Composition for repairing and relieving light damage after medical art and application thereof

Abstract

The invention relates to a composition for repairing and relieving light damage after medical art and application of the composition, and belongs to the technical field of cosmetics. The composition is prepared from a camellia flower extract, fibroin, XVII type collagen, XXI type collagen and meadowfoam seed oil. The composition realizes multi-dimensional integrated management of physical barrier, microbial barrier, environmental stress defense and neuropsychological stress intervention through precise synergy of all the components on molecule, cell and system levels, relieves the risk of'aging 'of postoperative skin caused by blue light, sunlight and pressure in daily life, implants emotional skin care experience, and has a good application prospect. A'microorganism-skin barrier-neural immunity 'three-dimensional synergistic effect network is constructed, micro-ecological regulation and control, environmental stress defense and neuropsychological pacification are integrated, and normal form transformation from passive repair to active system stability maintenance after medical art is achieved.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of cosmetic technology, in particular to a composition for repairing and soothing post-medical aesthetic light damage and application thereof. BACKGROUND

[0002] With the increasing demand for post-medical aesthetic repair during the critical period, the skin not only faces the challenges of impaired barrier function and exacerbated inflammatory response after surgery, but also is affected by environmental and psychological stress factors in modern life, such as screen blue light, sun exposure, and staying up late. These factors can induce postoperative sensitivity and lead to signs of aging, including increased oxidative stress, microbial imbalance, increased release of inflammatory mediators, and mood-related skin problems (such as sensitivity, dryness, and signs of premature aging).

[0003] In the prior art, for the repair of post-aesthetic skin barrier damage, the conventional solution focuses on providing immediate repair of the physical barrier and basic anti-inflammatory. These solutions usually rely on high concentrations of hygroscopic ingredients such as sodium hyaluronate, and film-forming substances such as panthenol, to reduce trans-epidermal water loss (TEWL) by forming a protective film on the skin surface. At the same time, exogenous supplementation of collagen or its hydrolysates, and the application of biologically active ingredients such as epidermal growth factor (EGF) or copper peptides, aim to simulate the extracellular matrix and promote wound healing. In addition, the addition of non-steroidal anti-inflammatory ingredients such as glycyrrhizinic acid or asiaticoside is used to inhibit acute inflammatory reactions after surgery. However, these prior art solutions have obvious limitations: first, their mechanism of action is mostly passive and physical repair, lacking active regulation and homeostasis of the important biological barrier of the skin microbiome, which can lead to the proliferation of opportunistic pathogens and cause persistent micro-inflammatory states, thereby delaying the repair process; second, existing solutions generally ignore the superimposed negative effects of persistent stressors in modern living environments, such as high-energy visible light (HEV, commonly known as blue light), circadian rhythm disorders (caused by behaviors such as staying up late), and mental stress, which can induce a sharp increase in intracellular oxidative stress, activate inflammatory signaling pathways such as nuclear factor kappa B (NF-κB), and disrupt the expression of circadian rhythm genes inherent to the skin, collectively exacerbating the phenomenon of "secondary environmental aging", manifested as delayed repair, abnormal pigmentation, and accelerated collagen degradation; third, existing technologies completely separate the intrinsic link between the skin system and the central nervous system (i.e., the "brain-skin axis"), failing to intervene in the psychological stress caused by aesthetic surgery itself and postoperative anxiety, which can lead to an increase in stress hormones such as cortisol through the hypothalamic-pituitary-adrenal (HPA) axis, thereby amplifying local inflammatory reactions and disrupting the integrity of the skin barrier function. Therefore, there is an urgent need to provide an innovative skincare composition that builds a multi-target, active, and synergistic care system to achieve comprehensive repair and defense of post-aesthetic skin. SUMMARY

[0004] The purpose of the present application is to overcome the shortcomings of the prior art and provide a composition for post-aesthetic photodamage repair and relief and its application.

[0005] To achieve the above-mentioned purpose, the technical solution adopted by the present application is as follows: In a first aspect, the present application provides a composition comprising camellia flower extract, silk fibroin, collagen type XVII, collagen type XXI, and white pool flower seed oil in a weight ratio of (0.5-5):(0.01-1):(0.01-1):(0.01-1):(0.1-3).

