Hair care composition with effects of controlling oil, preventing hair loss and repairing and preparation method of hair care composition

Abstract

The invention provides a hair care composition with oil control, hair loss prevention and repair effects and a preparation method of the hair care composition, and belongs to the technical field of hair care. The hair care composition comprises functional peptides such as myristoyl pentapeptide-4, palmitoyl tripeptide-1, acetyl tetrapeptide-3, hexapeptide-3, acetyl hexapeptide-8, palmitoyl tetrapeptide-7 and the like which are synergistically combined according to a specific proportion, and glycerin, water and a penetration enhancer are supplemented to form a basic system. By optimizing peptide structure modification, adding proportion and the steps of pH control, ultrasonic treatment and collaborative assembly in the preparation process, multi-path collaborative regulation and control of hair follicle activation, scalp inflammation inhibition and keratin synthesis enhancement are realized.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of hair care compositions, and in particular to a hair care composition with oil control, hair loss prevention and damaged hair repair effects and a preparation method thereof. BACKGROUND

[0002] With the acceleration of modern social life pace and the change of environmental factors, more and more people are facing various hair problems, such as hair loss, sebum secretion disorder, dry and brittle hair, etc. These problems not only affect the appearance and image of individuals, but also bring great trouble to the quality of life. Therefore, the demand for hair care products is increasing, especially for high-efficiency products with multiple functions such as oil control, hair loss prevention and damaged hair repair.

[0003] At present, the hair care products on the market mainly include shampoo, conditioner, hair mask, serum, etc. These products mostly rely on traditional plant extracts, vitamins, minerals and other ingredients, although they can provide certain care effects, but there are still certain limitations in solving the deep-seated problems of hair health. Especially in the demand for oil control, hair loss prevention and damaged hair repair, the existing technology has not been able to provide a comprehensive and sustainable solution. With the environmental pollution, air quality decline, increase of ultraviolet radiation and increase of modern people's life pressure, more and more people are suffering from hair loss, weak hair quality and damaged hair follicles, etc. These factors make consumers more demanding on the effect and requirements of hair care products.

[0004] In recent years, with the continuous expansion of the application of bioactive ingredients in personal care products, peptides have gradually become a research hotspot in the field of hair care due to their unique biological functions and regulation functions. As small-molecule bioactive substances, peptides can play an important role at the cellular level, such as promoting blood circulation of hair follicles, regulating sebum secretion, promoting hair cell regeneration, etc. Studies have shown that certain peptide ingredients can significantly improve the health status of hair by activating hair follicle cell proliferation, promoting nutrient supply to hair follicles and repairing hair keratin structure. However, these peptide ingredients often have different mechanisms of action, and when used alone, they may only have certain effects on certain aspects of hair problems. At the same time, how to scientifically combine different peptide ingredients so that they can better achieve multiple functions such as oil control, hair loss prevention and damaged hair repair under the synergistic action of each other is still an important challenge in current research.

[0005] In addition, although the peptide component has broad application prospects in hair follicle activation, oil regulation, hair repair, etc., there are still many imperfect aspects in the composition ratio and use effect of existing hair care products. For example, existing products usually only focus on the care effect of a certain aspect, such as oil control or hair loss prevention, but often ignore the comprehensive regulation of the scalp microenvironment, resulting in that local treatment cannot form long-term and comprehensive improvement. At the same time, the health status of the scalp, the activity of the hair follicle, the regulation of sebum secretion and other factors are interwoven with each other, and how to comprehensively regulate these factors to achieve the best hair care effect is still a technical problem to be solved. SUMMARY

[0006] In view of the deficiencies in the prior art, the purpose of the present application is to provide a hair care composition with oil control, hair loss prevention and repair functions and a preparation method thereof.

[0007] A hair care composition with oil control, hair loss prevention and repair functions comprises the following components in parts by weight: Myristoyl pentapeptide-4: 0.00001-0.0001 parts; Palmitoyl tripeptide-1: 0.00002-0.0002 parts; Acetyl tetrapeptide-3: 0.00001-0.00015 parts; Acetyl hexapeptide-8: 0.00002-0.00006 parts; Palmitoyl tetrapeptide-7: 0.000004-0.00002 parts; Hexapeptide-3: 0.00001-0.0001 parts; Water: 96-98 parts; Glycerol: 0.1-0.2 parts.

[0008] The hair care composition provided by the application has oil control, hair loss prevention and repair functions. Through the synergistic compatibility of myristoyl pentapeptide-4, acetyl tetrapeptide-3, hexapeptide-3, palmitoyl tripeptide-1, acetyl hexapeptide-8 and palmitoyl tetrapeptide-7, six functional peptides, it can produce significant systemic synergistic effect in multiple biological regulation dimensions such as hair follicle activation, scalp microenvironment regulation, nerve relaxation and hair structure repair.

[0009] Specifically, in terms of regulating the physiological cycle of hair follicles, myristoyl pentapeptide-4 is a signal initiator for hair follicle regeneration, which activates the Wnt / β-catenin signaling pathway to initiate the proliferation of hair papilla cells and the directional differentiation of hair follicle stem cells; hexapeptide-3, as a functional analog of keratinocyte growth factor (KGF), promotes angiogenesis and stromal cell metabolism around the hair follicle, providing blood flow and nutrients for hair follicle cell proliferation; acetyl tetrapeptide-3 further prolongs the duration of the hair follicle in the growth phase by regulating CDK expression, forming a complete regulation path of signal activation-microenvironment support-cycle extension. The functional coupling among the three peptides builds a physiological level signal cascade and feedback enhancement system. Compared with the single peptide promotion or support in the prior art, it has a composite biological advantage in improving hair follicle activation rate, prolonging hair growth period, and promoting hair density improvement.

[0010] In terms of regulating the scalp microenvironment, the present application realizes systematic regulation through the three-dimensional path of inflammation relief, lipid balance, and barrier repair. Palmitoyl tripeptide-1, as an inflammatory mediator regulator, can effectively down-regulate the expression of pro-inflammatory signals such as IL-6 and TNF-α, and inhibit the pro-hair loss inflammatory response in seborrheic scalp; myristoyl pentapeptide-4 regulates the PPARγ pathway in sebaceous glands, reducing sebum synthesis and intervening in sebum-induced hair follicle compression from the source; acetyl tetrapeptide-3 repairs the problem of scalp leakage and dryness caused by excessive cleaning or barrier function damage by enhancing the expression of tight junction proteins such as occludin. The three peptides are functionally complementary in regulating sebum secretion, controlling inflammatory cascade reaction, and rebuilding epidermal structure, and their interdependence forms a stable microecological closed loop, which systematically breaks the functional island of traditional products that cannot control oil, anti-inflammatory, and repair at the same time.

[0011] Palmitoyl tetrapeptide-7 has significant chronic inflammation relief and antioxidant regulation ability, can inhibit the activity of IL-8 and NF-κB signal axis, reduce the persistent micro-inflammatory state caused by neurogenic stress or environmental stimulation, and help to relieve scalp sensitivity and improve the inflammatory alopecia environment, providing a low-inflammatory background for the synergistic effect of other peptides.

[0012] Acetyl hexapeptide-8 can relax the micro-tension type neuromuscular structure by competitively inhibiting the formation of SNARE complex and reducing the release of acetylcholine at the nerve endings, and has an auxiliary intervention effect on scalp barrier abnormalities and stress alopecia caused by neurogenic stimulation. Its "botulinum-like" mechanism helps to stabilize the scalp stress state, improve the comfort of sensitive scalp, and indirectly promote the physiological balance of the hair follicle area.

