Artemisia annua extract, its preparation method and application and artemisia annua extract veterinary medicine

Abstract

The application discloses a sweet wormwood extract, a preparation method and application thereof and a sweet wormwood extract veterinary drug, and the method comprises the following steps: S1, heating and extracting sweet wormwood byproducts with an extraction solvent, filtering, discarding the filtrate, and obtaining an intermediate product filter cake; S2, concentrating the intermediate product filter cake to a constant weight; S3, heating and extracting the constant weight obtained in the step S2 with deionized water, filtering, and taking the filtrate; S4, concentrating the filtrate obtained in the step S3 to a constant weight, and obtaining the sweet wormwood extract. The application provides a sweet wormwood extract and a preparation method thereof, and provides a veterinary drug based on the sweet wormwood extract; the veterinary drug obtained by the application can clear heat, resolve summer-heat, and relieve deficiency heat, and can be used for treating various livestock and poultry diseases; the preparation method is simple, easy to operate, raw materials are easy to obtain, production cost is low, and the application has a good market application prospect.

Description

Technical Field

[0001] This invention relates to the field of artemisia, and particularly to an artemisia extract, its preparation method and application, and artemisia extract as a veterinary drug. Background Technology

[0002] Veterinary drugs are important for the prevention and control of animal diseases. They can effectively prevent the spread of diseases and establish a healthier animal husbandry system. With the continuous development of my country's animal husbandry industry, animal diseases are becoming more and more common. Strengthening disease control has become an indispensable part of modern animal husbandry management.

[0003] Artemisia annua is the aerial part of Artemisia annua, a plant in the Asteraceae family. It has heat-clearing and detoxifying properties and is used for summer heat fever, yin deficiency fever, malaria with chills and fever, and damp-heat jaundice. In large-scale modern livestock and poultry farming, Artemisia annua powder has broad application prospects in the targeted control of tissue protozoa (also known as medical protozoa), such as poultry white comb disease (including white comb disease), and also in enhancing the body's immune system function.

[0004] Therefore, it is very important to develop more dosage form preparation methods for veterinary drugs. Summary of the Invention

[0005] The technical problem to be solved by the present invention is to address the shortcomings of the prior art by providing an artemisia annua extract, its preparation method and application, and an artemisia annua extract veterinary drug.

[0006] To solve the above-mentioned technical problems, the technical solution adopted by the present invention is as follows: In its first aspect, the present invention provides a method for preparing artemisia annua extract, comprising:

[0007] S1. Heat and extract the Artemisia annua byproducts with an extraction solvent, filter, discard the filtrate, and obtain the intermediate product filter cake.

[0008] S2. Concentrate the intermediate product filter cake to constant weight;

[0009] S3. Heat and extract the constant weight substance obtained in step S2 with deionized water, filter, and collect the filtrate.

[0010] S4. Concentrate the filtrate obtained in step S3 to constant weight to obtain artemisia annua extract.

[0011] Preferably, the extraction temperature in step S1 is 70–90°C, the extraction time is 1–3 hours, and the extraction is performed 1–3 times.

[0012] Preferably, the extraction solvent in step S1 is at least one of methanol, ethanol, petroleum ether, and ethyl acetate.

[0013] Preferably, the mass ratio of the extraction solvent to the artemisia annua byproduct in step S1 is 5–15:1.

[0014] Preferably, the concentration temperature in steps S2 and S4 is 60–90°C.

[0015] Preferably, the extraction temperature in step S3 is 70–90°C, the extraction time is 1–3 hours, and the extraction is performed 1–3 times.

[0016] Preferably, the mass ratio of deionized water to constant weight in step S3 is 5 to 20:1.

[0017] In a second aspect, the present invention provides an artemisia annua extract, which is prepared by the method described above.

[0018] A third aspect of the present invention provides the application of the artemisia annua extract as described above in the preparation of veterinary drugs.