[0006] The present application combines camellia extract, silk fibroin, collagen type XVII, collagen type XXI and white pool flower seed oil in a specific ratio, and through the synergistic effect of active components in the composition, achieves systematic repair and protection of post-medical beauty skin in multiple dimensions. Specifically, in the system regulation of microecology and barrier: through the synergistic effect of camellia extract, collagen type XVII, collagen type XXI, white pool flower seed oil and silk fibroin, a complete barrier repair system from microecological defense to deep structure support is constructed. Specifically, the polyphenolic components in camellia extract can selectively inhibit the excessive proliferation of pathogenic bacteria such as propionibacterium acnes, while promoting the colonization of beneficial symbiotic bacteria such as staphylococcus epidermidis, thereby actively maintaining the healthy homeostasis of microecology; white pool flower seed oil forms an efficient defense barrier inside the skin due to its excellent antioxidant capacity, can quickly neutralize the excess free radicals generated by blue light, stress and other environmental factors, prevent oxidative stress from causing damage to cells and microecological balance, and create a stable internal environment for subsequent repair; on this basis, collagen type XVII, as the core structural protein of hemidesmosome, is tightly combined with the epidermal basal layer cells and dermal anchoring fibrils through its transmembrane domain, directly strengthening the mechanical stability of the dermal-epidermal junction (DEJ), providing a key anchoring point for the stable attachment of epidermal cells, and effectively preventing epidermal exfoliation caused by physical friction or aging; and collagen type XXI, as a key signaling molecule, promotes the neogenesis and ordered assembly of collagen types I, III, IV, VII and XVII, driving structural repair from the deep, that is, collagen type XVII ensures the stability of the cell “root” and activates regeneration, and collagen type XXI provides comprehensive “repair materials”, which together enhance the physical anchoring and biological defense capacity of the skin; in addition, silk fibroin and white pool flower seed oil synergistically act on the skin surface: silk fibroin forms an efficient moisturizing barrier through its excellent film-forming property, significantly reducing transdermal water loss; white pool flower seed oil further reinforces this barrier with its rich lipid components, together creating a suitable and safe physical environment for the stable proliferation of microecology. Ultimately, these components work together through multi-target collaboration to build an integrated barrier system that dynamically responds and layer-by-layer reinforces from microecology and oxidative defense (camellia extract, white pool flower seed oil), to cell regeneration root (collagen type XVII), to structural remodeling (collagen type XXI), and to surface water locking and barrier consolidation (silk fibroin, white pool flower seed oil). In terms of environmental stress defense: camellia extract effectively quenches reactive oxygen species generated by blue light and inflammation, inhibits the activation of NF-κB pathway and the overexpression of matrix metalloproteinase, and protects the dermal matrix; in combination with the synergistic resistance of silk fibroin and collagen to oxidative stress and the regulation function of circadian rhythm genes, it jointly resists secondary environmental aging. In the aspect of neuro-psychological intervention: white pool flower seed oil indirectly regulates the “brain-skin axis” due to its excellent antioxidant and barrier repair functions.It can efficiently quench free radicals, reduce skin oxidative stress, and thus transmit a calming signal to the central nervous system. At the same time, its repaired physical barrier can effectively reduce the activation of nerve endings by external stimuli, thereby inhibiting the excessive release of neuropeptides such as substance P from the source. Ultimately, this mechanism breaks the vicious cycle of "stress-barrier damage-neuroinflammation", and synergistically achieves the co-homeostasis of mood and skin.

[0007] After effect testing, the composition provided by the present application has a synergistic effect in promoting collagen regeneration and skin barrier repair, improving environmental stress resistance, anti-inflammatory ability, and maintaining facial microbial flora homeostasis. The composition can significantly repair the skin barrier of a subject after medical aesthetics and improve the subject's defense ability against environmental stress (oxidative stress) and blue light damage, achieving a synergistic care that integrates postoperative soothing repair, anti-photoaging, emotional soothing, and anti-inflammatory stability. In summary, the composition provided by the present application is not a simple superposition of existing components, but a precise synergy of each component at the molecular, cellular, and systemic levels, achieving multi-dimensional integrated management of physical barrier, microbial barrier, environmental stress defense, and neuro-psychological stress intervention.

[0008] Further, the composition comprises Camellia japonica extract, silk fibroin, collagen type XVII, collagen type XXI, and white pool flower seed oil in a weight ratio of (1-4):(0.1-0.8):(0.1-0.5):(0.1-0.5):(0.5-2).

[0009] Preferably, the composition comprises Camellia japonica extract, silk fibroin, collagen type XVII, collagen type XXI, and white pool flower seed oil in a weight ratio of (1.5-3):(0.2-0.5):(0.2-0.3):(0.2-0.3):(0.8-1.5).

[0010] In a second aspect, the present application provides the use of the composition of the first aspect in the preparation of a cosmetic product having the effects of post-medical-aesthetic light damage repair and soothing.

[0011] Further, the cosmetic product is a cosmetic water, cream, mask, serum, or freeze-dried powder.

[0012] Preferably, the cosmetic product is a serum.

[0013] Further, the added amount of the composition is 0.1%-10% of the total weight of the cosmetic product.

[0014] Preferably, the added amount of the composition is 0.5%-3% of the total weight of the cosmetic product.

[0015] In a third aspect, the present application provides an essence, comprising the following raw materials by weight percentage: 0.1%-10% of the composition of the first aspect, 0.01%-1% of a thickening agent, 0.5%-5% of a humectant, 0.01%-0.5% of a pH regulator, 0.1%-2% of a preservative, and the rest being deionized water.

[0016] Further, the essence comprises the following components by weight percentage: 0.5%-3% of the composition of the first aspect, 0.05%-0.8% of a thickening agent, 0.5%-3% of a humectant, 0.01%-0.3% of a pH regulator, 0.2%-2% of a preservative, and the rest being deionized water.

[0017] Preferably, the essence comprises at least one of the following (a)-(d): (a) the thickening agent comprises at least one of xanthan gum, carbomer, hydroxyethyl acrylate / acryloyl dimethyl taurate sodium copolymer, acryloyl dimethyl ammonium taurate / VP copolymer, and sclerotium gum; (b) the humectant comprises at least one of allantoin, betaine, beta-glucan, trehalose, glycol, dipropylene glycol, sodium hyaluronate, 1,3-butanediol, glycerol, D-panthenol, and ceramide; (c) the pH regulator comprises at least one of arginine, tromethamine, and triethanolamine; (d) the preservative comprises at least one of 1,3-propanediol, 1,2-hexanediol, and p-hydroxyacetophenone.