[0013] In terms of damaged hair repair, acetyl tetrapeptide-3 can stimulate keratin synthesis and enhance intercellular adhesion of stratum corneum, thereby improving the tension and flexibility of hair from the structural basis; hexapeptide-3 can effectively improve the dryness, breakage and frizz of hair by inducing epithelial regeneration and maintaining the moisture content of stratum corneum; and myristoyl pentapeptide-4 can reduce the oxidative damage of hair cuticle caused by ultraviolet light and environmental stress by improving local ATP energy supply and scavenging oxidative free radicals. After synergizing with the anti-stress mechanism of acetyl hexapeptide-8, the ROS level and barrier oxidation can be further reduced, thereby realizing the composite repair system of "internal stability and external protection".

[0014] The six functional peptides of the present application each undertake a non-interchangeable functional pathway in signal initiation, nutritional support, cycle regulation, inflammation inhibition, nerve relaxation and stratum corneum repair, and synergize with each other and depend on each other's action to form a multi-path molecular network with clear positioning and synergy.

[0015] Further, the weight ratio of the myristoyl pentapeptide-4, palmitoyl tripeptide-1, acetyl tetrapeptide-3 and hexapeptide-3 is 1:(0.8-1.2):(0.5-1.5):(0.8-1.2).

[0016] The present application realizes the dose synergistic control of each polypeptide functional pathway by limiting the weight ratio of myristoyl pentapeptide-4, hexapeptide-3, acetyl tetrapeptide-3 and palmitoyl tripeptide-1 to 1:(0.8-1.2):(0.5-1.5):(0.8-1.2). Among them, myristoyl pentapeptide-4, as a key factor for activating the Wnt / β-catenin pathway, is set to a ratio of 1 to ensure that it activates the hair follicle signal without causing excessive skin irritation. The ratio of hexapeptide-3 is set to 0.8-1.2, which can avoid path interference or competitive activation of the Wnt signal on the premise of fully supporting angiogenesis and nutrient supply around the hair follicle. The ratio of acetyl tetrapeptide-3 is set to 0.5-1.5, which can not only realize the extension of the hair follicle cycle and the regulation of keratin expression, but also effectively avoid the risk of barrier disorder or metabolic inhibition induced by high doses. The ratio of palmitoyl tripeptide-1 is controlled to be 0.8-1.2, which can accurately exert its biological activity in down-regulating inflammatory factors (such as TNF-α and IL-6) and repairing the barrier function, while reducing the dryness or discomfort reaction of the skin caused by excessive use.

[0017] Further, it also includes 0.001-0.005 parts by weight of silver linden extract.

[0018] Silver linden extract, as a complementary plant-derived component, enhances the overall system's antioxidant defense and microenvironment homeostasis regulation functions. Silver linden buds are rich in flavonoids, polyphenols, tannins, and natural glycosides, possessing broad-spectrum free radical scavenging capabilities. They can inhibit ROS (reactive oxygen species)-induced lipid peroxidation, protein oxidation, and DNA damage, thereby alleviating oxidative stress responses in the scalp epidermis and hair follicle tissue.

[0019] Its main mechanisms of action include: inhibiting the oxidation chain reaction, protecting the integrity of the lipid and protein structure of the stratum corneum, and maintaining the scalp barrier function; alleviating chronic oxidative damage caused by external stimuli such as ultraviolet rays, pollutants, and heat stress, and enhancing the tolerance of the hair follicle habitat; synergistically working with keratin synthesis peptides such as acetyl tetrapeptide-3 and hexapeptide-3 to stabilize the extracellular matrix environment and improve the efficiency of structural repair; and synergistically working with the anti-inflammatory effect of palmitoyl tetrapeptide-7 to achieve a closed loop of anti-inflammatory and antioxidant effects by inhibiting oxidatively activated inflammatory pathways (such as NF-κB).

[0020] The addition of silver linden extract compensates for the insufficient oxidative defense faced by relying solely on peptides to construct signal regulation and structural repair networks, thus building a complex regulatory structure supported by signaling, cycles, inflammation, neural regulation, and an antioxidant barrier. This component, acting as a "natural barrier stabilizing factor," is particularly crucial in enhancing overall tolerance and improving conditions conducive to hair loss, especially in sensitive scalp environments.

[0021] Furthermore, the pH value of the composition is 5.2 to 6.5.

[0022] The normal pH value of the scalp is usually in the slightly acidic range of 4.5 to 5.5. This environment helps maintain the integrity of the stratum corneum and the function of the sebum film, thereby inhibiting excessive microbial growth, reducing the risk of inflammation, and maintaining the skin's water-oil balance. To balance physiological adaptability and functional stability, the pH value of the hair care composition in this invention is set to 5.2 to 6.5, with the lower limit close to the natural pH value of the scalp, while the upper limit takes into account the chemical stability of peptide components and the system's buffering capacity, avoiding irritation to sensitive users caused by a highly acidic environment.

[0023] The conformational stability and solubility of peptide active substances are highly sensitive to pH. If the pH is too low, acylated peptides may undergo hydrolysis or conformational collapse, while if the pH is too high, peptide chain oxidation or inactivation may easily occur. Setting the pH range of 5.2 to 6.5 helps to ensure the ideal stability and synergistic activity output of myristoyl pentapeptide-4, acetyl tetrapeptide-3, hexapeptide-3, and palmitoyl tripeptide-1 used in this invention during use.

[0024] Furthermore, a slightly acidic environment can enhance the scalp's absorption efficiency of active ingredients. When the stratum corneum maintains a normal charge state at a suitable pH level, it is easier for short-chain peptides to penetrate the hair follicles and dermis, ensuring their physiological functions in signaling pathway activation, nutritional support, and inflammation suppression can proceed smoothly, thereby enhancing the overall oil-controlling, hair loss-preventing, and repairing effects of the composition.

[0025] Furthermore, the myristoyl pentapeptide-4 and palmitoyl tripeptide-1 are modified with C14-C16 straight-chain fatty acyl groups, and the acetyl tetrapeptide-3 is modified with N-terminal acetylation.

[0026] By precisely defining the molecular weight and structural modification methods of myristoyl pentapeptide-4, acetyl tetrapeptide-3, hexapeptide-3, and palmitoyl tripeptide-1, the physical conditions and delivery efficiency of the synergistic effect between peptides were optimized at the molecular structure level, thereby enhancing the synergistic performance of the composition in the triple mechanism of oil control, hair loss prevention, and repair. The defined molecular weight range of 400–1200 Da is at the balance point between physiological penetration and functional activity, ensuring that the peptides effectively reach the hair follicles and their surrounding microenvironment after penetrating the scalp keratin barrier, while avoiding the problems of limited absorption due to excessively large molecules or insufficient duration of action due to excessively small molecules.

[0027] Both myristoyl pentapeptide-4 and palmitoyl tripeptide-1 are modified with C14–C16 straight-chain fatty acyl groups. This modification significantly enhances the peptide's affinity for scalp lipid structures, strengthens its membrane anchoring ability and local retention time in the epidermis, and is particularly beneficial for its binding efficiency to receptors on target cell membranes. This structural advantage not only improves individual functional performance but also contributes to the synergistic distribution and orderly release of the overall peptide system, avoiding physical repulsion or pathway conflicts between components. The N-terminal acetylation modification of acetyl tetrapeptide-3 improves its conformational stability in a weakly acidic environment and slows its degradation rate under the action of local proteases, thereby ensuring that it maintains an effective concentration in the hair follicle region for a sufficiently long time to support its biological efficacy in regulating CDK expression, prolonging the hair growth phase, and promoting keratin synthesis.

