Composition and application thereof in preparation of product for relieving constipation by promoting peristalsis

Abstract

The invention relates to the technical field of biological medicines, in particular to a composition and application thereof in preparation of a product for relieving constipation by promoting peristalsis. The composition comprises a honeysuckle flower extract and a hawthorn fruit extract, wherein the mass ratio of the honeysuckle extract to the hawthorn extract is 1: (1-80). Researches show that the composition obtained by combining the honeysuckle flower extract and the hawthorn fruit extract prepared by the method disclosed by the invention has the effect of promoting motilin secretion remarkably higher than that of the single honeysuckle flower extract and hawthorn fruit extract, and plays a synergistic effect. The composition disclosed by the invention has the effect of promoting secretion of motilin, so that the composition can further play a role in promoting peristalsis and further play a role in relieving constipation; the important application value is realized.

Description

Technical Field

[0001] This invention relates to the field of biomedical technology, specifically to a composition and its application in the preparation of products that relieve constipation by promoting intestinal peristalsis. Background Technology

[0002] Constipation is mainly characterized by reduced bowel movement frequency, hard stools, and difficulty in defecation. In modern life, constipation has become a common digestive system problem, seriously affecting people's quality of life. Studies have shown that slow intestinal motility is a significant contributing factor to constipation. Under normal circumstances, the intestines push food residue towards the anus through regular peristalsis. If intestinal motility slows down, food residue stays in the intestines for a longer period, leading to excessive water absorption and thus constipation. Current treatments for constipation include the use of chemical drugs, dietary changes, and increased physical activity. However, chemical drugs have a series of side effects, such as dependence and electrolyte imbalance, and long-term use poses potential risks to human health. In contrast, natural active ingredients, due to their natural, safe, and fewer side effects, are receiving increasing attention in the field of constipation relief and treatment.

[0003] Motilin is a polypeptide gastrointestinal hormone secreted by endocrine cells in the digestive tract; it is one of the key hormones regulating intestinal motility, and its secretion rhythm and level directly affect intestinal motility and emptying efficiency. In developing functional products or drugs that promote intestinal motility, increasing motilin secretion or enhancing its activity is an important pathway.

[0004] Plant extracts have gained widespread attention in the fields of functional foods and adjuvant therapies due to their multi-target effects, minimal side effects, and ease of acceptance. Honeysuckle, the dried flower buds or newly opened flowers of the honeysuckle plant (Lonicera japonica, Caprifoliaceae family), is sweet and cold in nature, and enters the lung, heart, and stomach meridians. Traditional Chinese medicine uses it to clear heat and detoxify, and disperse wind-heat. Modern pharmacological studies have shown that honeysuckle is rich in phenolic acids and flavonoids such as chlorogenic acid, luteolin, and isochlorogenic acid, exhibiting significant anti-inflammatory, antioxidant, gut microbiota-regulating, and intestinal barrier-improving effects. Hawthorn, the mature fruit of the hawthorn plant (Rosaceae family), is sour, sweet, and slightly warm in nature, and enters the spleen, stomach, and liver meridians. Its traditional effects include promoting digestion, strengthening the stomach, and dispersing blood stasis. Its main active ingredients include flavonoids (such as quercetin and hyperoside), organic acids (such as citric acid, malic acid, and hawthorn acid), and pectin.

[0005] Although honeysuckle and hawthorn each have certain applications in digestive health, there are no reports in the current technology of scientifically combining their extracts to promote motilin secretion and thus promote intestinal peristalsis and relieve constipation. Summary of the Invention

[0006] In order to overcome at least one of the technical problems existing in the prior art, the present invention provides a composition and its use in the preparation of a product that relieves constipation by promoting intestinal peristalsis.

[0007] The technical solution of the present invention is as follows: The present invention first provides a composition comprising honeysuckle extract and hawthorn extract; wherein the mass ratio of honeysuckle extract and hawthorn extract is 1:(1-80).

