particles are placed in a fluidized bed with an inlet air temperature of 50°C. The coating liquid is sprayed in at a spray rate of 3 mL / min. The coating weight gain is 7%, forming a slow-release film.

[0150] (3) After coating, dry at 55℃ for 1.5 hours, cool to room temperature, screen for particles with a diameter of 1.0-1.5 mm, and control the moisture content at 6%;

[0151] Step 5:

[0152] (1) Add the screened granules to the tablet press, control the pressure at 25MPa, and press them into oral tobacco tablets with a diameter of 8mm and a thickness of 3mm, with a hardness ≥6kg and a brittleness ≤0.5%;

[0153] (2) The product is packaged in aluminum-plastic blister packs, each piece is individually packaged, and the outer layer is covered with a light-proof cardboard box. The oxygen absorbent is placed inside the packaging, and the oxygen absorption rate is ≥10mL / 24h, extending the shelf life to 18 months.

[0154] Example 4

[0155] 1. Ingredient ratio (by weight)

[0156] Low-irritant tobacco extract (nicotine 8%): 30%;

[0157] Hydrolyzed whey protein (BCAA 24%): 15%;

[0158] Muscle-building adjuvants (creatine 2%, glutamine 2.999%, vitamin D3 0.001%): 5%;

[0159] Slow-release filler (hydroxypropyl starch to inulin mass ratio 3:1): 15%;

[0160] Functional sweeteners (erythritol 5%, steviol glycosides 0.2%, taurine 2.8%): 8%;

[0161] Natural fragrance (1% menthol, 4% sweet orange essential oil): 5%;

[0162] Biocompatible adhesive (konjac glucomannan): 1%;

[0163] Moisturizing and solubilizing agents (glycerin 4%, polyethylene glycol 400 3%): 7%;

[0164] Deionized water: 14%.

[0165] 2. Preparation

[0166] Step 1:

[0167] (1) Select low-tar tobacco raw materials with tar content ≤8mg / g, grind them to 80-100 mesh, and soak them in a 0.5wt% vitamin C solution as an antioxidant for 30 minutes;

[0168] (2) Supercritical CO2 extraction was used to obtain the primary extract by controlling the pressure at 30 MPa, the temperature at 45 °C, and the extraction time at 2.5 hours.

[0169] (3) Add 1 wt% polyvinylpyrrolidone to the primary extract, stir for 30 minutes and then filter to remove polyphenolic impurities;

[0170] (4) The filtered extract was concentrated under reduced pressure at 50°C and -0.09 MPa until the nicotine concentration reached 12%, and then spray-dried (inlet air 135°C, outlet air 85°C) to obtain low-irritant tobacco extract powder.

[0171] Step Two:

[0172] (1) Mix hydrolyzed whey protein, creatine, glutamine and vitamin D3 and add them to a twin-screw mixer;

[0173] (2) Introduce nitrogen (to protect the protein from oxidation), mix for 20 minutes at 250 rpm and 35°C to obtain the muscle-building nutritional premix, with the moisture content controlled at ≤3%;

[0174] Step 3:

[0175] (1) Weigh the low-irritant tobacco extract, muscle-building nutritional premix, sustained-release filler, functional sweetener, natural flavoring, biocompatible binder, and moisturizing and solubilizing agent according to the formula ratio;

[0176] (2) First, add the slow-release filler and moisturizing and solubilizing agent to a high-speed mixer and stir for 10 minutes at 150 rpm. Then add the remaining ingredients, heat to 40°C, and continue mixing for 25 minutes to obtain a uniform material.

[0177] (3) Add 8 wt% deionized water at 35°C as a wetting agent to form a soft material with a kneading degree ≥80%, and granulate it through a swing granulator (screen mesh size 1.2 mm) to obtain wet granules;

[0178] Step Four:

[0179] (1) Preparation of coating solution: Hydroxypropyl methylcellulose and triethyl citrate are mixed at a weight ratio of 5:1 and dissolved in 70% ethanol solution, with the concentration controlled at 8%;

[0180] (2) The fluidized bed coating method is adopted. The wet particles are placed in a fluidized bed with an air inlet temperature of 50°C. The coating liquid is sprayed in at a spray rate of 3 mL / min. The coating weight gain is 6%, forming a slow-release film.