[0018] Further, the preparation method of the essence comprises the following steps: S1, mixing the humectant, the thickening agent, and 1 / 2 of the deionized water, heating to 80°C and homogenizing uniformly to obtain a premix; S2, heating the preservative to melt, when the temperature of the premix of S1 drops to 60°C, adding the preservative and stirring uniformly, when the temperature of the mixture drops to 40°C, adding the composition and the remaining deionized water, stirring uniformly, finally adding the pH regulator to adjust the pH to obtain the essence.

[0019] Compared with the prior art, the present application has the following beneficial effects: The present application provides a composition for repairing and soothing post-medical art light damage, which comprises camellia flower extract, fibroin, collagen type XVII, collagen type XXI and white pool flower seed oil. The composition can effectively promote collagen regeneration and skin barrier repair, improve skin resistance to environmental stress and anti-inflammatory ability, maintain facial microbial flora homeostasis, regulate microbial flora for postoperative soothing repair, reduce the risk of "rejuvenation" of postoperative skin caused by blue light and sunlight environment in daily life, and implant the experience of emotional skin care, build a "microbial-skin barrier-neuroimmune" three-dimensional synergistic network, integrate microecological regulation, environmental stress defense and neuro-psychological pacification into one, and realize the paradigm shift from passive repair to active system stability maintenance after medical art. DETAILED DESCRIPTION

[0020] In order to better illustrate the purpose, technical scheme and advantages of the present application, the present application will be further described below in combination with specific examples.

[0021] The camellia flower extract is from Clariant - Personal Care Company, and the trade name is RedSnow. ® ; The fibroin is from Myogenica (Guangzhou) Biotechnology Co., Ltd., and the trade name is soluble fibroin (LMW), batch number FBIL250227CQ. The collagen type XVII is from Shanxi Jinbo Biomedicine Co., Ltd., and the trade name is ARHC XVII powder. The collagen type XXI is from Mind Cure Company, and the trade name is Yuyan ® CureColla ® 21; The white pool flower seed oil is from Hangzhou Jiajiale Biotechnology Co., Ltd., and the trade name is JAJALE ® Moisture white pool flower seed oil.

[0022] Other materials, reagents, etc. used in the examples can be obtained from commercial channels if not specifically stated.

[0023] Examples 1-7 and Comparative Examples 1-6 The components in the compositions of each example and each comparative example were mixed according to the formula amount to obtain the compositions of examples 1-7 and comparative examples 1-6.

[0024] Table 1 Components of the composition and their weight ratio

[0025] Note: The total weight of the compositions in different examples in Table 1 is the same.

[0026] Comparative Example 1 Comparative Example 1 does not contain camellia extract compared with Example 4, and the missing amount is supplemented by silk fibroin, collagen type XVII, collagen type XXI, and white pool flower seed oil at a weight ratio of 0.45:0.25:0.25:1.

[0027] Comparative Example 2 Comparative Example 2 does not contain silk fibroin compared with Example 4, and the missing amount is supplemented by camellia extract, collagen type XVII, collagen type XXI, and white pool flower seed oil at a weight ratio of 2:0.25:0.25:1.

[0028] Comparative Example 3 Comparative Example 3 does not contain collagen type XVII compared with Example 4, and the missing amount is supplemented by camellia extract, silk fibroin, collagen type XXI, and white pool flower seed oil at a weight ratio of 2:0.45:0.25:1.

[0029] Comparative Example 4 Comparative Example 4 does not contain collagen type XXI compared with Example 4, and the missing amount is supplemented by camellia extract, silk fibroin, collagen type XVII, and white pool flower seed oil at a weight ratio of 2:0.45:0.25:1.

[0030] Comparative Example 5 Comparative Example 5 does not contain white pool flower seed oil compared with Example 4, and the missing amount is supplemented by camellia extract, silk fibroin, collagen type XVII, and collagen type XXI at a weight ratio of 2:0.45:0.25:0.25.

[0031] Comparative Example 6 Comparative Example 6 has a weight ratio of camellia extract, silk fibroin, collagen type XVII, collagen type XXI, and white pool flower seed oil of 0.3:0.05:2:0.05:4.5 compared with Example 4.

[0032] Test Example 1 Test of the Effect of the Composition on Promoting Collagen Regeneration and Barrier Repair The present test example aims to verify the synergistic effect of the composition of the present application in promoting skin barrier repair, accelerating wound epithelialization, and enhancing cell junction from the in vitro cell level.

[0033] Test principle: In the postoperative repair process of medical aesthetics, the damage of skin barrier and the integrity of dermal-epidermal junction structure is a key problem, among which type VII collagen as the main component of anchoring fiber plays a core role in maintaining the stability of basement membrane zone and the adhesion between epidermis and dermis, and its expression level directly reflects the repair ability of skin tissue structure; at the same time, the cell scratch experiment can directly reflect the healing dynamics of cell layer after physical damage by simulating the migration and proliferation behavior of epidermal cells after damage, so as to evaluate the re-epithelialization efficiency at the cellular level in the repair process. Therefore, the test example detects the content of type VII collagen of cells after treatment by ELISA and evaluates the repair ability of the composition by cell scratch experiment.