[0028] Furthermore, the structural modifications of the four peptides exhibit a clear amphiphilic gradient, with hydrophobic lipopeptides dominating membrane binding and delivery within the lipid environment, while hydrophilic short peptides dominate aqueous diffusion and signal cascades. This facilitates the formation of self-assembled microstructures or liquid crystal distributions in the glycerol-water system, further enhancing the local synergistic delivery efficiency and the probability of contact with biological targets. The structural specificity also ensures that the four peptides possess clear, non-interchangeable localization along their functional pathways: myristoyl pentapeptide-4 activates the Wnt / β-catenin pathway, palmitoyl tripeptide-1 regulates inflammation-related signals, acetyl tetrapeptide-3 regulates cell cycle and keratin synthesis, and hexapeptide-3 mimics KGF to promote nutrient delivery and angiogenesis. Their respective pathways complement each other rather than interfere with each other, providing a structural basis and distributional guarantee for the effective expression of the synergistic mechanism.

[0029] Furthermore, 0.002 to 0.004 parts by weight of a penetration enhancer are added, wherein the penetration enhancer is selected from one or more of propylene glycol, hexanediol, butanediol and pentanediol.

[0030] Water, as the primary solvent for peptides, maintains a dominant proportion in the overall formulation, ensuring the full dissolution of hydrophilic peptides such as acetyl tetrapeptide-3 and hexapeptide-3. Insufficient water content can lead to peptide aggregation, adsorption, or conformational collapse due to concentration, affecting their diffusion behavior and target binding ability in the scalp environment. Conversely, excessive water content results in excessive dilution of the overall system, significantly reducing the unit concentration of peptides and affecting the maintenance of minimum effective concentrations in locally activated signaling pathways (such as Wnt / β-catenin and KGF-like pathways). Glycerin acts as a bifunctional structure modifier in this system, providing a long-lasting moisturizing environment and stabilizing peptide distribution and controlling their diffusion rate by increasing system viscosity and intermolecular interactions. Maintaining a glycerin ratio between 1 / 12 and 1 / 18 of water represents an optimal balance between physiological tolerance and functional synergy. This ratio can create a suitable biphasic microenvironment, which is conducive to the formation of dispersed but stable microcluster structures of acyl-modified peptides such as myristoyl pentapeptide-4 and palmitoyl tripeptide-1 in the system, allowing them to be released slowly to the cell membrane surface, enhancing membrane anchoring efficiency, while not causing side effects such as scalp stickiness, pore blockage, or osmotic inhibition caused by excessive glycerol.

[0031] Based on this glycerol-water system, 0.2 to 1.0 parts by weight of a penetration enhancer are further introduced to reduce the diffusion resistance of peptide molecules to the skin barrier and enhance the tissue penetration rate of tetrapeptide structures by changing the lipid arrangement structure of the stratum corneum or temporarily regulating the intercellular spaces of keratinocytes.

[0032] Furthermore, the mass ratio of the acetyl tetrapeptide-3 to hexapeptide-3 combination is 1:(1.2 to 2.5).

[0033] The synergistic relationship between acetyl tetrapeptide-3 and hexapeptide-3 is a "signal-driven, structure-supported" complementary relationship. An imbalance in their ratio directly affects the integrity of the keratin synthesis chain. If the hexapeptide-3 concentration is insufficient, even if acetyl tetrapeptide-3 effectively activates KRT gene expression, insufficient cellular metabolic support limits protein translation and folding efficiency, ultimately hindering the formation of an effective keratin structural protein network. Conversely, if the acetyl tetrapeptide-3 concentration is too low, even with sufficient nutrient supply, insufficient keratin-encoding activation signal strength will also prevent the achievement of repair goals. Setting their ratio to 1:(1.2–2.5) ensures that hexapeptide-3 has a moderate relative lead over acetyl tetrapeptide-3, guaranteeing stable and sufficient structural transformation support for the protein production pathway after signal input. This represents an engineered regulation of the "resource-instruction matching" in the repair pathway.

[0034] A method for preparing a hair care composition with oil-controlling, anti-hair loss, and repairing effects includes the following steps: S1: Weigh out myristoyl pentapeptide-4, palmitoyl tripeptide-1, acetyl tetrapeptide-3, hexapeptide-3 acetyl hexapeptide-8 and palmitoyl tetrapeptide-7, and mix them according to the weight proportions described in the formula; S2: Mix the above peptide components with glycerin in a predetermined ratio, stir until completely homogeneous, then heat to 40-50℃, maintain this temperature range and stir for 10-20 minutes until all components are completely dissolved and a transparent homogeneous solution is formed; S3: Add water to the above solution at a rate of 5-10 mL per minute and continue stirring until a uniformly dispersed solution is obtained. The stirring speed should be controlled at 100-200 rpm. S4: Adjust the pH of the solution to 5.2-6.5 to ensure that all components are under ideal self-assembly conditions; S5: The above solution is subjected to ultrasonic treatment at a frequency of 20-40kHz for 5-10 minutes. S6: Cool the above mixture to 20-25°C and remove moisture under vacuum conditions to obtain the hair care composition with oil control, anti-hair loss and repair effects.

[0035] In steps S1 and S2, the peptide components are first premixed with glycerol at low temperature and then fully dissolved under constant temperature conditions of 40–50°C. This ensures the dispersibility of acylated peptides such as palmitoyl tripeptide-1 and myristoyl pentapeptide-4, preventing their aggregation or precipitation at low temperatures. It also prevents conformational inactivation of the peptide chains due to high temperatures. The addition of glycerol not only serves as a dissolution medium but also acts as an initial distribution buffer between peptides, inhibiting the mutual repulsion between hydrophilic and hydrophobic peptides and providing preliminary stability conditions for subsequent synergistic structure construction.

[0036] In step S3, water is slowly added at a rate of 5-10 mL / min, while the stirring rate is controlled at 100-200 rpm. This effectively avoids conformational collapse or destruction of weak aggregate states caused by sudden dilution or high shearing between peptides, allowing for the initial formation of self-assembled primary structures such as micelles, linear associations, or liquid crystal microregions. The dispersion system formed in this stage retains the independent biological functions of the peptide components and also possesses sufficient spatial synergistic potential.

[0037] In step S4, the pH value is adjusted to between 5.2 and 6.5, corresponding to the pH range in claim 4. This range represents the optimal balance between maintaining stable peptide conformation, activation capacity, and penetration efficiency. This pH setting can both inhibit excessive ionization of carboxyl or amino groups in the peptide chain, preventing structural changes, and activate the optimal reaction range of hair follicle cells and sebaceous gland-related receptors, ensuring the bioreactivity efficiency of the formulation.

[0038] Step S5, ultrasonic treatment, further breaks down any potential peptide aggregates, improves particle size uniformity, and makes the synergistic structure more stable. An ultrasonic frequency of 20-40 kHz and a treatment time of 5-10 minutes can promote the rapid formation of micelles or micro-aggregates without damaging the active peptides, allowing the four peptides to be distributed in a synergistic conformation within the aqueous system, providing particle size-level assurance for subsequent absorption and targeting.