[0008] This invention provides a novel composition of honeysuckle extract and hawthorn extract. Studies have shown that the composition obtained by combining honeysuckle extract and hawthorn extract prepared by the method described in this invention has a significantly higher effect on promoting motilin secretion than either honeysuckle extract or hawthorn extract alone, thus achieving a synergistic effect.

[0009] Preferably, the mass ratio of honeysuckle extract to hawthorn extract is 1:(5-10).

[0010] The most preferred embodiment is that the mass ratio of honeysuckle extract to hawthorn extract is 1:9.

[0011] Preferably, the honeysuckle extract is prepared by the following method: taking fresh honeysuckle, extracting it with water, filtering it after extraction, and then concentrating and drying the filtrate to obtain the honeysuckle extract.

[0012] Preferably, the ratio of fresh honeysuckle to water is 1kg:5~10L.

[0013] The preferred ratio of fresh honeysuckle to water is 1 kg: 9 L.

[0014] Preferably, the extraction is performed by heating and reflux extraction; the extraction temperature is 80~100℃, and the extraction time is 0.5~3h.

[0015] Most preferably, the extraction is performed by heating and reflux extraction; the extraction temperature is 90°C and the extraction time is 1.5 hours.

[0016] Preferably, the hawthorn extract is prepared by the following method: (1) Take fresh hawthorn, extract it with water, and filter it after extraction to obtain the filtrate; (2) Concentrate the filtrate to obtain a concentrated solution; add ethanol to the concentrated solution to obtain a mixed solution; (3) Let the mixture stand at 1~5℃ for 8~16h. After standing, filter to obtain the precipitate. Take the precipitate to obtain the hawthorn extract.

[0017] Preferably, the ratio of fresh hawthorn to water in step (1) is 1kg:5~10L.

[0018] Most preferably, the ratio of fresh hawthorn to water in step (1) is 1kg:9L.

[0019] Preferably, the extraction in step (1) is a heating reflux extraction, with an extraction temperature of 80~100℃ and an extraction time of 0.5~3h.

[0020] Most preferably, the extraction in step (1) is a heating reflux extraction, with an extraction temperature of 100°C and an extraction time of 1 hour.

[0021] Preferably, in step (2), the filtrate is concentrated to 1 / 4 to 1 / 8 of its volume.

[0022] Most preferably, in step (2), the filtrate is concentrated to 1 / 5 of its volume.

[0023] Preferably, in step (2), ethanol is added to the concentrate to obtain a mixture, such that the volume concentration of ethanol in the mixture is 70-85%.

[0024] Most preferably, in step (2), ethanol is added to the concentrate to obtain a mixture, such that the volume concentration of ethanol in the mixture is 80%.

[0025] Preferably, in step (3), the mixture is left to stand at 4°C for 8 hours.

[0026] Preferably, the hawthorn extract is further prepared by the following method: the precipitate is loaded onto an ion exchange chromatography column, and impurities are removed by elution with a 0.15-0.20M NaCl aqueous solution; then, it is eluted with a 0.3-0.35M NaCl aqueous solution, and the eluent obtained by elution with the 0.3-0.35M NaCl aqueous solution is collected, concentrated and dried to obtain the hawthorn extract.

[0027] Most preferably, the hawthorn extract is further prepared by the following method: the precipitate is loaded onto an ion exchange chromatography column, and impurities are removed by elution with a 0.18M NaCl aqueous solution; then, it is eluted with a 0.32M NaCl aqueous solution, and the eluent obtained by elution with the 0.32M NaCl aqueous solution is collected, concentrated and dried to obtain the hawthorn extract.

[0028] Preferably, the ion exchange column is a DEAE-Sephadex A-25 ion exchange column.

[0029] In further research, the inventors discovered that adding hawthorn extract obtained by elution using the aforementioned ion-exchange chromatography column to the composition significantly enhanced its motilin-promoting effect compared to adding hawthorn extract obtained by elution using a column other than the one described above.

[0030] The inventors also discovered in their research that the elution conditions of the ion exchange chromatography column described in this invention are crucial; the hawthorn extract obtained by elution under the ion exchange chromatography column elution conditions described in this invention must be added to the composition for its motilin-promoting effect to be further significantly enhanced; however, the addition of hawthorn extract obtained by elution under other ion exchange chromatography column elution conditions does not result in a further significant enhancement of its motilin-promoting effect.