[0034] 1. Promote collagen regeneration: Experimental model: human keratinocytes (HaCaT) were used.

[0035] Experimental model culture: the cell line used was human keratinocytes HaCaT (Shanghai Yaji Biological Technology Co., Ltd.), and the culture conditions were as follows: incubator temperature 37±1°C, humidity 90±5%, carbon dioxide 5±1%; the cells were cultured and treated according to the grouping, and then the test was carried out, and the test method was as follows: (a) Preparation of sample solution: the compositions of Examples 1-7 and Comparative Examples 1-6 were dissolved with quantitative DMSO and then diluted with DMEM medium to obtain a sample solution with a final concentration of 0.1% (v / v).

[0036] (b) Resuscitation of HaCaT cells: the cell suspension was inoculated in a 96-well cell culture plate at a density of 2000 cells / well, 100 μL of DMEM culture medium containing 10% (v / v) fetal bovine serum (FBS) + 1% (v / v) penicillin / streptomycin was added to each well, and the cells were cultured for 24 h.

[0037] (c) Sampling: the supernatant was discarded, 100 μL of DMEM medium was added to the blank group, and 100 μL of sample solution containing 0.1% of the corresponding composition was added to the sample group, and the cells were cultured for 12 h and 24 h. The culture medium was discarded and the cells were washed with PBS for 2 times. 1 mL of TRIzol lysis solution was added, the cells were scraped, transferred to an EP tube, and stored at -80°C for standby.

[0038] (d) ELISA detection of protein content: the content of type VII collagen protein in HaCaT cells was detected using human type VII collagen (COL7) ELISA detection kit (Shanghai Keaibo Biological, item number CB18922-Hu). The type VII collagen expression promotion rate was calculated according to the following formula, each test was repeated 3 times, and the average value was taken as the result, which was shown in Table 2.

[0039] Protein expression promotion rate = [ | (protein expression amount sample group - protein expression amount blank group) / protein expression amount blank group] x 100% 2. Cell scratch test: Experimental model: human keratinocytes (HaCaT) Experimental model culture: the cell line used was human keratinocytes HaCaT (Shanghai Yaji Biotechnology Co., Ltd.), and the culture conditions were as follows: incubator temperature 37 ± 1°C, humidity 90 ± 5%, carbon dioxide 5 ± 1%; the cells were cultured and treated according to the grouping, and then tested, and the test method was as follows: (a) Sample solution preparation: each component in the compositions of Examples 1-7 and Comparative Examples 1-6 was dissolved with quantitative DMSO and then diluted with DMEM medium, and the final concentration of the experiment was 0.1% (v / v).

[0040] (b) Resuscitation of HaCaT cells: the cell suspension was inoculated in a 6-well cell culture plate, with a density of 10 x 10 5 cells / well, and 2 mL of DMEM culture medium containing 10% (v / v) fetal bovine serum (FBS) + 1% (v / v) penicillin / streptomycin was added to each well, and the cells were cultured for 24 h.

[0041] (c) Scratch: after 24 h, the cells in the 6-well plate were observed to be fully covered with a single layer, and a gun head was used to draw a straight line perpendicular to the back horizontal line, and 2 lines were drawn in each well.

[0042] (d) Sample: the cells were washed with PBS 3 times to remove the suspended cells scratched off. According to the grouping, 1.8 mL of serum-free culture medium was added to the wells, and then 200 μL of the sample solution of 0.1% corresponding composition was added, and the same amount of DMEM culture medium was added to the cell control wells. Continue to place in a 37°C 5% CO2 incubator for culture. Take pictures regularly (0 h, 12 h and 24 h), and record the picture-taking position in each well. Fixed positions were observed and photographed during subsequent observation; each test was repeated 3 times.

[0043] The scratch area of the cell control wells and each sample well at 0 h, 12 h and 24 h was calculated by Image J software, each test was repeated 3 times, and the average value was taken; the cell healing rate was calculated, and the formula was as follows: Cell healing rate = [(A0-A t ) / A0] x 100%; Wherein, A0 represents the average value of the scratch area at 0 h, and A t represents the average value of the scratch area at t h.

[0044] The higher the promotion rate of the protein and the healing rate of the cell scratch, the stronger the ability of the composition to repair the barrier after operation, and the results are shown in Table 2.

[0045] From the results of Table 2, Examples 1-7, it can be seen that the composition provided by the present application can significantly increase the expression of collagen VII and the cell healing rate of human keratinocytes, and has the characteristics of short time and high efficiency; among them, the silk fibroin and camellia extract in the composition jointly promote the migration and proliferation of keratinocytes, accelerate the process of wound closure and epithelialization; the white pool flower seed oil simulates the skin lipid barrier to provide necessary closed protection and moisturizing microenvironment at the cell level, ensuring the normal physiological activities of cells. Collagen XVII as a key component of hemidesmosome directly mediates cell-matrix adhesion, and collagen XXI regulates the extracellular matrix homeostasis, both of which synergistically activate the function of keratinocytes and jointly promote the synthesis of collagen VII and cell migration process. Therefore, the interaction of each component in the composition promotes collagen regeneration and barrier repair.