[0039] Step S6 involves cooling to 20-25°C and concentrating under vacuum, ultimately completing the structural solidification process of the composition. This step helps to lock in the synergistic structure of the peptides and avoids peptide degradation and micellar deconstruction caused by high-temperature concentration through gentle dehydration. This step is the final "closing step" for the stable expression of the synergistic mechanism, ensuring that the composition has good storage stability and reproducibility of synergistic function before use.

[0040] Furthermore, following step S3, the following optimization steps are also included: Optimization step 1: Let the homogeneous solution containing polypeptide, glycerol and water stand at a constant temperature of 25-30°C for 60-120 minutes; Optimization step 2: Subsequently, after the settling period is completed, pH adjustment is performed. The pH adjustment step is performed under the condition that the stirring speed does not exceed 100 rpm, and the adjustment speed is controlled to be 0.05 to 0.1 pH units per minute.

[0041] By introducing a isothermal settling step (optimized step 1) after dilution and stirring in the aqueous phase to form a preliminary homogeneous dispersion, and then adjusting the pH in a low-shear, slow manner after settling (optimized step 2), a crucial structural buffering process is provided for the establishment of synergistic conformations between peptides and physiological compatibility between pathways. These two optimized steps are not simple "sedimentation" or "direct pH adjustment" in conventional solution preparation operations, but rather a purposeful pre-construction process of synergistic structures, which is a necessary condition for the four functional peptides in this invention to achieve synergistic effects at the microscale.

[0042] In optimization step 1, the mixed solution containing peptides, glycerol, and water is allowed to stand at a constant temperature of 25–30°C for 60–120 minutes to initiate a primary conformational integration process within the system. Myristoyl pentapeptide-4 and palmitoyl tripeptide-1 are acyl-modified lipopeptides with a certain tendency for hydrophobic aggregation, while hexapeptide-3 and acetyl tetrapeptide-3 are hydrophilic peptides exhibiting high-speed migration and diffusion behavior in solution. If pH adjustment or further treatment is performed directly without the standing process, this difference in molecular migration speed will lead to uneven distribution and structural interference within the system, disrupting the independent spatial localization and subsequent delivery sequence of each peptide. Gentle standing promotes weak interactions (such as hydrophobic association, hydrogen bond complementarity, and electrostatic pairing) between structurally compatible peptides, forming a co-conformation precursor with controllable particle size and spatial compatibility. This results in a composition with good particle size controllability and uniform distribution, laying the microstructural foundation for the controllable expression of the co-conformation mechanism.

[0043] Step 2 of the optimization further requires that pH adjustment be carried out at a low stirring rate (≤100 rpm) and a slow rate of change (0.05–0.1 pH units / min). This operation avoids conformational mutations or self-assembly structure disruptions in peptides caused by drastic pH changes. Peptide molecules are sensitive to changes in charge distribution and spatial folding under different pH conditions. If the pH adjustment process is too rapid, it can easily cause a shift in the critical point, leading to precipitation or inactivation of some peptides. Slow adjustment allows the system to maintain a quasi-steady-state microenvironment, ensuring that peptide molecules maintain their cooperative spatial arrangement as they gradually reach their optimal conformation. This avoids arbitrary decoupling or delayed activation of the four pathways of "signal-nutrient-cycle-barrier" in the cooperative mechanism.

[0044] Furthermore, following the S5 ultrasonic processing step, the following optimization steps are also included: Optimization step 3: The ultrasonically treated solution is allowed to stand at 20-25℃ for 30-60 minutes to form a stable self-assembled micelle structure with a particle size controlled between 30-100nm and a particle size distribution index not higher than 0.25. Optimization step 4: Sterilization filtration is performed through a microporous membrane with a pore size of 0.22μm to remove large molecular aggregates that are not in a micelle state.

[0045] In optimization step 3, the ultrasonically treated solution is allowed to stand at 20–25°C for 30–60 minutes. This essentially provides a buffer period for the peptide microstructure system, which has just undergone mechanical disturbance, allowing it to naturally transform from a state of intense ultrasound-induced aggregation to a stable, low-energy, and uniformly distributed self-assembled conformation. During this process, weak interactions between peptides (including hydrophobic interactions, van der Waals forces, and dipole-dipole pairing) are reconstructed. In particular, the spatial perturbations caused by ultrasound between acylated structures (such as myristoyl pentapeptide-4 and palmitoyl tripeptide-1) and hydrophilic peptides (such as acetyl tetrapeptide-3 and hexapeptide-3) are restored, resulting in the formation of micromicelles or linearly associated particles with a particle size controlled at 30–100 nm and a PDI value below 0.25. This microparticle structure not only possesses stable release capabilities but also exhibits good permeability and tissue penetration, supporting the continuous expression of the four functional peptides in the three pathways of oil control, hair loss prevention, and repair.

[0046] Step 4, optimizing the filtration process using a 0.22μm microporous membrane for sterilization, is a structural purification and safety enhancement operation based on structural stability. This step not only shields against microbial contaminants but, more importantly, removes non-uniform macromolecular aggregates or free components that may form during preparation, ensuring that all active ingredients in the final composition exist in a synergistic state. This purification process can be considered a "synergistic purification process" at a functional level because it directly affects the simultaneous absorption and complementary functions of the four peptides at different tissue layers of the scalp. If heterogeneous particles larger than 200nm still exist in the system, it can easily lead to: First, the penetration is delayed or uneven, disrupting the timing of the coordinated path. Secondly, local retention or accumulation can cause skin discomfort or even induce inflammation; Third, it affects peptide delivery efficiency and spatial interaction probability, diluting the overall biological effects of the composition.

[0047] Therefore, through this particle size re-control and impurity removal step, the final product not only possesses good physicochemical stability, but also ensures that it expresses the hair follicle initiation function of myristoyl pentapeptide-4, the nutrient supply function of hexapeptide-3, the cycle regulation function of acetyl tetrapeptide-3, and the inflammation regulation function of palmitoyl tripeptide-1 in the optimal structural state during biological action. This is the core supporting condition for the synergistic mechanism to achieve "synchronous action, balanced delivery, and non-interference of pathways".

[0048] Compared with the prior art, the beneficial effects of the present invention are mainly reflected in the following aspects: This invention systematically defines the formulation parameters and preparation process required for the ratio, structural modification, addition amount, and synergistic mechanism of four functional peptides, constructing a hair care system with a clear action pathway, balanced signal intensity, and stable synergistic structure for oil control, hair loss prevention, and repair. By precisely controlling the mass ratio between peptides and the additive environment (such as pH, penetration enhancer, glycerol-water distribution, etc.), it ensures that each functional peptide is synergistically expressed and does not interfere with each other in different physiological pathways such as hair follicle activation, keratin expression, inflammation suppression, and nutrient supply. At the same time, through key preparation steps such as low-shear static incubation, slow pH adjustment, ultrasonic particle size control, and microporous filtration, its microscopic synergistic conformation and delivery structure are further stabilized, improving bioavailability and consistency of action. Attached Figure Description

[0049] Figure 1 Hair follicle images of the mouse hair follicle elongation test group (Example 1), control group, and positive group on day 1 and day 14. Detailed Implementation

[0050] The present invention will now be described in detail with reference to the embodiments and accompanying drawings.

[0051] The peptide components used in this invention—myristoyl pentapeptide-4, palmitoyl tripeptide-1, acetyl tetrapeptide-3, acetyl hexapeptide-8, palmitoyl tetrapeptide-7, and hexapeptide-3—are all produced and supplied by Dongguan Weiqi Technology Co., Ltd.