[0031] The present invention also provides the use of the above composition in the preparation of a product having the effect of promoting motilin secretion.

[0032] The present invention also provides the use of the above composition in the preparation of a product having the effect of promoting intestinal peristalsis.

[0033] The present invention also provides the use of the above composition in the preparation of a product that relieves constipation by promoting intestinal peristalsis.

[0034] Beneficial Effects: This invention provides a novel composition of honeysuckle extract and hawthorn extract. Studies have shown that the composition obtained by combining the honeysuckle extract and hawthorn extract prepared by the method described in this invention has a significantly higher motilin-promoting effect than either honeysuckle extract or hawthorn extract alone, exhibiting a synergistic effect. Because the composition of this invention promotes motilin secretion, it can further promote intestinal peristalsis, thereby relieving constipation; thus, it has significant application value. Detailed Implementation

[0035] The present invention will be further described in detail below with reference to specific embodiments, but the embodiments do not limit the present invention in any way.

[0036] Example 1 Preparation of the composition The composition is obtained by mixing honeysuckle extract and hawthorn extract at a mass ratio of 1:9.

[0037] The honeysuckle extract is prepared by the following method: take fresh honeysuckle, heat water to 90℃ for extraction for 1.5h, filter after extraction to obtain filtrate; concentrate and dry the filtrate to obtain the honeysuckle extract; wherein, the ratio of fresh honeysuckle to water is 1kg:9L.

[0038] The hawthorn extract was prepared by the following method: (1) Take fresh hawthorn, heat water to 90℃ for 1.5h extraction, filter after extraction to obtain filtrate; wherein, the ratio of fresh hawthorn to water is 1kg:9L; (2) Concentrate the filtrate to 1 / 5 of its volume to obtain a concentrated solution; add ethanol to the concentrated solution to obtain a mixed solution; wherein the volume concentration of ethanol in the mixed solution is 80%; (3) The mixture was allowed to stand at 4°C for 8 hours. After standing, the precipitate was obtained by filtration. The precipitate was then used to obtain the hawthorn extract.

[0039] Example 2 Preparation of the composition The composition is obtained by mixing honeysuckle extract and hawthorn extract at a mass ratio of 1:9.

[0040] The honeysuckle extract is prepared by the following method: take fresh honeysuckle, heat water to 90℃ for extraction for 1.5h, filter after extraction to obtain filtrate; concentrate and dry the filtrate to obtain the honeysuckle extract; wherein, the ratio of fresh honeysuckle to water is 1kg:9L.

[0041] The hawthorn extract was prepared by the following method: (1) Take fresh hawthorn, heat water to 90℃ for 1.5h extraction, filter after extraction to obtain filtrate; wherein, the ratio of fresh hawthorn to water is 1kg:9L; (2) Concentrate the filtrate to 1 / 5 of its volume to obtain a concentrated solution; add ethanol to the concentrated solution to obtain a mixed solution; wherein the volume concentration of ethanol in the mixed solution is 80%; (3) The mixture was allowed to stand at 4°C for 8 hours. After standing, the precipitate was obtained by filtration. (4) Take the precipitate, load it onto a DEAE-Sephadex A-25 ion exchange column, first elute with a 0.18M NaCl aqueous solution to remove impurities; then elute with a 0.32M NaCl aqueous solution, collect the eluent eluted with the 0.32M NaCl aqueous solution, concentrate and dry it to obtain the hawthorn extract.

[0042] Comparative Example 1: Preparation of the Composition The composition is obtained by mixing honeysuckle extract and hawthorn extract at a mass ratio of 1:9.

[0043] The honeysuckle extract is prepared by the following method: take fresh honeysuckle, heat water to 90℃ for extraction for 1.5h, filter after extraction to obtain filtrate; concentrate and dry the filtrate to obtain the honeysuckle extract; wherein, the ratio of fresh honeysuckle to water is 1kg:9L.