[0046] From the comparison of the results of Example 4 and Comparative Examples 1-5 in Table 2, it can be seen that when any one of camellia extract, silk fibroin, collagen XVII, collagen XXI, and white pool flower seed oil is absent in the composition of the present application, the promoting effect of the composition on the expression of collagen XVII and the cell healing rate decreases, verifying the synergistic effect of camellia extract, silk fibroin, collagen XVII, collagen XXI, and white pool flower seed oil in the composition of the present application in improving the ability of the composition to promote collagen regeneration and barrier repair; from the comparison of the results of Example 4 and Comparative Example 6 in Table 2, it can be seen that the amount and ratio of each component in the composition will affect the efficacy of the composition in promoting collagen regeneration and barrier repair, and when the amount and ratio of each component are within the range defined in the present application, the composition has better effect.

[0047] Table 2 Collagen VII promotion rate and cell healing rate results

[0048] Test Example 2 Evaluation of the environmental stress resistance and anti-inflammatory ability of the composition This test example simulates the damage of screen blue light to the deep layer of skin, aiming to verify whether the composition can play a biological antioxidant role in living cells, protect mitochondrial function, and inhibit the generation of key enzyme MMP-1 of photoaging from the root, thereby protecting dermal collagen from degradation, which is an effective intervention for photoaging; and verify the ability of the composition to inhibit the inflammatory signaling pathway by simulating experiments, and verify whether it can alleviate neurogenic inflammation and microinflammatory state by reducing the release of core pro-inflammatory factors such as IL-1β and TNF-α, which is crucial for the relief, stability maintenance and blocking of the vicious cycle of “inflammation-aging” after medical aesthetics. The specific test content is as follows: 1. Environmental stress resistance test: Experimental model: normal human dermal fibroblasts (HDF).

[0049] Experimental model culture: The cell line used was human dermal fibroblasts (HDF) (Shanghai Yage Biotechnology Co., Ltd.), and the culture conditions were as follows: incubator temperature 37±1°C, humidity 90±5%, carbon dioxide 5±1%; the cells were cultured and treated according to the grouping, and then tested, and the testing method was as follows: (a) Sample solution preparation: The compositions of Examples 1-7 and Comparative Examples 1-6 were dissolved in a quantitative amount of DMSO and then diluted with DMEM medium, and the final concentration was 0.1% (v / v).

[0050] (b) Resuscitation of HDF cells: The cell suspension was inoculated in a 96-well cell culture plate at a density of 2000 cells / well, and 100 μL of DMEM culture medium containing 10% (v / v) fetal bovine serum (FBS) + 1% (v / v) penicillin / streptomycin was added to each well, and the cells were cultured for 24 h.

[0051] (c) Model establishment: The cells were divided into a model blank group and a sample group. Each group of cells was irradiated under a specific blue light source (wavelength: 415 nm, intensity: 30 J / cm²) to establish a damage model.

[0052] (d) Sample application: The supernatant was discarded, 100 μL of DMEM medium was added to the model blank group, and 100 μL of medium containing 0.1% of the corresponding composition of the sample solution was added to the sample group. The cells were cultured for 2 h, and then the medium was collected and washed twice with PBS. 1 mL of TRIzol lysis solution was added, the cells were scraped, transferred to an EP tube, and stored at -80°C for use. Each group of tests was repeated 3 times.

[0053] (e) ELISA detection of protein content: The expression of MMP-1 in HDFs cells was detected using a human matrix metalloproteinase 1 (MMP-1) ELISA kit (Shanghai Enzyme Link, item number ml038199).

[0054] MMP-1 inhibition rate = [1 - (MMP-1 expression 样品组 / MMP-1 expression 模型空白组 )] × 100%.

[0055] 2. Anti-inflammatory ability test: Experimental model: RAW264.7 mouse macrophages.

[0056] Experimental model culture: RAW264.7 mouse macrophages were inoculated in a T75 culture flask, and the culture conditions were as follows: incubator temperature 37±1°C, humidity 90±5%, carbon dioxide 5±1%; the cells were cultured and treated according to the grouping, and then tested, and the testing method was as follows: (a) Sample liquid preparation: each example and comparative composition was dissolved with quantitative DMSO and then diluted with DMEM medium, and the final concentration was 0.1% (v / v).

[0057] (b) Resuscitation of RAW264.7 cells: the cell suspension was inoculated in a 96-well cell culture plate at a density of 2000 cells per well, and 100 μL of DMEM medium containing 10% (v / v) fetal bovine serum (FBS) + 1% (v / v) penicillin / streptomycin was added to each well, and the cells were cultured for 24 h.

[0058] (c) Model establishment: the cells were divided into a model blank group and a sample group. Each group was pretreated with a LPS-containing medium for 12 h to establish an inflammation model.

[0059] (d) Sample addition: the supernatant of the cells was discarded, and 100 μL of DMEM medium was added to the model blank group. 100 μL of medium containing 0.1% of the corresponding sample liquid was added to the sample group, and the cells were cultured for 2 h. The cells in each group were collected, and the medium was discarded. The cells were washed twice with PBS. 1 mL of TRIzol lysis solution was added, the cells were scraped, and the cells were transferred to an EP tube and stored at -80°C for standby. Each test was repeated 3 times.

[0060] (e) ELISA detection of protein content: mouse tumor necrosis factor alpha (TNF-α) kit (Shanghai Enzyme Link, item number ml002095) and mouse interleukin 1 beta (IL-1β) ELISA kit (Shanghai Enzyme Link, item number ml106733) were used to detect the levels of inflammatory factors such as TNF-α and IL-1β in RAW264.7 cells.