[0052] Example 1 This embodiment discloses a hair care composition with oil-controlling, anti-hair loss, and repairing effects, comprising the following components in parts by weight: Myristoyl pentapeptide-4: 0.00001 parts; Palmitoyl tripeptide-1: 0.00002 parts; Acetyl tetrapeptide-3: 0.00015 parts; Hexapeptide-3: 0.0001 parts; Acetyl hexapeptide-8: 0.00002 parts; Palmitoyl tetrapeptide-7: 0.000004 parts; Water: 96 parts; Glycerin: 0.1 parts.

[0053] This embodiment also discloses a method for preparing the above-mentioned hair care composition with oil control, anti-hair loss, and repair functions, including the following steps: S1: Weigh out myristoyl pentapeptide-4, palmitoyl tripeptide-1, acetyl tetrapeptide-3, hexapeptide-3, acetyl hexapeptide-8 and palmitoyl tetrapeptide-7, and mix them according to the weight proportions described in the formula; S2: Mix the above peptide components with glycerin in a predetermined ratio, stir until completely homogeneous, then heat to 40°C, maintain this temperature range and stir for 10 minutes until all components are completely dissolved and a transparent homogeneous solution is formed; S3: Add water to the above solution at a rate of 5 mL per minute and continue stirring until a uniformly dispersed solution is obtained. The stirring speed is controlled at 100 rpm. S4: Adjust the pH of the solution to 5.2 dropwise using 0.1 mol / L citric acid solution or 0.1 mol / L triethanolamine aqueous solution, and monitor the pH in real time with a pH meter to ensure that the system is stable in a weakly acidic range and that each component is under ideal self-assembly conditions; S5: The above solution is subjected to ultrasonic treatment at a frequency of 20kHz for 5 minutes. S6: Cool the above mixture to 20°C and remove moisture under vacuum conditions to obtain the hair care composition with oil control, anti-hair loss and repair effects.

[0054] Example 2 This embodiment discloses a hair care composition with oil-controlling, anti-hair loss, and repairing effects, comprising the following components in parts by weight: Myristoyl pentapeptide-4: 0.0001 parts; Palmitoyl tripeptide-1: 0.0002 parts; Acetyl tetrapeptide-3: 0.00001 parts; Hexapeptide-3: 0.00001 parts; Acetyl hexapeptide-8: 0.00004 parts; Palmitoyl tetrapeptide-7: 0.00001 parts; Water: 98 portions; Glycerin: 0.2 parts.

[0055] This embodiment also discloses a method for preparing the above-mentioned hair care composition with oil control, anti-hair loss, and repair functions, including the following steps: S1: Weigh out myristoyl pentapeptide-4, palmitoyl tripeptide-1, acetyl tetrapeptide-3, hexapeptide-3, acetyl hexapeptide-8 and palmitoyl tetrapeptide-7, and mix them according to the weight proportions described in the formula; S2: Mix the above peptide components with glycerin in a predetermined ratio, stir until completely homogeneous, then heat to 50°C, maintain this temperature range and stir for 20 minutes until all components are completely dissolved and a transparent homogeneous solution is formed; S3: Add water to the above solution at a rate of 10 mL per minute and continue stirring until a uniformly dispersed solution is obtained. The stirring speed is controlled at 200 rpm. S4: Adjust the pH of the solution to 6.5 dropwise using 0.1 mol / L citric acid solution or 0.1 mol / L triethanolamine aqueous solution, and monitor the pH in real time with a pH meter to ensure that the system is stable in a weakly acidic range and that each component is under ideal self-assembly conditions; S5: The above solution is subjected to ultrasonic treatment at a frequency of 40 kHz for 10 minutes. S6: Cool the above mixture to 25°C and remove moisture under vacuum conditions to obtain the hair care composition with oil control, anti-hair loss and repair effects.

[0056] Example 3 This embodiment discloses a hair care composition with oil-controlling, anti-hair loss, and repairing effects, comprising the following components in parts by weight: Myristoyl pentapeptide-4: 0.00006 parts; Palmitoyl tripeptide-1: 0.00013 parts; Acetyl tetrapeptide-3: 0.00007 parts; Hexapeptide-3: 0.00006 parts; Acetyl hexapeptide-8: 0.00006 parts; Palmitoyl tetrapeptide-7: 0.00002 parts; Water: 97 parts; Glycerin: 0.2 parts.

[0057] This embodiment also discloses a method for preparing the above-mentioned hair care composition with oil control, anti-hair loss, and repair functions, including the following steps: S1: Weigh out myristoyl pentapeptide-4, palmitoyl tripeptide-1, acetyl tetrapeptide-3, hexapeptide-3, acetyl hexapeptide-8 and palmitoyl tetrapeptide-7, and mix them according to the weight proportions described in the formula; S2: Mix the above peptide components with glycerin in a predetermined ratio, stir until completely homogeneous, then heat to 45°C, maintain this temperature range and stir for 15 minutes until all components are completely dissolved and a transparent homogeneous solution is formed; S3: Add water to the above solution at a rate of 7 mL per minute and continue stirring until a uniformly dispersed solution is obtained. The stirring speed is controlled at 150 rpm. S4: Adjust the pH of the solution to 5.8 dropwise using 0.1 mol / L citric acid solution or 0.1 mol / L triethanolamine aqueous solution, and monitor the pH in real time with a pH meter to ensure that the system is stable in a weakly acidic range and that each component is under ideal self-assembly conditions; S5: The above solution is subjected to ultrasonic treatment at a frequency of 30 kHz for 70 minutes. S6: Cool the above mixture to 23°C and remove moisture under vacuum conditions to obtain the hair care composition with oil control, anti-hair loss and repair effects.

[0058] Example 4 The difference between this embodiment and Example 1 is that the weight ratio of myristoyl pentapeptide-4, palmitoyl tripeptide-1, acetyl tetrapeptide-3, and hexapeptide-3 satisfies 1:(0.8~1.2):(0.5~1.5):(0.8~1.2), specifically including the following components in parts by weight: Myristoyl pentapeptide-4: 0.00004 parts; Palmitoyl tripeptide-1: 0.00004 parts; Acetyl tetrapeptide-3: 0.00003 parts; Hexapeptide-3: 0.000035 parts; Acetyl hexapeptide-8: 0.00006 parts; Palmitoyl tetrapeptide-7: 0.00002 parts; Water: 98 portions; Glycerin: 0.2 parts.

[0059] Example 5 The difference between this embodiment and Embodiment 4 is that the mass ratio of acetyl tetrapeptide-3 to hexapeptide-3 still satisfies 1:(1.2~2.5), specifically including the following components in parts by weight: Myristoyl pentapeptide-4: 0.00005 parts; Palmitoyl tripeptide-1: 0.00006 parts; Acetyl tetrapeptide-3: 0.00003 parts; Hexapeptide-3: 0.000045 parts; Acetyl hexapeptide-8: 0.00006 parts; Palmitoyl tetrapeptide-7: 0.00002 parts; Water: 98 portions; Glycerin: 0.1 parts.

[0060] Example 6 The difference between this embodiment and Embodiment 5 is that the hair care composition also includes silver linden extract.