[0019] In a fourth aspect, the present invention provides a veterinary drug made from artemisia annua extract, which is prepared by the following method:

[0020] 1) Add the artemisia annua extract as described above to the binder dextrin, the mass ratio of the binder dextrin is 1-5%, mix well, and make into granules;

[0021] 2) Dry the granules to obtain the Artemisia annua extract veterinary drug.

[0022] The beneficial effects of this invention are:

[0023] This invention provides an artemisia annua extract and its preparation method, and provides a veterinary drug based on the artemisia annua extract. The veterinary drug obtained by this invention can clear heat, relieve summer heat and reduce deficiency fever, and can be used to treat a variety of livestock and poultry diseases. The preparation process of this invention is simple, easy to operate, the raw materials are readily available, the production cost is low, and it has a good market application prospect. Detailed Implementation

[0024] The present invention will be further described in detail below with reference to embodiments, so that those skilled in the art can implement it based on the description.

[0025] It should be understood that terms such as “having,” “comprising,” and “including” as used herein do not exclude the presence or addition of one or more other elements or combinations thereof.

[0026] Unless otherwise specified, the experimental methods used in the following examples are conventional methods. Unless otherwise specified, the materials and reagents used in the following examples are commercially available. For examples where specific conditions are not specified, conventional conditions or conditions recommended by the manufacturer are followed. For reagents or instruments whose manufacturers are not specified, they are all commercially available products.

[0027] This invention provides an artemisia annua extract and a method for preparing the same, the method comprising:

[0028] S1. Heat and extract the Artemisia annua byproducts with an extraction solvent, filter, discard the filtrate, and obtain the intermediate product filter cake.

[0029] S2. Concentrate the intermediate product filter cake to constant weight;

[0030] S3. Heat and extract the constant weight substance obtained in step S2 with deionized water, filter, and collect the filtrate.

[0031] S4. Concentrate the filtrate obtained in step S3 to constant weight to obtain artemisia annua extract.

[0032] In a preferred embodiment, the extraction temperature in step S1 is 70-90°C, the extraction time is 1-3 hours, and the extraction is performed 1-3 times.

[0033] In a preferred embodiment, the extraction solvent in step S1 is at least one of methanol, ethanol, petroleum ether, and ethyl acetate.

[0034] In a preferred embodiment, the mass ratio of the extraction solvent to the artemisia byproduct in step S1 is 5 to 15:1.

[0035] In a preferred embodiment, the concentration temperature in steps S2 and S4 is 60–90°C.

[0036] In a preferred embodiment, the extraction temperature in step S3 is 70-90°C, the extraction time is 1-3 hours, and the extraction is performed 1-3 times.

[0037] In a preferred embodiment, the mass ratio of deionized water to constant weight in step S3 is 5 to 20:1.

[0038] The present invention also provides an artemisia annua extract, which is prepared by the method described above.

[0039] The present invention also provides the application of the artemisia annua extract as described above in the preparation of veterinary drugs.

[0040] This invention also provides a veterinary drug made from artemisia annua extract, characterized in that it is prepared by the following method:

[0041] 1) Add the artemisia annua extract as described above to the binder dextrin, the mass ratio of the binder dextrin is 1-5%, mix well, and make into granules;

[0042] 2) Dry the granules to obtain the Artemisia annua extract veterinary drug.

[0043] The artemisia byproducts in this invention are specifically: artemisia byproducts are enriched products after artemisinin is separated from artemisinin by column chromatography from artemisia extract.