[0061] Calculation of inhibition rate: inhibition rate = [1 - (X 样品组 / X 模型空白组 )] × 100%; The greater the inhibition rate of the expression of the protease and the inhibition rate of the inflammatory factor, the stronger the anti-environmental stress and anti-inflammatory ability of the composition after the operation. The calculated results are shown in Table 3.

[0062] According to the results in Table 3, the composition provided by the application has a significant effect of inhibiting the expression of MMP-1 enzyme, TNF-α and IL-1β verification factor, which indicates that the composition has high anti-environmental stress and anti-inflammatory ability, and can effectively intervene and relieve photaging and neurogenic inflammation and microinflammation. From the results of Example 4 and Comparative Examples 1-6 in Table 3, it can be seen that the specific component composition and the component ratio within a certain range of the composition have a significant influence on the anti-environmental stress and anti-inflammatory ability of the composition.

[0063] Table 3 Determination results of anti-environmental stress and anti-inflammatory ability of the composition Group MMP-1 inhibition rate (%) TNF-α inhibition rate (%) IL-1β inhibition rate (%) Example 1 40.8 40.8 37.7 Example 2 47.4 47.5 42.3 Example 3 51.2 50.2 46.6 Example 4 57.7 53.1 49.5 Example 5 55.3 48.9 47.8 Example 6 44.5 45.6 39.4 Example 7 43.6 42.4 38.2 Comparative Example 1 15.2 16.7 11.1 Comparative Example 2 18.3 19.5 14.9 Comparative Example 3 16.9 18.8 15.4 Comparative Example 4 16.5 20.6 16.7 Comparative Example 5 14.1 14.8 13.1 Comparative Example 6 22.8 25.3 17.6 Test Example 3: Determination of the effect of the composition on the inhibition of inflammation induced by P. acnes in zebrafish Medical aesthetic operations can destroy the facial microorganism homeostasis, and can cause the excessive proliferation of pathogenic bacteria such as Staphylococcus aureus and Propionibacterium acnes, and further cause inflammation and acne. In order to evaluate the potential of the composition to relieve inflammation caused by the imbalance of the microorganism flora, the test example constructs a zebrafish inflammation model induced by Staphylococcus aureus and Propionibacterium acnes, and evaluates the ability of the composition to effectively inhibit inflammation caused by the imbalance of the flora after medical aesthetic operation by observing and analyzing the immune migration and aggregation behavior of neutrophils to the skin epidermis. The experimental method is as follows: (1) Animal model: zebrafish embryos 6-8 hours after fertilization (screening criteria: normal morphology, developmental stage is blastula stage).

[0064] (2) Preparation of culture solution and bacterial solution: the culture solution uses zebrafish special embryo culture solution (containing methyl blue antifungal), and the mixed bacterial solution of Staphylococcus aureus and Propionibacterium acnes (2x10 7 CFU / mL, mixed at a ratio of 1:1) is used to model.

[0065] According to the grouping, the embryo culture and treatment are carried out, and then the test is carried out, and the treatment method is as follows: a. Sample preparation: dissolve the compositions of Examples 1-7 and Comparative Examples 1-6 in the fish embryo culture solution to obtain a fish embryo culture solution with a mass concentration of 10wt%.

[0066] b. The experiment is divided into three groups: Blank group: 200 μL of embryo culture solution + normally developed zebrafish; Control group: 200 μL of embryo culture solution + zebrafish injected with 10 μL of mixed bacterial solution in the tail fin; Sample group: 200 μL of embryo culture solution containing 10wt% of the composition of Examples 1-7 or Comparative Examples 1-6 + zebrafish injected with 10 μL of mixed bacterial solution in the tail fin.

[0067] c. Sample: collect healthy zebrafish embryos 6-8 hours after fertilization, wash and transfer to a 24-well plate. According to the experimental grouping, 200 μL of the corresponding culture solution (blank group, control group and sample group) is added to each well, and the bacterial solution is injected into the tail fin according to the grouping, at least 10 embryos per group, and placed in a constant temperature incubator at a temperature of 28°C for 3 hours.

[0068] (3) Data analysis: 10 zebrafish were randomly selected from each test group and placed under a fluorescence microscope for photography. Image J, a high-level image processing software, was used to analyze and collect data. The rectangular tool was used to accurately demarcate the target area, and the average fluorescence intensity of the selected area was measured using the gray intensity method, i.e., the number of zebrafish neutrophils.

[0069] The inhibition rate of zebrafish neutrophils was calculated according to the calculation formula, and the higher the inhibition rate, the better the effect of the composition on inhibiting inflammation caused by imbalance of flora after medical art. The calculation formula is as follows: ; The results are shown in Table 4. The composition provided by the embodiment of the application has a neutrophil inhibition rate of 40.1%-55.8% on zebrafish, which is 13.7%-40.1% higher than the neutrophil inhibition rate of the compositions of comparative examples 1-6. It shows that the component composition and component ratio of the composition have a significant influence on the neutrophil inhibition effect of the composition. Under the combined influence of specific components and ratios, the composition provided by the embodiment of the application can synergistically improve the inhibition effect of neutrophils, thereby reflecting the high inhibition effect of the composition on inflammation caused by imbalance of flora after medical art.