[0044] The hawthorn extract was prepared by the following method: (1) Take fresh hawthorn, heat water to 90℃ for 1.5h extraction, filter after extraction to obtain filtrate; wherein, the ratio of fresh hawthorn to water is 1kg:9L; (2) Concentrate the filtrate to 1 / 5 of its volume to obtain a concentrated solution; add ethanol to the concentrated solution to obtain a mixed solution; wherein the volume concentration of ethanol in the mixed solution is 80%; (3) The mixture was allowed to stand at 4°C for 8 hours. After standing, the precipitate was obtained by filtration. (4) Take the precipitate, load it onto a DEAE-Sephadex A-25 ion exchange column, first elute with a 0.08M NaCl aqueous solution to remove impurities; then elute with a 0.2M NaCl aqueous solution, collect the eluent eluted with the 0.2M NaCl aqueous solution, concentrate and dry it to obtain the hawthorn extract.

[0045] Comparative Example 2: Preparation of the Composition The composition is obtained by mixing honeysuckle extract and hawthorn extract at a mass ratio of 1:9.

[0046] The honeysuckle extract is prepared by the following method: take fresh honeysuckle, heat water to 90℃ for extraction for 1.5h, filter after extraction to obtain filtrate; concentrate and dry the filtrate to obtain the honeysuckle extract; wherein, the ratio of fresh honeysuckle to water is 1kg:9L.

[0047] The hawthorn extract was prepared by the following method: (1) Take fresh hawthorn, heat water to 90℃ for 1.5h extraction, filter after extraction to obtain filtrate; wherein, the ratio of fresh hawthorn to water is 1kg:9L; (2) Concentrate the filtrate to 1 / 5 of its volume to obtain a concentrated solution; add ethanol to the concentrated solution to obtain a mixed solution; wherein the volume concentration of ethanol in the mixed solution is 80%; (3) The mixture was allowed to stand at 4°C for 8 hours. After standing, the precipitate was obtained by filtration. (4) Take the precipitate, load it onto a DEAE-Sephadex A-25 ion exchange column, first elute with a 0.35M NaCl aqueous solution to remove impurities; then elute with a 0.5M NaCl aqueous solution, collect the eluent obtained from the 0.5M NaCl aqueous solution, concentrate and dry it to obtain the hawthorn extract. Example 4: Evaluation of the composition's activity The test samples in this experimental example are honeysuckle extract prepared according to the method of Example 1, hawthorn extract prepared according to the method of Example 1, and compositions prepared in Examples 1-2 and Comparative Examples 1-2.

[0048] (1) Dissolve the sample to be tested in distilled water to prepare a solution with a concentration of 1 g / mL.

[0049] (2) Healthy female Kunming mice (19-22 g) aged 5 weeks were acclimatized for 1 week and then randomly divided into groups of 8 mice each. Except for the blank control group, the other groups of mice were administered loperamide by gavage at a dose of 10 mg / kg once a day for 7 consecutive days to inhibit intestinal peristalsis. From the second day after the start of modeling, the mice in the test drug intervention group were administered the active ingredient by gavage at a dose of 0.2 mL / mouse / day once a day for 7 consecutive days, while the blank control group and the model group were administered an equal volume of physiological saline by gavage. 24 hours after the end of administration on the 7th day, the motilin content in the mouse serum was detected by enzyme-linked immunosorbent assay (ELISA); the motilin content in the blank group was set as 100%, and the motilin content in the other groups was a relative value; the results are shown in Table 1.

[0050] Experimental groups: Blank group (no treatment); Model group (loperamide by gavage); Experimental group 1 (loperamide by gavage and honeysuckle extract prepared by the method of Example 1), Experimental group 2 (loperamide by gavage and hawthorn extract prepared by the method of Example 1); Experimental group 3 (loperamide by gavage and the composition prepared by Example 1); Experimental group 4 (loperamide by gavage and the composition prepared by Example 2); Experimental group 5 (loperamide by gavage and the composition prepared by Comparative Example 1); Experimental group 6 (loperamide by gavage and the composition prepared by Comparative Example 2).