[0044] The extracted Artemisia annua extract contains various chemical components such as sesquiterpenes, diterpenes, flavonoids, phenylpropionic acid, coumarins, and volatile oils. These components give it the effects of clearing heat and relieving summer heat, and reducing deficiency heat. After being prepared into veterinary drugs as active ingredients, it can be used to treat various livestock and poultry diseases such as fever due to summer heat, fever due to yin deficiency, malaria with chills and fever, and damp-heat jaundice. For example, the literature "Niu Biao, Qin Wenwen, Guo Wenzhu, et al. Study on the efficacy of Artemisia annua powder against coccidiosis in chickens [J]. China Animal Husbandry and Veterinary Medicine, 2018, 45(6):9.DOI:CNKI:SUN:GWXK.0.2018-06-033." and "Song Ping, Ma Qingtao, Su Yangyang, et al. Experiment on the prevention and treatment of Eimeria tenella infection in chickens by Artemisinin-based drugs [J]. China Poultry, 2014, 36(5):2.DOI :10.3969 / j.issn.1004-6364.2014.05.011.》《Huang Xin, Guo Wenzhu, Gao Xudong, et al. Research progress on the source of artemisinin and its mechanism of action against coccidiosis in chickens[J]. Chinese Journal of Preventive Veterinary Medicine, 2016, 38(006):503-506.DOI:10.3969 / j.issn.1008-0503.2016.06.19.》

[0045] The above is the general concept of the present invention. Based on this, detailed embodiments and comparative examples are provided below to further illustrate the present invention.

[0046] Example 1

[0047] An artemisia annua extract and its preparation method, the method comprising:

[0048] S1. Weigh 1 kg of Artemisia annua byproduct (the enriched product after separating artemisinin from Artemisia annua extract by column chromatography, the same applies below) and 10 times the mass of ethanol. Heat and extract twice at 80℃, 1 hour each time, to obtain 0.62 kg of light yellow semi-solid, which is the intermediate product filter cake.

[0049] S2. Concentrate 0.62 kg of filter cake to constant weight at 70°C to obtain 0.58 kg of constant weight.

[0050] S3. Heat 0.58 kg of semi-solid with 10 times its weight of water at 80°C twice for 1 hour each time, and combine the two filtrates.

[0051] S4. Concentrate the filtrate to constant weight to obtain 0.28 kg of artemisia annua extract.

[0052] A veterinary drug made from artemisia annua extract is prepared by the following method:

[0053] 1) Add 0.28 kg of Artemisia annua extract to 8.4 g of binder dextrin, mix well, and make into granules;

[0054] 2) Dry the granules to obtain the Artemisia annua extract veterinary drug.

[0055] Example 2

[0056] An artemisia annua extract and its preparation method, the method comprising:

[0057] S1. Weigh 50 kg of Artemisia annua byproduct and 10 times its weight of ethanol, and heat and extract twice at 80°C for 1 hour each time to obtain 30.5 kg of light yellow semi-solid, which is the intermediate product filter cake.

[0058] S2. Concentrate 30.5 kg of filter cake to constant weight at 70°C to obtain 28.6 kg of constant weight.

[0059] S3. Heat 28.6 kg of semi-solid with 10 times its weight of water at 80°C twice for 1 hour each time, and combine the two filtrates.

[0060] S4. Concentrate the filtrate to constant weight to obtain 13.7 kg of artemisia annua extract.

[0061] A veterinary drug made from artemisia annua extract is prepared by the following method:

[0062] 1) Add 13.7 kg of Artemisia annua extract to 0.42 kg of binder dextrin, mix well, and prepare granules;

[0063] 2) Dry the granules to obtain the Artemisia annua extract veterinary drug.

[0064] Example 3

[0065] An artemisia annua extract and its preparation method, the method comprising:

[0066] S1. Weigh 100 kg of Artemisia annua byproduct and 10 times the mass of ethanol, and heat and extract twice at 80℃ for 1 hour each time to obtain 59.1 kg of light yellow semi-solid, which is the intermediate product filter cake.

[0067] S2. The 59.1 kg filter cake was concentrated to constant weight at 70°C to obtain 55.8 kg of constant weight.

[0068] S3. Heat 55.8 kg of semi-solid with 10 times its weight of water at 80°C twice for 1 hour each time, and combine the two filtrates.

[0069] S4. Concentrate the filtrate to constant weight to obtain 25.6 kg of artemisia annua extract.