[0070] Table 4 Results of neutrophil inhibition rate of the composition on zebrafish Group Neutrophil inhibition rate (%) Example 1 40.1 Example 2 48.7 Example 3 50.9 Example 4 55.8 Example 5 52.6 Example 6 45.4 Example 7 44.3 Comparative Example 1 15.7 Comparative Example 2 20.6 Comparative Example 3 21.4 Comparative Example 4 24.2 Comparative Example 5 17.5 Comparative Example 6 26.4 Application examples 1-7 and comparative application examples 1-6 The compositions of the examples and the comparative examples were added to the serum at a concentration of 3wt%, respectively, to obtain the serum of application examples 1-7 and comparative application examples 1-6, respectively. In addition, a serum without adding the composition was prepared, which was a blank application example. The formula of the serum is shown in Table 5.

[0071] Table 5 Serum components and amounts

[0072] The preparation method of the serum is as follows: S1, mix the humectant, thickening agent and 1 / 2 amount of deionized water, heat to 80℃ and homogenize uniformly to obtain a premix; S2, melt the preservative, when the temperature of the premix of S1 drops to 60℃, add the preservative and stir uniformly, when the temperature of the mixture drops to 40℃, add the composition and the remaining deionized water, stir uniformly, finally add the pH adjuster to adjust the pH, to obtain the serum.

[0073] Effect test: 1. Human skin patch test To strictly verify the high safety and mildness of the product on sensitive skin, a strengthened human closed patch test was designed and implemented. 150 volunteers aged 20-50 years old were recruited, including 50% of self-reported sensitive skin, 10 volunteers in each group, and all the subjects signed the informed consent form. To simulate the common barrier damage state of sensitive skin, we used medical 3M tape to tear the test area on the flexor of the subject's arm for 5 times in succession to build a more rigorous test environment. Each group of 10 volunteers, then, an equal amount (0.020 mL - 0.025 mL) of test samples (application examples 1-7 or comparative application examples 1-6 of the serum), blank application examples of serum and pure water (negative control) were placed in 10-hole patch devices, and were applied to the pre-treated skin with low sensitization tape for 24 h. At 0, 24 and 48 h after removing the patch device, the skin reactions were observed and recorded by professional evaluators according to the standard (no reaction 0 points, weak erythema 1 point, moderate erythema 2 points, severe erythema with edema 3 points, extremely severe reaction 4 points). The final safety judgment standard is: if the average irritation score of the application example group is not higher than that of the blank control group, and no adverse reactions of grade 2 and above occur, it is proved that the product is safe and non-irritating, and is suitable for sensitive skin. The adverse reactions of the skin are shown in Table 6 below.

[0074] Table 6 Skin reaction grading standard Score level Skin reaction 0 Negative reaction 1 Suspected reaction, only weak erythema 2 Weak positive reaction (erythema reaction): erythema, infiltration, edema, and possibly papules 3 Strong positive reaction (vesicle reaction): erythema, infiltration, edema, papules; reaction can exceed the test area 4 Very strong positive reaction (fusion vesicle reaction): obvious erythema, severe infiltration, edema, and fusion vesicles; reaction exceeds the test area After testing, the average irritation scores of the serum provided by application examples 1-7, comparative application examples 1-6 and blank application examples of the present application were not higher than that of the blank control group, and no adverse reactions of grade 2 and above occurred, indicating that the serum provided by the present application is suitable for sensitive skin, and is safe and non-irritating to human skin.

[0075] 2. Efficacy determination of serum on human body half a month after receiving medical beauty project Experimental method: According to the human efficacy evaluation principle of "Cosmetic Safety Technology Specification" (2015 edition), this experiment aims to scientifically evaluate the safety and effectiveness of the serum on the subjects half a month after receiving light to moderate medical beauty projects (such as non-exfoliating fractional laser, IPL) in the following aspects: (1) Core repair efficacy: skin barrier function (trans epidermal water loss amount TEWL) and erythema condition (a value).

[0076] (2) Anti-photoaging efficacy: improving the ability to defend against blue light and fine lines.

[0077] A total of 42 volunteers were recruited, all of whom met the following criteria: aged 18-35 years, in good health. It was planned that they would undergo a non-exfoliating / micro-exfoliating medical aesthetic procedure in the near future and have a good post-procedure recovery. They had mild to moderate fine lines in the target area of the face. They used electronic devices (mobile phones, computers) for more than 4 hours a day to ensure a background of blue light exposure. They voluntarily signed an informed consent form. All the subjects were randomly divided into 14 groups, with 3 people in each group. The volunteers enrolled were scheduled to use the obtained serum once a day, in the morning and evening, for a period of 4 weeks, and were followed up on day 1 (initial value) and day 28, and the TEWL value of the skin was detected by the Tewameter TM Hex trans-epidermal water loss probe, the erythema index (a* value) of the skin was detected by the MX 18 probe, and the wrinkles of the volunteers' skin were photographed and analyzed by VISIA-CR to evaluate the improvement of fine lines; the effect of the product on preventing blue light-induced pigmentation was evaluated by the number of brown spots analyzed by VISIA®. All tests were performed after the face was cleaned with a unified cleansing and the subjects sat in a controlled environment (temperature 20 ± 1°C, humidity 50 ± 10%) for 30 minutes. The barrier repair ability of the composition was represented by the improvement of the TEWL value, the soothing ability was represented by the degree of redness, the ability to resist environmental stress was represented by the improvement of blue light-induced pigmentation of brown spots, and the ability to resist photoaging was represented by the improvement of fine lines. The efficacy of the serum was represented by the improvement rate, and the calculation formula was as follows: Improvement rate (%) = [|(X mean value after use - X mean value before use)| / X mean value before use] x 100%; The results obtained are shown in Table 7. Blue light, sun exposure, lack of sleep and stress in daily life can cause post-procedure sensitivity and lead to signs of aging, such as increased fine lines and pigmentation. The use of the serum of application examples 1-7 of the present application can significantly improve the TEWL value of the skin, reduce the appearance of redness, fine lines and brown spots, indicating that the serum of application examples 1-7 of the present application can effectively repair the skin barrier damage that occurs after medical aesthetic procedures, soothe the skin and improve the volunteers' ability to resist photoaging. From the comparison of the data of comparative application examples 1-6 and application example 4, it can be seen that the camellia extract, silk fibroin, collagen type XVII, collagen type XXI and white pool flower seed oil in the composition provided by the present application can exert a synergistic effect within a specific ratio range, and together improve the skin repair ability and the ability to resist photoaging of the composition during the post-procedure repair period, thereby avoiding the signs of aging after medical aesthetic procedures.