[0051] Table 1. Results of the activity test of the composition Sample to be tested Relative content of motilin Blank group - 100±9.63 Model group - 70.9±6.53 Experimental group 1 Honeysuckle extract prepared by the method in Example 1 82.8±6.93 Experimental group 2 Hawthorn extract prepared by the method in Example 1 78.2±6.90 Experimental group 3 The composition prepared in Example 1 95.4±7.27 Experimental group 4 The composition prepared in Example 2 128.6±8.85 Experimental group 5 The composition prepared in Comparative Example 1 99.8±7.03 Experimental group 6 The composition prepared in Comparative Example 2 10.1±7.85 As can be seen from the experimental results in Table 1, the relative content of motilin in experimental group 3 was significantly higher than that in experimental groups 1 and 2. This indicates that the combination of honeysuckle extract and hawthorn extract prepared by the method described in this invention has a significantly higher motilin-promoting effect than either honeysuckle extract or hawthorn extract alone. The combination of honeysuckle extract and hawthorn extract prepared by the method described in this invention can synergistically promote motilin secretion.

[0052] As can be seen from the experimental results in Table 1, the relative content of motilin in experimental group 4 was significantly higher than that in experimental group 3. This indicates that adding hawthorn extract obtained by elution using the ion exchange chromatography column of the present invention to the composition further significantly enhances its motilin secretion-promoting effect compared to adding hawthorn extract obtained by elution without elution using the ion exchange chromatography column of the present invention.

[0053] The experimental results in Table 1 also show that although the relative motilin content in experimental groups 5 and 6 was higher than that in experimental group 3, the increase was not significant, and the increase was much smaller than that in experimental group 4. This indicates that the elution conditions of the ion exchange chromatography column described in this invention are crucial. The addition of hawthorn extract eluted using the ion exchange chromatography column conditions described in this invention is necessary to significantly enhance the motilin secretion-promoting effect compared to adding hawthorn extract eluted without the aforementioned ion exchange chromatography column conditions. However, adding hawthorn extract eluted using other ion exchange chromatography column conditions does not significantly enhance the motilin secretion-promoting effect.

Claims

1. A composition, characterized in that, It contains honeysuckle extract and hawthorn extract; wherein the mass ratio of honeysuckle extract to hawthorn extract is 1:(1-80).

2. The composition according to claim 1, characterized in that, The mass ratio of honeysuckle extract to hawthorn extract is 1:(5-10); The most preferred embodiment is that the mass ratio of honeysuckle extract to hawthorn extract is 1:

9.

3. The composition according to claim 1, characterized in that, The honeysuckle extract is prepared by the following method: fresh honeysuckle is taken and extracted with water. After extraction, the filtrate is filtered to obtain the filtrate. The filtrate is concentrated and dried to obtain the honeysuckle extract.

4. The composition according to claim 1, characterized in that, The ratio of fresh honeysuckle to water is 1kg:5~10L; The preferred ratio of fresh honeysuckle to water is 1 kg: 9 L.

5. The composition according to claim 3, characterized in that, The extraction method is reflux extraction; the extraction temperature is 80~100℃, and the extraction time is 0.5~3h.

6. The composition according to claim 1, characterized in that, The hawthorn extract was prepared by the following method: (1) Take fresh hawthorn, extract it with water, and filter it after extraction to obtain the filtrate; (2) Concentrate the filtrate to obtain a concentrated solution; add ethanol to the concentrated solution to obtain a mixed solution; (3) Let the mixture stand at 1~5℃ for 8~16h. After standing, filter to obtain the precipitate. Take the precipitate to obtain the hawthorn extract.

7. The composition according to claim 6, characterized in that, In step (1), the ratio of fresh hawthorn to water is 1kg:5~10L.

8. Use of the composition according to any one of claims 1 to 7 in the preparation of a product having the effect of promoting motilin secretion.

9. Use of the composition according to any one of claims 1 to 7 in the preparation of a product having the effect of promoting intestinal peristalsis.

10. Use of the composition according to any one of claims 1 to 7 in the preparation of a product that relieves constipation by promoting intestinal peristalsis.