[0061] A hair care composition with oil-controlling, anti-hair loss, and repairing effects comprises the following components in parts by weight: Myristoyl pentapeptide-4: 0.00005 parts; Palmitoyl tripeptide-1: 0.00006 parts; Acetyl tetrapeptide-3: 0.00003 parts; Hexapeptide-3: 0.000045 parts; Acetyl hexapeptide-8: 0.00006 parts; Palmitoyl tetrapeptide-7: 0.00002 parts; Silver Linden Extract: 0.003 parts; Water: 98 portions; Glycerin: 0.1 parts.

[0062] This embodiment also discloses a method for preparing the above-mentioned hair care composition with oil control, anti-hair loss, and repair functions, including the following steps: S1: Weigh out myristoyl pentapeptide-4, palmitoyl tripeptide-1, acetyl tetrapeptide-3, hexapeptide-3, acetyl hexapeptide-8, palmitoyl tetrapeptide-7 and silver linden extract, and mix them according to the weight proportions described in the formula; S2: Mix the above peptide components with glycerin in a predetermined ratio, stir until completely homogeneous, then heat to 45°C, maintain this temperature range and stir for 15 minutes until all components are completely dissolved and a transparent homogeneous solution is formed; S3: Add water to the above solution at a rate of 7 mL per minute and continue stirring until a uniformly dispersed solution is obtained. The stirring speed is controlled at 150 rpm. S4: Adjust the pH of the solution to 5.8 dropwise using 0.1 mol / L citric acid solution or 0.1 mol / L triethanolamine aqueous solution, and monitor the pH in real time with a pH meter to ensure that the system is stable in a weakly acidic range and that each component is under ideal self-assembly conditions; S5: The above solution is subjected to ultrasonic treatment at a frequency of 30 kHz for 70 minutes. S6: Cool the above mixture to 23°C and remove moisture under vacuum conditions to obtain the hair care composition with oil control, anti-hair loss and repair effects.

[0063] Example 7 The difference between this embodiment and Example 6 is that myristoyl pentapeptide-4 and palmitoyl tripeptide-1 are modified with C14-C16 straight-chain fatty acyl groups, and acetyl tetrapeptide-3 is modified with N-terminal acetylation.

[0064] Specifically, the modification steps are as follows: 1. Fatty acylation modification of myristoyl pentapeptide-4: The pentapeptide sequence (Lys-Thr-Thr-Lys-Ser) was reacted with myristoyl chloride (C14 fatty acyl chloride) in anhydrous dichloromethane, using N,N-diisopropylethylamine as a base. Myristoyl chloride was slowly added dropwise at 0–5 °C, and the reaction was carried out at room temperature for 12 hours to form a stable amide bond structure at the N-terminus. After the reaction was completed, the reaction solution was concentrated, extracted with ethyl acetate, and purified by silica gel column chromatography to obtain N-myristoyl pentapeptide-4.

[0065] 2. Fatty acylation modification of palmitoyl tripeptide-1: Using the same process as described above, the tripeptide sequence (Gly-His-Lys) was reacted with palmitoyl chloride (C16 fatty acyl chloride) to introduce a C16 fatty acyl group at the N-terminus, forming N-palmitoyl tripeptide-1. The reaction was controlled at pH around 8.0 to maintain mild conditions and avoid peptide degradation.

[0066] 3. N-terminal acetylation modification of acetyl tetrapeptide-3: The tetrapeptide sequence (Gly-Gln-Pro-Arg) was dissolved in 0.1 mol / L phosphate buffer (PBS, pH 7.4), and acetic anhydride (Ac2O) was added as an acetylation reagent. The mixture was slowly added dropwise and stirred for 1 hour. After the acetylation reaction was completed, residual acetic anhydride was removed by dialysis using a dialysis bag (molecular weight cutoff of 300 Da), and the product was freeze-dried to obtain N-acetyl tetrapeptide-3 (Acetyl-Tetrapeptide-3).

[0067] Example 8 The difference between this embodiment and Embodiment 6 is that 0.5 parts by weight of a penetration enhancer, namely propylene glycol, are added to the hair care composition with oil control, anti-hair loss and repair effects.

[0068] This embodiment also discloses a method for preparing the above-mentioned hair care composition with oil control, anti-hair loss, and repair functions, including the following steps: S1: Weigh out myristoyl pentapeptide-4, palmitoyl tripeptide-1, acetyl tetrapeptide-3, and hexapeptide-3, and mix them according to the weight proportions stated in the formula; S2: Mix the above peptide components with glycerin and propylene glycol in a predetermined ratio, stir until completely homogeneous, then heat to 45°C, maintain this temperature range and stir for 15 minutes until all components are completely dissolved and a transparent homogeneous solution is formed. S3: Add water to the above solution at a rate of 7 mL per minute and continue stirring until a uniformly dispersed solution is obtained. The stirring speed is controlled at 150 rpm. S4: Adjust the pH of the solution to 5.8 dropwise using 0.1 mol / L citric acid solution or 0.1 mol / L triethanolamine aqueous solution, and monitor the pH in real time with a pH meter to ensure that the system is stable in a weakly acidic range and that each component is under ideal self-assembly conditions; S5: The above solution is subjected to ultrasonic treatment at a frequency of 30 kHz for 70 minutes. S6: Cool the above mixture to 23°C and remove moisture under vacuum conditions to obtain the hair care composition with oil control, anti-hair loss and repair effects.

[0069] Example 9 The difference between this embodiment and embodiment 8 is that, after step S3, the following optimization steps are also included: Optimization step 1: Let the homogeneous solution containing peptides, glycerol and water stand at a constant temperature of 30°C for 100 minutes; Optimization step 2: After the settling period is completed, pH adjustment is performed. The pH adjustment is carried out at a stirring speed of 80 rpm, and the adjustment speed is controlled at 0.05 to 0.1 pH units per minute.

[0070] Example 10 The difference between this embodiment and Embodiment 9 is that, after the S5 ultrasonic processing step, the following optimization step is also included: Optimization step 3: The ultrasonically treated solution was allowed to stand at 25°C for 60 minutes to form a stable self-assembled micelle structure with a particle size controlled at 100 nm and a particle size distribution index not higher than 0.25. Optimization step 4: Sterilization filtration is performed through a microporous membrane with a pore size of 0.22μm to remove large molecular aggregates that are not in a micelle state.

[0071] Comparative Example 1 The difference between this comparative example and Example 1 is that the hair care composition disclosed in this comparative example comprises the following components in parts by weight: Palmitoyl tripeptide-1: 0.00002 parts; Acetyl tetrapeptide-3: 0.00015 parts; Hexapeptide-3: 0.0001 parts; Water: 98 portions; Glycerin: 0.2 parts.

[0072] This embodiment also discloses a method for preparing the above-mentioned hair care composition with oil control, anti-hair loss, and repair functions, including the following steps: S1: Weigh out palmitoyl tripeptide-1, acetyl tetrapeptide-3, and hexapeptide-3, and mix them according to the weight proportions stated in the formula; S2: Mix the above peptide components with glycerin in a predetermined ratio, stir until completely homogeneous, then heat to 40°C, maintain this temperature range and stir for 10 minutes until all components are completely dissolved and a transparent homogeneous solution is formed; S3: Add water to the above solution at a rate of 5 mL per minute and continue stirring until a uniformly dispersed solution is obtained. The stirring speed is controlled at 100 rpm. S4: Adjust the pH of the solution to 5.2 dropwise using 0.1 mol / L citric acid solution or 0.1 mol / L triethanolamine aqueous solution, and monitor the pH in real time with a pH meter to ensure that the system is stable in a weakly acidic range and that each component is under ideal self-assembly conditions; S5: The above solution is subjected to ultrasonic treatment at a frequency of 20kHz for 5 minutes. S6: Cool the above mixture to 20°C and remove moisture under vacuum conditions to obtain the hair care composition with oil control, anti-hair loss and repair effects.