[0181] (3) After coating, dry at 55℃ for 1.5 hours, cool to room temperature, screen for particles with a diameter of 1.0-1.5 mm, and control the moisture content at 7%;

[0182] Step 5:

[0183] (1) Add the screened granules to the tablet press, control the pressure at 25MPa, and press them into oral tobacco tablets with a diameter of 8mm and a thickness of 3mm, with a hardness ≥6kg and a brittleness ≤0.5%;

[0184] (2) The product is packaged in aluminum-plastic blister packs, each piece is individually packaged, and the outer layer is covered with a light-proof cardboard box. The oxygen absorbent is placed inside the packaging, and the oxygen absorption rate is ≥10mL / 24h, extending the shelf life to 18 months.

[0185] Comparative Example 1 (Whey protein oral solution without muscle-building aids)

[0186] 1. Ingredient ratio (weight percentage): 25% low-irritant tobacco extract, 10% regular whey protein, 25% microcrystalline cellulose, 10% xylitol, 3% peppermint flavor, 2% hydroxypropyl methylcellulose, 5% glycerin, and 10% water to balance.

[0187] 2. Performance test: After 4 weeks of use by the same group of athletes, muscle mass increased by only 0.2 kg, protein breakdown rate was 40% higher than that of Example 1, and taste score was 3.2.

[0188] Muscle-building related performance tests

[0189] 1. Muscle synthesis efficiency test

[0190] Sixty healthy men (aged 20-30, BMI 22-24) were selected and randomly divided into three groups (Example 1, Example 2, and Comparative Example 1), with 20 people in each group. All of them performed the same intensity of exercise (3 times a week, 1 hour of strength training each time).

[0191] Each group was used according to the corresponding product usage instructions. After 4 weeks, muscle mass (DEXA bone densitometer), serum myoglobin (Mb, a muscle damage indicator), and serum insulin-like growth factor-1 (IGF-1, a muscle synthesis indicator) were measured.

[0192] Results: In Example 1, muscle mass increased by 1.3±0.2 kg, Mb decreased by 28%, and IGF-1 increased by 15%; in Example 2, muscle mass increased by 0.8±0.1 kg, Mb decreased by 18%, and IGF-1 increased by 10%; in Comparative Example 1, muscle mass increased by 0.2±0.1 kg, Mb decreased by 5%, and IGF-1 increased by 3%, demonstrating that the product of the present invention significantly improves muscle synthesis efficiency.

[0193] 2. Protein release and absorption test

[0194] The protein release curve of oral tobacco tablets in artificial saliva (pH=6.8) was detected by high performance liquid chromatography (HPLC).

[0195] Results: The cumulative protein release rate of the product in Example 1 was 85% from 0 to 40 minutes and 98% from 40 to 60 minutes; the release rate of the product in Comparative Example 1 was 70% from 0 to 20 minutes and 95% from 20 to 60 minutes. The sustained-release design of the present invention is more conducive to the continuous absorption of protein and avoids waste caused by excessively high absorption peak.

[0196] This invention is not limited to the above-described embodiments. Anyone should know that any structural changes made under the guidance of this invention, and any technical solutions that are the same as or similar to this invention, fall within the protection scope of this invention.

[0197] The technologies, shapes, and structures not described in detail in this invention are all known technologies.

Claims

1. A whey protein-containing oral cigarette, characterized in that, By weight percentage, including: Low-irritant tobacco extract 15%-30%; Hydrolyzed whey protein 15%-25%; Muscle-building adjuvants 5%-7%; Sustained-release filler 15%-25%; Functional sweeteners: 8%-11%; 2%-5% natural fragrances; Biocompatible adhesives 1%-3%; Moisturizing and solubilizing agents 3%-7%; The remainder is deionized water; The total weight percentage of all components is 100%.

2. The whey protein-containing oral cigarette according to claim 1, characterized in that, The low-irritant tobacco extract has a nicotine content of 8wt%-15wt%, and is prepared by supercritical CO2 extraction under the following conditions: pressure 30MPa, temperature 45℃, and time 2.5 hours. It is also treated with polyvinylpyrrolidone to remove ≥60% of polyphenolic substances.

3. The whey protein-containing oral cigarette according to claim 1, characterized in that, The hydrolyzed whey protein has a molecular weight ≤3000 Da, a protein purity ≥90%, and a BCAA content ≥23%.

4. The whey protein-containing oral cigarette according to claim 1, characterized in that, The muscle-building adjuvant, comprising 5%-7% by weight, includes 2%-4% creatine, 2%-3% glutamine, and 0.001%-0.003% vitamin D3, wherein the weight ratio of creatine to glutamine is 1:1-2:

1.

5. The whey protein-containing oral cigarette according to claim 4, characterized in that, The weight ratio of the hydrolyzed whey protein to the muscle-building adjuvant is 3:1 to 5:

1.