[0078] Table 7 Results of human efficacy determination Group Improvement rate of skin TEWL (%) Improvement rate of skin erythema (%) Improvement rate of fine lines (%) Improvement rate of brown spots (%) Application Example 1 23.7 17.6 20.8 20.7 Application Example 2 29.1 19.8 22.7 22.4 Application Example 3 31.6 21.3 23.9 24.9 Application Example 4 33.2 29.7 26.9 27.2 Application Example 5 30.8 24.2 24.4 23.8 Application Example 6 27.6 18.9 23.5 23.5 Application Example 7 25.4 17.1 21.3 21.3 Comparative Application Example 1 10.2 6.9 10.1 6.2 Comparative Application Example 2 11.4 8.5 8.8 9.6 Comparative Application Example 3 13.5 8.6 7.2 7.8 Comparative Application Example 4 13.7 7.3 8.4 8.6 Comparative Application Example 5 9.1 6.7 9.4 5.1 Comparative Application Example 6 15.8 9.5 12.9 10.3 Blank Application Example 3.1 1.2 0.4 0.8 Finally, it should be noted that the above examples are merely intended to illustrate the technical solutions of the present application and not to limit the protection scope of the present application. Although the present application has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present application can be modified or equivalently replaced without departing from the essence and scope of the technical solutions of the present application.

Claims

1. A composition characterized in that, Camellia japonica extract, silk fibroin, collagen type XVII, collagen type XXI, and white pool flower seed oil in a weight ratio of (0.5-5):(0.01-1):(0.01-1):(0.01-1):(0.1-3).

2. The composition of claim 1, wherein Camellia japonica extract, silk fibroin, collagen type XVII, collagen type XXI, and white pool flower seed oil in a weight ratio of (1-4):(0.1-0.8):(0.1-0.5):(0.1-0.5):(0.5-2).

3. The composition of claim 1, wherein Camellia japonica extract, silk fibroin, collagen type XVII, collagen type XXI, and white pool flower seed oil in a weight ratio of (1.5-3):(0.2-0.5):(0.2-0.3):(0.2-0.3):(0.8-1.5).

4. Use of the composition of any one of claims 1-3 in the preparation of a cosmetic product having the effects of post-medical aesthetic light damage repair and soothing.

5. The use according to claim 4, wherein the compound is ###0002### The cosmetic product is a cosmetic water, cream, mask, serum, or lyophilized powder.

6. Use according to claim 5, wherein The cosmetic product is a serum.

7. The use according to claim 4, wherein the compound is ###0002### The composition is added in an amount of 0.1%-10% of the total weight of the cosmetic product.

8. A serum, characterized by, The composition comprises the following raw materials in the following weight percentages: 0.1%-10% of the composition of any one of claims 1-3, 0.01%-1% of a thickening agent, 0.5%-5% of a humectant, 0.01%-0.5% of a pH adjuster, 0.1%-2% of a preservative, and the balance being deionized water.

9. The essence as described in claim 8, characterized in that, The composition comprises the following raw materials in the following weight percentages: 0.5%-3% of the composition of any one of claims 1-3, 0.05%-0.8% of a thickening agent, 0.5%-3% of a humectant, 0.01%-0.3% of a pH adjuster, 0.2%-2% of a preservative, and the balance being deionized water.

10. The essence as described in claim 8, characterized in that, The composition comprises at least one of (a)-(d) below: (a) the thickening agent comprises at least one of xanthan gum, carbomer, hydroxyethyl acrylate / acryloyldimethyltaurate sodium copolymer, acryloyldimethyltaurate ammonium / VP copolymer, and sclerotium gum; (b) the humectant comprises at least one of allantoin, betaine, beta-glucan, trehalose, caprylyl glycol, dipropylene glycol, sodium hyaluronate, 1,3-butanediol, glycerin, D-panthenol, and ceramide; (c) the pH adjuster comprises at least one of arginine, tromethamine, and triethanolamine; (d) the preservative comprises at least one of 1,3-propanediol, 1,2-hexanediol, and other polyols, and p-hydroxyacetophenone.

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