[0073] Comparative Example 2 The difference between this comparative example and Example 1 is that the hair care composition disclosed in this comparative example comprises the following components in parts by weight: Myristoyl pentapeptide-4: 0.0001 parts; Acetyl tetrapeptide-3: 0.00015 parts; Hexapeptide-3: 0.0001 parts; Water: 98 portions; Glycerin: 0.2 parts.

[0074] This embodiment also discloses a method for preparing the above-mentioned hair care composition with oil control, anti-hair loss, and repair functions, including the following steps: S1: Weigh out myristoyl pentapeptide-4, acetyl tetrapeptide-3, and hexapeptide-3, and mix them according to the weight proportions stated in the formula; S2: Mix the above peptide components with glycerin in a predetermined ratio, stir until completely homogeneous, then heat to 40°C, maintain this temperature range and stir for 10 minutes until all components are completely dissolved and a transparent homogeneous solution is formed; S3: Add water to the above solution at a rate of 5 mL per minute and continue stirring until a uniformly dispersed solution is obtained. The stirring speed is controlled at 100 rpm. S4: Adjust the pH of the solution to 5.2 dropwise using 0.1 mol / L citric acid solution or 0.1 mol / L triethanolamine aqueous solution, and monitor the pH in real time with a pH meter to ensure that the system is stable in a weakly acidic range and that each component is under ideal self-assembly conditions; S5: The above solution is subjected to ultrasonic treatment at a frequency of 20kHz for 5 minutes. S6: Cool the above mixture to 20°C and remove moisture under vacuum conditions to obtain the hair care composition with oil control, anti-hair loss and repair effects.

[0075] Comparative Example 3 The difference between this comparative example and Example 1 is that the hair care composition disclosed in this comparative example comprises the following components in parts by weight: Myristoyl pentapeptide-4: 0.0001 parts; Palmitoyl tripeptide-1: 0.00002 parts; Hexapeptide-3: 0.0001 parts; Water: 98 portions; Glycerin: 0.2 parts.

[0076] This embodiment also discloses a method for preparing the above-mentioned hair care composition with oil control, anti-hair loss, and repair functions, including the following steps: S1: Weigh out myristoyl pentapeptide-4, palmitoyl tripeptide-1, and hexapeptide-3, and mix them according to the weight proportions stated in the formula; S2: Mix the above peptide components with glycerin in a predetermined ratio, stir until completely homogeneous, then heat to 40°C, maintain this temperature range and stir for 10 minutes until all components are completely dissolved and a transparent homogeneous solution is formed; S3: Add water to the above solution at a rate of 5 mL per minute and continue stirring until a uniformly dispersed solution is obtained. The stirring speed is controlled at 100 rpm. S4: Adjust the pH of the solution to 5.2 dropwise using 0.1 mol / L citric acid solution or 0.1 mol / L triethanolamine aqueous solution, and monitor the pH in real time with a pH meter to ensure that the system is stable in a weakly acidic range and that each component is under ideal self-assembly conditions; S5: The above solution is subjected to ultrasonic treatment at a frequency of 20kHz for 5 minutes. S6: Cool the above mixture to 20°C and remove moisture under vacuum conditions to obtain the hair care composition with oil control, anti-hair loss and repair effects.

[0077] Comparative Example 4 The difference between this comparative example and Example 1 is that the hair care composition disclosed in this comparative example comprises the following components in parts by weight: Myristoyl pentapeptide-4: 0.0001 parts; Palmitoyl tripeptide-1: 0.00002 parts; Acetyl tetrapeptide-3: 0.00015 parts; Water: 98 portions; Glycerin: 0.2 parts.

[0078] This embodiment also discloses a method for preparing the above-mentioned hair care composition with oil control, anti-hair loss, and repair functions, including the following steps: S1: Weigh out myristoyl pentapeptide-4, palmitoyl tripeptide-1, and acetyl tetrapeptide-3, and mix them according to the weight proportions stated in the formula; S2: Mix the above peptide components with glycerin in a predetermined ratio, stir until completely homogeneous, then heat to 40°C, maintain this temperature range and stir for 10 minutes until all components are completely dissolved and a transparent homogeneous solution is formed; S3: Add water to the above solution at a rate of 5 mL per minute and continue stirring until a uniformly dispersed solution is obtained. The stirring speed is controlled at 100 rpm. S4: Adjust the pH of the solution to 5.2 dropwise using 0.1 mol / L citric acid solution or 0.1 mol / L triethanolamine aqueous solution, and monitor the pH in real time with a pH meter to ensure that the system is stable in a weakly acidic range and that each component is under ideal self-assembly conditions; S5: The above solution is subjected to ultrasonic treatment at a frequency of 20kHz for 5 minutes. S6: Cool the above mixture to 20°C and remove moisture under vacuum conditions to obtain the hair care composition with oil control, anti-hair loss and repair effects.

[0079] Comparative Example 5 This embodiment discloses a hair care composition with oil-controlling, anti-hair loss, and repairing effects, comprising the following components in parts by weight: Myristoyl pentapeptide-4: 0.00001 parts; Palmitoyl tripeptide-1: 0.00002 parts; Acetyl tetrapeptide-3: 0.00015 parts; Hexapeptide-3: 0.0001 parts; Water: 98 portions; Glycerin: 0.2 parts.

[0080] This embodiment also discloses a method for preparing the above-mentioned hair care composition with oil control, anti-hair loss, and repair functions, including the following steps: S1: Weigh out myristoyl pentapeptide-4, palmitoyl tripeptide-1, acetyl tetrapeptide-3, and hexapeptide-3, and mix them according to the weight proportions stated in the formula; S2: Mix the above peptide components with glycerin in a predetermined ratio, stir until completely homogeneous, then heat to 40°C, maintain this temperature range and stir for 10 minutes until all components are completely dissolved and a transparent homogeneous solution is formed; S3: Add water to the above solution at a rate of 5 mL per minute and continue stirring until a uniformly dispersed solution is obtained. The stirring speed is controlled at 100 rpm. S4: Adjust the pH of the solution to 5.2 dropwise using 0.1 mol / L citric acid solution or 0.1 mol / L triethanolamine aqueous solution, and monitor the pH in real time with a pH meter to ensure that the system is stable in a weakly acidic range and that each component is under ideal self-assembly conditions; S5: The above solution is subjected to ultrasonic treatment at a frequency of 20kHz for 5 minutes. S6: Cool the above mixture to 20°C and remove moisture under vacuum conditions to obtain the hair care composition with oil control, anti-hair loss and repair effects.