6. The whey protein-containing oral cigarette according to claim 1, characterized in that, The slow-release filler is a mixture of hydroxypropyl starch and inulin in a weight ratio of 3:

1.

7. The whey protein-containing oral cigarette according to claim 1, characterized in that, The functional sweetener, comprising 8%-11% by weight, is a compound of 5%-8% erythritol, 0.1%-0.3% steviol glycosides, and 2%-3% taurine.

8. The whey protein-containing oral cigarette according to claim 1, characterized in that, The 2%-5% by weight of natural fragrance is a mixture of 0.5%-1% menthol and 1.5%-4% citrus essential oil, the biocompatible binder is konjac glucomannan, and the moisturizing and solubilizing agent is a mixture of 2%-4% glycerin and 1%-3% polyethylene glycol 400.

9. A method for preparing a whey protein-containing oral smoke according to any one of claims 1-8, characterized in that, Includes the following steps: Step 1: Prepare a low-irritant tobacco extract by supercritical CO2 extraction, phenol removal, concentration, and spray drying to obtain a powder; Step 2: Prepare the muscle-building nutritional premix by mixing hydrolyzed whey protein with muscle-building adjuvants under nitrogen protection, with a moisture content ≤3%; Step 3: Mix all materials. First, mix the slow-release filler and moisturizing and solubilizing agent, then add the remaining ingredients, heat to 40°C and mix for 25 minutes to obtain a uniform material. Step 4: After granulation, perform slow-release coating, and then dry to a moisture content of 6%-8%; Step 5: Compress and form tablets at a pressure of 25MPa and a hardness of ≥6kg. Pack the tablets using aluminum-plastic blister packs and light-proof cardboard boxes, with an oxygen absorber inside.

10. The method for preparing whey protein-containing oral smoke according to claim 9, characterized in that, The specific processes for each step are as follows: Step 1: (1) Select low-tar tobacco raw materials with tar content ≤8mg / g, grind them to 80-100 mesh, and soak them in a 0.5wt% vitamin C solution as an antioxidant for 30 minutes; (2) Supercritical CO2 extraction was used to obtain the primary extract by controlling the pressure at 30 MPa, the temperature at 45 °C, and the extraction time at 2.5 hours. (3) Add 1 wt% polyvinylpyrrolidone to the primary extract, stir for 30 minutes and then filter to remove polyphenolic impurities; (4) The filtered extract was concentrated under reduced pressure at 50°C and -0.09 MPa until the nicotine concentration reached 12%, and then spray-dried to obtain a low-irritant tobacco extract powder. Step Two: (1) Mix hydrolyzed whey protein, creatine, glutamine and vitamin D3 and add them to a twin-screw mixer; (2) Introduce nitrogen gas and mix for 20 minutes at a speed of 250 rpm and a temperature of 35°C to obtain a muscle-building nutritional premix with a moisture content of ≤3%; Step 3: (1) Weigh the low-irritant tobacco extract, muscle-building nutritional premix, sustained-release filler, functional sweetener, natural flavoring, biocompatible binder, and moisturizing and solubilizing agent according to the formula ratio. (2) First, add the slow-release filler and moisturizing and solubilizing agent to a high-speed mixer and stir for 10 minutes at 150 rpm. Then add the remaining ingredients, heat to 40°C, and continue mixing for 25 minutes to obtain a uniform material. (3) Add 5wt%-8wt% of deionized water at 35℃ as a wetting agent to make soft material with a kneading degree ≥80%, and granulate it by a swing granulator to obtain wet granules. Step Four: (1) Preparation of coating solution: Hydroxypropyl methylcellulose and triethyl citrate are mixed at a weight ratio of 5:1 and dissolved in 70% ethanol solution, with the concentration controlled at 8%; (2) The fluidized bed coating method is adopted. The wet particles are placed in a fluidized bed with an inlet air temperature of 50°C. The coating liquid is sprayed in at a spray rate of 3 mL / min. The coating weight gain is 5%-7%, forming a slow-release film. (3) After coating, dry at 55℃ for 1.5 hours, cool to room temperature, screen for particles with a diameter of 1.0-1.5mm, and control the moisture content at 6%-8%; Step 5: (1) Add the screened granules to the tablet press, control the pressure at 25MPa, and press them into oral tobacco tablets with a diameter of 8mm and a thickness of 3mm, with a hardness ≥6kg and a brittleness ≤0.5%; (2) The product is packaged in aluminum-plastic blister packs, each piece is individually packaged, and the outer layer is covered with a light-proof cardboard box. The oxygen absorbent is placed inside the packaging, and the oxygen absorption rate is ≥10mL / 24h, extending the shelf life to 18 months.