[0070] A veterinary drug made from artemisia annua extract is prepared by the following method:

[0071] 1) Add 25.6 kg of Artemisia annua extract to 0.84 kg of binder dextrin, mix well, and make granules;

[0072] 2) Dry the granules to obtain the Artemisia annua extract veterinary drug.

[0073] Veterinary drug treatment efficacy test

[0074] Using Eimeria coccidiosis in chicks as a model, relative survival rate, cecal lesion value, and cecal oocysts were used as observation indicators. Norfloxacin was compared with Examples 1-3, while the control group received no medication.

[0075] 15-day-old chicks were randomly divided into 4 groups of 10 chicks each. One group served as the control (infected with parasites, but not treated). The rest were used to simulate the chick Eimeria coccidiosis model. Medication was administered 24 hours after infection, twice daily for 8 consecutive days. Chicks were observed daily after treatment. Dead chicks were necropsy to check for cecal lesions and cecal oocysts. Nine days later, the chicks were weighed and their cecal lesions and cecal oocysts were checked again.

[0076] The effects of norfloxacin (Examples 1-3) on Eimeria coccidiosis were compared, and the results are shown in Table 1 below:

[0077] Table 1

[0078] Group Surviving (only) Cecal lesion value Cecal ovary value Anticoccidial Index Anticoccidial effect control group 7 6.72 10 86 - Norfloxacin 10 1.84 0 199 efficient Example 1 10 1.78 0 188 efficient Example 2 10 1.81 0 189 efficient Example 3 10 1.86 0 186 efficient

[0079] Test results showed that the veterinary drugs derived from Artemisia annua extract in Examples 1-3, along with norfloxacin, exhibited higher insect resistance indices than the control group in terms of survival, cecal lesion value, and cecal oocyst value, indicating that they all had good insect resistance effects.

[0080] Although the embodiments of the present invention have been disclosed above, they are not limited to the applications listed in the specification and embodiments. They can be applied to various fields suitable for the present invention. For those skilled in the art, other modifications can be easily made. Therefore, without departing from the general concept defined by the claims and their equivalents, the present invention is not limited to the specific details.

Claims

1. A veterinary drug containing artemisia annua extract, characterized in that, It is prepared by the following method: 1) Add artemisia annua extract to binder dextrin, the mass ratio of binder dextrin is 1-5%, mix well, and make into granules; 2) Dry the granules to obtain the Artemisia annua extract veterinary drug; The Artemisia annua extract is used as a veterinary drug to treat Eimeria coccidiosis. The artemisia extract was prepared by the following method: S1. Heat and extract the Artemisia annua byproducts with an extraction solvent, filter, discard the filtrate, and obtain the intermediate product filter cake. S2. Concentrate the intermediate product filter cake to constant weight; S3. Heat and extract the constant weight substance obtained in step S2 with deionized water, filter, and collect the filtrate. S4. Concentrate the filtrate obtained in step S3 to constant weight to obtain artemisia annua extract; The extraction solvent in step S1 is at least one of methanol and ethanol; The Artemisia annua byproduct is the enriched product after separating artemisinin from Artemisia annua extract by column chromatography. The extraction temperature in step S1 is 70-90℃, the concentration temperature in steps S2 and S4 is 60-90℃, and the extraction temperature in step S3 is 70-90℃.

2. The method for preparing artemisia annua extract according to claim 1, characterized in that, The extraction time in step S1 is 1 to 3 hours, and the number of extractions is 1 to 3.

3. The veterinary drug based on artemisia annua extract according to claim 1, characterized in that, The mass ratio of the extraction solvent to the Artemisia annua byproduct in step S1 is 5–15:

1.

4. The veterinary drug based on artemisia annua extract according to claim 1, characterized in that, The extraction time in step S3 is 1 to 3 hours, and the number of extractions is 1 to 3.

5. The veterinary drug based on artemisia annua extract according to claim 1, characterized in that, In step S3, the mass ratio of deionized water to constant weight is 5 to 20:1.