[0081] Detection methods 1. Mouse hair follicle elongation test 1. Experimental materials: 1.1 Mouse whisker hair follicles 1.2 Culture medium: Wiliams' E serum-free medium (Gibco, Lot: 2043416) containing 2 mL glutamine, 10 μg / mL bovine insulin, 10 ng / mL hydrocortisone, 100 U / mL penicillin, and 100 μg / mL streptomycin. 1.3 Control group: Williams' E serum-free culture medium; 1.4 Positive group: 10mM minoxidil solution (Minoxidil), minoxidil liniment, Sichuan Meida Kanghua Pharmaceutical Co., Ltd.; 1.5 Sample Group: Weigh 0.0032 g of the sample (hair care compositions of Examples 1-9 and Comparative Examples 1-5), add 10 mL of ultrapure water, mix well, prepare a 0.3 mg / mL stock solution, filter, and dilute to a concentration of 0.0006 mg / mL using Williams E serum-free medium. 2. Experimental Procedure: 2.1 Hair follicle separation: The whiskers of the mice were cut off bilaterally. The upper lip was disinfected three times with 75% ethanol. The whisker pads were cut off and placed in 1:1 HBSS:PBS (PBS containing 100 U / mL penicillin and 100 μg / mL streptomycin). The mixture was rinsed three times with 1:1 HBSS:PBS. The connective tissue around the hair follicle was carefully removed under a dissecting microscope. The hair follicle was bluntly separated from the surrounding tissue in the direction of the hair follicle using microsurgical forceps. The distal end of the hair follicle was gently grasped and the hair was carefully pulled back from the subcutaneous tissue. The separated hair follicles were placed in different wells of a 24-well plate.

[0082] 2.2 Hair follicle culture: Incubate at 37℃ and 5% CO2 for 14 days, with 1 ml of culture medium per well.

[0083] 2.3 Sample addition: On the second day of culture, add the culture medium containing different concentrations of samples into the corresponding wells. Set up 8 replicates for each group, and place 2-3 isolated hair follicles in each well. Change the medium every 3 days.

[0084] 2.4 Image Analysis: The length of the whisker hair follicles was photographed every 7 days, and morphological observation and measurement of the whisker hair follicle length were performed. Data Analysis: Data are expressed as mean ± standard deviation. The relative growth rate of hair follicles is calculated using a formula. The test results are shown in the table below. Figure 1 As shown.

[0085] 2. Keratin gene expression level The expression levels of keratin genes in human hair papilla cells after treatment with samples containing 5% of the composition from Examples 1 and 5 were tested, and the results are shown in the table below.

[0086] 3. IL-1β and IL-8 content tests.

[0087] RAW264.7 cells in good exponential growth phase were seeded into 12-well plates and cultured for 24 hours.

[0088] The complete culture medium in the wells was aspirated, and after starvation treatment with PBS for 3 hours, the blank control group was given complete culture medium, the model group was given LPS and complete culture medium, and the sample group was given LPS, complete culture medium and diluted sample (5% composition content sample of Example 1, Example 4 and Comparative Example 2) and cultured for 48 hours.

[0089] Collect the cell supernatant culture medium, centrifuge to obtain the supernatant, and follow the instructions in the ELISA kit manual.

[0090] Statistical analysis: All experiments were repeated at least three times according to the parallel design principle, and the experimental data are expressed as mean standard deviation (MeanSD).

[0091] The test results are shown in the table below.

[0092] The above description is merely a preferred embodiment of the present invention. The scope of protection of the present invention is not limited to the above embodiments. All technical solutions falling within the scope of the present invention's concept are within the scope of protection of the present invention. It should be noted that for those skilled in the art, any improvements and modifications made without departing from the principle of the present invention should also be considered within the scope of protection of the present invention.

Claims

1. A hair care composition with oil-controlling, anti-hair loss, and repairing effects, characterized in that, The components include the following parts by weight: Myristoyl pentapeptide-4: 0.00001 to 0.0001 parts; Palmitoyl tripeptide-1: 0.00002 to 0.0002 parts; Acetyl tetrapeptide-3: 0.00001–0.00015 parts; Acetyl hexapeptide-8: 0.00002–0.00006 parts; Palmitoyl tetrapeptide-7: 0.000004–0.00002 parts; Hexapeptide-3: 0.00001 to 0.0001 parts; Water: 96-98 parts; Glycerin: 0.1 to 0.2 parts.

2. The hair care composition with oil-controlling, anti-hair loss, and repairing effects according to claim 1, characterized in that: The weight ratio of myristoyl pentapeptide-4, palmitoyl tripeptide-1, acetyl tetrapeptide-3, and hexapeptide-3 is 1:(0.8-1.2):(0.5-1.5):(0.8-1.2).

3. The hair care composition with oil-controlling, anti-hair loss, and repairing effects according to claim 1, characterized in that: It also includes 0.001 to 0.005 parts by weight of silver linden extract.

4. The hair care composition with oil-controlling, anti-hair loss, and repairing effects according to claim 1, characterized in that: The pH value of the composition is 5.2 to 6.

5.

5. The hair care composition with oil-controlling, anti-hair loss, and repairing effects according to claim 1, characterized in that: The myristoyl pentapeptide-4 and palmitoyl tripeptide-1 are modified with C14-C16 straight-chain fatty acyl groups, respectively, and the acetyl tetrapeptide-3 is modified with N-terminal acetylation.

6. The hair care composition with oil-controlling, anti-hair loss, and repairing effects according to claim 1, characterized in that: Also added is 0.002 to 0.004 parts by weight of a penetration enhancer, wherein the penetration enhancer is selected from one or more of propylene glycol, hexanediol, butanediol and pentanediol.

7. The hair care composition with oil-controlling, anti-hair loss, and repairing effects according to claim 1, characterized in that: The mass ratio of the acetyl tetrapeptide-3 to hexapeptide-3 combination is 1:(1.2 to 2.5).

8. A method for preparing a hair care composition with oil-controlling, anti-hair loss, and repairing effects according to any one of claims 1-7, characterized in that, Includes the following steps: S1: Weigh out myristoyl pentapeptide-4, palmitoyl tripeptide-1, acetyl tetrapeptide-3, hexapeptide-3, acetyl hexapeptide-8 and palmitoyl tetrapeptide-7, and mix them according to the weight proportions described in the formula; S2: Mix the peptide components from step S1 with glycerin in a predetermined ratio, stir until completely homogeneous, then heat to 40-50℃, maintain this temperature range and stir for 10-20 minutes until all components are completely dissolved and a transparent homogeneous solution is formed. S3: Add water to the above solution and continue stirring until a uniformly dispersed solution is obtained; S4: Adjust the pH of the solution to 5.2-6.5 to ensure that all components are under ideal self-assembly conditions; S5: The above solution is subjected to ultrasonic treatment; S6: Cool the mixture obtained in step S5 to 20-25°C and remove moisture under vacuum conditions to obtain the hair care composition with oil control, anti-hair loss and repair effects.

9. A method for preparing a hair care composition with oil-controlling, anti-hair loss, and repairing effects according to claim 8, characterized in that, Following step S3, the following optimization steps are also included: Optimization step 1: Let the homogeneous solution containing polypeptide, glycerol and water stand at a constant temperature of 25-30°C for 60-120 minutes; Optimization step 2: After the settling period is complete, pH adjustment is performed. The pH adjustment is carried out at a stirring speed not exceeding 100 rpm, and the adjustment speed is controlled at 0.05 to 0.1 pH units per minute.

10. A method for preparing a hair care composition with oil-controlling, anti-hair loss, and repairing effects according to claim 8, characterized in that, Following the S5 ultrasonic processing step, the following optimization steps are also included: Optimization step 3: The ultrasonically treated solution is allowed to stand at 20-25℃ for 30-60 minutes to form a stable self-assembled micelle structure with a particle size controlled between 30-100nm and a particle size distribution index not higher than 0.

25. Optimization step 4: Sterilization filtration is performed through a microporous membrane with a pore size of 0.22μm to remove large molecular aggregates that are not in a micelle state.

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